WO2022146028A1 - 배지로부터 기포를 제거하기 위한 기포제거장치 및 이를 포함하는 배지순환식 세포배양 시스템 - Google Patents
배지로부터 기포를 제거하기 위한 기포제거장치 및 이를 포함하는 배지순환식 세포배양 시스템 Download PDFInfo
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- WO2022146028A1 WO2022146028A1 PCT/KR2021/020167 KR2021020167W WO2022146028A1 WO 2022146028 A1 WO2022146028 A1 WO 2022146028A1 KR 2021020167 W KR2021020167 W KR 2021020167W WO 2022146028 A1 WO2022146028 A1 WO 2022146028A1
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- WO
- WIPO (PCT)
- Prior art keywords
- medium
- space
- bubble removal
- removal device
- housing
- Prior art date
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- 238000004113 cell culture Methods 0.000 title claims description 58
- 210000003040 circulating cell Anatomy 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000002609 medium Substances 0.000 claims description 169
- 238000010899 nucleation Methods 0.000 claims description 28
- 239000001963 growth medium Substances 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 56
- 230000001464 adherent effect Effects 0.000 description 4
- 238000007599 discharging Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000254137 Cicadidae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D19/00—Degasification of liquids
- B01D19/0063—Regulation, control including valves and floats
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D19/00—Degasification of liquids
- B01D19/0042—Degasification of liquids modifying the liquid flow
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/21—Froth suppressors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D19/00—Degasification of liquids
- B01D19/02—Foam dispersion or prevention
Definitions
- the present invention relates to a bubble removal device for removing bubbles from a medium and a medium circulation type cell culture system including the same.
- Cell culture refers to culturing and propagating tissue pieces by supplying nutrients to the tissue pieces removed from the individual of multicellular organisms in a container.
- Animal cells derived from human or animal tissue may be cultured while suspended in a medium or attached to a support.
- Mainly blood cell-derived cells including hematopoietic stem cells
- cells derived from tissues such as skin, liver or lungs, and embryonic stem cells or mesenchymal stem cells, etc. are adherent cells.
- Floating cells can be proliferated alone in a state in which cells are suspended in a medium, but adherent cells can proliferate only in a state attached to the surface of the support.
- the adherent cells in order for the adherent cells to be smoothly cultured, it is important that the cells to be cultured are stably attached to the surface of the support.
- the seeding process of attaching these adherent cells to the surface of the support is performed by injecting a medium containing the cells to be cultured into the interior of the container in which the support is disposed. That is, the cultured cells included in the medium adhere to the surface of the support when the medium comes into contact with the surface of the support.
- the air bubbles contained in the medium prevent the cells from being stably attached to the surface of the support.
- the cells are cultured in a state in which the cells are not stably attached to the surface of the support, there is a problem in that the cells cannot be stably cultured.
- air bubbles contained in the medium need to be removed not only in the seeding process of attaching the cells to the surface of the support but also in the process of culturing the cells.
- the present invention was devised in view of the above points, and a bubble removal device for removing bubbles from a medium capable of supplying a medium in a bubble-removed state to the support side of the cell culture device, and a medium circulation type cell culture including the same
- the purpose is to provide a system.
- the present invention provides an apparatus for removing air bubbles from a medium, an internal space for storing a predetermined amount of the medium, an inlet through which the medium is introduced from the outside into the inner space, and bubbles in the inner space a housing including an outlet for discharging the medium in the removed state; and a support part coupled to the housing so as to maintain the housing at a predetermined height from the bottom surface, wherein the inner space includes a first space filled with the medium and an upper portion of the first space. and a second space in which the medium is not filled, wherein the first space and the second space are air bubbles for removing air bubbles from the medium divided through the water level of the medium introduced into the inner space through the inlet from the outside
- a removal device is provided.
- the inlet may be provided in the housing to be positioned at a position relatively higher than the outlet, and the outlet may be provided in the housing to be positioned in communication with the first space, and the inlet is It may be provided in the housing to be positioned at a position communicating with the second space.
