WO2016079820A1 - Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse - Google Patents

Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse Download PDF

Info

Publication number
WO2016079820A1
WO2016079820A1 PCT/JP2014/080574 JP2014080574W WO2016079820A1 WO 2016079820 A1 WO2016079820 A1 WO 2016079820A1 JP 2014080574 W JP2014080574 W JP 2014080574W WO 2016079820 A1 WO2016079820 A1 WO 2016079820A1
Authority
WO
WIPO (PCT)
Prior art keywords
biological reaction
porous structure
micro
microorganism
culture solution
Prior art date
Application number
PCT/JP2014/080574
Other languages
English (en)
Japanese (ja)
Inventor
信秀 国友
宏記 藤井
伸宏 田中
正守 樋口
Original Assignee
三菱化学エンジニアリング株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 三菱化学エンジニアリング株式会社 filed Critical 三菱化学エンジニアリング株式会社
Priority to PCT/JP2014/080574 priority Critical patent/WO2016079820A1/fr
Priority to JP2016560283A priority patent/JPWO2016080487A1/ja
Priority to PCT/JP2015/082548 priority patent/WO2016080487A1/fr
Publication of WO2016079820A1 publication Critical patent/WO2016079820A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/084—Polymers containing vinyl alcohol units
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof

Definitions

  • the present invention relates to a bioreactor and a bioreaction method using the bioreactor, in which a porous structure in which an aerobic microorganism is supported to the back of a pore is present in a microorganism culture solution, and the microorganism culture solution It is characterized by containing micro-nano bubbles.
  • a porous structure not supporting aerobic microorganisms is simply referred to as “porous structure”, and a porous structure supporting aerobic microorganisms is referred to as “supporting structure”.
  • the present invention also relates to a support structure and a method for producing the support structure used in the bioreaction apparatus or the bioreaction apparatus, and includes a porous structure and a culture solution containing aerobic microorganisms and micro-nano bubbles. By contacting, an aerobic microorganism is carried to the back of the pores of the porous structure.
  • Biological reactions differ from chemical reactions in that the reaction itself is slow, but because it does not use much energy and many chemical substances, it is a mild and meaningful reaction for the environment.
  • the biological reaction generally has a problem that the reaction is mild and slow. That is, for chemical reactions, a reaction within one hour is often sufficient, whereas in the case of biological reactions, a reaction time of several days or longer, several days or particularly several weeks or longer is required. There is also. For this reason, it is required to perform biological reactions efficiently and economically.
  • Patent Documents 1 to 3 disclose that in the cultivation of microorganisms, the presence of micro-nano bubbles or nano-bubbles in the culture medium promotes the activation of microorganisms and the like. It is disclosed that the efficiency and the reaction time are reduced.
  • Patent Document 1 in a batch method, a culture solution is extracted from a culture tank, filtered with a bacterial cell filter to obtain a filtrate, and micronano bubbles are mixed with the filtrate and returned to the culture tank. It is described to do.
  • Patent Document 2 describes that micro-nano bubbles and nano bubbles are mixed with the culture liquid before supplying the culture liquid to the culture tank.
  • Patent Document 3 supplies the culture liquid to the culture tank. It is described that the micro / nano bubbles are mixed in the previous stage.
  • Patent Documents 4 to 5 that a microorganism is supported by a porous carrier in a biological reaction.
  • Patent Document 4 describes that a microorganism used for wastewater treatment is attached to and supported on a polyvinyl alcohol-containing hydrogel
  • Patent Document 5 describes a citrate-fermenting bacterium.
  • treated water containing saccharides and saccharides is brought into contact with a polyvinyl alcohol-based porous gel so that citric acid-fermenting bacteria are supported on the polyvinyl alcohol-based porous gel.
  • the aerobic microorganism can be transformed into a polyvinyl alcohol-based hydrogel or polyvinyl by simply bringing the bacteria into contact with and supporting the polyvinyl alcohol-based hydrogel or polyvinyl alcohol-based porous gel. It cannot be supported to the back of the pores of the alcoholic porous gel.
  • the subject of the biological reaction apparatus of this invention and the biological reaction method using this biological reaction apparatus is the biological reaction which can perform the biological reaction using an aerobic microorganism efficiently and economically further using a micro nano bubble. It is to provide an apparatus and a biological reaction method.
