WO2015198866A1 - 培養容器 - Google Patents
培養容器 Download PDFInfo
- Publication number
- WO2015198866A1 WO2015198866A1 PCT/JP2015/066767 JP2015066767W WO2015198866A1 WO 2015198866 A1 WO2015198866 A1 WO 2015198866A1 JP 2015066767 W JP2015066767 W JP 2015066767W WO 2015198866 A1 WO2015198866 A1 WO 2015198866A1
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- WO
- WIPO (PCT)
- Prior art keywords
- pressing member
- cell culture
- container
- recess
- bubble
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0848—Specific forms of parts of containers
- B01L2300/0854—Double walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
Definitions
- the present invention relates to a culture vessel, particularly a culture vessel that contains a culture solution used for microscopic observation.
- the inner diameter of the concave portion that accommodates the culture solution in the container becomes, for example, 16.5 mm or less
- the phenomenon that the liquid level of the culture solution in the container bends due to surface tension causes a problem in microscopic observation. That is, when observing cells and fungi with an optical microscope, the image is degraded by the lens effect caused by the meniscus. Therefore, it becomes difficult to observe other than the central portion of the recess.
- a meniscus control plate is floated in the container.
- An object of the present invention is to enable accurate observation by removing a meniscus in a culture vessel.
- a culture container includes a container body and a pressing member.
- the container body has a recess for accommodating the culture medium.
- the pressing member is a member that is detachably fitted to the upper side of the recess.
- the pressing member has a lower surface that comes into contact with the upper surface of the culture solution in a fitted state, and a bubble discharge portion that allows the bubbles in the culture solution to be discharged to the outside.
- the pressing member is fitted on the upper side of the recess in a state where the culture solution is accommodated in the recess of the container body. Then, the lower surface of the pressing member comes into contact with the upper surface of the culture solution, thereby eliminating the meniscus. In this state, the bubbles in the culture solution are discharged to the outside from the bubble discharge portion of the pressing member. As a result, the cells can be accurately observed in the culture container.
- the lower surface of the pressing member may at least partially have an inclined surface for guiding the bubbles in the culture solution toward the bubble discharge portion.
- the bubbles in the culture solution are guided to the bubble discharge portion by the inclined surface on the lower surface of the pressing member. Therefore, bubbles are unlikely to stay in the vicinity of the lower surface of the pressing member.
- the lower surface of the pressing member may be at least partially hydrophilized. In this container, bubbles are unlikely to stay in the vicinity of the lower surface of the pressing member.
- the holding member may further be formed with a liquid supply unit that is used to move the bubbles in the culture solution to the bubble discharge unit side by injecting the liquid into the culture solution.
- a liquid supply unit that is used to move the bubbles in the culture solution to the bubble discharge unit side by injecting the liquid into the culture solution.
- the bottom of the recess may have a first portion in which a plurality of microwells are formed. In that case, the lower surface of the pressing member contacts the upper surface of the culture solution.
- the bottom of the recess may have a second portion in which a plurality of microwells are not formed.
- the bubble releasing portion of the pressing member is provided corresponding to the second portion. Since no bubble emitting part is formed at a position corresponding to the plurality of microwells at the bottom, spheroids grown in the plurality of microwells can be observed accurately.
- Sectional drawing of a cell culture container. The top view of a part of cell culture container (1st Embodiment).
- VI-VI sectional drawing of FIG. VII-VII sectional view of FIG. The top view of a part of cell culture container (2nd Embodiment).
- IX-IX sectional view of FIG. The top view of a part of cell culture container (3rd Embodiment).
- XI-XI sectional drawing of FIG. The top view of a part of cell culture container (4th Embodiment).
- the top view of a part of cell culture container (5th Embodiment).
- XV-XV sectional drawing of FIG. The top view of a part of cell culture container (6th Embodiment).
- XVII-XVII sectional drawing of FIG. The top view of a part of cell culture container (7th Embodiment).
- XIX-XIX sectional view of FIG. Sectional drawing of a cell culture container (8th Embodiment). Sectional drawing of a cell culture container (9th Embodiment). Sectional drawing of a cell culture container. Sectional drawing of a cell culture container. Sectional drawing of a cell culture container. Sectional drawing of a cell culture container.
- the top view of a pressing member A top view of a pressing member (10th embodiment).
- Sectional drawing of a cell culture container (11th Embodiment). Sectional drawing of the container main body of a cell culture container (12th Embodiment). Sectional drawing of a cell culture container (13th Embodiment). The perspective view of the container main body of a cell culture container (15th Embodiment).
- FIG. 1 is a perspective view of a container body of a cell culture container.
- 2 and 3 are perspective views of a plate with a pressing member of the cell culture container.
- the cell culture container 1 includes a container body 3 and a plate 5 with a pressing member.
- the container body 3 has a rectangular shape in plan view, and has a plurality of recesses (wells) 7 for accommodating a cell culture solution C as a culture solution.
- the container body 3 is a transparent member having a small thickness, and is made of, for example, a transparent plastic.
- the container main body 3 is a well-known thing, for example, is a common well plate.
- the plate 5 with a pressing member has a rectangular shape corresponding to the container main body 3 and has a plurality of pressing members 9 corresponding to the recesses 7.
- the plate 5 with a pressing member is a thin member having a small thickness, and is made of, for example, a transparent plastic.
- the plate 5 with a pressing member has a flat main body 5a and a frame 5b formed on the outer peripheral surface thereof.
- a plurality of pressing members 9 are provided on the main body 5a of the plate 5 with pressing members.
- the pressing member 9 is a member that is detachably fitted to the upper side of the recess 7. 2 includes a total of 24 pressing members 9 of 6 ⁇ 4, and the pressing plate 5A illustrated in FIG. 3 includes a total of six pressing members 9 of 6 ⁇ 1. is doing.
- boards 5A with a pressing member shown in FIG. 3 are used with respect to the container main body 3 shown in FIG.
- FIG. 4 is a cross-sectional view of the cell culture container.
- the plurality of pressing members 9 enter the plurality of concave portions 7, respectively, and the concave portions 7 containing the cell culture solution C are sealed. is doing.
- decrease by volatilization of the cell culture solution C is suppressed.