- the second space may be a bubble separation space in which bubbles are separated while the medium flowing into the inner space through the inlet falls into the first space.
- the outlet may be provided in the housing so as to be located on the bottom surface of the inner space.
- the bubble removal device for removing bubbles from the medium may further include a vent hole provided in the housing to communicate with the second space.
- the bubble removing device for removing air bubbles from the medium includes a pressure regulating means coupled to the vent so as to control the amount of the medium stored in the inner space by adjusting the internal pressure of the inner space.
- a pressure regulating means coupled to the vent so as to control the amount of the medium stored in the inner space by adjusting the internal pressure of the inner space. may include more.
- the housing may include a housing-shaped housing body having the inner space, and a cover member detachably coupled to the housing body to cover the open upper portion of the inner space.
- the bubble removal device for removing bubbles from the medium may further include a sensing unit including a sensor for detecting the state of the medium stored in a predetermined amount in the inner space.
- the senor may include at least one of a PH sensor for detecting the PH of the medium and a temperature sensor for measuring the temperature of the medium.
- the senor may be arranged so that a partial length including one end is submerged in the medium filled in the first space.
- the sensing unit may be detachably coupled to one side of the housing.
- the medium may be a seeding medium containing cells to be cultured or a culture medium containing nutrients.
- the present invention is a cell culture apparatus in which at least one support to which the cells to be cultured are installed; a medium supply device in which a predetermined amount of a culture medium for culturing the cells is stored therein; a cell supply source in which a certain amount of a seeding medium containing cells to be attached to the support is stored; a bubble removal device for removing air bubbles from the culture medium introduced from the medium supply device or from the seeding medium introduced from the cell supply source and then supplying to the cell culture device side; and a pump for circulating the culture medium, wherein the bubble removal device provides a medium circulation type cell culture system, which is a bubble removal device for removing bubbles from the above-described medium.
- the seeding medium or culture medium may be supplied to the support included in the cell culture apparatus in a state in which air bubbles are removed.
- the bubble removal device for removing air bubbles from the medium of the present invention is used, cells can be stably attached to the surface of the support and the cells to be cultured can be smoothly supplied with nutrients from the medium, resulting in stable cell culture. may be possible
- FIG. 1 is a schematic view showing a bubble removal device for removing bubbles from a medium according to an embodiment of the present invention
- FIG. 2 is a view showing a state in which the main components are separated from FIG. 1;
- FIG. 3 is a cutaway view of the housing of FIG. 1;
- Figure 4 is a view showing a state in which the medium is filled in the inner space in Figure 3, and,
- FIG. 5 is a schematic diagram showing a medium circulation type cell culture system to which a bubble removal device for removing bubbles from a medium according to an embodiment of the present invention is applied.
- the bubble removal device 100 (hereinafter, referred to as 'bubble removal device') for removing bubbles from the medium according to an embodiment of the present invention includes a housing 110 and a support unit 120 as shown in FIG. 1 . It can be done, and the medium introduced to the inside can be discharged to the outside in a state in which air bubbles are removed.
- the medium may be a seeding medium containing the cells to be cultured, or may be a culture medium containing nutrients required for cell culture.
- the seeding medium may be a medium in which the cells to be cultured are included, and the culture medium may be a medium in which the cells to be cultured are not included while containing nutrients necessary for culturing the cells.
- the bubble removal apparatus 100 may be applied to a medium circulation type cell culture system 1000 in which a medium is circulated as shown in FIG. 5 .
- the seeding medium containing the cells to be cultured may be supplied from the cell supply source 200 to the cell culture apparatus 400 side, and the cells included in the seeding medium are provided in the cell culture apparatus 400 . It may be attached to the surface of a supported support (not shown).
- the culture medium containing nutrients required for cell culture may be sequentially circulated through the cell culture device 400 , the bubble removal device 100 , and the medium supply device 300 by driving the pump 500 .