  • Another object of the porous structure carrying the aerobic microorganism of the present invention and the method for producing the porous structure is used in a biological reaction apparatus or a biological reaction method, and performs a biological reaction efficiently and economically.
  • An object of the present invention is to provide a porous structure and a method for producing the porous structure.
  • the biological reaction apparatus of the present invention and the biological reaction method using this biological reaction apparatus have a supporting structure in which aerobic microorganisms are supported at a high density in a microorganism culture solution, This microorganism culture solution contains micro-nano bubbles.
  • the porous structure carrying the aerobic microorganism of the present invention is characterized in that the aerobic microorganism is carried at a high density, and the method for producing the porous structure comprises a porous structure. And an aerobic microorganism and a culture solution containing micro-nano bubbles are brought into contact with each other.
  • micro nano bubble of the present invention means “micro bubble” and / or “nano bubble”. While “normal bubbles” rapidly rise in water and burst and disappear on the surface, microbubbles with a diameter of 50 ⁇ m or less called “microbubbles” shrink in water and disappear. Together with free radicals, “nanobubbles”, which are ultrafine bubbles having a diameter of 100 nm or less, are generated, and these “nanobubbles” remain in water for a certain amount of time.
  • Nano bubbles which are very small bubbles, are also called “ultra fine bubbles”.
  • ISO International Organization for Standardization
  • the creation of an international standard for fine bubble technology is being considered, and once the international standard is created, the name of “nanobubble”, which is currently commonly used, There is a possibility that it will be unified into “Ultra Fine Bubble”.
  • micro / nano bubbles of the present invention can be formed from air or oxygen, but it is preferable to use oxygen from the viewpoint of efficiently supplying oxygen necessary for respiration to aerobic microorganisms.
  • Patent Documents 1 to 3 utilize these micro-nano bubbles and the action of nano bubbles activating microorganisms.
  • micro-nano bubbles are deep inside the pores of the porous structure. The bioreaction using aerobic microorganisms is performed efficiently and economically by utilizing the action of being able to invade even the most.
  • a porous structure having a high supporting density in which aerobic microorganisms are supported to the back of the pores using micro-nano bubbles is used.
  • the filtration step of separating the aerobic microorganisms from the microorganism culture solution and collecting the filtrate can be easily performed with a large stress on the aerobic microorganisms. It can be done efficiently and economically without causing damage.
  • the microorganism culture solution containing micro-nano bubbles since the microorganism culture solution containing micro-nano bubbles is used, the micro-nano bubbles penetrate deep into the pores of the porous structure, and the aerobic microorganisms supported in the deep pores of the porous structure. In addition, since oxygen necessary for respiration can be sufficiently supplied, the efficiency of biological reaction can be increased.
  • the main features of the biological reaction apparatus of the present invention and the biological reaction method using this biological reaction apparatus are as described above. 1) The presence of a supporting structure having a high supporting density in which aerobic microorganisms are supported at a high density in the microorganism culture solution; 2) Inclusion of micro / nano bubbles in the microorganism culture solution; However, these items will be described in turn below.
  • Support structure has a high support density in which aerobic microorganisms are supported to the depths of the pores.
  • the support structure includes a porous structure, aerobic microorganisms, and micro-nano bubbles. It can manufacture by making it contact with the culture solution containing.
  • porous structure it is preferable to use those having pores, particularly those having a three-dimensional solid network structure having continuous pores. If only a porous structure having such a structure is used, oxygen is not sufficiently supplied to the inside of the pores, so that aerobic microorganisms can only be supported on the surface of the porous structure.
  • the present invention by using a culture solution containing aerobic microorganisms and micro-nano bubbles as a culture solution to be contacted with the porous structure, oxygen can be supplied to the depths of the pores of the porous structure. It is possible to carry aerobic microorganisms to the back of the substrate, and to increase the carrying density of aerobic microorganisms.
  • target product such as a metabolite of an aerobic microorganism obtained by a biological reaction from a microorganism culture tank
