US3741400A - Blood sample container - Google Patents

Blood sample container Download PDF

Info

Publication number
US3741400A
US3741400A US3741400DA US3741400A US 3741400 A US3741400 A US 3741400A US 3741400D A US3741400D A US 3741400DA US 3741400 A US3741400 A US 3741400A
Authority
US
Grant status
Grant
Patent type
Prior art keywords
container
means
centrifuging
clip
test tube
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
Inventor
J Dick
Original Assignee
J Dick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Grant date

Links

Images

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0633Valves, specific forms thereof with moving parts

Abstract

A generally tubular shaped container includes an expandable head valve member and retaining clip assembly therewithin. On centrifuging the clip is disengaged from the valving member, permitting it to expand toward the inner walls of the container to form two separate compartments, one for the blood cells and one for the serum. During centrifuging, the valve member head is temporarily deformed at its edges an amount sufficient to allow the cells to move therepast, and at the conclusion of centrifuging the head resumes sealing engagement with the container wall. The serum may then be simply poured out of the container for testing. In a further aspect, the tubular container has a spring-loaded valving member disposed at substantially the mid-point thereof. In a still further form, filter means are located within a test tube, through which serum can pass, but blood cells cannot.

Description

O Umted States Patent 1191 1 1 ,4 Dick June 26, 1973 BLOOD SAMPLE CONTAINER Primar Examiner-Robert L. Lindsay, Jr.

J Inventor: J M. Dick, Burlingame Atl0rneyGe0rge J. Nettei' and Kendrick and SUbkOW Avenue, Los Angeles, Calif. 90620 [22] Filed: June 15, 1970 [57] ABSTRACT PP .1 6,168 A generally tubular shaped container includes an expandable head valve member and retaining clip assem- 521 US. c1 210/516, 210/010. 23 433/45 bly therewithincentrifuging the clip is disengaged 511 1111.01 B010 21/26 fmm valving Permitting it expand [58 Field of Search ..210/516-518, Wafd the Walls the 3e1m- 2 0/36 380, I30 DIG. 233/45 rate compartments, one for the blood cells and one for the serum. During centrifuging, the valve member head [56] References Cited is temporarily deformed at its edges an amount sufficient to allow the cells to move therepast, and at the UNITED STATES PATENTS conclusion of centrifuging the head resumes sealing enz gagement with the container wall. The serum may then aw e a 2,995,253 8/1961 Belgarde et al. 210/130 x be slmply poured out of the container for f 3,300,051 1/1967 Mitchell 210 361 x In a further p thesctubular contamer has 8 3,508,653 4/1970 Coleman 210/516 X spring-loaded valving member disposed at substantially 2,3 8 /19 Wheeler t t t 2l /5l6 the mid-point thereof, in a still further form, filter 3,583,230 6/1971 Patterson 2l0/3 6l X ans are located within a test tube, through which serum can pass, but blood cells cannot.

6 Claims, 7 Drawing Figures ,-Z5 3 }Z6 Z0 BLOOD SAMPLE CONTAINER BACKGROUND OF THE INVENTION The present invention pertains generally to containers for blood samples, and, more particularly, to such containers having separate compartments into which serum and blood cells are respectively received during centrifuging.

The frequent practice is for blood samples of a patient to be taken at a doctors office and then transmitted to a laboratory facility where analytical tests are conducted. Prior to transmitting the blood samples to the laboratory, it has been found advantageous to centrifuge the sample for separating the cells from the serum and thereby obtaining serum for test substantially free from cells. In the past, centrifuging has been accomplished by placing a blood sample in a conventional test tube, inserting the tube and sample in the centrifuge, and, after separating the cells from the blood serum, the serum (which is at the upper part) is then poured off, leaving the cells. This essentially hand technique involves a relatively large amount of time on the part of the doctor and/or nurse in the office, which could more profitably, and with benefit to the patient, be spent on other matters.

OBJECTS AND SUMMARY OF THE INVENTION There is'provided in accordance with the practice of the present invention, a blood sample container which on centrifuging automatically separates the blood and serum into separate compartments.

Another object of the invention is the provision of a blood sample container which, after centrifuging to separate the cells from the serum, can be transmitted as is, without further special handling or processing.