- the bubble removal device 100 removes bubbles from the culture medium introduced from the medium supply device 300 or the seeding medium introduced from the cell supply source 200, and then the bubbles are removed from the culture medium or seeds.
- Ding medium may be supplied to the cell culture device 400 side.
- the cells included in the seeding medium can be stably attached to the surface of the support (not shown) provided in the cell culture apparatus 400, and the cells attached to the support are supplied from the culture medium. It can be cultured smoothly through nutrients.
- the housing 110 may include an internal space 113 of a predetermined volume so that a predetermined amount of the medium supplied from the outside can be stored, and the housing 110 receives the medium from the outside into the internal space 113 .
- ) may include an inlet 114 for inflow and an outlet 115 for discharging the medium stored in the inner space 113 to the outside.
- the inlet 114 may be connected to the medium supply device 300 or the cell supply source 200 through the connection pipe 810 , and the outlet 115 may supply the medium through the connection pipe 820 . It may be connected to the device 300 .
- the culture medium introduced from the medium supply device 300 through the inlet 114 and the connection pipe 810 or the seeding medium introduced from the cell supply source 200 is placed in the inner space 113 .
- a certain amount may be stored, and the medium stored in the inner space 113 may be discharged to the outside through the outlet 115 .
- the medium discharged through the outlet 115 may move along the connection tube 820 to the cell culture apparatus 400 .
- the housing 110 is a housing body 111 having a housing shape having the inner space 113 and one side of the housing body 111 so as to cover the open upper part of the inner space 113 . It may include a cover member 112 that is detachably coupled to the.
- the inlet 114 may be provided in the housing body 111 to communicate with the inner space 113
- the outlet 115 may be provided in the housing body 111 to communicate with the inner space 113 . 111) may be provided.
- cover member 112 may be detachably coupled to the housing body 111 through at least one fastening member 118 , 119 .
- the fastening members 118 and 119 may include at least one of a first fastening member 118 and a second fastening member 119, and the first fastening member 118 may be a known bolt member. and the second fastening member 119 may be a known toggle clamp.
- the toggle clamp may be a commercial name cicadas.
- the cover member 112 is coupled to the housing body 111 through at least one of the first fastening member 118 and the second fastening member 119 to open the inner space 113 .
- the upper part can be sealed.
- the medium introduced into the inner space 113 in the bubble removing device 100 may be discharged to the outside through the outlet 115 after the bubbles are removed.
- the inner space 113 may be divided into a first space 113a filled with the medium and a second space 113b not filled with the medium, as shown in FIG. 4 , and the first The space 113a and the second space 113b may be divided through the water level of the medium introduced into the inner space 113, and the second space 113b is located on the upper side of the first space 113a. It can be formed to be positioned.
- the inlet 114 may be provided in the housing 110 to be positioned at a relatively higher position than the outlet 115 .
- the outlet 115 may be provided in the housing 110 so as to communicate with the first space 113a formed on the lower side of the inner space 113 , and the inlet 114 may be formed in the inner space. It may be provided in the housing 110 so as to communicate with the second space 113b located on the upper side of 113 .
- the outlet 115 may be provided in the housing 110 to communicate with the first space 113a while being positioned on the bottom surface of the inner space 113 .
- the medium introduced into the inner space 113 from the outside through the inlet 114 may move to the first space 113a through the second space 113b, and the first space 113a ) stored in the medium may be discharged to the outside through the outlet (115).
- the second space 113b may serve as a bubble separation space for separating bubbles from the medium flowing into the inner space 113 through the inlet 114 .
- the medium introduced into the inner space 113 from the outside through the inlet 114 may fall along the second space 113b, and air bubbles may be separated, and the air bubbles in the second space 113b may be separated.
- the separated medium may move to the first space 113a.
- the medium introduced into the inner space 113 can move to the first space 113a after air bubbles are removed from the second space 113b, and the medium filled in the first space 113a is It may be discharged to the outside in a state in which air bubbles are removed through the outlet 115 .
