WO2015005209A1 - アフラトキシン産生阻害剤、及びアフラトキシン汚染防除方法 - Google Patents

アフラトキシン産生阻害剤、及びアフラトキシン汚染防除方法 Download PDF

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WO2015005209A1
WO2015005209A1 PCT/JP2014/067775 JP2014067775W WO2015005209A1 WO 2015005209 A1 WO2015005209 A1 WO 2015005209A1 JP 2014067775 W JP2014067775 W JP 2014067775W WO 2015005209 A1 WO2015005209 A1 WO 2015005209A1
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aflatoxin
inhibitor
respiratory
production
production inhibitor
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PCT/JP2014/067775
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English (en)
French (fr)
Japanese (ja)
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庄平 作田
景子 ▲高▼城
ジヤン フェブリ プラボオ
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国立大学法人東京大学
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • A01N37/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
    • A—HUMAN NECESSITIES
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    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/24—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms
    • A—HUMAN NECESSITIES
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    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
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    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/742—Organic compounds containing oxygen
    • A23B2/75—Organic compounds containing oxygen with doubly-bound oxygen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/762—Organic compounds containing nitrogen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729—Organic compounds; Microorganisms; Enzymes
    • A23B2/771—Organic compounds containing hetero rings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10
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    • A23B7/154—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/16—Preserving with chemicals
    • A23B9/24—Preserving with chemicals in the form of liquids or solids
    • A23B9/26—Organic compounds; Microorganisms; Enzymes

Definitions

  • the present invention relates to an aflatoxin production inhibitor and an aflatoxin contamination control method.
  • Mold secondary metabolites include useful compounds, but there are many toxic compounds called mycotoxins.
  • mycotoxins Currently, contamination of crops with mycotoxins is a serious problem worldwide, and means for controlling mycotoxin contamination are required to stably obtain safe food.
  • Aflatoxin is known to have the strongest carcinogenicity and acute toxicity among known natural substances, and it is a compound that is not decomposed by ordinary cooking methods, so that its health damage is serious.
  • the regulation value for aflatoxin contamination of agricultural crops is as low as about 10 ppb.
  • this also increases the amount of damage caused by destroying crops contaminated with aflatoxins.
  • the loss due to aflatoxin contamination in the United States is tens of billions of yen per year.
  • the loss due to aflatoxin contamination in Asia is even greater, and is estimated to be over 100 billion yen per year.
  • an antifungal agent that inhibits the growth of aflatoxin-producing bacteria can be considered, for example, azoles such as cycloproconazole, strobilurins such as azoxystrobin, It is known that mycotoxins such as aflatoxins can be reduced before and after harvesting and from corn during storage by using one or more selected from the group consisting of other fungicides such as fludioxanil in combination. (See Patent Document 1). In the above-mentioned patent application, the situation in which mycotoxins such as aflatoxins are produced is considered in detail, and various countermeasures against them are studied. Ultimately, the growth of mycotoxin-producing bacteria can be suppressed.
  • Non-Patent Document 3 As a result of searching for substances that inhibit aflatoxin production, dichlorvos, an organophosphorus insecticide, and tricyclazole, which inhibits melanin biosynthesis enzymes, were found to have aflatoxin production inhibitory activity ( Non-Patent Document 3).
  • these compounds have not been put into practical use because they have weak aflatoxin production inhibitory activity, and there are problems with the safety of the compounds themselves, and there is a problem with selectivity of inhibitory activity.
  • an object of the present invention is to provide an aflatoxin production inhibitor that specifically and effectively inhibits the production of aflatoxin, has high safety, and is practical, and a method for controlling aflatoxin contamination.
  • the present inventors have intensively studied, and as a result, found that a respiratory inhibitor or a specific respiratory inhibitor has an excellent aflatoxin production inhibitory activity, and led to the completion of the present invention. .
  • the present invention is based on the above findings by the present inventors, and means for solving the above problems are as follows. That is, (1) An aflatoxin production inhibitor comprising a respiratory inhibitor, (2) The respiratory inhibitor is treated with a concentration at which the amount of aflatoxin producing bacteria is 50% or more of the amount of untreated cells, or the amount of aflatoxin producing bacteria is untreated.
  • the aflatoxin production inhibitor according to (1) which is a respiratory inhibitor that is 50% or more of the amount of bacterial cells in the ward
  • Respiratory inhibitors include rotenone, siccanin, atopenin A5, antimycin A, pyridaben, tolfenpyrad, flucripyr, flucripyrim selected from acequinocyl, bifenazate, mepronil, metminostrobin, pyribencarb, cyazofamid, and pyrimidifene. is there( )
  • an aflatoxin production inhibitor according to (4)
  • the respiratory inhibitor is an aflatoxin production inhibitor according to (1) or (2), which is fluacrylpyrim.
