WO2014063587A1 - 一类氟取代的环状胺类化合物及其制备方法、药物组合物和用途 - Google Patents
一类氟取代的环状胺类化合物及其制备方法、药物组合物和用途 Download PDFInfo
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- WO2014063587A1 WO2014063587A1 PCT/CN2013/085356 CN2013085356W WO2014063587A1 WO 2014063587 A1 WO2014063587 A1 WO 2014063587A1 CN 2013085356 W CN2013085356 W CN 2013085356W WO 2014063587 A1 WO2014063587 A1 WO 2014063587A1
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- 0 C*C(C1)N(C*)C(*)CC1(CC(Cc1ccccc11)C1=O)F Chemical compound C*C(C1)N(C*)C(*)CC1(CC(Cc1ccccc11)C1=O)F 0.000 description 1
- NXKGRFBZXYZCLN-UHFFFAOYSA-N CC(C)(C)OC(N(CC1)CCC1(CC(Cc(c1c2)cc(OC)c2OC)C1=O)F)=O Chemical compound CC(C)(C)OC(N(CC1)CCC1(CC(Cc(c1c2)cc(OC)c2OC)C1=O)F)=O NXKGRFBZXYZCLN-UHFFFAOYSA-N 0.000 description 1
- AXDQDBJHAKENND-UHFFFAOYSA-N Oc1ccc(CC(CC2(CCNCC2)F)C2=O)c2c1 Chemical compound Oc1ccc(CC(CC2(CCNCC2)F)C2=O)c2c1 AXDQDBJHAKENND-UHFFFAOYSA-N 0.000 description 1
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- C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
- C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
- C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
- C07D211/30—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by doubly bound oxygen or sulfur atoms or by two oxygen or sulfur atoms singly bound to the same carbon atom
- C07D211/32—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by doubly bound oxygen or sulfur atoms or by two oxygen or sulfur atoms singly bound to the same carbon atom by oxygen atoms
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- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
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- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
- A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
- A61K31/4525—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with oxygen as a ring hetero atom
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- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/46—8-Azabicyclo [3.2.1] octane; Derivatives thereof, e.g. atropine, cocaine
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- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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- C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
- C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
- C07D211/08—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms
- C07D211/18—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms
- C07D211/34—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hydrocarbon or substituted hydrocarbon radicals directly attached to ring carbon atoms with substituted hydrocarbon radicals attached to ring carbon atoms with hydrocarbon radicals, substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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- C07D—HETEROCYCLIC COMPOUNDS
- C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
- C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
- C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D211/38—Halogen atoms or nitro radicals
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- C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
- C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
- C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D211/60—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/06—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D451/00—Heterocyclic compounds containing 8-azabicyclo [3.2.1] octane, 9-azabicyclo [3.3.1] nonane, or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane or granatane alkaloids, scopolamine; Cyclic acetals thereof
- C07D451/02—Heterocyclic compounds containing 8-azabicyclo [3.2.1] octane, 9-azabicyclo [3.3.1] nonane, or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane or granatane alkaloids, scopolamine; Cyclic acetals thereof containing not further condensed 8-azabicyclo [3.2.1] octane or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane; Cyclic acetals thereof
- C07D451/04—Heterocyclic compounds containing 8-azabicyclo [3.2.1] octane, 9-azabicyclo [3.3.1] nonane, or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane or granatane alkaloids, scopolamine; Cyclic acetals thereof containing not further condensed 8-azabicyclo [3.2.1] octane or 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring systems, e.g. tropane; Cyclic acetals thereof with hetero atoms directly attached in position 3 of the 8-azabicyclo [3.2.1] octane or in position 7 of the 3-oxa-9-azatricyclo [3.3.1.0<2,4>] nonane ring system
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- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/08—Bridged systems
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- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/10—Spiro-condensed systems
- C07D491/107—Spiro-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
Definitions
- Fluorine-substituted cyclic amine compound preparation method thereof, pharmaceutical composition and use thereof
- the present invention relates to the field of medicinal chemistry and pharmacotherapeutics, and in particular to a class of fluoro substituted cyclic amine compounds, processes for their preparation, pharmaceutical compositions containing such compounds, and as acetylcholinesterase inhibitors, particularly for preparation Use of a medicament for the treatment of Alzheimer's disease, Parkinson's disease, epilepsy, schizophrenia.
- BACKGROUND OF THE INVENTION With the rapid development of the aging society, the health status of the elderly has received increasing attention. Among the many diseases that threaten the health of the elderly, Alzheimer's disease (AD), also known as senile dementia, is the most common cause of dementia in the elderly. AD is a progressive development of fatal neurodegenerative diseases.
- AD Alzheimer's disease
- AD Alzheimer's disease
- AD has become the fourth killer after cardiovascular, tumor and stroke. Therefore, the study of drugs for the treatment of AD has become one of the hotspots of new drug research.
- the market for anti-senile dementia drugs in the top seven pharmaceutical markets in the world has reached $6.41 billion in 2007, an increase of 24.18% from the previous year.
- the market for anti-senile dementia drugs increased by 12.49% over the same period of the previous year, reaching a market size of 7.211 billion US dollars.
- the average annual growth rate in the past three years was about 23%, far higher than the average annual global pharmaceutical market. 5% to 6% increase.
