WO2013137027A1 - 分離膜モジュールの滅菌方法、連続発酵による化学品の製造方法、および膜分離型連続発酵装置 - Google Patents
分離膜モジュールの滅菌方法、連続発酵による化学品の製造方法、および膜分離型連続発酵装置 Download PDFInfo
- Publication number
- WO2013137027A1 WO2013137027A1 PCT/JP2013/055711 JP2013055711W WO2013137027A1 WO 2013137027 A1 WO2013137027 A1 WO 2013137027A1 JP 2013055711 W JP2013055711 W JP 2013055711W WO 2013137027 A1 WO2013137027 A1 WO 2013137027A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- separation membrane
- membrane module
- liquid
- secondary side
- water
- Prior art date
Links
- 239000012528 membrane Substances 0.000 title claims abstract description 711
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 248
- 238000000855 fermentation Methods 0.000 title claims abstract description 172
- 230000004151 fermentation Effects 0.000 title claims abstract description 172
- 238000000034 method Methods 0.000 title claims abstract description 83
- 239000000126 substance Substances 0.000 title claims abstract description 58
- 238000004519 manufacturing process Methods 0.000 title abstract description 27
- 238000000926 separation method Methods 0.000 claims abstract description 599
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 340
- 239000007788 liquid Substances 0.000 claims abstract description 277
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 216
- 238000011049 filling Methods 0.000 claims abstract description 18
- 238000009835 boiling Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims description 152
- 238000004140 cleaning Methods 0.000 claims description 68
- 244000005700 microbiome Species 0.000 claims description 46
- 230000008569 process Effects 0.000 claims description 33
- 239000002994 raw material Substances 0.000 claims description 32
- 239000000047 product Substances 0.000 claims description 28
- 238000001816 cooling Methods 0.000 claims description 21
- 238000007789 sealing Methods 0.000 claims description 19
- 239000000706 filtrate Substances 0.000 claims description 15
- 238000007599 discharging Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 2
- 239000012510 hollow fiber Substances 0.000 description 79
- 239000000243 solution Substances 0.000 description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 42
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 42
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 32
- 238000011282 treatment Methods 0.000 description 30
- 239000007864 aqueous solution Substances 0.000 description 28
- 210000004748 cultured cell Anatomy 0.000 description 28
- 239000011148 porous material Substances 0.000 description 27
- 239000007789 gas Substances 0.000 description 22
- 235000019441 ethanol Nutrition 0.000 description 21
- 230000004907 flux Effects 0.000 description 21
- 235000011187 glycerol Nutrition 0.000 description 20
- 229920005989 resin Polymers 0.000 description 18
- 239000011347 resin Substances 0.000 description 18
- 239000004310 lactic acid Substances 0.000 description 16
- 235000014655 lactic acid Nutrition 0.000 description 16
- 239000003795 chemical substances by application Substances 0.000 description 15
- 239000002609 medium Substances 0.000 description 15
- 238000004382 potting Methods 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 230000002441 reversible effect Effects 0.000 description 12
- 229920006395 saturated elastomer Polymers 0.000 description 12
- 230000002209 hydrophobic effect Effects 0.000 description 11
- 238000011085 pressure filtration Methods 0.000 description 11
- 238000010586 diagram Methods 0.000 description 10
- 238000001035 drying Methods 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 9
- 238000012986 modification Methods 0.000 description 9
- 230000004048 modification Effects 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 239000000306 component Substances 0.000 description 7
- 238000011109 contamination Methods 0.000 description 7
- BQCIDUSAKPWEOX-UHFFFAOYSA-N 1,1-Difluoroethene Chemical compound FC(F)=C BQCIDUSAKPWEOX-UHFFFAOYSA-N 0.000 description 6
- YEJRWHAVMIAJKC-UHFFFAOYSA-N 4-Butyrolactone Chemical compound O=C1CCCO1 YEJRWHAVMIAJKC-UHFFFAOYSA-N 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 238000001223 reverse osmosis Methods 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 5
- 229930182843 D-Lactic acid Natural products 0.000 description 5
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 150000001298 alcohols Chemical class 0.000 description 5
- 239000003638 chemical reducing agent Substances 0.000 description 5
- 229940022769 d- lactic acid Drugs 0.000 description 5
- 230000006866 deterioration Effects 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 150000007524 organic acids Chemical class 0.000 description 5
- 239000012466 permeate Substances 0.000 description 5
- 239000012071 phase Substances 0.000 description 5
- 239000011550 stock solution Substances 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 239000002033 PVDF binder Substances 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000005538 encapsulation Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000003472 neutralizing effect Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 235000005985 organic acids Nutrition 0.000 description 4
- 239000007800 oxidant agent Substances 0.000 description 4
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 description 3
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229920001519 homopolymer Polymers 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 235000013379 molasses Nutrition 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 229920002492 poly(sulfone) Polymers 0.000 description 3
- -1 polypropylene Polymers 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- BBMCTIGTTCKYKF-UHFFFAOYSA-N 1-heptanol Chemical compound CCCCCCCO BBMCTIGTTCKYKF-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- SECXISVLQFMRJM-UHFFFAOYSA-N N-Methylpyrrolidone Chemical compound CN1CCCC1=O SECXISVLQFMRJM-UHFFFAOYSA-N 0.000 description 2
- 239000004695 Polyether sulfone Substances 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 238000007664 blowing Methods 0.000 description 2
- WERYXYBDKMZEQL-UHFFFAOYSA-N butane-1,4-diol Chemical compound OCCCCO WERYXYBDKMZEQL-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229920000620 organic polymer Polymers 0.000 description 2
- 238000010979 pH adjustment Methods 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 229920006350 polyacrylonitrile resin Polymers 0.000 description 2
- 229920006393 polyether sulfone Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 229920002803 thermoplastic polyurethane Polymers 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 description 1
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 1
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- YPSIXSGKHOISQD-UHFFFAOYSA-N C=C.F.Cl.Cl.Cl Chemical compound C=C.F.Cl.Cl.Cl YPSIXSGKHOISQD-UHFFFAOYSA-N 0.000 description 1
- 229920008347 Cellulose acetate propionate Polymers 0.000 description 1
- 229920002284 Cellulose triacetate Polymers 0.000 description 1
- 241000186031 Corynebacteriaceae Species 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 235000019766 L-Lysine Nutrition 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 238000011001 backwashing Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000012461 cellulose resin Substances 0.000 description 1
- 238000012824 chemical production Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 238000004388 gamma ray sterilization Methods 0.000 description 1
- 229960002989 glutamic acid Drugs 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- HCDGVLDPFQMKDK-UHFFFAOYSA-N hexafluoropropylene Chemical group FC(F)=C(F)C(F)(F)F HCDGVLDPFQMKDK-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000020121 low-fat milk Nutrition 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229920013716 polyethylene resin Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920000166 polytrimethylene carbonate Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000020083 shōchū Nutrition 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- ZIBGPFATKBEMQZ-UHFFFAOYSA-N triethylene glycol Chemical compound OCCOCCOCCO ZIBGPFATKBEMQZ-UHFFFAOYSA-N 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
- A61L2/07—Steam
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
- B01D65/022—Membrane sterilisation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0081—After-treatment of organic or inorganic membranes
- B01D67/0093—Chemical modification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/08—Hollow fibre membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/12—Composite membranes; Ultra-thin membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/06—Organic material
- B01D71/30—Polyalkenyl halides
- B01D71/32—Polyalkenyl halides containing fluorine atoms
- B01D71/34—Polyvinylidene fluoride
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/17—Combination with washing or cleaning means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2321/00—Details relating to membrane cleaning, regeneration, sterilization or to the prevention of fouling
- B01D2321/08—Use of hot water or water vapor
Definitions
- the present invention relates to a method for sterilizing a separation membrane module used when filtering microorganisms from a fermentation broth to obtain a chemical contained in the fermentation broth, a method for producing a chemical by continuous fermentation, and a membrane separation type continuous
- the present invention relates to a fermentation apparatus.
- Fermentation methods which are substance production methods involving the cultivation of microorganisms and cultured cells, are largely divided into (1) batch fermentation methods (Batch fermentation methods) and fed-batch fermentation methods (Fed-Batch fermentation methods), and (2) continuous fermentation methods. Can be classified.
- the batch fermentation method and fed-batch fermentation method of (1) above are simple in terms of equipment, and have the advantage that culture is completed in a short time and damage caused by various bacteria is small.
- the chemical concentration in the fermentation broth increases with the passage of time, and productivity and yield decrease due to osmotic pressure or chemical inhibition. Therefore, it is difficult to stably maintain a high yield and high productivity over a long period of time.
- the continuous fermentation method (2) is characterized in that high yield and high productivity can be maintained over a long period of time by avoiding accumulation of the target chemical product at a high concentration in the fermenter.
- a continuous culture method for fermentation of L-glutamic acid or L-lysine is disclosed (see Non-Patent Document 1).
- the raw material is continuously supplied to the fermentation broth and the fermentation broth containing microorganisms and cultured cells is extracted, the microorganisms and cultured cells in the fermentation broth are diluted. The improvement in production efficiency was limited.
- microorganisms and cultured cells are filtered through a separation membrane, and chemicals are collected from the filtrate. At the same time, the microorganisms and cultured cells in the concentrated liquid are retained or refluxed in the fermentation broth.
- a method for maintaining a high concentration of microorganisms and cultured cells has been proposed. For example, in a continuous fermentation apparatus using a flat membrane made of an organic polymer as a separation membrane, a technique for continuous fermentation has been proposed (see Patent Document 1).
- sterilization methods include flame sterilization, dry heat sterilization, boiling sterilization, steam sterilization, ultraviolet sterilization, gamma ray sterilization, and gas sterilization. Note that the separation function is lost. Although there is a method of sterilizing with a drug, there is a problem of processing of the drug after sterilization or the drug remaining in the separation membrane module. Furthermore, there is concern that drug-resistant microorganisms remain.
- the shape of the separation membrane includes a flat membrane, a hollow fiber membrane, a spiral type, and the hollow membrane module includes an external pressure type and an internal pressure type.