In the practice of the present invention there is provided a generally tubular shaped container for the blood sample including an expandable head valve member and retaining clip assembly therewithin into which the whole blood is initially inserted. On centrifuging the clip is disengaged from the valving member, permitting it to expand toward the inner walls of the container to form two separate compartments, a lower one primarily for blood cells and an upper one containing only serum. During centrifuging, the valve member head is temporarily deformed at its edges an amount sufficient to allow the cells to move therepast, and at the conclusion of centrifuging the head resumes sealing engagement with the container wall. The serum'may then be simply poured out of the container for testing.

A further form of the invention includes a tubular container with spring-loaded valving member disposed at substantially the mid-point thereof which is held initially open by means of a detachable clip. On centrifuging, the valving member is held open under the action of centrifugal force to permit the cells tomove to the container bottom, and at the close of centrifuging the valving member closes, separating the serum in the upper compartment from the cells in the lower one. The clip is detached during centrifuging. As in the first form, the serum may be poured out of the container while the cells are retained in the lower compartment.

A still further form of the invention includes a typical test tube with a filter means located midway, through which serum can pass, but blood cells cannot. n centrifuging, the serum is filtered and the cells remain above the filter as residue. At the conclusion of centri fuging, the filter means and residue are removed and discarded, leaving the serum in the tube for testing.

DESCRIPTION OF THE DRAWINGS FIG. 1 is a sectional, elevational view of a centrifuge including blood sample containers of the present invention.

FIG. 2 depicts in perspective view a preferred form of the invention in disassembled form.

FIG. 3 illustrates a sectional, elevational view of the invention as shown in FIG. 2 in assembled form prior to centrifuging.

FIG. 4 is a sectional, elevational view of the blood sample container of FIG. 3, shown immediately after initiation of centrifuging.

FIG. 5 is a sectional, elevational view of a further form of the invention.

FIG. 6 illustrates a retaining clip for use with the invention of FIG. 5.

FIG. 7 depicts a still further form of the invention.

DESCRIPTION OF A PREFERRED EMBODIMENT It is basic to the subject invention that a container for carrying a sample of blood in accordance with the practice of this invention is to be centrifuged and the serum and blood cells separated thereby with the cells confined to one portion of the container while the serum is confined in another. Also, it is an important advantage of this invention that the container after centrifuging may be readily shipped or mailed to a testing laboratory as is without further special handling or processing by the doctor.

FIG. 1 illustrates a centrifuge 10 of conventional design including a vertically extending axle ll driven by a rotative power source (not shown), located in a support base 12. The upper end of the shaft 11 is secured to two or more radially extending arms 13, the outer ends of which carry suitable receptacles 14 for the blood sample containers 15. An open top bowl-like member 16 conventionally surrounds the entire shaft, arms 13 and blood sample containers 15 as shown. In operation, the power source in the base 12 rotates the shaft and associated apparatus at a high rate of speed which tends to move the more dense blood cells within the blood sample toward the bottom of the blood sample container due to the action of centrifugal force.

Most small-sized centrifuges are designed for use with sample containers in the general shape of a test tube and for that reason the general outward characteristics of the container of this invention will have that same shape. It is to be understood, however, that other external container geometries may be adopted and still be within the spirit of this invention.

Reference is now-made to FIG. 2 and to the detailed construction of a preferred embodiment of the invention in which the main container body consists of a typical glass test tube 15 which may be closed by a conventional rubber or cork stopper 17. A centrifuging valve means identified generally as 18 in exploded form, includes two main parts, a releasable clamp 19 and a gravity actuated valving member 20.

More particularly, the valving member 20 includes a frusto-conical head 21 of circular periphery, which is received on the end of a rod 22 of such length that when the valving member assembly is fully received within the test tube, the head 21 is located at substantively. e r V r The clamp 19 includes aplurality-ofarrns joinedtogether at their ends to form a triangular base enclosing I conical head 21 as shown in FIG. 3 generally. at 26.

