Classifications

• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/38—Products with no well-defined composition, e.g. natural products
  • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
  • C11D3/38663—Stabilised liquid enzyme compositions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
  • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
  • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B08—CLEANING
  • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
  • B08B1/00—Cleaning by methods involving the use of tools
  • B08B1/10—Cleaning by methods involving the use of tools characterised by the type of cleaning tool
  • B08B1/14—Wipes; Absorbent members, e.g. swabs or sponges
  • B08B1/143—Wipes
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
  • C11D1/66—Non-ionic compounds
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
  • C11D17/04—Detergent materials or soaps characterised by their shape or physical properties combined with or containing other objects
  • C11D17/049—Cleaning or scouring pads; Wipes
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/20—Organic compounds containing oxygen
  • C11D3/2075—Carboxylic acids-salts thereof
  • C11D3/2079—Monocarboxylic acids-salts thereof
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/34—Organic compounds containing sulfur
  • C11D3/3481—Organic compounds containing sulfur containing sulfur in a heterocyclic ring, e.g. sultones or sulfolanes
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/38—Products with no well-defined composition, e.g. natural products
  • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
  • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
  • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
  • A61L2202/20—Targets to be treated
  • A61L2202/24—Medical instruments, e.g. endoscopes, catheters, sharps
• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
  • C11D2111/10—Objects to be cleaned
  • C11D2111/14—Hard surfaces
  • C11D2111/20—Industrial or commercial equipment, e.g. reactors, tubes or engines

Definitions

• the present disclosure relates to the field of rinsing and disinfection, and particularly relates to an enzyme solution, an enzyme-containing non-woven fabric, and methods of preparation and use thereof.
• Rinsing and disinfection represents a key step in the treatment of medical devices after use.
• the step of pre-rinsing or pre-cleaning can significantly increase the success rate of subsequent rinsing and disinfection, prolong the service life of the medical devices, and reduce the risk of contamination.
• doctors will, after removing an endoscope from the body of a patient, immediately use a wet tissue or a wet gauze containing rinsing solution to wipe off contaminants on the outer surface of the endoscope to complete the pre-cleaning, prior to detaching the endoscope from a light source or a video processor.
• the present disclosure provides an enzyme solution comprising 0.1 to 10 wt.% of a nonionic surfactant; 0.01 to 1.2 wt.% of an enzyme; 0.03 to 15 wt.% of a normal-temperature enzyme stabilizer; 0.75 to 6 wt.% of an isothiazolinone; 0.1 to 1 wt.% of a benzoisothiazolinone; 0.1 to 1 wt.% of sodium benzoate; 6.9 to 10 wt.% of a solubilizer; 0.15 to 0.5 wt.% of a pH adjuster,; and 60 to 85 wt.% of water, based on the total weight of the enzyme solution; the enzyme solution having a pH value of 6 to 10.
• the enzyme solution comprises 0.1 to 0.7 wt.% of sodium benzoate. In some aspects of the present disclosure, the enzyme solution comprises 0.1 to 0.5 wt.% of sodium benzoate. In some aspects of the present disclosure, the enzyme solution comprises about 5 to 13 % normal-temperature enzyme stabilizer. In some aspects, the present disclosure the enzyme solution comprises about 10 to 11 % normal-temperature enzyme stabilizer.
• Nonionic surfactant is 0.1 to 0.7 wt.% of sodium benzoate. In some aspects of the present disclosure, the enzyme solution comprises 0.1 to 0.5 wt.% of sodium benzoate. In some aspects of the present disclosure, the enzyme solution comprises about 5 to 13 % normal-temperature enzyme stabilizer. In some aspects, the present disclosure the enzyme solution comprises about 10 to 11 % normal-temperature enzyme stabilizer.
• the nonionic surfactant helps to decrease the surface tension of the enzyme solution and increase the wetting and cleaning capability of the enzyme solution.
• the nonionic surfactant comprises at least one component selected from the group consisting of (i) a polymer based on at least one of ethylene oxide, propylene oxide and butylene oxide, (ii) an alkyl glucoside, (iii) a fatty alcohol polyoxyethylene ether, and (iv) an ammonium oxide.