- the hollow fiber membrane module has a large membrane area per unit device and is considered to be an industrially useful structure, but the structure is complicated.
- steam at a predetermined temperature and pressure for example, saturated steam at 125 ° C. is supplied to the fermenter and peripheral equipment, and general steam sterilization is performed.
- a predetermined temperature and pressure for example, saturated steam at 125 ° C.
- Each facility is heated up to 121 ° C., which is the temperature of this, and the sterilization temperature is maintained for a predetermined time (20 minutes or more) to perform steam sterilization.
- Patent Document 2 As a method of steam sterilization, steam sterilization may be performed by steaming the outside of the hollow fiber membrane (primary side) during steam sterilization, or by further steaming the inside of the hollow fiber membrane (secondary side). It has been proposed (Patent Document 2).
- Patent Document 2 As a simulation test at the time of long-term operation of the hollow fiber membrane module by the steam sterilization method, water is injected into the hollow fiber membrane module and water vapor is repeatedly injected to evaluate leakage. Water is not sealed in the secondary side of the membrane module and steam sterilized.
- the device is cooled as it is after the steam sterilization, the water vapor is condensed and the inside of the device becomes a negative pressure, which may cause contamination with germs.
- the supply temperature of water vapor is set to be equal to or higher than a predetermined steam sterilization temperature even in a place where the temperature is most difficult to rise (cold spot).
- the steam supply time is set so that the place where the temperature is most difficult to rise rises above a predetermined steam sterilization temperature and then sterilizes for a predetermined steam sterilization time or longer.
- heat dissipation measures are taken, such as heat retention, but the temperature of the supplied water vapor is set to 121 ° C. or higher.
- a urethane-based or epoxy-based potting agent is generally used to fix the hollow fiber membrane and the module container. This potting agent may deteriorate due to repeated steam sterilization, and the potting agent and the hollow fiber membrane or the potting agent and the module container may be peeled off.
- a urethane-based resin having a high elongation may be used as a potting agent.
- hydrophilic separation membrane If it is a hydrophilic separation membrane, it can be easily replaced because it gets wet with water, but many separation membranes based on hydrophobic substances have the required performance such as chemical resistance and heat resistance. In order to replace the gas phase in the pores of the separation membrane with the liquid phase, for example, it is necessary to replace with a liquid having an affinity for the hydrophobic membrane and then replace with water.
- the present invention has been made in view of the above, and a method for sterilizing a separation membrane module that can reliably sterilize a separation membrane module and suppress drying of the separation membrane in a short time, and production of a chemical by continuous fermentation A method and a membrane separation type continuous fermentation apparatus are provided.
- the separation membrane module sterilization method of the present invention is a method of sterilizing a separation membrane module with water vapor, and on the secondary side of the separation membrane module, A liquid supply step of supplying a liquid having a boiling point of 80 ° C. or higher under atmospheric pressure so that a filling rate in a space surrounded by a portion to be filtered is 70% or more, and the liquid supply step A liquid sealing step for sealing the secondary side so that the filling rate of the liquid supplied to the secondary side of the separation membrane module is 70% or more, and while the secondary side of the separation membrane module is sealed And sterilizing the separation membrane module by supplying water vapor to the primary side of the separation membrane module.
- the method for producing a chemical product by continuous fermentation includes a steam sterilization step for sterilizing the separation membrane module by the sterilization method, and a fermentation raw material is converted into a fermentation broth containing the chemical product by fermentation culture of microorganisms. It includes a fermentation step and a membrane separation step of collecting a chemical product as a filtrate from the fermentation broth by the separation membrane module after the steam sterilization step.
- the membrane separation type continuous fermentation apparatus of the present invention comprises a fermenter for converting a fermentation raw material into a fermentation broth containing a chemical by fermenting and culturing the fermentation raw material with a microorganism, and a chemical from the fermentation broth.
- a separation membrane module to be separated a fermentation liquid circulation section for feeding a fermentation liquid from the fermentation tank to the separation membrane module; a steam supply section for supplying water vapor to the fermentation tank and the separation membrane module; and the separation membrane module
- a liquid supply unit for supplying a liquid having a boiling point of 80 ° C.
- a sealing portion that seals a secondary side of the separation membrane module so that a filling rate of the liquid in a space surrounded by a portion to be filtered is 70% or more.
- the separation membrane module is heated to a predetermined sterilization temperature by enclosing a liquid at 80 ° C. or higher under atmospheric pressure on the secondary side of the separation membrane module and then supplying water vapor to the primary side. Since the time required to do so can be significantly shortened, it is possible to suppress deterioration of the potting agent due to heat, and it is possible to suppress drying of the separation membrane. Furthermore, since air is not used for cooling or the like, it is possible to suppress damage to the separation membrane and a decrease in the amount of permeated water.
- FIG. 1 is a schematic diagram of an apparatus for sterilizing a separation membrane module according to Embodiment 1 of the present invention.
- FIG. 2 is a flowchart illustrating the steam sterilization process according to the first embodiment of the present invention.
- FIG. 3 is a schematic diagram of an apparatus for sterilizing a separation membrane module according to Modification 1 of Embodiment 1 of the present invention.
- FIG. 4 is a schematic diagram of an apparatus for sterilizing a separation membrane module according to Modification 2 of Embodiment 1 of the present invention.
- FIG. 5 is a schematic diagram of a membrane separation type continuous fermentation apparatus according to Embodiment 2 of the present invention.
- FIG. 6 is a flowchart for explaining a sterilization process according to the second embodiment of the present invention.
- FIG. 7 is a schematic diagram of a membrane separation type continuous fermentation apparatus according to Modification 1 of Embodiment 2 of the present invention.
- FIG. 1 is a schematic diagram of an apparatus for sterilizing a separation membrane module according to Embodiment 1 of the present invention.
- the sterilization apparatus 100 includes a vapor supply unit 20 that supplies water vapor to the primary side of the separation membrane module 2, and a liquid that supplies a liquid having a boiling point of 80 ° C. or higher at atmospheric pressure to the secondary side of the separation membrane module 2. And a supply unit 40.
- the separation membrane module 2 is connected to a circulation valve 17 and a pipe 23 for supplying a raw solution to be processed to the primary side, and the filtrate that is filtered through the separation membrane is discharged to the outside of the separation membrane module 2
- a liquid discharge line 24 is connected to the secondary side.
- the filtrate discharge line 24 is provided with a filtration pump 11 and a filtration valve 13, and the filtration valve 13 is opened, and suction is performed by the filtration pump 11, so that the filtrate is filtered from the primary side to the secondary side.
- the stock solution that has not been filtered to the secondary side is cross-flowed through the pipe 25.
- the steam supply unit 20 is connected to the primary side of the separation membrane module 2 via the supply valve 19 and the pipe 34.
- the steam at a predetermined temperature supplied from the steam supply unit 20 to the primary side of the separation membrane module 2 is discharged out of the separation membrane module 2 via the discharge line 33 and the discharge valve 32.
- the liquid supplied from the liquid supply unit 40 to the primary side of the separation membrane module 2 is supplied to the secondary side through the separation membrane.
- the filtration of the liquid to the secondary side is preferably performed while suctioning with the filtration pump 11.
- the liquid supplied to the primary side of the separation membrane module 2 is discharged out of the system of the separation membrane module 2 through a pipe 25 for crossflow.
- the side in contact with the undiluted solution to be processed in the separation membrane module 2 is called a primary side
- the side in contact with the filtrate after processing is called a secondary side.
- the separation membrane module 2 includes a separation membrane and a container that accommodates the separation membrane.
- the separation membrane used in Embodiment 1 may be an organic membrane or an inorganic membrane. Since the separation membrane is washed by reverse pressure washing or chemical solution immersion, the separation membrane preferably has durability against these.
- As the shape of the separation membrane any shape such as a flat membrane, a hollow fiber membrane, and a spiral type can be adopted. Among these, a hollow fiber membrane module is preferable, and any one of an external pressure type and an internal pressure type can be adopted as long as it is a hollow fiber membrane module.
- an organic polymer compound can be suitably used from the viewpoints of separation performance, water permeability, and dirt resistance.
- examples include polyethylene resins, polypropylene resins, polyvinyl chloride resins, polyvinylidene fluoride resins, polysulfone resins, polyethersulfone resins, polyacrylonitrile resins, cellulose resins, and cellulose triacetate resins. A mixture of these resins as the main component may be used.
- a vinylidene chloride resin or a resin containing the vinylidene fluoride resin as a main component is more preferably used because it has a characteristic of having both chemical strength (particularly chemical resistance) and physical strength.
- the polyvinylidene fluoride-based resin a homopolymer of vinylidene fluoride is preferably used.
- the polyvinylidene fluoride resin may be a copolymer of a vinyl monomer copolymerizable with vinylidene fluoride.
- vinyl monomers copolymerizable with vinylidene fluoride include tetrafluoroethylene, hexafluoropropylene, and ethylene trichloride fluoride.
- the average pore diameter of the separation membrane used in Embodiment 1 can be appropriately determined according to the purpose and situation of use, but it is preferable that the average pore diameter is somewhat small, and is usually 0.01 ⁇ m or more and 1 ⁇ m or less. preferable.
- the average pore diameter of the hollow fiber membrane is less than 0.01 ⁇ m, components such as sugar and protein and membrane dirt components such as aggregates block the pores, and stable operation cannot be performed. In consideration of the balance with water permeability, it is preferably 0.02 ⁇ m or more, and more preferably 0.03 ⁇ m or more.
- the average pore size approaches the size of the microorganism or cultured cell, these may directly block the pores.
- the average pore diameter is 0.4 ⁇ m. The following is preferable, and 0.2 ⁇ m or less is preferable.
- the average pore diameter of the separation membrane can be obtained by measuring and averaging the diameters of a plurality of pores observed by scanning electron microscope observation at a magnification of 10,000 times or more.
- 10 or more, preferably 20 or more pores are randomly selected, the diameters of these pores are measured, and the number average is obtained.