I tiall y the midpoint of the test tube long dimension. The

head 21 is constructed of a resilient plastic or rubber which is more dense than the whole blood, blood cells.

i i or serum thereofwhichenables the head to undergo a temporary deformation during centrifugingto produce.

a valving action that willbe described below. The, head diameter is such that in a normal rest condition within" the tube 15, as shown ins olid line depictioninFlGS. l 3 and 4; the head edges sealingly contact the innerwall of the tube in a continuous manner and separate the.

tube into upper and lowerchambers 23 and 24, respeca space through which the rod 22'is received. A plurality of upstanding hooks 25, attached to the base. of the;

clamp 19 extend in the same direction away from the base and are so dimensioned that they can be received about and engage the peripheral margins of the fru sto Prior to insertion of theqvalve means 18 within the like ear 37 is formedfrom the clip body, and a second hooklike ear 38 faceson thesame side of the clip as the first ear; The ear38 is at the end of a leverlarm 39 formed fromithe clip body and bent such that inthe relaxed state the ear 38.is retracted below theclip body surface as shown in FIG. 6. i I i i In. use the clip 36 mounted onto the spring 34 by 1 1 forcing the ear 38throug'h the openingin the clip body wall and with both ears then extending onthesame side f of the clip the earsrretaininglyengage thespringloops holding thespring partially coiled as illustratedin FIG.

5. The entire valve assembly 27 is then inserted into the tube 15, with the valvej initially held in the opencondi: v tionby the clip sane whole blood sample fills the lower chamber 29 and substantially all of chamber 28.

On centrifuging otthe sample, the ball closure mernber 35 further. compresses the spring 34 disengaging tube 15, the clamp 19 is engaged with th'e'head 2 1,

which moves the head margins inwardly a sufficient amount so thattheentire assembly means may be readily inserted into the test tube 15. That is, the clamp the test tube, whereas, as was indicated above, the at rest, unclamped diameter of the head 21 is slightly larger than the test tube cavity in order to permit sealing engagement with the test tube inner wall.

In use, the assembly 18with the clamp 19clonstrict- 3 ing the head 21 is'inserted into the tube and the blood sample to be centrifuged isplaced in the tube l filling A still further embodiment is that shown in 7 i lateral dimensions are less than the inner diameter of 1 theretaining clip which falls to the side. At the conclusion of centrifugingthe spring, which is now freeof the retaining Clip, seats the ball 35 within the opening seal- .ing the lower chamber 29 lcontaining blood'cells from ,the upper chamber 28 containing only serum. i

which relies on a filter for maintaining separation of the cells and serum. An annular member 46 has an outer diameter closely approximating theinnerldiarneter of the test tube'15. A porous filter element 40 is received within thev central opening of 39 andseats against a shoulder 4l, thereof. The filter elementisso'constructed astopermit serum to pass through while 're-r stricting the passageof blood cells. A plurality of elon chamber 23 and substantially all of chamber 24. The in tube is then placed in the centrifuge. During centrifuging the clamp 19 moves from a position of headengage ment shown in FIG. 3 to alower position asshown in FIG. 4, due to the'forceexerted thereon. Also, at this i time the" centrifugalforce deforms the margins ofthe resilient head inwardly to the dotted line position in. FIG. 4,=which permits the more dense blood cells to move to the lower chamber 24 'of the tube. The latter process continues until substantially all the cells have been centrifugedto the bottom chamber of the tube, at which time the centrifuge is'stopped. In the rest position the marginaledges of the head 21 reassume sealing contact with the inner walls of the tube 15 and the entire blood sample container may be simply removed from the centrifuge as is and sent to a laboratory by messenger or mail, asdesired, without handling or processing.

FIG. 5 depicts an alternate form of thisinvention including'a spring operated valve means27 for'separating the test tube 15 into an upper compartment 28 for serum and a lower one 29 for blood cells. The valve means comprises an annularvalve member 30 with a tapered opening 31, which member closely fitswithin further special umized and the blood sampleis emptieddirectly into g the test tube bore. A plurality of rodlike supports 32 in- 1 v terconnect the valve member 30 anda disclike pedestal 33. Within the space defined by the supports 32 a coil spring 34 has one end engaging the pedestal upper surface and its end contacting a ball closure member 35 of such diameter thatwhen received within the opening 31 it closes the same. When the spring is uninhibited, it seats the ball 35 within the opening 31.

A generally flat, elongated retaining clip 36 isconstructed of a springlike metal or plastic. A first hook- I gatedsupports 42 are affixedto the lower surface of the 7 member 39 and extend downwardly therefrom to terminate ata common junctionor pedestal 43. A retract;

ing means 44 is secured to the upper annular surface and terminatesin an eyelet 45.

ended device (not shownfinserted into'eyelet 45,1114:

entire filtering mechanismjis removed During such removaL due tothe close fit of the tube and annular" member 39, the cells are also'removed and the tube inner walls wiped clean,.leavin g the serum for testing.