- the lid is covered from above the cell culture vessel 1 (that is, from above the plate 5 with a pressing member) during cell culture.
- the lid covers the upper portion of the container body 3 and the plate 5 with the pressing member. Thereby, the contamination with respect to a cell culture solution is prevented.
- Observation of cells in the cell culture vessel 1 is performed from below the concave portions 7 using various microscopes. Specifically, since the cells are often adhered to the bottom of the recess 7, the cells are observed in such a state. However, when floating culture is performed, cells floating in the cell culture medium C may be observed.
- FIG. 5 is a plan view of a part of the cell culture container. 6 is a sectional view taken along the line VI-VI in FIG. 5, and FIG. 7 is a sectional view taken along the line VII-VII in FIG.
- the pressing member 9 has a lower surface 23a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing part 13 that allows the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble discharge part 13 is a recessed part formed on the outer peripheral surface of the cylindrical part 25 and communicating the outer peripheral side of the lower surface 23 a (described later) and the outside of the pressing member 9.
- the concave portion 7 has a bottom portion 17 and a cylindrical portion 19.
- the inner peripheral surface 19a of the cylindrical portion 19 extends substantially vertically in the longitudinal section.
- the pressing member 9 mainly has a lower surface portion 23 and a cylindrical portion 25.
- the lower surface portion 23 is a circular flat plate in plan view.
- the tubular portion 25 is fitted in the tubular portion 19 of the recess 7 with a slight gap. That is, the outer peripheral surface 25 a of the cylindrical portion 25 is opposed to the inner peripheral surface 19 a of the cylindrical portion 19 in a state of being close to the inner peripheral surface 19 a.
- the outer peripheral edge of the lower surface portion 23 is connected to the lower end of the cylindrical portion 25.
- the pressing member 9 further has a flange portion 27.
- the flange portion 27 is described as a member fixed to the main body 5a of the plate 5 with a pressing member, but may be formed integrally with the main body 5a.
- the flange portion 27 extends from the tubular portion 25 to the outer peripheral side, and is seated on the upper surface of the tubular portion 19 of the recess 7.
- the pressing member 9 is provided with the flange portion 27, it is easy to remove the pressing member 9 from the recess 7.
- a bubble discharge portion 13 is formed in a part of the pressing member 9.
- the bubble releasing unit 13 has a structure that allows the bubbles in the cell culture medium C to be released to the outside.
- the bubble discharge part 13 is a recessed part formed by cutting out a part of the outer peripheral surface 25a of the cylindrical part 25, and this recessed part is a lower surface of the lower surface part 23 as shown in FIG. It extends from 23 a to the outside of the cell culture container 1.
- the flange portion 27 is cut out in an arc shape in plan view. Since the bubble discharge portion 13 is provided on the pressing member 9 as described above, the meniscus is eliminated by the pressing member 9 and the retention of bubbles is also prevented. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 23a of the lower surface part 23 has a planar shape, but is not horizontal but inclined (see angle ⁇ in FIG. 7). More specifically, the lower surface 23a is inclined so that the vicinity of the bubble emitting portion 13 is the highest. Thereby, the air bubbles in the vicinity of the lower surface 23a are guided toward the air bubble discharge portion 13 along the lower surface 23a.
- the inclination angle ⁇ of the lower surface 23a is 1 to 30 degrees, and more preferably 1 to 5 degrees.
- the lower surface 23a of the lower surface part 23 is subjected to a hydrophilic treatment. Therefore, bubbles are unlikely to stay in the vicinity of the lower surface 23a.
- a known technique for example, application of a hydrophilic polymer
- the hydrophilic treatment prevents cells cultured in suspension culture from adhering to the lower surface 23 a of the lower surface portion 23.
- a liquid supply part 31 is formed on the pressing member 9.
- the liquid supply unit 31 is used to move the bubbles in the cell culture solution C to the bubble discharge unit 13 side by injecting the liquid into the cell culture solution C.
- the liquid supply part 31 is a recess formed by cutting out a part of the outer peripheral surface 25a of the cylindrical part 25.
- the recess is formed from the lower surface 23a of the lower surface part 23 to the cell culture container 1. It extends to the outside.
- the liquid supply unit 31 is formed at a position facing the bubble discharge unit 13 in the radial direction. That is, the liquid supply unit 31 is disposed at the lowest position on the lower surface 23a.
- the bubbles in the cell culture solution C are moved to the bubble discharge unit 13 side. Therefore, bubbles are unlikely to stay in the vicinity of the lower surface 23 a of the pressing member 9.
- a pipette is used for the liquid supply.
- release part 13 and the liquid supply part 31 enable the insertion seeding
- the lower surface portion 23 of the pressing member 9 is in contact with the upper surface of the cell culture medium C, the meniscus problem is solved. Furthermore, since the outer peripheral edge of the lower surface portion 23 extends to the vicinity of the inner peripheral surface 19a of the concave portion 7, the observation area is increased. Conventionally, for example, the range that can be observed without being affected by the meniscus in a concave portion having a diameter of about 7 mm is about 1 to 3 mm, but in this embodiment, the thickness of the pressing member 9 is about 5 mm except for the wall thickness. It becomes observable in the range. As a result, it is possible to confirm the region that has been determined by inference in the past, and it is possible to accurately change with time and observe the state.
- the area of the opening portion of the pressing member 9 by the slit is 1 to 50%, preferably 3 to 30% with respect to the area of the lower surface portion 23.
- the operation of fitting the holding member 9 into the recess 7 will be briefly described.
- the cell culture medium C is injected into the recess 7.
- the amount of the cell culture solution C at this time is, for example, a position where the lower surface 23a of the pressing member 9 contacts the upper surface of the cell culture solution.
- the pressing member 9 is fitted into the recess 7.
- the liquid surface of the cell culture solution C is pressed by the lower surface 23 a. A part of the cell culture solution C enters the bubble discharge unit 13 and the liquid supply unit 31.
- the inclination of the lower surface 23a may be omitted or may be another shape.
- the lower surface 23a may be a two-stage inclined surface having a large inclination angle near the bubble emitting portion.
- the hydrophilization process of the lower surface 23a may be abbreviate
- the liquid supply part 31 may be omitted or may have another shape.
