WO2016209047A1 - Cordyceps cultivated using sea horses and method for cultivating the cordyceps - Google Patents

Abstract

Provided are cordyceps containing nutritional components of sea horses by having been cultivated using sea horses as hosts, and a method for cultivating the cordyceps. Accordingly, provided are sea horse cordyceps containing novel nutritional components not obtainable from the conventional cordyceps, and by providing food containing the sea horse cordyceps as an ingredient, the secretion of sexual hormones can be made active and anti-aging and anti-thrombotic effects can be provided.

Description

Cordyceps sinensis cultivated using seahorse and its cultivation method

The present invention relates to Cordyceps sinensis cultivated using seahorse and its cultivation method.

In winter, insects are parasitic in winter, but in summer they form mushrooms in the body of insects, and the name of this is Cordyceps sinensis, which invades insects, kills insects, and then forms a locus from the host. However, even if the insects invade and kill the insects, there is also a type that forms a conidia, an asexual reproductive organ, on the surface of the host without forming a chair. Most of the cordyceps that form the locus are Cordyceps genus Cordyceps, Podonectria, Torrubiella belonging to the genus Bacillus Bacillus, etc. Cordyceps sinensis is widely distributed in Korea, China and Japan.

Insects, which are the hosts of Cordyceps sinensis, are parasitic to spiders in addition to Lepidoptera (Cordyceps militaris), Cicada (C. sobolifera), and Lumberjack (C. sphecocephala) and other coleopteran and locust trees. have. For example, beetle cordyceps, hemipteran cordyceps, bee cordyceps, snowflake cordyceps, and caterpillar cordyceps parasitic from bat moth larva.

Among them, the medicinal effect has been recognized bat moth cordyceps, but the cordyceps is collected only in the alpine region of 4000m or more above sea level, there is a problem that artificial cultivation is difficult. In addition, bee cordyceps, scarab beetle and stink bug cordyceps are not suitable for edible use because their host is an aversive insect. Therefore, artificial cultivation has been studied only for pupa cordyceps and snowflower cordyceps based on silkworm or silkworm pupa.

When the cordyceps fungi are permanently formed, they form molecular spores or follicle spores and attach them to the joints and digestive organs of living insects, forming hyphae while ingesting nutrients in the insect body, thereby killing insects. In the summer, fruiting bodies are formed using the nutrients of its host insect.

Cordyceps sinensis generally consists of water, carbohydrates, fats, ash and crude proteins, and the protein contains about 18 essential amino acids. In addition, Cordyceps sinensis contains special ingredients such as Choong-poly polysaccharide and Chungchoic acid, and is known as a therapeutic agent for anemia, fatigue, high blood pressure, pulmonary tuberculosis and asthma.

In addition, it is proved that Cordyceps sinensis contains ingredients that enhance immune function, sexual stimulants and anti-cancer ingredients, and research is being actively conducted, and it is excellent as an antidote for drug addicts and has parasitic properties in insects. As a result, it is very useful as a biopesticide that can provide effective pest control and prevent environmental pollution.

In spite of such excellent effect, it is very difficult to collect Cordyceps fruiting body grown in a natural state, so artificial cultivation technology for stably supplying Cordyceps fruiting body is required.

The artificial cultivation method of Cordyceps sinensis known to date is based on the fact that Cordyceps sinensis forms fruiting bodies based on insects, and there is a method of producing fruiting bodies by inoculating pupa cordyceps and snowflower cordyceps on silkworm larvae or silkworm pupae.

As a prior art, there is Korea Patent Publication No. 1998-023907. In the present invention, a method of culturing fruiting bodies by inoculating Cordyceps fungi to silkworm 4-5 larvae or pupa.

Another prior art is Korean Laid-Open Patent Publication No. 1998-033558. The present invention discloses a method of culturing fruiting bodies by inoculating Cordyceps sinensis liquid seedlings so that the whole surface of the medium can be grown in a state in which the pupa is added to the grain medium.

In addition, cordyceps cultivation method (application number 10-1999-025540) using soybean fiber foil, cordyceps larvae based on beetle larvae and its production method (application number 10-1999-026782) and the like are known.

Seahorses, on the other hand, live primarily in shallow waters in subtropical seas that are not contaminated with fish. The hippocampus has been prescribed in China and other Chinese culture countries as a viable drug for intractable diseases as well as energetic agents. According to international trade data, at least 20 million dried seahorses were distributed worldwide in 1995 alone, presumably used as traditional Chinese medicines or derivatives thereof.

