WO2015152412A1 - リゾリン脂質受容体を活性化する物質を含有する薬剤送達促進剤 - Google Patents
リゾリン脂質受容体を活性化する物質を含有する薬剤送達促進剤 Download PDFInfo
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Definitions
- the present invention relates to a drug delivery promoter containing a substance that activates a lysophospholipid receptor and a pharmaceutical composition containing a substance that activates a lysophospholipid receptor.
- Angiogenesis is involved in many diseases such as tumors, diabetic retinopathy, age-related macular degeneration, chronic inflammatory diseases such as rheumatoid arthritis, acute inflammation such as infections, vascular malformations, and arteriosclerosis.
- Diseases in which angiogenesis is related to pathogenesis are collectively referred to as vascular disease.
- vascular disease Diseases in which angiogenesis is related to pathogenesis are collectively referred to as vascular disease.
- angiogenesis or vasculogenesis In the fetal period, new blood vessels are formed from the absence of any blood vessels. This process is called angiogenesis or vasculogenesis.
- angiogenesis the process in which new blood vessels are formed from existing blood vessels.
- Angiogenesis is involved in angiogenesis of various pathologies.
- VEGF vascular endothelial growth factor
- vascular endothelial growth factor plays a leading role in all angiogenesis such as physiological angiogenesis from fetal period to growth period and pathological angiogenesis in pathogenesis.
- VEGF strongly induces the proliferation of vascular endothelial cells.
- VEGF phosphorylates VE-cadherin involved in adhesion between vascular endothelial cells via Src at the start of angiogenesis, induces intracellular translocation, and causes loosening between vascular endothelial cells.
- the lumen of normal blood vessels is structurally stabilized by adhesion between vascular endothelial cells and wall cells.
- the vascular endothelial cells are in close contact with each other by various adhesion factors such as VE-cadherin, claudin 5, integrin, and connexin, and are controlled so that substances and cells do not leak out of the blood vessel easily.
- Adhesive bands are formed between vascular endothelial cells and wall cells, and vascular permeability is controlled through molecular exchange between vascular endothelial cells and wall cells.
- the left and right blood vessels usually exhibit parallel running characteristics.
- vascular endothelial cells themselves also have an abnormal morphology, and the wall cells to be lined are very sparse in the center of the tumor, the adhesion to the vascular endothelial cells is weak, and the wall cell lining is missing in many areas. Many of these abnormalities are due to excessive VEGF secretion in the tumor.
- angiogenesis inhibitors The main target of angiogenesis inhibitors is VEGF or its receptor, and VEGF neutralizing antibody, soluble VEGF receptor, and multiple phosphorylation inhibitors of VEGF receptor are clinically applied.
- angiogenesis inhibitors were expected to suppress angiogenesis in the tumor, cut off the supply of oxygen and nutrients, and suppress tumor growth. This is based on the concept of tumor angiogenesis proposed by Dr. J. Folkman in the 1970s (Non-patent Document 1). In preclinical studies using mice, a significant anti-tumor effect was observed with the angiogenesis inhibitor alone, but in humans, the anti-tumor effect with the angiogenesis inhibitor alone was limited. It has been found that by using an angiogenesis inhibitor and an anticancer agent in combination, an inhibitory effect on tumor growth is observed as compared to the anticancer agent alone (Non-patent Document 2).
- Non-Patent Document 3 Non-Patent Document 3
- the intracellular signal of the VEGF receptor derived from VEGF is blocked, dissociation between vascular endothelial cells is suppressed, and the vascular permeability promoted by VEGF excess returns to normal.
- the vascular pressure is higher than the tumor tissue pressure, and the permeability of the anticancer drug from the blood vessel to the tumor is restored.
- the effect of the cancer drug is remarkably enhanced.
- angiogenesis inhibitors suppress the survival of vascular endothelial cells and induce cell death of vascular endothelial cells and vascular wall cells interacting with them to promote ischemic conditions in tumors has been. It has been pointed out that hypoxia in the tumor may induce malignant transformation of cancer cells and promote cancer invasion and metastasis. In addition, it has been reported that angiogenesis inhibitors also damage normal blood vessels and cause serious side effects such as increased blood pressure, pulmonary hemorrhage, and renal damage.
- the present invention finds a substance that normalizes blood vessel permeability at a diseased site without affecting normal blood vessels in a disease with angiogenesis abnormality such as solid cancer, and provides a novel use of the substance Is an issue.
- a drug delivery accelerator for promoting the delivery of a therapeutic agent for a disease associated with an angiogenesis disorder to a disease site comprising a substance that activates a lysophospholipid receptor as an active ingredient, and an angiogenesis abnormality
- the drug delivery promoting agent according to [1], wherein the substance that activates the lysophospholipid receptor is lysophospholipid, a precursor thereof, or a derivative thereof.
- [3] Diseases accompanied with angiogenesis are solid cancer, age-related macular degeneration, rheumatoid arthritis, psoriasis, scleroderma, systemic lupus erythematosus, vasculitis syndrome, vascular Behcet, diabetic retinopathy, diabetic nephropathy,
- the drug delivery promoting agent according to the above [3], wherein the disease accompanied by angiogenesis abnormality is solid cancer.
- the lysophospholipid is selected from lysophosphatidic acid, lysophosphatidylserine, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylinositol, lysophosphatidylglycerol, sphingosine-1-phosphate, sphingosylphosphorylcholine and platelet activating factor (PAF)
- the drug delivery promoter according to any one of [1] to [4], wherein the drug delivery promoter is one or more kinds.
- the drug delivery accelerator according to [5] wherein the lysophospholipid is lysophosphatidic acid.
- a pharmaceutical composition for treating a disease associated with abnormal angiogenesis comprising a substance that activates a lysophospholipid receptor as an active ingredient.
- the pharmaceutical composition according to the above [8] or [9] which is used in combination with another drug for treating a disease accompanied by angiogenesis abnormality.
- the pharmaceutical composition according to the above [10], wherein the other drug for treating a disease accompanied by angiogenesis abnormality is a cancer therapeutic drug.
- the disease accompanied by angiogenesis is solid cancer, age-related macular degeneration, rheumatoid arthritis, psoriasis, scleroderma, systemic lupus erythematosus, vasculitis syndrome, vascular Behcet, diabetic retinopathy, diabetic nephropathy,
- the pharmaceutical composition according to the above [13], wherein the disease accompanied by angiogenesis abnormality is solid cancer.
- the lysophospholipid is selected from lysophosphatidic acid, lysophosphatidylserine, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylinositol, lysophosphatidylglycerol, sphingosine-1-phosphate, sphingosylphosphorylcholine and platelet activating factor (PAF)
- PAF platelet activating factor
- the pharmaceutical composition according to any one of the above [7] to [14], which is one kind or two or more kinds.
- the pharmaceutical composition according to the above [15], wherein the lysophospholipid is lysophosphatidic acid.
- a screening method for a therapeutic agent for a disease associated with an angiogenesis abnormality comprising selecting a substance that activates a lysophospholipid receptor specifically expressed in vascular endothelial cells at a disease site Method.
- the drug delivery promoter containing a substance that activates the lysophospholipid receptor of the present invention as an active ingredient normalizes the permeability of blood vessels at a disease site accompanied by abnormal angiogenesis without affecting normal blood vessels, Delivery of drugs used in combination to a diseased site accompanied by an angiogenesis abnormality can be promoted, and a high therapeutic effect can be obtained with a small amount of therapeutic agent.
- the pharmaceutical composition containing a substance that activates the lysophospholipid receptor of the present invention as an active ingredient can be used alone for the treatment of diseases accompanied by abnormal angiogenesis.
- the pharmaceutical composition of the present invention is used for the treatment of solid cancer, it suppresses tumor growth, suppresses cancer cell metastasis, and induces tumor immunity without inducing malignant transformation of cancer cells. There is an effect that it can be enhanced.
- FIG. 1 It is a figure which shows the result of having administered lysophosphatidic acid (LPA) to the tumor-bearing mouse
- LPA lysophosphatidic acid
- FIG. 1 It is a figure which shows the result observed with the focus laser microscope. It is a figure which shows the result of having administered the lysophosphatidic acid (LPA) to the tumor-bearing mouse
- LPA lysophosphatidic acid
- FIG. 3 shows the results of lysophosphatidic acid (LPA) and FITC-labeled dextran (70 kDa or 2000 kDa) having different molecular weights administered to tumor-bearing mice with LLC cells, and drug delivery from tumor blood vessels to tumor tissues observed with a confocal laser microscope.
- FIG. It is a figure which shows the result of having examined the combined use effect of lysophosphatidic acid (LPA) and 5-FU in the tumor-bearing mouse
- LPA lysophosphatidic acid
- FIG. 7 is a view showing the results of studying the combined effect of lysophosphatidic acid (LPA) and 5-FU in tumor-bearing mice of B16-BL6 cells, (A) shows the time course of tumor volume in each group; B) is a diagram showing the appearance of a representative mouse in each group. It is a figure which shows the result of having examined the combined use effect of lysophosphatidic acid (LPA) and 5-FU in the tumor-bearing mouse
- LPA lysophosphatidic acid
- 5-FU in the tumor-bearing mice
- FIG. 2 is a view showing the results of lysophosphatidic acid (LPA) administration to tumor-bearing mice of LLC cells and observing the expression of VE-cadherin in vascular endothelial cells in the tumor
- FIG. 1 shows a Z-stack image taken along the oblique white line portion shown in (A), and VE-cadherin is obtained. It is a figure which shows the result of having analyzed the expression level (fluorescence intensity). It is a figure which shows the result of having added VPC31144S of LPA or the LPA derivative to the cultured endothelial cell, and analyzing the adhesive strength (barrier function) between endothelial cells. It is a figure which shows the result of having administered the lysophosphatidic acid (LPA) to the tumor-bearing mouse
- LPA lysophosphatidic acid
- FIG. 6 is a diagram showing the results of analyzing the expression level of LPA receptors (LPAR) 1-6 in vascular endothelial cells of tumor tissue collected from tumor-bearing mice with LLC cells using real-time PCR.
- FIG. 3 is a diagram showing the results of analyzing the expression level of LPA receptors (LPAR) 1-6 in MS-1 cells (mouse pancreatic vascular endothelial cell line) using real-time PCR. It is a figure which shows the result of having culture
- Lysophospholipid is a phospholipid having one acyl group. Lysophospholipids are roughly classified into classes having a glycerol skeleton and a sphingosine skeleton, and there are a large number of molecular species depending on combinations of polar groups and acyl groups bonded to each. Lysophospholipids are known to be lipid mediators that exhibit various biological activities by binding to specific receptors. However, there are still many unclear points about the physiological function of lysophospholipids in vivo, and the effect on blood vessels of diseases associated with abnormal angiogenesis has not been known at all.
- lysophosphatidic acid which is a kind of lysophospholipid
- mice in which tumor cells were transplanted subcutaneously meandering and disordered branching were observed before administration. It was found that the tumor blood vessels changed to a network structure similar to that of normal tissues. Regarding the structure of the lumen of the tumor blood vessel, it was found that the structure was distorted before lysophosphatidic acid administration, but changed to a smooth structure after administration. Furthermore, regarding tumor blood vessel permeability, it was found that lysophosphatidic acid improves the excessive enhancement state and induces vascular permeability normally.
- LPA lysophosphatidic acid
- the present inventors lysophosphatidic acid, in diseases accompanied by angiogenesis abnormalities such as solid cancer, the action of inducing the network construction of blood vessels to normalize the network structure, the action of smoothing the blood vessel lumen, It has been found that it has an effect of normalizing vascular permeability.
- the present inventors conducted experiments using lower limb ischemia model mice, which are pathological models of Buerger's disease and chronic obstructive arteriosclerosis, and age-related macular degeneration model mice. Furthermore, it was found that lysophosphatidic acid normalizes an increased state of vascular permeability and improves the disease state.
- lysophosphatidic acid has an action to normalize abnormal blood vessels in diseases accompanied by abnormal angiogenesis. Therefore, lysophospholipid is considered to be useful as an active ingredient of a vascular normalizing agent in diseases accompanied by angiogenesis abnormalities.
- LPAR LPAR receptors
- LPAR1 to LPAR6 six types of LPAR receptors
- the expression of LPAR1 to 3 is considered to be high in cancer cells, and the growth of cancer cells is induced by lysophosphatidic acid in in vitro culture.
- the present inventors analyzed LPAR expressed in vascular endothelial cells of tumor tissue using mice, and found that LPAR1, LPAR4 and LPAR6 are expressed. Furthermore, it was found that adhesion between cells is distorted in vascular endothelial cells in which LPAR4 is knocked down. That is, it was found that the normalization of tumor blood vessels is at least via LPAR4.
