WO2017061630A1 - 抗菌剤および海洋生物の腸炎ビブリオの感染による疾病を抑制する方法 - Google Patents
抗菌剤および海洋生物の腸炎ビブリオの感染による疾病を抑制する方法 Download PDFInfo
- Publication number
- WO2017061630A1 WO2017061630A1 PCT/JP2016/080088 JP2016080088W WO2017061630A1 WO 2017061630 A1 WO2017061630 A1 WO 2017061630A1 JP 2016080088 W JP2016080088 W JP 2016080088W WO 2017061630 A1 WO2017061630 A1 WO 2017061630A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- propyl gallate
- vibrio parahaemolyticus
- antibacterial agent
- infection
- concentration
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
- A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
- A61K31/235—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids having an aromatic ring attached to a carboxyl group
Definitions
- the present invention relates to an antibacterial agent for suppressing the growth of Vibrio parahaemolyticus and a method for suppressing a disease caused by infection with Vibrio parahaemolyticus of marine organisms.
- Early death syndrome is a disease in which Vibrio parahaemolyticus grows in the shrimp liver pancreas and necroses the liver pancreas.
- Patent Document 1 a technique using an antibacterial agent instead of an antibiotic is disclosed (for example, see Patent Document 1).
- the antibacterial agent described in Patent Document 1 is a composition containing catechins, which are natural substances, and it is relatively unlikely to remain in the shrimp body like antibiotics or adversely affect the surrounding environment.
- Patent Document 1 when the composition described in Patent Document 1 is used, it must be used in a large amount in order to obtain the same effect as an antibiotic. Therefore, there is a possibility that the water quality will be changed and contaminated.
- the antibacterial agent of the present invention is an antibacterial agent for suppressing the growth of Vibrio parahaemolyticus and is characterized by containing at least propyl gallate.
- antibacterial means that the growth of microorganisms is suppressed or the number of viable microorganisms decreases with the passage of time.
- the antibacterial agent containing at least propyl gallate can effectively suppress the growth of Vibrio parahaemolyticus.
- the method of the present invention is a method for suppressing diseases caused by Vibrio parahaemolyticus infection of marine organisms, characterized by administering at least one of propyl gallate to the breeding water.
- the growth of Vibrio parahaemolyticus in the breeding water can be suppressed by adding at least one of propyl gallate to the breeding water.
- the disease by Vibrio parahaemolyticus infection of a marine organism can be suppressed.
- Vibrio parahaemolyticus can be suppressed by using propyl gallate.
- the antibacterial agent according to one embodiment of the present invention is characterized by containing at least propyl gallate as an active ingredient.
- the form of the antibacterial agent is not particularly limited, and may be any shape such as solid or liquid.
- the antibacterial agent may be propyl gallate itself or may contain other components such as excipients in addition to propyl gallate.
- propyl gallate is a phenolic compound having a pyrogallol group, and is known as a food additive used as an antioxidant.
- the antibacterial agent of this embodiment preferably contains 0.02 mmol / L or more of propyl gallate, more preferably 0.03 mmol / L or more, and even more preferably 0.04 mmol / L or more. .
- the method of using the antibacterial agent is not particularly limited, and examples thereof include a method of adding to breeding water and a method of mixing with feed.
- a method of adding to breeding water propyl gallate is administered so that the concentration becomes 0.02 mmol / L or more.
- propyl gallate is difficult to dissolve in water, it is desirable to dissolve it in a small amount of ethanol before administering it to the breeding water.
- the antibacterial activity was measured as follows.
- test reagent was added to the liquid medium to a predetermined concentration, and Vibrio parahaemolyticus was inoculated therein to a concentration of 1.2 ⁇ 10 3 cfu / ml.
- the inoculated liquid medium was cultured at 35 ° C. for 18 hours or longer.
- the cultured liquid medium was applied to a TCBS medium and cultured at 35 ° C. for 18 hours or longer to form colonies.