• the polymer based on the at least one of ethylene oxide, propylene oxide and butylene oxide comprises at least one component selected from the group consisting of ethylene oxide / butylene oxide copolymer, and ethylene oxide / propylene oxide / butylene oxide copolymer.
• EH-6 commercially available from The Dow Chemical Company can be selected as the polymer based on the at least one of ethylene oxide, propylene oxide and butylene oxide.
• the alkyl glucoside has a molecular weight of 320 to 350.
• the alkyl glucoside comprises at least one component selected from the group consisting of decyl glucoside, octyl glucoside and dodecyl glucoside.
• Glucopon 425N/HH commercially available from BASF Corporation can be selected as the alkyl glucoside.
• the fatty alcohol polyoxyethylene ether comprises at least one component selected from the group consisting of C7 to C9 fatty alcohol polyoxyethylene ether, C12 to C16 fatty alcohol polyoxyethylene ether, and C9 to C11 fatty alcohol polyoxyethylene ether.
• A1058 commercially available from Harcros Corporation can be selected as the fatty alcohol polyoxyethylene ether.
• the nonionic surfactant is in an amount of 0.1 to 10 wt.%, based on the total weight of the enzyme solution.
• the cleaning capability of the enzyme solution may decrease; and when the nonionic surfactant is in an amount of more than 10 wt.%, the enzyme solution may suffer from excessive foaming, resulting in decreased cleaning capability of the enzyme solution.
• the enzyme helps to decompose the proteins, saccharides, fats, and celluloses in the contaminants associated with medical devices.
• the enzyme comprises at least one component selected from the group consisting of a proteinase, an amylase, a lipase, and a cellulase.
• Alcalase, Esperase, Everlase, Savinase16XL, Liquanase, or Polarzyme commercially available from Novozymes Corporation can be selected as the proteinase.
• Properase commercially available from Genencor Corporation can also be selected as the proteinase.
• Stainzyme, Termamyl 300L, BAN 480L, or Duramyl commercially available from Novozymes Corporation can be selected as the amylase.
• Lipolase commercially available from Novozymes Corporation or Purafect commercially available from Genencor Corporation can be selected as the lipase.
• Carezyme 4500 or Endolase commercially available from Novozymes Corporation can be selected as the cellulase.
• the enzyme is in an amount of 0.01 to 1.2 wt.% based on the total weight of the enzyme solution.
• the enzyme in the enzyme solution comprises 0.05 to 0.6 wt.% of a proteinase, 0.01 to 0.2 wt.% of an amylase, 0.01 to 0.2 wt.% of a lipase, and 0.01 to 0.2 wt.% a cellulase, based on the total weight of the enzyme solution.
• the enzyme is in an amount of less than 0.01 wt.%, the cleaning capability of the enzyme solution may decrease; and when the enzyme is in an amount of more than 1.2 wt.%, the stability of the enzyme solution may decrease.
• the normal-temperature enzyme stabilizer helps to maintain the activity of the enzyme under normal temperature (10 to 25oC) conditions.
• the normal-temperature enzyme stabilizer comprises at least one component selected from the group consisting of glycerol, propylene glycol, calcium chloride, magnesium chloride, and boric acid.
• the glycerol is commercially available from The Dow Chemical Company.
• the propylene glycol, calcium chloride, magnesium chloride, and boric acid are all commercially available from Sinopharm Chemical Reagent Co., Ltd., China.
• the normal-temperature enzyme stabilizer is present in an amount of 0.03 to 15 wt.%, based on the total weight of the enzyme solution.
• the normal-temperature enzyme stabilizer in the enzyme solution comprises 5 to 10 wt.% of glycerol and/or propylene glycol, and 0.03 to 0.7 wt.% of calcium chloride and/or magnesium chloride, based on the total weight of the enzyme solution.
• the normal-temperature enzyme stabilizer is in an amount of less than 0.03 wt.%, the stability of the enzyme in the enzyme solution under normal temperature (10 to 25oC) condition may decrease; and when the normal-temperature enzyme stabilizer is in an amount of more than 15 wt.%, the cleaning capability of the enzyme solution may decrease.
• an isothiazolinone, a benzoisothiazolinone, and sodium benzoate are used in combination.
