WO2016182158A1 - Composition contenant de l'extrait de lactuca scariola comme principe actif pour la prévention ou le traitement de la maladie parodontale - Google Patents

Composition contenant de l'extrait de lactuca scariola comme principe actif pour la prévention ou le traitement de la maladie parodontale Download PDF

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Publication number
WO2016182158A1
WO2016182158A1 PCT/KR2015/013051 KR2015013051W WO2016182158A1 WO 2016182158 A1 WO2016182158 A1 WO 2016182158A1 KR 2015013051 W KR2015013051 W KR 2015013051W WO 2016182158 A1 WO2016182158 A1 WO 2016182158A1
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WO
WIPO (PCT)
Prior art keywords
extract
active ingredient
periodontal disease
lettuce
lactuca scariola
Prior art date
Application number
PCT/KR2015/013051
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English (en)
Korean (ko)
Inventor
김은실
오경희
백광현
김양선
Original Assignee
대한민국(환경부 국립생물자원관장)
영남대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 대한민국(환경부 국립생물자원관장), 영남대학교산학협력단 filed Critical 대한민국(환경부 국립생물자원관장)
Publication of WO2016182158A1 publication Critical patent/WO2016182158A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea

Definitions

  • the present invention relates to a composition for the prevention or treatment of periodontal disease, containing the thorn lettuce extract as an active ingredient, more specifically, the antibacterial composition against periodontitis bacteria containing thorn lettuce extract as an active ingredient, thorn lettuce extract as an active ingredient It relates to a pharmaceutical composition for the prevention or treatment of periodontal disease, comprising and a health functional food composition for the prevention or improvement of periodontal disease, containing the spinach lettuce extract as an active ingredient.
  • Periodontitis is an inflammation of the supporting tissues surrounding the teeth, which is a chronic inflammatory disease caused by bacteria and toxins in the microbial plaque. Periodontitis is an idiom and is often referred to as flavor, and it is also called periodontal disease or periodontal disease. When periodontitis occurs, the gums settle down and the roots of the teeth appear, causing the symptom.
  • Various oral diseases including dental caries, gingivitis and periodontitis, can be caused by a variety of causes.
  • the causes can be broadly divided into systemic causes and local causes, more important of which are known to be local causes.
  • the most important local cause is bacteria that reside in the oral cavity, ie pathogenic microorganisms.
  • the pathogenic microorganisms resident in the oral cavity attach to the tooth surface, and after a few hours, they form a bacterial community called plaque and multiply, and initially the pathogenic microorganisms adhere to the surface of the upper teeth.
  • plaque By forming plaque, but gradually progressing, plaque is also formed on the tooth surface under the gingiva.
  • the pathogenic microorganisms produce an acid by using sugars in the oral cavity, and the acid can cause tooth decay by demineralizing the mineral that is the main component of the tooth.
  • Microorganisms that play the most important role in inducing oral diseases as described above include Streptococcus mutans , which causes tooth decay, and Porphyromonas gingivalis , which causes periodontitis, and the like.
  • antimicrobial agents including antibiotics having bactericidal and bacteriostatic effects on the microorganisms have been developed as inhibitors and treatments for tooth decay, periodontal disease or gingivitis.
  • Conventionally used materials include compounds containing fluorine and chlorine, such as sodium copper chlorophyllin, sodium fluoride or benzethonium chloride, benzoic aicd, and the like.
  • Korean Patent No. 0835899 discloses a composition for preventing or treating periodontal disease comprising a ginger extract
  • Korean Patent No. 0991857 discloses a composition for preventing or treating periodontal disease comprising a nut extract. It is different from the composition for preventing or treating periodontal disease, which contains the thorn lettuce extract of the present invention as an active ingredient.
  • the present invention is derived from the above requirements, the present inventors have confirmed that the present invention by showing that the Lactuca scariola extract has antimicrobial activity against Porphyromonas gingivalis , which is a periodontitis bacterium, Completed.
  • the present invention provides a composition for antibacterial against periodontitis bacteria containing Lactuca scariola as an active ingredient.
  • the present invention provides a pharmaceutical composition for the prevention or treatment of periodontal disease, containing the extract of the lettuce ( Lactuca scariola ) as an active ingredient.
  • the present invention provides a dietary supplement for the prevention or improvement of periodontal disease containing spinach lettuce ( Lactuca scariola ) extract as an active ingredient.
  • composition containing the extract of the lettuce ( Lactuca scariola ) according to the present invention as an active ingredient shows excellent antimicrobial activity against the periodontal bacterium Porphyromonas gingivalis , a pharmaceutical composition for preventing or treating periodontal disease And it can be used as a dietary supplement for preventing or improving periodontal disease.
  • the present invention provides a composition for antimicrobial against periodontitis bacteria containing Lactuca scariola extract as an active ingredient.
  • the periodontal bacterium is Porphyromonas gingivalis , Tannerella forsythia , Fusobacterium nucleatum and Actinobacilli actinomy Actinobacillus actinomycetemcomitans and the like, preferably may be, but not limited to Porphyromonas gingivalis ( Porphyromonas gingivalis ).
