WO2017214905A1

WO2017214905A1 - Reagent kit having cooling function, loading reminder function, and wireless management function

Info

Publication number: WO2017214905A1
Application number: PCT/CN2016/085920
Authority: WO
Inventors: 彭鹏
Original assignee: 彭鹏
Priority date: 2016-06-15
Filing date: 2016-06-15
Publication date: 2017-12-21

Abstract

A reagent kit having a cooling function, loading reminder function, and wireless management function. By providing a control unit, a temperature sensor, a cooling device (40), and a storage battery to form a cooling system, and providing a sensor (60), a pipette tip collector (32), a reagent turntable (36), a power mechanism, and a near field communication (NFC) tag (80), the present invention provides continuous and uniform refrigeration of the reagent kit when the reagent kit is not in a low-temperature environment, thus providing a favorable low-temperature environment for reagent loading, and preventing a reagent from degrading and becoming unsuitable for use due to loss of a low-temperature environment. At the same time, by driving rotation of reagent recesses (38) with a reagent turntable, the present invention achieves an effect of a loading reminder for lab personnel, thereby preventing an experiment failure due to a loading error. In addition, by employing contactless end-to-end data transmission between an intelligent management terminal (90) and the reagent kit within a five-centimeter range, the present invention solves numerous problems of manufacturing management methods of conventional reagent kits, thus improving efficiency and precision of management, and reducing a management cost of the manufacturer.

Description

Instruction manual Name: A kit with cold preservation, sample reminder and wireless management function

[0001] The present invention relates to the field of biotechnology, and more particularly to a kit having a cold retention, a sample reminder, and a wireless management function.

Background technique

[0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.

technical problem

[0003] Due to the biological characteristics, many reagents in the use of hydrazine to ensure the effectiveness of the reagents and the accuracy of the experiment, need to be completed in a low temperature (2-8 ° C) environment, and currently, such operations are usually used " The ice box is "completed, the ice cubes are added to the appropriate size of the foam box, and the reagent tube is inserted into the ice to ensure that the reagents are at the desired low temperature. However, because many biological experiments require aseptic processing, and ice cubes are easily stained with bacteria in the air, which is a potential source of bacterial contamination in experiments, low-temperature loading operations using "ice boxes" are used in these experiments.吋 The requirements for the operation of the experimenter are higher, the burden on the experimenter is increased, and the experimental success rate cannot be guaranteed.

[0004] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the step of adding samples is becoming more and more complicated. Take the commonly used real-time PCR kit as an example. The general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked.吋 Handling other urgent matters and causing ongoing experiments to be interrupted, errors are inevitable.

[0005] And with the development of biology and the interweaving of biology with other closely related disciplines As the number of kits increases, the number of kits continues to increase. For the manufacturers of kits, especially for large manufacturers that produce a wide variety of widely used kits, the management of the kits produced has always been a very challenging task. At present, most manufacturers use the management method to record the relevant information of the kit in the paper document, and find the relevant documents to check if necessary. This manual recording and comparison management mode results in inefficient and high error rates for kit management, and the additional expenditure for "correction" generated each year amounts to millions or even tens of millions. Problem solution

Technical solution

In order to solve the above problems in the prior art, the present invention provides a kit having a cold retention, a sample reminder, and a wireless management function.

[0007] The technical solution adopted by the invention is: a kit with cold preservation, sample reminding and wireless management functions, comprising a kit body and a lid, wherein the reagent box body is provided with a plurality of reagent holes and temperature sensors , several cooling devices and NFC tags for short-range wireless communication. The control unit is provided with a control unit and a small battery, and the control unit, the cooling device, the temperature sensor and the battery are electrically connected. The near-field wireless communication NFC tag is composed of a data storage module and a data transmission module, and the output end of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal used by the worker through the NFC chip.

[0008] The inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further arranged between the two. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.

[0009] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.

[0010] Further, the kit body has a two-layer structure, and the cooling device is disposed at equal intervals between the inner layer and the outer layer of the kit body.

[0011] Further, the kit body is provided with four cooling devices at equal intervals on each side.

[0012] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring. [0013] Further, the reagent rotating disc is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.

[0014] Further, the arrow indicator is made of LED, connected to the sensor, and controlled by the sensor.

[0015] Further, the smart terminal used by the staff is set on the mobile phone and displays information about the kit through the mobile phone interface.

Advantageous effects of the invention

Beneficial effect

[0016] The present invention provides a two-layer structure, and a cooling device is disposed between the inner layer and the outer layer of the reagent cartridge body, and a cooling system is formed by the control unit, the temperature sensor cooling device, and the storage battery, and the reagent cartridge can be Loss of low temperature environment, continuous and even cooling of the kit, providing a good low temperature environment for reagent loading, and avoiding the deterioration of reagents due to loss of low temperature environment. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the experiment failure caused by the wrong sample; and the intelligent management terminal and the kit can be within a distance of five centimeters. The realization of non-contact point-to-point data transmission solves many problems of the traditional kit production management method, improves the efficiency and accuracy of management, and reduces the management cost for the production enterprise.