- LPAR that is specifically expressed in tumor vascular endothelial cells is specifically activated without activating LPAR1 to 3 that are highly expressed in cancer cells, proliferation of cancer cells and enhancement of motility are promoted. Cancer can be treated without concern. That is, it is considered that a receptor agonist capable of specifically activating LPAR4 or LPAR6 is useful as an active ingredient of a vascular normalizing agent, like lysophospholipid. Furthermore, although LPA receptors 1-6 have been identified at present, LPA receptor agonists that can induce vascular normalization, including receptors not yet identified, are useful as active ingredients for vascular normalizing agents. It is thought that.
- the present invention provides a drug delivery enhancer for facilitating delivery of a therapeutic agent for a disease associated with abnormal angiogenesis to a disease site.
- the drug delivery promoter of the present invention contains a substance that activates a lysophospholipid receptor as an active ingredient, and is characterized by being used in combination with a therapeutic agent for diseases associated with abnormal angiogenesis. It is known that in a diseased site accompanied with an angiogenesis abnormality, blood flow is poor and vascular permeability is excessively increased. For example, in solid cancer, it is known that the tumor interstitial pressure is increased, there is no difference in osmotic pressure in the tumor stroma and in the blood vessels, and it is difficult to transfer substances from the blood vessel lumen into the tumor tissue. It has been. Since the drug delivery promoter of the present invention can normalize abnormal blood vessels at diseased sites with angiogenesis abnormalities and normalize vascular permeability, the delivery efficiency of the drugs used in combination to the diseased sites is remarkably improved. Can be increased.
- the substance that activates the lysophospholipid receptor is not limited to lysophospholipids, and derivatives of lysophospholipids, precursors of lysophospholipids or derivatives thereof can also be suitably used as active ingredients.
- lysophospholipid receptor agonists other than these eg, low molecular weight compounds, nucleic acids, peptides, proteins, antibodies, etc.
- Known lysophospholipid receptor agonists include, for example, the LPA4 receptor agonist described in Wong et al. (AssayAsDrug Dev Technol. 2010 Aug; 8 (4): 459-70. Doi: 10.1089 / adt.2009.0261.) Is mentioned.
- it is a lysophospholipid or a precursor thereof, or a derivative thereof.
- lysophospholipid used in the drug delivery accelerator of the present invention examples include lysophosphatidic acid (LPA), lysophosphatidylserine (LPS), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI), Examples include, but are not limited to, lysophosphatidylglycerol (LPG), sphingosine-1-phosphate (S1P), sphingosylphosphorylcholine (SPC), platelet activating factor (PAF), and other lysophospholipids. Can be suitably used.
- LPA lysophosphatidic acid
- LPS lysophosphatidylserine
- LPC lysophosphatidylcholine
- LPE lysophosphatidylethanolamine
- LPI lysophosphatidylinositol
- lysophosphatidic acid Preferred are lysophosphatidic acid, lysophosphatidylcholine and sphingosine-1-phosphate, more preferably lysophosphatidic acid.
- One kind of lysophospholipid may be used in the drug delivery accelerator of the present invention, or two or more kinds may be used in combination.
- the acyl group of the lysophospholipid is not particularly limited, but an acyl group having 16 to 22 carbon atoms and having an unsaturation degree of 0 to 6 is preferable. More preferably, the carbon number: unsaturation is 16: 1, 18: 1, 18: 2, 18: 3, 20: 1, 20: 2, 20: 3, 20: 4, 20: 5, 22: 1. 22: 2, 22: 3, 22: 4, 22: 5, 22: 6.
- the lysophospholipid may be either a 1-acyl lysophospholipid or a 2-acyl lysophospholipid. A 1-acyl lysophospholipid is preferable.
- lysophospholipid examples include phosphatidic acid, phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, sphingolipid and the like. It is well known to those skilled in the art that these phospholipids are metabolized in vivo to produce lysophospholipids (for example, E. J. Goetzl, S. An, FASEB J. 12, 1589 (1998), Xie Y , Meier KE. Cell Signal. 2004 Sep; 16 (9): 975-81).
- lysophospholipid modified with a water-soluble polymer such as lysophospholipid modified with polyethylene glycol (PEG) derivative (PEGylated lysophospholipid), polyglycerin for the purpose of improving stability in blood, arbitrary lysophospholipid
- PEG polyethylene glycol
- examples thereof include lysophospholipid modified with a substituent.
- the derivative of lysophospholipid precursor include a precursor modified with a PEG derivative, a precursor modified with a water-soluble polymer, a precursor modified with an arbitrary substituent, and the like.
- lysophospholipid or a precursor thereof, or a derivative thereof may form a salt, and a physiologically acceptable salt is preferable as the salt.
- Physiologically acceptable salts include, for example, hydrochloric acid, sulfuric acid, phosphoric acid, lactic acid, tartaric acid, maleic acid, fumaric acid, oxalic acid, malic acid, citric acid, oleic acid, palmitic acid, nitric acid, phosphoric acid, trifluoro Salts with acids such as acetic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid; salts with alkali or alkaline earth metals such as sodium, potassium, calcium, or aluminum hydroxides or carbonates And salts with triethylamine, benzylamine, diethanolamine, t-butylamine, dicyclohexylamine, arginine and the like.
- the lysophospholipid used in the present invention or a precursor thereof, or a derivative thereof is, for example, known as (1) chemically synthesized; (2) purified from a biological sample; (3) enzymatically synthesized; Can be obtained by the method. Moreover, a commercial item can be purchased and used.
- chemical synthesis for example, Comprehensive Organic Transformations: A Guide to Functional Group Preparations, Second Edition (Richard C. Lalock, John Wiley and Sons Inc, 1999).
- purifying from a biological sample for example, it can be produced by subjecting a fraction obtained by a method such as gel filtration to a purification method such as silica gel column chromatography or reverse phase column chromatography.
- a purification method such as silica gel column chromatography or reverse phase column chromatography.
- myeloperoxidase, oxidase, 12 / 15-lipoxygenase, P450 metabolic enzyme, etc. can be used.
- the lysophospholipid receptor activated by the active ingredient is not particularly limited, and a known lysophospholipid receptor and a lysophospholipid receptor found in the future can be targeted. Among these, a lysophospholipid receptor expressed in vascular endothelial cells is preferable, and a lysophospholipid receptor specifically expressed in vascular endothelial cells is more preferable.
- the present inventors have found that LPAR4 and LPAR6 are specifically expressed in vascular endothelial cells of mouse tumor tissue (see Example 10), and that LPAR4 is particularly preferred. Confirmed (see Example 11). Therefore, as the lysophospholipid receptor activated by the active ingredient, human LPAR corresponding to mouse LPAR4 and LPAR6 is preferable.
- Diseases with angiogenesis abnormalities include diseases where blood vessels meandering to the affected area and blood vessels with disordered branches are formed, diseases where blood vessels having a distorted lumen structure are formed in the affected area, and increased vascular permeability in the affected area Diseases in which damaged blood vessels are formed are included.
- Examples of the disease accompanied by angiogenesis abnormality in which drug delivery is promoted by the drug delivery promoter of the present invention include solid cancer, age-related macular degeneration, rheumatoid arthritis, psoriasis, scleroderma, systemic lupus erythematosus, vasculitis Syndrome, vascular Behcet, diabetic retinopathy, diabetic nephropathy, arteriosclerosis, chronic obstructive arteriosclerosis, Buerger's disease, pulmonary fibrosis, cerebral infarction, severe infection, heart failure, etc. Not.
- Solid cancer blood vessels with meandering and disordered branching are formed inside, the structure of the blood vessel lumen is distorted, and blood vessel permeability is excessively increased.
- Solid cancer is not particularly limited, and for example, lung cancer, colon cancer, prostate cancer, breast cancer, pancreatic cancer, esophageal cancer, stomach cancer, liver cancer, biliary tract cancer, spleen cancer, kidney cancer, bladder Cancer, uterine cancer, ovarian cancer, testicular cancer, thyroid cancer, brain tumor and the like.
- solid cancers include cancerous blood cells that form tumors.
- Age-related macular degeneration is a disease in which choroidal neovascularization with enhanced permeability is formed.
- Rheumatoid arthritis is a disease in which new blood vessels with increased permeability are formed due to inflammation in the synovium in the joint. Joint fluid accumulates in the joint cavity, causing joint deformation and pain.
- Psoriasis is an inflammatory disease in which blood vessels having a distorted structure meandering in the stratum corneum of the skin are constructed, and inflammation and keratin thickening are involved.
- Scleroderma is a collagen disease and is a disease in which the formation of new blood vessels with increased permeability is induced by inflammation and the skin is always in an inflammatory state.
- systemic lupus erythematosus, vasculitis syndrome, and vascular Behcet are diseases in which the formation of new blood vessels with increased permeability is induced by inflammation.
- Diabetic retinopathy is a disease in which capillaries of the retina are damaged and become brittle due to persistent hyperglycemia, and some of them become lumped to form abnormally structured blood vessels.
- a new blood vessel is formed when a blood clot is blocked due to a thrombus, but this new blood vessel is fragile and has increased permeability, resulting in bleeding and blindness.
- diabetic nephropathy glomerular capillaries are damaged by an increase in blood glucose level, and the formation of new blood vessels with increased permeability is induced.
- Arteriosclerosis is a disease in which cholesterol or the like accumulates in blood vessels, a sclerotic nest is formed, the blood vessel lumen becomes narrow, and blood flow decreases. In this atherosclerotic lesion, distorted blood vessels are grown, which contributes to an increase in the cured lesion.
- chronic obstructive arteriosclerosis large blood vessels in the extremities are chronically constricted to reduce blood flow and become hypoxic, leading to the formation of new blood vessels with increased permeability, resulting in edema and inflammation.
- Buerger's disease is also called Buerger's disease and obstructive thromboangiitis, and the cause is not clear yet.
- Pulmonary fibrosis is a disease in which the inflammatory site of the lung becomes fibrotic due to interstitial pneumonia, but in the acute phase, inflammatory cell infiltration into the lung interstitium causes an increase in the permeability of new blood vessels.
- Cerebral infarction is a disease in which blood flow is reduced due to cerebral blood vessel embolization, leading to neuronal cell death.
- VEGF vascular endothelial growth factor
- heart failure is caused by hypoxia with reduced blood flow throughout the body, forming new blood vessels with increased permeability and increasing permeability of existing blood vessels, pleural effusion, hepatic congestion, gastrointestinal edema It is a disease that causes pulmonary congestion.
- the therapeutic agents for the diseases exemplified above can be suitably used.
- cancer therapeutic drugs anti-inflammatory drugs, neuropsychiatric drugs, sensory organ drugs, cardiovascular drugs, respiratory drugs, digestive drugs, endocrine metabolic drugs, kidney / urological drugs , Vitamins / nutrition / infusion solutions / electrolyte preparations, blood drugs / blood preparations, immunosuppressants, analgesics, antiallergic agents, antibiotics, antibacterial agents, antiviral agents and the like.
- the use of a combination of a drug delivery accelerator and a therapeutic agent for a disease associated with an angiogenesis disorder means that the application time of the drug delivery accelerator of the present invention and the application time of the therapeutic drug overlap. It does not require administration at the same time.
- the present invention provides a pharmaceutical composition for treating a disease associated with an angiogenesis abnormality, which contains a substance that activates a lysophospholipid receptor as an active ingredient.
- the drug delivery promoter of the present invention is preferably implemented in the form of a pharmaceutical product.
- the pharmaceutical composition of the present invention may be used alone or in combination with other drugs for treating diseases associated with abnormal angiogenesis.
- Substances that activate lysophospholipid receptors are not limited to lysophospholipids, and lysophospholipid derivatives, lysophospholipid precursors or derivatives thereof can also be suitably used as active ingredients.
- lysophospholipid receptor agonists other than these (eg, low molecular weight compounds, nucleic acids, peptides, proteins, antibodies, etc.) can also be suitably used as active ingredients.
- Known lysophospholipid receptor agonists include, for example, LPA4 receptor agonists described in Wong et al. (Assay Drug Dev Technol. 2010 Aug; 8 (4): 459-70. Doi: 10.1089 / adt.2009.0261.) Is mentioned.
- it is a lysophospholipid or a precursor thereof, or a derivative thereof.
- a substance that activates a lysophospholipid receptor, which is an active ingredient of the pharmaceutical composition of the present invention, is one selected from a tumor growth inhibitory action, a cancer metastasis inhibitory action, a tumor immunity enhancing action, and a vascular permeability enhancement inhibiting action Since it has the above effect
- Diseases with angiogenesis abnormalities to be treated are as described above, for example, solid cancer, age-related macular degeneration, rheumatoid arthritis, psoriasis, scleroderma, systemic lupus erythematosus, vasculitis syndrome, vascular Behcet, diabetic retina , Diabetic nephropathy, arteriosclerosis, chronic obstructive arteriosclerosis, Buerger's disease, pulmonary fibrosis, cerebral infarction, severe infection, heart failure and the like.
- the pharmaceutical composition of the present invention comprises a substance that activates a lysophospholipid receptor as an active ingredient, and is pharmaceutically acceptable according to a method known as a method for producing a pharmaceutical preparation (for example, a method described in the Japanese Pharmacopoeia).