  • the respiratory inhibitor is at least one selected from rotenone, siccanin, atpenin A5, and antimycin A (1) or (2)
  • the aflatoxin production inhibitor described (6)
  • the aflatoxin production inhibitor according to any one of (1) to (5), a plant to which aflatoxin-producing bacteria may adhere, a fruit harvested from the plant, and / or the fruit at the time of storage A method for controlling aflatoxin contamination, characterized by comprising
  • the conventional problems can be solved, the object can be achieved, the production of aflatoxin can be specifically and effectively inhibited, a safe and practical aflatoxin production inhibitor, And an aflatoxin contamination control method can be provided.
  • the aflatoxin production inhibitor of the present invention contains at least a respiratory inhibitor, and may further contain other components as necessary.
  • the aflatoxin production inhibitor inhibits aflatoxin production, and the degree thereof is not particularly limited as long as the production amount of aflatoxin can be suppressed as compared with the non-treatment group of the inhibitor.
  • the aflatoxin production inhibitor of the present invention is preferably a respiratory inhibitor that is treated at a concentration such that the amount of aflatoxin-producing bacteria is 50% or more of the amount of cells in the untreated section, or an aflatoxin-producing bacterium It is preferable that the amount of microbial cells is a respiratory inhibitor that is 50% or more of the amount of microbial cells in the untreated section.
  • the respiratory inhibitor treated at a concentration that is 50% or more of the amount of cells in the untreated group means that the survival or growth of the aflatoxin-producing bacteria is less than 50% of the amount of cells in the untreated group, that is, the control group depending on the concentration
  • the amount of bacterial cells can be suppressed up to this point, but it means that the production of aflatoxin itself can be suppressed even when treated at a low concentration instead of such a high concentration.
  • the degree of suppressing the amount of cells is not particularly limited as long as the amount of cells in the treated group is 50% or more compared to the amount of cells in the untreated group, but is 70% or more compared to the untreated group. It is preferably 95% or more.
  • a respiratory inhibitor in which the amount of aflatoxin-producing bacteria is 50% or more of the amount of cells in the untreated group is different from the amount of cells in the untreated group, regardless of the treatment concentration. It means that the body weight is always 50% or more.
  • the degree of suppressing the amount of cells is not particularly limited as long as the amount of cells in the treated group is 50% or more compared to the amount of cells in the untreated group, but is 70% or more compared to the untreated group. Preferably, it is more preferably 95% or more, and it is usually preferable to use a respiratory inhibitor that is not active against bacteria.
  • Patent Document 1 azoxystrobin, fluoxastrobin, cresoxime methyl, picoxystrobin, pyraclostrobin, trifloxystrobin, and boscalid are described as respiratory inhibitors.
  • the present invention suppresses aflatoxin production by suppressing the survival or growth of aflatoxin-producing bacteria, and suppresses aflatoxin production without suppressing the survival or growth of the bacteria. Is clearly different from the technical idea.
  • the respiration inhibitor refers to an agent that inhibits respiration of microorganisms by inhibiting the mitochondrial electron transport system or losing a proton concentration gradient.
  • rotenone siccanin, atopenin A5, antimycin A, pyridaben, tolfenpyrad, fluacrylpyrim, acequinosyl, bifenazet, mepronil, metminostrobin, pyribencarb, cyazofamid, pyrimidifen, and fenproximate are survival of aflatoxin-producing bacteria Moreover, it is preferable at the point which can suppress the production of aflatoxin without suppressing proliferation.
  • the number of aflatoxin-producing bacteria is set to be untreated. Even when it is used at a concentration that suppresses only 50% or more, the production of aflatoxin itself can be suppressed as compared to the untreated section.
  • fluacrylpyrim and metminostrobin are more preferable, and fluacrylpyrim is particularly preferable in that it has excellent aflatoxin production inhibitory activity.
  • Rotenone, siccanin, atopenin A5, and antimycin A are derived from natural products and are preferable from the viewpoint of safety. Among these, siccanin, atopenin A5, and antimycin A are more preferable because of their excellent aflatoxin production inhibitory activity. preferable.
  • the respiratory inhibitor a commercially available product may be used, or a product produced by biosynthesis or chemical synthesis may be used. There is no restriction
  • the content of the respiratory inhibitor in the aflatoxin production inhibitor is not particularly limited and may be appropriately selected depending on the purpose.