- acetylcholinesterase inhibitor (AchEI) is the most effective and clinically applicable in the clinical treatment of senile dementia.
- Cholinesterase is a key enzyme in biological nerve conduction. Cholinesterase is divided into acetylcholinesterase (AChE) and butyrylcholinesterase depending on the specificity of its catalytic substrate. Acetylcholinesterase catalyzes the cleavage reaction of acetylcholine, leading to the loss of acetylcholine and the failure of nerve signaling, which leads to decreased cognitive function, loss of memory, and clinical manifestations of Alzheimer's disease.
- AChE acetylcholinesterase
- butyrylcholinesterase depending on the specificity of its catalytic substrate.
- Acetylcholinesterase catalyzes the cleavage reaction of acetylcholine, leading to the loss of acetylcholine and the failure of nerve signaling, which leads to decreased cognitive function, loss of memory, and clinical manifestations of Alzheimer's disease.
- Acetylcholinesterase inhibitors can inhibit the activity of AChE, delay the hydrolysis of acetylcholine, increase the level of acetylcholine in the synaptic cleft, and ensure the normal transmission of the signal of God, thereby exerting a therapeutic effect on Alzheimer's disease.
- Donepezil hydrochloride (E2020) disclosed in EP 0 296 560 A2 is a second-generation acetylcholinesterase inhibitor whose therapeutic effect reversibly inhibits acetylcholine hydrolysis caused by acetylcholinesterase (AChE) and increases the acetylcholine content at the receptor site.
- E2020 was launched in the United States in 1997. It is the second drug approved by the US FDA for the treatment of mild and moderate Alzheimer's disease. It is currently in China. More than 50 countries and regions have been approved to market, and it is currently a safe and effective drug for the treatment of Alzheimer's disease.
- E2020 has become the drug of choice for the treatment of mild to moderate Alzheimer's disease.
- the specific affinity of donepezil hydrochloride for acetylcholinesterase is 1250 times stronger than that of butyrylcholinesterase, which can significantly inhibit cholinesterase in brain tissue and butyrylcholinesterase mainly exists outside the central nervous system, so E2020 It has no effect on the peripheral heart (myocardium) or the small intestine (smooth muscle), and has few side effects. Compared with Tallin, E2020 has better effects, higher selectivity and less central nervous toxicity. As a result, E2020 became the first line of clinical treatment for Alzheimer's disease, with global sales reaching $3.4 billion in 2010.
- One object of the present invention is to provide a fluorine-substituted cyclic amine compound of the formula I, a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a mixture of them.
- Another object of the present invention is to provide a process for producing a fluorine-substituted cyclic amine compound represented by the above formula I.
- It is still another object of the present invention to provide a pharmaceutical composition comprising a therapeutically effective amount selected from the above formula I
- a pharmaceutical composition comprising a therapeutically effective amount selected from the above formula I
- a fluorine-substituted cyclic amine compound a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a mixture thereof.
- Still another object of the present invention is to provide an acetylcholinesterase inhibitor comprising a fluorine-substituted cyclic amine compound selected from the above formula I, a pharmaceutically acceptable salt thereof, a racemate, and R- One or more of an isomer, an S-isomer, or a mixture thereof.
- Still another object of the present invention is to provide a fluorine-substituted cyclic amine compound represented by the above formula I, a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a
- a fluorine-substituted cyclic amine compound represented by the above formula I a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a
- a neurological disease associated with acetylcholinesterase such as Alzheimer's disease, Parkinson's disease, epilepsy, schizophrenia and the like.
- the patient is administered a fluorine-substituted cyclic amine compound represented by the above formula I, a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a mixture thereof.
- a fluorine-substituted cyclic amine compound represented by the above formula I a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a mixture thereof.
- the present invention provides a fluorine-substituted cyclic amine compound having the structure represented by the following formula I, and a racemate, an R-isomer, an S-isomer, a pharmaceutically acceptable salt thereof Or a mixture of them:
- n is an integer of 0 to 3; wherein m is preferably 0, 1, or 2.
- n is an integer of 0 to 3; wherein n is preferably 0, 1, or 2.
- X is (CH 2 ) P , CO or S0 2 , wherein p is an integer of 0 to 3; X is preferably (CH 2 ) P or CO, and p is preferably 1 or 2.