- a suitable carrier or additive can be added to prepare a preparation.
- tablets including sugar-coated tablets, film-coated tablets, sublingual tablets, orally disintegrating tablets, buccal tablets, etc.
- pills including sugar-coated tablets, film-coated tablets, sublingual tablets, orally disintegrating tablets, buccal tablets, etc.
- pills including sugar-coated tablets, film-coated tablets, sublingual tablets, orally disintegrating tablets, buccal tablets, etc.
- pills powders, granules, capsules (including soft capsules and microcapsules) , Lozenges, syrups, solutions, emulsions, suspensions, controlled release formulations (eg, immediate release formulations, sustained release formulations, sustained release microcapsules, etc.), aerosols, film agents (eg, orally disintegrating films) , Oral mucosa adhesive film, etc.), injection (eg, subcutaneous injection, intravenous injection, intramuscular injection, intraperitoneal injection, etc.), drip, transdermal preparation, ointment, lotion, patch Oral or parenteral preparations such as sup
- the blending ratio of the carrier or additive can be appropriately set based on the range usually employed in the pharmaceutical field.
- Carriers or additives that can be blended are not particularly limited, but various carriers such as water, physiological saline, other aqueous solvents, aqueous or oily bases; excipients, binders, pH adjusters, disintegrants, absorption enhancers Various additives such as an agent, a lubricant, a coloring agent, a corrigent, and a fragrance are included.
- Additives that can be mixed into tablets, capsules and the like include binders such as gelatin, corn starch, tragacanth and gum arabic, excipients such as crystalline cellulose, corn starch, gelatin, alginic acid and the like A swelling agent, a lubricant such as magnesium stearate, a sweetening agent such as sucrose, lactose or saccharin, a flavoring agent such as peppermint, red oil, or cherry are used.
- a liquid carrier such as fats and oils can be further contained in the above type of material.
- a sterile composition for injection can be prepared according to a usual formulation procedure (for example, an active ingredient is dissolved or suspended in a solvent such as water for injection or natural vegetable oil).
- aqueous solutions for injection include physiological saline, isotonic solutions containing glucose and other adjuvants (eg, D-sorbitol, D-mannitol, sodium chloride, etc.), and suitable solubilizing agents such as You may use together with alcohol (ethanol etc.), polyalcohol (propylene glycol, polyethylene glycol, etc.), nonionic surfactants (polysorbate 80 TM , HCO-50, etc.).
- oily liquid for example, sesame oil, soybean oil and the like are used, and they may be used in combination with solubilizing agents such as benzyl benzoate and benzyl alcohol.
- solubilizing agents such as benzyl benzoate and benzyl alcohol.
- buffers eg phosphate buffer, sodium acetate buffer etc.
- soothing agents eg benzalkonium chloride, procaine hydrochloride etc.
- stabilizers eg human serum albumin, polyethylene glycol etc.
- preservatives eg For example, you may mix
- antioxidant etc.
- the pharmaceutical composition of the present invention can be used in humans and other mammals (for example, rats, mice, rabbits, Sheep, pigs, cows, cats, dogs, monkeys, etc.) have low toxicity and can be safely administered.
- the pharmaceutical composition of the present invention varies depending on the dosage form, administration method, carrier and the like, but when the active ingredient is lysophospholipid or a derivative thereof, the active ingredient is usually 0.01 to 100% (w / w) based on the total amount of the preparation. By adding w), preferably at a ratio of 0.1 to 95% (w / w), it can be produced according to a conventional method.
- the dose varies depending on the administration subject, symptom, administration route and the like, but in the case of oral administration, for example, generally about 0.01 to 1000 mg, preferably about 0.1, per day for a human body weight of about 60 kg. 1 to 100 mg, more preferably about 0.5 to 500 mg.
- the single dose varies depending on the patient's condition, symptoms, administration method, etc. For example, in the case of injection, it is usually about 0.01-100 mg per kg body weight, preferably about 0.01 ⁇ 50 mg, more preferably about 0.01-20 mg is administered intravenously.
- the total daily dose may be a single dose or divided doses.
- the pharmaceutical composition of the present invention is preferably used in combination with other drugs for treating diseases associated with angiogenesis abnormalities.
- Other drugs for treating diseases associated with abnormal angiogenesis are not particularly limited, and for example, known drugs conventionally used for the treatment of diseases associated with abnormal angiogenesis exemplified above can be suitably used.
- the pharmaceutical composition of the present invention in combination with other drugs, it is possible to promote the delivery of other drugs to a diseased site accompanied by an angiogenesis abnormality, so that the amount of the drug used can be reduced. . As a result, side effects caused by other drugs can be reduced. In addition, the reduction in the amount of drug used meets social demands such as medical cost reduction.
- cancer therapeutic agent used in combination with the pharmaceutical composition of the present invention is not particularly limited, for example, a chemotherapeutic agent, an immunotherapeutic agent or a hormone therapeutic agent is preferable. These cancer therapeutic agents may be liposome preparations. These cancer therapeutic agents may be nucleic acid drugs or antibody drugs. The chemotherapeutic agent is not particularly limited.
- nitrogen mustard nitrogen mustard hydrochloride-N-oxide, chlorambutyl, cyclophosphamide, ifosfamide, thiotepa, carbocon, improsulfan tosylate, busulfan, nimustine hydrochloride, mitoblonitol , Melphalan, dacarbazine, ranimustine, sodium estramustine phosphate, triethylenemelamine, carmustine, lomustine, streptozocin, piprobroman, etoglucid, carboplatin, cisplatin, miboplatin, nedaplatin, oxaliplatin, altretamine, ambaspine hydrochloride Fotemustine, prednimustine, pumitepa, ribomustine, temozolomide, treosulfan, tropho Alkylating agents such as famide, dinostatin stimamarer, adzelesin,
- Examples include parbum, levamisole, polysaccharide K, procodazole, ipilimumab, nivolumab, ramcilmab, ofatumumab, panitumumab, pembrolizumab, obinuzumab, trastuzumab emtansine, tocilizumab, betizumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab, trastuzumab,
- Hormonal therapeutic agents are not particularly limited, for example, phosfestol, diethylstilbestrol, chlorotrianicene, medroxyprogesterone acetate, megestrol acetate, chlormadinone acetate, cyproterone acetate, danazol, allylestrenol, gestrinone, Mepaltricin, raloxifene, olmeroxifene, levormeloxifene, antiestrogens (eg tamoxifen citrate, toremifene citrate, etc.), pill formulations, mepithiostan, testrolactone, aminoglutethimide, LH-RH agonists (eg goserelin acetate, Buserelin, leuprorelin, etc.), droloxifene, epithiostanol, ethinyl estradiol sulfonate, aromatase inhibitor (eg, fadroz
- the pharmaceutical composition of the present invention When used in combination with other drugs for treating diseases accompanied by angiogenesis abnormalities, these may be administered simultaneously to the subject of administration, or administered at a time lag. Good.
- “used in combination” means that the application time of the drug delivery accelerator of the present invention and the application time of the therapeutic agent overlap, and do not require simultaneous administration.
- the dosage of other drugs for the treatment of diseases accompanied by abnormal angiogenesis may be in accordance with the clinically used dosage, and the administration subject, the age and weight of the administration subject, symptoms, administration time, dosage form, administration It can select suitably by a method, a combination, etc.
- the present invention provides a screening method for a therapeutic agent for a disease associated with abnormal angiogenesis.
- the screening method of the present invention is characterized by selecting a substance (lysophospholipid receptor agonist) that activates a lysophospholipid receptor specifically expressed in vascular endothelial cells at a disease site.
- lysophospholipid receptor examples include lysophosphatidic acid receptor (LPAR), lysophosphatidylserine receptor (LPSR), lysophosphatidylcholine receptor (LPCR), lysophosphatidylethanolamine receptor (LPER), lysophosphatidylinositol receptor (LPIR), lysophosphatidylglycerol receptor (LPGR), sphingosine-1-phosphate receptor (S1PR), sphingosylphosphorylcholine receptor (SPCR), platelet activating factor receptor (PAFR) and the like. It is not limited. Of these, lysophosphatidic acid receptor (LPAR) is preferable.
- the lysophospholipid receptor specifically expressed in the vascular endothelial cell at the disease site is not particularly limited, and is specifically expressed in the vascular endothelial cell at the disease site including the lysophospholipid receptor to be found in the future.
- Any lysophospholipid receptor can be preferably used as a target of the screening method of the present invention.
- the present inventors have found that LPAR4 and LPAR6 are specifically expressed in vascular endothelial cells of mouse tumor tissue (see Example 10), and that LPAR4 is particularly preferably targeted. Confirmed (see Example 11). Therefore, it is preferable to use human LPAR corresponding to mouse LPAR4 and LPAR6 in the screening method of the present invention.
- the screening method of the present invention includes, for example, a step of bringing a test substance into contact with a lysophospholipid receptor specifically expressed on a vascular endothelial cell at a disease site accompanied by an angiogenesis abnormality, and an activation state of the lysophospholipid receptor Can be carried out by a method comprising the steps of: measuring and a step of selecting a test substance to activate a lysophospholipid receptor.
- the substance selected by the screening method of the present invention is useful as an active ingredient of the drug delivery accelerator of the present invention.
- the substance selected by the screening method of the present invention is useful as an active ingredient of the pharmaceutical composition for the treatment of diseases accompanied by abnormal angiogenesis of the present invention.
- the present invention includes the following inventions.
- a method for facilitating delivery of a therapeutic agent for a disease associated with an angiogenesis disorder to a disease site comprising administering an effective amount of lysophospholipid or a precursor thereof, or a derivative thereof to a mammal, and further angiogenesis abnormality
- a method for facilitating drug delivery comprising administering an effective amount of a therapeutic agent for a disease involving a disease.
- a disease with an angiogenesis disorder characterized by administering an effective amount of a lysophospholipid or a precursor thereof, or a derivative thereof to a mammal, and further administering an effective amount of a therapeutic agent for a disease with an angiogenesis disorder Treatment methods.
- a method for treating solid cancer comprising administering an effective amount of lysophospholipid or a precursor thereof, or a derivative thereof to a mammal.
- a method for inhibiting cancer metastasis comprising administering an effective amount of lysophospholipid or a precursor thereof, or a derivative thereof to a mammal.
- a lysophospholipid or a precursor thereof, or a derivative thereof for use in inhibiting cancer metastasis Use of lysophospholipid or a precursor thereof, or a derivative thereof for producing a drug delivery enhancer for facilitating delivery of a therapeutic agent for a disease associated with an angiogenesis disorder to a disease site. Use of lysophospholipid or a precursor thereof, or a derivative thereof for the manufacture of a therapeutic agent for a disease associated with angiogenesis abnormality. Use of lysophospholipid or a precursor thereof, or a derivative thereof for producing a therapeutic agent for solid cancer. Use of lysophospholipid or a precursor thereof, or a derivative thereof for producing a cancer metastasis inhibitor.
- Example 1 Structural change of tumor blood vessel by administration of lysophosphatidic acid (hereinafter referred to as "LPA")]
- LPA lysophosphatidic acid
- Mouse tumor cell lines were transplanted subcutaneously into mice to form tumors, and then LPA was administered to observe what structural changes LPA induces on blood vessels.
- the Lewis lung cancer cell line (henceforth "LLC cell”) was used as a mouse cancer cell line. LLC cells (1 ⁇ 10 6 cells / 100 ⁇ L ⁇ PBS / mouse) were injected subcutaneously into 8-week-old C57BL / 6NCrSlc mice ( ⁇ , SLC).
- As the LPA 18: 1 LPA (Avanti POLAR LIPIDS) was used.
- a 10 mM LPA stock solution was prepared using 50% ethanol and stored at ⁇ 30 ° C. The solution was thawed at the time of use, and subdivided for 1 minute with an ultrasonic cleaner (SND), and then an LPA solution for administration was prepared at the time of use so as to be 3 mg / kg / 100 ⁇ L with PBS.
- SND ultrasonic cleaner
- a mouse having a tumor volume (major axis ⁇ minor axis ⁇ height ⁇ 0.5) of 60 to 80 mm 3 was selected and subjected to the test.
- Four groups of control group, LPA / 6 hour group, LPA / 12 hour group and LPA / 24 hour group were provided, and 3 mice per group were used.
- LPA was intraperitoneally administered to mice in the LPA group at a dose of 3 mg / kg / 100 ⁇ L. Tumors were removed from mice before LPA administration (control group) and 6, 12, and 24 hours after LPA administration.
- the excised tumor was immersed in 4% paraformaldehyde (PFA) / PBS and fixed by shaking at 4 ° C. overnight. After fixation, the tumor was washed with cold PBS (4 ° C.). Washing was performed for 6 hours and replaced with fresh PBS every 30 minutes. The tumor was then immersed in 15% sucrose / PBS and shaken at 4 ° C. for 3 hours. Next, it was immersed in 30% sucrose / PBS and shaken at 4 ° C. for 3 hours. Subsequently, the tumor was O.D. C. T. T. et al. It was embedded in a compound (Tissue-Tek) and frozen at ⁇ 80 ° C. for 3 days or more.
