WO2016209047A1 - Cordyceps cultivés à l'aide de hippocampes, et procédé de culture de cordyceps - Google Patents

Cordyceps cultivés à l'aide de hippocampes, et procédé de culture de cordyceps Download PDF

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Publication number
WO2016209047A1
WO2016209047A1 PCT/KR2016/006805 KR2016006805W WO2016209047A1 WO 2016209047 A1 WO2016209047 A1 WO 2016209047A1 KR 2016006805 W KR2016006805 W KR 2016006805W WO 2016209047 A1 WO2016209047 A1 WO 2016209047A1
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Prior art keywords
hippocampus
cordyceps
seahorse
cordyceps sinensis
medium
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PCT/KR2016/006805
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English (en)
Korean (ko)
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이충하
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스마테크 주식회사
이충하
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Publication of WO2016209047A1 publication Critical patent/WO2016209047A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/20—Culture media, e.g. compost
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers

Definitions

  • the present invention relates to Cordyceps sinensis cultivated using seahorse and its cultivation method.
  • Cordyceps sinensis In winter, insects are parasitic in winter, but in summer they form mushrooms in the body of insects, and the name of this is Cordyceps sinensis, which invades insects, kills insects, and then forms a locus from the host. However, even if the insects invade and kill the insects, there is also a type that forms a conidia, an asexual reproductive organ, on the surface of the host without forming a chair. Most of the cordyceps that form the locus are Cordyceps genus Cordyceps, Podonectria, Torrubiella belonging to the genus Bacillus Bacillus, etc. Cordyceps sinensis is widely distributed in Korea, China and Japan.
  • Insects which are the hosts of Cordyceps sinensis, are parasitic to spiders in addition to Lepidoptera (Cordyceps militaris), Cicada (C. sobolifera), and Lumberjack (C. sphecocephala) and other coleopteran and locust trees.
  • Lepidoptera Cordyceps militaris
  • Cicada Cicada
  • Lumberjack C. sphecocephala
  • beetle cordyceps hemipteran cordyceps
  • bee cordyceps hemipteran cordyceps
  • bee cordyceps bee cordyceps
  • snowflake cordyceps and caterpillar cordyceps parasitic from bat moth larva.
  • the medicinal effect has been recognized bat moth cordyceps, but the cordyceps is collected only in the alpine region of 4000m or more above sea level, there is a problem that artificial cultivation is difficult.
  • bee cordyceps, scarab beetle and stink bug cordyceps are not suitable for edible use because their host is an aversive insect. Therefore, artificial cultivation has been studied only for pupa cordyceps and snowflower cordyceps based on silkworm or silkworm pupa.
  • cordyceps fungi When the cordyceps fungi are permanently formed, they form molecular spores or follicle spores and attach them to the joints and digestive organs of living insects, forming hyphae while ingesting nutrients in the insect body, thereby killing insects. In the summer, fruiting bodies are formed using the nutrients of its host insect.
  • Cordyceps sinensis generally consists of water, carbohydrates, fats, ash and crude proteins, and the protein contains about 18 essential amino acids.
  • Cordyceps sinensis contains special ingredients such as Choong-poly polysaccharide and Chungchoic acid, and is known as a therapeutic agent for anemia, fatigue, high blood pressure, pulmonary tuberculosis and asthma.
  • Cordyceps sinensis contains ingredients that enhance immune function, sexual stimulants and anti-cancer ingredients, and research is being actively conducted, and it is excellent as an antidote for drug addicts and has parasitic properties in insects. As a result, it is very useful as a biopesticide that can provide effective pest control and prevent environmental pollution.
  • the artificial cultivation method of Cordyceps sinensis known to date is based on the fact that Cordyceps sinensis forms fruiting bodies based on insects, and there is a method of producing fruiting bodies by inoculating pupa cordyceps and snowflower cordyceps on silkworm larvae or silkworm pupae.
  • the present invention discloses a method of culturing fruiting bodies by inoculating Cordyceps sinensis liquid seedlings so that the whole surface of the medium can be grown in a state in which the pupa is added to the grain medium.
  • cordyceps cultivation method (application number 10-1999-025540) using soybean fiber foil
  • cordyceps larvae based on beetle larvae and its production method (application number 10-1999-026782) and the like are known.
  • Seahorses live primarily in shallow waters in subtropical seas that are not contaminated with fish.
  • the hippocampus has been prescribed in China and other Chinese culture countries as a viable drug for intractable diseases as well as energetic agents.
