Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
  • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
  • A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
  • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
  • A61K31/235—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids having an aromatic ring attached to a carboxyl group

Definitions

• the present invention relates to an antibacterial agent for suppressing the growth of Vibrio parahaemolyticus and a method for suppressing a disease caused by infection with Vibrio parahaemolyticus of marine organisms.
• Early death syndrome is a disease in which Vibrio parahaemolyticus grows in the shrimp liver pancreas and necroses the liver pancreas.
• Patent Document 1 a technique using an antibacterial agent instead of an antibiotic is disclosed (for example, see Patent Document 1).
• the antibacterial agent described in Patent Document 1 is a composition containing catechins, which are natural substances, and it is relatively unlikely to remain in the shrimp body like antibiotics or adversely affect the surrounding environment.
• Patent Document 1 when the composition described in Patent Document 1 is used, it must be used in a large amount in order to obtain the same effect as an antibiotic. Therefore, there is a possibility that the water quality will be changed and contaminated.
• the antibacterial agent of the present invention is an antibacterial agent for suppressing the growth of Vibrio parahaemolyticus and is characterized by containing at least propyl gallate.
• antibacterial means that the growth of microorganisms is suppressed or the number of viable microorganisms decreases with the passage of time.
• the antibacterial agent containing at least propyl gallate can effectively suppress the growth of Vibrio parahaemolyticus.
• the method of the present invention is a method for suppressing diseases caused by Vibrio parahaemolyticus infection of marine organisms, characterized by administering at least one of propyl gallate to the breeding water.
• the growth of Vibrio parahaemolyticus in the breeding water can be suppressed by adding at least one of propyl gallate to the breeding water.
• the disease by Vibrio parahaemolyticus infection of a marine organism can be suppressed.
• Vibrio parahaemolyticus can be suppressed by using propyl gallate.
• the antibacterial agent according to one embodiment of the present invention is characterized by containing at least propyl gallate as an active ingredient.
• the form of the antibacterial agent is not particularly limited, and may be any shape such as solid or liquid.
• the antibacterial agent may be propyl gallate itself or may contain other components such as excipients in addition to propyl gallate.
• propyl gallate is a phenolic compound having a pyrogallol group, and is known as a food additive used as an antioxidant.
• the antibacterial agent of this embodiment preferably contains 0.02 mmol / L or more of propyl gallate, more preferably 0.03 mmol / L or more, and even more preferably 0.04 mmol / L or more. .
• the method of using the antibacterial agent is not particularly limited, and examples thereof include a method of adding to breeding water and a method of mixing with feed.
• a method of adding to breeding water propyl gallate is administered so that the concentration becomes 0.02 mmol / L or more.
• propyl gallate is difficult to dissolve in water, it is desirable to dissolve it in a small amount of ethanol before administering it to the breeding water.
• the antibacterial activity was measured as follows.
• test reagent was added to the liquid medium to a predetermined concentration, and Vibrio parahaemolyticus was inoculated therein to a concentration of 1.2 ⁇ 10 3 cfu / ml.
• the inoculated liquid medium was cultured at 35 ° C. for 18 hours or longer.
• the cultured liquid medium was applied to a TCBS medium and cultured at 35 ° C. for 18 hours or longer to form colonies.
• the final concentration of propyl gallate is 0.0125, 0.0250, 0.0500, 0.100, 50 ml of liquid medium (3.4% sodium chloride-added alkaline peptone water (pH 8.6)). The adjustment liquid was added so that it might become 0.200 mmol / L. Vibrio parahaemolyticus was inoculated to the medium adjusted to each concentration, and cultured at 35 ° C. for 24 hours.
• the cultured liquid medium was applied to a TCBS medium and cultured at 35 ° C. for 20 hours, and then the number of colonies was counted.
• Example 1 The same as Example 1 except that catechin (manufactured by Tokyo Chemical Industry Co., Ltd .: product name (+)-Catechin Hydrate) was used instead of propyl gallate.
• catechin manufactured by Tokyo Chemical Industry Co., Ltd .: product name (+)-Catechin Hydrate
• Example 1 is the same as Example 1 except that butylhydroxyanisole (manufactured by Kishida Chemical Co., Ltd .: product name butylhydroxyanisole) is used instead of propyl gallate.
• butylhydroxyanisole manufactured by Kishida Chemical Co., Ltd .: product name butylhydroxyanisole
• Example 3 The same as Example 1 except that chloramphenicol (manufactured by Tokyo Chemical Industry Co., Ltd .: product name Chloramphenicol) was used instead of propyl gallate.
• Example 1 is the same as Example 1 except that tetracycline (manufactured by Tokyo Chemical Industry Co., Ltd .: product name Tetracycline Hydrochloride) is used instead of propyl gallate.
• tetracycline manufactured by Tokyo Chemical Industry Co., Ltd .: product name Tetracycline Hydrochloride
• Example 5 The same as Example 1 except that nothing was added to the liquid medium.
• Example 2 The same as Example 1 except that pyrogallol (manufactured by Tokyo Chemical Industry Co., Ltd .: product name Pyrogallol) was used instead of propyl gallate.
• pyrogallol manufactured by Tokyo Chemical Industry Co., Ltd .: product name Pyrogallol
• Table 1 The results of antibacterial activity are shown in Table 1.
• the numerical values in the table are values of Log 10 cfu / ml.
• butylhydroxyanisole (Comparative Example 2), which is a food additive of the same antioxidant as propyl gallate, will be examined. Even when the concentration of butylhydroxyanisole was 0.200 mM / L, no antibacterial activity was observed compared to the case of no addition. In addition, even if it was a case where the density
• Chloramphenicol was found to have antibacterial activity at a lower concentration of 0.0125 mM / L than propyl gallate. Chloramphenicol has been used as an antibacterial agent in the past, and was most effective for the antibacterial activity of Vibrio parahaemolyticus in this study. Since chloramphenicol exhibited antibacterial activity even when the concentration was 0.0250 mM / L, no experiment was conducted for concentrations higher than that.
• Tetracycline exhibited antibacterial activity at a concentration of 0.0500 mM / L, but no antibacterial activity was observed at 0.0250 mM / L. From these, it can be seen that not all antibiotics are effective for the antibacterial activity of Vibrio parahaemolyticus. It can also be said that the minimum effective amount of tetracycline is between 0.0250 and 0.0500 mM.
• Pyrogallol is a phenol having a pyrogallol group like propyl gallate.
• pyrogallol was weaker in antibacterial activity than propyl gallate. Specifically, antibacterial activity was not observed unless 0.100 mM / L or more was added. In addition, even if it was a case where the density
• propyl gallate has a higher antibacterial activity even in a small amount than other comparative examples and reference examples although it has a weaker antibacterial activity than chloramphenicol. Moreover, since propyl gallate is a food additive, it is safer than antibiotics. In addition, propyl gallate is excellent in economic efficiency because of its low price.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Veterinary Medicine (AREA)
• Pest Control & Pesticides (AREA)
• Epidemiology (AREA)
• Medicinal Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
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• Agronomy & Crop Science (AREA)
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• Wood Science & Technology (AREA)
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• Environmental Sciences (AREA)
• Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
• Fodder In General (AREA)
• Agricultural Chemicals And Associated Chemicals (AREA)

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• 2015
  • 2015-10-09 JP JP2015200735A patent/JP6336424B2/ja active Active
• 2016
  • 2016-10-11 WO PCT/JP2016/080088 patent/WO2017061630A1/ja not_active Ceased
  • 2016-10-11 CN CN201680052993.4A patent/CN108024985A/zh active Pending

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