However, a technique for culturing Cordyceps sinensis based on the hippocampus has not been known.

The present invention has been made to solve the above-mentioned problems, to provide a Cordyceps sinensis and its cultivation method based on the hippocampus. More specifically, in order to meet the situation that needs to develop a variety of host animals for the cultivation of Cordyceps sinensis excellent in terms of ingredients and effects, Cordyceps cultivation method using seahorses as a substrate to solve the problems of ease of cultivation, productivity, supply of raw materials, etc. To provide.

However, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned will be clearly understood by those skilled in the art from the following description.

The present invention culture step for culturing the Cordyceps fungi spawn in the spawn culture medium; A medium forming step of forming a hippocampal medium based on seahorse; An inoculation step of inoculating the cultured Cordyceps sinensis seed in the formed hippocampus medium; And culturing the inoculated Cordyceps spp. In the hippocampal medium to form a mycelium and a fruiting body.

In addition, the seahorse provides a cultivation method of seahorse cordyceps including cultured seahorse.

The seahorse also provides a cultivation method of hippocampus Cordyceps, including big-belly seahorse or pot-bellied seahorse.

In addition, the medium forming step provides a cultivation method of hippocampus Cordyceps sinensis which is a step of forming a hippocampus medium based on the hippocampus dried for 5 to 10 hours at 30 to 60 ℃ discharged the excreta.

In addition, the medium forming step further includes a desalting step of removing the salts of the hippocampus, wherein the desalting step is a mixture of brine having a salinity equivalent to that of the hippocampus and the dried hippocampus under alcohol. It provides a cultivation method of hippocampus Cordyceps comprising the step of.

In addition, the desalting step provides a cultivation method of hippocampus Cordyceps, further comprising the step of stationary treatment for 15 to 20 hours and the filtration treatment of the hippocampus after the mixing treatment step.

In addition, the culture step provides a cultivation method of hippocampus Cordyceps sinensis which is a step of inoculating the liquid seed or solid seedling of the Cordyceps sinensis in the aseptic culture medium.

In addition, the inoculation step is a sterilization of the hippocampus medium and then dissecting the outer skin of the hippocampus to provide a cultivation method of the hippocampus Cordyceps, which is a step of inoculating the cultured Cordyceps spp.

In addition, the growth step is incubated with mycelium at the conditions of temperature 23 to 27 ℃, humidity 65 to 75%, the step of growing after fruiting the fruiting body by irradiation with light at the temperature of 15 to 20 ℃, humidity 80 to 95% Provides a method of cultivating seahorse cordyceps.

In another aspect, the present invention provides a hippocampus Cordyceps sinensis comprising the nutrients of the seahorse (seahorse).

In addition, the nutrient is selected from the group consisting of taurine (taurine), arginine (arginine), aspartic acid (aspartic acid), alanine (alanine), glycine (glycine), proline (proline) and glutamic acid (glutamic acid) Provided is a hippocampus Cordyceps sinensis comprising one or more amino acids.

In addition, the nutrients include calcium (calcium), magnesium (magnesium), potassium (potassium), sodium (sodium), iron (iron), zinc (zinc), manganese (manganese), copper (copper) and chromium (chromium) It provides a hippocampus Cordyceps sinensis comprising any one or more minerals selected from the group consisting of.

In another aspect, the present invention provides a food comprising the hippocampus Cordyceps sinensis as one component.

By providing a hippocampus Cordyceps sinensis containing new nutrients that can not be expected in the general Cordyceps sinensis by the present invention, by providing a food containing it as one component to stimulate sex hormone secretion, and provides the effects of anti-aging and antithrombosis .

In addition, by using the hippocampus Cordyceps sinensis prepared by the present invention as a herbal medicine can be used for the treatment of diseases such as asthma, heart disease, fracture.

Prior to describing the present invention in detail below, it is understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention, which is limited only by the scope of the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art unless otherwise indicated.

Throughout this specification and claims, unless otherwise indicated, the termcomprise, constitutes, and configure means to include the referenced article, step, or group of articles, and step, and any other article It is not intended to exclude a stage or group of things or groups of stages.

On the other hand, various embodiments of the present invention can be combined with any other embodiment unless clearly indicated to the contrary. Any feature indicated as particularly preferred or advantageous may be combined with any other feature and features indicated as preferred or advantageous. Hereinafter, with reference to the accompanying drawings will be described an embodiment of the present invention and the effects thereof.