- PFA paraformaldehyde
- the tumor embedded with the compound was sliced into 40 ⁇ m thick sections with a cryostat (LEICA).
- the section was placed on a slide glass and air-dried with a dryer for 2 hours.
- the section was surrounded by a liquid blocker, a slide glass was set in a slide staining vat, and washed with PBS for 10 minutes at room temperature.
- C. T.A The compound was washed away. Post-fixation was performed using 4% PFA / PBS for 10 minutes at room temperature, and washing was performed using PBS for 10 minutes at room temperature.
- a blocking solution (5% normal goat serum / 1% BSA / 2% skim milk / PBS) was dropped onto the sections and blocked at room temperature for 20 minutes.
- Purified Hamster Anti-PECAM-1 (MILLIPORE: MAB1398Z), which is an anti-mouse CD31 antibody, was diluted 200-fold with a blocking solution and dropped on a section and reacted overnight at 4 ° C. . Washing with PBS containing Tween 20 (PBST) for 10 minutes was performed 5 times, and further with PBS for 10 minutes.
- PBST Tween 20
- Alexa Fluor 488 Goat Anti-Hamster IgG Jackson ImmunoResearch Laboratories
- FIG. (A) is a photograph of a specimen observed with a confocal laser microscope, in which vascular endothelial cells are stained with green fluorescence and depicted in white in the photograph.
- (B) is a figure which shows the average blood vessel length of each group computed with AngioTool blood vessel structure analysis software.
- FIG. 1 (A) the tumor blood vessels before the LPA administration had a sparse network structure and the blood vessels were discontinuous, but after 6 hours, the connected state of the blood vessels was observed. After 12 hours and 24 hours, a vascular network structure was observed as in normal tissues.
- FIG. 1 (B) it was shown as a numerical value that the length of each blood vessel was increased by administration of LPA.
- Example 2 Structural change of tumor blood vessel by administration of sphingosine-1-phosphate (hereinafter referred to as “S1P”)] Whether S1P, which is a lysophospholipid other than LPA, was used to observe whether tumor blood vessel network construction was induced as in LPA.
- S1P sphingosine-1-phosphate
- mice in the S1P group were administered S1P once daily at a dose of 0.3 mg / kg / 100 ⁇ L into the tail vein.
- PBS 100 ⁇ L was administered to the control group of mice instead of S1P.
- Tumors were removed from the mice 24 hours after the final administration, and tumor blood vessel tissue specimens were prepared in the same manner as in Example 1. Observation was performed with a confocal laser microscope (LEICA), and photography was performed.
- LEICA confocal laser microscope
- Example 3 Structural change of tumor blood vessel lumen after LPA administration
- LLC cells were transplanted subcutaneously into 8-week-old C57BL / 6NCrSlc mice (Aoi, SLC) in the same manner as in Example 1.
- LPA for administration was prepared in the same manner as in Example 1.
- mice 24 hours after LPA or PBS administration, the mice were fixed by perfusion under anesthesia with pentobarbital (Kyoritsu Pharmaceutical Co., Ltd.).
- 0.1 M phosphate buffer (pH 7.4) containing 2% formaldehyde and 2.5% glutaraldehyde was used as the fixing solution.
- the tumor was removed, immersed in the same fixing solution used for perfusion, and shaken at 4 ° C. overnight.
- a 0.1 M phosphate buffer (pH 7.4) containing 1% osmium tetroxide and 0.5% potassium ferrocyan It was dehydrated with increasing series of ethanol and replaced with t-butyl alcohol and lyophilized. After lyophilization, osmium tetroxide was deposited, and the vascular lumen surface was observed with a scanning electron microscope S-4800 manufactured by Hitachi High-Tech.
- Example 4 Improvement of drug delivery from tumor blood vessel to tumor tissue after LPA administration
- the blood vessel permeability is excessively increased in the tumor. Therefore, the tumor interstitial pressure is finally increased, the difference in osmotic pressure between the tumor stroma and the blood vessel is eliminated, and the movement of the substance from the blood vessel lumen into the tumor tissue becomes difficult. Since it was revealed that the vascular network in the tumor was densely constructed after LPA administration and the vascular lumen was also smooth, it was expected that the permeability of the drug in the tumor blood vessel after LPA administration was improved. Therefore, in order to confirm the drug permeability in the tumor after administration of LPA, the following experiment was performed.
- Example 1 Experimental method LLC cells were transplanted subcutaneously into 8-week-old C57BL / 6NCrSlc mice (Aoi, SLC) in the same manner as in Example 1.
- LPA for administration was prepared in the same manner as in Example 1.
- LPA 3 mg / kg / 100 ⁇ L
- PBS 100 ⁇ L
- doxorubicin Doxorubicin hydrochloride, Nippon Kayaku Co., Ltd.
- Doxorubicin was dissolved in physiological saline (Otsuka Pharmaceutical Co., Ltd.), diluted to 1.5 mg / kg, and then subdivided with an ultrasonic washer for 1 minute before administration.
- Doxorubicin is an anticancer agent that emits fluorescence, and is a compound that can be observed at an excitation wavelength of 480 nm and a measurement wavelength of 575 nm.
- a tumor tissue specimen was prepared in the same manner as in Example 1 except that the tumor was removed from the mouse 20 minutes after doxorubicin administration and the thickness of the section was changed to 20 ⁇ m. Observation was performed with a confocal laser microscope (LEICA), and photography was performed.
- LICA confocal laser microscope
- Example 5 Relationship between normalization of tumor blood vessels by LPA and drug delivery with different molecular weights. Normalization of tumor blood vessels by administration of lysophospholipid was observed to improve the delivery of anticancer drugs. Regarding this drug delivery, whether the delivery is improved regardless of the difference in low molecular weight and high molecular weight is 70 kDa. Analysis was performed using two types of dextran, dextran and 2000 kDa dextran.
- dextran was administered into the tail vein under pentobarbital anesthesia 24 hours after LPA or PBS administration.
- Dextran 70 kDa and 2000 kDa was FITC-labeled dextran (SIGMA). Both dextrans were dissolved in PBS, diluted to 5 mg / mL, and 100 ⁇ L thereof was administered. Tumors were excised from the mice 30 minutes after dextran administration, and tumor tissue specimens were prepared in the same manner as in Example 4. Observation was performed with a confocal laser microscope (LEICA), and photography was performed.
- LICA confocal laser microscope
- Example 6 Examination of cancer treatment by combined use of LPA and anticancer agent
- LPA 3 mg / kg / 100 ⁇ L
- 5-FU 100 mg / kg / 100 ⁇ L
- PBS 100 ⁇ L
- both LPA and 5-FU were administered intraperitoneally.
- LPA or PBS was administered once a day for 7 days.
- 5-FU was administered once a week for a total of 2 times (7 days and 14 days after transplantation).
- Tumor size was measured over time after the start of administration. Tumor volume was calculated as major axis ⁇ minor axis ⁇ height ⁇ 0.5.
- mice were photographed with a digital camera under pentobarbital anesthesia. The tumor was removed and photographed with a digital camera.
- FIG. (A) is a figure which shows the time-dependent change of the tumor volume of each group
- (B) is a photograph of the external appearance of the representative mouse
- (C) is extracted from the representative mouse
- FIG. 6 the combined administration of 5-FU and LPA markedly suppressed tumor growth compared to the single administration of 5-FU. It was observed that with LPA alone, the tumor was reddish and blood flow increased. It was also shown that administration of LPA alone suppressed tumor growth compared to control.
- B16-BL6 cells a mouse melanoma-derived cell line
- B16-BL6 cells (1 ⁇ 10 6 cells / 100 ⁇ L / PBS) were injected subcutaneously into 8-week-old C57BL / 6NCrSlc mice. Seven days after LLC cell transplantation, mice with a tumor volume of 30-50 mm 3 were selected and subjected to the test.
- the subsequent experimental method is the same as that in 6-1 above.
- FIG. (A) is a figure which shows the time-dependent change of the tumor volume of each group
- (B) is a photograph of the typical mouse
- FIG. 7 the combined administration of 5-FU and LPA markedly suppressed tumor growth compared to the single administration of 5-FU. It was also shown that administration of LPA alone suppressed tumor growth compared to control.
- LPA since LPA alone suppressed tumor growth in any cell-derived tumor, LPA maintained tumor blood vessel permeability. As a result, cancer cells were attacked to induce cell death. It was thought that infiltration of immune cells such as natural killer cells and CD8 positive T cells into tumor tissues became possible. It was also considered that there was a possibility that a dominant increase in M1 macrophages showing antitumor activity was induced. That is, it was suggested that LPA administration enhances tumor immunity by improving blood flow in tumor blood vessels.
- Example 7 Induction of adhesion between vascular endothelial cells by LPA administration
- the tumor blood vessel luminal surface was changed to a smooth and gap-free state. Therefore, there is a possibility that adhesion between vascular endothelial cells is strengthened in the LPA administration group. It was. Therefore, the expression of VE-cadherin in tumor blood vessels after LPA administration, which is one of the molecules that induce cell-cell adhesion of vascular endothelial cells, was analyzed.
- a tumor immunostained tissue specimen was prepared in the same manner as in Example 1 except that the primary antibody and the secondary antibody were changed.
- the primary antibody Purified Goat anti-mouse VE-cadherin (BD Pharmigen), which is an anti-mouse VE-cadherin antibody, was diluted 200-fold with a blocking solution.
- Alexa Fluor 488 Goat Anti-Rat IgG (Life technologies) was diluted 400 times with a blocking solution.
- the prepared specimen was observed with a confocal laser microscope (LEICA) and photographed.
- a Z stack image was obtained with a confocal laser microscope, and the expression level (fluorescence intensity) of VE-cadherin was analyzed.
- FIG. (A) is a photograph of the specimen observed with a confocal laser microscope, in which VE-cadherin is stained with green fluorescence and depicted in white in the photograph.
- (B) is a chart showing a result of analyzing a VE-cadherin expression level (fluorescence intensity) by obtaining a Z stack image along the oblique white line portion of the photograph of (A). From the photograph below (A), it was observed that in the LPA group, VE-cadherin was gathered on the cell-cell adhesion surface. On the other hand, in the control group, it was observed that VE-cadherin was weakly expressed in the cells.
- the Z stack chart of (B) also showed that in the LPA administration group, the fluorescence intensity of the cell membrane portion was high, and a large amount of VE-cadherin was expressed in the cell membrane. From this result, it became clear that LPA induces the transfer of VE-cadherin, which is responsible for adhesion between vascular endothelial cells, to the cell membrane, thereby adhering vascular endothelial cells to each other.
- Example 8 Induction of adhesion between vascular endothelial cells by LPA derivative
- LPA increases adhesion between endothelial cells and induces improvement in the network of vascular endothelial cells and improvement in permeability from blood vessels.
- VPC31144S N- ⁇ (1S) -2-Hydroxy-1-[(Phosphonooxy) Methyl] Ethyl ⁇ (9Z) Octadec-9-enamide
- an LPA derivative also improves adhesion between endothelial cells. Whether or not the adhesion between the endothelial cells was analyzed using an electrical signal.
- a mouse pancreas-derived vascular endothelial cell line (MS-1) was used as the cell.
- a cell suspension is prepared with DMEM (Sigma) containing 5% FBS, seeded at 1 ⁇ 10 5 cells / 400 ⁇ L / well in an electrode well plate (8W10E +, 8 wells) dedicated to the above apparatus, and overnight. It was cultured in an incubator to make it confluent. After changing to a serum-free medium containing 0.1% BSA and incubating for 4 hours, the medium was replaced with a medium containing 10 ⁇ M LPA or VPC31144S (serum-free medium containing 0.1% BSA). The control was replaced with serum-free medium containing 0.1% BSA. After exchanging the medium, Rb, which is an index of cell gap resistance, was analyzed in real time to evaluate the barrier function.
- Example 9 Examination of suppression of cancer metastasis from primary lesion by LPA administration
- hypoxic conditions in tumor tissues may induce malignant transformation of cancer cells themselves and increase metastasis invasion of cancer cells. If the blood flow in the tumor is improved, the oxygen partial pressure does not decrease in the tumor, and the possibility of malignant cancer can be suppressed.
- the effect of LPA on cancer metastasis was examined using a melanoma cell line (B16-BL6 cells) having high metastatic potential.
- mice On day 42 after transplantation of B16-BL6 cells, the mice were euthanized and the lungs were removed. Using a stereomicroscope (LEICA), the number of metastatic colonies in the extracted lung was counted. Student's t-test was used for statistical analysis.