- an image processing device or the like it is also preferable to use an image processing device or the like to obtain a circle having an area equal to the area of the pores, that is, an equivalent circle, and obtain the equivalent circle diameter as the pore diameter. it can.
- the separation membrane module 2 When performing the filtration treatment using the separation membrane module 2, it is preferable to perform the steam sterilization treatment of the separation membrane module 2 before the filtration treatment in order to prevent contamination of the apparatus and / or the filtrate with various bacteria.
- Embodiment 1 before supplying water vapor from the vapor supply unit 20 to the primary side of the separation membrane module 2, it is preferable that the liquid is sealed on the secondary side of the separation membrane module 2 by the liquid supply unit 40, Furthermore, it is preferable that water vapor is supplied from the vapor supply unit 20 while maintaining the state in which the liquid is sealed.
- a liquid having a high boiling point for example, a liquid having a boiling point of 80 ° C. or higher under atmospheric pressure is sealed on the secondary side of the separation membrane module 2, so that the sealed liquid is separated from the separation membrane module 2.
- the heat-up time to the predetermined sterilization temperature of the separation membrane module 2 than when no liquid is sealed by conducting heat from the water vapor supplied to the primary side to the respective parts of the separation membrane module 2 through the separation membrane Can be shortened. As a result, the thermal load on the separation membrane module 2 can be reduced.
- a liquid has a higher thermal conductivity than a gas (for example, the thermal conductivity of water is larger than the thermal conductivity of air and the thermal conductivity of water vapor), so a liquid is provided on the secondary side of the separation membrane module 2.
- the temperature rise rate of the separation membrane module 2 becomes faster than when air or water vapor exists on the secondary side.
- a smaller heat capacity of the liquid to be sealed is advantageous for raising the temperature, and the heat conduction inside the separation membrane module 2 is considered to be affected by the magnitude of the heat capacity.
- the water vapor may be able to pass through the separation membrane from the primary side to the secondary side. Therefore, if the liquid is previously sealed on the secondary side before heating with water vapor, when the pressurized water vapor is supplied to the primary side, a part of the pressurized water vapor on the primary side is 2 There is room for water vapor to enter the secondary side by passing to the secondary side, liquid passing through the separation membrane from the secondary side to the primary side, or the temperature of the secondary side liquid becoming high and evaporating. Can do. As a result, water vapor and liquid can be exchanged on the secondary side of the membrane.
- the film can be heated with water vapor from the secondary side.
- steam is ventilated without enclosing a liquid on the secondary side
- the water in the separation membrane hole comes into contact with saturated steam during steam sterilization, thereby achieving an equilibrium state.
- the moisture in the separation membrane pores gradually decreased and the separation membrane was dried.
- the water vapor does not always pass through the hollow fiber membrane uniformly, a part of the air existing on the secondary side of the hollow fiber membrane remains before the water vapor is passed, and the air is locked. There was a concern of staying in a state (that is, a state where an air lock was generated).
- the pressure applied to the primary side of the separation membrane during steam sterilization depends on the material of the membrane, but is often less than the bubble point particularly in a hydrophobic separation membrane. For example, in normal steam sterilization, the pressure condition is about 0.13 MPa because the saturated water vapor pressure is about 121 ° C.
- “enclosing” means sealing a space containing a liquid so that the liquid does not flow out of the space.
- sealing means separating a predetermined space from an external space.
- Separatate from outside space can be rephrased as “separate from outside space”.
- “Sealing” means, in particular, closing a path through which liquid flows out from the space on the secondary side of the separation membrane in the separation membrane module.
- Specific means for sealing include closing a valve on a path that is connected to the separation membrane module and the liquid flows out from the secondary side of the separation membrane, and closing the valve. Specifically, a state in which the valves 13 and 27 provided on the lines 24 and 26 connected to the separation membrane module 2 are closed so as not to allow liquid to pass is a “sealed” state. Valves 14 and 22 are also closed if necessary to seal. However, as will be described later, in the case of steam sterilization while performing reverse pressure filtration, the valve 22 is opened.
- the liquid may pass through the separation membrane, but such passage of the liquid through the separation membrane does not correspond to “outflow”. That is, even if the liquid passes through the separation membrane, it is included in the “sealed” state.
- Encapsulation and “sealing” do not mean excluding any outflow other than through the separation membrane. That is, as described above, if the effect of improving the sterilization efficiency by the enclosed liquid can be obtained, the outflow of the liquid is not excluded. A decrease in filling rate after the start of steam sterilization is acceptable. Moreover, reverse pressure filtration, that is, supplying liquid to the secondary side and allowing the liquid to pass from the secondary side to the primary side through the separation membrane is also included in the “encapsulation” and “sealing” of the present invention. Applicable. Details will be described later.
- the sterilization temperature of general steam sterilization is 121 ° C.
- the boiling point of the liquid to be sealed under atmospheric pressure is 80 ° C. or more in order to reduce the influence on the separation membrane module 2 due to vaporization of the liquid to be sealed. It is preferable that When sterilizing at a sterilization temperature lower than 121 ° C., a liquid having a boiling point of 80 ° C. or lower under atmospheric pressure can be selected as the sealed liquid.
- the liquid supplied to the secondary side of the separation membrane module 2 is preferably water such as ion-exchanged water, reverse osmosis membrane permeated water, distilled water, or alcohol.
- alcohols include monohydric alcohols such as 1-butanol, 2-butanol and 1-heptanol, ethylene glycol, 1,2-propanediol, 1,3-propanediol, diethylene glycol, triethylene glycol, glycerin and the like.
- butyl cellosolve, phenyl cellosolve and the like are exemplified. Silicone oil or water to which a surfactant is added can also be used.
- an electrolyte dissolved in water may be used, or an alkali, acid, oxidizing agent or reducing agent may be added.
- an alkali, acid, oxidizing agent or reducing agent may be added.
- water without additives is suitable as the sealed liquid.
- the liquid to be sealed has a higher affinity with the separation membrane, and it becomes easier to enclose the liquid on the secondary side of the separation membrane module 2. Therefore, when the separation membrane is hydrophilic, it is preferable to select a hydrophilic liquid, and to select a hydrophobic liquid for the hydrophobic separation membrane. Alternatively, even when a hydrophobic separation membrane is used, the hydrophobic separation membrane is immersed in glycerin that is compatible with the hydrophilic liquid used for encapsulation and has a high affinity with the hydrophobic separation membrane. By doing so, it can be selected as a hydrophilic liquid, for example, a liquid enclosing water.
- the temperature of the liquid to be sealed is not particularly specified because sterilization can be performed if the temperature can be raised to a predetermined temperature during steam sterilization.
- the sealed liquid sealed on the secondary side of the separation membrane is immediately heated by the supplied steam, the temperature rises. There is no big difference in time.
- the method for enclosing the liquid on the secondary side of the separation membrane is not particularly limited, but will be described as follows by taking the case where the liquid is water as an example.
- pressure is applied to the primary side of the separation membrane module 2, or Suction from the secondary side, filter from the primary side to the secondary side, fill the secondary side with water, close the discharge valve 27 and the filtration valve 13, and enclose the secondary side with water To do.
- water is supplied from the primary side to the secondary side of the separation membrane module 2 by filtration, if the filtration time is short, there is a concern that the secondary side of the separation membrane may not be sufficiently sealed with water.
- the primary side of the hollow fiber membrane is filled with water, and then filtered with a filtration flux of 0.2 m / d for 15 minutes or more. More than 90% of the secondary volume of the part can be filled with water.
- the filtration flux at the time of enclosing the secondary side is preferably larger because it can be encapsulated quickly and the working time is shortened, and air in the pores of the separation membrane is easily pushed out.
- the filtration flux when the liquid is sealed on the secondary side is preferably 0.1 m / d or more, and more preferably 0.2 m / d or more.
- the amount of water enclosed in the secondary side of the separation membrane module 2 is preferably 70% or more with respect to the secondary side volume of the filtration portion of the separation membrane. If it is less than 70%, there is a concern that the film is partially dried.
- the secondary volume is the secondary volume of the separation membrane with respect to the effective membrane area of the separation membrane.
- a hollow fiber membrane module is used as the separation membrane module 2, in order to fix the hollow fiber membrane in the separation membrane module 2, it is fixed with an adhesive serving as a potting agent, but the hollow fiber membrane in the potting layer is Since the surrounding is a potting agent, it does not contribute to filtration and does not enter the effective membrane area. Therefore, the secondary volume is not counted.
- the secondary side volume can be calculated from the inner diameter value of the hollow fiber membrane and the hollow fiber membrane length of the effective membrane area portion.
- An external pressure type hollow fiber membrane generally has a circular cross-sectional area, but a shape such as a triangle or a square can be easily calculated.
- water may be once sealed on the secondary side of the separation membrane, and the water may be discharged and measured.
- the volume of the effective membrane area can be calculated by subtracting the volume of the water discharged together.
- the amount of water enclosed on the secondary side of the separation membrane is determined by stopping the supply of water to the separation membrane module 2, filling the water by valve operation, and then discharging the primary side water of the separation membrane module 2 once. Then, it can be measured by discharging the water on the secondary side. For example, after filtering from the primary side to the secondary side of the separation membrane, a valve installed on the secondary side is closed and water is sealed on the secondary side. Then, after draining the water on the primary side of the separation membrane, the valve installed on the secondary side is opened, and the secondary side is pressurized with air as necessary. Drain and weigh. In this case, water that has filled the secondary liquid supply line, etc.
- the amount of water in the liquid supply line is measured in advance, the amount of water on the secondary side of the hollow fiber membrane should be obtained. Can do.
- the water encapsulation rate can be determined by observing from the primary side of the separation membrane and measuring the length of the water-enclosed portion and the portion where the gas remains. Although it is desirable that the entire separation membrane can be observed, since there are portions that cannot be visually observed, partial observation may be used.
- the mass of the separation membrane module 2 is weighed in advance, the water is sealed on the secondary side, and the water on the primary side is discharged.