- it is a substituted or unsubstituted C 3 -C 8 cycloalkyl group or a substituted or unsubstituted C 6 -C 12 aryl group; the substituent in the above is selected from halogen, dC 6 alkyl, halogen substituted dC 6 alkyl, dC 6 alkoxy, dC 6 alkoxycarbonyl group, substituted with a prime ⁇ dC 6 alkoxy, C 2 -C 6 alkenyl, C 2 -C 6 block basis, 8 cycloalkyl, The same or different 1 to 5 substituents of a cyano group, a nitro group, an amino group, a hydroxyl group, a hydroxymethyl group, a carboxyl group, a decyl group, a sulfonyl group, a phenyl group, a naphthyl group and a 3-12 membered heterocyclic group; Two adjacent substituents on the
- it is a C 3 -C 8 cycloalkyl group, a substituted or unsubstituted phenyl group or a substituted or unsubstituted naphthyl group; the substituent in the above is selected from halogen, dC 6 alkyl group, dC 6 alkane An oxy group, a dC 6 alkoxy group substituted with a halogen, a dC 6 alkoxycarbonyl group, a C 2 -C 6 alkenyl group, a C 2 -C 6 block group, a cyano group, a nitro group, an amino group, a hydroxyl group, a hydroxymethyl group, 1 to 5 identical or different substituents in trifluoromethyl, trifluoromethoxy, carboxy, decyl, sulfonyl and phenyl, or in said benzene Most preferably, it is a cyclobutyl group, a cyclopentyl
- the base is composed of carbon atoms on its adjacent benzene ring ;
- R 2 and R 3 are each independently selected from the group consisting of hydrogen, carboxyl, dC 4 alkoxycarbonyl and dC 4 alkyl; or R 2 and R 3 are taken together to form a dC 4 alkylene;
- R 2 and R 3 are each independently selected from the group consisting of hydrogen, carboxyl, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, methyl, ethyl, propyl, isopropyl, butyl and 2- In the methyl propyl group; or R 2 and R 3 are joined together to form a sub Methyl, ethylene or propylene;
- R4 is selected from the group consisting of hydrogen, halogen, CC 6 alkyl, dC 6 alkyl substituted with halogen, dC 6 alkoxy, halogen substituted CC 6 alkoxy, C 2 -C 6 alkenyl, C 2 -C 6 Block group, cyano group, nitro group, amino group, hydroxyl group, hydroxymethyl group, carboxyl group, sulfhydryl group, sulfonyl group, -0[(CH 2 ) q O] r R 5 , phenyl group and 3-12 membered heterocyclic group 1 to 4 identical or different substituents; wherein the heterocyclic group contains 1 to 3 hetero atoms selected from the group consisting of oxygen, sulfur and nitrogen; and R 5 is selected from the group consisting of hydrogen, halogen, dC 6 alkyl, halogen dC 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 block and hydroxymethyl;
- R4 is selected from hydrogen, halo, dC 6 alkyl, substituted with halogen dC 6 alkyl, dC 6 alkoxy, halogen-substituted dC 6 alkoxy, C 2 -C 6 alkenyl, C 2 -C 6 block, cyano, nitro, amino, hydroxy, carboxy and 1 to 3 identical or different substituents in -0[(CH 2 ) q O] r R 5 ;
- R 5 is selected from hydrogen, Halogen, dC 6 alkyl and halogen substituted dC 6 alkyl; q is 1, 2 or 3; r is 1, 2 or 3;
- R4 is selected from hydrogen, halo, dC 6 alkyl, dC 6 alkoxy, halogen substituted dC 6 alkoxy, hydroxyl, and -0 [(CH 2) q C ⁇ R 5 1 ⁇ 2 identical or different substituents; R 5 is selected from dC 6 alkyl and halogen substituted dC 6 alkyl; q is 1, 2 or 3; r is 1, 2 or 3.
- the halogen is F, Cl, Br or I.
- the term "dC 6 alkyl” means a straight or branched alkyl group having 1 to 6 carbon atoms, and includes, without limitation, methyl, ethyl, propyl, isopropyl, butyl. , isobutyl, sec-butyl, tert-butyl, pentyl and hexyl, etc.; preferably ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl and tert-butyl.
- the term "dC 6 alkoxy” means a straight or branched alkoxy group having 1 to 6 carbon atoms, and includes, without limitation, a methoxy group, an ethoxy group, a propoxy group, and a different Propyloxy and butoxy and the like.
- C 2 -C 6 alkenyl means a straight or branched alkenyl group having 2 to 6 carbon atoms and containing a double bond, and includes, without limitation, a vinyl group, a propenyl group, and a butyl group. Alkenyl, isobutenyl, pentenyl, hexenyl and the like.
- C 2 -C 6 block group means a straight or branched chain group having one triple bond having 2 to 6 carbon atoms, and includes, without limitation, an ethyl group and a propyl group. , butyl group, isobutyl block, pentyl group and hexyl group.
- C 3 -C 1Q cycloalkyl means a cyclic alkyl group having 3 to 10 carbon atoms in the ring, and includes, without limitation, a cyclopropyl group, a cyclobutyl group, a cyclopentyl group. , cyclohexyl, cycloheptyl, cyclooctyl and cyclodecyl.
- C 3 -C 8 cycloalkyl “C 3 -C 7 cycloalkyl”
- C 3 -C 6 cycloalkyl have similar meanings.
- C 3 -C 1Q cycloalkenyl means a cyclic alkenyl group having 3 to 10 carbon atoms in the ring, and includes, without limitation, a cyclopropenyl group, a cyclobutenyl group, a cyclopentane group. Alkenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl and cyclo Alkene and the like.
- c 3 -c 7 cycloalkenyl has a similar meaning.
- C 6 -C 12 aryl means an aromatic ring group having 6 to 12 carbon atoms which does not contain a hetero atom in the ring, such as a phenyl group, a naphthyl group or the like.
- C 6 -C 1Q aryl has a similar meaning.
- 3-12 membered heterocyclic group means a saturated or unsaturated 3-12 membered ring group having 1 to 3 hetero atoms selected from the group consisting of oxygen, sulfur and nitrogen, for example, Dioxolyl and the like.
- 3-7 membered heterocyclic group has a similar meaning.