• Inclusion of an isothiazolinone, a benzoisothiazolinone, and sodium benzoate together in the enzyme solution not only helps to maintain good stability of the enzyme in the enzyme solution under relatively high temperature (higher than 25oC) condition, but also contributes to the good mold-resistance and bacteria-resistance properties of the enzyme solution, as well as the good compatibility of the enzyme solution with non-woven fabrics.
• the isothiazolinone comprises at least one component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one (CMI) and 2-methyl-4-isothiazolin-3-one (MI).
• CMI 5-chloro-2-methyl-4-isothiazolin-3-one
• MI 2-methyl-4-isothiazolin-3-one
• Nipagurad CG commercially available from Clariant Corporation can be selected as the isothiazolinone.
• the benzoisothiazolinone comprises
• BIT Prexel GXL
• Lonza Corporation can be selected as the benzoisothiazolinone.
• the sodium benzoate has the molecular formula of C 6 H 5 CO 2 Na.
• the sodium benzoate is commercially available from Shanghai Shantou Guanghua Chemical Reagents Co., Ltd..
• the enzyme solution comprises 0.75 to 6 wt.% of an isothiazolinone, 0.1 to 1 wt.% of a benzoisothiazolinone, and 0.1 to 1 wt.% of sodium benzoate, based on the total weight of the enzyme solution.
• the stability of the enzyme in the enzyme solution under relatively high temperature (higher than 25oC) condition may decrease, the mold-resistance or bacteria-resistance properties of the enzyme solution may decrease, and the compatibility of the enzyme solution with the non-woven fabric may also decrease.
• the enzyme in the enzyme solution has particularly good stability under relatively high temperature (higher than 25oC) conditions, and the enzyme solution has particularly good mold-resistance and bacteria-resistance properties.
• the solubilizer helps to promote the dissolution of the ingredients in the enzyme solution.
• solubilizers are suitable for use in the enzyme solution of the present disclosure.
• the solubilizer comprises at least one component selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, xylene sulfuric acid, sodium xylene sulfate, and alkyl amine oxide.
• SXS 93 commercially available from Kuantum Corporation can be selected as the sodium xylene sulfonate.
• the solubilizer is present in an amount of 6.9 to 10 wt.%, based on the total weight of the enzyme solution. When the solubilizer is present in an amount less than 6.9 wt.%, some ingredients in the enzyme solution may not dissolve sufficiently; and when the solubilizer is in an amount of more than 10 wt.%, the activity of the enzyme in the enzyme solution may decrease. pH adjuster
• the pH adjuster is used for adjusting the pH value of the enzyme solution.
• pH adjusters may be suitable for use in the enzyme solution of the present disclosure.
• the pH adjuster comprises citric acid.
• the citric acid is commercially available from
• the pH adjuster is present in an amount of 0.15 to 0.5 wt.%, based on the total weight of the enzyme solution.
• the pH adjuster is present in an amount that is outside the above range, the enzyme solution may become too acidic (pH value being smaller than 6) or too alkaline (pH value being greater than 10), resulting in decreased activity of the enzyme in the enzyme solution.
• the enzyme solution has a pH in the range of 6 to 10. According to certain embodiments, the enzyme solution has a pH in the range of 7 to 9. Water
• water is used as the solvent. According to certain embodiments water is present in an amount of at least 60 wt.%, based on the total weight of the enzyme solution. According to certain embodiments water is present in an amount not greater than 85%, based on the total weight of the enzyme solution. When water is present in an amount less than 60 wt.%, some ingredients in the enzyme solution may not dissolve sufficiently, resulting in unwanted precipitation of some ingredients. When water is present in an amount of more than 85 wt.%, the ingredients in the enzyme solution may be overly diluted, thus decreasing the cleaning capability of the enzyme solution.
• the enzyme solution according to the present disclosure may also comprise 0.2 to 0.5 wt.% of a chelator, based on the total weight of the enzyme solution.
• the chelator may chelate metal ions which may be present as impurities in the enzyme solution, thus increasing the cleaning capability of the enzyme solution.
• a number of chelating agents may be used as the chelator in the enzyme solution disclosed herein.