Although not intendedto be limitative on the present invention, inthe usual case the containers ISare vacu the container from tlie'hypodermic via itsneedle. This is especially advantageous in the use of thefilter device of FIG. 7, since the low pressure below the filter element will tend to draw: the serum through the filter,

thereby reducing; if not eliminating the centrifuging; I

Iclaimr I Container apparatus actuatable by centrifuging force to segregate materials of'differing density com- I posing a liquid sample carried thereby, comprising in combination: a test tube; a support rod; a pliable sealing member received onto one end of the support rod, saidsealing member having transv v 6 verse dimensions greater than the test tube inner in a compressed state. diameter; and 4. A fluid sample container adapted for receipt a clip received onto the support rod and including within a centrifuge, comprising in combination:

clasps for engaging the sealing member margins test tube container means having an open end and a from the rod side and constricting them inwardly; 5 closed end; and said rod, sealing member and clip being received force actuated valve means carried within said conwithin the test tube with the rod engaging the tube tainer means, said valve means including, bottom whereby during centrifuging the clip is a resiliently deformable head element of dimenforced off the sealing member and the sealing sions slightly exceeding those of the container member edges are bent toward the tube bottom al- 10 means transverse internal cavity received within lowing denser materials of the liquid sample to said container means, and pass. I support means connected to said head element and 2. Container apparatus actuatable during centrifugresting on the inner closed end of said container ing for separating blood cells from serum composing a means for holding the head element spaced from blood sample carried thereby, comprising in combinasaid container means closed end, the peripheral tion: margins of said head element deforming during a test tube; centrifuging to allow suspended materials in the a disc valve seat fittingly received within the test tube fluid sample to pass into the container means and including an opening passing therethrough; cavity between said head element and said consupport members connected to the valve seat and extainer meand closed end.-

tending downwardly in the tube; 5. A fluid sample container as in claim 5, in which the pedestal means secured to the lower ends of said suphead element is of frusto-conical shape, the convex port members; part thereof facing toward the container means open a coil spring having one end engaging the pedestal end.

means and extending upwardly toward the valve 6. A fluid sample container as in claim 5, in which a seat; and retaining clip is clampingly received onto the head elea closure member actuated by the free end of the ment deforming the element edge margins sufficiently spring to close the opening in the valve seat when to allow the fluid sample to pass around said margins the spring is in a relaxed state. into the lower reaches of said container means, said 3. Container apparatus as in claim 2, in which there clip being disengaged from said head element by the is further provided clip means for engaging selected centrifuging force. 1 loops of said spring for maintaining the spring initially

Claims (6)

1. Container apparatus actuatable by centrifuging force to segregate materials of differing density composing a liquid sample carried thereby, comprising in combination: a test tube; a support rod; a pliable sealing member received onto one end of the support rod, said sealing member having transverse dimensions greater than the test tube inner diameter; and a clip received onto the support rod and including clasps for engaging the sealing member margins from the rod side and constricting them inwardly; said rod, sealing member and clip being received within the test tube with the rod engaging the tube bottom whereby during centrifuging the clip is forced off the sealing member and the sealing member edges are bent toward the tube bottom allowing denser materials of the liquid sample to pass.
2. Container apparatus actuatable during centrifuging for separating blood cells from serum composing a blood sample carried thereby, comprising in combination: a test tube; a disc valve seat fittingly received within the test tube and including an opening passing therethrough; support members connected to the valve seat and extending downwardly in the tube; pedestal means secured to the lower ends of said support memberS; a coil spring having one end engaging the pedestal means and extending upwardly toward the valve seat; and a closure member actuated by the free end of the spring to close the opening in the valve seat when the spring is in a relaxed state.
3. Container apparatus as in claim 2, in which there is further provided clip means for engaging selected loops of said spring for maintaining the spring initially in a compressed state.
4. A fluid sample container adapted for receipt within a centrifuge, comprising in combination: test tube container means having an open end and a closed end; and force actuated valve means carried within said container means, said valve means including, a resiliently deformable head element of dimensions slightly exceeding those of the container means transverse internal cavity received within said container means, and support means connected to said head element and resting on the inner closed end of said container means for holding the head element spaced from said container means closed end, the peripheral margins of said head element deforming during centrifuging to allow suspended materials in the fluid sample to pass into the container means cavity between said head element and said container meand closed end.
5. A fluid sample container as in claim 5, in which the head element is of frusto-conical shape, the convex part thereof facing toward the container means open end.
6. A fluid sample container as in claim 5, in which a retaining clip is clampingly received onto the head element deforming the element edge margins sufficiently to allow the fluid sample to pass around said margins into the lower reaches of said container means, said clip being disengaged from said head element by the centrifuging force.
US3741400A 1970-06-15 1970-06-15 Blood sample container Expired - Lifetime US3741400A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US4616870 true 1970-06-15 1970-06-15