- the fluorine-substituted cyclic amine compound of the structure represented by the above formula I is a fluorine-substituted cyclic amine having the structure represented by the following formula II.
- R 6 represents 1 to 5 substituents, each of which is independently selected from the group consisting of g H, halogen, nitro, cyano, dC 6 alkyl, halogen-substituted dC 6 alkyl , dC 6 alkoxy, halogen-substituted CC 6 alkoxy, phenyl and dC 6 alkoxycarbonyl, or two adjacent benzene rings adjacent thereto
- it is or a C 3 -C 7 cycloalkyl group, which represents 1 to 5 substituents, each of which is independently selected from the group consisting of H, halogen, nitro, cyano, dC 4 alkyl, halogen-substituted dC 4 alkyl, dC 4 alkoxy, halogen substituted dC 4 alkoxy, phenyl and dC 4 alkoxycarbonyl, or two adjacent adjacent thereto More preferably, it is 1, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl, which represents 1 to 5 substituents, each of which is independently selected from H, halogen, nitro, cyano, - F, -Br, trifluoromethyl, trifluoroethyl, trifluoropropyl, methyl, ethyl, propyl, isopropyl, butyl, 2-methylpropyl, pheny
- R 2 and R 3 are each independently selected from the group consisting of hydrogen, carboxyl, CC 4 alkoxycarbonyl and dC 4 alkyl; or R 2 and R 3 are taken together to form a dC 4 alkylene group;
- R 2 and R 3 are each independently selected from the group consisting of hydrogen, carboxyl, methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, methyl, ethyl, propyl, isopropyl, butyl and 2- In the methyl propyl group; or R 2 and R 3 are joined together to form a methylene group, an ethylene group or a propylene group.
- R4 represents one to four substituents, the substituents are each independently selected from hydrogen, halo, dC 6 alkyl, substituted with halogen dC 6 alkyl, dC 6 alkoxy, prime ⁇ alkoxy substituted dC 6 a C 2 -C 6 alkenyl group, a C 2 -C 6 block group, a cyano group, a nitro group, an amino group, a hydroxyl group, a carboxyl group and -0[(CH 2 ) q O] r R 5 ; wherein R 5 is selected from Hydrogen, halogen, dC 6 alkyl and halogen substituted CC 6 alkyl; q is 1, 2 or 3; r is 1, 2 or 3;
- R4 represents 1-2 substituents, each substituent being independently selected from hydrogen, halo, dC 6 alkyl, dC 6 alkoxy, halogen substituted dC 6 alkoxy, hydroxy, and -0 [(CH 2 ) q C ⁇ R 5 ; wherein R 5 is selected from dC 6 alkyl and halogen-substituted CC 6 alkyl; q is 1, 2 or 3; r is 1, 2 or 3. Number Chinese name structure
- the compounds of the invention have asymmetric centers, chiral axes and chiral planes and may exist in the form of racemates, R-isomers or S-isomers. Those skilled in the art will be able to resolve the R-isomer and/or the S-isomer from the racemate by conventional techniques.
- the invention provides a pharmaceutically acceptable salt of a compound of formula I, in particular a compound of formula I, which is reacted with an inorganic or organic acid to form a conventional pharmaceutically acceptable salt.
- conventional pharmaceutically acceptable salts can be prepared by reacting a compound of formula I with an inorganic or organic acid, including hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, aminosulfonic acid, phosphoric acid, and the like, and Organic acids include citric acid, tartaric acid, lactic acid, pyruvic acid, acetic acid, benzenesulfonic acid, p-toluenesulfonic acid, methanesulfonic acid, naphthalenesulfonic acid, ethanesulfonic acid, naphthalene disulfonic acid, maleic acid, malic acid, and acrylic acid.
- Acid fumaric acid, succinic acid, propionic acid, oxalic acid, trifluoroacetic acid, stearic acid, pamoic acid, hydroxymaleic acid, phenylacetic acid, benzoic acid, salicylic acid, glutamic acid, ascorbic acid, p-aminobenzene a sulfonic acid, 2-acetoxybenzoic acid, and isethionate; or a sodium salt, a potassium salt, a calcium salt, an aluminum salt or an ammonium salt formed from a compound of the formula I and an inorganic base; or a compound of the formula I and an organic base A methylamine salt, an ethylamine salt or an ethanolamine salt is formed.
- Another aspect of the present invention provides a process for the preparation of a compound represented by the formula I, which is carried out according to the following Scheme 1 or Scheme 2.
- R 2 , R 3 , R 4 , X, m and n have the same definitions as in the above formula I.
- Step a Add dimethyl sulfoxide to DMSO, heat with stirring, add NaH, continue stirring, then cool, then add trimethylsulfoxonium iodide; then add compound 1 to obtain epoxy compound 2; heating temperature range is 60 ⁇ 100 o C.
- Step b dissolving the intermediate 2 in an organic solvent, cooling to a temperature of 10 ° C to 40 ° C, adding 1-10 equivalents of a hydrogen fluoride pyridine solution, continuing the reaction until the disappearance of the starting material, and separating and purifying the intermediate 3;
- the organic solvent may be tetrahydrofuran, diethyl ether, dimethylformamide, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, dioxane, ethanol, methanol, ethyl acetate, dichloromethane or a mixture thereof.