- the amount of water enclosed on the secondary side can also be determined by measuring the mass. In this case as well, water that has filled the secondary liquid supply line is included, but if the amount of water in the liquid supply line is measured in advance, the amount of water on the secondary side of the hollow fiber membrane is obtained. be able to.
- water Before filling the entire primary side of the separation membrane with water, water can be supplied to the secondary side by applying pressure to the primary side or sucking the secondary side. It is preferable to filter the water from the primary side to the secondary side after filling the entire primary side with water because the water is more quickly sealed to the secondary side.
- the sealing method is not particularly limited.
- the primary pressure should not be lower than the secondary pressure. It is necessary to keep the amount of water on the secondary side to be 70% or more.
- the steam supply unit 20 supplies water vapor to the primary side of the separation membrane module 2. What is necessary is just to set the temperature of the water vapor
- the water vapor to be supplied it is preferable to use ion exchange water, reverse osmosis membrane treated water, distilled water, or water having the same level of cleanliness.
- Water for steam may be preliminarily sterilized with ion exchange water, reverse osmosis membrane treated water, distilled water, etc., and then may be used as predetermined steam, and ion exchange water, reverse osmosis membrane treated water, distilled water, etc. Steam at a temperature may be used, followed by sterilization through a sterilization filter or the like.
- Sterilization of the separation membrane module 2 is performed by raising the temperature of the separation membrane module to a predetermined temperature and maintaining that temperature for a predetermined time.
- sterilization is preferably performed by raising the temperature to 121 ° C. or higher and holding for 15 to 20 minutes.
- the sterilization process can include a temperature raising process for increasing the temperature and a temperature maintaining process for maintaining the temperature. Whether the temperature of the separation membrane module is increased to an appropriate value during sterilization can be determined as follows.
- the separation membrane module can be indirectly checked by confirming the temperature of the fermenter during sterilization. Can be estimated. Further, the temperature of the separation membrane module 2 can be confirmed by inserting a thermocouple into the separation membrane of the separation membrane module and measuring the temperature during sterilization. Alternatively, the correlation between the housing surface temperature of the separation membrane module 2 and the internal temperature of the separation membrane module 2 is confirmed in advance. At the time of sterilization, the internal temperature of the separation membrane module 2 can be estimated by measuring the surface temperature of the casing of the separation membrane module with a surface thermometer or the like. In this way, it can be confirmed whether a predetermined steam sterilization temperature has been reached.
- Whether various conditions such as the temperature and time of steam sterilization are appropriate can be determined by confirming in advance whether sterilization is performed under those conditions.
- This prior confirmation can be performed as follows. That is, steam sterilization is performed after arranging some microorganisms in a place where the temperature of the separation membrane module 2 is difficult to rise (for example, a narrow place such as between separation membranes). Thereafter, for example, a medium containing a nutrient source is supplied to the separation membrane module, and it is confirmed whether or not sterilization is properly performed by confirming whether or not the microorganisms grow.
- the separation membrane module 2 may be preheated in order to reduce the thermal load on each member of the separation membrane module.
- the separation membrane module 2 can be preheated by supplying warm water to the separation membrane module 2 via the liquid supply line 31 for supplying the liquid to be sealed.
- the hot water may be supplied through a pipe 23 or the like that supplies the stock solution.
- the temperature of the hot water supplied to the separation membrane module 2 is preferably 40 to less than 100 ° C. Preheating by supplying hot water can shorten the heating time when supplying steam to raise the temperature of the separation membrane module 2 to 121 ° C. or higher, which is a typical sterilization temperature for steam sterilization.
- the temperature of the hot water to be supplied is more preferably 80 to less than 100 ° C.
- the supply temperature of the warm water may be gradually increased. For example, the supply of warm water may be started at 20 ° C., and the temperature of the warm water may be gradually increased to about 80 ° C.
- the separation membrane module 2 having the shape there is a portion where the water vapor hardly spreads in the separation membrane module 2 having a complicated shape.
- the member of the separation membrane module 2 includes a member having low durability against a sudden temperature change, it is preferable to supply the warm water by gradually increasing the temperature of the warm water to be supplied.
- Hot water used is water that has permeated through a reverse osmosis membrane, distilled water, or ion-exchanged water heated with a heater or the like. Since it is used for sterilization, it is preferably used after sterilization, such as filter sterilization through a filter.
- the filter a commercially available sterilizing filter can be used, and a filter having a trapping diameter of about 0.2 ⁇ m is preferable.
- Water used as hot water may be stored in a tank or the like and sent to the separation membrane module 2. In this case, it can be heated to a predetermined temperature in a tank. Further, when the separation membrane module 2 is fed, a heat exchanger can be provided in the middle to heat the separation membrane module 2.
- a general heat exchanger such as a plate type, a tube type, a spiral type, or a double pipe type can also be used as the heat exchanger.
- water is supplied to the secondary side of the separation membrane module 2, and the supplied water is passed from the secondary side to the primary side. It may be liquid.
- drying of the separation membrane can be suppressed by supplying water vapor to the primary side while passing water from the secondary side to the primary side.
- warm water may be supplied from the secondary side to the primary side.
- the water supplied to the secondary side of the separation membrane module 2 is permeated to the primary side of the separation membrane module 2 and is discharged from the separation membrane module 2 through the discharge line 33 and the discharge valve 32.
- the water retained on the secondary side can be directly discharged.
- the discharge is performed by the discharge line 26 and the discharge valve 27 directly connected to the secondary side.
- the secondary membrane In the sterilization process, when water is supplied from the secondary side to the primary side and water vapor is supplied to the primary side, the secondary membrane is maintained so as to maintain a predetermined sterilization temperature during the sterilization process of the separation membrane module 2. It is preferable to control the temperature and flow rate of water supplied from the side to the primary side. When the temperature of the supplied water is low and the flow rate is high, the temperature in the vicinity of the separation membrane of the separation membrane module 2 can be lowered from a predetermined sterilization temperature by the water that permeates from the secondary side to the primary side of the separation membrane module 2. There is sex.
- the water flux supplied to the separation membrane module 2 is preferably 0.001 to 1 m / d, and more preferably 0.01 to 0.1 m / d.
- a predetermined steam sterilization temperature can be obtained while being supplied to the separation membrane and passing through the separation membrane if the flux is at this level. Therefore, there is no concern of adversely affecting the maintenance of the steam sterilization temperature.
- water can be supplied intermittently or continuously. However, it is preferable to supply water continuously in consideration of prevention of drying of the separation membrane and stability of temperature during sterilization.
- FIG. 2 is a flowchart for explaining the sterilization process of the separation membrane module 2 according to the first embodiment.
- a liquid is supplied from the liquid supply unit 40 to the primary side of the separation membrane module 2 and then passed to the secondary side (step S1).
- the liquid is separated via the liquid supply line 31 by the liquid supply pump 21 with the discharge valve 27, the supply valve 19, the drain valve 32, the filtration valve 13 and the circulation valve 17 closed and the liquid supply valve 22 opened. It is supplied to the primary side of the membrane module 2.
- the liquid is passed from the primary side to the secondary side.
- the liquid passage is preferably performed after the filtration valve 13 is opened and then suctioned from the secondary side by the filtration pump 11 until the secondary side is filled with the sealed liquid.
- the temperature of the liquid supplied by the liquid supply unit 40 may be a room temperature or a heated one.
- the secondary side is sealed to enclose the liquid on the secondary side (step S2).
- the secondary side liquid is sealed by closing the filtration valve 13. After the filtration valve 13 is closed, the sealed liquid supply pump 21 is stopped, and the supply of the liquid to the separation membrane module 2 is stopped.
- step S3 When supplying water vapor, the circulation valve 17 and the liquid supply valve 22 are closed, the supply valve 19 and the discharge valve 32 are opened, and water vapor is supplied to the primary side of the separation membrane module 2 via the pipe 34.
- the supply of water vapor by the steam supply unit 20 is continued until the separation membrane module 2 is heated to a predetermined sterilization temperature while discharging water vapor from the discharge line 33.
- the liquid filled on the primary side is discharged from the discharge line 33.
- water vapor is ventilated where there is a large amount of liquid water, a sudden temperature change occurs due to contact between the water vapor and the liquid water, and hammering occurs. It may be discharged before. Since it is necessary to keep the sterilization space at or above the saturated water vapor pressure so that the predetermined temperature is obtained by steam sterilization, only the condensed water (drain) of water vapor is supplied to the discharge line 33 while maintaining the set pressure. A steam trap or the like can be provided so that it can be discharged. Further, the separation membrane module 2 and another device may be steam sterilized simultaneously, or the separation membrane module 2 can be steam sterilized alone by closing a valve in the middle of the cross-flow pipe 25.
- the separation membrane module 2 is sterilized at a predetermined sterilization temperature for a predetermined time (step S4).
- the sterilization temperature is usually 121 ° C. and the sterilization time is 15 to 20 minutes.
- the sterilization temperature and sterilization time are appropriately set. It may be changed.
- an amount of water vapor that supplements the heat radiation of each part of the separation membrane module 2 is supplied. It is also preferable to reduce the supply of water vapor by keeping each part of the sterilization apparatus 100 warm.
- the combination of the temperature increase in step S3 and the temperature maintenance in step S4 can be regarded as a sterilization process.
- the water vapor on the primary side of the separation membrane module 2 and the liquid sealed on the secondary side are discharged to complete the sterilization process (step S5).
- the primary water vapor and the liquid sealed on the secondary side may be discharged through the discharge lines 26 and 33.
- the separation membrane module 2 may be allowed to cool to lower the primary steam pressure, or may be cooled by supplying compressed air or cooling water. Further, the liquid sealed on the secondary side may be kept sealed in order to prevent the separation membrane from drying, particularly in the case of water.
- the liquid sealed on the secondary side passes through the separation membrane by supplying water vapor to the primary side. Since heat is conducted to each part of the separation membrane module 2, the time required for the separation membrane module 2 to rise to a predetermined sterilization temperature can be greatly shortened. Also, when sterilizing separation membranes with particularly large membrane pores, water vapor may pass from the primary side to the secondary side of the separation membrane in order to sterilize with a liquid sealed on the secondary side.