  • the thorn lettuce extract may be an extract using a leaf or stem portion of the ground of the plant, but is not limited thereto.
  • the thorn lettuce extract may be water, methanol, ethanol, propanol, butanol or a mixed solvent extract of dried thorn lettuce, preferably a methanol extract of dried thorn lettuce Specifically, after drying the visible lettuce cut to 2-3 cm using soybeans for 2-4 days at a temperature of 30-50 °C, 75-85% (v / v) of the dry weight of 400-600 g Methanol may be prepared by extracting and filtering by using an ultrasonic device.
  • the present invention provides a pharmaceutical composition for the prevention or treatment of periodontal disease containing the spiny lettuce extract as an active ingredient.
  • Periodontitis gingivitis and periodontitis depending on the severity of the disease. It is a relatively light and fast-recovering form of periodontal disease that is limited to the gums, ie soft tissues, is called gingivitis, and when this inflammation progresses to the gums and around the gum bone, it is called periodontitis.
  • Extracts of the present invention may comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.
  • the pharmaceutical dosage forms of the extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.
  • the extracts according to the present invention may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories, and sterile injectable solutions, respectively, according to conventional methods. have.
  • Carriers, excipients and diluents that may be included in the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl And various compounds or mixtures including cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil and the like.
  • Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed.
  • lubricants such as magnesium stearate and talc are also used.
  • Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin.
  • Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories.
  • the non-aqueous solvent and suspending agent propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used.
  • As the base of the suppository witepsol, macrogol, tween 60, cacao butter, laurin butter, glycerogelatin and the like can be used.
  • Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art.
  • the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 100 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.
  • the extract of the present invention can be administered to mammals such as mice, mice, livestock, humans, etc. by various routes. All modes of administration can be expected, for example oral, by injection.
  • the present invention provides a health functional food composition for preventing or improving periodontal disease containing the thorn lettuce extract as an active ingredient.
  • the thorn lettuce extract of the present invention shows excellent antimicrobial activity against the periodontitis Porphyromonas gingivalis bacteria can be usefully used for the prevention or improvement of periodontal disease such as periodontitis.
  • the extract of the present invention When the extract of the present invention is used as a food additive, the extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method.
  • the mixed amount of the active ingredient can be suitably determined according to the purpose of use (prevention, health or therapeutic treatment).
  • the extract of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on the raw material in the manufacture of food or beverage.
  • the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .
  • the kind of food there is no particular limitation on the kind of food.
  • the food to which the extract can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, dairy products including other noodles, gums, ice cream, various soups, drinks, tea, drink, Alcoholic beverages and vitamin complexes, and includes all of the dietary supplements in the conventional sense.
  • Extract of Lactuca scariola L. was used on the ground part of the spiny lettuce, and leaf and stem parts were used.
  • the leaves and stems of the spiny lettuce identified by the plant taxonomy were cut evenly to about 2.54 cm using small beans, and then dried at 40 ° C. in a drying oven for 3 days.
  • extraction was performed using an ultrasonic instrument in 80% methanol, and these liquids were filtered through a membrane filter (0.2 ⁇ m) and then the solvent was removed in vacuo to leave a methanol extract.
  • the thorn lettuce extract is a methanol extract mainly containing a trace component of the thorn lettuce. All solvents used were analytical grade, purchased from Sigma-Aldrich (USA) or Merck (USA).
  • the antimicrobial activity of thorn lettuce extract against periodontitis-causing bacteria was assayed. Briefly, 1 ⁇ 10 7 cfu / ml of Porphyromonas gingivalis W83 (ATCC BAA-1703) was prepared in a 5 ml serum bottle, respectively, and the lettuce lettuce methanol extracts were 3.6, 1.8 and Each was treated at a concentration of 0.6 mg / ml. Then, after maintaining the anaerobic state for 24 hours at 37 °C, the growth inhibition rate of the periodontal causative bacteria was analyzed. A total of three replicate experiments were performed, and each experiment was repeated three times.
  • the antimicrobial activity of methanol extract of spiny lettuce against periodontitis is shown in Table 1.
  • the visible lettuce extract showed a high growth inhibition rate of about 90% at 0.6 mg / ml concentration, 94% at 1.8 mg / ml concentration and 100% growth inhibition at 3.6 mg / ml concentration.
  • the thorn methanol extract can be used for the prevention or treatment of periodontal disease due to the high antibacterial activity against periodontitis.