- Step c dissolving the intermediate 3 in an organic solvent, adding an oxidizing agent to oxidize the alcoholic hydroxyl group to an aldehyde group, to obtain an intermediate 4;
- the organic solvent may be tetrahydrofuran, diethyl ether, dimethylformamide, ethylene glycol dimethyl ether, Ethylene glycol diethyl ether, dioxane, ethanol, methanol, ethyl acetate, dichloromethane or a mixture thereof;
- the oxidizing agent may be selected from the group consisting of PCC, PDC, Dess-Martin oxidizing agent, Swem oxidizing agent, 3 ⁇ 40 2 , high manganese In potassium acid and manganese dioxide.
- Step d dissolving the intermediate 4 in an organic solvent, adding the compound 5, and adding a strong base to the disappearance of the raw material, and separating and purifying the intermediate 6;
- the organic solvent may be tetrahydrofuran, diethyl ether, dimethylformamide, or Diol dimethyl ether, ethylene glycol diethyl ether, dioxane, ethanol, methanol, ethyl acetate, dichloromethane or a mixture thereof;
- the strong base is NaOH, KOH, sodium ethoxide or sodium methoxide.
- Step e dissolving the intermediate 6 in an organic solvent, adding palladium carbon, and reducing by hydrogen to obtain the intermediate 7;
- the organic solvent may be tetrahydrofuran, diethyl ether, dimethylformamide, ethylene glycol dimethyl ether, Ethylene glycol diethyl ether, dioxane, ethanol, methanol or ethyl acetate, dichloromethane.
- Step f The intermediate 7 is dissolved in an organic solvent, and an organic solvent of trifluoroacetic acid (TFA) or hydrochloric acid (HC1) is added to remove the Boc protecting group to obtain an intermediate 8;
- the organic solvent may be tetrahydrofuran or diethyl ether.
- Step g The intermediate 8 is dissolved in an organic solvent, and the compound 9 is added, and a certain amount of a base is added thereto, and the mixture is stirred until the starting material disappears to obtain a final product;
- the organic solvent may be tetrahydrofuran, diethyl ether, dimethylformamide or ethylene.
- the base may be sodium acetate, NaOH, KOH, sodium ethoxide, sodium methoxide, carbonic acid Sodium, potassium carbonate, triethylamine or diisopropylamine.
- Step b' preparing the intermediate 2 by reducing the compound with a reducing agent;
- the reducing agent may be selected from the group consisting of sodium borohydride, potassium borohydride, lithium borohydride, lithium aluminum hydride (LiAlH 4 ).
- Step c-g The specific operation is the same as the above scheme 1.
- Another aspect of the invention provides a pharmaceutical composition comprising a therapeutically effective amount of a compound selected from the above formula (I), a pharmaceutically acceptable salt thereof, an enantiomer, a diastereomer thereof Or one or more of the racemates, and optionally one or more pharmaceutically acceptable carriers, excipients, adjuvants, adjuvants, and/or diluents.
- the excipient is, for example, an odorant, a flavoring agent, a sweetener or the like.
- the pharmaceutical composition provided by the present invention preferably contains the active ingredient in a weight ratio of 1-99%, preferably in a ratio of 65 wt% to 99 wt% of the total weight of the compound of the formula I, and the remainder is pharmaceutically acceptable.
- the compounds and pharmaceutical compositions provided herein may be in a variety of forms, such as tablets, capsules, powders, syrups, solutions, suspensions, and aerosols, and may be presented in a suitable solid or liquid carrier or diluent. Neutralizes a suitable sterilizing device for injection or drip.
- the unit dosage of the formulation of the formulation comprises 0.05-200 mg of the compound of the formula I, preferably, the unit dosage of the formulation comprises O.lmg-lOOmg of the compound of formula I.
- the compounds and pharmaceutical compositions of the present invention can be used clinically in mammals, including humans and animals, and can be administered by oral, nasal, dermal, pulmonary or gastrointestinal routes. Most preferably oral. Most preferably, the daily dose is 0.01-200 mg/kg body weight, taken once, or 0.01-100 mg/kg body weight. Regardless of the method of administration, the individual's optimal dose should be based on the specific treatment. It is usually starting with a small dose and gradually increasing the dose until the most suitable dose is found.
- a further aspect of the invention provides an acetylcholinesterase inhibitor comprising a compound selected from the above formula I, a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-iso One or more of the constructs or mixtures thereof, and optionally one or more pharmaceutically acceptable carriers, excipients, adjuvants, adjuvants, and/or diluents.
- the compounds and compositions of the present invention are useful for the treatment and prevention of neurological diseases associated with acetylcholinesterase, including, but not limited to, senile dementia, Parkinson's syndrome, epilepsy, schizophrenia and the like.
- a further aspect of the invention provides a compound of the above formula I, a pharmaceutically acceptable salt, a racemate, an R-isomer, an S-isomer or a mixture thereof, for use in the preparation of Use in the treatment of diseases associated with acetylcholinesterase-related neurological diseases such as Alzheimer's disease, Parkinson's disease, epilepsy, schizophrenia and the like.
- Yet another aspect of the present invention provides a method of treating a nervous system disease associated with acetylcholinesterase, such as Alzheimer's disease, Parkinson's disease, epilepsy, schizophrenia, etc., which includes the need for the treatment
- the patient is administered one or more selected from the group consisting of a compound of the above formula I, a pharmaceutically acceptable salt thereof, a racemate, an R-isomer, an S-isomer or a mixture thereof.