  • target product a reaction product such as a metabolite of an aerobic microorganism obtained by a biological reaction
  • the aerobic microorganism is separated from the microorganism culture solution.
  • the aerobic microorganisms are supported by the porous structure, the aerobic microorganisms can be separated easily and economically.
  • aerobic microorganisms are added to a culture solution, and after aerobic microorganisms are cultured and grown, they are brought into contact with and supported by the porous structure, or culture.
  • Examples include a method in which an aerobic microorganism and a porous structure are added to the liquid, and the aerobic microorganism is supported while being cultured and grown.
  • the former method is performed by the following procedure. a) First, sterilize the porous structure, the culture solution, and the like so that microorganisms, bacteria, and the like other than the required aerobic microorganisms do not exist. b) Next, aerobic microorganisms are added to the culture solution, and the aerobic microorganisms are cultured and grown while the culture solution contains micro-nano bubbles. c) At the stage where aerobic microorganisms have grown to a certain concentration or higher, the medium is brought into contact with the porous structure while containing micro-nano bubbles in the culture solution.
  • the latter method is performed in the following procedure. a) First, sterilize the porous structure, the culture solution, and the like so that microorganisms, bacteria, and the like other than the required aerobic microorganisms do not exist. d) Next, the porous structure is added to the culture solution, and the culture solution contains micro-nano bubbles. e) Add aerobic microorganisms to the culture solution, and culture and proliferate the aerobic microorganisms while containing micro-nano bubbles in the culture solution.
  • the culture solution used in the above method mainly contains saccharides and a nitrogen source.
  • saccharides saccharides such as maltose, sucrose, glucose, fructose, and mixtures thereof are usually used.
  • concentration of saccharides in the culture solution is not particularly limited, but is set to 0.1 to 10 w / v%. preferable.
  • nitrogen source ammonium chloride, ammonium sulfate, corn steep liquor, yeast extract, meat extract, peptone or the like is used, and it is preferably set to 0.1 to 10 w / v%.
  • vitamins, inorganic salts, and the like to the culture solution as needed in addition to the saccharides and the nitrogen source.
  • the concentration of the aerobic microorganism added to the culture solution in step b) and step e) is not particularly limited, but is preferably 0.5 to 10 g / L, and preferably 3.0 to 6.0 g / L. Is more preferable.
  • micronano bubbles are contained in the treated water in order to promote the growth of aerobic microorganisms.
  • the culture and proliferation of aerobic microorganisms can be carried out sufficiently rapidly without the inclusion of micronano bubbles. If possible, the inclusion of micro-nano bubbles can be omitted.
  • the contact between the culture solution containing the micro-nano bubbles and the porous structure can be performed, for example, by placing the porous structure in the treated water and stirring with shaking.
  • aerobic microorganism in the present invention, aspergillus oryzae (5 to 10 ⁇ m), Bacillus natto (2 to 3 ⁇ m), acetic acid bacteria (5 to 10 ⁇ m), which are conventionally used in technical fields such as brewing and fermentation,
  • aerobic microorganisms such as yeast (5 ⁇ m) and lactic acid bacteria (2 to 4 ⁇ m)
  • various aerobic microorganisms created by genetic engineering techniques can be used.
  • the pore diameter of the continuous pores of the porous structure having a three-dimensional three-dimensional network structure having continuous pores is preferably 1 to 30 ⁇ m, although it depends on the size of the aerobic microorganism to be supported, usually around 5 to 10 ⁇ m. It is more preferable that
  • Examples of the material of the porous structure suitably used in the present invention include vinyl alcohol resins such as polyvinyl alcohol, ether resins such as polyethylene glycol, acrylic resins such as polymethacrylic acid, acrylamide resins such as polyacrylamide, polyethylene, and polypropylene.
  • vinyl alcohol resins such as polyvinyl alcohol
  • ether resins such as polyethylene glycol
  • acrylic resins such as polymethacrylic acid
  • acrylamide resins such as polyacrylamide, polyethylene, and polypropylene.
  • Olefin resins such as polystyrene, ester resins such as polyethylene terephthalate and polybutylene terephthalate, acrylonitrile resins such as polyacrylonitrile, urethane resins such as polyurethane sponge, calcium alginate, ⁇ (kappa) carrageenan, agar, and cellulose derivatives Sugar, polyester acrylate, epoxy acrylate, urethane acrylate It said photocurable resin, and the like can be exemplified porous inorganic compounds such as activated carbon.