- the water vapor spreads also to the secondary side, so that the secondary side can also be heated with water vapor, and the time required to raise the temperature to a predetermined sterilization temperature can be greatly shortened. Thereby, it becomes possible to suppress deterioration by heat, such as a potting agent, and the replacement frequency of the separation membrane module 2 can be reduced.
- FIG. 3 is a schematic diagram of an apparatus for sterilizing a separation membrane module according to Modification 1 of Embodiment 1 of the present invention.
- the liquid supply unit 40 is connected to the secondary side of the separation membrane module 2.
- the sterilization apparatus 100A directly supplies liquid from the liquid supply unit 40 to the secondary side of the separation membrane module 2 with the liquid supply unit 40 connected to the discharge line 26 and the discharge valve 27 closed.
- the filtration valve 13 is opened.
- the filtration is performed.
- the liquid is sealed on the secondary side.
- the liquid may be subjected to reverse pressure filtration from the secondary side to the primary side of the separation membrane.
- the liquid supply valve 22 and the like on the secondary side of the separation membrane module 2 are closed, and the liquid is sealed on the secondary side.
- the liquid supply path from the secondary side for example, the filtration valve 13, the discharge valve 27, and the liquid supply valve 22 in FIG. Is closed.
- the sterilization process may be performed while the filtration valve 13 and the discharge valve 27 are closed and the liquid supply valve 22 is opened, that is, while supplying the liquid to the secondary side.
- FIG. 4 is a schematic diagram of an apparatus for sterilizing a separation membrane module according to Modification 2 of Embodiment 1 of the present invention.
- the sterilization apparatus 100 ⁇ / b> B includes a separation membrane cleaning device 18 that supplies a cleaning liquid to the secondary side of the separation membrane module 2.
- the separation membrane cleaning device 18 includes a cleaning liquid tank, a cleaning liquid supply pump 12, and a cleaning liquid valve 14.
- the separation membrane cleaning device 18 drives the cleaning liquid supply pump 12 to supply the cleaning liquid from the cleaning liquid tank to the secondary side of the separation membrane module 2 via the cleaning liquid supply line 29.
- the separation membrane cleaning device 18 uses the secondary side of the separation membrane module 2.
- the liquid remaining on the primary side and the secondary side of the separation membrane module 2 can be washed with the supplied washing liquid.
- the separation membrane cleaning device 18 may be connected to the primary side, and the cleaning liquid may be supplied from the primary side to the secondary side.
- the cleaning may be performed by supplying a cleaning liquid to the primary side of the separation membrane module 2 and filtering the secondary side of the separation membrane.
- water can be suitably used, but water to which an alkali, an acid, an oxidizing agent, or a reducing agent used for back pressure cleaning of the separation membrane module 2 is added may also be used.
- the sealed liquid when a solvent other than water is used as the sealed liquid, the sealed liquid may be reused by connecting the discharge line 26 and the liquid supply unit 40. Further, in the sterilization process, the sterilization process may be performed while the filtration valve 13 and the discharge valve 27 are closed and the cleaning liquid valve 14 is opened, that is, while supplying the cleaning liquid to the secondary side.
- FIG. 5 is a schematic diagram of a membrane separation type continuous fermentation apparatus according to Embodiment 2 of the present invention.
- the membrane separation type continuous fermentation apparatus 200 includes a fermentation tank 1 that converts a fermentation raw material into a fermentation broth containing a chemical by fermentation fermentation of microorganisms, a separation membrane module 2 that separates the chemical from the fermentation broth, and a separation membrane.
- the circulation pump 8 that supplies the fermentation liquid to the module 2, the steam supply unit 20 that supplies steam for steam sterilization, the liquid supply unit 40 that supplies the sealed liquid to the secondary side of the separation membrane module 2, and the respective parts are controlled. And a control device 50.
- the membrane separation type continuous fermentation apparatus 200 includes a stirring device 4 and a gas supply device 15.
- the stirring device 4 stirs the fermentation liquid in the fermenter 1.
- the gas supply apparatus 15 can supply the required gas. At this time, the supplied gas can be recovered, recycled, and supplied again by the gas supply device 15.
- the membrane separation type continuous fermentation apparatus 200 includes a pH sensor / control apparatus 5 and a neutralizing agent supply pump 10.
- the pH sensor / control device 5 detects the pH of the culture solution, and controls the neutralizing agent supply pump 10 according to the result so that the culture solution shows a pH within the set range.
- the neutralizing agent supply pump 10 is connected to an acidic aqueous solution tank and an alkaline aqueous solution tank, and adjusts the pH of the culture solution by adding one of the aqueous solutions to the fermenter 1. Fermentative production with high productivity can be performed by maintaining the pH of the culture solution within a certain range.
- the neutralizing agent that is, the acidic aqueous solution and the alkaline aqueous solution correspond to the pH adjusting solution.
- the circulation pump 8 sends the culture solution in the apparatus, that is, the fermentation solution, from the fermentation tank 1 to the separation membrane module 2 and circulates the unfiltered fermentation solution from the separation membrane module 2 to the fermentation vessel 1 by cross flow. .
- the circulation pump 8 sends the fermentation solution to the separation membrane module 2 via the circulation valve 17 and the piping 23, and ferments the unfiltered fermentation solution that has not been filtered by the separation membrane module 2 via the piping 25.
- the fermentation liquor containing the chemical product that is the fermentation product is filtered by the separation membrane module 2 to be separated into the microorganism and the chemical product that is the fermentation product, and is taken out from the system as a filtrate.
- the microorganism concentration in the apparatus system is maintained high. As a result, highly productive fermentation production is possible.
- the separation membrane module 2 is connected to the fermenter 1 via a circulation pump 8.
- the filtration by the separation membrane module 2 is preferably performed while suctioning by the filtration pump 11.
- the filtrate filtered by the separation membrane module 2 is discharged and collected from the filtrate discharge line 24 via the filtration valve 13.
- the membrane separation type continuous fermentation apparatus 200 can include a differential pressure sensor / control device 7 that detects a differential pressure of the separation membrane of the separation membrane module 2. While detecting the differential pressure of the separation membrane of the separation membrane module 2 by the differential pressure sensor / control device 7, the filtration pump 11 is adjusted so that the differential pressure of the separation membrane of the separation membrane module 2 shows a value within a certain range. By controlling, stable filtration can be performed.
- the fermenter 1 can include a temperature control device 3.
- the temperature control device 3 includes a temperature sensor that detects temperature, a heating unit and / or a cooling unit, and a control unit.
- the temperature control device 3 detects the temperature in the fermenter 1 by a temperature sensor, and controls the heating unit and / or the cooling unit by the control unit so that the temperature shows a value within a certain range according to the detection result. Then, the temperature in the fermenter 1 is controlled.
- the microorganism concentration is kept high by maintaining the temperature of the fermenter 1 constant.
- the separation membrane module can be indirectly checked by checking the temperature of the fermenter during steam sterilization. Can also be estimated.
- water can be added to the fermenter 1 directly or indirectly.
- the water supply unit supplies water directly to the fermenter 1, and specifically includes a water supply pump 16.
- Indirect water supply includes the supply of raw materials and the addition of a pH adjusting solution.
- the substance added to the membrane separation type continuous fermentation apparatus 200 is preferably sterilized in order to prevent contamination due to contamination and perform fermentation efficiently.
- the medium may be sterilized by heating after mixing the medium raw materials.
- the water added to a culture medium, pH adjusting liquid, and a fermenter may be sterilized by passing through a filter for sterilization as needed.
- the level sensor / control device 6 includes a sensor for detecting the height of the liquid level in the fermenter 1 and a control device.
- the control device controls the amount of liquid flowing into the fermenter 1 by controlling the raw material supply pump 9, the water supply pump 16, and the like based on the detection result of the sensor, and thereby the liquid in the fermenter 1. Maintain the height of the face within a certain range.
- the separation membrane cleaning device 18 includes a cleaning liquid tank, a cleaning liquid supply pump 12, and a cleaning liquid valve 14.
- the separation membrane cleaning device 18 drives the cleaning liquid supply pump 12 to supply the cleaning liquid from the cleaning liquid tank to the secondary side of the separation membrane module 2 to perform back pressure cleaning.
- the reverse pressure cleaning is a method of removing dirt substances deposited on the surface of the separation membrane by sending the cleaning solution from the filtrate side which is the secondary side of the separation membrane to the fermentation liquid side which is the primary side. is there.
- the cleaning liquid supplied to the secondary side of the separation membrane module 2 passes through the separation membrane and is filtered to the primary side. When the cleaning liquid is supplied to the separation membrane module 2, the separation membrane is cleaned.
- the filtration valve 13 disposed between the separation membrane module 2 and the filtration pump 11 is closed, and the cleaning liquid is supplied to the separation membrane module 2 in a state where filtration in the separation membrane module 2 is stopped.
- the circulation pump 8 may be operated or stopped.
- the pressure of the cleaning liquid supply pump 12 may be set higher than the sum of the circulation pump 8 and the separation membrane differential pressure.
- alkali examples include sodium hydroxide and calcium hydroxide.
- examples of the acid include oxalic acid, citric acid, hydrochloric acid, nitric acid and the like.
- examples of the oxidizing agent include hypochlorite and hydrogen peroxide.
- examples of the reducing agent include inorganic reducing agents such as sodium bisulfite, sodium sulfite, and sodium thiosulfate.
- the transmembrane pressure difference when the fermentation solution of microorganisms or cultured cells is filtered through the separation membrane in the separation membrane module 2 may be any condition as long as the microorganisms, cultured cells, and medium components are not easily clogged.
- the filtration can be performed with the transmembrane pressure difference in the range of 0.1 kPa to 20 kPa.
- the transmembrane pressure difference is preferably in the range of 0.1 kPa to 10 kPa, more preferably in the range of 0.1 kPa to 5 kPa.
- the occurrence of problems in continuous fermentation operation is effectively suppressed by suppressing clogging of microorganisms (particularly prokaryotes) and medium components, and the decrease in the amount of permeated water. be able to.