- Specific Embodiments The present invention will be further exemplified in the following examples. These examples are for illustrative purposes only and are not intended to limit the invention in any way.
- the starting materials used in the present invention are commercially available unless otherwise specified.
- the silica gel was filtered, and the filtrate was dried to give a colorless liquid product of about 1.3 g, which was 2-((4-fluoro-1-tert-butoxycarbonyl-substituted piperazin-4-yl)methyl)-5,6-dimethyl Oxy-2,3-dihydro-1H-1-indanone.
- the 4-nitrobenzyl bromide was replaced with 2,3,4,5,6-pentafluorobenzyl bromide, and the remaining desired starting materials, reagents and preparation methods were the same as those in Example 1, to obtain the target product DC28 in a yield of 80%.
- Example 24 2- ⁇ 4-Fluoro-[1-(3-methylbenzyl)]piperidin-4-methyl ⁇ -5,6-dimethoxy-2,3-dihydro-1-indole Preparation of ketone Replacing 4-nitrobenzyl bromide with 3-methylbenzyl bromide, the remaining starting materials, reagents and preparation methods are the same as in Example 1, to obtain the target product DC29, yield 80% 1H NMR (CDC1 3 , 400 MHz) ⁇ 7.19-7.24 (m, 3H), 7.17 (s, 1H), 7.12 (m, 1H), 6.86 (s, 1H), 3.96(s, 3H), 3.90(s, 3H), 3.66(s, 2H) , 3.31-3.37(m, 1H), 2.78-2.88(m, 4H), 2.35-2.5 l(m, 3H), 2.36(s, 3H), 1.85-2.01(m, 3H), 1.53-1.74(m , 2H),;
- the 4-nitrobenzyl bromide was replaced with 2,3,5-trifluoromethylbenzyl bromide, and the remaining starting materials, reagents and preparation methods were the same as those in Example 1, to obtain the target product DC35 in a yield of 80%.
- Example 37 2- ⁇ 3-Fluoro-[8-(2-fluoro-6-nitrobenzyl)] 8-azabicyclo[3.2.1]octane-3-methyl ⁇ -5,6-di
- DC48 methoxy-2,3-dihydro-1-indanone
- the 4-nitrobenzyl bromide was replaced by 2-fluoro-6-nitrobenzyl bromide, and the remaining starting materials, reagents and preparation methods were the same as those in Example 32 to obtain the target product DC48 in a yield of 80%.
- Example 50 2- ⁇ 3-Fluoro-[8-(biphenyl-4-methyl)]8-azabicyclo[3.2.1]octane-3-methyl ⁇ -5,6-dimethoxy
- DC76 2- ⁇ 3-Fluoro-[8-(biphenyl-4-methyl)]8-azabicyclo[3.2.1]octane-3-methyl ⁇ -5,6-dimethoxy
- the 4-nitrobenzyl bromide was replaced with biphenyl-4-methyl bromide, and the remaining starting materials, reagents and preparation methods were the same as those in Example 32 to obtain the desired product DC76 in a yield of 80%.
- Example 51 Preparation of 2-[(l-benzoyl-4-fluoropiperidinyl)methyl]-5,6-dimethoxy-2,3-dihydro-1-indanone (DC23)
- the 4-nitrobenzyl bromide was replaced with benzoyl chloride, and the remaining starting materials, reagents and preparation methods were the same as those in Example 1, to obtain the target product DC23.
- the target product is DCSOS ⁇ H NMR CCDCls, 400 MHz) ⁇ 7.48-7.56 (m, 3H), 7.04-7.21 (m, 3H), 5.56 (m, 2H), 4.46 ( m, 4H), 3.66 (s, 2H), 3.37 (m, lH), 2.58-2.83 (m, 2H), 1.62-2.24 (m, 4H), 1.56-1.62 (m, 6H); LRMS (EI) m/z 515(M + ).
- Example 83 Preparation of 2- ⁇ 4-fluoro-[1-(2-fluorobenzyl)]piperidin-4-methyl ⁇ -5,6-dichloro-2,3-dihydro-1-indanone (DC106) Replace 4-nitrobenzyl bromide with 2-fluorobenzyl bromide, 5,6-dimethoxyfluorenone with 5,6-dichlorofluorenone, and the remaining required raw materials, reagents and preparation methods are the same.
- Example 1 the target product DC 106 was obtained.
- Sample treatment All samples were formulated in 100% DMSO 10- 2 mol / L solution. Micromethod using 10- 2 mol / L solution was taken ⁇ ⁇ ⁇ do single tube, the reaction at a final concentration of 4x 10- 5 mo 1 / L.
- Solvent control The micro-method was measured with 0.4% DMSO, and the inhibition rate of the sample was subtracted from the solvent.
- Acetylcholinesterase zymogen rat cortical zymogen.
- Butyrylcholinesterase rat serum
- the percentage inhibition rate of most of the newly synthesized compounds is better than that of the positive control compound E2020, and the inhibition rate is more than 90%, which is nearly 30, which is much higher than E2020;
- the IC 5Q value of the compound for acetylcholinesterase is less than 1 nM, which is significantly better than the positive control drug E2020 (IC 5Q is 2 nM) o and the physicochemical properties (LogP, CLogP and tPSA, etc.) of the compound are comparable to the positive drug. , also has good drug properties.
