WO2013154304A3 - 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법 - Google Patents

핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법 Download PDF

Info

Publication number
WO2013154304A3
WO2013154304A3 PCT/KR2013/002895 KR2013002895W WO2013154304A3 WO 2013154304 A3 WO2013154304 A3 WO 2013154304A3 KR 2013002895 W KR2013002895 W KR 2013002895W WO 2013154304 A3 WO2013154304 A3 WO 2013154304A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detecting
preparing
high precision
polymerase
Prior art date
Application number
PCT/KR2013/002895
Other languages
English (en)
French (fr)
Other versions
WO2013154304A2 (ko
Inventor
박한오
이준희
김현서
최소라
김종훈
Original Assignee
(주)바이오니아
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)바이오니아 filed Critical (주)바이오니아
Priority to CN201380029805.2A priority Critical patent/CN104350159B/zh
Priority to US14/391,155 priority patent/US20150072351A1/en
Priority to EP13776121.9A priority patent/EP2837696B1/en
Priority to CN202111172838.5A priority patent/CN114410738A/zh
Publication of WO2013154304A2 publication Critical patent/WO2013154304A2/ko
Publication of WO2013154304A3 publication Critical patent/WO2013154304A3/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

본 발명은 고민감도 핵산준비방법에 관한 것으로， 상기 본 발명에 따른 핵산준비방법은 극미량의 핵산을 검출하기 위한 PCR 반응이나 실시간 정량 PCR 등 핵산중합반응에 앞서서 분석에 사용될 핵산들에 미리 핵산중합효소를 사용하여 터미네이터를 부가함으로써 타깃 핵산의 증폭반응과 경쟁적으로 일어나는 비특이적 프라이밍을 근본적으로 제거할 수 있어서 극미량의 타깃 핵산만을 정확하게 검출하고 타깃 핵산의 농도를 정확하게 측정할 수 있는 장점이 있다,
PCT/KR2013/002895 2012-04-09 2013-04-08 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법 WO2013154304A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN201380029805.2A CN104350159B (zh) 2012-04-09 2013-04-08 制备在利用核酸聚合酶来检测核酸时使用的高精度核酸的方法
US14/391,155 US20150072351A1 (en) 2012-04-09 2013-04-08 High-sensitivity nucleic acid preparation methods for the detection of nucleic acid by nucleic acid polymerase
EP13776121.9A EP2837696B1 (en) 2012-04-09 2013-04-08 High-sensitivity nucleic acid preparation methods for the detection of nucleic acid by nucleic acid polymerase
CN202111172838.5A CN114410738A (zh) 2012-04-09 2013-04-08 制备在利用核酸聚合酶来检测核酸时使用的高精度核酸的方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20120036978 2012-04-09
KR10-2012-0036978 2012-04-09
KR10-2012-0093222 2012-08-24
KR1020120093222A KR101545848B1 (ko) 2012-04-09 2012-08-24 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법

Publications (2)

Publication Number Publication Date
WO2013154304A2 WO2013154304A2 (ko) 2013-10-17
WO2013154304A3 true WO2013154304A3 (ko) 2013-12-19

Family

ID=49634569

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2013/002895 WO2013154304A2 (ko) 2012-04-09 2013-04-08 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법

Country Status (5)

Country Link
US (1) US20150072351A1 (ko)
EP (1) EP2837696B1 (ko)
KR (1) KR101545848B1 (ko)
CN (2) CN114410738A (ko)
WO (1) WO2013154304A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102013112602B4 (de) 2012-12-18 2020-11-12 Lg Display Co., Ltd. Weiße organische Lichtemissionsvorrichtung
EP3642361A1 (en) * 2017-06-20 2020-04-29 Ulisse Biomed S.P.A. Molecular fingerprinting methods to detect and genotype dna targets through polymerase chain reaction
CN109735607A (zh) * 2017-10-31 2019-05-10 中国科学院上海巴斯德研究所 一种双脱氧核苷封闭的寡核苷酸的制备方法及其应用
SG11201903333SA (en) 2017-12-29 2019-08-27 Clear Labs Inc Automated priming and library loading services
JP7090884B2 (ja) * 2018-04-02 2022-06-27 株式会社トクヤマデンタル 高誘電率を有する官能基を有する酸性基含有重合性単量体
US20220154180A1 (en) * 2019-03-13 2022-05-19 Toyobo Co., Ltd. Production and amplification of nucleic acids
WO2024006999A2 (en) * 2022-06-30 2024-01-04 Alnylam Pharmaceuticals, Inc. Cyclic-disulfide modified phosphate based oligonucleotide prodrugs

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US20070264655A1 (en) * 2006-05-09 2007-11-15 Canon Kabushiki Kaisha Nucleic acid automatic examining device