  • a polyvinyl alcohol-based porous gel is preferable in that it has a porous and mesh-like structure up to the inside and a large amount of water can be taken into the gel.
  • the mechanical strength of the porous gel can be improved sufficiently, and it has the strength that it can withstand even with strong agitation during a biological reaction.
  • Formalized polyvinyl alcohol porous gel and acetalization A polyvinyl alcohol-based porous gel is more preferable.
  • Specific examples of the polyvinyl alcohol-based porous gel include, for example, Kuraray Co., Ltd. trade name.
  • the carrying structure of the present invention is one in which the carrying density of aerobic microorganisms is increased by adding micro-nano bubbles to the culture solution.
  • An element for efficiently carrying out a biological reaction is to increase the cell density of aerobic microorganisms in the microorganism culture solution, but if the cell density is too high, nutrients and oxygen are added to the aerobic microorganisms. Since it is not provided sufficiently, the efficiency of the biological reaction is reduced.
  • the cell density at which aerobic microorganisms can be cultured properly is about 3 to 6 g / L.
  • the carrying structure of the present invention by containing micro-nano bubbles in the culture solution, aerobic microorganisms can be carried deep into the pores of the porous structure.
  • the cell density can be about 5 to 6 times the cell density of the culture medium.
  • the shape of the supporting structure is not particularly limited, but for example, a granular shape such as a spherical shape, a rectangular parallelepiped shape or a cubic shape is preferable. If the powder is used, the surface area for aerobic microorganism immobilization can be greatly increased, and the target product can be produced with higher efficiency.
  • the particle size (diameter) of the porous gel powder when dried is preferably 0.5 to 10 mm.
  • the usage structure of the supporting structure may be fixed in the microorganism culture solution with a column, a net or the like, or may be present in a dispersed state in the microorganism culture solution.
  • the support structure obtained in 1) above is housed in a microorganism culture tank together with the culture solution, and the microorganism culture solution contains micro-nano bubbles. Biological reactions take place.
  • a porous structure having a high aerobic microorganism loading density in which aerobic microorganisms are carried to the back of the pores is used for the biological reaction.
  • oxygen necessary for respiration can be sufficiently supplied to the aerobic microorganisms supported in the back of the pores of the porous structure, so that the efficiency of the biological reaction can be improved.
  • the means with the least stress and damage to the aerobic microorganism are the means b) and d) above.
  • aerobic microorganisms are stressed and damaged by the shearing force generated by the release of nanobubbles in the means a) and by the shearing force generated during filtration in the c) means. Then, since the aerobic microorganisms are supported by the porous structure, this stress and damage can be greatly reduced.
  • micro / nano bubble generating device a known or commercially available device can be used.
  • microbubble generator for example, a sufficient amount of gas is dissolved in water at a certain high pressure, and then the pressure is released to create a supersaturated condition of the dissolved gas.
  • a gas-liquid two-phase flow swirl type micro-bubble generator that utilizes the phenomenon of generating a vortex by generating a water flow, entraining a large bubble in the vortex, and then breaking the vortex into bubbles. Or the like.
  • nanobubble generators include, for example, JP 2007-312690, JP 2006-289183, JP 2005-245817, JP 2007-136255, and JP 2009-39600. Those described can be used.
  • the recovery of the target product may be performed batchwise or continuously.
  • the culture tank pump is driven to transfer the filtered filtrate to the filtrate storage tank.
  • the batch type or the continuous type can be selected as appropriate in consideration of the efficiency of biological reaction, the required purity of the target product, economy, and the like.
  • filtration can be performed efficiently and economically, and further, stress and damage given to aerobic microorganisms can be reduced.
  • a porous membrane made of an organic polymer compound such as polyvinylidene fluoride, or a filtration membrane such as a metal wire mesh can be suitably used.
  • the biological reaction apparatus of the present invention and the biological reaction method using this biological reaction apparatus can efficiently and economically perform biological reactions using aerobic microorganisms using micro-nano bubbles. It can be applied not only to the production of foods, chemicals, chemicals and the like using biological reactions such as brewing and fermentation, but also to a biorefinery for producing bioethanol and the like using biomass.