- the medium supplied to the cell culture apparatus 400 may be a medium in which air bubbles are completely removed.
- the medium in a bubble-free state can be supplied to the cell culture device 400 side, so the cells included in the seeding medium are It can be stably attached to the surface of the support.
- the cells attached to the surface of the support can receive nutrients from the medium while receiving minimal stress.
- the bubble removal apparatus 100 may adjust the amount of the medium stored in the inner space 113 by adjusting the internal pressure of the inner space 113 .
- the bubble removal device 100 may include a vent hole 116 provided in the housing 110 to communicate with the inner space 113 , and the vent hole 116 is a pressure adjusting means ( 130) can be connected.
- vent hole 116 may be provided in the cover member 112 , and the vent hole 116 may communicate with the second space 113b.
- the pressure regulating means 130 may include a vent filter 131 , and may serve as a vent means for discharging air bubbles separated from the medium and filled in the second space 113b to the outside. have.
- the bubble removing device 100 can adjust the water level of the medium flowing into the inner space 113 through the opening and closing of the pressure adjusting means 130, and the inner space ( 113) may be divided into the first space 113a and the second space 113b through the water level of the medium.
- the level of the medium introduced into the inner space 113 may be changed or maintained through opening and closing of the pressure adjusting means 130 .
- the level of the medium introduced into the inner space 113 may be adjusted through the pressure adjusting means 130 to be maintained at a position relatively lower than the position of the inlet 114 .
- the medium introduced into the inner space 113 through the inlet 114 can always move to the first space 113a through the second space 113b, and at the inlet 114,
- the medium moving to the first space 113a may be a medium in which air bubbles are separated from the second space 113b.
- the medium discharged to the outside through the outlet 115 may always be in a state in which air bubbles are removed.
- the support part 120 may support the housing 110 while being spaced apart from the bottom by a predetermined height.
- the support part 120 may include a bottom plate 122 and a side plate 124 extending at a predetermined height from the bottom plate 122 , and the housing 110 has one side on the side plate 124 . can be combined.
- the outlet 115 may be disposed to be positioned on the lower side of the housing 110 while the housing 110 is coupled to the support 120 .
- the housing 110 may be coupled to the support 120 such that the inlet 114 is positioned at a relatively higher position than the outlet 115 , and the outlet 115 is the housing 110 . Even if it is formed on the lower side, it may be arranged to maintain a state spaced apart from the bottom plate 122 by a predetermined height.
- the outlet 115 may communicate with the first space 113a. and the inlet 114 may communicate with the second space 113b.
- the shape of the support part 120 is not limited thereto, and as long as it has a shape that can separate the housing 110 from the bottom by a predetermined height, it may be appropriately changed according to design conditions.
- the bubble removal apparatus 100 may further include a sensing unit 140 including sensors 143 and 144 for detecting the state of the medium stored in the inner space 113 . .
- the sensors 143 and 144 may include at least one of a PH sensor 143 for detecting the PH of the medium and a temperature sensor 144 for measuring the temperature of the medium.
- the sensors 143 and 144 may be provided in the housing 110 so that at least a part of the medium and the medium stored in the inner space 113 are in contact.
- the sensors 143 and 144 may be disposed so that a partial length including one end is submerged in the medium filled in the first space 113a.
- the bubble removal apparatus 100 can monitor the state of the medium before being supplied to the cell culture apparatus 400 through the sensors 143 and 144, so that the outlet ( 115), it is possible to maintain the medium supplied to the cell culture apparatus 400 in an optimal state.
- the sensing unit 140 may be detachably coupled to one side of the housing 110 .
- the sensing unit 140 may include a stopper 141 that is detachably coupled to the housing 110 as shown in FIGS. 2 and 3, and the stopper 141 is the sensor ( It may include at least one coupling hole 142 for fixing the 143,144.
