WO2016059141A1 - Dispositif et procédé de séparation de cellules - Google Patents

Dispositif et procédé de séparation de cellules Download PDF

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Publication number
WO2016059141A1
WO2016059141A1 PCT/EP2015/073848 EP2015073848W WO2016059141A1 WO 2016059141 A1 WO2016059141 A1 WO 2016059141A1 EP 2015073848 W EP2015073848 W EP 2015073848W WO 2016059141 A1 WO2016059141 A1 WO 2016059141A1
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WO
WIPO (PCT)
Prior art keywords
insert
tube
disc
cells
insert according
Prior art date
Application number
PCT/EP2015/073848
Other languages
English (en)
Inventor
Michelle Louise JANAS
Michael John Smith
Original Assignee
Ge Healthcare Uk Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Uk Limited filed Critical Ge Healthcare Uk Limited
Priority to EP15780879.1A priority Critical patent/EP3206793A1/fr
Priority to US15/514,381 priority patent/US10737262B2/en
Priority to JP2017518452A priority patent/JP6652967B2/ja
Priority to CN201580055640.5A priority patent/CN106796216B/zh
Publication of WO2016059141A1 publication Critical patent/WO2016059141A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls

Definitions

  • the invention relates to devices, methods and kits for separating biological materials, particularly populations of cells of different densities.
  • the invention finds particular utility in the separation of blood into its different component parts or cellular populations.
  • density gradients are commonly used to separate blood into distinct fractions such as red blood cells, white blood cells and plasma.
  • the blood is overlayered onto a medium of defined density (e.g. FicollTM, Ficoll-PaqueTM, GE Healthcare) and then centrifuged. During the centrifugation process, differential migration of the blood components occurs, resulting in the formation of layers of each blood fraction. Red blood cells migrate through the Ficoll layer and sediment at the bottom of the tube while white blood cells move to the interface above the Ficoll and below the plasma layers.
  • White blood cells nominally comprise less than 1% of peripheral blood and this technique allows for their effective and facile purification.
  • the interface between the density gradient medium and the blood sample must be as sharp as possible, with minimal mixing. This can be difficult to achieve and a skilled, steady hand and patience are required. As such the loading of blood onto a density gradient medium prior to centrifugation is very time consuming. The interface is also fragile and if the tube is knocked the interface can easily mix and be destroyed.
  • WO2012/ 149641 (Stem Cell Technologies Inc.) describes an insert for a centrifuge tube which aids density gradient separation of different cellular populations present in a sample.
  • the insert is sized to fit into a centrifuge tube, and has a member which is positioned or stabilized within the tube by a support, typically a cylindrical support, thereby dividing the tube into a top and bottom portion.
  • the member is typically of a concave configuration and has at least two openings, one of which is closer to the bottom end of the tube when the insert is in position and acts to allow liquid to pass through it to the bottom portion, the second acting to allow air to escape to equalize pressure.
  • Centrifuge tubes incorporating such inserts are commercially available, i.e. SepMateTM from Stem Cell Technologies Inc.
  • AccuspinTM tubes available from Sigma- Aldrich, are designed for use with density gradient medium, such as Histopaque®, for the isolation of lymphocytes and other mononuclear cells.
  • the Accuspin tube also known as a LeucosepTM tube available from Greiner Bio-One
  • the density gradient medium such as
  • Histopaque-1077 is added to the lower chamber below the frit.
  • the blood sample is added to the top chamber and the tube centrifuged. On centrifugation, the red blood cells sediment to the bottom of the tube containing the density gradient medium, while mononuclear cells such as lymphocytes and monocytes form a dense band at the plasma/Histopaque-1077 interface. This band can then be removed by decanting or with a pipette. Contamination with red blood cells is avoided due to the barrier between the chambers.
  • FloatiesTM are small autoclavable polymer blend beads which are designed for the in vitro isolation of peripheral blood mononuclear cells (PBMC's) from human whole blood and cord blood samples by density gradient centrifugation.
  • PBMC's peripheral blood mononuclear cells
  • the beads are poured directly onto the top of the density gradient medium in a centrifuge tube and the blood sample gently added. The majority of the beads rise to the top of the sample which may then be centrifuged in the tube.
  • the PBMC's form a layer or band near the plasma-density gradient medium interface, and are collected by inserting a pipette through the layer of beads.