• the chelator comprises at least one component selected from the group consisting of ethylendiamine tetraacetic acid (EDTA) and
• the EDTA commercially available from Sinopharm Chemical Reagent Co., Ltd., China can be selected as the ethylendiamine tetraacetic acid.
• Dissolvine D50 commercially available from
• AKZO-Nobel Corporation can be selected as the diethylenetriamine pentaacetic acid.
• the enzyme solution according to the present disclosure may also comprise 0.5 to 10 wt.% of a corrosion inhibitor, based on the total weight of the enzyme solution.
• the corrosion inhibitor helps to delay or prevent corrosion of the metal or rubber in contact with the enzyme solution.
• the corrosion inhibitor comprises at least one component selected from the group consisting of a borate ester, a polyether, and a higher alcohol.
• Crodacor BE-LQ-(AP) commercially available from Croda Corporation can be selected as the borate ester.
• the enzyme solution according to the present disclosure may also comprise 0.1 to 0.3 wt.% of a defoamer, based on the total weight of the enzyme solution.
• the defoamer helps to decrease or inhibit foaming of the enzyme solution.
• the defoamer comprises at least one component selected from the group consisting of a silicone oil, a polyether, and a higher alcohol.
• DK1247 commercially available from the Dow Corning Corporation can be selected as the defoamer.
• the present disclosure provides a method of preparing an enzyme solution, comprising the step of mixing the ingredients of the enzyme solution according to the present disclosure.
• the method of preparing the enzyme solution comprises the steps of sequentially adding 6.9 to 10 wt.% of a solubilizer, 0.1 to 10 wt.% of a nonionic surfactant, 0.75 to 6 wt.% of an isothiazolinone, 0.1 to 1 wt.% of a benzoisothiazolinone, 0.1 to 1 wt.% of sodium benzoate, 0.03 to 15 wt.% of a normal-temperature enzyme stabilizer into 30 to 60 wt.% of water to obtain a first solution; adding 0.15 to 0.5 wt.% of a pH adjuster to the first solution to obtain a second solution having a pH value of 6 to 10; and adding 0.01 to 1.2 wt.% of an enzyme and 3 to 51 wt.% of water into the second solution to obtain the enzyme solution.
• the method of preparing the enzyme solution comprises the steps of sequentially adding 6.9 to 10 wt.% of a solubilizer, 0.1 to 10 wt.% of a nonionic surfactant, 0.75 to 6 wt.% of an isothiazolinone, 0.1 to 1 wt.% of a benzoisothiazolinone, 0.1 to 1 wt.% of sodium benzoate, 0.03 to 15 wt.% of a normal-temperature enzyme stabilizer, 0.2 to 0.5 wt.% of a chelator, 0.5 to 10 wt.% of a corrosion inhibitor, and 0.1 to 0.3 wt.% of a defoamer into 30 to 60 wt.% of water to obtain a first solution; adding 0.15 to 0.5 wt.% of a pH adjuster into the first solution to obtain a second solution having a pH value of 6 to 10; and adding 0.01 to 1.2 wt.% of an enzyme and
• the present disclosure provides an enzyme-containing non-woven fabric, comprising a non-woven fabric and the enzyme solution according to the present disclosure dispersed in the non-woven fabric, wherein the non-woven fabric comprises 20 to 50 wt.% of viscose fiber and 50 to 80 wt.% of terylene fiber based on the total weight of the non-woven fabric; and the enzyme solution and the non-woven fabric are in a weight ratio of 1.5:1 to 7:1.
• the enzyme solution refer to the“Enzyme solution” section of the present specification.
• the viscose fiber can be prepared by the following method: alkalinizing natural cellulose to obtain alkalinized cellulose, reacting the alkalinized cellulose with carbon disulfide to obtain cellulose xanthate, dissolving the cellulose xanthate in a diluted alkaline solution to obtain a viscous solution (viscose), and fabricating the viscous solution (viscose) into the viscose fiber by a known wet spinning and post-treatment process.
• the terylene fiber can be prepared by the following method: subjecting pure terephthalic acid (PTA) or dimethyl terephthalate (DMT) and ethylene glycol (EG) to esterification or transesterification, followed by polycondensation reaction to obtain polyethylene terephtalate (PET), and fabricating the polyethylene terephtalate into terylene fiber by a known spinning and post-treatment process.