  • According to international trade data at least 20 million dried seahorses were distributed worldwide in 1995 alone, presumably used as traditional Chinese medicines or derivatives thereof.
  • the present invention has been made to solve the above-mentioned problems, to provide a Cordyceps sinensis and its cultivation method based on the hippocampus. More specifically, in order to meet the situation that needs to develop a variety of host animals for the cultivation of Cordyceps sinensis excellent in terms of ingredients and effects, Cordyceps cultivation method using seahorses as a substrate to solve the problems of ease of cultivation, productivity, supply of raw materials, etc. To provide.
  • the present invention culture step for culturing the Cordyceps fungi spawn in the spawn culture medium; A medium forming step of forming a hippocampal medium based on seahorse; An inoculation step of inoculating the cultured Cordyceps sinensis seed in the formed hippocampus medium; And culturing the inoculated Cordyceps spp. In the hippocampal medium to form a mycelium and a fruiting body.
  • seahorse provides a cultivation method of seahorse cordyceps including cultured seahorse.
  • the seahorse also provides a cultivation method of hippocampus Cordyceps, including big-belly seahorse or pot-bellied seahorse.
  • the medium forming step provides a cultivation method of hippocampus Cordyceps sinensis which is a step of forming a hippocampus medium based on the hippocampus dried for 5 to 10 hours at 30 to 60 °C discharged the excreta.
  • the medium forming step further includes a desalting step of removing the salts of the hippocampus, wherein the desalting step is a mixture of brine having a salinity equivalent to that of the hippocampus and the dried hippocampus under alcohol. It provides a cultivation method of hippocampus Cordyceps comprising the step of.
  • the desalting step provides a cultivation method of hippocampus Cordyceps, further comprising the step of stationary treatment for 15 to 20 hours and the filtration treatment of the hippocampus after the mixing treatment step.
  • the culture step provides a cultivation method of hippocampus Cordyceps sinensis which is a step of inoculating the liquid seed or solid seedling of the Cordyceps sinensis in the aseptic culture medium.
  • the inoculation step is a sterilization of the hippocampus medium and then dissecting the outer skin of the hippocampus to provide a cultivation method of the hippocampus Cordyceps, which is a step of inoculating the cultured Cordyceps spp.
  • the growth step is incubated with mycelium at the conditions of temperature 23 to 27 °C, humidity 65 to 75%, the step of growing after fruiting the fruiting body by irradiation with light at the temperature of 15 to 20 °C, humidity 80 to 95% Provides a method of cultivating seahorse cordyceps.
  • the present invention provides a hippocampus Cordyceps sinensis comprising the nutrients of the seahorse (seahorse).
  • the nutrient is selected from the group consisting of taurine (taurine), arginine (arginine), aspartic acid (aspartic acid), alanine (alanine), glycine (glycine), proline (proline) and glutamic acid (glutamic acid)
  • taurine taurine
  • arginine arginine
  • aspartic acid aspartic acid
  • alanine alanine
  • glycine glycine
  • proline proline
  • glutamic acid glutamic acid
  • the nutrients include calcium (calcium), magnesium (magnesium), potassium (potassium), sodium (sodium), iron (iron), zinc (zinc), manganese (manganese), copper (copper) and chromium (chromium) It provides a hippocampus Cordyceps sinensis comprising any one or more minerals selected from the group consisting of.
  • the present invention provides a food comprising the hippocampus Cordyceps sinensis as one component.
  • hippocampus Cordyceps sinensis prepared by the present invention as a herbal medicine can be used for the treatment of diseases such as asthma, heart disease, fracture.
  • Method of cultivating the hippocampus Cordyceps sinensis of the present invention is a culture step (S1) of cultivating the species of Cordyceps sinensis, a medium formation step (S2) to form a hippocampus medium based on the hippocampus, the inoculation step of inoculating Cordyceps fungi cultured in the hippocampus medium (S3), and the growth step (S4) of culturing the inoculated Cordyceps fungi spawn to form mycelium and fruiting body.
  • the culturing step (S1) is a step of inoculating a liquid spawn or a solid spawn of Cordyceps sinensis in a spawn culture medium in a sterile state, and as a Cordyceps spawn seed, cultured in a liquid culture solution using potato or soy flour.
  • General solid spawn grown in a solid medium such as liquid spawn or brown rice can be used.
  • the spawn culture medium any medium such as a liquid medium or a solid medium, a natural medium or a synthetic medium can be used, and can be cultured Cordyceps fungi using the hippocampal medium prepared in the medium forming step (S2) described later.