- the stopper 141 may be detachably coupled to the fastening hole 117 formed through the cover member 112 , and the sensors 143 and 144 may be detachably coupled to the coupling hole 142 .
- sensors 143 and 144 may be connected to a controller (not shown) through a cable.
- the user can monitor and manage the status of the badge based on the information measured through the sensors 143 and 144 .
- the type of the sensor is not limited thereto, and various known sensors may be applied as long as it can measure information on the state of the medium required for cell culture.
- the bubble removal apparatus 100 may be applied to the medium circulation type cell culture system 1000 as described above.
- the medium circulation type cell culture system 1000 includes a cell culture device 400 , a medium supply device 300 , a cell supply source 200 , and bubble removal. It may include device 100 and pump 500 .
- the medium supply device 300 stores a certain amount of culture medium required for cell culture.
- the cell supply source 200 may store a certain amount of the seeding medium required for cell culture.
- the seeding medium stored therein may be moved to the bubble removal device 100 side through the injection of a gas having a predetermined carbon dioxide concentration, and the culture medium is the medium supply device 300 ) can be maintained at a constant PH concentration required for cell culture.
- the cell culture device 400, the medium supply device 300, the cell supply source 200, the bubble removal device 100, and the pump 500 may be interconnected via a plurality of connection pipes 810,820,830,840,850. have.
- the seeding medium stored in the cell supply source 200 or the culture medium stored in the medium supply device 300 is selectively supplied to the bubble removal device 100 through opening and closing of at least one valve 600 , 700 .
- the culture medium stored in the medium supply device 300 is changed to the opening/closing state of the at least one valve 600 and 700 and the pump 500 is driven through the medium supply device 300, the bubble removal device ( 100) and the cell culture apparatus 400 may be sequentially cycled.
- the culture medium may be supplied to the bubble removal device 100 from the medium supply device 300 , and the culture medium introduced into the bubble removal device 100 is the bubble removal device 100 . ) may be supplied to the cell culture device 400 side after the air bubbles are removed. In addition, the culture medium moved to the cell culture device 400 is recovered to the medium supply device 300 and restored to a constant PH concentration required for cell culture, it can be supplied to the bubble removal device 100 again. .
- the seeding medium stored in the cell supply source 200 may be supplied to the bubble removal device 100 through opening and closing of the at least one valve 600 , 700 .
- the seeding medium may be moved to the bubble removal device 100 by the injection pressure provided from the cell supply source 200 and then supplied to the cell culture device 400 side.
- the seeding medium may be supplied to the cell culture device 400 side in a state in which the bubbles are removed from the bubble removal device 100 .
- the cells included in the seeding medium can be smoothly attached to the side of the support accommodated in the cell culture apparatus 400, and the seeding medium moved to the cell culture apparatus 400 is the cell culture apparatus ( 400) may stay in the cell culture apparatus 400 without moving to the medium supply apparatus 300 side.
- the cells included in the seeding medium supplied from the bubble removal apparatus 100 to the cell culture apparatus 400 can be smoothly attached to the surface of the support in the cell culture apparatus 400 .
- the above-described bubble removal device 100 has been illustrated and described as being applied to a circulation system in which the medium is circulated, but the present invention is not limited thereto and may be applied to a stationary cell culture system in which the medium is not circulated.
- the above-described bubble removal apparatus 100 may be applied to a process of seeding cells or a process of first injecting a medium in a stationary cell culture system.
- the cells cultured in the cell culture apparatus 300 can be utilized as cells for producing a large amount of artificial nano-vesicles. That is, the cells cultured in the cell culture apparatus 300 may be converted into artificial nanovesicles through cell disruption.