  • an insert (100) for a centrifuge tube comprising a disc (20) sized to fit into a centrifuge tube for dividing said tube into an upper portion and a lower portion; said disc (20) comprising a convex upper surface (21) and an outer edge (22) having one or more indentations (24) therein; and a prop (30) extending from a lower surface (26) of the disc (20) for contacting the base of said tube when the insert (100) is positioned within the tube.
  • the one or more indentations (24) allow fluid communication between the upper portion and the lower portion of the tube.
  • the one or more indentations (24) are sized to create a surface tension across the one or more indentations (24) to restrict the flow of liquid therethrough in the absence of a centrifugal force.
  • the upper surface (21) additionally comprises one or more grooves (28) connected to said one or more indentations (24).
  • the upper surface (21) of the disc (20) additionally comprises a gripping element (40).
  • the gripping element (40) is in the centre of the upper surface (21) of the disc (20).
  • the gripping element (40) is in the form of a rod.
  • the one or more groves (26) connect the centre of the disc (20) with the one or more indentations (24).
  • the grooves (26) are fan shaped, being narrower at the centre of the disc (20) than at the indentations (24).
  • lower surface (28) of the disc (20) is convex in form.
  • the one or more indentations (24) are semi-circular or oval in form.
  • the insert is composed of a polymer.
  • the polymer is an inert polymer.
  • the polymer is sterilisable, for example by autoclaving, chemical treatment or by irradiation with suitable energy such as T rays.
  • the polymer is a plastic polymer such as polyethylene or polypropylene.
  • other inert plastic polymers may be used such as polycarbonate, polyvinyl and acrylate polymers.
  • the insert has been treated to minimize microbial contamination. Examples of suitable methods include autoclaving, chemical treatment with disinfectants or antimicrobial agents, and/or irradiation with suitable energy such as T rays.
  • a centrifuge tube comprising an insert as hereinbefore described.
  • the centrifuge tube may additionally contain a density gradient medium.
  • a method for separating cells comprising the steps of. i) adding a volume of density gradient medium to a centrifuge tube; ii) positioning an insert as hereinbefore described on the surface of said density gradient medium such that the prop is in contact with the base of said tube; iii) dispensing a liquid sample containing one or more cells over the upper surface of the disc to form an interface between the density gradient medium and the sample; and iv) centrifuging the tube to separate said one or more cells from the sample.
  • the cells are selected from the group consisting of mammalian cells, red blood cells, white blood cells and stem cells.
  • the sample comprises whole blood.
  • the method additionally comprises the step of recovering the one or more cells.
  • kits comprising an insert as hereinbefore described and a centrifuge tube.
  • the kit additionally comprises a volume of density gradient medium.
  • an insert as hereinbefore described and a volume of density gradient medium there is provided an insert as hereinbefore described and a volume of density gradient medium.
  • centrifuge tubes are tubes which can be centrifuged. The tubes may have conical bottom ends which are common in the art or may be flat or round-bottomed. Examples of centrifuge tubes which are commercially available include, but are not limited to, NuncTM conical sterile polypropylene centrifuge tubes (Thermo ScientificTM), NalgeneTM Oak Ridge polycarbonate, polypropylene or TeflonTM tubes (Thermo ScientificTM), and Sterilin polypropylene tubes (Camlab, UK).
  • the insert according to the invention can be used with centrifuge tubes of varying volumes, such as for example, 2 ml, 5 ml, 10 ml, 15 ml and 50 ml.
  • the "cell” used in the present invention is a mammalian cell, preferably a human cell.
  • mammalian cells that may be used in the present invention include but are not limited to blood cells, such as red blood cells (erythrocytes), white blood cells (including leukocytes, lymphocytes, granulocytes and monocytes), platelets (thrombocytes) and stem cells (e.g. hematopoietic stem cells).
  • the "cell” may be a bone marrow cell.
  • stem cells as used herein concerns pluripotent or multipotent stem cells.
  • stem cells include, but are not limited to, embryonic stem cells (ESC), adult stem cells, haematopoietic stem cells, neural stem cells, mesenchymal stem cells and induced pluripotent stem cells(iPS).
  • ESC embryonic stem cells
  • iPS induced pluripotent stem cells
  • Figure 1 shows a three dimensional perspective view of an embodiment of an insert according to the invention.
  • Figures 2a to c depict a series of views of the insert of Figure 1 : a plan perspective (Figure 2a), a front perspective (Figure 2b), and an underside plan perspective (Figure 2c).