  • Cordyceps fungi can be applied without restriction, such as Paecilomyces japonica bacteria or Militaris cordyceps fungi (Cordyceps militaris).
  • Sterilization is preferably subjected to secondary high temperature sterilization at 100 to 120 ° C. after the first low temperature sterilization at 80 to 100 ° C. After cooling the sterilized medium, the fungus is inoculated into the medium.
  • Medium formation step (S2) is a step of forming a culture medium based on the hippocampus, by stopping the feeding for at least 24 hours to discharge the excretion of the hippocampus to form a hippocampus medium do.
  • Cultured seahorses may be used as the hippocampus, and preferably, big-belly seahorses or pot-bellid seahorses are used.
  • the present invention is not limited thereto, and any type of seahorse, such as a Great Seahorse (H. kelloggi), a Spotted seahorse (H. kuda), or a Longnose seahorse (H. trimaculatus), may be used without limitation. .
  • the hippocampus to be used as the medium stops feeding at least 24 hours, preferably 24 to 48 hours, to excrete feces. It is preferable to dry the hippocampus from which excreta was discharged at 30-60 degreeC for 5 to 10 hours. However, depending on the condition of the hippocampus, the desired culture conditions, and the type of Cordyceps fungus, the drying temperature or drying time can be controlled.
  • the dried hippocampus can be used as a medium by entering a glass bottle, a plastic bottle, a bag, or the like or as a medium itself.
  • Medium formation step (S2) may further include a desalination step (S201) for adjusting the salinity (salinity) of the hippocampus or a dehydration step (S202) for adjusting the water content of the hippocampus.
  • a desalination step (S201) for adjusting the salinity (salinity) of the hippocampus
  • a dehydration step (S202) for adjusting the water content of the hippocampus.
  • it may include both the desalting step (S201) and the dehydration step (S202), in which case it is preferable to perform the desalting step (S201) first.
  • the salinity of the hippocampus is adjusted to a level suitable for cultivating Cordyceps sinensis.
  • a brine having a salinity equivalent to that of the hippocampus and a hippocampus are mixed under alcohol, and after stationary for 15 to 20 hours, the hippocampus is filtered to control salinity. Getting a hippocampus.
  • the brine used in the desalting step S201 includes seawater or saline and includes a solution containing salts such as calcium chloride.
  • the salinity of brine is equivalent to that of the hippocampus used as a medium.
  • the desalting step (S201) is a step of precipitating salts by mixing under alcohol to lower the solubility of salts. It is preferable to use ethyl alcohol or pretanol A among alcohols.
  • the desalting step (S201) 80 to 120 parts by mass of hippocampus is mixed with respect to 100 parts by mass of brine, and the stationary treatment is performed under 500 to 1000 parts by mass of alcohol.
  • the salted seahorse is filtered off and washed to obtain a salted seahorse.
  • the moisture content of the hippocampus is adjusted to a level suitable for cultivating Cordyceps sinensis. It is preferable to dry the hippocampus or the salinity-controlled hippocampus by evaporation of moisture in the hippocampus under 20 to 40 ° C. If the water content is high, the fruiting body germinates well, but the mycelium is not well cultured. If the water content is low, the mycelium is well cultured but the fruiting body does not germinate. Therefore, there is a need for a process for controlling the water content of the hippocampus to an appropriate level.
  • the drying temperature or drying time may be adjusted depending on the condition of the hippocampus, the desired culture conditions, and the type of Cordyceps inoculated.
  • the hippocampus alone, ie, 100% hippocampus may be composed of a medium or a hippocampus mixed medium in which other media components are additionally mixed.
  • Inoculation step (S3) is a step of inoculating the cultured Cordyceps spp.
  • a general liquid spawn cultured in a liquid medium using potato or soy flour, or a general solid spawn cultured in a solid medium such as brown rice can be used, and as described above, the seed spawn was cultured in a hippocampus medium according to the present invention. You can also use
  • Growth step (S4) is a step of culturing the inoculated Cordyceps fungi spawn to form a mycelium and fruiting body, it comprises a step of culturing with Cordyceps fungus mycelium in a culture room of the appropriate conditions.
  • the culture conditions of Cordyceps mycelium are made at a specific culture temperature and a specific room humidity, so that the seeds are spread evenly in the medium to complete the culture of the mycelium. In general, when incubated for 8 to 10 days at a temperature of 23 to 27 ° C. and a humidity of 65 to 75%, the spawn is spread evenly in the medium to culture the mycelia.