- the size of the pressing member and the recess in the embodiment will be described.
- the following specific examples illustrate the size of the well where the meniscus becomes remarkable, and the present invention is not limited to these numerical values.
- the inner diameter of the pressing member is 8.0 to 14.0 mm
- the vertical length of the pressing member (excluding the flange) is 3.0 to 10.0 mm
- the outer diameter of the pressing member is The inner diameter of the bottom surface of the recess is 14.0 to 17.0 mm.
- the inner diameter of the pressing member is 4.0 to 5.0 mm
- the vertical length of the pressing member (excluding the flange) is 3.0 to 10.0 mm
- the outer diameter of the pressing member is The inner diameter of the bottom surface of the recess is 5.0 to 7.0 mm.
- FIG. 8 is a plan view of a part of the cell culture container.
- 9 is a cross-sectional view taken along the line IX-IX in FIG.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the pressing member 9 has a lower surface 41a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing portion 43 that allows the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble discharge part 43 is a plurality of grooves extending in an arc shape and penetrating vertically in the outer peripheral edge of the lower surface part 41.
- the bubble discharge part 43 has a pair of first grooves 43a on the right side of the drawing and a pair of second grooves 43b on the left side of the drawing.
- the pair of first grooves 43a on the right side of the drawing extends over the entire outer peripheral edge of the lower half 41 on the right side of the drawing.
- the pair of second grooves 43b on the left side of the drawing extends over the entire outer peripheral edge of the left side half of the lower surface portion 41 in the drawing.
- the bubble discharge portion 43 is provided in the pressing member 9 in this way, the meniscus is eliminated by the pressing member 9 and the retention of bubbles is also prevented. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 41a of the lower surface portion 41 is divided into two parts on the left and right sides of the figure, and has an oblique planar shape on both the left and right sides from the center of the figure (see angle ⁇ in FIG. 9). More specifically, the right side portion of the lower surface 41a is inclined so that the vicinity of the middle of the pair of first grooves 43a is the highest. The left side of the lower surface 41a is inclined so that the vicinity of the middle of the pair of second grooves 43b is the highest. Thereby, the bubbles near the lower surface 41a are guided along the lower surface 41a toward the pair of first grooves 43a and the pair of second grooves 43b.
- the cell culture medium C is injected into the recess 7.
- the amount of the cell culture solution C at this time is, for example, a position where the lower surface 41a of the pressing member 9 contacts the upper surface of the cell culture solution C.
- the pressing member 9 is fitted into the recess 7.
- the liquid surface of the cell culture solution C is pushed by the lower surface 41 a.
- a part of the cell culture solution C enters the first groove 43 a and the second groove 43 b of the bubble discharge part 43.
- the liquid level of the cell culture solution is located in the first groove 43a and the second groove 43b, but the cell culture solution C exceeds the first groove 43a and the second groove 43b.
- the portion may spill over the lower surface portion 41.
- the effect obtained in this embodiment is the same as the effect obtained in the first embodiment. Further, the number, position, and shape of the grooves as the bubble emitting part can be changed as necessary. Moreover, the bubble discharge
- the shape of the lower surface 23a is not limited to the said embodiment.
- the lower surface may be a plane that extends horizontally as a whole, or may be a plurality of inclined planes formed corresponding to each groove.
- FIG. 10 is a plan view of a part of the cell culture container.
- 11 is a cross-sectional view taken along the line XI-XI in FIG.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the pressing member 9 has a lower surface 45a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing portion 47 that allows the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble emitting portion 47 is a linearly extending groove formed at the left and right center of the lower surface portion 41. Specifically, the bubble discharge portion 47 extends from the outer peripheral edge of the lower surface portion 41 to the outer peripheral edge in the drawing.
- the bubble discharge portion 47 is provided on the pressing member 9 as described above, the meniscus is eliminated by the pressing member 9 and the retention of bubbles is also prevented. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 45a of the lower surface portion 45 is divided into two parts in the left and right direction in the figure, and has an oblique planar shape on both the left and right sides from the center in the left and right direction (see angle ⁇ in FIG. 11). More specifically, the right side portion of the lower surface 45a is inclined so that the vicinity of the bubble emitting portion 47 is the highest. The left side portion of the lower surface 45a is inclined so that the vicinity of the bubble emitting portion 47 is highest. As a result, bubbles in the vicinity of the lower surface 45a are guided toward the bubble discharge portion 47 along the lower surface 45a.
- the effects obtained in this embodiment are the same as the effects obtained in the first and second embodiments.
- the number, position, and shape of the grooves as the bubble emitting part can be changed as necessary.
- release part formed in the lower surface part may be hole shape instead of groove shape.
- the shape of the lower surface 45a is not limited to the above embodiment.
- the lower surface may be a flat surface that extends horizontally.
- FIG. 12 is a plan view of a part of the cell culture container. 13 is a sectional view taken along line XIII-XIII in FIG.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the pressing member 9 has a lower surface 49a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing portion 51 that enables the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble discharge portion 51 is a groove formed in an arc shape formed on the outer peripheral edge of the lower surface portion 49. Specifically, the bubble discharge part 51 has a pair of first grooves 51a on the right side of the figure. The pair of first grooves 51 a extends over the entire right half of the outer peripheral edge of the lower surface 49.
- the bubble discharge part 51 is provided in the pressing member 9 to prevent the bubbles from staying. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 49a of the lower surface portion 49 has a planar shape, but is not horizontal but inclined (see angle ⁇ in FIG. 13). More specifically, the lower surface 49a is inclined so that the vicinity of the middle between the pair of first grooves 51a of the bubble discharge portion 51 is the highest. As a result, the air bubbles in the vicinity of the lower surface 49a are guided toward the air bubble discharge portion 51 along the lower surface 49a.
- a liquid supply part 52 is formed on the pressing member 9.
- the liquid supply unit 52 is used to move the bubbles in the cell culture solution C toward the bubble discharge unit 51 by injecting the liquid into the cell culture solution C.
- the liquid supply part 52 is a groove formed in an arc shape formed on the outer peripheral edge of the lower surface part 49.
- the liquid supply unit 52 includes a pair of second grooves 52a on the left side of the drawing. The pair of second grooves 52 a extends over the entire left half of the outer peripheral edge of the lower surface portion 49 in the drawing.