Publications (1)

Publication Number Publication Date
US3741400A true US3741400A (en) 1973-06-26

Family

ID=21941980

Family Applications (1)

Application Number Title Priority Date Filing Date
US3741400A Expired - Lifetime US3741400A (en) 1970-06-15 1970-06-15 Blood sample container

Country Status (2)

Country Link
US (1) US3741400A (en)
CA (1) CA936847A (en)

Cited By (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3897340A (en) * 1974-02-27 1975-07-29 Becton Dickinson Co Serum/plasma separator assembly with interface-seeking piston having coarse and fine band filters
US3897337A (en) * 1974-02-27 1975-07-29 Becton Dickinson Co Plasma separator assembly having interface-seeking piston with centrifugal valve
US3935113A (en) * 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US3945928A (en) * 1974-02-27 1976-03-23 Becton, Dickinson And Company Serum/plasma separators with centrifugal valves
US3951801A (en) * 1974-02-27 1976-04-20 Becton, Dickinson And Company Serum/plasma separator-strut stop type
US3954624A (en) * 1974-04-17 1976-05-04 Amf Incorporated Filter cartridge assembly
FR2350274A1 (en) * 1976-05-06 1977-12-02 Sherwood Medical Ind Inc Device intended manifold to receive a fluid sample to be separated into heavy and light phases
US4189385A (en) * 1977-05-03 1980-02-19 Greenspan Donald J Method and apparatus for separating serum or plasma from the formed elements of the blood
EP0198462A2 (en) * 1985-04-18 1986-10-22 Opopharma A.G. Separation of materials from a liquid dispersion by sedimentation
US4828716A (en) * 1987-04-03 1989-05-09 Andronic Devices, Ltd. Apparatus and method for separating phases of blood
US5019243A (en) * 1987-04-03 1991-05-28 Mcewen James A Apparatus for collecting blood
US5271852A (en) * 1992-05-01 1993-12-21 E. I. Du Pont De Nemours And Company Centrifugal methods using a phase-separation tube
US5282981A (en) * 1992-05-01 1994-02-01 E. I. Du Pont De Nemours And Company Flow restrictor-separation device
US5354483A (en) * 1992-10-01 1994-10-11 Andronic Technologies, Inc. Double-ended tube for separating phases of blood
WO1996006679A1 (en) * 1994-08-31 1996-03-07 Activated Cell Therapy, Inc. Centrifuge syringe apparatus and method
WO2000046585A2 (en) 1999-02-03 2000-08-10 Dahm Michael W Method for enriching or depleting tumour cells obtained from a body fluid and kit suitable for this purpose
US6582904B2 (en) 1995-11-16 2003-06-24 Michael W. Dahm Method of quantifying tumour cells in a body fluid and a suitable test kit
US6821726B1 (en) 1998-02-04 2004-11-23 Michael W. Dahm Method for quantitatively analyzing tumor cells in a body fluid and test kits suited therefor
US20070036686A1 (en) * 2005-05-31 2007-02-15 Mehdi Hatamian Systems for tracking and testing of medical specimens and data
US20100140182A1 (en) * 2008-12-04 2010-06-10 Chapman John R Apparatus and method for separating and isolating components of a biological fluid
US20100160135A1 (en) * 2008-07-21 2010-06-24 Becton, Dickinson And Company Density Phase Separation Device
US20100288694A1 (en) * 2009-05-15 2010-11-18 Becton, Dickinson And Company Density Phase Separation Device
US20110192804A1 (en) * 2006-05-25 2011-08-11 Biomet Biologics, Llc Apparatus and Method for Separating and Concentrating Fluids Containing Multiple Components
WO2012083228A1 (en) * 2010-12-17 2012-06-21 Emil Kakkis Biomarkers for assessing sialic acid deficiencies
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
US8337711B2 (en) 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
US8394342B2 (en) 2008-07-21 2013-03-12 Becton, Dickinson And Company Density phase separation device
US20130241099A1 (en) * 1999-12-03 2013-09-19 Becton, Dickinson And Company Device for Separating Components of a Fluid Sample
WO2014022653A1 (en) * 2012-08-02 2014-02-06 Siemens Healthcare Diagnostics Inc. Biological liquid collection vessels, systems, and methods
US8783470B2 (en) 2009-03-06 2014-07-22 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8950586B2 (en) 2002-05-03 2015-02-10 Hanuman Llc Methods and apparatus for isolating platelets from blood
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US9114334B2 (en) 2002-05-24 2015-08-25 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9138664B2 (en) 2007-04-12 2015-09-22 Biomet Biologics, Llc Buoy fractionation system
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US9533090B2 (en) 2010-04-12 2017-01-03 Biomet Biologics, Llc Method and apparatus for separating a material
US9556243B2 (en) 2013-03-15 2017-01-31 Biomet Biologies, LLC Methods for making cytokine compositions from tissues using non-centrifugal methods
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9649579B2 (en) 2007-04-12 2017-05-16 Hanuman Llc Buoy suspension fractionation system
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
US9701728B2 (en) 2008-02-27 2017-07-11 Biomet Biologics, Llc Methods and compositions for delivering interleukin-1 receptor antagonist
US9897589B2 (en) 2002-05-24 2018-02-20 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US982328A (en) * 1910-10-31 1911-01-24 Henry H Wheeler Liquid-separating device.
US1528480A (en) * 1921-04-16 1925-03-03 Cream Top Bottle Corp Milk bottle and cream separator for use therewith
US2963162A (en) * 1959-07-22 1960-12-06 Cummins Engine Co Inc Oil filter spool construction
US2995253A (en) * 1959-12-10 1961-08-08 Fram Corp Combined full-flow and part-flow oil filter
US3300051A (en) * 1963-09-26 1967-01-24 Internat Equipment Co Filter tube for use in a centrifuge
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
US3583230A (en) * 1968-06-12 1971-06-08 Sondell Research Dev Co Sample injection method and apparatus