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Abstract
Description
Claims (13)
- 배지로부터 기포를 제거하기 위한 장치로서,상기 배지를 일정량 저장하기 위한 내부공간과, 외부로부터 상기 내부공간으로 상기 배지가 유입되는 유입구 및 상기 내부공간에서 기포가 제거된 상태의 배지를 외부로 배출되는 유출구를 포함하는 하우징; 및상기 하우징을 바닥면으로부터 일정높이 이격된 상태로 유지할 수 있도록 상기 하우징에 결합되는 지지부;를 포함하고,상기 내부공간은 상기 배지가 일정량 채워지는 제1공간과 상기 제1공간의 상부에 위치하면서 상기 배지가 채워지지 않는 제2공간을 포함하며,상기 제1공간 및 제2공간은 외부로부터 상기 유입구를 통해 상기 내부공간으로 유입된 상기 배지의 수위를 통해 구분되는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 유입구는 상기 유출구보다 상대적으로 높은 위치에 위치하도록 상기 하우징에 구비되고,상기 유출구는 상기 제1공간과 연통되는 위치에 위치하도록 상기 하우징에 구비되며,상기 유입구는 상기 제2공간과 연통되는 위치에 위치하도록 상기 하우징에 구비되는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 제2공간은 상기 유입구를 통해 상기 내부공간 측으로 유입되는 배지가 상기 제1공간으로 낙하하면서 기포가 분리되는 기포분리공간인 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 유출구는 상기 내부공간의 바닥면에 위치하도록 상기 하우징에 구비되는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 배지로부터 기포를 제거하기 위한 기포제거장치는,상기 제2공간과 연통되도록 상기 하우징에 구비되는 벤트구를 더 포함하는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제5항에 있어서,상기 배지로부터 기포를 제거하기 위한 기포제거장치는,상기 내부공간의 내부압력을 조절하여 상기 내부공간에 저장되는 상기 배지의 양을 조절할 수 있도록 상기 벤트구에 결합되는 압력조절수단을 더 포함하는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 하우징은, 상기 내부공간을 갖는 함체형상의 하우징몸체와, 상기 내부공간의 개방된 상부를 덮을 수 있도록 상기 하우징몸체와 착탈가능하게 결합되는 덮개부재를 포함하는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 배지로부터 기포를 제거하기 위한 기포제거장치는,상기 내부공간에 일정량 저장된 상기 배지의 상태를 감지하기 위한 센서를 포함하는 센싱부를 더 포함하는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제8항에 있어서,상기 센서는,상기 배지의 PH를 감지하기 위한 PH센서 및 상기 배지의 온도를 측정하기 위한 온도센서 중 적어도 어느 하나를 포함하는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제8항에 있어서,상기 센서는 일단부를 포함하는 일부길이가 상기 제1공간에 채워진 배지에 잠기도록 배치되는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제8항에 있어서,상기 센싱부는 상기 하우징의 일측에 착탈가능하게 결합되는 배지로부터 기포를 제거하기 위한 기포제거장치.
- 제1항에 있어서,상기 배지는 배양될 세포가 포함된 씨딩용 배지이거나 영양분을 포함하는 배양용 배지인 배지로부터 기포를 제거하기 위한 기포제거장치.
- 배양될 세포가 부착되는 적어도 하나의 지지체가 설치되는 세포배양장치;상기 세포의 배양을 위한 배양용 배지가 내부에 일정량 저장되는 배지공급장치;상기 지지체에 부착될 세포를 포함하는 씨딩용 배지가 일정량 저장되는 세포공급원;상기 배지공급장치로부터 유입된 상기 배양용 배지 또는 상기 세포공급원으로부터 유입된 상기 씨딩용 배지로부터 기포를 제거한 후 상기 세포배양장치 측으로 공급하기 위한 기포제거장치; 및상기 배양용 배지를 순환시키기 위한 펌프;를 포함하고,상기 기포제거장치는 청구항 제1항 내지 제12항 중 어느 한 항에 기재된 배지로부터 기포를 제거하기 위한 기포제거장치인 배지순환식 세포배양 시스템.
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KR20030061062A (ko) * | 2002-01-10 | 2003-07-18 | 설동근 | 가압식 세포 배양기 |
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