- the liquid supply part 52 is formed at a position facing the bubble discharge part 51 in the radial direction. That is, the middle of the pair of second grooves 52a of the liquid supply unit 52 is the lowest position of the lower surface 49a.
- the liquid supply for example, a pipette is used.
- the effect obtained in this embodiment is the same as the effect obtained in the first to third embodiments.
- the inclination of the lower surface 49a may be omitted or may have another shape.
- the hydrophilic treatment of the lower surface 49a may be omitted or may be performed partially.
- the liquid supply part 52 may be omitted or may have another shape.
- FIG. 14 is a plan view of a part of the cell culture container.
- 15 is a cross-sectional view taken along the line XV-XV in FIG.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the pressing member 9 has a lower surface 53a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing portion 55 that allows the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble discharge portion 55 is a groove formed in an arc shape formed on the outer peripheral edge of the lower surface portion 53. Specifically, the bubble emitting part 55 is a groove extending along the outer peripheral edge of the lower half 53 on the right half of the figure.
- the bubble discharge portion 55 is provided on the pressing member 9 in this way, the meniscus is eliminated by the pressing member 9 and the retention of bubbles is also prevented. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 53a of the lower surface portion 53 has a planar shape, but is not horizontal but is inclined (see angle ⁇ in FIG. 15). More specifically, the lower surface 53a is inclined so that the vicinity of the middle part of the bubble discharge part 55 is highest. As a result, bubbles near the lower surface 53a are guided toward the bubble discharge portion 55 along the lower surface 53a.
- the effects obtained in this embodiment are the same as the effects obtained in the first to fourth embodiments.
- the number, position, and shape of the grooves as the bubble emitting part can be changed as necessary.
- release part formed in the lower surface part may be hole shape instead of groove shape.
- the shape of the lower surface of the lower surface portion is not limited to the above embodiment.
- the lower surface may be a flat surface that extends horizontally.
- FIG. 16 is a plan view of a part of the cell culture container. 17 is a cross-sectional view taken along the line XVII-XVII in FIG.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the pressing member 9 has a lower surface 57a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing portion 59 that allows the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble emitting portion 59 is a plurality of grooves formed in an arc shape formed on the outer peripheral edge of the lower surface portion 57. Specifically, a total of four grooves are formed.
- the bubble discharge portion 59 is provided in the pressing member 9 in this way, the meniscus is eliminated by the pressing member 9 and the retention of bubbles is also prevented. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 57a of the lower surface portion 57 is a flat end surface extending horizontally.
- any one of the plurality of bubble discharge portions 59 can be used as the liquid supply portion.
- the effects obtained in this embodiment are the same as the effects obtained in the first to fifth embodiments.
- the number, position, and shape of the grooves that are the bubble discharge portions can be changed as necessary.
- release part formed in the lower surface part may be hole shape instead of groove shape.
- FIG. 18 is a plan view of a part of the cell culture container.
- FIG. 19 is a sectional view taken along line XIX-XIX in FIG.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the pressing member 9 has a lower surface 61a (described later) that comes into contact with the upper surface of the cell culture medium C in the fitted state. Furthermore, the pressing member 9 has a bubble releasing portion 63 that allows the bubbles in the cell culture medium C to be discharged to the outside.
- the bubble discharge portion 63 is a recess formed by cutting out a part of the outer peripheral surface 25 a of the cylindrical portion 25, and the recess is formed from the lower surface 61 a of the lower surface portion 61 to the cell culture container 1. It extends to the outside.
- the flange portion 27 is notched linearly in plan view. Since the bubble discharge portion 63 is provided in the pressing member 9 in this way, the meniscus is eliminated by the pressing member 9 and the retention of bubbles is also prevented. Therefore, the cells can be observed accurately and over a wide area, that is, in a wide range up to the outer peripheral edge of the recess 7.
- the lower surface 61a of the lower surface portion 61 has a planar shape, but is not horizontal but oblique (see angle ⁇ in FIG. 19). More specifically, the lower surface 61a is inclined so that the vicinity of the bubble emitting portion 63 is highest. Thereby, the air bubbles in the vicinity of the lower surface 61a are guided toward the air bubble discharge portion 63 along the lower surface 61a.
- the effects obtained in this embodiment are the same as the effects obtained in the first to sixth embodiments.
- the lower surface portions 23, 53, and 63 are disposed at positions relatively distant from the bottom portion 17, but this position is not particularly limited.
- FIG. 20 is a cross-sectional view of the cell culture container.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the lower surface portion 23 ⁇ / b> A is disposed close to the bottom portion 17.
- the present invention is applied to a container for observing cells in a culture solution, but the present invention can also be applied to other types of containers.
- a cell culture container for spheroid formation will be described with reference to FIGS. 21 to 24 are sectional views of the cell culture container.
- FIG. 25 is a top view of the pressing member. The basic configuration of the pressing member and the recess is the same as that in the above embodiment. Hereinafter, different points will be mainly described.
- FIG. 21 shows the container body 103 of the cell culture container.
- the container body 103 is a container whose upper side is open and holds the cell culture solution C.
- the cell culture medium C contains a large number of seed cells c.
- a microwell member 131 is installed on the bottom 117 of the recess 107.
- the microwell member 131 is a member having a plurality of microwells 131a. Specifically, the microwell member 131 has a large number of microwells 131a on the upper surface (corresponding to the first portion).
- the diameter of the microwell 131a is, for example, 30 to 1500 ⁇ m, and preferably 50 to 300 ⁇ m.
- the depth of the microwell 131a is, for example, 30 to 1500 ⁇ m, and preferably 50 to 300 ⁇ m.
- the microwell member 131 is provided at the center of the bottom portion 117, and the upper surface thereof is disposed relatively far from the bottom portion 117. Therefore, the outer peripheral side of the microwell member 131 is a surface (corresponding to the second portion) lower than the upper surface of the microwell member 131 in the bottom portion 117.
- the pressing member 109 is inserted into the concave portion 107 of the container main body 103.
- the pressing member 109 has a disk-like lower surface portion 123.