• PTA pure terephthalic acid
• DMT dimethyl terephthalate
• EG ethylene glycol
• PET polyethylene terephtalate
• the enzyme in the enzyme solution may be adsorbed by the non-woven fabric, reducing the compatibility of the enzyme solution with the non-woven fabric.
• the present disclosure provides a method of preparing the present disclosure
• enzyme-containing non-woven fabric comprising the step of allowing the enzyme solution according to the present disclosure to disperse into a non-woven fabric, the enzyme solution and the non-woven fabric being in a weight ratio of 1.5:1 to 7:1.
• the present disclosure provides use of the enzyme-containing non-woven fabric according to the present disclosure in cleaning medical devices.
• the enzyme-containing non-woven fabric according to the present disclosure can be used to contact medical devices for the purpose of cleaning the medical devices.
• the following embodiments are intended to describe the present disclosure in an illustrative rather than limitative manner.
• Embodiment 1 is an enzyme solution comprising 0.1 to 10 wt.% of a nonionic surfactant; 0.01 to 1.2 wt.% of an enzyme; 0.03 to 15 wt.% of a normal-temperature enzyme stabilizer; 0.75 to 6 wt.% of an isothiazolinone; 0.1 to 1 wt.% of a benzoisothiazolinone; 0.1 to 1 wt.% of sodium benzoate; 6.9 to 10 wt.% of a solubilizer; 0.15 to 0.5 wt.% of a pH adjuster; and 60 to 85 wt.% of water, based on the total weight of the enzyme solution; the enzyme solution having a pH value of 6 to 10.
• Embodiment 2 is an enzyme solution according to embodiment 1, wherein the nonionic surfactant comprises at least one component selected from the group consisting of (i) a polymer based on at least one of ethylene oxide, propylene oxide and butylene oxide, (ii) an alkyl glucoside, (iii) a fatty alcohol polyoxyethylene ether, and (iv) an ammonium oxide.
• the nonionic surfactant comprises at least one component selected from the group consisting of (i) a polymer based on at least one of ethylene oxide, propylene oxide and butylene oxide, (ii) an alkyl glucoside, (iii) a fatty alcohol polyoxyethylene ether, and (iv) an ammonium oxide.
• Embodiment 3 is an enzyme solution according to embodiment 1 or 2, wherein the enzyme comprises at least one component selected from the group consisting of a proteinase, an amylase, a lipase and a cellulase.
• Embodiment 4 is an enzyme solution according to embodiment 3, wherein the proteinase is in an amount of 0.05 to 0.3 wt.%, the amylase is in an amount of 0.01 to 0.1 wt.%, and the cellulase is in an amount of 0.01 to 0.1 wt.%, based on the total weight of the enzyme solution.
• Embodiment 5 is an enzyme solution according to any one of embodiments 1 to 4, wherein the isothiazolinone comprises at least one component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one.
• Embodiment 6 is an enzyme solution according to any one of embodiments 1 to 5, wherein the benzoisothiazolinone comprises 1,2-benzoisothiazolin-3-one.
• Embodiment 7 is an enzyme solution according to any one of embodiments 1 to 6, wherein the isothiazolinone and the benzoisothiazolinone are in a weight ratio of 3:1 to 30:1, and the isothiazolinone and the sodium benzoate are in a weight ratio of 1:1 to 30:1.
• Embodiment 8 is an enzyme solution according to any one of embodiments 1 to 7, wherein the normal-temperature enzyme stabilizer comprises at least one component selected from the group consisting of glycerol, propylene glycol, calcium chloride, magnesium chloride, and boric acid.
• the normal-temperature enzyme stabilizer comprises at least one component selected from the group consisting of glycerol, propylene glycol, calcium chloride, magnesium chloride, and boric acid.
• Embodiment 9 is an enzyme solution according to embodiment 8, wherein the glycerol and/or propylene glycol is in an amount of 5 to 10 wt.%, and the calcium chloride and/or magnesium chloride is in an amount of 0.03 to 0.7 wt.%, based on the total weight of the enzyme solution.