Brief description of the drawing

DRAWINGS

1 is an overall configuration diagram of the present invention.

2 is a schematic view showing the structure of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.

3 is a schematic structural diagram of the short-range wireless communication NFC tag.

BEST MODE FOR CARRYING OUT THE INVENTION

[0020] The present invention will be further described below in conjunction with the accompanying drawings.

[0021] As shown in FIG. 1, a kit having a cold preservation, a sample reminding, and a wireless management function includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 and a temperature sensor ( Not shown in the figure) A plurality of cooling devices 40 and a short-range wireless communication NFC tag 80. The kit body has a control unit (not shown) and a small battery (not shown). The control unit, the cooling device, the temperature sensor and the battery are electrically connected.

[0022] As shown in FIG. 2, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is further disposed therebetween, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. The sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32. The rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.

As shown in FIG. 3, the short-range wireless communication NFC tag 80 mainly includes a data storage module 801, a data transmission module 802, and an NFC chip 803. The output of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal (usually the mobile phone 90) used by the user through the NFC chip.

[0024] The kit with the cooling function and the sample reminding function comprises a cooling unit composed of a control unit, a temperature sensor cooling device and a battery, and an inductor, a pipette tip collecting hole, a reagent rotating disk and The power mechanism can continuously and uniformly cool the kit after the kit loses the low temperature environment, and provides a good low temperature environment for the reagent sample to avoid the deterioration of the reagent due to the loss of the low temperature environment. Similarly, the reagent tray drives the reagent. The rotation of the hole can be used as a reminder for the experimenter to avoid the failure of the experiment caused by the wrong sample. In addition, the intelligent management terminal and the kit can realize the non-contact point-to-point data transmission within a distance of five centimeters. It solves many problems of the traditional kit production management method, improves the efficiency and accuracy of management, and reduces the management cost for the production enterprise.

[0025] The present invention is not limited to the above-described preferred embodiments, and anyone else can derive other It is within the scope of the present invention to provide a product of any kind, regardless of its shape or structure, and any technical solution having the same or similar approximation as the present application.

Industrial applicability

The invention can reduce the low temperature environment in the kit by setting the kit body into a double layer structure, and providing a cooling device between the inner layer and the outer layer of the reagent box body, and the cooling system is composed of the control unit, the temperature sensor cooling device and the battery.持续 Continuously and evenly cool the kit to provide a good low temperature environment for reagent loading, and avoid the reagents from being deteriorated due to loss of low temperature environment. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter, avoiding the experiment failure caused by the wrong sample; and the intelligent management terminal and the kit can be within a distance of five centimeters. The realization of non-contact point-to-point data transmission solves many problems of the traditional kit production management method, improves the efficiency and accuracy of management, and reduces the management cost for the production enterprise.

Claims

claims

[Claim 1] A reagent kit with cold preservation, sample addition reminder and wireless management functions, characterized in that it includes a reagent kit body and a lid, and the reagent kit body is provided with a liner, a temperature sensor, a number of cooling devices and Near field wireless communication NFC tag, the test kit body is equipped with a control unit and a small battery, and there are electrical connections between the control unit, the cooling device, the temperature sensor and the battery.

[Claim 2] A reagent kit with cold storage, sample addition reminder and wireless management functions according to claim 1, characterized in that the lining plate is provided with a pipette tip collection hole and a reagent rotating disk, There is an arrow indicator light between the two. There is a certain distance between the lining plate and the opening of the box body, so that the lining plate and the box body form an open accommodation space; the reagent rotating disk is provided with a plurality of reagent holes, And connected with a power mechanism;

a reagent tube arranged in the reagent hole;

A sensor is provided in the box body, the sensor is arranged corresponding to the bottom of the pipette tip collection hole, and the sensor controls the pipette tip by sensing the bottom of the pipette tip collection hole. The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light. .

[Claim 3] A test kit with cold preservation, sample addition reminder and wireless management functions according to claim 1, the material of the arrow indicator light is LED, characterized in that, the arrow indicator light and the The sensor is connected and can light up red, orange, yellow, green and cyan respectively as needed.

, blue, purple and white eight colors of light.

[Claim 4] A reagent kit with cold preservation, sample addition reminder and wireless management functions according to claim 1, characterized in that the reagent kit body has a double-layer structure, and the cooling devices are arranged at equal intervals between the reagents. Between the inner and outer layers of the box body.

[Claim 5] A kit with cold preservation, sample addition reminder and wireless management functions according to claim 1, characterized in that four cooling devices are provided at equal intervals on each side of the kit body.

[Claim 6] A kit with cold storage, sample addition reminder and wireless management functions according to claim 1, characterized in that the short-range wireless communication NFC tag mainly consists of a data storage module, a data transmission module and a Composed of NFC chip. [Claim 7] A test kit with cold storage, sample addition reminder and wireless management functions according to claim 1, characterized in that the data storage module is electrically connected to the data transmission module, and the data transmission module It is electrically connected to the NFC chip and sends relevant data of the test kit to the intelligent management terminal through wireless signals.