- the lower surface 123a of the lower surface portion 123 is disposed close to the upper surface of the microwell member 131 (the portion where the microwell 131a is formed).
- the dimension between the lower surface 123a of the lower surface portion 123 and the upper surface of the microwell member 131 may be shorter than the diameter of the spheroid S, and is, for example, 0 to 300 ⁇ m.
- a plurality of bubble discharge portions 123 b are formed on the outer peripheral side of the microwell member 131.
- the bubble discharge part 123b is provided corresponding to the second part of the bottom part 117 (the part without the microwell 131a).
- the bubble discharge part 123b is a dot-like through hole. Therefore, bubbles in a space below the lower surface portion 123 (for example, around the plurality of microwells 131a and the microwell member 131) are easily discharged to the outside.
- seed cell c is seeded. Specifically, as shown in FIG. 21, the cell culture solution C is injected into the recess 107.
- the cell culture medium C is a suspension composed of a liquid medium and seed cells c that are uniformly diffused in the medium.
- the seed cell c has adhesiveness, and is, for example, a cancer cell such as a human osteosarcoma cell or a hepatocyte.
- As the medium a known medium suitable for culturing adherent cells is used. In the above case, each seed cell c descends to the microwell 131a and is landed.
- the pressing member 109 is fitted into the recess 107 as shown in FIG. And as shown in FIG. 23, the spheroid S is formed because the several seed cell c aggregates in the microwell 131a.
- the lower surface portion 123 is disposed above the plurality of microwells 131a in the recess 107, and movement is restricted so that the spheroid S grown in the microwell 131a does not separate from the microwell 131a. Yes. Therefore, the spheroid S is stably formed in the cell culture container.
- a new culture medium can be inject
- the nutrients and oxygen are supplied to the cells during the culture by the medium circulation, and the cells grow healthy.
- a large spheroid S requiring a relatively long culture period can be formed, and the formed spheroid S can be stored for a long time.
- the cell waste and waste which did not become spheroid S are removed by medium exchange.
- the cell culture solution C is reduced so that the cell culture solution C does not exist above the lower surface 123, and the spheroid S is observed from below the container body 103.
- the lower surface 123a of the lower surface portion 123 is in contact with the upper surface of the cell culture medium C, so that the meniscus is eliminated.
- the spheroid S grown in the plurality of microwells 131a can be accurately observed in the container body 103.
- the bubble emitting portion is not formed at the position corresponding to the plurality of microwells 131a on the lower surface portion 123, the spheroid S grown in the plurality of microwells 131a can be accurately observed.
- the bubble discharge portion is a plurality of holes, but the form of the bubble discharge portion is not limited thereto.
- FIG. 26 is a top view of the pressing member.
- the basic configuration of the pressing member and the recess is the same as that in the above embodiment.
- different points will be mainly described.
- the bubble discharge part 123c is an arc-shaped through groove extending in the circumferential direction.
- release part is not limited to the through-hole formed in a lower surface part.
- the bubble releasing part may be a notch, a slit, or a through hole formed between the cylindrical part 125 of the pressing member 109 and the cylindrical part 119 of the container main body 103. Since no bubble emitting part is formed at the position corresponding to the plurality of microwells 131a in the lower surface part 123 (that is, the central part and the intermediate part in the radial direction), the spheroid S grown in the plurality of microwells 131a can be accurately observed. .
- the lower surface portion of the pressing member has a flat shape, but the shape of the lower surface portion is not particularly limited.
- FIG. 27 is a cross-sectional view of the cell culture container. The basic configuration of the pressing member and the recess is the same as that in the above embodiment. Hereinafter, different points will be mainly described.
- the lower surface portion of the pressing member 109 has a flat pressing portion 123A and an annular protruding portion 123B.
- the holding portion 123 ⁇ / b> A is disposed in the vicinity of the upper surface of the microwell member 131.
- the annular protrusion 123B is formed on the outer peripheral edge of the pressing portion 123A and extends downward. That is, the protrusion 123 ⁇ / b> B is disposed so as to surround the outer peripheral side of the microwell member 131.
- a plurality of bubble discharge portions 123b are formed on the bottom surface of the protruding portion 123B.
- the shape of the bubble discharge part 123b is a dot-shaped or arc-shaped through-hole or a combination thereof.
- FIG. 28 is a cross-sectional view of the cell culture container.
- a lid 11 is disposed above the plate 5 with a pressing member.
- the lid 11 covers the entire upper side of the plate 5 with a pressing member.
- the lid 11 has a flat main body 11a and a cylindrical portion 11b extending downward from the outer peripheral edge thereof.
- the cushion member 12 is disposed between the main body 11a of the lid 11 and the flange portion 27 of the plate 5 with the pressing member.
- the cushion member 12 is an elastic member such as a spring, rubber, or sponge.
- the cushion member 12 generates an elastic force by being compressed between the plate 5 with a pressing member and the lid 11. Thereby, it is prevented that the board 5 with a pressing member floats, and it can fix in the container main body 3.
- FIG. Thereby, for example, even when spheroids are formed, the gap between the microwell and the mesh can be sufficiently shortened, thereby obtaining a spheroid fixing effect.
- FIG. 29 is a cross-sectional view of a cell culture container.
- the basic structure is the same as in the twelfth embodiment.
- a first engagement portion 15 is formed on the outer peripheral portion of the container body 3, and a second engagement portion 16 is formed on the cylindrical portion 11 b of the lid 11.
- the first engaging portion 15 and the second engaging portion 16 are engaged with each other, so that the lid 11 is not separated from the container body 3.
- the cushion member 12 generates an elastic force by being compressed between the plate 5 with a pressing member and the lid 11.
- plate 5 with a pressing member is prevented from floating, and can be fixed in the container main body 3.
- the gap between the microwell and the mesh can be sufficiently shortened, thereby obtaining a spheroid fixing effect.
- the lift of the plate 5 with a pressing member is prevented by using a lid and a cushion member, but the lift can be prevented by other means.
- the plate 5 with a pressing member itself is made heavy by using a metal material in part.
- a weight is attached to a part of the plate 5 with the pressing member.
- FIG. 30 is a perspective view of the container body of the cell culture container.
- the basic structure is the same as in the first to fourteenth embodiments. Hereinafter, different points will be mainly described.