- the final concentration of propyl gallate is 0.0125, 0.0250, 0.0500, 0.100, 50 ml of liquid medium (3.4% sodium chloride-added alkaline peptone water (pH 8.6)). The adjustment liquid was added so that it might become 0.200 mmol / L. Vibrio parahaemolyticus was inoculated to the medium adjusted to each concentration, and cultured at 35 ° C. for 24 hours.
- the cultured liquid medium was applied to a TCBS medium and cultured at 35 ° C. for 20 hours, and then the number of colonies was counted.
- Example 1 The same as Example 1 except that catechin (manufactured by Tokyo Chemical Industry Co., Ltd .: product name (+)-Catechin Hydrate) was used instead of propyl gallate.
- catechin manufactured by Tokyo Chemical Industry Co., Ltd .: product name (+)-Catechin Hydrate
- Example 1 is the same as Example 1 except that butylhydroxyanisole (manufactured by Kishida Chemical Co., Ltd .: product name butylhydroxyanisole) is used instead of propyl gallate.
- butylhydroxyanisole manufactured by Kishida Chemical Co., Ltd .: product name butylhydroxyanisole
- Example 3 The same as Example 1 except that chloramphenicol (manufactured by Tokyo Chemical Industry Co., Ltd .: product name Chloramphenicol) was used instead of propyl gallate.
- Example 1 is the same as Example 1 except that tetracycline (manufactured by Tokyo Chemical Industry Co., Ltd .: product name Tetracycline Hydrochloride) is used instead of propyl gallate.
- tetracycline manufactured by Tokyo Chemical Industry Co., Ltd .: product name Tetracycline Hydrochloride
- Example 5 The same as Example 1 except that nothing was added to the liquid medium.
- Example 2 The same as Example 1 except that pyrogallol (manufactured by Tokyo Chemical Industry Co., Ltd .: product name Pyrogallol) was used instead of propyl gallate.
- pyrogallol manufactured by Tokyo Chemical Industry Co., Ltd .: product name Pyrogallol
- Table 1 The results of antibacterial activity are shown in Table 1.
- the numerical values in the table are values of Log 10 cfu / ml.
- butylhydroxyanisole (Comparative Example 2), which is a food additive of the same antioxidant as propyl gallate, will be examined. Even when the concentration of butylhydroxyanisole was 0.200 mM / L, no antibacterial activity was observed compared to the case of no addition. In addition, even if it was a case where the density
- Chloramphenicol was found to have antibacterial activity at a lower concentration of 0.0125 mM / L than propyl gallate. Chloramphenicol has been used as an antibacterial agent in the past, and was most effective for the antibacterial activity of Vibrio parahaemolyticus in this study. Since chloramphenicol exhibited antibacterial activity even when the concentration was 0.0250 mM / L, no experiment was conducted for concentrations higher than that.
- Tetracycline exhibited antibacterial activity at a concentration of 0.0500 mM / L, but no antibacterial activity was observed at 0.0250 mM / L. From these, it can be seen that not all antibiotics are effective for the antibacterial activity of Vibrio parahaemolyticus. It can also be said that the minimum effective amount of tetracycline is between 0.0250 and 0.0500 mM.
- Pyrogallol is a phenol having a pyrogallol group like propyl gallate.
