WO2017214826A1

WO2017214826A1 - Reagent kit having cold insulation, alarming, wireless management and sample adding reminding functions

Info

Publication number: WO2017214826A1
Application number: PCT/CN2016/085630
Authority: WO
Inventors: 石庆学
Original assignee: 石庆学
Priority date: 2016-06-13
Filing date: 2016-06-13
Publication date: 2017-12-21

Abstract

A reagent kit having cold insulation, alarming, wireless management and sample adding reminding functions comprises a reagent kit body (10) and a kit cover (20). Sound production devices (40), a control unit, a small storage battery, a near-field wireless communication (NFC) label (80), a sensor (60), a pipette tip collection holes (32), a reagent rotary tray (36) and a power mechanism (70) are disposed on the reagent kit body (10). The reagent kit can provide a needed precooling environment (2-8ºC) to the reagent, can detect the temperature by means of a temperature control probe (382); and once the temperature exceeds a preset range, the sound production devices (40) immediately give alarms to remind the experiment personnel. In addition, the reagent rotary tray (36) drives reagent holes (38) to rotate, so that the experiment personnel can be reminded of adding samples, thereby avoiding experiment failure caused by sample adding errors. By means of non-contact point-to-point data transmission that can be implemented between a smart management terminal (90) and the reagent kit within five centimeters, multiple problems in the conventional reagent kit production management method can be resolved, thereby improving the management efficiency and accuracy, and reducing management costs of a production enterprise.

Description

Title: Kit with cold storage, alarm, wireless management and sample reminder technology

[0001] The present invention relates to the field of biotechnology, and more particularly to a kit having a cold retention, alarm, wireless management, and sample reminding function.

Background technique

[0002] In the field of biotechnology, in addition to technical breakthroughs, it is more important to improve the efficiency of research and experimentation. Among them, the emergence of kits plays an important role. The kit standardizes the steps of common biological experiments, providing the experimenter with the reagents to be prepared and the optimized loading method, which greatly increases the speed of the experimental development and the degree of standardization of the results.

technical problem

[0003] Due to the biological characteristics, many reagents in the use of hydrazine to ensure the effectiveness of the reagents and the accuracy of the experiment, need to be completed in a low temperature (2-8 ° C) environment, and currently, such operations are usually used " The ice box is "completed, the ice cubes are added to the appropriate size of the foam box, and the reagent tube is inserted into the ice to ensure that the reagents are at the desired low temperature. However, because many biological experiments require aseptic processing, and ice cubes are easily stained with bacteria in the air, which is a potential source of bacterial contamination in experiments, low-temperature loading operations using "ice boxes" are used in these experiments.吋 The requirements for the operation of the experimenter are higher, the burden on the experimenter is increased, and the experimental success rate cannot be guaranteed. Another disadvantage of using an "ice box" is that it is not possible to ensure that the reagent tube inserted in it is in the desired temperature environment (2-8 ° C). When the temperature is outside this range, the experimenter is not immediately aware of the situation and may have a certain impact on the experimental results.

[0004] In addition, as the complexity of the experiment is increasing, the number of reagents in the existing kits is also increasing, and the loading step is becoming more and more complicated. Take the commonly used real-time PCR kit as an example. The general kit contains Taq enzyme, buffer, chromogenic dye and sterile ultrapure water. In addition, the reagents required by the experimenter include at least the upstream primer. Downstream primers and template, a total of 7 reagents together to prepare a fluorescent quantitative PCR reaction system. In many cases, the experimenter will have the phenomenon of missing, adding, adding, etc. during the process of adding samples. The reason is that there are many kinds of reagents, and the process of loading cannot be well identified and marked.吋 Handling other urgent matters and causing the ongoing experiment to be interrupted, the error is in the office Inevitably.

[0005] As the development of biology and the interdisciplinary processes of biology and other closely related disciplines increase, the variety of kits continues to increase. For the manufacturers of kits, especially for large manufacturers that produce a wide variety of widely used kits, the management of the kits produced has always been a very challenging task. At present, most manufacturers use the management method to record the relevant information of the kit in the paper document, and find the relevant documents to check if necessary. This manual recording and comparison management mode results in inefficient and high error rates for kit management, and the additional expenditure for "correction" generated each year amounts to millions or even tens of millions.

Problem solution

Technical solution

In order to solve the above problems in the prior art, the present invention provides a kit having a cold storage, an alarm, a wireless management, and a sample reminding function.