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US982328A (en) * 1910-10-31 1911-01-24 Henry H Wheeler Liquid-separating device.
US1528480A (en) * 1921-04-16 1925-03-03 Cream Top Bottle Corp Milk bottle and cream separator for use therewith
US2963162A (en) * 1959-07-22 1960-12-06 Cummins Engine Co Inc Oil filter spool construction
US2995253A (en) * 1959-12-10 1961-08-08 Fram Corp Combined full-flow and part-flow oil filter
US3300051A (en) * 1963-09-26 1967-01-24 Internat Equipment Co Filter tube for use in a centrifuge
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
US3583230A (en) * 1968-06-12 1971-06-08 Sondell Research Dev Co Sample injection method and apparatus

Cited By (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3897337A (en) * 1974-02-27 1975-07-29 Becton Dickinson Co Plasma separator assembly having interface-seeking piston with centrifugal valve
US3935113A (en) * 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US3945928A (en) * 1974-02-27 1976-03-23 Becton, Dickinson And Company Serum/plasma separators with centrifugal valves
US3951801A (en) * 1974-02-27 1976-04-20 Becton, Dickinson And Company Serum/plasma separator-strut stop type
US3897340A (en) * 1974-02-27 1975-07-29 Becton Dickinson Co Serum/plasma separator assembly with interface-seeking piston having coarse and fine band filters
US3954624A (en) * 1974-04-17 1976-05-04 Amf Incorporated Filter cartridge assembly
FR2350274A1 (en) * 1976-05-06 1977-12-02 Sherwood Medical Ind Inc Device intended manifold to receive a fluid sample to be separated into heavy and light phases
US4152270A (en) * 1976-05-06 1979-05-01 Sherwood Medical Industries Inc. Phase separation device
US4189385A (en) * 1977-05-03 1980-02-19 Greenspan Donald J Method and apparatus for separating serum or plasma from the formed elements of the blood
EP0198462A3 (en) * 1985-04-18 1988-11-02 Assaf Pharmaceutical Industries Ltd. Separation of materials from a liquid dispersion by sedimentation
EP0198462A2 (en) * 1985-04-18 1986-10-22 Opopharma A.G. Separation of materials from a liquid dispersion by sedimentation
US4828716A (en) * 1987-04-03 1989-05-09 Andronic Devices, Ltd. Apparatus and method for separating phases of blood
US5019243A (en) * 1987-04-03 1991-05-28 Mcewen James A Apparatus for collecting blood
US5308506A (en) * 1987-04-03 1994-05-03 Mcewen James A Apparatus and method for separating a sample of blood
US5271852A (en) * 1992-05-01 1993-12-21 E. I. Du Pont De Nemours And Company Centrifugal methods using a phase-separation tube
US5282981A (en) * 1992-05-01 1994-02-01 E. I. Du Pont De Nemours And Company Flow restrictor-separation device
US5419835A (en) * 1992-05-01 1995-05-30 E. I. Du Pont De Nemours And Company Flow restrictor-separation device
US5354483A (en) * 1992-10-01 1994-10-11 Andronic Technologies, Inc. Double-ended tube for separating phases of blood
WO1996006679A1 (en) * 1994-08-31 1996-03-07 Activated Cell Therapy, Inc. Centrifuge syringe apparatus and method
US6582904B2 (en) 1995-11-16 2003-06-24 Michael W. Dahm Method of quantifying tumour cells in a body fluid and a suitable test kit
US6821726B1 (en) 1998-02-04 2004-11-23 Michael W. Dahm Method for quantitatively analyzing tumor cells in a body fluid and test kits suited therefor
WO2000046585A3 (en) * 1999-02-03 2001-04-26 Michael W Dahm Method for enriching or depleting tumour cells obtained from a body fluid and kit suitable for this purpose
US7211433B1 (en) 1999-02-03 2007-05-01 Hexal Gentech Forschungs Gmbh Method for the enriching or depleting tumor cells obtained from a body fluid and kit suitable for this purpose
WO2000046585A2 (en) 1999-02-03 2000-08-10 Dahm Michael W Method for enriching or depleting tumour cells obtained from a body fluid and kit suitable for this purpose
US9682373B2 (en) * 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US20130241099A1 (en) * 1999-12-03 2013-09-19 Becton, Dickinson And Company Device for Separating Components of a Fluid Sample
US8950586B2 (en) 2002-05-03 2015-02-10 Hanuman Llc Methods and apparatus for isolating platelets from blood
US9114334B2 (en) 2002-05-24 2015-08-25 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9897589B2 (en) 2002-05-24 2018-02-20 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US20070036686A1 (en) * 2005-05-31 2007-02-15 Mehdi Hatamian Systems for tracking and testing of medical specimens and data
US20110192804A1 (en) * 2006-05-25 2011-08-11 Biomet Biologics, Llc Apparatus and Method for Separating and Concentrating Fluids Containing Multiple Components