  • porous structure carrying the aerobic microorganism of the present invention is useful for performing a biological reaction efficiently and economically.
  • FIG. 1 schematically shows a first embodiment of the biological reaction apparatus of the present invention.
  • -The carrying structure 7 is fixed in the microorganism culture tank 1 by the fixing member 8;
  • O The micro / nano bubble generating device 3 is provided in a pipeline for supplying the culture solution 6 to the microorganism culture tank 1, and the culture solution 6 supplied to the microorganism culture tank 1 contains micro / nano bubbles.
  • a micro-nano bubble generator 2 is provided outside the microorganism culture tank 1, and the micro-nano bubbles are blown into the microorganism culture solution 5 of the microorganism culture tank 1, It is characterized by.
  • the target product is generated and collected by the following steps. a) When supplying to the microorganism culture tank 1, the micro / nano bubble generator 3 causes the culture solution 6 to contain micro / nano bubbles. b) Even after supplying to the microorganism culture tank 1, the micro / nano bubbles are blown from the micro / nano bubble generator 2 to cause the microorganism culture solution 5 to contain the micro / nano bubbles. c) In the microorganism culture tank 1, a target product is generated by a biological reaction of aerobic microorganisms supported on the support structure 7. d) The culture tank pump 10 is driven, the supporting structure 7 detached from the fixing member 8 is separated by the filtering means 9, and the filtrate containing the target substance is collected in the filtrate storage tank 13.
  • the supporting structure 7 having aerobic microorganisms supported to the back of the pores and having a high aerobic microorganism supporting density, and the microorganism culture solution 5 containing micro-nano bubbles.
  • the biological reaction can be advanced efficiently, and the target product can be obtained efficiently.
  • FIG. 2 schematically shows a second embodiment of the biological reaction apparatus of the present invention.
  • the means for causing the culture solution 6 and the microorganism culture solution 5 to contain micro-nano bubbles in the first embodiment, and the micro-nano bubbles are contained in the filtrate filtered by the filtration means 9 using the micro-nano bubble generator 4.
  • the means for refluxing the filtrate containing the micro / nano bubbles to the microorganism culture tank 1 is used.
  • the target product is generated and collected by the following steps. a) The culture solution 6 is supplied to the microorganism culture tank 1. b) The culture tank pump 10 is driven with the valve 11 opened and the valve 12 closed, the carrier structure 7 detached from the fixing member 8 is separated by the filtering means 9, and the filtrate is supplied to the micro / nano bubble generator 4. Then, after containing micro-nano bubbles, it is returned to the microorganism culture tank 1. c) In the microorganism culture tank 1, a target product is generated by a biological reaction of aerobic microorganisms supported on the support structure 7.
  • the culture tank pump 10 is driven in a state in which the valve 11 is closed and the valve 12 is opened, and the supporting structure 7 detached from the fixing member 8 is separated by the filtering means 9 and filtered containing the target product. Water is collected in the filtrate storage tank 13.
  • the means for incorporating the micro-nano bubbles into the filtrate of the second embodiment is applied to the filtrate at a location different from the microorganism culture tank 1, so that the aerobic microorganisms are subjected to stress or damage due to shearing force or the like. There is an advantage that it is difficult.
  • FIG. 3 schematically shows a third embodiment of the biological reaction apparatus of the present invention.
  • Means for containing micro-nano bubbles in the culture solution 6 or the microorganism culture solution 5 Means for containing micro-nano bubbles in the culture medium 6 supplied to the microorganism culture tank 1 and the microorganism culture liquid 5 in the microorganism culture tank 1 used in the first embodiment, and the filtration used in the second embodiment
  • the filtrate filtered by the means 9 is made to contain micro-nano bubbles, and the means for refluxing the filtrate containing the micro-nano bubbles to the microorganism culture tank 1 is used in combination.
  • FIG. 4 schematically shows a fourth embodiment of the biological reaction apparatus of the present invention.