[0007] The technical solution adopted by the invention is: a kit with cold preservation, alarm, wireless management and sample reminding function, comprising a kit body and a lid, wherein the kit body is provided with a sounding device and a control Unit, small battery and NFC tag for short-range wireless communication. The sounding device, the control unit and the battery are electrically connected; the outer wall of the reagent hole is provided with a refrigeration device and a temperature control probe, and the refrigeration device, the temperature control probe and the control unit are electrically connected; the short-range wireless communication The NFC tag is composed of a data storage module and a data transmission module. The output end of the data storage module is electrically connected to the data transmission module, and the data transmission module wirelessly transmits the signal to the intelligent management terminal used by the staff through the NFC chip.

[0008] The inner layer of the kit body is further provided with a lining plate, and the lining plate is provided with a pipette tip head collecting hole and a reagent rotating disk, and an arrow indicator light is further arranged between the two. The lining plate is spaced apart from the mouth of the box body such that the lining plate forms a clogging space with the box body; the reagent rotating disk is provided with a plurality of reagent holes, And connected to a power mechanism.

[0009] the reagent box body is further provided with an inductor, the sensor is disposed corresponding to a bottom of the pipette tip head collecting hole, and the sensor receives the hole by sensing the pipette tip head The bottom controls the rotation of the reagent rotating disk and the color of the arrow indicator light.

[0010] Further, the kit body has a two-layer structure, and the control unit, the sounding device and the small battery are disposed between the inner layer and the outer layer of the reagent cartridge body. [0011] Further, the number of the reagent wells is six.

[0012] Further, the sounding device is a buzzer, and the number is two.

[0013] Further, the control unit is a single chip microcomputer.

[0014] Further, the small battery is a lithium ion battery.

[0015] Further, the inductor is connected to the bottom of the pipette tip collecting hole by a spring.

[0016] Further, the reagent rotating disk is provided with 8 reagent holes; the power mechanism connected thereto is a stepping motor, and is connected to the inductor and controlled by the inductor.

Further, the arrow indicator is made of LED, connected to the sensor, and controlled by the sensor.

[0018] Further, the smart terminal used by the staff is set on the mobile phone and displays related information of the kit through the mobile phone interface.

Advantageous effects of the invention

Beneficial effect

[0019] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by the temperature control probe. Once the setting range is exceeded, the sounding device immediately alarms the experimenter to reduce the probability of the experiment failure. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter. Avoid the failure of the experiment caused by the wrong sample; With the intelligent management terminal and the kit, the non-contact point-to-point data transmission can be realized within a distance of five centimeters, which solves many problems of the traditional kit production management method and improves the management. Efficiency and accuracy reduce management costs for manufacturing companies.

Brief description of the drawing

DRAWINGS

1 is an overall configuration diagram of the present invention.

2 is a structural diagram of the reagent well.

3 is a schematic structural view of a reagent rotating disk, an arrow indicator lamp, and a pipette tip head collecting hole as shown in FIG. 1.

4 is a schematic structural diagram of the short-range wireless communication NFC tag. BEST MODE FOR CARRYING OUT THE INVENTION

BEST MODE FOR CARRYING OUT THE INVENTION

[0024] The present invention will be further described below in conjunction with the accompanying drawings.

[0025] As shown in FIG. 1, a kit having a cold preservation, alarm, wireless management, and sample reminding function includes a kit body 10 and a lid 20, and the kit body is provided with a liner 30 and sounding The device 40 and a control unit (not shown), a small battery (not shown), and a short-range wireless communication NFC tag 80. The sounding device, the control unit and the battery are electrically connected.

As shown in FIG. 2, the outer wall of the reagent hole is provided with a refrigerating device 381 and a temperature control probe 382, and the refrigerating device, the temperature control probe and the control unit are electrically connected.

[0027] As shown in FIG. 3, the lining plate 30 is provided with a pipette tip collecting hole 32 and a reagent rotating disk 36, and an arrow indicator light 34 is disposed between the two, and the reagent rotating disk 36 is disposed on the reagent rotating plate 36. Several reagent wells 38. The sensor 60 is disposed in the casing 10, and the inductor 60 is disposed corresponding to the bottom of the pipette tip collecting hole 32, and the inductor 60 controls the stepping motor 70 through the bottom of the inductive pipette tip collecting hole 32. The rotation of the reagent rotating disk 36 and the color of the arrow indicator light 34 are illuminated. With the above arrangement, the inductor 60 is connected to the bottom of the pipette tip head collecting hole 32 by a spring 50. When the loading operation is completed, the discarded tip is driven into the inductive pipette tip head collecting hole 32, and the sensor 60 controls the stepping motor 70 to drive the reagent rotation by inducing the pipette tip to collect the bottom of the hole 32. The color of the disk 36 is rotated and the arrow indicator light 34 is illuminated. The color of the arrow indicator light 34 is in one-to-one correspondence with the reagent holes on the reagent rotating disk 36. The eight colors of red, orange, yellow, green, cyan, blue, purple, and white correspond to 1-8 on the reagent rotating disk 36, respectively. Hole. Each time the sensor senses that the discarded tip is driven into the pipette tip collection hole, the control stepper motor 70 drives the reagent rotating disk 36 to rotate 45° to its next reagent hole, and controls the arrow indicator. 34 lights up the color of the corresponding reagent hole.