Provided is a method for cultivating Cordyceps sinensis based on the hippocampus according to an embodiment of the present invention.

Method of cultivating the hippocampus Cordyceps sinensis of the present invention is a culture step (S1) of cultivating the species of Cordyceps sinensis, a medium formation step (S2) to form a hippocampus medium based on the hippocampus, the inoculation step of inoculating Cordyceps fungi cultured in the hippocampus medium (S3), and the growth step (S4) of culturing the inoculated Cordyceps fungi spawn to form mycelium and fruiting body.

The culturing step (S1) according to an embodiment of the present invention is a step of inoculating a liquid spawn or a solid spawn of Cordyceps sinensis in a spawn culture medium in a sterile state, and as a Cordyceps spawn seed, cultured in a liquid culture solution using potato or soy flour. General solid spawn grown in a solid medium such as liquid spawn or brown rice can be used. As the spawn culture medium, any medium such as a liquid medium or a solid medium, a natural medium or a synthetic medium can be used, and can be cultured Cordyceps fungi using the hippocampal medium prepared in the medium forming step (S2) described later.

Cordyceps fungi can be applied without restriction, such as Paecilomyces japonica bacteria or Militaris cordyceps fungi (Cordyceps militaris).

More specifically, after the sterilization of the prepared culture medium is inoculated with the fungus. Sterilization is preferably subjected to secondary high temperature sterilization at 100 to 120 ° C. after the first low temperature sterilization at 80 to 100 ° C. After cooling the sterilized medium, the fungus is inoculated into the medium.

Medium formation step (S2) according to an embodiment of the present invention is a step of forming a culture medium based on the hippocampus, by stopping the feeding for at least 24 hours to discharge the excretion of the hippocampus to form a hippocampus medium do.

Cultured seahorses may be used as the hippocampus, and preferably, big-belly seahorses or pot-bellid seahorses are used. However, the present invention is not limited thereto, and any type of seahorse, such as a Great Seahorse (H. kelloggi), a Spotted seahorse (H. kuda), or a Longnose seahorse (H. trimaculatus), may be used without limitation. .

More specifically, the hippocampus to be used as the medium stops feeding at least 24 hours, preferably 24 to 48 hours, to excrete feces. It is preferable to dry the hippocampus from which excreta was discharged at 30-60 degreeC for 5 to 10 hours. However, depending on the condition of the hippocampus, the desired culture conditions, and the type of Cordyceps fungus, the drying temperature or drying time can be controlled. The dried hippocampus can be used as a medium by entering a glass bottle, a plastic bottle, a bag, or the like or as a medium itself.

Medium formation step (S2) according to an embodiment of the present invention may further include a desalination step (S201) for adjusting the salinity (salinity) of the hippocampus or a dehydration step (S202) for adjusting the water content of the hippocampus. In addition, it may include both the desalting step (S201) and the dehydration step (S202), in which case it is preferable to perform the desalting step (S201) first.

Through the desalting step (S201) of the hippocampus according to an embodiment of the present invention, the salinity of the hippocampus is adjusted to a level suitable for cultivating Cordyceps sinensis. In the desalting step (S201), a brine having a salinity equivalent to that of the hippocampus and a hippocampus are mixed under alcohol, and after stationary for 15 to 20 hours, the hippocampus is filtered to control salinity. Getting a hippocampus.

The brine used in the desalting step S201 includes seawater or saline and includes a solution containing salts such as calcium chloride. The salinity of brine is equivalent to that of the hippocampus used as a medium.

In addition, the desalting step (S201) is a step of precipitating salts by mixing under alcohol to lower the solubility of salts. It is preferable to use ethyl alcohol or pretanol A among alcohols.

In the desalting step (S201), 80 to 120 parts by mass of hippocampus is mixed with respect to 100 parts by mass of brine, and the stationary treatment is performed under 500 to 1000 parts by mass of alcohol. The salted seahorse is filtered off and washed to obtain a salted seahorse.

Through the dehydration step (S202) of the hippocampus according to an embodiment of the present invention, the moisture content of the hippocampus is adjusted to a level suitable for cultivating Cordyceps sinensis. It is preferable to dry the hippocampus or the salinity-controlled hippocampus by evaporation of moisture in the hippocampus under 20 to 40 ° C. If the water content is high, the fruiting body germinates well, but the mycelium is not well cultured. If the water content is low, the mycelium is well cultured but the fruiting body does not germinate. Therefore, there is a need for a process for controlling the water content of the hippocampus to an appropriate level. The drying temperature or drying time may be adjusted depending on the condition of the hippocampus, the desired culture conditions, and the type of Cordyceps inoculated. The hippocampus alone, ie, 100% hippocampus may be composed of a medium or a hippocampus mixed medium in which other media components are additionally mixed.