- a plurality of pins 71 are erected at the upper corner of the container body 3. Specifically, the pins 71 are arranged at the four corners of the outer portion of the main body portion where the concave portion 7 of the container main body 3 is formed.
- a hole 73 is formed in the plate 5 with a pressing member at a position corresponding to the pin 71. Specifically, the holes 73 are formed at the four corners of the frame 5b.
- the plurality of pressing members 9 are smoothly fitted into the recesses 7 by positioning with the pins 71 and the holes 73.
- the number of pins and holes is not limited to the above embodiment.
- the positioning structure of the container main body 3 and the plate 5 with a pressing member is not limited to pins and holes.
- the culture container (for example, the cell culture container 1) includes a container body (for example, the container body 3 and the container body 103) and a pressing member (for example, the pressing member 9 and the pressing member 109).
- the container body has a recess (for example, the recess 7 and the recess 107) for accommodating a culture solution (for example, cell culture solution C).
- the pressing member is a member that is detachably fitted to the upper side of the recess.
- the pressing member has a lower surface (for example, a lower surface 23a, a lower surface 41a, a lower surface 45a, a lower surface 49a, a lower surface 53a, a lower surface 57a, a lower surface 61a, and a lower surface 123a) that is in contact with the upper surface of the culture solution,
- a bubble emitting unit that enables the bubbles to be discharged to the outside (for example, bubble emitting unit 13, bubble emitting unit 43, bubble emitting unit 47, bubble emitting unit 51, bubble emitting unit 55, bubble emitting unit 59, bubble emitting unit 63, bubble A discharge portion 123b and a bubble discharge portion 123c).
- the pressing member is fitted on the upper side of the recess while the culture solution is contained in the recess of the container body. Then, the lower surface of the pressing member comes into contact with the upper surface of the culture solution, thereby eliminating the meniscus. In this state, the bubbles in the culture solution are discharged to the outside from the bubble discharge portion of the pressing member. As a result, the cells can be accurately observed in the culture container.
- the shape of the concave portion and the pressing member in a plan view and the combination thereof are not limited to the above embodiment.
- the number of recesses and pressing members is not limited to the above embodiment.
- the hydrophilic treatment of the lower surface of the pressing member may be performed partially or entirely, or may not necessarily be performed.
- the lower surface of the pressing member may be an inclined surface, may be performed partially or entirely, or may not necessarily be performed.
- the cell culture container using the cell culture solution has been described as an example of the culture container. However, for example, animal cells, plant cells, fungi, and bacteria can be cultured in the culture container according to the present invention.
- the present invention can be widely applied to a culture vessel that contains a culture solution used for microscopic observation.
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Abstract
Description
また、目的に合った容器を用いて培養を行うために、様々な形状及びサイズの培養容器が開発されている。
そのような問題を解決するために、特許文献1に記載の細胞培養容器では、容器の中にメニスカス制御用平板を浮かせている。
容器本体は、培養液を収容するための凹部を有する。
押さえ部材は、凹部の上側に取り外し可能に嵌められる部材である。押さえ部材は、嵌められた状態で培養液の上面に当接する下面と、培養液内の気泡を外部に放出可能にする気泡放出部とを有する。