  • the growth step (S4) includes a step of growing the Cordyceps mycelium cultured in the above process into Cordyceps fruiting body under appropriate conditions. Growth conditions of Cordyceps sinensis are made at a certain temperature and humidity. Typically, the fruiting body comes out when irradiated with light of 100 to 200lx at a temperature of 15 to 20 °C, humidity 80 to 95%, the fruiting body grows when the light of 100 to 400lx on the fruiting body produced under the same conditions . In the case of Militaris Cordyceps sinensis, the fruiting body comes out when the light of about 800 to 1000lx for about 3 days, the fruiting body grows when 100 to 400lx light is irradiated on the fruiting body.
  • Hippocampus Cordyceps sinensis cultivated by the cultivation method according to an embodiment of the present invention includes a nutrient of seahorse (seahorse) used as a host.
  • the hippocampus Cordyceps sinensis according to an embodiment of the present invention is cultivated based on a bigbelly seahorse or pot-bellid seahorse, and includes nutrients of a bigbelly seahorse or a potbelly seahorse. do.
  • any type of seahorse such as Great seahorse (H. kelloggi), Spotted seahorse / H. Kuda, or Longnose seahorse / H. Trimaculatus, may be included without limitation.
  • Major nutrients included include arginine, aspartic acid, alanine, glycine, proline and glutamic acid, including taurine, which has high medicinal value.
  • arginine aspartic acid
  • alanine alanine
  • proline alanine
  • glutamic acid including taurine
  • taurine which has high medicinal value.
  • a food comprising a hippocampus Cordyceps sinensis according to one embodiment of the present invention as one component.
  • Cordyceps sinensis of the present invention can be freeze-dried according to a conventional method for commercialization, or can be used as a water extract or liquor.
  • Cordyceps sinensis containing nutrients of the hippocampus is used as a therapeutic agent for sexual dysfunction, renal dysfunction, and excessive cholesterol. It also acts on the liver and kidney to promote blood circulation, antithrombotic and anti-aging effect.
  • Cordyceps sinensis As a seed for cultivation of Cordyceps sinensis, a liquid culture of the seedlings of Cordyceps militaris was used. Cordyceps sinensis inoculum was used to culture the Cordyceps fungi spawn at 22-30 °C for 5 days using potato sugar medium (PD broth).
  • PD broth potato sugar medium
  • the hippocampus used the Big Valley seahorse farmed in Jeju Island. Feeding the hippocampus was stopped for 36 hours to discharge the excreta and dried for 6 hours at 40 °C.
  • a hippocampal medium As a hippocampal medium, a medium having a composition of 100 g of dried seahorse, 30 g of water, and 2 g of sugar was formed. The medium was filled in a plastic container (about 850 ml), and sterilized for 120 minutes at a temperature of 121 ° C. and 1.5 atm.
  • the excreta was discharged and dried at 30 ° C. for 5 hours. Then, 100 g of brine with 13% salinity and 100 g of dried seahorse were added to 800 g of pretanol A, mixed and left for 15 hours. The hippocampus was filtered off and evaporated at room temperature to give dried hippocampus.
  • Hippocampus Cordyceps was grown in the same manner as in Example 1 except that the desalted and dried hippocampus was used as the base.
  • Cultivation of mushrooms by the method of the above example was harvested when the diameter of the fruiting body was 7cm, and the number of culture days and growth days until harvesting was measured and shown in Table 1 below.
  • the culture days are days required for mycelia to grow from the spawn inoculation to the bottom of the bottle, and the growth days are the days taken from the fruiting body to the harvest date.
  • Example 2 when the desalted hippocampus was used as a medium (Example 2), the culture days and growth days were shortened as compared with the case of using the dried hippocampus (Example 1). .
  • Example 1 Example 2 Unit Protein 52.8 64.1 % Saccharide 12.5 15.1 g / 100g Invert sugar 4.5 5.5 g / 100g
  • Example 2 As shown in Table 2, in the case of Cordyceps sinensis grown on the desalted, dried seahorse of Example 2, 64.1% protein, 15.1 g / 100g of saccharides, the case of Cordyceps sinensis grown on the dried hippocampus of Example 1 (Protein 52.8%, sugar 12.5 g / 100g).
  • Example 1 Ca 39.48 32.9 Mg 84.1 96.9 K 181.8 196.95 Na 100.92 109.33 Fe 23.28 25.22 Zn 7.56 8.19 Mn 1.82 1.68 Cu 1.8 1.8 Cr 1.4 1.5
  • Example 1 arginine 0.945 1.044 aspartic acid 1.222 1.664 alanine 0.851 1.090 glycine 0.522 0.596 taurine 0.543 0.692 proline 0.604 0.669 glutamic acid 1.575 1.606 serine 6.262 7.360 histidine 0.274 0.357 threonine 0.386 0.377 methionine 0.134 0.086 isoleucine 0.228 0.131 Total 9.710 10.566
  • the amino acid content was detected and identified 12 amino acids, including taurine (taurine).
  • the total amino acid content of the hippocampus Cordyceps sinensis according to the method of Example was 9.710g and 10.566g per 100g, respectively, and taurine contained 0.712g / 100g and 0.878g / 100g.