Inoculation step (S3) according to an embodiment of the present invention is a step of inoculating the cultured Cordyceps spp. Into the hippocampus medium that hosts the formed hippocampus, and disinfects the outer skin of the hippocampus after sterilizing the prepared hippocampus medium in the process. Then inoculate the Cordyceps fungi cultured in the hippocampus. After cooling the sterile hippocampal medium, inoculated aseptically, liquid spawn or solid spawn of Cordyceps sinensis below 20 ° C.

As a species of Cordyceps sinensis, a general liquid spawn cultured in a liquid medium using potato or soy flour, or a general solid spawn cultured in a solid medium such as brown rice can be used, and as described above, the seed spawn was cultured in a hippocampus medium according to the present invention. You can also use

Growth step (S4) according to an embodiment of the present invention is a step of culturing the inoculated Cordyceps fungi spawn to form a mycelium and fruiting body, it comprises a step of culturing with Cordyceps fungus mycelium in a culture room of the appropriate conditions. The culture conditions of Cordyceps mycelium are made at a specific culture temperature and a specific room humidity, so that the seeds are spread evenly in the medium to complete the culture of the mycelium. In general, when incubated for 8 to 10 days at a temperature of 23 to 27 ° C. and a humidity of 65 to 75%, the spawn is spread evenly in the medium to culture the mycelia.

In addition, the growth step (S4) according to an embodiment of the present invention includes a step of growing the Cordyceps mycelium cultured in the above process into Cordyceps fruiting body under appropriate conditions. Growth conditions of Cordyceps sinensis are made at a certain temperature and humidity. Typically, the fruiting body comes out when irradiated with light of 100 to 200lx at a temperature of 15 to 20 ℃, humidity 80 to 95%, the fruiting body grows when the light of 100 to 400lx on the fruiting body produced under the same conditions . In the case of Militaris Cordyceps sinensis, the fruiting body comes out when the light of about 800 to 1000lx for about 3 days, the fruiting body grows when 100 to 400lx light is irradiated on the fruiting body.

When the fruiting body grows more than 5cm, harvesting takes about 2 weeks from the time of planting to the harvesting time of snow cordyceps, and about 4 weeks in the case of Militaris cordyceps. Total planting days take about 30 days. When harvesting Cordyceps sinensis, the host seahorse and fruiting body are harvested together.

Hippocampus Cordyceps sinensis cultivated by the cultivation method according to an embodiment of the present invention includes a nutrient of seahorse (seahorse) used as a host. In addition, the hippocampus Cordyceps sinensis according to an embodiment of the present invention is cultivated based on a bigbelly seahorse or pot-bellid seahorse, and includes nutrients of a bigbelly seahorse or a potbelly seahorse. do. However, any type of seahorse, such as Great seahorse (H. kelloggi), Spotted seahorse / H. Kuda, or Longnose seahorse / H. Trimaculatus, may be included without limitation.

Major nutrients included include arginine, aspartic acid, alanine, glycine, proline and glutamic acid, including taurine, which has high medicinal value. Contains 20 amino acids, calcium, magnesium, potassium, sodium, sodium, iron, zinc, manganese, copper, chromium Abundant inorganic elements such as

In another aspect of the present invention, there is provided a food comprising a hippocampus Cordyceps sinensis according to one embodiment of the present invention as one component. In addition, Cordyceps sinensis of the present invention can be freeze-dried according to a conventional method for commercialization, or can be used as a water extract or liquor. Cordyceps sinensis containing nutrients of the hippocampus is used as a therapeutic agent for sexual dysfunction, renal dysfunction, and excessive cholesterol. It also acts on the liver and kidney to promote blood circulation, antithrombotic and anti-aging effect.

Example

Example 1

As a seed for cultivation of Cordyceps sinensis, a liquid culture of the seedlings of Cordyceps militaris was used. Cordyceps sinensis inoculum was used to culture the Cordyceps fungi spawn at 22-30 ℃ for 5 days using potato sugar medium (PD broth).

The hippocampus used the Big Valley seahorse farmed in Jeju Island. Feeding the hippocampus was stopped for 36 hours to discharge the excreta and dried for 6 hours at 40 ℃.