- Sample treatment Undissolved at the time of sample delivery, add 5 % DMSO to fully oscillate and dissolve it, add 1% cosolvent EL (polyoxyethylene castor oil), and make up the remaining volume with water to make 10 mg/mL. The sample is suspended. liquid.
- Experimental animals KM mice, 22-29 g, male and female
- mice were randomized.
- the test compound DC 19 and B DC20 100 mg/kg were orally administered (the solvent control group was given an equal amount of 5% DMSO and 1% EL solution), and the mice were observed for obvious adverse reactions or death after administration.
- Table 2 Acute toxicity of compounds to mice
- Sample treatment Undissolved at the time of sample delivery, add 5 % DMSO to fully oscillate and dissolve it, add 1% cosolvent EL (polyoxyethylene castor oil), make up the remaining volume with water, mix 10 mg / mL, and gradually dilute to 0.3 mg/mL, 1 mg/mL, 3 mg/mL, and the sample was a suspension. Oral administration was carried out at a volume of 0.1 to mL/10 g by volume, and the solvent control group was orally administered an equal amount of 5% DMSO and 1% EL solution.
- cosolvent EL polyoxyethylene castor oil
- Acetylcholinesterase zymogen mouse cortex and hippocampus zymogen. After oral administration for 1 h in mice, the brain was removed after decapitation and the hippocampus and cortex were quickly stripped on ice. The cortex was homogenized in ice-cold 75 mM PBS to make a 30-fold tissue homogenate. The hippocampus was added to ice-cold 75 mM PBS homogenate. A 40-fold tissue homogenate was prepared and finally 1/10 volume of OMPA was added and placed on ice for testing.
- Test method The sample is added to the reaction system containing (PBS, H 2 0, S-ACh, DTNB), and the appropriate amount of enzyme is added to each tube except the blank tube. After reacting at room temperature for 20 minutes, the tubes are terminated by SDS. , blank tube plus appropriate amount of enzyme. The absorbance of each tube (OD440nm:) was measured by an ultraviolet-visible spectrophotometer.
- Solvent control 1 4 100.00 ⁇ 4.73 100.00 ⁇ 4.72 donepezil 10 4 73.50 ⁇ 7.50 * 64.91 ⁇ 6.82**
- DC20 significantly inhibited acetylcholinesterase in mouse cortex and hippocampus at doses of 1 mg/kg, 3 mg/kg, and 10 mg/kg.
- DC20 inhibited AChE in mouse cortex and hippocampus comparable to Donepezil, a 10 mg/kg positive control drug. Therefore, the overall activity of compound DC20 is significantly better than that of Donepezil.
- the inhibition of the mouse cortex and hippocampus acetylcholinesterase was determined by the compound of the formula I (R-configuration and S-configuration) and donepezil (positive control).
- Sample treatment Add 5% DMSO to fully oscillate and dissolve it, add 1% cosolvent EL (polyoxyethylene castor oil), make up the remaining volume with water, mix 10 mg/ml, and gradually dilute to 1 mg/ml, 0.1 Mg/ ml. Oral administration was carried out in a volume of 0.1 ml/10 g of the body weight, and the solvent control group was orally administered an equal amount of 5% DMSO and 1% EL solution.
- cosolvent EL polyoxyethylene castor oil
- Acetylcholinesterase zymogen mouse cortex and hippocampus zymogen.
- the cortex was homogenized in ice-cold 75 mM PBS to make a 30-fold tissue homogenate.
- the hippocampus was added to ice-cold 75 mM PBS.
- the slurry was made into a 40-fold tissue homogenate, and finally 1/10 volume of OMPA was added and placed on ice for testing.
- Test method Add the sample to the reaction system containing (PBS, H20, S-ACh, DTNB), add the appropriate amount of enzyme in addition to the blank tube, react at room temperature for 20 minutes, then terminate the reaction by adding SDS to each tube. Add appropriate amount of enzyme to the tube. The absorbance of each tube (OD440nm) was measured by an ultraviolet-visible spectrophotometer.
- the activity of acetylcholinesterase in the DCs of compound DC20-R 10 mg/kg and 1 mg/kg was 38.36% and 66.30%, respectively, and was inhibited by 61.64% and 33.70%, respectively, compared with the solvent control group;
- Compound DC20-S 10 mg/ The activity of acetylcholinesterase in hippocampus of kg 1 mg/kg was 36.66% and 63.84%, respectively, and was inhibited by 63.34% and 36.16%, respectively, compared with the solvent control group.
- DC20-R 10 mg/kg and 1 mg/kg have significant inhibitory effects on mouse cortex and hippocampal acetylcholinesterase.
- Compound DC20-S 10 mg/kg, 1 mg/kg cortex acetylcholinesterase activity was 68.94%, 94.51%, respectively, compared with the solvent control group inhibition of 31.06%, 5.49%; compound DC20-S 10 mg /
- the activity of acetylcholinesterase in hippocampus of kg 1 mg/kg was 65.94% and 86.19%, respectively, which was inhibited by 34.06% and 13.81%, respectively, compared with the solvent control group.