  • the aflatoxin production inhibitor may be the respiratory inhibitor itself.
  • the other components in the aflatoxin production inhibitor are not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected according to the purpose.
  • any agrochemical, pharmaceutical component, pesticide, Examples include pharmaceutical adjuvants.
  • the content of the other components in the aflatoxin production inhibitor is not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected according to the purpose.
  • agrochemical component fungicides, bactericides, antiviral agents, plant resistance inducers, insecticides, acaricides, nematicides, insect growth regulators, insect attractants, herbicides, plants
  • growth regulators, synergists, safeners, bird repellents, fertilizers, and soil conditioners may be used alone or in combination of two or more.
  • the agrochemical adjuvant contains a carrier, a surfactant, and other adjuvants, and further contains other components as necessary.
  • the carrier is not particularly limited as long as it can be used for agriculture and horticulture, and can be appropriately selected according to the purpose. Examples thereof include a liquid carrier and a solid carrier. These may be used individually by 1 type and may use 2 or more types together.
  • liquid carrier examples include water; alcohols such as isopropyl alcohol and ethylene glycol; cyclohexanone; ketones such as methyl ethyl ketone; ethers such as propylene glycol monomethyl ether and diethylene glycol mono- n -butyl ether; fats such as kerosene and light oil.
  • Aromatic hydrocarbons such as xylene, trimethylbenzene, tetramethylbenzene, methylnaphthalene and solvent naphtha; amides such as N -methyl-2-pyrrolidone; esters such as glycerin esters of fatty acids; soybean oil And vegetable oils such as rapeseed oil.
  • solid carrier examples include animal and vegetable powders such as starch, activated carbon, soybean flour, wheat flour, wood flour, fish flour, and milk powder; talc, kaolin, bentonite, zeolite, diatomaceous earth, white carbon, clay, alumina, calcium carbonate, and chloride.
  • animal and vegetable powders such as starch, activated carbon, soybean flour, wheat flour, wood flour, fish flour, and milk powder
  • talc kaolin
  • bentonite zeolite
  • diatomaceous earth white carbon
  • clay alumina
  • calcium carbonate examples of the solid carrier
  • mineral powders such as potassium and ammonium sulfate.
  • a nonionic surfactant for example, anionic surfactant, a cationic surfactant, an amphoteric surfactant Etc.
  • anionic surfactant for example, anionic surfactant, a cationic surfactant, an amphoteric surfactant Etc.
  • anionic surfactant for example, anionic surfactant, a cationic surfactant, an amphoteric surfactant Etc.
  • nonionic surfactant examples include polyoxyethylene alkyl ether, polyoxyethylene alkyl aryl ether, polyoxyethylene styryl phenyl ether, polyoxyethylene alkyl ester, polyoxyethylene sorbitan alkylate, polyoxyethylene phenyl ether.
  • examples thereof include polymers, polyoxyethylene alkylene aryl phenyl ethers, polyoxyethylene alkylene glycols, and polyoxyethylene polyoxypropylene block polymers.
  • anionic surfactant examples include lignin sulfonate, alkylaryl sulfonate, dialkyl sulfosuccinate, polyoxyethylene alkyl aryl ether sulfate, alkyl naphthalene sulfonate, polyoxyethylene styryl phenyl ether sulfate, and the like. Is mentioned.
  • Examples of the cationic surfactant include alkylamine salts.
  • Examples of the amphoteric surfactant include quaternary ammonium salt alkylbetaines and amine oxides.
  • the other adjuvant is not particularly limited and may be appropriately selected depending on the intended purpose.
  • a binder a thickener, a sticking agent, an antiseptic and fungicide, a solvent, and stabilization of an agrochemical active ingredient.
  • Agents antioxidants, ultraviolet light inhibitors, crystal precipitation inhibitors, antifoaming agents, physical property improvers, colorants and the like.
  • the binder, thickener, and fixing agent are not particularly limited and may be appropriately selected depending on the intended purpose.
  • dextrin cellulose, methylcellulose, ethylcellulose, carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, Hydroxypropyl methylcellulose, carboxymethyl starch, pullulan, sodium alginate, ammonium alginate, propylene glycol ester alginate, guar gum, locust bean gum, gum arabic, xanthan gum, gelatin, casein, polyvinyl alcohol, polyethylene oxide, polyethylene glycol, ethylene / propylene block polymer , Sodium polyacrylate, polyvinylpyrrolidone and the like.
  • the dosage form of the aflatoxin production inhibitor is not particularly limited and may be appropriately selected depending on the intended purpose.
  • a liquid agent is preferable.