• Embodiment 10 is an enzyme solution according to any one of embodiments 1 to 9, wherein the solubilizer comprises at least one component selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, xylene sulfuric acid, sodium xylene sulfate, and alkyl amine oxide.
• Embodiment 11 is an enzyme solution according to any one of embodiments 1 to 10, wherein the pH adjuster comprises citric acid.
• Embodiment 12 is an enzyme solution according to any one of embodiments 1 to 11, wherein the enzyme solution further comprises 0.2 to 0.5 wt.% of a chelator, based on the total weight of the enzyme solution.
• Embodiment 13 is an enzyme solution according to embodiment 12, wherein the chelator comprises at least one component selected from the group consisting of ethylendiamine tetraacetic acid and diethylenetriamine pentaacetic acid.
• Embodiment 14 is an enzyme solution according to any one of embodiments 1 to 13, wherein the enzyme solution further comprises 0.5 to 10 wt.% of a corrosion inhibitor, based on the total weight of the enzyme solution.
• Embodiment 15 is an enzyme solution according to embodiment 14, wherein the corrosion inhibitor comprises at least one component selected from the group consisting of a borate ester, a silicate and polyaspartic acid.
• Embodiment 16 is an enzyme solution according to any one of embodiments 1 to 15, wherein the enzyme solution further comprises 0.1 to 0.3 wt.% of a defoamer, based on the total weight of the enzyme solution.
• Embodiment 17 is an enzyme solution according to embodiment 16, wherein the defoamer comprises at least one component selected from the group consisting of a silicone oil, a polyether and a higher alcohol.
• Embodiment 18 is an enzyme solution according to any one of embodiments 1 to 17, wherein the enzyme solution has a pH value of 7 to 9.
• Embodiment 19 is a method of preparing an enzyme solution, comprising the step of mixing the ingredients of the enzyme solution according to any one of embodiments 1 to 18.
• Embodiment 20 is the method according to embodiment 19, comprising the steps of sequentially adding 6.9 to 10 wt.% of a solubilizer, 0.1 to 10 wt.% of a nonionic surfactant, 0.75 to 6 wt.% of an isothiazolinone, 0.1 to 1 wt.% of a benzoisothiazolinone, 0.1 to 1 wt.% of sodium benzoate, 0.03 to 15 wt.% of a normal-temperature enzyme stabilizer into 30 to 60 wt.% of water to obtain a first solution; adding 0.15 to 0.5 wt.% of a pH adjuster to the first solution to obtain a second solution having a pH value of 6 to 10; and adding 0.01 to 1.2 wt.% of an enzyme and 3 to 51 wt.% of water into the second solution to obtain the enzyme solution.
• Embodiment 21 is the method according to embodiment 19, comprising the steps of sequentially adding 6.9 to 10 wt.% of a solubilizer, 0.1 to 10 wt.% of a nonionic surfactant, 0.75 to 6 wt.% of an isothiazolinone, 0.1 to 1 wt.% of a benzoisothiazolinone, 0.1 to 1 wt.% of sodium benzoate, 0.03 to 15 wt.% of a normal-temperature enzyme stabilizer, 0.2 to 0.5 wt.% of a chelator, 0.5 to 10 wt.% of a corrosion inhibitor, and 0.1 to 0.3 wt.% of a defoamer into 30 to 60 wt.% of water to obtain a first solution; adding 0.15 to 0.5 wt.% of a pH adjuster into the first solution to obtain a second solution having a pH value of 6 to 10; and adding 0.01 to 1.2 wt.% of an enzyme and
• Embodiment 22 is an enzyme-containing non-woven fabric, comprising a non-woven fabric and the enzyme solution according to any one of embodiments 1 to 18 dispersed in the non-woven fabric, wherein the non-woven fabric comprises 20 to 50 wt.% of viscose fiber and 50 to 80 wt.% of terylene fiber based on the total weight of the non-woven fabric; and the enzyme solution and the non-woven fabric are in a weight ratio of 1.5:1 to 7:1.
• Embodiment 23 is a method of preparing an enzyme-containing non-woven fabric, comprising the step of allowing the enzyme solution according to any one of embodiments 1 to 18 to disperse into a non-woven fabric, wherein the non-woven fabric comprises 20 to 50 wt.% of viscose fiber and 50 to 80 wt.% of terylene fiber based on the total weight of the non-woven fabric; and the enzyme solution and the non-woven fabric are in a weight ratio of 1.5:1 to 7:1.