  • the support structure 7 is present in a dispersed state in the microorganism culture solution 5, and the microorganism culture vessel 1 further includes a culture vessel agitator 14 for stirring the microorganism culture solution 5. is set up.
  • the carrier structure 7 is present in a dispersed state in the microorganism culture tank 1 and is stirred by the culture tank agitator 14;
  • the micro / nano bubble generating device 3 is provided in a pipeline for supplying the culture solution 6 to the microorganism culture tank 1, and the culture solution 6 supplied to the microorganism culture tank 1 contains micro / nano bubbles.
  • a micro-nano bubble generator 2 is provided outside the microorganism culture tank 1, and the micro-nano bubbles are blown into the microorganism culture solution 5 of the microorganism culture tank 1, It is characterized by.
  • the object is generated and collected by the following steps. a) When supplying to the microorganism culture tank 1, the micro / nano bubble generator 3 causes the culture solution 6 to contain micro / nano bubbles. b) Even after supplying to the microorganism culture tank 1, the micro / nano bubbles are blown from the micro / nano bubble generator 2 to cause the microorganism culture solution 5 to contain the micro / nano bubbles. c) In the microorganism culture tank 1, a target product is generated by the biological reaction of the aerobic microorganisms supported on the support structure 7 to be stirred. d) The culture tank pump 10 is driven, the supporting structure 7 is separated by the filtering means 9, and the filtrate containing the target substance is collected in the filtrate storage tank 13.
  • the supporting structure 7 having a high aerobic microorganism supporting density and supporting the aerobic microorganism to the back of the pores and the microorganism culture solution 5 containing the micro / nano bubbles.
  • the biological reaction can be advanced efficiently, and the target product can be obtained efficiently.
  • the support structure 7 is dispersed and stirred in the microorganism culture solution 5, nutrients and oxygen are sufficiently provided also to the aerobic microorganisms supported in the back of the pores of the porous structure.
  • a biological reaction can be promoted, there is a demerit such that the aerobic microorganisms are easily subjected to stress and damage due to the shearing force by stirring, and the aerobic microorganisms are easily detached from the porous structure.
  • FIG. 5 schematically shows a fifth embodiment of the biological reaction apparatus of the present invention.
  • the means for causing the culture solution 6 and the microorganism culture solution 5 to contain micro-nano bubbles in the fourth embodiment, and the micro-nano bubbles are contained in the filtrate filtered by the filtration means 9 using the micro-nano bubble generator 4.
  • the means for refluxing the filtrate containing the micro / nano bubbles to the microorganism culture tank 1 is used.
  • the target object is generated and collected by the following steps.
  • a) The culture solution 6 is supplied to the microorganism culture tank 1.
  • the culture tank pump 10 is driven with the valve 11 opened and the valve 12 closed, the supporting structure 7 is separated by the filtering means 9, the filtrate is guided to the micro / nano bubble generator 4, and contains micro / nano bubbles Then, return to the microorganism culture tank 1.
  • a target product is generated by the biological reaction of the aerobic microorganisms supported on the support structure 7 to be stirred.
  • the culture tank pump 10 is driven, the supporting structure 7 is separated by the filtering means 9, and the filtrate containing the target substance is collected in the filtrate storage tank 13.
  • the biological reaction can be promoted.
  • the aerobic microorganisms are easily subjected to stress and damage due to the shearing force by stirring, and the aerobic microorganisms have a porous structure. There are disadvantages such as being easy to leave the body.
  • the means for adding micro-nano bubbles to the filtrate is applied to the filtrate at a location different from the microorganism culture tank 1, so that the aerobic microorganisms are subjected to stress caused by shearing force or the like. There is an advantage that it is not easily damaged.
  • FIG. 6 schematically shows a sixth embodiment of the biological reaction apparatus of the present invention.
  • Means for containing micro-nano bubbles in the culture solution 6 or the microorganism culture solution 5 Means for containing micro-nano bubbles in the culture medium 6 supplied to the microorganism culture tank 1 and the microorganism culture liquid 5 in the microorganism culture tank 1 used in the fourth embodiment, and the filtration used in the fifth embodiment
  • the filtrate filtered by the means 9 is made to contain micro-nano bubbles, and the means for refluxing the filtrate containing the micro-nano bubbles to the microorganism culture tank 1 is used in combination.