US8567609B2 (en) * 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9138664B2 (en) 2007-04-12 2015-09-22 Biomet Biologics, Llc Buoy fractionation system
US9649579B2 (en) 2007-04-12 2017-05-16 Hanuman Llc Buoy suspension fractionation system
US9701728B2 (en) 2008-02-27 2017-07-11 Biomet Biologics, Llc Methods and compositions for delivering interleukin-1 receptor antagonist
US8801586B2 (en) * 2008-02-29 2014-08-12 Biomet Biologics, Llc System and process for separating a material
US8337711B2 (en) 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
US20130196425A1 (en) * 2008-02-29 2013-08-01 Biomet Biologics, Llc System and Process for Separating a Material
US9719063B2 (en) 2008-02-29 2017-08-01 Biomet Biologics, Llc System and process for separating a material
US20100160135A1 (en) * 2008-07-21 2010-06-24 Becton, Dickinson And Company Density Phase Separation Device
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US8394342B2 (en) 2008-07-21 2013-03-12 Becton, Dickinson And Company Density phase separation device
US9714890B2 (en) 2008-07-21 2017-07-25 Becton, Dickinson And Company Density phase separation device
US8747781B2 (en) 2008-07-21 2014-06-10 Becton, Dickinson And Company Density phase separation device
US9933344B2 (en) 2008-07-21 2018-04-03 Becton, Dickinson And Company Density phase separation device
US9700886B2 (en) 2008-07-21 2017-07-11 Becton, Dickinson And Company Density phase separation device
US9452427B2 (en) 2008-07-21 2016-09-27 Becton, Dickinson And Company Density phase separation device
US9339741B2 (en) 2008-07-21 2016-05-17 Becton, Dickinson And Company Density phase separation device
US20120122649A1 (en) * 2008-12-04 2012-05-17 Chapman John R Apparatus and method for separating and isolating components of a biological fluid
US8177072B2 (en) 2008-12-04 2012-05-15 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US20120193274A1 (en) * 2008-12-04 2012-08-02 Chapman John R Apparatus and method for separating and isolating components of a biological fluid
US9375661B2 (en) 2008-12-04 2016-06-28 Cesca Therapeutics, Inc. Apparatus and method for separating and isolating components of a biological fluid
US20100140182A1 (en) * 2008-12-04 2010-06-10 Chapman John R Apparatus and method for separating and isolating components of a biological fluid
US8506823B2 (en) * 2008-12-04 2013-08-13 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US8511479B2 (en) * 2008-12-04 2013-08-20 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US8511480B2 (en) * 2008-12-04 2013-08-20 Thermogenesis Corp. Apparatus and method for separating and isolating components of a biological fluid
US8783470B2 (en) 2009-03-06 2014-07-22 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
US8992862B2 (en) 2009-04-03 2015-03-31 Biomet Biologics, Llc All-in-one means of separating blood components
US8998000B2 (en) 2009-05-15 2015-04-07 Becton, Dickinson And Company Density phase separation device
US20100288694A1 (en) * 2009-05-15 2010-11-18 Becton, Dickinson And Company Density Phase Separation Device
US9919308B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US9919309B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US9802189B2 (en) 2009-05-15 2017-10-31 Becton, Dickinson And Company Density phase separation device
US9079123B2 (en) 2009-05-15 2015-07-14 Becton, Dickinson And Company Density phase separation device
US9919307B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US8794452B2 (en) 2009-05-15 2014-08-05 Becton, Dickinson And Company Density phase separation device
US9364828B2 (en) 2009-05-15 2016-06-14 Becton, Dickinson And Company Density phase separation device
US9731290B2 (en) 2009-05-15 2017-08-15 Becton, Dickinson And Company Density phase separation device
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US9533090B2 (en) 2010-04-12 2017-01-03 Biomet Biologics, Llc Method and apparatus for separating a material
WO2012083228A1 (en) * 2010-12-17 2012-06-21 Emil Kakkis Biomarkers for assessing sialic acid deficiencies
US9239276B2 (en) 2011-04-19 2016-01-19 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
WO2014022653A1 (en) * 2012-08-02 2014-02-06 Siemens Healthcare Diagnostics Inc. Biological liquid collection vessels, systems, and methods
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US9556243B2 (en) 2013-03-15 2017-01-31 Biomet Biologies, LLC Methods for making cytokine compositions from tissues using non-centrifugal methods
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid

Also Published As

Publication number Publication date Type
CA936847A (en) 1973-11-13 grant
CA936847A1 (en) grant

Similar Documents

Publication Publication Date Title
US3244362A (en) Centrifuging apparatus and fractionating system
US3935113A (en) Serum/plasma separator with centrifugal valve
US5270219A (en) Fluid transfer device
US4425235A (en) Blood collection device with phase partitioning means
US5312758A (en) Method and device for combined enrichment, processing and embedding of cytological specimens according to histological principles
US5632895A (en) Serum separating device and apparatus for serum separation
US6516953B1 (en) Device for separating components of a fluid sample
US5257984A (en) Blood collector
US3941699A (en) Plasma separator with centrifugal valve
US5251474A (en) Centrifuged material layer measurement in an evacuated tube
US4040959A (en) Multi-purpose blood bag
US4720284A (en) Method and means for separation of blood components
US7470371B2 (en) Methods and apparatus for isolating platelets from blood
US4953561A (en) Urine testing module and method of collecting urine antigen
US5422018A (en) Centrifuge tube and adaptor
US4832842A (en) Filter chest for use with multiple filter units, for laboratories, and with the filter units
US3799342A (en) Method of using a serum separator
US4410630A (en) Lysis filtration culture chamber
US5955032A (en) Collection container assembly
US20010008614A1 (en) Sample collection system and method of use thereof
US4269718A (en) Process and device for centrifugal separation of platelets
US5560830A (en) Separator float and tubular body for blood collection and separation and method of use thereof
US4811866A (en) Method and apparatus for dispensing liquids
US3045494A (en) Method of providing for blood count and pipette and assembly for use therein
US3786985A (en) Blood collection container