• Embodiment 24 is use of the enzyme-containing non-woven fabric according to embodiment 22 in cleaning medical devices.
• the first solution may, as needed, further comprise 0.2 to 0.5 wt.% of a chelator, 0.5 to 10 wt.% of a corrosion inhibitor, and 0.1 to 0.3 wt.% of a defoamer, based on the total weight of the enzyme solution.
• the enzyme solution can be dispersed into the non-woven fabric by appropriate means such as sprinkling, and soaking etc. to obtain the enzyme-containing non-woven fabric.
• the cleaning capability of the enzyme solution is evaluated as per “Testing of the cleaning capability of the enzyme solution”.
• the activity of the enzyme in the enzyme-containing non-woven fabric is evaluated as per“Testing of enzyme activity”.
• the compatibility of the non-woven fabric with the enzyme solution in the enzyme-containing non-woven fabric is evaluated as per“Testing of the compatibility of the enzyme solution with the non-woven fabric”.
• the stability of the enzyme in the enzyme-containing non-woven fabric is evaluated as per“Testing of enzyme stability”.
• the mold-resistant and bacteria-resistant capabilities of the enzyme-containing non-woven fabric is evaluated as per“Microbial challenge testing”.
• enzyme-containing non-woven fabric is evaluated as per“Endoscope damage testing”.
• enzyme-containing non-woven fabric is evaluated as per“Corrosiveness testing”. Testing of the cleaning capability of the enzyme solution
• X-OMAT BT medical X-ray film Commercially available from Kodak Corporation (the X-OMAT BT medical X-ray film has the model number of XBT-1 and has gelatin on its surface) into a testing piece having a length of 8.0 ⁇ 1.0 mm and a width of 3.0 ⁇ 1.0 mm.
• thermostated water bath Adjusts a thermostated water bath to a specified temperature (37-40 oC) and maintain the temperature.
• rinsing time t is smaller than 8 minutes, then it indicates that the rinsing capability of the enzyme solution tested is acceptable. Testing of enzyme activity
• the testing of enzyme stability includes“testing of enzyme stability under normal temperature (10 to 25oC) condition” and“testing of enzyme stability under relatively high temperature (higher than 25oC) condition”.
• Enzyme solution and enzyme-containing non-woven fabric are prepared respectively according to the ingredients and amounts thereof listed in Table 2a , Table 2b and Table 2c (the amounts listed in the Table 2a , Table 2b and Table 2c are all counted by weight percentage) by the methods described above.
• the enzyme solution in the enzyme-containing non-woven fabric provided in Examples 1 to 13 has good cleaning capability.
• the non-woven fabric contains an appropriate amount of viscose fiber and terylene fiber, and the enzyme solution contains 0.75 to 6 wt.% of isothiazolinone, 0.1 to 1 wt.% of
• the non-woven fabric has good compatibility with the enzyme.
• the non-woven fabric only contains bamboo fibers, and the enzyme solution contains neither benzoisothiazolinone nor sodium benzoate, therefore the non-woven fabric is less compatible with the enzyme.
• the non-woven fabric In the enzyme-containing non-woven fabric provided in Comparative Example 2, the non-woven only contains viscose fiber, therefore the non-woven fabric is less compatible with the enzyme.
• the non-woven fabric contains an appropriate amount of viscose fiber and terylene fiber, the enzyme solution does not contain an appropriate amount of isothiazolinone, benzoisothiazolinone and sodium benzoate, therefore the non-woven fabric is less compatible with the enzyme.
• the enzyme solution contains 0.03 to 15 wt.% of normal-temperature enzyme stabilizer, therefore the enzyme solution dispersed in the non-woven fabric has good enzyme stability under normal temperature (10 to 25oC) condition.
• the enzyme solution contains 0.75 to 6 wt.% of isothiazolinone, 0.1 to 1 wt.% of benzoisothiazolinone and 0.1 to 1 wt.% of sodium benzoate, therefore the enzyme solution dispersed in the non-woven fabric has good enzyme stability under relatively high temperature (higher than 25oC) condition.