  • Figure 3a is a schematic diagram showing a plan perspective of an insert according to the invention with detailed inset of a groove ( Figure 3b).
  • Figures 4a, b and c are schematic diagrams of a portion of an inset according to the invention.
  • Figure 4a details the flow of a liquid over the upper surface of the disc of the insert
  • Figure 4b the height of the rise
  • Figure 4c the depth of the groove.
  • Figure 5 a), b) and c) are schematic representations of an insert according with the invention positioned within an empty centrifuge tube (Figure 5a) and a filled centrifuge tube (Figure 5b) prior to centrifugation, and following centrifugation ( Figure 5c).
  • Figure 6 is a photograph of three centrifuge tubes (550a, 550b and 550c) which have been loaded with a Ficoll-Paque density gradient medium (lower layer, 560a, b and c) and a blood/PBS mix (upper layer, 562 a, b and c) prior to separation by centrifugation.
  • a Ficoll-Paque density gradient medium lower layer, 560a, b and c
  • a blood/PBS mix upper layer, 562 a, b and c
  • Figure 7 is a photograph of the three centrifuge tubes shown in Figure 6 following centrifugation and the separation process.
  • Figure 8 is a graphical representation of the results from the flow cytometry analysis of the white blood cell fraction from each of the tubes (A corresponds to 650a, B to 650b and C to 650c) of Figure 7, the letters G indicating the Granulocyte sub-population, M the Monocyte sub-population and L the Lymphocyte sub-population present in the white blood cell fractions.
  • FSC and SSC signify forward scatter and side scatter, respectively.
  • the insert (100) consists of a disc (20) which has a diameter which is dimensioned or sized to fit into a centrifuge tube (not shown) such that once it is in position within the tube it will divide the tube into an upper and lower portion.
  • the insert can be sized to fit into any centrifuge tube, including those of standard volumes such as 10, 15 or 50 ml which are fabricated from suitable materials such as plastic, metal or glass.
  • the insert acts to minimize the mixing of liquids of different densities which occurs when a liquid containing a target (such as blood cells) to be separated into another liquid is added to a first liquid of different density as this leads to poor separation of the target into the first liquid on centrifugation.
  • the insert also facilitates the separation of the target into the first liquid during the centrifugation process.
  • the first liquid is a density gradient medium and the liquid containing the target is an aqueous blood sample.
  • the disc (20) has an upper surface (21) which is convex in form and has a plurality of indentations (24) in its outer edge (22) or circumference; the indentations can take any form but are generally curved, semi-circular or oval in shape.
  • a prop (30) extending from the lower surface (not shown) of the disc (20) acts as a leg to support the disc (20) above the base of a centrifuge tube (not shown) when the insert (100) is positioned within the tube.
  • the length of prop (30) is sized dependent on the volume of liquid to be added to the centrifuge tube, such that when the insert is added to this volume of liquid the underside of the disc will be in contact with the meniscus of the liquid.
  • a gripping element (40) facilitates positioning and removal of the insert (100) within the centrifuge tube.
  • the insert may be positioned and/or removed from a centrifuge tube manually, for example by use of an operator's fingers or a suitable tool such as a pair of forceps, or by automated or robotic means.
  • Grooves (28) connect the centre of the disc (20) with the indentations (24).
  • the purpose of the grooves (28) is to channel liquid, which may be poured onto the convex upper surface (21) of the disc (20), to the indentations (24) so that the liquid is layered on top of a first liquid, such as density gradient medium, which is present in the lower portion of the tube with minimal disruption or mixing as this will impair the separation process.
  • Figures 2a, 2b and 2c are top plan, front perspective and bottom plan views, respectively, of the insert (200) of Figure 1.
  • Figure 2a provides a top plan view of the insert (200), showing the arrangement of grooves or channels (128) running from the gripping element (140) in the centre of the disc (120) to the indentations (124) on the outer edge (122) or rim of the disc (120).
  • the gripping element (140) and prop (130) of the insert (200) are shown in the front perspective view of Figure 2b.
  • the underside plan view Figure 2c of the insert (200) shows the concave lower surface (126) of the disc (120) with central prop (130).
  • FIG. 3a A schematic top plan view of a disc (220) of an embodiment of an insert (300) according to the invention is illustrated in Figure 3a.