この容器では、容器本体の凹部に培養液を収容した状態で、押さえ部材が凹部の上側に嵌められる。すると、押さえ部材の下面が培養液の上面に当接することで、メニスカスが解消される。この状態で、培養液内の気泡は、押さえ部材の気泡放出部から外部に放出される。この結果、培養容器において細胞を正確に観察できる。
この容器では、培養液内の気泡は、押さえ部材の下面の傾斜面によって気泡放出部に誘導される。したがって、押さえ部材の下面近傍に気泡が滞留しにくい。
押さえ部材の下面は、少なくとも部分的に親水化処理されていてもよい。
この容器では、押さえ部材の下面近傍に気泡が滞留しにくい。
この容器では、液体が液体供給部から培養液内に注入されると、培養液内の気泡が気泡放出部側に移動させられる。したがって、押さえ部材の下面近傍に気泡が滞留しにくい。
凹部の底部は、複数のマイクロウェルが形成された第1部分を有していてもよい。その場合、押さえ部材の下面は、培養液の上面に当接する。
メニスカスが防止されることにより、複数のマイクロウェルで成長したスフェロイドを正確に観察できる。
底部において複数のマイクロウェルに対応する位置には気泡放出部が形成されていないので、複数のマイクロウェルで成長したスフェロイドを正確に観察できる。
(1)全体構造
図1~図3を用いて、本発明の一実施形態としての培養容器としての細胞培養容器1を説明する。
図1は、細胞培養容器の容器本体の斜視図である。図2及び図3は、細胞培養容器の押さえ部材付き板の斜視図である。
押さえ部材付き板5は、容器本体3に対応する長方形形状であり、凹部7に対応する複数の押さえ部材9を有している。押さえ部材付き板5は、肉厚は薄く、透明性の部材であり、例えば透明プラスチック製である。押さえ部材付き板5は、平板状の本体5aと、その外周面に形成された枠5bとを有している。押さえ部材付き板5の本体5aには、複数の押さえ部材9が設けられている。押さえ部材9は、凹部7の上側に取り外し可能に嵌め込まれる部材である。
なお、図2に示す押さえ部材付き板5は6×4合計24個の押さえ部材9を有しており、図3に示す押さえ部材付き板5Aは6×1合計6個の押さえ部材9を有している。図3に示す押さえ部材付き板5Aは、図1に示す容器本体3に対しては4個が用いられる。
細胞培養容器1における細胞の観察は、各凹部7の下方から各種顕微鏡を用いて行われる。具体的には、細胞は凹部7の底部に接着した状態になっていることが多いので、そのような状態で細胞は観察される。ただし、浮遊培養を行う場合は、細胞培養液C内を浮遊している細胞を観察することもある。
次に、図5~図7を用いて、凹部7と押さえ部材9の構造及び両者の関係を詳細に説明する。図5は、細胞培養容器の一部の平面図である。図6は図5のVI-VI断面図であり、図7は図5のVII-VII断面図である。
このように気泡放出部13が押さえ部材9に設けられていることで、押さえ部材9によってメニスカスを解消すると共に、気泡の滞留も防止している。したがって、正確にかつ広い面積にわたって、つまり凹部7の外周縁までの広い範囲で、細胞を観察できる。
なお、下面23aの傾斜角度θは、1~30度であり、より好ましくは1~5度である。
さらに、気泡放出部13及び液体供給部31は、ピペットによる差し込み播種を可能にしている。これにより、押さえ部材9をかぶせた後に、細胞全体の数量及び成長状況を調整できる。
従来であれば、例えば直径7mm程度の凹部においてメニスカスの影響を受けずに観察できる範囲は直径1~3mm程度であったが、本実施形態では押さえ部材9の肉厚を除いて直径5mm程度の範囲で観察可能になる。これにより、従来では推察によって判断していた領域の確認ができるようになり、正確な経時変化及び状態観察が可能になる。
さらに、気泡放出部13によって、細胞培養液C内の気泡を細胞培養容器1の外部に放出できる。また、下面23aの傾斜及び親水化処理、さらには液体供給部31によって、気泡の排出が確実かつ速やかに行われるようになっている。
一般的には、容器本体の種類としては、6、12、24、48、96、384ウェルがある。24ウェルの場合は、押さえ部材の内径は8.0~14.0mmであり、押さえ部材の縦方向長さ(フランジを除く)は3.0~10.0mmであり、押さえ部材の外径は8.0~14.0mmであり、凹部の底面内径は14.0~17.0mmである。96ウェルの場合は、押さえ部材の内径は4.0~5.0mmであり、押さえ部材の縦方向長さ(フランジを除く)は3.0~10.0mmであり、押さえ部材の外径は4.0~5.0mmであり、凹部の底面内径は5.0~7.0mmである。
以下、図8及び図9を用いて、第2実施形態を説明する。図8は、細胞培養容器の一部の平面図である。図9は、図8のIX-IX断面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
また、気泡放出部としての溝の数、位置、形状は必要に応じて変更可能である。また、下面部に形成された気泡放出部は、溝形状ではなく、孔形状であってもよい。
下面23aの形状は前記実施形態に限定されない。下面は、全体が水平に延びる平面であってもよいし、各溝に対応して形成された複数の傾斜平面であってもよい。
以下、図10及び図11を用いて、第3実施形態を説明する。図10は、細胞培養容器の一部の平面図である。図11は、図10のXI-XI断面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
この実施形態で得られる効果は、第1~第2実施形態で得られる効果と同じである。
下面45aの形状は前記実施形態に限定されない。下面は、全体が水平に延びる平面であってもよい。
以下、図12及び図13を用いて、第4実施形態を説明する。図12は、細胞培養容器の一部の平面図である。図13は、図12のXIII-XIII断面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
なお、下面49aの傾斜は、省略されてもよいし、他の形状であってもよい。また、下面49aの親水化処理は、省略されてもよいし、部分的に行われてもよい。さらには、液体供給部52は、省略されてもよいし、他の形状であってもよい。
以下、図14及び図15を用いて、第5実施形態を説明する。図14は、細胞培養容器の一部の平面図である。図15は、図14のXV-XV断面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
気泡放出部としての溝の数、位置、形状は必要に応じて変更可能である。また、下面部に形成された気泡放出部は、溝形状ではなく、孔形状であってもよい。
下面部の下面の形状は前記実施形態に限定されない。下面は、全体が水平に延びる平面であってもよい。
以下、図16及び図17を用いて、第6実施形態を説明する。図16は、細胞培養容器の一部の平面図である。図17は、図16のXVII-XVII断面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
なお、この実施形態では、複数の気泡放出部59のいずれかを液体供給部として用いることができる。
また、気泡放出部である溝の数、位置、形状は必要に応じて変更可能である。また、下面部に形成された気泡放出部は、溝形状ではなく、孔形状であってもよい。
次に、図18及び図19を用いて、第7実施形態を説明する。図18は、細胞培養容器の一部の平面図である。図19は図18のXIX-XIX断面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
このように気泡放出部63が押さえ部材9に設けられていることで、押さえ部材9によってメニスカスを解消すると共に、気泡の滞留も防止している。したがって、正確にかつ広い面積にわたって、つまり凹部7の外周縁までの広い範囲で、細胞を観察できる。
この実施形態で得られる効果は、第1~第6実施形態で得られる効果と同じである。
第1~第7実施形態では、下面部23、53,63は、底部17から比較的離れた位置に配置されていたが、この位置は特に限定されない。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