- LICA stereomicroscope
- Example 10 Expression analysis of LPA receptor in vascular endothelial cells of tumor tissue
- LLC cells were implanted subcutaneously into 8-week-old C57BL / 6NCrSlc mice (Aoi, SLC) to form tumors.
- the tumor was excised, and CD45 (blood marker) negative and CD31 (vascular endothelial cell marker) positive vascular endothelial cells were collected using FACS Aria (BD Bioscience).
- Total RNA was obtained from the collected vascular endothelial cells (CD45 ⁇ CD31 + ) using RNAeasy kit (Qiagen).
- mRNA expression level of LPA receptors (LPAR) 1 to 6 was determined by real-time PCR method. Analyzed by: As a control, the expression level of GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) mRNA, which is a glycolytic enzyme, was measured. Stratagene M ⁇ 300P (Stratagene) was used for real-time PCR.
- GAPDH Glyceraldehyde-3-phosphate dehydrogenase
- the primers used for real-time PCR are as follows. LPAR1 5'- CCGCTTCCATTTCCCTATTT -3 '(SEQ ID NO: 1) 5'- AAAACCGTGATGTGCCTCTC -3 '(SEQ ID NO: 2) LPAR2 5'- CCATCAAAGGCTGGTTCCT -3 '(SEQ ID NO: 3) 5′-TCCAAGTCACAGAGGCAGTG -3 ′ (SEQ ID NO: 4) LPAR3 5'-TTCCACTTTCCCTTCTACTACCTG-3 '(SEQ ID NO: 5) 5′-TCCACAGCAATAACCAGCAA-3 ′ (SEQ ID NO: 6) LPAR4 5'-GCCCTCTCTGATTTGCTTTT-3 '(SEQ ID NO: 7) 5'-TCCTCCTGGTCCTGATGGTA -3 '(SEQ ID NO: 8) LPAR5 5'-AGCGATGAACTGTGGAAGG-3 '(SEQ ID NO: 9) 5′
- Example 11 Expression analysis of LPA receptor and functional analysis of LPAR4 in vascular endothelial cell line
- MS-1 cells mouse pancreatic vascular endothelial cell line
- LPAR LPA receptor
- the expression level was analyzed by real-time PCR method. The results are shown in FIG.
- the expression level of each LPA receptor is shown as a relative value to the expression level of GAPDH.
- LPAR4 and LPAR6 were expressed in MS-1 cells, and the expression levels of LPAR1, LPAR2, LPAR3 and LPAR5 were below the detection limit.
- LPAR4 LPAR4-specific siRNA (siRNA ID: s95367, life Technologies) was introduced into MS-1 cells using lipofectamine 2000 (Invitrogen), and LPAR4 was knocked down. MS-1 cells introduced with control siRNA were used as control cells. Control cells and LPAR4 knockdown cells were seeded at 2.5 ⁇ 10 5 cells / 2 mL in glass bottom dishes (Iwaki) each coated with collagen type I, and cultured for 3 days until the cells became confluent. After washing the cells with PBS for 1 minute ⁇ 3 times, a blocking solution (5% normal goat serum / 1% BSA / 2% skim milk / PBS) was dropped on the dish, and blocking was performed at room temperature for 30 minutes.
- a blocking solution 5% normal goat serum / 1% BSA / 2% skim milk / PBS
- Example 12 Effect of LPA on diseases in which new blood vessels with increased vascular permeability are formed
- Lower limb ischemia model is used as a pathological model of Buerger's disease and chronic obstructive arteriosclerosis. That is, when the blood vessels of the mouse lower limbs are excised, ischemia occurs in the vascular control region, and inflammation is brought about by the surgical technique, resulting in angiogenesis, resulting in increased permeability of the new blood vessels. Edema occurs in the muscle area. It was examined whether LPA has an effect of suppressing the increase in permeability of new blood vessels due to such inflammation and ischemia.
- LPA 3 mg / kg / 100 ⁇ L
- PBS 100 ⁇ L
- Administration was performed once a day for 3 consecutive days for a total of 3 times. Thigh circumference was measured 24 hours after the last dose.
- Age-related macular degeneration model Exudative age-related macular degeneration is an ocular disease caused by choroidal neovascularization and is the leading cause of blindness in developed countries. Unlike healthy blood vessels, choroidal neovascularization has increased vascular permeability, and blood components leak, causing retinal edema and subretinal fluid, causing visual impairment. Currently, drugs that inhibit VEGF involved in the development and proliferation of choroidal neovascularization have been clinically applied, but there is still no cure that completely restores visual function, and the development of a more effective treatment is desired. . Therefore, it was examined whether LPA can suppress this increase in permeability of choroidal neovascularization.
- LPA 3 mg / kg / 100 ⁇ L
- PBS 100 ⁇ L
- FITC-labeled 70 kDa dextran SIGMA
- the collected eyeball was immersed in 4% paraformaldehyde (PFA) / PBS for 2 hours, and then the cornea, lens, and retina were removed from the eyeball to produce a choroidal flat mount. Then, it wash
- PFA paraformaldehyde
- Purified Hamster Anti-PECAM-1 (MILLIPORE: MAB1398Z), which is an anti-mouse CD31 antibody, was diluted 200-fold with a blocking solution, dropped onto a section, and reacted at 4 ° C. overnight. . Washing with PBST for 30 minutes was performed 6 times, followed by washing with PBS for 30 minutes. Alexa Fluor 488 Goat Anti-Hamster IgG (Jackson ImmunoResearch Labolatories) was used as the secondary antibody, diluted 400-fold with a blocking solution, dropped onto a section, and allowed to react for 6 hours in the dark. Washing with PBST for 30 minutes was performed 6 times, a few drops of Vectashield were dropped and sealed with a cover glass. The images were taken with a confocal laser microscope.
- the results are shown in FIG.
- the upper row (CD31) is a photograph of a tissue specimen obtained by immunostaining vascular endothelial cells with an anti-CD31 antibody. The vascular endothelial cells are stained with green fluorescence and depicted in white in the photograph.
- the lower row (Dextran) is a photograph observing the fluorescence of dextran leaked from a new blood vessel. As shown in the upper row, no significant difference was found between the LPA group and the control group regarding the size of choroidal neovascularization.
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Abstract
Description
[1]血管形成異常を伴う疾患の治療薬の疾患部位への送達を促進するための薬剤送達促進剤であって、リゾリン脂質受容体を活性化する物質を有効成分として含有し、血管形成異常を伴う疾患の治療薬と組み合わせて使用することを特徴とする薬剤送達促進剤。
[2]リゾリン脂質受容体を活性化する物質がリゾリン脂質もしくはその前駆体、またはそれらの誘導体である前記[1]に記載の薬剤送達促進剤。
[3]血管形成異常を伴う疾患が固形がん、加齢黄斑変性症、関節リウマチ、乾癬、強皮症、全身性エリテマトーデス、血管炎症候群、血管ベーチェット、糖尿病性網膜症、糖尿病性腎症、動脈硬化症、慢性閉塞性動脈硬化症、バージャー病、肺線維症、脳梗塞、重症感染症または心不全である前記[1]または[2]に記載の薬剤送達促進剤。
[4]血管形成異常を伴う疾患が固形がんである前記[3]に記載の薬剤送達促進剤。
[5]リゾリン脂質がリゾホスファチジン酸、リゾホスファチジルセリン、リゾホスファチジルコリン、リゾホスファチジルエタノールアミン、リゾホスファチジルイノシトール、リゾホスファチジルグリセロール、スフィンゴシン-1-リン酸、スフィンゴシルホスホリルコリンおよび血小板活性化因子(PAF)から選択される1種または2種以上である前記[1]~[4]のいずれかに記載の薬剤送達促進剤。
[6]リゾリン脂質がリゾホスファチジン酸である前記[5]に記載の薬剤送達促進剤。
[7]リゾリン脂質受容体を活性化する物質を有効成分として含有する血管形成異常を伴う疾患の治療用医薬組成物。
[8]リゾリン脂質受容体を活性化する物質がリゾリン脂質もしくはその前駆体、またはそれらの誘導体である前記[7]に記載の医薬組成物。
[9]腫瘍増殖抑制作用、がん転移抑制作用、腫瘍免疫亢進作用および血管透過性亢進抑制作用から選択される1種以上の作用を有する前記[8]に記載の医薬組成物。
[10]血管形成異常を伴う疾患治療用の他の薬剤と組み合わせて使用することを特徴とする前記[8]または[9]に記載の医薬組成物。