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract

L'invention concerne des cordyceps contenant des éléments nutritionnels de hippocampes en ayant été cultivés à l'aide de hippocampes comme hôtes, et un procédé de culture de cordyceps. En conséquence, la présente invention concerne des cordyceps de cheval de mer contenant de nouveaux éléments nutritionnels ne pouvant pas être obtenus à partir des cordyceps classiques et, en fournissant un aliment contenant les cordyceps de cheval de mer comme ingrédient, la sécrétion d'hormones sexuelles peut être rendue active et des effets antivieillissement et antithrombotique peuvent être obtenus.
PCT/KR2016/006805 2015-06-24 2016-06-24 Cordyceps cultivés à l'aide de hippocampes, et procédé de culture de cordyceps WO2016209047A1 (fr)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN107624500A (zh) * 2017-10-10 2018-01-26 广西金诚双丰农牧科技有限公司 利用竹鼠粪便栽培平菇的方法

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KR102483164B1 (ko) * 2020-10-12 2022-12-29 임영길 고함량 코디세핀을 함유하는 배스 및/또는 블루길을 이용한 동충하초 및 이의 재배방법

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KR19990076445A (ko) * 1998-03-26 1999-10-15 김동원 동물성배지를 이용한 동충하초 재배방법
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107624500A (zh) * 2017-10-10 2018-01-26 广西金诚双丰农牧科技有限公司 利用竹鼠粪便栽培平菇的方法

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