  • the aflatoxin production inhibitors include other fungicides (fungicides, bactericides, antiviral agents, plant resistance inducers, etc.), insecticides, acaricides, nematicides, insect growth regulators, insects It can also be used in combination or in combination with attractants, herbicides, plant growth regulators, synergists, safeners, bird repellents, fertilizers, soil conditioners and the like.
  • the aflatoxin production inhibitor has an excellent aflatoxin production inhibitory activity, it can be suitably used in a method for controlling aflatoxin contamination.
  • the method for controlling aflatoxin contamination according to the present invention comprises a plant having a possibility that an aflatoxin-producing bacterium adheres thereto, a fruit harvested from the plant, and / or the fruit at the time of storage.
  • a method for controlling aflatoxin contamination characterized by comprising
  • the plant to which the aflatoxin-producing bacteria may adhere means a plant to which the aflatoxin-producing bacteria may adhere and infect as a host, and the plant is already in an uninfected state even if it is not yet infected. It may be in an infected state, may be in an already infected state and is not yet ill, or may be in a state after being ill.
  • the fruit which is still a harvest may be before or after fruiting. Further, it may be any case where the inhibitor is treated on the fruit before harvesting and / or on the fruit after harvesting. Further, when the fruit is stored for a short period or a long period after harvesting, it may be any case where the fruit is processed at the initial stage of storage, during storage, and / or before shipment.
  • the treatment method is not particularly limited as long as the production inhibitor can be brought into contact with the object to be controlled.
  • the aflatoxin production inhibitor is spread as it is or diluted with water or the like (for example, Spraying, misting, atomizing, dusting, dusting, water surface application, box application, etc.), soil application (eg, mixing, irrigation, etc.), surface application (eg, application, dressing, coating, etc.) And a dipping method.
  • a target object which processes an aflatoxin production inhibitor there is no restriction
  • a plant body, agricultural products, etc. are mentioned.
  • the agricultural crops include grains such as corn, rice, buckwheat and pearl barley; nuts such as peanut, pistachio nut and brazil nut; spices such as nutmeg, chili and paprika; beans such as coffee beans; sesame seeds; And so on.
  • the application amount of the aflatoxin production inhibitor is not particularly limited, and includes the form of the preparation, the target disease or crop type, the degree of damage caused by the disease, the application location, the application method, the application timing, the drugs to be mixed or used together, fertilizer, etc. Can be appropriately selected according to various conditions such as the type, amount used, and weather.
  • the application concentration of the aflatoxin production inhibitor is not particularly limited and may be appropriately selected depending on the intended purpose. It is preferable to apply the concentration of the respiratory inhibitor to 0.001 mM or more. It is more preferable to apply so that it may become 0.01 mM or more. When the application concentration is less than 0.01 mM, production of aflatoxins may not be sufficiently inhibited. In particular, it is preferable to treat at a concentration such that the amount of aflatoxin-producing bacteria is 50% or more, preferably 70% or more, and more preferably 95% or more compared to the untreated group.
  • Respiration inhibitors shown in Table 4 were placed in each well of a 24-well microplate so that the final concentrations were 0 mM (control) and 0.2 mM, respectively.
  • Potato dextrose liquid medium manufactured by Difco was added so that the liquid volume in each well was 1 mL in total.
  • 10 ⁇ L / well of the spore suspension A. parasiticus NRRL 2999 was inoculated and statically cultured at 27.5 ° C. for 3 days.
  • the culture solution in each well was centrifuged to separate the cells and the culture supernatant.
  • the cells were washed with 5 mL of distilled water, transferred to a 1.5 mL microtube, and lyophilized. After the lyophilization, the mass of the microtube containing the microbial cells was measured, and the mass obtained by subtracting the mass of the empty microtube was taken as the microbial mass.
  • Aflatoxin production inhibition rate (%) ⁇ (XY) / X ⁇ ⁇ 100
  • X Aflatoxin production ( ⁇ g / mL) in the case of control (no respiratory inhibitor added)
  • Y Aflatoxin production amount ( ⁇ g / mL) when a respiratory inhibitor is added
  • Aflatoxin production amount A. parasiticus is a aflatoxins production can be confirmed in the culture of NRRL 2999, was calculated using the amount of the sum of aflatoxin B 1 and aflatoxin G 1.
  • each respiratory inhibitor has an aflatoxin production inhibitory activity.
  • Respiratory inhibitors other than Cresoxime Methyl had little effect on mold growth and were confirmed to selectively inhibit aflatoxin production.
  • fluacrylpyrim had the highest selectivity and strong aflatoxin production inhibitory activity compared to other drugs that showed an effect at 0.001 mM.

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