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5336760A (en) 1989-09-14 1994-08-09 Baxter International Inc. Method and useful apparatus for preparing pharmaceutical compositions
US5897783A (en) 1992-09-24 1999-04-27 Amersham International Plc Magnetic separation method
FI932866A0 (fi) 1993-06-21 1993-06-21 Labsystems Oy Separeringsfoerfarande
BE1007049A3 (nl) 1993-05-05 1995-02-28 Dsm Nv Werkwijze voor het verwijderen van kwik.
JP3115501B2 (ja) 1994-06-15 2000-12-11 プレシジョン・システム・サイエンス株式会社 分注機を利用した磁性体の脱着制御方法及びこの方法によって処理される各種装置
DE4423878A1 (de) 1994-07-07 1996-01-11 Boehringer Mannheim Gmbh Vorrichtung und Verfahren zum Abscheiden von magnetischen Mikropartikeln
CA2241170A1 (en) 1995-12-27 1997-07-10 Takara Shuzo Co., Ltd. Novel dna polymerase
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
JP2001519538A (ja) * 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US20020187477A1 (en) * 2001-06-06 2002-12-12 Hong Xue Method for detecting single nucleotide polymorphisms (SNPs) and point mutations
CA2497570A1 (en) * 2002-08-02 2004-02-12 Orchid Biosciences, Inc. Methods and compositions for genotyping
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
US8139210B2 (en) 2003-04-03 2012-03-20 Bioneer Corporation Real-time monitoring apparatus for biochemical reaction
AU2004277589A1 (en) * 2003-09-30 2005-04-14 Perkinelmer Las, Inc. Compositions and processes for genotyping single nucleotide polymorphisms
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7550264B2 (en) * 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2008005673A2 (en) * 2006-06-30 2008-01-10 Applera Corporation Reversible terminator nucleotides and methods of use
KR101350056B1 (ko) 2007-01-05 2014-01-15 엘지전자 주식회사 냉장고
KR20090025743A (ko) 2007-09-07 2009-03-11 주식회사 심텍 마이크로범프 온 패드 구조의 회로패턴 내장형인쇄회로기판 제조방법
EP2206135A1 (en) 2007-10-05 2010-07-14 E. I. du Pont de Nemours and Company Under-gate field emission triode with charge dissipation layer
KR101098764B1 (ko) 2007-10-29 2011-12-26 (주)바이오니아 안정화된 핫스타트 ｐｃｒ용 건조 조성물
US7846666B2 (en) * 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DE102008047790A1 (de) 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
US8632975B2 (en) * 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
KR101125475B1 (ko) 2010-08-06 2012-03-09 고려대학교 산학협력단 저항 변화층을 포함하는 단일 트랜지스터 플로팅 바디 디램 소자 및 그 제조 방법
WO2012083105A1 (en) * 2010-12-17 2012-06-21 Cocrystal Discovery, Inc. Inhibitors of hepatitis c virus polymerase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US20070264655A1 (en) * 2006-05-09 2007-11-15 Canon Kabushiki Kaisha Nucleic acid automatic examining device

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FINN, P. J. ET AL.: "Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing", NUCLEIC ACIDS RES., vol. 30, no. 13, 1 July 2002 (2002-07-01), pages 2877 - 2885, XP002243107 *
HUANG, P. ET AL.: "Selective action of 3'-azido-3'-deoxythymidine 5'-triphosphate on viral reverse transcriptases and human DNA polymerases", J. BIOL. CHEM., vol. 265, no. 20, 15 July 1990 (1990-07-15), pages 11914 - 11918, XP055166476 *
SMITH, A. J. H. ET AL.: "The use of exonuclease III for preparing single stranded DNA for use as a template in the chain terminator sequencing method", NUCLEIC ACIDS RES., vol. 6, no. 3, March 1979 (1979-03-01), pages 831 - 848, XP055166475 *

Also Published As

Publication number Publication date
EP2837696B1 (en) 2018-01-31
CN104350159B (zh) 2021-10-22
WO2013154304A2 (ko) 2013-10-17
EP2837696A4 (en) 2016-03-16
KR20130114553A (ko) 2013-10-17
CN114410738A (zh) 2022-04-29
CN104350159A (zh) 2015-02-11
EP2837696A2 (en) 2015-02-18
US20150072351A1 (en) 2015-03-12
KR101545848B1 (ko) 2015-08-21

Similar Documents

Publication Publication Date Title
WO2013154304A3 (ko) 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
WO2013130512A3 (en) Methods and uses for molecular tags
WO2016130868A3 (en) Materials and methods to analyze rna isoforms in transcriptomes
WO2016064894A3 (en) Compositions and methods for detecting an rna virus
WO2012095639A3 (en) Methods, compositions, and kits for determing the presence/absence of a varian nucleic acid sequence
MX338296B (es) Composiciones y metodos para cuantificar una secuencia de acido nucleico en una muestra.
IL233773B (en) Methods for identifying a target sequence of a nucleic acid from DNA or a mixture of nucleic acids and a kit for performing these methods
EP3967769A3 (en) Lung cancer detection kit or device, and detection method
EP3816302A3 (en) Esophageal cancer detection kit or device, and detection method
WO2013184754A3 (en) Spatial sequencing of nucleic acids using dna origami probes
EP3971299A3 (en) Colorectal cancer detection kit or device, and detection method
MX2016016898A (es) Metodos y composiciones para analisis de muestras.
WO2012145725A3 (en) Oscillating amplification reaction for nucleic acids
WO2012135815A3 (en) Methods and kits for detecting cell-free pathogen-specific nucleic acids
EP3757225A3 (en) Pancreatic cancer detection kit or device, and detection method
EP3862439A3 (en) Liver cancer detection kit or device, and detection method
WO2011143478A3 (en) Identification of nucleic acids
WO2013050881A3 (en) Direct nucleic acid analysis
WO2014005969A9 (fr) Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees
WO2011153354A8 (en) Methods and kits for in situ detection of nucleotide sequences
WO2014152281A3 (en) Ribosomal ribonucleic acid hybridization for organism identification
EP3512965A4 (en) METHOD FOR PRODUCING NUCLEIC ACIDS FOR ANALYZING CELL-FREE DNA
EP3347497A4 (en) NUCLEIC ACID ANALYSIS BY ASSEMBLING POLYNUCLEOTIDE BARCODE PROBES
IN2014CN03508A (ko)
MX2015001961A (es) Metodos y sistemas para ensayos.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13776121

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 14391155

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2013776121

Country of ref document: EP