- the steam supply unit 20 supplies water vapor to the fermenter 1, the separation membrane module 2, and the surrounding piping via the supply valve 19. Water vapor is supplied to each part of the membrane separation type continuous fermentation apparatus 200 by the supply valve 19, and the apparatus is sterilized under predetermined steam sterilization conditions. After steam sterilization, the compressed air can be supplied to the membrane separation type continuous fermentation apparatus 200 via the gas supply valve 30, and the steam can be discharged from the fermenter 1 or the like for cooling.
- FIG. 6 is a flowchart for explaining the sterilization process of the separation membrane module 2 according to the second embodiment.
- the liquid to be sealed on the secondary side by the liquid supply unit 40 is supplied to the primary side of the separation membrane module 2, Liquid is passed through to the secondary side (step S11).
- the discharge valve 27, the circulation valve 17, the filtration valve 13 and the cleaning liquid valve 14 are closed, the sealed liquid supply valve 22 is opened, and the primary of the separation membrane module 2 through the liquid supply line 31 by the sealed liquid supply pump 21.
- the filtration valve 13 is opened and the filtration pump 11 is operated to perform filtration, and the liquid is passed through the secondary side of the separation membrane module 2.
- Filtration to the secondary side is performed until the liquid to be filled is filled with 70% or more of the space surrounded by the portion of the separation membrane that is subjected to filtration on the secondary side, that is, the secondary side volume of the filtration portion. .
- the time until 70% or more of the secondary volume is satisfied is confirmed by performing a test in advance.
- the secondary side is sealed to enclose the liquid on the secondary side (step S12).
- the liquid supply pump 21, the cleaning liquid supply pump 12, and the filtration pump 11 are stopped to stop the supply of liquid to the separation membrane module 2, and the filtration valve 13, the cleaning liquid valve 14, the sealed liquid supply valve 22, and the discharge valve 27 are set.
- the liquid is sealed on the secondary side of the separation membrane module 2 by closing.
- the steam supply unit 20 supplies water vapor to the primary side of the separation membrane module 2 and each part of the membrane separation type continuous fermentation apparatus 200 such as the fermenter 1 to separate them.
- Each part of the membrane separation type continuous fermentation apparatus 200 including the membrane module 2 is heated to a predetermined sterilization temperature (step S13).
- the supply valve 19 and the circulation valve 17 are opened, and the fermenter 1 from the steam supply unit 20 is opened.
- the steam is supplied to the separation membrane module 2 and the like to raise the temperature of the separation membrane module 2.
- the condensed water (drain) generated when the membrane-separated continuous fermentation apparatus 200 is steam sterilized may be discharged from the discharge line 33 with the discharge valve 32 opened, for example.
- the discharge line 33 may be provided with a steam trap or the like.
- each part of the membrane separation type continuous fermentation apparatus 200 including the separation membrane module 2 is sterilized at a predetermined sterilization temperature for a predetermined time (step S14).
- the steam supply temperature is set to be equal to or higher than a predetermined steam sterilization temperature even in a place where the temperature is most difficult to rise.
- the steam supply time is set so that the temperature is higher than a predetermined steam sterilization temperature in a place where the temperature is most difficult to rise, and then the predetermined steam sterilization time is exceeded.
- heat dissipation measures are taken such as heat retention, but the temperature of the supplied water vapor is preferably 121 ° C. or higher.
- Step S15 the gas supply valve 30 is opened, and compressed air is supplied to each part of the membrane separation type continuous fermentation apparatus 200 including the primary side of the separation membrane module 2 to cool the membrane separation type continuous fermentation apparatus 200.
- Step S15 It may be allowed to cool naturally without supplying gas, but if a member with insufficient heat resistance is used, the service life will be shortened, cooling will be partially advanced, and part will be on the decompression side. In order to prevent this, it is preferable to cool by supplying compressed air.
- the blowing may be performed in a state where a liquid is sealed on the secondary side of the separation membrane module 2.
- step S16 After the separation membrane module 2 is cooled, the liquid sealed on the secondary side is discharged as necessary to end the sterilization process (step S16).
- the liquid sealed on the secondary side is steam.
- the heat is conducted to each part of the separation membrane module 2 through the separation membrane. Therefore, the time required for the separation membrane module 2 to rise to a predetermined sterilization temperature can be greatly shortened, and deterioration of the potting agent or the like due to heat can be suppressed.
- water vapor may pass from the primary side to the secondary side of the separation membrane. Since water vapor flows from the side to the secondary side and water vapor spreads to the secondary side as well, it can be heated from the secondary side with water vapor, and the time required to raise the temperature to a predetermined sterilization temperature can be greatly shortened.
- separation is performed by allowing the membrane separation type continuous fermentation apparatus 200 to cool by supplying compressed air after the sterilization process so that a negative pressure is not generated in a state where the liquid is sealed on the secondary side. Drying of the pores of the separation membrane in the membrane module 2 can be suppressed. Thereby, after a sterilization process, a filtration process can be rapidly performed without performing extra processes, such as liquid phase substitution of a separation membrane.
- the membrane separation type continuous fermentation apparatus 200 is allowed to cool by supplying compressed air after sterilization in a state where a liquid is sealed on the secondary side, hammering by rapid condensation of water vapor, In addition, contamination with germs can be suppressed.
- FIG. 7 is a schematic diagram of a membrane separation type continuous fermentation apparatus 200A according to a modification of the second embodiment.
- water is sealed on the secondary side of the separation membrane module 2 as follows. First, after water is supplied to the fermenter 1 by driving the water supply pump 16, the water in the fermenter 1 is circulated to the separation membrane module 2 by the circulation pump 8.
- the filtration valve 13 is opened and the filtration pump 11 is operated to perform filtration, and water is passed through the secondary side of the separation membrane module 2.
- the secondary pump can be filled with water by stopping the filtration pump 11 and closing the filtration valve 13.
- the water on the primary side of the fermenter 1, the pipes 23 and 25, the separation membrane module 2, etc. is discharged, and steam sterilization is performed as described in the second embodiment.
- the temperature raising time to the sterilization temperature can be shortened.
- the liquid supply unit 40 may be connected to the secondary side of the separation membrane module 2.
- the liquid supply unit 40 When the liquid supply unit 40 is connected to the secondary side of the separation membrane module 2, it may be connected to the filtrate discharge line 24 to which the separation membrane cleaning device 18 is connected.
- the liquid supply unit 40 When the liquid supply unit 40 is connected to the secondary side, in addition to supplying the liquid to the secondary side by the liquid supply unit 40 and sealing the liquid to the secondary side, the membrane separation type by compressed air after completion of steam sterilization
- the liquid may be continuously supplied from the liquid supply unit 40 to the separation membrane module 2 and may be cooled while performing reverse pressure filtration from the secondary side to the primary side.
- microorganisms and cultured cells used in the membrane separation type continuous fermentation apparatus 200 will be described.
- the microorganisms and cultured cells used in the present embodiment are not particularly limited.
- yeasts such as baker's yeast often used in the fermentation industry
- eukaryotic cells such as filamentous fungi, E. coli, lactic acid bacteria, coryneform bacteria and Examples include prokaryotic cells such as actinomycetes.
- Examples of cultured cells include animal cells and insect cells.
- the microorganisms and cultured cells used may be those isolated from the natural environment, or may be those whose properties have been partially modified by mutation or genetic recombination.
- yeast When producing lactic acid, it is preferable to use yeast for eukaryotic cells and lactic acid bacteria for prokaryotic cells. Among these, yeast in which a gene encoding lactate dehydrogenase is introduced into cells is preferable.
- lactic acid bacteria are preferably lactic acid bacteria that produce 50% or more lactic acid as a yield to sugar relative to glucose consumed, and more preferably 80% or more as a yield against sugar. is there.
- the fermentation raw material used in the present embodiment may be any material that can promote the growth of the microorganisms to be cultured and the cultured cells and can satisfactorily produce a chemical product that is the target fermentation product.
- a liquid medium is used as a fermentation raw material.
- a substance that is a component in a medium and is converted into a target chemical (that is, a raw material in a narrow sense) is sometimes referred to as a raw material, but in this document, the entire medium is referred to as a raw material unless otherwise distinguished.
- the narrowly defined raw materials are sugars such as glucose, fructose, and sucrose, which are fermentation substrates for obtaining alcohol as a chemical product, for example.
- the raw material appropriately contains a carbon source, a nitrogen source, inorganic salts, and, if necessary, organic micronutrients such as amino acids and vitamins.
- a carbon source sugars such as glucose, sucrose, fructose, galactose and lactose, starch saccharified solution containing these sugars, sweet potato molasses, sugar beet molasses, high test molasses, organic acids such as acetic acid, alcohols such as ethanol, And glycerin and the like are used.
- Nitrogen sources include ammonia gas, aqueous ammonia, ammonium salts, urea, nitrates, and other supplementary organic nitrogen sources such as oil cakes, soybean hydrolysates, casein degradation products, other amino acids, vitamins, Corn steep liquor, yeast or yeast extract, meat extract, peptides such as peptone, various fermented cells and hydrolysates thereof are used.
- supplementary organic nitrogen sources such as oil cakes, soybean hydrolysates, casein degradation products, other amino acids, vitamins, Corn steep liquor, yeast or yeast extract, meat extract, peptides such as peptone, various fermented cells and hydrolysates thereof are used.
- inorganic salts phosphates, magnesium salts, calcium salts, iron salts, manganese salts and the like may be added.
- the raw material may contain an antifoaming agent as necessary.
- the culture solution is a solution obtained as a result of growth of microorganisms or cultured cells as fermentation raw materials.
- fermentation raw materials can be added to the culture solution, but the composition of the additional fermentation raw materials can be changed as appropriate from the composition at the start of the culture so that the productivity of the target chemical product is increased. Also good.
- the concentration of the fermentation raw material in a narrow sense, the concentration of other components in the medium, and the like can be changed.
- a fermentation liquid is a liquid containing the substance produced as a result of fermentation, and may contain raw materials, microorganisms or cultured cells, and chemicals.