  • the disc (220) has fan shaped grooves extending radially from its centre (229) of its upper surface (221) to indentations (224) in the outer edge (222), the grooves (228) being narrower at the centre of the disc than at the outer edge.
  • the diameter (D) of the disc (220) will vary depending on the size or volume of the centrifuge tube for which the insert is intended; for example, a typical insert for a 50 ml centrifuge tube would have a disc of approximate diameter 26mm.
  • the curved indentations would be approximately 4mm in width (W) and have a depth (D) of approximately 1mm ( Figure 3b).
  • Figures 4a, b and c show schematic views of a portion of a disc of an embodiment of the invention.
  • Figure 4a depicts the flow of a liquid (as illustrated by the direction of flow of the arrows) over the upper surface (321) of the disc.
  • Liquid poured onto the top of the convex upper surface of the disc runs into the fan shaped grooves (328), flows down their length into the indentations (324) and then from the surface of the disc.
  • the depth of the grooves (328) increases from the centre of the disc where it meets with the gripping element (330) to a maximum at its outer edge (322).
  • Figure 4b is a schematic detail showing the height of the rise (H) of the non-inundated rim on the outer edge (322) of the disc, which for an insert for a 50 ml centrifuge tube would be approximately 1 mm.
  • Figure 4c shows the depth (D) of the groove (328), which would be approximately 1 mm for the insert shown. The depth D and height H would vary depending upon the volume of the tube for which the insert was designed.
  • Figure 5a, b and c are schematic representations of an insert in accordance with the invention positioned within an empty centrifuge tube (Figure 5a), a filled centrifuge tube prior to ( Figure 5b) and following centrifugation (Figure 5c).
  • FIG 5a shows an insert (500) according to the invention positioned within a conventional centrifuge tube (450), the tube having a lid (451) enclosing a hollowed interior (452) or chamber defined by its interior walls (453).
  • the centrifuge tube (450) of Figure 5a does not contain any liquid.
  • Both the exterior (453) and interior (454) walls of the tube are made from an inert material, such as plastic, glass or metal.
  • the centrifuge tube (450) may be designed to accommodate a range of liquid volumes, such as 5ml, 10ml, 15, and 50ml, the walls being marked (455) to indicate the volume present within the chamber (452).
  • the insert (500) comprises a disc (420), with gripping element (440) to allow positioning within the tube (450) and prop (430) to support the disc above the base of the tube.
  • the disc is sized such that the outer edge (422) is in contact with the interior walls (453) of the tube (450) to present a liquid impermeable barrier, such that liquid cannot pass from the upper portion of the tube to the lower portion of the tube except through indentations (424).
  • the indentations (424), present in the outer edge of the disc are designed to restrict flow of liquid across the disc in the absence of centrifugal force.
  • Figure 5b illustrates the tube (450) of Figure 5a filled with two liquids (460 and 462), a density gradient medium (460) and a liquid sample (462) containing cells, prior to centrifugation and separation of the cells from the liquid sample (462) into the density gradient medium.
  • a pre-defined volume of density gradient medium (460) is poured into the lower portion (A) of the tube and insert (500) subsequently inserted such that the meniscus of liquid (460) is in fluid connection with the lower surface (426) of disc (420) of the insert when it is supported above the base of the tube by prop (430).
  • the length of the prop (430) thus acts as a spacer to define a specific volume of liquid (460) or density gradient medium which can be present in the lower portion (A) of the centrifuge tube. It will be understood that the length of the prop (430) will vary depending upon the desired volume of liquid or density gradient medium (460) to be used in the centrifugation and separation process, and the internal volume of the centrifugation tube. For a typical 50 ml centrifuge tube, the length of the prop (430) is sized such that the disc (420) sits at the 15 ml mark of the tube (450).
  • the cap (451) is then replaced and the tube is transferred to a centrifuge.
  • Figure 5c is a schematic representation of the tube of Figure 5b following centrifugation.
  • the liquid present in the tube (450) has separated into four fractions, D, E, F and G based upon density.
  • Fraction D primarily contains plasma
  • fraction E is Ficoll
  • fraction F consists predominantly of red blood cells
  • fraction G contains white blood cells.
  • the target populations of cells can now be collected by carefully pipetting the various fractions from the tube: for example, if white blood cells are required then fraction D is first removed and then fraction G collected. Alternatively, if red blood cells are targeted, fractions D and G are discarded, the insert (500) carefully removed to avoid disturbing and remaining fractions, and fraction F collected.