As a hippocampal medium, a medium having a composition of 100 g of dried seahorse, 30 g of water, and 2 g of sugar was formed. The medium was filled in a plastic container (about 850 ml), and sterilized for 120 minutes at a temperature of 121 ° C. and 1.5 atm.

After inoculating the inoculum prepared in advance by liquid culture into the hippocampus medium cooled by autoclaving, incubate in a culture chamber at a temperature of 25 ° C. for about 7-10 days, and then spreading the mycelia sufficiently in the medium. Then, the medium is removed from the plastic container and placed on a plate. After moving to a culture room with a temperature of 20 ° C., a humidity of 90%, and an illuminance of 500 Lux, it was incubated for about 3 days, and a constellation started to form.

Example 2

After feeding off the hippocampus for 36 hours, the excreta was discharged and dried at 30 ° C. for 5 hours. Then, 100 g of brine with 13% salinity and 100 g of dried seahorse were added to 800 g of pretanol A, mixed and left for 15 hours. The hippocampus was filtered off and evaporated at room temperature to give dried hippocampus.

Hippocampus Cordyceps was grown in the same manner as in Example 1 except that the desalted and dried hippocampus was used as the base.

Experimental Example

(1) Measurement of days of culture and days of growth

Cultivation of mushrooms by the method of the above example was harvested when the diameter of the fruiting body was 7cm, and the number of culture days and growth days until harvesting was measured and shown in Table 1 below. Here, the culture days are days required for mycelia to grow from the spawn inoculation to the bottom of the bottle, and the growth days are the days taken from the fruiting body to the harvest date.

	Incubation days	Growth
Example 1	40-42	10-15
Example 2	32-35	7-13

Referring to the results shown in Table 1, it can be seen that when the desalted hippocampus was used as a medium (Example 2), the culture days and growth days were shortened as compared with the case of using the dried hippocampus (Example 1). .

(2) general component analysis

General ingredients were performed by the Food Code (2008), crude ash was analyzed by dry calcification method, crude fat by ether extraction method, and crude protein by Kjeldahl method. For free sugar analysis, 1 ~ 5 g of sample was dissolved by adding 25 mL of water, extracted with acetonitrile, diluted with 0.45 μm membrane filter (Millipore, USA) according to Food Code (2008), and then purified by HPLC 2695 (Waters, USA).

Analysis of the general components of the hippocampus Cordyceps sinensis obtained by cultivating according to the method of the above embodiment is shown in Table 2 below.

ingredient	Example 1	Example 2	Unit
Protein	52.8	64.1	%
Saccharide	12.5	15.1	g / 100g
Invert sugar	4.5	5.5	g / 100g

<General ingredient analysis result>

As shown in Table 2, in the case of Cordyceps sinensis grown on the desalted, dried seahorse of Example 2, 64.1% protein, 15.1 g / 100g of saccharides, the case of Cordyceps sinensis grown on the dried hippocampus of Example 1 (Protein 52.8%, sugar 12.5 g / 100g).

(3) inorganic component analysis

In the mineral analysis, 1 g of the sample was taken and carbonized according to the Food Code (2008), and then heated at a temperature of 550 ° C. for several hours to incubate until white gray-white ash was obtained. This ash was sequentially decomposed using hydrochloric acid, diluted, filtered and then quantified by atomic absorption spectrometry using an ICP analyzer (ICP-OES, Varian).

Inorganic component analysis results of the hippocampus Cordyceps sinensis obtained by cultivation according to the method of the embodiment is shown in Table 3.

ingredient	Example 1	Example 2
Ca	39.48	32.9
Mg	84.1	96.9
K	181.8	196.95
Na	100.92	109.33
Fe	23.28	25.22
Zn	7.56	8.19
Mn	1.82	1.68
Cu	1.8	1.8
Cr	1.4	1.5

As a result of analyzing the inorganic component content of seahorse Cordyceps sinensis, Ca, Mg, K, Na, Fe, Zn, Mn, Cu, Cr were detected. The hippocampus Cordyceps sinensis obtained according to the method of Example 2 was found to have a somewhat higher content of Mg, K, Na, Fe, Zn, Cr, etc. than the hippocampus Cordyceps sinus obtained according to the method of Example 1.

(4) amino acid analysis

Amino acid analysis was performed by AccQTag amino acid analysis method (Waters). Analysis conditions were HPLC (Waters 2695, USA) and the column was detected by a fluorescence detector (EX: 250 nm, EM: 395 nm) using AccQTag (3.9 × 150 mm) and the analysis conditions are shown in Table 4 below. The results are shown in Table 5 below.