- pyrogallol was weaker in antibacterial activity than propyl gallate. Specifically, antibacterial activity was not observed unless 0.100 mM / L or more was added. In addition, even if it was a case where the density
- propyl gallate has a higher antibacterial activity even in a small amount than other comparative examples and reference examples although it has a weaker antibacterial activity than chloramphenicol. Moreover, since propyl gallate is a food additive, it is safer than antibiotics. In addition, propyl gallate is excellent in economic efficiency because of its low price.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Pest Control & Pesticides (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Emergency Medicine (AREA)
- Agronomy & Crop Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Fodder In General (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
抗菌活性の測定は、次のようにして行った。
没食子酸プロピル(東京化成工業株式会社製:商品名Propyl Gallate)をエタノールで溶解した後、PBS(-)を用いて濃度2mmol/Lの調整液を作成した。
没食子酸プロピルの代わりに、カテキン(東京化成工業株式会社製:製品名(+)-Catechin Hydrate)を用いたこと以外は、実施例1と同じである。
没食子酸プロピルの代わりに、ブチルヒドロキシアニソール(キシダ化学株式会社製:製品名ブチルヒドロキシアニソール)を用いたこと以外は、実施例1と同じである。
没食子酸プロピルの代わりに、クロラムフェニコール(東京化成工業株式会社製:製品名Chloramphenicol)を用いたこと以外は、実施例1と同じである。
没食子酸プロピルの代わりに、テトラサイクリン(東京化成工業株式会社製:製品名Tetracycline Hydrochloride)を用いたこと以外は、実施例1と同じである。
液体培地に何も添加しなかったこと以外は、実施例1と同じである。
没食子酸プロピルの代わりに、ピロガロール(東京化成工業株式会社製:製品名Pyrogallol)を用いたこと以外は、実施例1と同じである。
Claims (2)
- 腸炎ビブリオ(Vibrio parahaemolyticus)の増殖を抑制するための抗菌剤であって、少なくとも没食子酸プロピルを含有している抗菌剤。
- 海洋生物の腸炎ビブリオ感染による疾病を抑制するための方法であって、没食子酸プロピルの少なくともいずれかを飼育水に投与することを特徴とする方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201680052993.4A CN108024985A (zh) | 2015-10-09 | 2016-10-11 | 抗微生物剂和抑制海洋生物副溶血弧菌感染引起的疾病的方法 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015-200735 | 2015-10-09 | ||
JP2015200735A JP6336424B2 (ja) | 2015-10-09 | 2015-10-09 | 抗菌剤および海洋生物の腸炎ビブリオの感染による疾病を抑制する方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2017061630A1 true WO2017061630A1 (ja) | 2017-04-13 |
Family
ID=58487830
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2016/080088 WO2017061630A1 (ja) | 2015-10-09 | 2016-10-11 | 抗菌剤および海洋生物の腸炎ビブリオの感染による疾病を抑制する方法 |
Country Status (3)
Country | Link |
---|---|
JP (1) | JP6336424B2 (ja) |
CN (1) | CN108024985A (ja) |
WO (1) | WO2017061630A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115501214A (zh) * | 2022-10-14 | 2022-12-23 | 湖北省农业科学院畜牧兽医研究所 | 焦棓酸与磺胺异恶唑组合物及其在抑制猪链球菌2型中的应用 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112450355A (zh) * | 2020-11-23 | 2021-03-09 | 大连工业大学 | 一种抗菌肽Cm-CATH2抑制海产品中副溶血性弧菌的应用及用途 |
CN115400105B (zh) * | 2022-10-12 | 2023-08-22 | 湖北省农业科学院畜牧兽医研究所 | 焦棓酸在制备抗马链球菌兽疫亚种药物中的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1143441A (ja) * | 1997-07-29 | 1999-02-16 | Ichimaru Pharcos Co Ltd | 魚介類の疾病予防治療剤 |
JP2004315386A (ja) * | 2003-04-14 | 2004-11-11 | Hayashibara Takeshi | 抗微生物剤 |
JP2006306836A (ja) * | 2005-01-11 | 2006-11-09 | Microbiotech:Kk | 抗ウイルス・抗炎症作用薬剤組成物 |
WO2008081901A1 (ja) * | 2006-12-28 | 2008-07-10 | Microbiotech Inc. | アルキルガレート薬剤組成物 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104872416A (zh) * | 2015-05-05 | 2015-09-02 | 中国林业科学研究院林产化学工业研究所 | 五倍子提取物的应用 |