  • An insert according to the invention was tested for its ability to assist with the loading of blood onto a Ficoll-Paque density gradient medium without mixing at the Blood-Ficoll interface and for its ability to allow blood fractions to separate without mixing under centrifugal force.
  • AccuspinTM tubes are specially designed polypropylene centrifuge tubes with two chambers separated by a porous high density polytheylene barrier or frit to aid the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow during centrifugation.
  • 25mls of the blood/PBS mix (562a, 562b) was carefully pipetted onto the surface of the Ficoll-Paque layer of both tube 550a and b.
  • An insert (600) according to the invention was added to the third tube (550c).
  • 25ml of the blood/PBS mix (562c) was added by pipetting to the top centre of the insert in tube 550c to allow the blood mix to flow down the grooves and form a layer above the Ficoll-Paque layer.
  • Figure 6 is a photograph of the three tubes (550a, 550b and 550c) which have been loaded with a Ficoll-Paque density gradient medium (lower layer, 560a, b and c) and the blood/PBS mix (upper layer, 562 a, b and c) prior to separation by centrifugation.
  • a Ficoll-Paque density gradient medium lower layer, 560a, b and c
  • the blood/PBS mix upper layer, 562 a, b and c
  • Figure 7 shows the same three tubes of Figure 6 following centrifugation and the separation process.
  • the red blood cells have pelleted at the bottom (664a, b and c) of the Ficoll-Paque layer (665a, b and c) of the tubes (650a, b and c) while the white blood cells (666a, b and c) have separated at the interface of the Ficoll-Paque (665a, b and c) and the plasma 667 (a, b and c) layers.
  • the white blood cell fractions from each tube were then harvested with a pipette and counted using a nucleocounter (NCI 00, Sartorius). 10 6 cells were analysed by flow cytometry (FACS Calibur, BD Bioscience) to determine their population composition.
  • Figure 8 is a graphical representation of the results from the flow cytometry analysis of the white blood cell fraction from each of the tubes (A corresponds to 650a, B to 650b and C to 650c) of Figure 7, the letters G indicating the Granulocyte sub-population, M the Monocyte sub-population and L the Lymphocyte sub-population present in the white blood cell fractions. The figures also show the percentage of each sub-population (Granulocytes, Monocytes and Lymphocytes) present in the white blood-cell population. Table 1 below gives the white blood cell recovery and cell viability for each of the tubes.
  • Tube containing No Insert 2.24 x 10 8 98.9% (e.g. Tube 650a)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
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  • Ecology (AREA)
  • Immunology (AREA)
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  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Cette invention concerne des dispositifs, des procédés et des kits pour séparer des matières biologiques, en particulier des populations de cellules de densités différentes. L'invention trouve une utilité particulière dans la séparation du sang en ses différents composants constitutifs ou populations cellulaires. Un insert destiné à un tube de centrifugation qui facilite la séparation efficace des fractions sanguines en obtenant une interface nette et distincte entre le milieu de gradient de densité et l'échantillon de sang est décrit. L'invention concerne en outre des procédés et des kits impliquant l'utilisation de l'insert pour séparer des cellules.
PCT/EP2015/073848 2014-10-16 2015-10-15 Dispositif et procédé de séparation de cellules WO2016059141A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP15780879.1A EP3206793A1 (fr) 2014-10-16 2015-10-15 Dispositif et procédé de séparation de cellules
US15/514,381 US10737262B2 (en) 2014-10-16 2015-10-15 Device and method for separating cells
JP2017518452A JP6652967B2 (ja) 2014-10-16 2015-10-15 細胞を分離するためのデバイス及び方法
CN201580055640.5A CN106796216B (zh) 2014-10-16 2015-10-15 用于分离细胞的装置和方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1418382.6A GB201418382D0 (en) 2014-10-16 2014-10-16 Device and method for separating cells
GB1418382.6 2014-10-16

Publications (1)

Publication Number Publication Date
WO2016059141A1 true WO2016059141A1 (fr) 2016-04-21

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US (1) US10737262B2 (fr)
EP (1) EP3206793A1 (fr)
JP (1) JP6652967B2 (fr)
CN (1) CN106796216B (fr)
GB (1) GB201418382D0 (fr)
WO (1) WO2016059141A1 (fr)

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JPWO2023008457A1 (fr) * 2021-07-28 2023-02-02

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US10737262B2 (en) 2020-08-11
EP3206793A1 (fr) 2017-08-23
GB201418382D0 (en) 2014-12-03
JP2017532196A (ja) 2017-11-02
US20170297016A1 (en) 2017-10-19
JP6652967B2 (ja) 2020-02-26
CN106796216A (zh) 2017-05-31

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