- DC20-S 10 mg/kg significantly inhibited mouse cortex and hippocampal acetylcholinesterase.
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Abstract
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JP2015538269A JP5990336B2 (ja) | 2012-10-26 | 2013-10-17 | フッ素置換環状アミン系化合物並びにその製造方法、薬物組成物および使用 |
BR112015009243-8A BR112015009243B1 (pt) | 2012-10-26 | 2013-10-17 | Composto de amina cíclico fluorosubstituído, método para preparar um composto de amina cíclico fluorosubstituído, composição farmacêutica, inibidor de acetilcolinesterase e uso do composto de amina cíclico fluorosubstituído |
KR1020157008760A KR101693128B1 (ko) | 2012-10-26 | 2013-10-17 | 플루오린 치환된 고리형 아민계 화합물 및 이의제조방법, 약물 조성물 및 용도 |
EP13849440.6A EP2913324B1 (en) | 2012-10-26 | 2013-10-17 | Fluorine substituted cyclic amine compounds and preparation methods, pharmaceutical compositions, and uses thereof |
RU2015114721/04A RU2605931C2 (ru) | 2012-10-26 | 2013-10-17 | Фторзамещенные циклические аминосоединения и способы их получения, фармацевтические композиции и их применения |
CA2888024A CA2888024C (en) | 2012-10-26 | 2013-10-17 | Fluorine substituted cyclic amine compounds and preparation methods, pharmaceutical compositions, and uses thereof |
US14/438,457 US9637454B2 (en) | 2012-10-26 | 2013-10-17 | Fluorine substituted cyclic amine compounds and preparation methods, pharmaceutical compositions, and uses thereof |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0296560A2 (en) | 1987-06-22 | 1988-12-28 | Eisai Co., Ltd. | 1,4-Substituted piperidines as acetylcholinesterase inhibitors and their use for the treatment of Alzheimer's disease |
WO2007102392A1 (ja) * | 2006-03-03 | 2007-09-13 | Shionogi & Co., Ltd. | Mmp-13選択的阻害剤 |
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JP4242032B2 (ja) | 1999-03-03 | 2009-03-18 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 4−置換ピペリジン誘導体フッ化物 |
WO2000051985A1 (fr) * | 1999-03-03 | 2000-09-08 | Eisai Co., Ltd. | Fluorures de derives de piperidine substitues en position 4 |
US7091218B1 (en) * | 1999-09-01 | 2006-08-15 | Eisai Co., Ltd. | 4-substituted piperidine compound |
JP4242048B2 (ja) | 1999-09-01 | 2009-03-18 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | 4−置換ピペリジン誘導体 |
WO2003061658A1 (fr) * | 2002-01-22 | 2003-07-31 | Eisai Co., Ltd. | Agent liant de récepteur sigma contenant un dérivé d'indanone |
BRPI0720211A2 (pt) | 2006-12-11 | 2013-12-24 | Reviva Pharmaceuticals Inc | Derivado de indanona, composição, e, uso de uma composição |
US9249098B2 (en) * | 2012-07-10 | 2016-02-02 | Xueheng Cheng | Derivatives of donepezil |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0296560A2 (en) | 1987-06-22 | 1988-12-28 | Eisai Co., Ltd. | 1,4-Substituted piperidines as acetylcholinesterase inhibitors and their use for the treatment of Alzheimer's disease |
WO2007102392A1 (ja) * | 2006-03-03 | 2007-09-13 | Shionogi & Co., Ltd. | Mmp-13選択的阻害剤 |
Non-Patent Citations (4)
Title |
---|
ACTA PHARMACOLOGICA SINICA, vol. 20, 1999, pages 141 - 5 |
BIOCHEM. PHARMACOL., vol. 7, 1961, pages 88 - 95 |
See also references of EP2913324A4 |
WORLD ALZHEIMER REPORT 2011, 2012 |
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WO2021104257A1 (zh) | 2019-11-26 | 2021-06-03 | 中国科学院上海药物研究所 | 一种乙酰胆碱酯酶抑制剂的晶型及其制备方法和应用 |
JP2023503511A (ja) * | 2019-11-26 | 2023-01-30 | 上海 インスティテュート オブ マテリア メディカ、チャイニーズ アカデミー オブ サイエンシーズ | アセチルコリンエステラーゼ阻害剤の結晶形およびその調製方法と適用 |
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JP5990336B2 (ja) | 2016-09-14 |
EP2913324A1 (en) | 2015-09-02 |
HK1210158A1 (zh) | 2016-05-27 |
KR20150063068A (ko) | 2015-06-08 |
CN103787954A (zh) | 2014-05-14 |
US9637454B2 (en) | 2017-05-02 |
RU2015114721A (ru) | 2016-11-10 |
BR112015009243A2 (pt) | 2019-12-10 |
EP2913324B1 (en) | 2017-12-20 |
US20150284331A1 (en) | 2015-10-08 |
KR101693128B1 (ko) | 2017-01-04 |
CA2888024A1 (en) | 2014-05-01 |
CA2888024C (en) | 2018-08-21 |
JP2015533839A (ja) | 2015-11-26 |
CN103787954B (zh) | 2016-01-20 |
RU2605931C2 (ru) | 2016-12-27 |
BR112015009243B1 (pt) | 2022-11-16 |
EP2913324A4 (en) | 2016-03-30 |
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