Abstract

Le dispositif de réaction biologique selon l'invention et le procédé de réaction biologique mettant en œuvre ce dispositif de réaction biologique traitent du problème d'une utilisation plus efficace de micro-nano-bulles et donc de l'exécution efficace et économique d'une réaction biologique à l'aide d'un micro-organisme aérobie. La structure poreuse porteuse de micro-organisme aérobie selon la présente invention, et le procédé de fabrication de cette structure poreuse traitent du problème de la fourniture d'une structure poreuse à utiliser dans un dispositif de réaction biologique ou dans un procédé de réaction biologique, ladite structure poreuse permettant la réalisation efficace et économique d'une réaction biologique, ainsi que d'un procédé de production de la structure poreuse. Pour résoudre ces problèmes, le dispositif de réaction biologique selon la présente invention et le procédé de réaction biologique mettant en œuvre ce dispositif de réaction biologique sont caractérisés en ce qu'une structure poreuse, qui porte en profondeur dans ses pores un micro-organisme aérobie à une densité portée élevée, est disposée dans un milieu de culture liquide pour micro-organisme et en ce qu'en même temps, ce milieu de culture liquide de micro-organisme contient des micro-nano-bulles. La structure poreuse porteuse du micro-organisme aérobie selon la présente invention est caractérisée en ce qu'elle porte un micro-organisme aérobie à une densité portée élevée. Le procédé de fabrication de la structure poreuse est caractérisé en ce qu'il consiste à mettre en contact une structure poreuse avec un milieu de culture liquide qui contient un micro-organisme aérobie et des micro-nano-bulles.
PCT/JP2014/080574 2014-11-19 2014-11-19 Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse WO2016079820A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/JP2014/080574 WO2016079820A1 (fr) 2014-11-19 2014-11-19 Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse
JP2016560283A JPWO2016080487A1 (ja) 2014-11-19 2015-11-19 生物反応装置、生物反応方法、並びに、生物反応装置において使用される好気性もしくは通性嫌気性微生物を担持させた多孔質構造体及びこの多孔質構造体の製造方法
PCT/JP2015/082548 WO2016080487A1 (fr) 2014-11-19 2015-11-19 Appareil de réaction biologique, procédé de réaction biologique, structure poreuse soutenant un micro-organisme aérobie ou éventuellement anaérobie utilisée dans l'appareil de réaction biologique et procédé permettant la fabrication de la structure poreuse

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2014/080574 WO2016079820A1 (fr) 2014-11-19 2014-11-19 Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse

Publications (1)

Publication Number Publication Date
WO2016079820A1 true WO2016079820A1 (fr) 2016-05-26

Family

ID=56013433

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/JP2014/080574 WO2016079820A1 (fr) 2014-11-19 2014-11-19 Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse
PCT/JP2015/082548 WO2016080487A1 (fr) 2014-11-19 2015-11-19 Appareil de réaction biologique, procédé de réaction biologique, structure poreuse soutenant un micro-organisme aérobie ou éventuellement anaérobie utilisée dans l'appareil de réaction biologique et procédé permettant la fabrication de la structure poreuse

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/JP2015/082548 WO2016080487A1 (fr) 2014-11-19 2015-11-19 Appareil de réaction biologique, procédé de réaction biologique, structure poreuse soutenant un micro-organisme aérobie ou éventuellement anaérobie utilisée dans l'appareil de réaction biologique et procédé permettant la fabrication de la structure poreuse

Country Status (2)

Country Link
JP (1) JPWO2016080487A1 (fr)
WO (2) WO2016079820A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102399749B1 (ko) * 2021-12-14 2022-05-20 (주)해인시스템 초미세기포 발생장치 및 이를 이용한 정수시스템

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05503848A (ja) * 1990-02-01 1993-06-24 アクゾ・エヌ・ヴエー 細胞の培養方法
US5534143A (en) * 1990-09-18 1996-07-09 Louisiana State University Board Of Supervisors, A Governing Body Of Louisiana State University Agricultural And Mechanical College Microbubble generator for the transfer of oxygen to microbial inocula, and microbubble generator immobilized cell reactor
JPH0937766A (ja) * 1995-07-28 1997-02-10 Kansai Paint Co Ltd 界面バイオリアクターシステム
JP2007312689A (ja) * 2006-05-26 2007-12-06 Sharp Corp 生物反応方法および生物反応装置
JP2007312690A (ja) * 2006-05-26 2007-12-06 Sharp Corp 微生物活性化方法および微生物活性化装置
US20100076380A1 (en) * 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
JP2011120535A (ja) * 2009-12-11 2011-06-23 Ihi Corp 付着性細胞培養装置
US20110177564A1 (en) * 2010-01-15 2011-07-21 Massachusetts Institute Of Technology Bioprocess and microbe engineering for total carbon utilization in biofuel production