• the enzyme solution contains 0.3 wt.% of isothiazolinone, does not contain benzoisothiazolinone or sodium benzoate, therefore the enzyme solution dispersed in the non-woven fabric has insufficient enzyme stability under relatively high temperature (higher than 25oC) condition.
• the enzyme solution contains 0.5 wt.% of isothiazolinone, 0.1 wt.% of benzoisothiazolinone and 0.5 wt.% of sodium benzoate, the amount of isothiazolinone is not enough,, therefore the enzyme solution dispersed in the non-woven fabric has insufficient enzyme stability under relatively high temperature (higher than 25oC) condition.
• the enzyme solution contains 0.5 wt.% of isothiazolinone, 1 wt.% of benzoisothiazolinone and 0.5 wt.% of sodium benzoate, the amount of isothiazolinone is not enough, therefore the enzyme solution dispersed in the non-woven fabric has insufficient enzyme stability under relatively high temperature (higher than 25oC) condition.
• the enzyme solution contains 1.5 wt.% of isothiazolinone and 0.1 wt.% of benzoisothiazolinone, does not contain sodium benzoate, therefore the enzyme solution dispersed in the non-woven fabric has insufficient enzyme stability under relatively high temperature (higher than 25oC) condition.
• the enzyme solution contains 1.5 wt.% of isothiazolinone and 0.5 wt.% of sodium benzoate, does not contain benzoisothiazolinone, therefore the enzyme solution dispersed in the non-woven fabric has insufficient enzyme stability under relatively high temperature (higher than 25oC) condition.
• the enzyme solution contains0.75 to 6 wt.% of isothiazolinone, 0.1 to 1 wt.% of benzoisothiazolinone and 0.1 to 1 wt.% of sodium benzoate, therefore the enzyme solution dispersed in the non-woven fabric has good mold-resistant and bacteria-resistant properties.
• the enzyme solution contains 0.3 wt.% of isothiazolinone, does not contain benzoisothiazolinone or sodium benzoate, therefore the enzyme solution dispersed in the non-woven fabric has insufficient mold-resistant and bacteria-resistant properties.
• the enzyme solution contains 0.5 wt.% of isothiazolinone, 0.1 wt.% of benzoisothiazolinone and 0.5 wt.% of sodium benzoate, the amount of isothiazolinone is not enough,, therefore the enzyme solution dispersed in the non-woven fabric has insufficient mold-resistant and bacteria-resistant properties.
• the enzyme solution contains 0.5 wt.% of isothiazolinone, 1 wt.% of benzoisothiazolinone and 0.5 wt.% of sodium benzoate, the amount of isothiazolinone is not enough, therefore the enzyme solution dispersed in the non-woven fabric has insufficient mold-resistant and bacteria-resistant properties.
• the enzyme solution contains 1.5 wt.% of isothiazolinone and 0.1 wt.% of benzoisothiazolinone, does not contain sodium benzoate, therefore the enzyme solution dispersed in the non-woven fabric has insufficient mold-resistant and bacteria-resistant properties.
• the enzyme solution contains 1.5 wt.% of isothiazolinone and 0.5 wt.% of sodium benzoate, does not contain benzoisothiazolinone, therefore the enzyme solution dispersed in the non-woven fabric has insufficient mold-resistant and bacteria-resistant properties.
• the enzyme-containing non-woven fabric provided in Examples 1 to 9 is less likely to cause damage to medical devices.
• the enzyme-containing non-woven fabric provided in Examples 1 to 13 is less likely to cause corrosion to the metal or rubber components of medical devices.
• the enzyme solution according to the present disclosure has good cleaning capability. Moreover, the enzyme solution according to the present disclosure comprises appropriate amount of isothiazolinone, benzoisothiazolinone and sodium benzoate, therefore the enzyme solution has good compatibility with the non-woven fabric comprising viscose fiber and terylene fiber.
• the enzyme solution dispersed in the non-woven fabric not only has good enzyme stability, but also has good mold-resistant and bacteria-resistant properties. Furthermore, the enzyme-containing non-woven fabric according to the present disclosure is less likely to cause damage to medical devices, and is less likely to cause corrosion to the metal or rubber components of medical devices.

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