Instrument	HPLC (waters 2475) Waters 2475 Multi λ Fluorescence Detector
Detector	Grain: 1 Filter: 327nm Excitation: 297nmEmission: 395nm
Pump	Waters 2695 separations ModuleAccQTag column for hydrolysate amino acid analysis
Column	3.9 Aqneous buffer, Water AccQTag Eluent A
Mobile phase	Acetonitrilewater
Flow rate	1.0 ml / min
Injection volume	10 μl

ingredient	Example 1	Example 2
arginine	0.945	1.044
aspartic acid	1.222	1.664
alanine	0.851	1.090
glycine	0.522	0.596
taurine	0.543	0.692
proline	0.604	0.669
glutamic acid	1.575	1.606
serine	6.262	7.360
histidine	0.274	0.357
threonine	0.386	0.377
methionine	0.134	0.086
isoleucine	0.228	0.131
Total	9.710	10.566

As shown in Table 5, the amino acid content was detected and identified 12 amino acids, including taurine (taurine). The total amino acid content of the hippocampus Cordyceps sinensis according to the method of Example was 9.710g and 10.566g per 100g, respectively, and taurine contained 0.712g / 100g and 0.878g / 100g.

Features, structures, effects, and the like illustrated in the above-described embodiments may be combined or modified with respect to other embodiments by those skilled in the art to which the embodiments belong. Therefore, contents related to such combinations and modifications should be construed as being included in the scope of the present invention.

Claims (13)

1. Culturing the Cordyceps spp. In the spawn culture medium;

   A medium forming step of forming a hippocampal medium based on seahorse;

   An inoculation step of inoculating the cultured Cordyceps sinensis seed in the formed hippocampus medium; And

   Cultivation method of the hippocampus Cordyceps comprising the; growing step of culturing the inoculated Cordyceps fungi spawn in the hippocampal medium to form a mycelium and fruiting body.
2. The method of claim 1,

   The seahorse is a cultivation method of seahorse Cordyceps sinensis comprising cultured seahorse.
3. The method of claim 1,

   The seahorse is a big-belly seahorse or pot-bellied seahorse (pot-bellied seahorse) comprising a seahorse cordyceps cultivation method.
4. The method of claim 1,

   The medium forming step is a cultivation method of the hippocampus Cordyceps sinensis which is a step of forming a hippocampus medium based on the hippocampus dried for 5 to 10 hours at 30 to 60 ℃ to discharge the excrement.
5. The method of claim 4, wherein

   The medium forming step further includes a desalting step of removing the salts of the hippocampus,

   The desalting step comprises the step of mixing the brine (brine) having the salinity equivalent to the hippocampus and the dried hippocampus under alcohol (mixing) under the alcohol (cultivation method).
6. The method of claim 5,

   The desalting step is after the mixing treatment,

   Stationary treatment for 15 to 20 hours, and

   A cultivation method of the hippocampus Cordyceps sinensis further comprising the step of filtration treatment for the hippocampus.
7. The method of claim 1,

   The culturing step is a method of culturing seahorse Cordyceps sinensis which is a step of inoculating the liquid seedling or solid seedling of the Cordyceps sinensis in the aseptic culture medium in aseptic state.
8. The method of claim 1,

   The inoculation step is a method for cultivating the hippocampus Cordyceps sinensis in which the cultured Cordyceps spp.
9. The method of claim 1,

   The growth step is a step of culturing the mycelium under the conditions of the temperature of 23 to 27 ℃, humidity 65 to 75%, and after growing fruiting bodies by irradiating light at the temperature of 15 to 20 ℃, humidity of 80 to 95% Cultivation method of seahorse cordyceps.
10. Hippocampus Cordyceps sinensis containing nutrients from seahorses.
11. Contains nutrients from seahorses,

    The nutritional component of any one selected from the group consisting of taurine, arginine, arginine, aspartic acid, aspartic acid, alanine, glycine, glycine, proline and glutamic acid Hippocampus Cordyceps sinensis containing more than one amino acid.
12. Contains nutrients from seahorses,

    The nutritional component consists of calcium (calcium), magnesium (magnesium), potassium (potassium), sodium (sodium), iron (iron), zinc (zinc), manganese (manganese), copper (copper) and chromium (chromium) Hippocampus Cordyceps sinensis comprising any one or more minerals selected from the group to be described.
13. Food comprising the seahorse cordyceps sinensis of claim 10 as one component.