[0028] As shown in FIG. 4, the short-range wireless communication NFC tag is composed of a data storage module 801 and a data transmission module 802. The output end of the data storage module is electrically connected to the data transmission module, and the data transmission module passes through the NFC core 803. The signal is transmitted wirelessly to the intelligent management terminal (usually mobile phone 90) used by the staff.

[0029] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by the temperature control probe. Once the setting range is exceeded, the sounding device immediately alerts the experimenter to reduce the probability of the experiment failure. Similarly, the reagent hole can be rotated by the reagent disk, which can be used by the experimenter. To the role of the sample reminder, to avoid the failure of the experiment caused by the wrong sample; With the intelligent management terminal and the kit, the non-contact point-to-point data transmission can be realized within a distance of five centimeters, solving many of the traditional kit production management methods. The problem is to improve the efficiency and accuracy of management and reduce the management cost for the production enterprise.

[0030] The present invention is not limited to the above-described preferred embodiments, and any other form of product can be derived by anyone of the present invention, but without any change in its shape or structure, The same or similar technical solutions are all within the scope of the present invention.

Industrial applicability

[0031] The present invention provides a required pre-cooling environment (2-8 ° C) for the reagent by providing a sounding device, a control unit and a battery in the kit body and a refrigeration device and a temperature control probe on the outer wall of the reagent hole, and The temperature can be detected by the temperature control probe. Once the setting range is exceeded, the sounding device immediately alarms the experimenter to reduce the probability of the experiment failure. At the same time, the reagent hole is rotated by the reagent disk, which can serve as an additive reminder for the experimenter. Avoid the failure of the experiment caused by the wrong sample; With the intelligent management terminal and the kit, the non-contact point-to-point data transmission can be realized within a distance of five centimeters, which solves many problems of the traditional kit production management method and improves the management. Efficiency and accuracy reduce management costs for manufacturing companies.

Claims

claims

[Claim 1] A reagent kit with cold preservation, alarm, wireless management and sample addition reminder functions, characterized by including a reagent kit body, the reagent kit body is provided with a liner, a number of reagent holes, a sound device and a control unit, small battery and near field communication NFC tag. The sound-generating device, the control unit and the battery are electrically connected.

[Claim 2] A test kit with cold preservation, alarm, wireless management and sample addition reminder functions according to claim 1, characterized in that the sound-generating device is a buzzer.

[Claim 3] A reagent kit with cold preservation, alarm, wireless management and sample addition reminder functions according to claim 1, characterized in that the reagent kit body has a double-layer structure, and the control unit and sound device and a small battery are arranged between the inner layer and the outer layer of the kit body.

[Claim 4] A reagent kit with cold preservation, alarm, wireless management and sample addition reminder functions according to claim 1, characterized in that the lining plate is provided with a pipette tip collection hole and a reagent rotation disk, and there is an arrow indicator light between the two. There is a certain distance between the lining plate and the opening of the box body, so that the lining plate and the box body form an open accommodation space.

; The reagent rotating disk is provided with a plurality of reagent holes and is connected to a power mechanism; A reagent tube is provided in the reagent hole;

A sensor is provided in the box body, the sensor is arranged corresponding to the bottom of the pipette tip collection hole, and the sensor controls the pipette tip by sensing the bottom of the pipette tip collection hole. The power mechanism drives the rotation of the reagent rotating disk and the color of the arrow indicator light.

[Claim 5] A test kit with cold preservation, alarm, wireless management and sample addition reminder functions according to claim 1, characterized in that the material of the arrow indicator light is LED, characterized in that, the arrow The indicator light is connected to the sensor and can respectively light up eight colors of light: red, orange, yellow, green, cyan, blue, purple and white as needed.

[Claim 6] A test kit with cold storage, alarm, wireless management and sample addition reminder functions according to claim 1, characterized in that the short-range wireless communication NFC tag is mainly composed of a data storage module and a data transmission module. It consists of module and NFC chip.

[Claim 7] A device with cooling, alarm, wireless management and sample adding reminder functions according to claim 1 A capable test kit, characterized in that the data storage module is electrically connected to the data transmission module, the data transmission module is electrically connected to the NFC chip and sends relevant data of the test kit to the intelligent management through wireless signals. on the terminal.