[11]血管形成異常を伴う疾患治療用の他の薬剤が、がん治療薬である前記[10]に記載の医薬組成物。
[12]組み合わせて使用する他の薬剤の血管形成異常を伴う疾患部位への送達を促進するための前記[10]または[11]に記載の医薬組成物。
[13]血管形成異常を伴う疾患が固形がん、加齢黄斑変性症、関節リウマチ、乾癬、強皮症、全身性エリテマトーデス、血管炎症候群、血管ベーチェット、糖尿病性網膜症、糖尿病性腎症、動脈硬化症、慢性閉塞性動脈硬化症、バージャー病、肺線維症、脳梗塞、重症感染症または心不全である前記[7]~[12]のいずれかに記載の医薬組成物。
[14]血管形成異常を伴う疾患が固形がんである前記[13]に記載の医薬組成物。
[15]リゾリン脂質がリゾホスファチジン酸、リゾホスファチジルセリン、リゾホスファチジルコリン、リゾホスファチジルエタノールアミン、リゾホスファチジルイノシトール、リゾホスファチジルグリセロール、スフィンゴシン-1-リン酸、スフィンゴシルホスホリルコリンおよび血小板活性化因子(PAF)から選択される1種または2種以上である前記[7]~[14]のいずれかに記載の医薬組成物。
[16]リゾリン脂質がリゾホスファチジン酸である前記[15]に記載の医薬組成物。
[17]血管形成異常を伴う疾患の治療薬のスクリーニング方法であって、疾患部位の血管内皮細胞に特異的に発現しているリゾリン脂質受容体を活性化する物質を選択することを特徴とする方法。
本発明は、血管形成異常を伴う疾患の治療薬の疾患部位への送達を促進するための薬剤送達促進剤を提供する。本発明の薬剤送達促進剤は、リゾリン脂質受容体を活性化する物質を有効成分として含有し、血管形成異常を伴う疾患の治療薬と組み合わせて使用することを特徴とする。血管形成異常を伴う疾患部位では、血流が乏しいことに加え、血管透過性が過剰な亢進状態にあることが知られている。例えば固形がんでは、腫瘍間質圧を増加させ、腫瘍間質内と血管内の浸透圧の差がなくなり、血管内腔から腫瘍組織内への物質の移動が困難となっていることが知られている。本発明の薬剤送達促進剤は、血管形成異常を伴う疾患部位の異常血管を正常化し、血管透過性を正常化することができるので、組み合わせて使用する薬剤の疾患部位への送達効率を顕著に高めることができる。
本発明は、リゾリン脂質受容体を活性化する物質を有効成分として含有する血管形成異常を伴う疾患の治療用医薬組成物を提供する。上記本発明の薬剤送達促進剤は、医薬品の形態で実施することが好ましい。本発明の医薬組成物は、単独で使用してもよいし、血管形成異常を伴う疾患治療用の他の薬剤と組み合わせて使用してもよい。リゾリン脂質受容体を活性化する物質はリゾリン脂質に限定されるものではなく、リゾリン脂質の誘導体、リゾリン脂質の前駆体またはそれらの誘導体等も有効成分として好適に用いることができる。さらに、これら以外のリゾリン脂質受容体アゴニスト(例えば、低分子化合物、核酸、ペプチド、蛋白質、抗体等)も有効成分として好適に用いることができる。公知のリゾリン脂質受容体アゴニストとしては、例えばWongらの論文(Assay Drug Dev Technol. 2010 Aug;8(4):459-70. doi: 10.1089/adt.2009.0261.)に記載のLPA4受容体アゴニストなどが挙げられる。好ましくは、リゾリン脂質もしくはその前駆体、またはそれらの誘導体である。
投与量は、投与対象、症状、投与ルートなどにより差異はあるが、経口投与の場合、一般的に例えば、体重約60kgのヒトにおいては、1日当たり約0.01~1000mg、好ましくは約0.1~100mg、より好ましくは約0.5~500mgである。
非経口投与の合は、その1回投与量は患者の状態、症状、投与方法などによっても異なるが、例えば注射剤では、通常例えば体重1kg当たり約0.01~100mg、好ましくは約0.01~50mg、より好ましくは約0.01~20mgを静脈に投与する。1日当たりの総投与量は、単一投与量であっても分割投与量であってもよい。
化学療法剤としては、特に限定されないが、例えばナイトロジェンマスタード、塩酸ナイトロジェンマスタード-N-オキシド、クロラムブチル、シクロフォスファミド、イホスファミド、チオテパ、カルボコン、トシル酸インプロスルファン、ブスルファン、塩酸ニムスチン、ミトブロニトール、メルファラン、ダカルバジン、ラニムスチン、リン酸エストラムスチンナトリウム、トリエチレンメラミン、カルムスチン、ロムスチン、ストレプトゾシン、ピポブロマン、エトグルシド、カルボプラチン、シスプラチン、ミボプラチン、ネダプラチン、オキサリプラチン、アルトレタミン、アンバムスチン、塩酸ジブロスピジウム、フォテムスチン、プレドニムスチン、プミテパ、リボムスチン、テモゾロミド、トレオスルファン、トロフォスファミド、ジノスタチンスチマラマー、アドゼレシン、システムスチン、ビゼレシン等のアルキル化剤;例えば、メルカプトプリン、6-メルカプトプリンリボシド、チオイノシン、メトトレキサート、ペメトレキセド、エノシタビン、シタラビン、シタラビンオクフォスファート、塩酸アンシタビン、5-FU系薬剤(例、フルオロウラシル、テガフール、UFT、ドキシフルリジン、カルモフール、ガロシタビン、エミテフール、カペシタビン等)、アミノプテリン、ネルザラビン、ロイコボリンカルシウム、タブロイド、ブトシン、フォリネイトカルシウム、レボフォリネイトカルシウム、クラドリビン、エミテフール、フルダラビン、ゲムシタビン、ヒドロキシカルバミド、ペントスタチン、ピリトレキシム、イドキシウリジン、ミトグアゾン、チアゾフリン、アンバムスチン、ベンダムスチン等の代謝拮抗剤;例えば、アクチノマイシンD、アクチノマイシンC、マイトマイシンC、クロモマイシンA3、塩酸ブレオマイシン、硫酸ブレオマイシン、硫酸ペプロマイシン、塩酸ダウノルビシン、塩酸ドキソルビシン、塩酸アクラルビシン、塩酸ピラルビシン、塩酸エピルビシン、ネオカルチノスタチン、ミスラマイシン、ザルコマイシン、カルチノフィリン、ミトタン、塩酸ゾルビシン、塩酸ミトキサントロン、塩酸イダルビシン等の抗がん性抗生物質;例えば、エトポシド、リン酸エトポシド、硫酸ビンブラスチン、硫酸ビンクリスチン、硫酸ビンデシン、テニポシド、パクリタキセル、ドセタクセル、ビノレルビン、イリノテカン、塩酸イリノテカン等の植物由来抗がん剤などが挙げられる。
本発明は、血管形成異常を伴う疾患の治療薬のスクリーニング方法を提供する。本発明のスクリーニング方法は、疾患部位の血管内皮細胞に特異的に発現しているリゾリン脂質受容体を活性化する物質(リゾリン脂質受容体アゴニスト)を選択することを特徴としている。
血管形成異常を伴う疾患の治療薬の疾患部位への送達を促進する方法であって、哺乳動物に対してリゾリン脂質もしくはその前駆体、またはそれらの誘導体の有効量を投与し、さらに血管形成異常を伴う疾患の治療薬の有効量を投与することを特徴とする薬剤送達促進方法。
哺乳動物に対してリゾリン脂質もしくはその前駆体、またはそれらの誘導体の有効量を投与し、さらに血管形成異常を伴う疾患の治療薬の有効量を投与することを特徴とする血管形成異常を伴う疾患の治療方法。
哺乳動物に対してリゾリン脂質もしくはその前駆体、またはそれらの誘導体の有効量を投与することを特徴とする固形がんの治療方法。
哺乳動物に対してリゾリン脂質もしくはその前駆体、またはそれらの誘導体の有効量を投与することを特徴とするがんの転移抑制方法。
血管形成異常を伴う疾患の治療薬の疾患部位への送達促進に使用するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体。
血管形成異常を伴う疾患の治療に使用するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体。
固形がんの治療に使用するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体。
がんの転移抑制に使用するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体。
血管形成異常を伴う疾患の治療薬の疾患部位への送達を促進するための薬剤送達促進剤を製造するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体の使用。
血管形成異常を伴う疾患の治療薬を製造するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体の使用。
固形がんの治療薬を製造するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体の使用。
がんの転移抑制薬を製造するための、リゾリン脂質もしくはその前駆体、またはそれらの誘導体の使用。
マウスがん細胞株をマウス皮下に移植して腫瘍を形成させた後にLPAを投与し、LPAが血管に対してどのような構造的変化を誘導するかを観察した。
(1)実験方法
マウスがん細胞株としてLewis肺癌細胞株(以下、「LLC細胞」という。)を用いた。8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下に、LLC細胞(1×106個/100μL・PBS/匹)を注射した。
LPAには、18:1LPA(Avanti POLAR LIPIDS社)を使用した。50%エタノールを用いて10mMのLPAストック溶液を調製し、-30℃で保存した。使用時に解凍し、超音波洗浄機(エスエヌディ社)で1分間細分化した後、PBSで3mg/kg/100μLとなるように投与用LPA溶液を用時調製した。
結果を図1に示した。(A)は共焦点レーザー顕微鏡で標本を観察した写真であり、血管内皮細胞が緑色蛍光に染色され、写真では白く描出されている。(B)はAngioTool血管構造解析ソフトで算出した各群の平均血管長を示す図である。図1(A)に示したように、LPA投与前の腫瘍血管(control)は網目構造が疎で血管が不連続であったが、6時間後では血管の連結状態が観察されるようになり、12時間、24時間後では正常組織と同様に血管の網状構造が観察された。図1(B)に示したように、LPAの投与により一本一本の血管の長さが長くなっていることが数値として示された。この結果は、LPA投与後短時間で腫瘍血管のネットワーク構築が誘導されることを示している。したがって、LPAによる腫瘍血管のネットワーク構築は血管内皮細胞の増殖に基づくものとは考え難く、血管内皮細胞の伸長/接着/管腔形成に基づくものと考えられた。なお、結果を示していないが、がん細胞株としてLLC細胞以外のcolon26大腸がん細胞やB16メラノーマ細胞を用いた実験においても同様の結果が得られた。
LPA以外のリゾリン脂質であるS1Pを用いて、LPAと同様に腫瘍血管のネットワーク構築が誘導されるかどうかを観察した。
(1)実験方法
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下にLLC細胞を移植した。S1P(Avanti POLAR LIPIDS社)はPBSで10mMとなるように調製し、これをストック溶液として-30℃で保存した。使用時に解凍し、超音波洗浄機(エスエヌディ社)で1分間細分化した後、PBSで0.3mg/kg/100μLとなるように投与用S1P溶液を用時調製した。
結果を図2に示した。LPAを投与した場合と同様に、S1Pを投与した場合も腫瘍血管のネットワーク構築が誘導されることが明らかになった。この結果から、腫瘍血管のネットワーク構築を誘導して正常化させることができるのはLPAに限定されず、他のリゾリン脂質を用いても同じ効果を奏することが判明した。
(1)実験方法
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下にLLC細胞を移植した。また、実施例1と同じ方法で投与用のLPAを調製した。LLC細胞移植後9日目のマウス(腫瘍体積が60~80mm3になった個体)を実験に供し、コントロール群とLPA群の2群に分けた(n=3)。群分け後、LPA(3mg/kg/100μL)またはPBS(100μL)を腹腔内投与した。LPAまたはPBS投与の24時間後に、ペントバルビタール(共立製薬株式会社)麻酔下で、マウスを潅流固定した。固定液には、2%ホルムアルデヒドおよび2.5%グルタールアルデヒドを含む0.1Mリン酸緩衝液(pH7.4)を用いた。還流固定後、腫瘍を摘出し、潅流に用いたものと同じ固定液に浸漬して4℃で一晩振盪した。さらに1%四酸化オスミウムおよび0.5%フェロシアンカリウムを含む0.1Mリン酸緩衝液(pH7.4)に浸漬して固定した。濃度上昇系列エタノールで脱水し、t-ブチルアルコールに置換して凍結乾燥を行った。凍結乾燥後、四酸化オスミウムを蒸着し、日立ハイテク製の走査型電子顕微鏡S-4800で、血管内腔面を観察した。
結果を図3に示した。図3から明らかなように、コントロール群の血管内腔面には糸状仮足を伸ばしたような歪な構造が観察されたが、LPA投与後の血管内腔は非常に平滑な構造が観察された。この結果から、LPAを投与することにより腫瘍内の血液の循環が改善することが予想された。
従来から知られているように、腫瘍内は血流が乏しいことに加え、血管透過性が過剰な亢進状態にある。そのため、最終的に腫瘍間質圧を増加させ、腫瘍間質内と血管内の浸透圧の差がなくなり、血管内腔から腫瘍組織内への物質の移動が困難となっている。LPA投与後に腫瘍内の血管網が密に構築され、血管内腔も平滑になることが明らかになったため、LPA投与後の腫瘍血管では薬剤の透過性が改善していることが予想された。そこで、LPA投与後の腫瘍における薬剤の透過性を確認するために、以下の実験を行った。
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下にLLC細胞を移植した。また、実施例1と同じ方法で投与用のLPAを調製した。LLC細胞移植後11日目に、腫瘍体積が100~120mm3になったマウスを選択し、コントロール群とLPA群の2群に分けた(n=3)。群分け後、LPA(3mg/kg/100μL)またはPBS(100μL)を腹腔内投与した。LPAまたはPBS投与の24時間後に、ペントバルビタール麻酔下で、ドキソルビシン(ドキソルビシン塩酸塩、日本化薬株式会社)を1.5mg/kgの用量でマウスに尾静脈内に投与した。ドキソルビシンは生理食塩水(大塚製薬株式会社)に溶解し、1.5mg/kgとなるように希釈した後、超音波洗浄機で1分間細分化を行ってから投与した。なお、ドキソルビシンは蛍光を発する抗癌剤であり、励起波長480nm、測定波長575nmで観察することが可能な化合物である。ドキソルビシン投与の20分後にマウスから腫瘍を摘出し、切片の厚さを20μmに変更した以外は実施例1と同じ方法で腫瘍の組織標本を作製した。共焦点レーザー顕微鏡(LEICA社)にて観察し、写真撮影を行った。
結果を図4に示した。図4中、矢印はドキソルビシンの赤色蛍光シグナルを示す。血管内皮細胞は、抗CD31抗体により緑色蛍光を発している。図4から明らかなように、コントロールではドキソルビシンの腫瘍内移行はほとんど観察されなかったが、LPAを投与後の腫瘍においては、ドキソルビシンが血管内から腫瘍深部に送達されていることが観察された。
リゾリン脂質投与による腫瘍血管の正常化により抗がん剤の送達性の改善が観察されたが、この薬剤送達に関して、低分子量、高分子量の違いに関わらず送達性が改善するのかどうかを、70kDaデキストランと2000kDaデキストランの2種類のデキストランを用いて解析した。