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3091758B1 (ja) * 1999-10-15 2000-09-25 アクアス株式会社 油脂分解菌及びそれを用いる油脂含有排水の処理方法
JP2002363279A (ja) * 2001-06-01 2002-12-18 Nagase & Co Ltd ポリ−γ−グルタミン酸の製造方法
JP4879925B2 (ja) * 2008-02-25 2012-02-22 シャープ株式会社 水処理装置および水処理方法

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05503848A (ja) * 1990-02-01 1993-06-24 アクゾ・エヌ・ヴエー 細胞の培養方法
US5534143A (en) * 1990-09-18 1996-07-09 Louisiana State University Board Of Supervisors, A Governing Body Of Louisiana State University Agricultural And Mechanical College Microbubble generator for the transfer of oxygen to microbial inocula, and microbubble generator immobilized cell reactor
JPH0937766A (ja) * 1995-07-28 1997-02-10 Kansai Paint Co Ltd 界面バイオリアクターシステム
JP2007312689A (ja) * 2006-05-26 2007-12-06 Sharp Corp 生物反応方法および生物反応装置
JP2007312690A (ja) * 2006-05-26 2007-12-06 Sharp Corp 微生物活性化方法および微生物活性化装置
US20100076380A1 (en) * 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
JP2011120535A (ja) * 2009-12-11 2011-06-23 Ihi Corp 付着性細胞培養装置
US20110177564A1 (en) * 2010-01-15 2011-07-21 Massachusetts Institute Of Technology Bioprocess and microbe engineering for total carbon utilization in biofuel production

Also Published As

Publication number Publication date
WO2016080487A1 (fr) 2016-05-26
JPWO2016080487A1 (ja) 2017-08-31

Similar Documents

Publication Publication Date Title
JP4146476B2 (ja) 生物反応装置
Margaritis et al. Advances in ethanol production using immobilized cell systems
JP5639098B2 (ja) 膜生物反応器
JP5374044B2 (ja) 水素発酵装置及び水素の製造方法
WO2016117599A1 (fr) Bioréacteur doté d'un dispositif pour fournir des micro/nano-bulles contenant de l'oxygène et des micro/nano-bulles contenant un gaz microbicide tel que l'ozone
CN103865792B (zh) 一种循环式微生物发酵反应与料液分离一体化设备
JP4956052B2 (ja) 微生物活性化装置
JP2014024032A (ja) 嫌気性生物処理方法および嫌気性生物処理装置
JP2005152763A (ja) 超微細気泡の混在するリアクター用気液混合溶液と、その製造方法と、ケミカルリアクター装置と、バイオリアクター装置。
WO2014046205A1 (fr) Procédé de culture de micro-algues, et équipement de culture de micro-algues
JP6738626B2 (ja) 生物反応装置及びこの生物反応装置を用いた生物反応方法
JP2012076001A (ja) 嫌気性排水処理装置
CN114806810A (zh) 一种氧气微纳气泡增强好氧发酵生物反应器及其应用
WO2016079820A1 (fr) Dispositif de réaction biologique, procédé de réaction biologique, structure poreuse porteuse de micro-organisme aérobie à utiliser dans le dispositif de réaction biologique, et procédé de production de structure poreuse
CN102127505A (zh) 固定化细胞生物反应器
JP6499203B2 (ja) 酸素含有気体のマイクロナノバブルを供給する装置及び溶存二酸化炭素を除去する装置を備えた生物反応装置及びこの生物反応装置を用いた生物反応方法
JP6087476B1 (ja) 酸素富化マイクロナノバブルを用いた生物反応装置及びこの生物反応装置を用いた生物反応方法
JP2012076000A (ja) 一槽式嫌気性排水処理装置
US9534236B2 (en) Membranes for wastewater-generated energy and gas
JP2009261287A (ja) クロレラ・水素生産方法およびクロレラ・水素生産装置
CN113980781A (zh) 一种潮汐式二氧化碳生物甲烷化装置及方法
KR20190081764A (ko) 상하 대칭 개방형 미세조류 배양기
TWI376416B (en) Method for screening and immobilizing microorganism and immobilization microorganism particle
David et al. Cyanobacterial biofilms in natural and synthetic environments
Jalil et al. Bioreactor Design Selection for Biohydrogen Production Using Immobilized Cell Culture System

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14906642

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: JP

122 Ep: pct application non-entry in european phase

Ref document number: 14906642

Country of ref document: EP

Kind code of ref document: A1