図から明らかなように、下面部23Aは、底部17に近接して配置されている。この構造は、第1~第8実施形態のいずれにも適用可能であり、それら実施形態と同じ効果が得られる。
第1~第8実施形態では培養液内の細胞を観察する容器に本発明を適用していたが、本発明は他の種類の容器にも適用可能である。
図21~図24を用いて、スフェロイド形成用の細胞培養容器を説明する。図21~図24は細胞培養容器の断面図である。図25は、押さえ部材の上面図である。
なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
凹部107の底部117には、マイクロウェル部材131が設置されている。マイクロウェル部材131は、複数のマイクロウェル131aを有する部材である。具体的には、マイクロウェル部材131は、上面(第1部分に相当)に多数のマイクロウェル131aを有している。マイクロウェル131aの径は、例えば30~1500μmであり、好ましくは50~300μmである。マイクロウェル131aの深さは、例えば、30~1500μmであり、好ましくは50~300μmである。
この実施形態では、マイクロウェル部材131は底部117の中心に設けられ、その上面は底部117から比較的離れた上方に配置されている。そのため、底部117においてマイクロウェル部材131の外周側はマイクロウェル部材131の上面より低い面(第2部分に相当)となっている。
そして、図23に示すように、マイクロウェル131a内で、複数の種細胞cが凝集することで、スフェロイドSを形成する。この細胞培養容器では、下面部123が、凹部107内で複数のマイクロウェル131aの上方に配置され、マイクロウェル131a内で成長したスフェロイドSが当該マイクロウェル131aから離脱しないように移動を制限している。したがって、細胞培養容器においてスフェロイドSが安定的に形成される。
上記の培地交換において、凹部107内において培地の流れが生じるが、このとき、下面部123によってスフェロイドSを押さえているので、スフェロイドSがマイクロウェル131aから飛び出さない。したがって、スフェロイドSの流出が防止されている。
第9実施形態では気泡放出部は複数の孔であったが、気泡放出部の形態はそれに限定されない。
図26を用いて、押さえ部材の他の実施形態を説明する。図26は、押さえ部材の上面図である。なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
下面部123において、マイクロウェル部材131よりさらに外周側の部分には複数の気泡放出部123cが形成されている。気泡放出部123cは、円周方向に延びる弧状の貫通溝である。
下面部123において複数のマイクロウェル131aに対応する位置(つまり中心部及び半径方向中間部)には気泡放出部が形成されていないので、複数のマイクロウェル131aで成長したスフェロイドSを正確に観察できる。
第9実施形態では押さえ部材の下面部は平坦な形状であったが、下面部の形状は特に限定されない。
図27を用いて、押さえ部材109の他の実施形態を説明する。図27は、細胞培養容器の断面図である。なお、押さえ部材及び凹部の基本的な構成は前記実施形態と同じである。以下、異なる点を中心に説明する。
下面部において複数のマイクロウェル131aに対応する位置(つまり、押さえ部123A)には気泡放出部が形成されていないので、複数のマイクロウェル131aで成長したスフェロイドSを正確に観察できる。
特に、第8~第11実施形態では、押さえ部材の下面部とその下方の部材との間の寸法を正確に管理することが求められる。しかし、押さえ部材は培養容器の培地に押し込んで使用されるので、浮力で押さえ部材が浮いてしまうという問題が考えられる。
そこで、図28を用いて、そのような課題を解決するための構造を説明する。図28は、細胞培養容器の断面図である。
第12実施形態では、蓋は自重のみによって弾性部材を圧縮していたが、他の構造によって弾性部材を圧縮してもよい。
そこで、図29を用いて、そのような課題を解決するための構造を説明する。図29は、細胞培養容器の断面図である。
この実施形態では、容器本体3の外周部に第1係合部15が形成され、さらに蓋11の筒状部11bに第2係合部16が形成されている。第1係合部15と第2係合部16は互いに係合しており、それにより蓋11が容器本体3から離れないようになっている。
この実施形態では、クッション部材12は、押さえ部材付き板5と蓋11との間で圧縮されることで、弾性力を発生する。これにより、押さえ部材付き板5の浮きが防止され、容器本体3内に固定できる。これにより、例えば、スフェロイドを形成するような場合でも、マイクロウェルとメッシュとの間の隙間を十分に短くでき、それによりスフェロイド固定効果が得られる。
第12実施形態及び第13実施形態では、蓋及びクッション部材を用いることで押さえ部材付き板5の浮き上がりを防止していたが、他の手段によっても浮き上がり防止は可能である。例えば、押さえ部材付き板5の重量を増加することで、浮力による浮きを防止できる。具体的には、金属製材料を一部に使うことでで、押さえ部材付き板5自体を重くする。又は、押さえ部材付き板5の一部に錘を付ける。
複数の押さえ部材が複数の凹部にスムーズに嵌まるように両者に位置決め構造を設けた実施形態を説明する。
なお、基本的な構造は第1~第14実施形態と同じである。以下、異なる点を中心に説明する。
押さえ部材付き板5には、ピン71に対応する位置に孔73が形成されている。具体的には、孔73は、枠5bの四隅に形成されている。
なお、ピンと孔の数は前記実施形態に限定されない。さらに、容器本体3と押さえ部材付き板5との位置決め構造は、ピンと孔に限定されない。
上記第1~第15実施形態は、下記の構成及び機能を共通に有している。
培養容器(例えば、細胞培養容器1)は、容器本体(例えば、容器本体3,容器本体103)と、押さえ部材(例えば、押さえ部材9、押さえ部材109)とを備えている。 容器本体は、培養液(例えば、細胞培養液C)を収容するための凹部(例えば、凹部7、凹部107)を有する。
以上、本発明の複数の実施形態について説明したが、本発明は上記実施形態に限定されるものではなく、発明の要旨を逸脱しない範囲で種々の変更が可能である。特に、本明細書に書かれた複数の実施形態及び変形例は必要に応じて任意に組み合せ可能である。
凹部及び押さえ部材の個数は、前記実施形態に限定されない。
押さえ部材の下面を傾斜面にすること、部分的に又は全面的に行われていてもよいし、必ずしも行われなくてもよい。
前記実施形態では、培養容器の一例として細胞培養液を用いた細胞培養容器を説明した。しかし、本発明に係る培養容器では、例えば、動物細胞、植物細胞、菌、細菌も培養可能である。
3 :容器本体
5 :押さえ部材付き板
7 :凹部
9 :押さえ部材
13 :気泡放出部
17 :底部
19 :筒状部
19a :内周面
23 :下面部
23a :下面
25 :筒状部
25a :外周面
27 :フランジ部
31 :液体供給部
Claims (6)
- 培養液を収容するための凹部を有する容器本体と、
前記凹部の上側に取り外し可能に嵌められる部材であって、嵌められた状態で前記培養液の上面に当接する下面と、前記培養液内の気泡を外部に放出可能にする気泡放出部とを有する押さえ部材と、
を備えた培養容器。 - 前記押さえ部材の前記下面は、前記培養液内の気泡を前記気泡放出部に向けて誘導するための傾斜面を少なくとも部分的に有している、請求項1に記載の培養容器。
- 前記押さえ部材の前記下面は少なくとも部分的に親水化処理されている、請求項1又は2に記載の培養容器。
- 前記押さえ部材には、液体を前記培養液内に注入することで前記培養液内の気泡を前記気泡放出部側に移動させるのに用いられる液体供給部がさらに形成されている、請求項1~3のいずれかに記載の培養容器。
- 前記凹部の底部は、複数のマイクロウェルが形成された第1部分を有しており、
前記押さえ部材の前記下面は、前記培養液の上面に当接する、請求項1に記載の培養容器。 - 前記凹部の底部は、前記複数のマイクロウェルが形成されていない第2部分を有しており、
前記押さえ部材の前記気泡放出部は前記第2部分に対応して設けられている、請求項5に記載の培養容器。
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