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下にLLC細胞を移植した。また、実施例1と同じ方法で投与用のLPAを調製した。LLC細胞移植後11日目に、腫瘍体積が100~120mm3になったマウスを選択し、70kDaデキストラン投与のコントロール群とLPA群、および2000kDaデキストラン投与のコントロール群とLPA群の合計4群に分けた(n=3)。群分け後、LPA(3mg/kg/100μL)またはPBS(100μL)を腹腔内投与した。LPAまたはPBS投与の24時間後に、ペントバルビタール麻酔下で、各デキストランを尾静脈内に投与した。デキストラン(70kDaおよび2000kDa)は、FITC標識されたデキストラン(SIGMA社)を使用した。どちらのデキストランもPBSに溶解し、5mg/mLとなるように希釈して、その100μLを投与した。デキストラン投与の30分後にマウスから腫瘍を摘出し、実施例4と同じ方法で腫瘍の組織標本を作製した。共焦点レーザー顕微鏡(LEICA社)にて観察し、写真撮影を行った。
結果を図5に示した。70kDaデキストランおよび2000kDaデキストランのいずれも、コントロール群では腫瘍内移行がほとんど観察されなかったが、LPA投与群では腫瘍深部に送達されていることが観察された。この結果から、LPA投与によって腫瘍血管の透過性が正常化されることにより、低分子化合物に限らず、高分子化合物(例えば、核酸、抗体等)も、腫瘍深部に送達可能になると考えられる。
LPA投与により、抗がん剤を腫瘍深部まで効率よく送達するできることが示されたため、LPAと抗がん剤の併用によるがん治療を試みた。
(1)実験方法
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウスの皮下にLLC細胞を移植した。また、実施例1と同じ方法で投与用のLPAを調製した。抗癌剤として5-FU(協和発酵キリン社)を使用した。5-FUの調製には生理食塩水(大塚製薬株式会社)を用いた。LLC細胞移植後7日目に、腫瘍体積が30~50mm3になったマウスを選択し、試験に供した。コントロール群、5-FU群、LPA群および5-FU/LPA群の4群を設けた(n=3)。群分け後、LPA(3mg/kg/100μL)または5-FU(100mg/kg/100μL)またはPBS(100μL)を腹腔内投与した。5-FU/LPA群にはLPAと5-FUの両方を腹腔内投与した。LPAまたはPBSは1日1回7日間連日投与した。5-FUは週1回、合計2回(移植後7日目と14日目)に投与した。投与開始後経時的に腫瘍サイズを測定した。腫瘍体積は、長径×短径×高さ×0.5で計算した。投与開始から2週間後のマウスをペントバルビタール麻酔下で、デジタルカメラで撮影した。また、腫瘍を摘出し、デジタルカメラで撮影した。
結果を図6に示した。(A)は各群の腫瘍体積の経時変化を示す図であり、(B)は各群の代表的なマウスの外観の写真であり、(C)は各群の代表的なマウスから摘出した腫瘍の写真である。図6から明らかなように、5-FUとLPAの併用投与は、5-FUの単独投与より腫瘍の増大を顕著に抑制した。LPAの単独投与では、腫瘍が赤みを帯びており、血流が増加していることが観察された。また、LPAの単独投与でも、コントロールと比較して腫瘍の増大を抑制することが示された。
(1)実験方法
マウスメラノーマ由来細胞株のB16-BL6細胞を用いた。8週齢のC57BL/6NCrSlcマウスの皮下に、B16-BL6細胞(1×106個/100μL・PBS/匹)を注射した。LLC細胞移植後7日目に、腫瘍体積が30~50mm3になったマウスを選択し、試験に供した。以後の実験方法は、上記6-1と同じである。
結果を図7に示した。(A)は各群の腫瘍体積の経時変化を示す図であり、(B)は各群の代表的なマウスの写真である。図7から明らかなように、5-FUとLPAの併用投与は、5-FUの単独投与より腫瘍の増大を顕著に抑制した。また、LPAの単独投与でも、コントロールと比較して腫瘍の増大を抑制することが示された。
(1)実験方法
マウス大腸がん細胞株のcolon26細胞を用いた。8週齢のBALB/cマウス(♀、SLC社)の皮下に、colon26細胞(1×106個/100μL・PBS/匹)を注射した。LLC細胞移植後7日目に、腫瘍体積が30~50mm3になったマウスを選択し、試験に供した。以後の実験方法は、上記6-1と同じである。
各群の腫瘍体積の経時変化の結果を図8に示した。図8から明らかなように、5-FUとLPAの併用投与は、5-FUの単独投与より腫瘍の増大を顕著に抑制した。また、移植後17日目までは、LPAの単独投与でもコントロールと比較して腫瘍の増大を抑制することが示された。
(1)実験方法
5-FUに代えてオキサリプラチンを用いた以外は、上記6-3と同じ方法で実施した。オキサリプラチン(コスモバイオ株式会社)の調製には生理食塩水(大塚製薬株式会社)を用い、1.5mg/kg/100μLを腹腔内投与した。
各群の腫瘍体積の経時変化の結果を図9に示した。図9から明らかなように、オキサリプラチンとLPAの併用投与は、オキサリプラチンの単独投与より腫瘍の増大を顕著に抑制した。また、移植後17日目までは、LPAの単独投与でもコントロールと比較して腫瘍の増大を抑制することが示された。この結果から、LPAと抗がん剤の併用効果は、抗がん剤として5-FUを用いた場合に限らないことが示された。
以上より、LPAの腫瘍血管に対する効果は、あらゆるがん細胞によるがん組織に有効であることが示唆された。
実施例3において、LPA投与群では腫瘍血管内腔面が平滑で隙間のない状態に変化していたことから、LPA投与群では血管内皮細胞間の接着が強固になっている可能性が考えられた。そこで、血管内皮細胞の細胞間接着を誘導する分子の1つである、LPA投与後の腫瘍血管におけるVE-カドヘリンの発現を解析した。
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下にLLC細胞を移植した。また、実施例1と同じ方法で投与用のLPAを調製した。LLC細胞移植後9日目のマウス(腫瘍体積が60~80mm3になった個体)を実験に供し、コントロール群とLPA群の2群に分けた(n=3)。群分け後、LPA群のマウスにLPAを3mg/kg/100μLの用量で腹腔内投与した。コントロール群は群分け後に腫瘍を摘出し、LPA群はLPA投与の24時間後に腫瘍を摘出した。腫瘍の免疫染色組織標本は、1次抗体および2次抗体を変更した以外実施例1と同じ方法で作製した。1次抗体には、抗マウスVE-カドヘリン抗体であるPurified Goat anti-mouse VE-cadherin(BD pharmigen社)をブロッキング溶液で200倍希釈して用いた。2次抗体には、Alexa Fluor 488 Goat Anti-Rat IgG(Life technologies社)をブロッキング溶液で400倍希釈して用いた。作製した標本を共焦点レーザー顕微鏡(LEICA社)にて観察し、写真撮影を行った。また、共焦点レーザー顕微鏡にてZスタック画像を取得し、VE-カドヘリン発現量(蛍光強度)を解析した。
結果を図10に示した。(A)は共焦点レーザー顕微鏡で標本を観察した写真であり、VE-カドヘリンが緑色蛍光に染色され、写真では白く描出されている。(B)は(A)の写真の斜め白線部分に沿ってZスタック画像を取得し、VE-カドヘリン発現量(蛍光強度)を解析した結果を示すチャートである。(A)の下の写真から、LPA群ではVE-カドヘリンが細胞と細胞の接着面に集まっていることが観察された。一方、コントロール群では、VE-カドヘリンは細胞内に弱く発現していることが観察された。(B)のZスタックチャートを見ても、LPA投与群では、細胞膜部分の蛍光強度が高く、細胞膜にVE-カドヘリンが多く発現していることが示された。この結果から、LPAが血管内皮細胞同士の接着を担うVE-カドヘリンの細胞膜への移行を誘導して、血管内皮細胞同士を接着させることが明らかとなった。
LPAにより内皮細胞同士の接着性が高まり、血管内皮細胞のネットワークの改善や、血管からの透過性の改善が誘導されることが、上記実施例7で明らかになった。そこで、LPA誘導体であるVPC31144S(N-{(1S)-2-Hydroxy-1-[(Phosphonooxy) Methyl]Ethyl}(9Z) Octadec-9-enamide)によっても、内皮細胞同士の接着性が向上するかどうかを、内皮細胞同士の接着を電気信号で解析する方法を用いて解析した。
LPAおよびVPC31144Sのin vitroにおける血管内皮細胞同士の細胞接着をバリア機能として解析するために、リアルタイム細胞解析装置ECIS-Zθ(Applied Biophysic 社製)を使用した。
LPAおよびVPC31144SはAvanti POLAR LIPIDS社製を使用した。VPC31144SはLPAR4にアゴニスト作用を示すことが知られているLPA誘導体である。LPAおよびVPC31144Sは、それぞれ50%エタノールを用いて10mMのLPAストック溶液を調製し、-30℃で保存した。使用時に解凍し、超音波洗浄機(エスエヌディ社)で1分間細分化した後、無血清培地にBSAを0.1%加えた培地で10μMに調製した。
結果を図11に示した。図11のX軸は時間経過、Y軸は電気抵抗(Rb)を示す。Rbが高いほど、細胞間接着が強いことを示す。LPA添加により、コントロールに比べ電気信号が高まり、長期間にわたって内皮細胞同士のバリア機能が強くなることが示された。同様に、VPC31144を添加した場合も内皮細胞同士のバリア機能が強くなることが示された。さらに、VPC31144を添加した場合は、LPA添加と比較して、添加直後より強いバリア機能が発揮されることが明らかになった。この結果から、LPAに限らず、LPAR4にアゴニスト作用を有するLPA誘導体も、血管形成異常を伴う疾患に対する透過性の改善や血管網の正常化に有用であることが判明した。
これまでの報告から、腫瘍組織の低酸素状態はがん細胞そのものの悪性化を誘導し、がん細胞の転移浸潤を旺盛にする可能性が示唆されている。腫瘍内の血流が改善されれば、酸素分圧も腫瘍内で低下せず、がんの悪性化が抑制できる可能性が考えられる。さらに、上記したように内皮細胞同士が接着した状態で維持されることで、血管内へのがん細胞の移動侵入が困難になり、転移が抑制される可能性も考えられる。そこで、高転移能を有するメラノーマ細胞株(B16-BL6細胞)を用いて、LPAのがん転移に対する効果を検討した。
8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下に、B16-BL6細胞(1×106個/100μL・PBS/匹)を注射した。B16-BL6細胞移植後7日目に、腫瘍体積が30~50mm3になったマウスを選択し、コントロール群とLPA群の2群に分けた(n=3)。群分け後、LPA(3mg/kg/100μL)またはPBS(100μL)を腹腔内投与した。LPAまたはPBSは移植後21日目まで1日1回連日投与した。B16-BL6細胞を移植後42日目に、マウスを安楽死させ肺を摘出した。実体顕微鏡(LEICA社)を用いて、摘出した肺の転移コロニー数を計測した。統計解析には、Student’s t-testを用いた。
結果を図12に示した。図12から明らかなように、LPA群はコントロール群と比較して、有意に肺への転移を抑制した(p<0.01)。この結果から、LPAによる腫瘍血流改善および血管内皮細胞の接着誘導は、がん細胞の悪性化および転移を抑制することが確認された。
(1)実験方法
実施例1と同じ方法で8週齢のC57BL/6NCrSlcマウス(♀、SLC社)の皮下にLLC細胞を移植し、腫瘍を形成させた。腫瘍を摘出し、CD45(血液マーカー)陰性、CD31(血管内皮細胞マーカー)陽性の血管内皮細胞をFACS Aria(BD Bioscience社)を用いて回収した。回収した血管内皮細胞(CD45-CD31+)から、RNAeasy kit(Qiagen社)を用いてトータルRNAを得た。次に、ExScript RT reagent Kit(タカラバイオ社)を用いて、トータルRNAからcDNAを合成し、得られたcDNAを用いて、LPA受容体(LPAR)1~6のmRNAの発現量をリアルタイムPCR法によって解析した。対照として解糖系酵素であるGAPDH(Glyceraldehyde-3-phosphate dehydrogenase)のmRNAの発現量を測定した。リアルタイムPCRには、Stratagene M×300P(Stratagene社製)を用いた。
LPAR1
5'- CCGCTTCCATTTCCCTATTT -3'(配列番号1)
5'- AAAACCGTGATGTGCCTCTC -3'(配列番号2)
LPAR2
5'- CCATCAAAGGCTGGTTCCT -3'(配列番号3)
5'- TCCAAGTCACAGAGGCAGTG -3'(配列番号4)
LPAR3
5'- TTCCACTTTCCCTTCTACTACCTG -3'(配列番号5)
5'- TCCACAGCAATAACCAGCAA -3'(配列番号6)
LPAR4
5'- GCCCTCTCTGATTTGCTTTT -3'(配列番号7)
5'- TCCTCCTGGTCCTGATGGTA -3'(配列番号8)
LPAR5
5'- AGCGATGAACTGTGGAAGG -3'(配列番号9)
5'- GCAGGAAGATGATGAGATTGG -3'(配列番号10)
LPAR6
5'- TGTGCCCTACAACATCAACC-3'(配列番号11)
5'- TCACTTCTTCTAACCGACCAG -3'(配列番号12)
GAPDH
5'- AACTTTGGCATTGTGGAAGG -3'(配列番号13)
5'- GGATGCAGGGATGATGTTCT -3'(配列番号14)
結果を図13に示した。各LPA受容体の発現量は、GAPDHの発現量に対する相対値で示した。図13から明らかなように、腫瘍組織の血管内皮細胞では、LPAR1、LPAR4およびLPAR6が発現しており、LPAR2、LPAR3およびLPAR5の発現量は検出限界以下であった。
(1)LPA受容体の発現解析
実施例9と同じ方法で、MS-1細胞(マウス膵臓由来血管内皮細胞株)から、トータルRNAを回収し、LPA受容体(LPAR)1~6のmRNAの発現量をリアルタイムPCR法によって解析した。
結果を図14に示した。各LPA受容体の発現量は、GAPDHの発現量に対する相対値で示した。図14から明らかなように、MS-1細胞では、LPAR4およびLPAR6が発現しており、LPAR1、LPAR2、LPAR3およびLPAR5の発現量は検出限界以下であった。