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne : une composition antimicrobienne contenant de l'extrait de Lactuca scariola comme principe actif contre les bactéries de la parodontite ; une composition pharmaceutique contenant de l'extrait de Lactuca scariola comme principe actif pour la prévention ou le traitement de la maladie parodontale ; et une composition alimentaire fonctionnelle contenant de l'extrait de Lactuca scariola comme principe actif pour la prévention ou l'amélioration de la maladie parodontale.
PCT/KR2015/013051 2015-05-12 2015-12-02 Composition contenant de l'extrait de lactuca scariola comme principe actif pour la prévention ou le traitement de la maladie parodontale WO2016182158A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020150066184A KR101574678B1 (ko) 2015-05-12 2015-05-12 가시상추 추출물을 유효성분으로 함유하는 치주질환 예방 또는 치료용 조성물
KR10-2015-0066184 2015-05-12

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WO2016182158A1 true WO2016182158A1 (fr) 2016-11-17

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WO (1) WO2016182158A1 (fr)

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KR102373594B1 (ko) * 2020-01-22 2022-03-14 제너럴바이오(주) 감국 추출물을 유효성분으로 포함하는 구강질환의 예방, 개선 또는 치료용 조성물

Citations (4)

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KR20120011351A (ko) * 2010-07-22 2012-02-08 노창현 매실을 함유하는 구강구취용 치약 조성물
KR101121755B1 (ko) * 2009-05-15 2012-03-23 황철수 치아미백용 치약 조성물 및 그 제조방법
KR20120049488A (ko) * 2010-11-09 2012-05-17 전라남도 야콘 추출물을 유효성분으로 함유하는 충치와 치주질환 예방 또는 치료용 약학조성물 및 식품 조성물
KR101211937B1 (ko) * 2012-09-18 2012-12-13 주식회사 들레네 101가지 산야초 추출액을 이용한 항염 효과를 갖는 기능성 산야초 발효물

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KR20120011351A (ko) * 2010-07-22 2012-02-08 노창현 매실을 함유하는 구강구취용 치약 조성물
KR20120049488A (ko) * 2010-11-09 2012-05-17 전라남도 야콘 추출물을 유효성분으로 함유하는 충치와 치주질환 예방 또는 치료용 약학조성물 및 식품 조성물
KR101211937B1 (ko) * 2012-09-18 2012-12-13 주식회사 들레네 101가지 산야초 추출액을 이용한 항염 효과를 갖는 기능성 산야초 발효물

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MOON, SEONG I1 ET AL.: "Isolation and Characterization of Bio-active Materials from Prickly Lettuce (Lactuca serriola", JOURNAL OF LIFE SCIENCE, vol. 19, no. 2, 2009, pages 206 - 212, XP055328033 *

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