- culture solution and “fermentation solution” are sometimes used interchangeably.
- a chemical product that is, a substance after conversion is produced in the fermentation broth by the microorganism or the cultured cell.
- the chemicals include substances that are mass-produced in the fermentation industry, such as alcohols, organic acids, amino acids, and nucleic acids.
- alcohols include ethanol, 1,3-butanediol, 1,4-butanediol, glycerol, and the like.
- organic acids include acetic acid, lactic acid, pyruvic acid, succinic acid, malic acid, itaconic acid, and citric acid
- nucleic acids include inosine, guanosine, and cytidine. It is also possible to apply the method of the invention to the production of substances such as enzymes, antibiotics and recombinant proteins.
- the membrane separation type continuous fermentation apparatus 200 can be applied to the production of chemical products, dairy products, pharmaceuticals, foods or brewed products.
- the chemical product include organic acids, amino acids, and nucleic acids.
- the dairy product include low-fat milk.
- the food include lactic acid beverages. , Beer and shochu.
- enzymes, antibiotics, recombinant proteins and the like produced by the production method of the present invention can be applied to pharmaceutical products.
- continuous fermentation that is, withdrawal of the culture solution
- continuous fermentation may be started after batch culture or fed-batch culture is performed at the initial stage of culture to increase the microorganism concentration.
- a high concentration of cells may be seeded and continuous fermentation may be performed at the start of the culture.
- chemicals by continuous fermentation it is possible to supply a raw material culture solution and extract a culture from an appropriate time.
- the starting times of the supply of the raw material culture solution and the extraction of the culture solution are not necessarily the same.
- the supply of the raw material culture solution and the withdrawal of the culture solution may be continuous or intermittent.
- Nutrients necessary for cell growth may be added to the culture solution so that cell growth can be performed continuously. Maintaining a high concentration of microorganisms or cultured cells in the culture solution as long as the environment of the culture solution is not appropriate for the growth of microorganisms or cultured cells and does not increase the rate of death, efficient productivity This is a preferred embodiment for obtaining.
- the concentration of microorganisms or cultured cells in the culture solution in D-lactic acid fermentation using SL lactic acid bacteria, good production efficiency can be obtained by maintaining the microorganism concentration at 5 g / L or more as the dry weight.
- the saccharide concentration in the culture solution is preferably maintained at 5 g / L or less.
- the reason why it is preferable to maintain the saccharide concentration in the culture solution at 5 g / L or less is to minimize the loss of saccharide due to withdrawal of the culture solution.
- Microorganisms and cultured cells are usually cultured at a pH of 3 to 8 and a temperature of 20 ° C. to 60 ° C.
- the pH of the culture solution is usually adjusted to a predetermined value of 3 or more and 8 or less with an inorganic acid or an organic acid, an alkaline substance, urea, calcium carbonate, ammonia gas, or the like. If it is necessary to increase the oxygen supply rate, means such as adding oxygen to the air to keep the oxygen concentration at 21% or higher, pressurizing the culture solution, increasing the stirring rate, or increasing the aeration rate can be used. .
- Microbial concentration can be measured by taking a sample and measuring it.
- a microbial concentration sensor such as an MLSS measuring device in the microbial fermenter and continuously monitor the change of the microbial concentration.
- the culture solution, microorganisms or cultured cells can be extracted from the fermenter as necessary. For example, if the concentration of microorganisms or cultured cells in the fermenter becomes too high, the separation membrane is likely to be clogged. Moreover, although the production performance of a chemical may change depending on the concentration of microorganisms or cultured cells in the fermenter, the production performance can be maintained by extracting the microorganisms or cultured cells using the production performance as an index.
- the continuous culture operation performed while growing fresh cells with fermentation production capacity is a continuous culture method that produces products while growing cells, the number of fermenters Does not matter.
- the continuous culture operation is usually performed in a single fermenter for culture management. It is also possible to use a plurality of fermenters because the fermenter has a small capacity. In this case, even if continuous culture is performed using a plurality of fermenters connected in parallel or in series by piping, high productivity of the fermentation product can be obtained.
- This membrane-forming stock solution was uniformly applied to the surface of a hollow fiber membrane having a spherical structure, and immediately solidified in a water bath to produce a hollow fiber membrane having a three-dimensional stitch structure formed on the spherical structure layer.
- the average pore diameter of the treated water side surface of the obtained hollow fiber membrane was 0.05 ⁇ m.
- Reference Example 2 Production of Separation Membrane Module 2
- a separation membrane module 2 was produced using a molded product which is a polysulfone resin cylindrical container (inner diameter 35 mm) for the separation membrane module case.
- the hollow fiber membrane produced in Reference Example 1 was used as a separation membrane, and contacted with saturated steam at 121 ° C. for 1 hour.
- an autoclave “LSX-700” manufactured by TOMY was used for contact with saturated water vapor.
- the separation membrane module 2 has a structure having a separation membrane module 2 horizontal lower nozzle, a separation membrane module 2 horizontal upper nozzle, and nozzles at the upper end and lower end of the separation membrane module 2, respectively.
- fluid is flowed into / out of the hollow fiber membrane from the separation membrane module lower nozzle or upper nozzle.
- An 80% ethanol aqueous solution was supplied to the primary side of the module case, a part was filtered from the secondary side, the inside of the separation membrane module 2 was filled with the 80% ethanol aqueous solution, and allowed to stand for 1 hour. Thereafter, an 80% ethanol aqueous solution was discharged, and the inside of the separation membrane module 2 was washed and replaced with distilled water.
- the hollow fiber porous membrane was evaluated for pure water permeability, it was 3.9 ⁇ 10 ⁇ 9 m 3 / m 2 / s / Pa.
- the amount of water permeation was measured using purified water at a temperature of 25 ° C. by a reverse osmosis membrane at a head height of 1 m.
- the separation membrane module 2 was filled with water and stored.
- Example 1 The separation membrane module produced as described above was installed in a fermentation liquid circulation line of a membrane separation type continuous fermentation apparatus 200A as shown in FIG. First, 15 L of water was added to the fermenter 1, and water was circulated from the fermenter 1 to the circulation pump 8 and the separation membrane module 2 with the circulation pump 8. Thereafter, the filtration valve 13 of the separation membrane module 2 was opened and the filtration pump 11 was operated to perform filtration, and water was sealed on the secondary side of the separation membrane module 2. Filtration was performed at a filtration flux of 0.2 m / day for 30 minutes. When the water was sealed, the filtration valve 13, the cleaning liquid valve 14 and the discharge valve 27 were closed.
- This separation membrane module 2 was repeatedly steam sterilized, and there was no problem in the leak test of Reference Example 3 up to 10 steam sterilization treatments. Moreover, the pure water permeation amount of the hollow fiber membrane module after performing the steam sterilization treatment 10 times was 98% at the time of producing the hollow fiber.
- Example 1 Using the separation membrane module 2 sterilized by steam, continuous fermentation was performed using a membrane separation type continuous fermentation apparatus 200A.
- the operating conditions in Example 1 are as follows unless otherwise specified. Fermenter 1 capacity: 20 (L) Fermenter 1 effective volume: 15 (L) Fermenter 1 temperature adjustment: 37 (° C) Fermenter 1 Aeration: Nitrogen gas 2 (L / min) Fermenter 1 stirring speed: 600 (rpm) Fermenter 1 pH adjustment: adjusted to pH 6 with 3N Ca (OH) 2 Supply of lactic acid fermentation medium: Fermenter 1 liquid volume is controlled to be constant at about 15 L, and circulating liquid volume by added fermenter circulating apparatus: 10 (L / min) Membrane filtration flow rate control: Flow rate control by suction pump Intermittent filtration treatment: Periodic operation of filtration treatment (9 minutes) to filtration stop treatment (1 minute) Membrane filtration flux: 0.1 (m / day) or more 0.3 Variable so that the transmembrane pressure difference is 20 kPa or less
- the medium was used after steam sterilization under saturated steam at 121 ° C. for 20 minutes.
- Sporelactobacillus lavolacticus JCM2513 (SL strain) was used as the microorganism, the lactic acid fermentation medium having the composition shown in Table 1 was used as the medium, and the concentration of the product lactic acid was evaluated using the HPLC shown below under the following conditions: I went there.
- the optical purity of lactic acid was analyzed under the following conditions.
- the optical purity of L-lactic acid is calculated by the following formula (1).
- Optical purity (%) 100 ⁇ (LD) / (D + L) (1)
- the optical purity of D-lactic acid is calculated by the following formula (2).
- Optical purity (%) 100 ⁇ (DL) / (D + L) (2)
- L represents the concentration of L-lactic acid
- D represents the concentration of D-lactic acid.
- the SL strain was first cultured overnight in a test tube with 5 mL of lactic acid fermentation medium (pre-culture).
- the obtained culture solution was inoculated into 100 mL of a fresh lactic acid fermentation medium, and cultured with shaking in a 1000 mL Sakaguchi flask at 30 ° C. for 24 hours (pre-culture).
- the culture medium is inoculated with a culture medium in a 15 L fermenter 1 of a continuous fermenter 200A shown in FIG. 7, and the fermenter 1 is agitated by the attached agitator 4 to adjust the aeration amount of the fermenter 1.
- temperature adjustment and pH adjustment were performed, and the culture was performed for 24 hours without operating the circulation pump 8 (pre-culture).
- the circulation pump 8 is operated, the lactic acid fermentation medium is continuously supplied in addition to the operating conditions at the time of pre-culture, and the amount of membrane permeate is controlled so that the amount of fermentation liquid in the continuous fermentation apparatus is 15L Then, continuous culture was performed, and D-lactic acid was produced by continuous fermentation. The amount of permeated water permeation when performing the continuous fermentation test was controlled by the filtration pump 11 so that the filtration amount was the same as the fermentation medium supply flow rate. The produced D-lactic acid concentration and residual glucose concentration in the membrane permeation fermentation broth were measured appropriately. Periodic operation from filtration (9 minutes) to filtration stop (1 minute) was performed by intermittent filtration. By producing a chemical in the membrane separation type continuous fermentation apparatus 200A shown in FIG. 7, continuous fermentation could be performed for 400 hours.