LPAR4特異的siRNA(siRNA ID: s95367、life Technologies社)を、lipofectamine2000(invitrogen社)を用いてMS-1細胞に導入し、LPAR4をノックダウンした。コントロールsiRNAを導入したMS-1細胞をコントロール細胞とした。コントロール細胞とLPAR4ノックダウン細胞を、それぞれコラーゲンタイプIをコーティングしたガラスボトムディッシュ(Iwaki社)に2.5×105個/2mLで播種し、細胞がコンフルエントになるまで3日間培養した。細胞をPBSで1分間×3回洗浄した後、ブロッキング溶液(5% normal goat serum/1%BSA/2%skim milk/PBS)をディッシュ上に滴下し、室温で30分間ブロッキングを行った。1次抗体にはPurified Goat anti-mouse VE-cadherin(BD pharmigen社)を用い、4℃で一晩反応させた。Triton X-100含有PBS(PBST)で10分の洗浄を3回行った後、二次抗体Alexa Fluor 488 goat anti-rat IgG(Molecular Probes社)を遮光して室温で1時間反応させた。以降の操作は、すべて遮光下室温で行った。PBSTで10分間の洗浄を3回行った後、TOPRO-3(life Technologies社)で核染色を行い、PBSで10分間の洗浄を2回行い、Vectashield(Vector Laboratories Inc.社)を数滴落としてカバーガラスを載せ、共焦点レーザー顕微鏡で観察および撮影した。
血管透過性亢進により病態の悪化が観察されるのは腫瘍だけでなく、炎症性疾患や糖尿病性網膜症や加齢黄斑変性症等においても、新生血管の透過性亢進による病態の悪化が観察される。そこで、LPAが腫瘍血管のみならず、他の疾患モデルにおいても新生血管の血管透過性亢進の抑制に効果があるかどうかを解析した。
下肢の虚血モデルは、バージャー病や慢性閉塞性動脈硬化症の病態モデルとして利用されている。すなわち、マウス下肢の血管を切除すると、その血管支配領域に虚血が生じ、さらに手術手技により炎症がもたらされた結果、血管新生が誘導され、新生血管の透過性亢進に起因して下肢の筋肉部位に浮腫が生じる。LPAにこのような炎症や虚血による新生血管の透過性亢進を抑制する効果があるかどうかを検討した。
6週齢のC57BL/6NCrSlcマウス(♀、SLC社)を用いた。ペントバルビタール(共立製薬株式会社)麻酔下で、右下肢の鼠径部の皮膚を切開し、大腿静脈起始部を結紮した後、伏在動静脈末端を結紮し、さらにその他の分枝を剥離して本枝とともに切除した。切除後、皮膚切開部を滅菌済縫合糸(夏目制作所)で縫合し、麻酔から覚醒するまで37℃恒温器上に静置した。覚醒後、LPA群(n=3)にはLPA(3mg/kg/100μL)を、コントロール群(n=3)にはPBS(100μL)を、それぞれ腹腔内投与した。投与は3日連続で1日1回、合計3回行った。最終投与の24時間後に、大腿周囲の長さを測定した。
結果を図16に示した。LPA投与群ではコントロール群に比べ大腿周囲の長さが顕著に短くなり、浮腫が抑制されていることが判明した。
滲出性加齢黄斑変性は、脈絡膜新生血管を原因とする眼疾患であり先進国の失明原因第1位を占める。脈絡膜新生血管は、健常な血管と異なり血管透過性が亢進しており、血液成分が漏出するため網膜浮腫や網膜下液が生じて視機能障害を引き起こす。現在、脈絡膜新生血管の発生や増殖に関与するVEGFを阻害する薬剤が臨床応用されているが、未だに視機能を完全に回復させる治療法はなく、さらなる有効な治療法の開発が望まれている。そこで、LPAがこの脈絡膜新生血管の透過性亢進を抑制し得るかどうかを検討した。
8週齢のC57BL/6NCrSlcマウス(♀、SLC社)を用いた。ペントバルビタール(共立製薬株式会社)麻酔下でトロピカミド(参天製薬製)を点眼して散瞳し、細隙灯顕微鏡でマウス眼底を観察しながら眼底視神経乳頭周囲の網膜に両眼4か所ずつレーザーを照射し(Ultima 2000 SE)脈絡膜新生血管を誘発した。レーザー照射条件は、150mW、0.05秒、75μmとした。LPA群(n=3)にはLPA(3mg/kg/100μL)を、レーザー照射後5日目と6日目に腹腔内投与し、コントロール群(n=3)にはPBS(100μL)を、レーザー照射後5日目と6日目に腹腔内投与した。レーザー照射後7日目(LPA初回投与後48時間目)に、ペントバルビタール麻酔下でFITC標識70kDaデキストラン(SIGMA社)を尾静脈内に投与した。デキストランはPBSに溶解し、5mg/mLとなるように希釈して、その50μLを投与した。デキストラン投与の10分後に眼球を回収した。回収した眼球を4%パラホルムアルデヒド(PFA)/PBSに2時間浸漬した後、眼球から角膜、水晶体、網膜を取り除き、脈絡膜フラットマウントを作製した。その後、4℃のPBSを用いて洗浄した。30分毎に新しいPBSに交換しながら6時間洗浄を行った。続いて、ブロッキング溶液(5% normal goat serum/1%BSA/2%skim milk/PBS)を加えて室温で2時間ブロッキングを行った。1次抗体には、抗マウスCD31抗体であるPurified Hamster Anti-PECAM-1(MILLIPORE社:MAB1398Z)を用い、ブロッキング溶液で200倍希釈して切片上に滴下し、4℃で一晩反応させた。PBSTで30分間の洗浄を6回行い、さらにPBSで30分間洗浄を行った。2次抗体には、Alexa Fluor 488 Goat Anti-Hamster IgG(Jackson ImmunoResearch Labolatories社)を用い、ブロッキング溶液で400倍希釈して切片に滴下し、6時間遮光で反応させた。PBSTで30分間の洗浄を6回行い、Vectashildを数滴落とし、カバーガラスで封入した。共焦点レーザー顕微鏡にて観察し、写真撮影を行った。
結果を図17に示した。上段(CD31)は血管内皮細胞を抗CD31抗体で免疫染色した組織標本を観察した写真であり、血管内皮細胞が緑色蛍光に染色され、写真では白く描出されている。下段(Dextran)は新生血管から漏出したデキストランの蛍光を観察した写真である。上段に示したように、脈絡膜新生血管の大きさに関してLPA群とコントロール群との間に有意差は求められなかった。一方、下段に示したように、デキストランの漏出に関しては、LPA群は顕著にデキストランの漏出が抑制されており、LPAは血管透過性亢進を抑制する作用を有することが明らかとなった。この結果から、LPAは加齢黄斑変性症における血管透過性を抑制して病態を改善する効果を有することが判明した。
Claims (17)
- 血管形成異常を伴う疾患の治療薬の疾患部位への送達を促進するための薬剤送達促進剤であって、リゾリン脂質受容体を活性化する物質を有効成分として含有し、血管形成異常を伴う疾患の治療薬と組み合わせて使用することを特徴とする薬剤送達促進剤。
- リゾリン脂質受容体を活性化する物質がリゾリン脂質もしくはその前駆体、またはそれらの誘導体である請求項1に記載の薬剤送達促進剤。
- 血管形成異常を伴う疾患が固形がん、加齢黄斑変性症、関節リウマチ、乾癬、強皮症、全身性エリテマトーデス、血管炎症候群、血管ベーチェット、糖尿病性網膜症、糖尿病性腎症、動脈硬化症、慢性閉塞性動脈硬化症、バージャー病、肺線維症、脳梗塞、重症感染症または心不全である請求項1または2に記載の薬剤送達促進剤。
- 血管形成異常を伴う疾患が固形がん、加齢黄斑変性症、慢性閉塞性動脈硬化症またはバージャー病である請求項3に記載の薬剤送達促進剤。
- リゾリン脂質がリゾホスファチジン酸、リゾホスファチジルセリン、リゾホスファチジルコリン、リゾホスファチジルエタノールアミン、リゾホスファチジルイノシトール、リゾホスファチジルグリセロール、スフィンゴシン-1-リン酸、スフィンゴシルホスホリルコリンおよび血小板活性化因子(PAF)から選択される1種または2種以上である請求項1から4のいずれかに記載の薬剤送達促進剤。
- リゾリン脂質がリゾホスファチジン酸である請求項5に記載の薬剤送達促進剤。
- リゾリン脂質受容体を活性化する物質を有効成分として含有する血管形成異常を伴う疾患の治療用医薬組成物。
- リゾリン脂質受容体を活性化する物質がリゾリン脂質もしくはその前駆体、またはそれらの誘導体である請求項7に記載の医薬組成物。
- 腫瘍増殖抑制作用、がん転移抑制作用、腫瘍免疫亢進作用および血管透過性亢進抑制作用から選択される1種以上の作用を有する請求項8に記載の医薬組成物。
- 血管形成異常を伴う疾患治療用の他の薬剤と組み合わせて使用することを特徴とする請求項8または9に記載の医薬組成物。
- 血管形成異常を伴う疾患治療用の他の薬剤が、がん治療薬である請求項10に記載の医薬組成物。
- 組み合わせて使用する他の薬剤の血管形成異常を伴う疾患部位への送達を促進するための請求項10または11に記載の医薬組成物。
- 血管形成異常を伴う疾患が固形がん、加齢黄斑変性症、関節リウマチ、乾癬、強皮症、全身性エリテマトーデス、血管炎症候群、血管ベーチェット、糖尿病性網膜症、糖尿病性腎症、動脈硬化症、慢性閉塞性動脈硬化症、バージャー病、肺線維症、脳梗塞、重症感染症または心不全である請求項7~12のいずれかに記載の医薬組成物。
- 血管形成異常を伴う疾患が固形がん、加齢黄斑変性症、慢性閉塞性動脈硬化症またはバージャー病である請求項13に記載の医薬組成物。
- リゾリン脂質がリゾホスファチジン酸、リゾホスファチジルセリン、リゾホスファチジルコリン、リゾホスファチジルエタノールアミン、リゾホスファチジルイノシトール、リゾホスファチジルグリセロール、スフィンゴシン-1-リン酸、スフィンゴシルホスホリルコリンおよび血小板活性化因子(PAF)から選択される1種または2種以上である請求項7~14のいずれかに記載の医薬組成物。
- リゾリン脂質がリゾホスファチジン酸である請求項15に記載の医薬組成物。
- 血管形成異常を伴う疾患の治療薬のスクリーニング方法であって、疾患部位の血管内皮細胞に特異的に発現しているリゾリン脂質受容体を活性化する物質を選択することを特徴とする方法。
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US15/301,750 US20170112861A1 (en) | 2014-04-04 | 2015-04-03 | Drug delivery enhancer comprising substance for activating lysophospholipid receptors |
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US11033559B2 (en) | 2015-09-29 | 2021-06-15 | Osaka University | Leukocyte infiltration promoting agent and antitumor immunostimulatory agent |
WO2023145873A1 (ja) * | 2022-01-27 | 2023-08-03 | 国立大学法人東京大学 | リゾホスファチジルセリン類似体、及びリゾホスファチジルセリン類似体を含む線維症を治療又は予防するための医薬組成物 |
US11802135B2 (en) | 2021-11-03 | 2023-10-31 | Mitsubishi Tanabe Pharma Corporation | Lysophosphatidic acid derivative |
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CA3126203A1 (en) * | 2019-02-21 | 2020-08-27 | Universite Claude Bernard Lyon 1 | Structured molecular vectors for anti-inflammatory compounds and uses thereof |
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2015
- 2015-04-03 CA CA2946275A patent/CA2946275A1/en not_active Abandoned
- 2015-04-03 JP JP2016511651A patent/JP6139781B2/ja not_active Expired - Fee Related
- 2015-04-03 EP EP15772775.1A patent/EP3135297A4/en not_active Withdrawn
- 2015-04-03 AU AU2015242791A patent/AU2015242791B2/en not_active Ceased
- 2015-04-03 CN CN201580019069.1A patent/CN106170302B/zh not_active Expired - Fee Related
- 2015-04-03 US US15/301,750 patent/US20170112861A1/en not_active Abandoned
- 2015-04-03 WO PCT/JP2015/060666 patent/WO2015152412A1/ja active Application Filing
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2017
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2019
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11033559B2 (en) | 2015-09-29 | 2021-06-15 | Osaka University | Leukocyte infiltration promoting agent and antitumor immunostimulatory agent |
US11802135B2 (en) | 2021-11-03 | 2023-10-31 | Mitsubishi Tanabe Pharma Corporation | Lysophosphatidic acid derivative |
WO2023145873A1 (ja) * | 2022-01-27 | 2023-08-03 | 国立大学法人東京大学 | リゾホスファチジルセリン類似体、及びリゾホスファチジルセリン類似体を含む線維症を治療又は予防するための医薬組成物 |
Also Published As
Publication number | Publication date |
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US20170112861A1 (en) | 2017-04-27 |
JP6573329B2 (ja) | 2019-09-11 |
JP6139781B2 (ja) | 2017-05-31 |
CN106170302B (zh) | 2020-11-06 |
AU2015242791B2 (en) | 2017-08-17 |
CA2946275A1 (en) | 2015-10-08 |
AU2015242791A1 (en) | 2016-10-20 |
EP3135297A1 (en) | 2017-03-01 |
JP2020002143A (ja) | 2020-01-09 |
EP3135297A4 (en) | 2018-02-07 |
JPWO2015152412A1 (ja) | 2017-04-13 |
JP2017132804A (ja) | 2017-08-03 |
US20210205337A1 (en) | 2021-07-08 |
CN106170302A (zh) | 2016-11-30 |
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