-
2015
- 2015-10-09 JP JP2015200735A patent/JP6336424B2/ja active Active
-
2016
- 2016-10-11 WO PCT/JP2016/080088 patent/WO2017061630A1/ja active Application Filing
- 2016-10-11 CN CN201680052993.4A patent/CN108024985A/zh active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH1143441A (ja) * | 1997-07-29 | 1999-02-16 | Ichimaru Pharcos Co Ltd | 魚介類の疾病予防治療剤 |
JP2004315386A (ja) * | 2003-04-14 | 2004-11-11 | Hayashibara Takeshi | 抗微生物剤 |
JP2006306836A (ja) * | 2005-01-11 | 2006-11-09 | Microbiotech:Kk | 抗ウイルス・抗炎症作用薬剤組成物 |
WO2008081901A1 (ja) * | 2006-12-28 | 2008-07-10 | Microbiotech Inc. | アルキルガレート薬剤組成物 |
Non-Patent Citations (1)
Title |
---|
YAMANAKA FUMI ET AL.: "Antibacterial Effects of Guava Tannnins and Related Polyphenols on Vibrio and Aeromonas Species", NATURAL PRODUCT COMMUNICATIONS, vol. 3, no. 5, 2008, pages 711 - 720 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115501214A (zh) * | 2022-10-14 | 2022-12-23 | 湖北省农业科学院畜牧兽医研究所 | 焦棓酸与磺胺异恶唑组合物及其在抑制猪链球菌2型中的应用 |
CN115501214B (zh) * | 2022-10-14 | 2023-09-22 | 湖北省农业科学院畜牧兽医研究所 | 焦棓酸与磺胺异恶唑组合物及其在抑制猪链球菌2型中的应用 |
Also Published As
Publication number | Publication date |
---|---|
JP2017071583A (ja) | 2017-04-13 |
CN108024985A (zh) | 2018-05-11 |
JP6336424B2 (ja) | 2018-06-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5711846B1 (ja) | 養殖用水中の水カビ防除方法 | |
TWI488625B (zh) | 殺真菌劑用於治療魚類真菌病之用途(二) | |
JP6336424B2 (ja) | 抗菌剤および海洋生物の腸炎ビブリオの感染による疾病を抑制する方法 | |
JP4121045B2 (ja) | 殺微生物組成物及び微生物の制御方法 | |
JP2009132702A (ja) | 抗菌剤水溶液及びその製造方法 | |
CN108883095B (zh) | 鳞介类的微孢子虫的防除用组合物、及使用了该组合物的鳞介类的微孢子虫的防除方法 | |
JP2006206558A (ja) | 食中毒予防用抗菌性組成物 | |
CN103891772A (zh) | 一种复方消毒杀菌剂 | |
KR100845424B1 (ko) | 양식어류의 질병 예방 및 치료를 위한 조성물 | |
EP2681996A1 (en) | Biocidal compositions | |
CN104382939A (zh) | 一种用于水产养殖的杀轮虫药剂 | |
JP6579959B2 (ja) | 細菌性皮膚病用殺菌組成物 | |
CN110917057A (zh) | 一种复配防腐剂试剂及其制备方法及其在化妆品中的应用 | |
JPWO2016068230A1 (ja) | バイオフィルム形成抑制剤および/または除去剤、ならびにバイオフィルム形成抑制方法および/または除去方法 | |
CN101461382A (zh) | 复方高效禽流感消毒液 | |
CN112203514B (zh) | 消毒剂组合物 | |
CN1514724A (zh) | 用于养殖鱼的杀寄生虫药 | |
JP2010235509A (ja) | 微生物防除剤、及び、微生物防除方法 | |
Liao et al. | The use of chemicals in aquaculture in Taiwan, Province of China | |
KR100751520B1 (ko) | 어류양식장용 소독제의 제조방법 | |
CN114748453A (zh) | 一种用于水产养殖动物疾病防治的杀菌组合物及其应用 | |
CN112494514A (zh) | 一种杀菌消毒剂及其制备方法 | |
JP5547833B2 (ja) | 微生物防除剤、及び、微生物防除方法 | |
JP2013538868A5 (ja) | ||
JP2010024206A (ja) | 工業用防腐防かび剤 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 16853781 Country of ref document: EP Kind code of ref document: A1 |
|
DPE1 | Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101) | ||
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112018005610 Country of ref document: BR |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 112018005610 Country of ref document: BR Kind code of ref document: A2 Effective date: 20180321 |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 16853781 Country of ref document: EP Kind code of ref document: A1 |