- Example 2 As in Example 1, the separation membrane module 2 was connected to the membrane separation type continuous fermentation apparatus 200A shown in FIG. First, 10 L of water was added to the fermenter 1, and water was circulated between the fermenter 1, the circulation pump 8, and the separation membrane module 2 with the circulation pump 8. Thereafter, the filtration valve 13 of the separation membrane module 2 was opened and the filtration pump 11 was operated to perform filtration, water was filtered on the secondary side of the separation membrane, and water was sealed on the secondary side. Filtration was performed at a filtration flux of 0.1 m / day for 5 minutes. The water was sealed on the secondary side by closing the filtration valve 13, the cleaning liquid valve 14, and the discharge valve 27.
- This separation membrane module 2 was repeatedly steam sterilized, and there was no problem in the leak test of Reference Example 3 up to 10 steam sterilization treatments. Moreover, the pure water permeation amount of the hollow fiber membrane module after performing the steam sterilization treatment 10 times was 99% at the time of producing the hollow fiber.
- Example 3 Similarly to Example 1, the separation membrane module was connected to the membrane separation type continuous fermentation apparatus 200A shown in FIG. 7, and steam sterilization was performed. First, 10 L of glycerin 10 wt% aqueous solution was added to the fermenter 1, and the glycerin aqueous solution was circulated between the fermenter 1 and the separation membrane module 2 by the circulation pump 8. Thereafter, the filtration valve 13 of the separation membrane module 2 was opened, the filtration pump 11 was operated, the glycerin aqueous solution was filtered on the secondary side of the separation membrane, and the glycerin aqueous solution was sealed on the secondary side of the separation membrane. Filtration was performed at a filtration flux of 0.2 m / day for 15 minutes.
- the glycerin aqueous solution was sealed by closing the filtration valve 13, the cleaning liquid valve 14 and the discharge valve 27. Then, the glycerol aqueous solution in the primary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged
- saturated steam controlled to 125 ° C. was supplied from the steam supply unit 20 to the fermenter 1.
- water vapor was supplied to the circulation line, the circulation pump 8, the separation membrane module 2, and the like.
- a thermocouple was installed at the center of the hollow fiber membrane bundle of the separation membrane module 2, and the temperature inside the separation membrane module 2 was observed. The temperature was raised by supplying water vapor until the temperature of the thermocouple reached 123 ° C., and the separation membrane module 2 was held at 123 ° C. or higher for 20 minutes, and then the supply of water vapor was stopped to complete the sterilization process.
- the total supply of water vapor to the separation membrane module 2 was 40 minutes.
- the gas supply valve 30 is opened to supply compressed air into the separation membrane module 2 so that the separation membrane module 2 does not become negative pressure. Allowed to cool. Then, after cooling to 30 degreeC, 1 L of ethanol 30 wt% aqueous solution was added to the fermenter 1, and the ethanol aqueous solution was circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8.
- the ethanol aqueous solution on the primary side and the secondary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged.
- 10 L of water was added to the fermenter 1 and circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8.
- the filtration valve 13 of the separation membrane module 2 was opened and the filtration pump 11 was operated to perform filtration, and water was filtered to the secondary side of the separation membrane. Filtration was performed at a filtration flux of 0.2 m / day for 15 minutes.
- water on the primary side and the secondary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged, and the cleaning of the separation membrane was completed.
- This separation membrane module 2 was repeatedly steam sterilized, and there was no problem in the leak test of Reference Example 3 up to 10 steam sterilization treatments. Moreover, the pure water permeation amount of the hollow fiber membrane module after performing the steam sterilization treatment 10 times was 99% at the time of producing the hollow fiber.
- Example 4 Similarly to Example 1, the separation membrane module was connected to the membrane separation type continuous fermentation apparatus 200A shown in FIG. 7, and steam sterilization was performed. First, 10 L of water was added to the fermenter 1, and water was circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8. Thereafter, the filtration valve 13 of the separation membrane module 2 was opened, the filtration pump 11 was operated, water was filtered on the secondary side of the separation membrane module 2, and water was sealed on the secondary side of the separation membrane. Filtration was performed at a filtration flux of 0.1 m / day for 5 minutes. The water was sealed by closing the filtration valve 13, the cleaning liquid valve 14, and the discharge valve 27.
- the separation membrane module 18 was cooled by using the separation membrane cleaning device 18. Specifically, the cleaning liquid valve 14 is opened and the cleaning liquid supply pump 12 is operated, so that 80 ° C. warm water is supplied from the secondary side to the primary side of the separation membrane module at a reverse pressure filtration flux of 1 m / d. The separation membrane module was cooled by passing the solution for 5 minutes.
- Example 5 A separation membrane module (see FIG. 3) with the liquid supply unit 40 connected to the secondary side was connected to a membrane separation type continuous fermentation apparatus, and steam sterilization was performed.
- a 10 wt% aqueous solution of glycerin is supplied from the liquid supply unit 40 to the secondary side of the separation membrane module 2 by the liquid supply pump 21.
- Reverse pressure filtration was performed from the side to the primary side, and an aqueous glycerin solution was sealed on the secondary side of the separation membrane.
- Back pressure filtration was performed at a filtration flux of 0.1 m / day for 5 minutes.
- thermocouple was installed at the center of the hollow fiber membrane bundle of the separation membrane module 2, and the temperature inside the separation membrane module 2 was observed. Water vapor is supplied until the temperature of the thermocouple reaches 123 ° C. to raise the temperature of the separation membrane module 2, and after the separation membrane module 2 is held at 123 ° C. or higher for 20 minutes, the supply of water vapor is stopped, and the separation membrane module 2 The steam sterilization of was finished. The total supply of water vapor to the separation membrane module 2 was 35 minutes.
- the gas supply valve 30 is opened and compressed air is supplied into the separation membrane module 2 so that the separation membrane module 2 does not become negative pressure and is released. Chilled. Then, after cooling to 30 degreeC, 1 L of ethanol 30 mass% aqueous solution was added to the fermenter 1, and the ethanol aqueous solution was circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8.
- the ethanol aqueous solution on the primary side and the secondary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged.
- 10 L of water was added to the fermenter 1 and circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8.
- the filtration valve 13 of the separation membrane module 2 was opened and the filtration pump 11 was operated to perform filtration, and water was filtered to the secondary side of the separation membrane. Filtration was performed at a filtration flux of 0.2 m / day for 15 minutes.
- water on the primary side and the secondary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged, and the cleaning of the separation membrane was completed.
- This separation membrane module 2 was repeatedly steam sterilized, and there was no problem in the leak test of Reference Example 3 up to 10 steam sterilization treatments. Moreover, the pure water permeation amount of the hollow fiber membrane module after performing the steam sterilization treatment 10 times was 98% at the time of producing the hollow fiber.
- Example 6 The separation membrane module (see FIG. 3) with the liquid supply unit 40 connected to the secondary side is connected to the membrane separation type continuous fermentation apparatus 200A shown in FIG. went.
- 10 L of water was added to the fermenter 1, and water was circulated between the fermenter 1 and the separation membrane module 2 by the circulation pump 8.
- the filtration valve 13 of the separation membrane module 2 was opened, the filtration pump 11 was operated to filter water on the secondary side of the separation membrane, and water was sealed on the secondary side of the separation membrane. Filtration was performed at a filtration flux of 0.1 m / day for 5 minutes.
- the water was sealed by closing the filtration valve 13, the cleaning liquid valve 14, the sealed liquid supply valve 22, and the discharge valve 27.
- Saturated steam controlled to 125 ° C. is supplied from the steam supply unit 20 to the fermenter 1 while performing reverse pressure filtration as described above, and after the fermenter 1 reaches 121 ° C., the circulation line, the circulation pump 8, and the separation membrane Water vapor was supplied to module 2 and the like.
- a thermocouple was installed at the center of the hollow fiber membrane bundle of the separation membrane module 2, and the temperature inside the separation membrane module 2 was observed. Water vapor is supplied until the temperature of the thermocouple reaches 123 ° C. to raise the temperature of the separation membrane module 2, and after the separation membrane module 2 is held at 123 ° C. or higher for 20 minutes, the supply of water vapor is stopped, and the separation membrane module 2 The sterilization treatment of was finished.
- the total supply of water vapor to the separation membrane module 2 was 40 minutes. After the steam sterilization, when the temperature of the fermenter 1 drops to 100 ° C., the gas supply valve 30 is opened to supply compressed air into the separation membrane module 2 so that the separation membrane module 2 does not become negative pressure. After standing to cool, the water sealed on the secondary side of the separation membrane module 2 was discharged, and the sterilization treatment of the separation membrane module 2 was completed.
- This separation membrane module 2 was repeatedly steam sterilized, and there was no problem in the leak test of Reference Example 3 up to 10 steam sterilization treatments. Moreover, the pure water permeation amount of the hollow fiber membrane module after performing the steam sterilization treatment 10 times was 99% at the time of producing the hollow fiber.
- Example 7 Similarly to Example 1, the separation membrane module was connected to the membrane separation type continuous fermentation apparatus 200A shown in FIG. First, 10 L of water was added to the fermenter 1, and water was circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8. Thereafter, the filtration valve 13 of the separation membrane module 2 was opened, the filtration pump 11 was operated, water was filtered on the secondary side of the separation membrane module 2, and water was sealed on the secondary side of the separation membrane. Filtration was performed at a filtration flux of 0.1 m / day for 5 minutes. The water was sealed by closing the filtration valve 13, the cleaning liquid valve 14, and the discharge valve 27. Thereafter, water on the primary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged.
- saturated steam controlled at 125 ° C. was supplied from the steam supply unit 20 to the fermenter 1.
- water vapor was supplied to the circulation line, the circulation pump 8, the separation membrane module 2, and the like.
- a thermocouple was installed at the center of the hollow fiber membrane bundle of the separation membrane module 2, and the temperature inside the separation membrane module 2 was observed. Water vapor is supplied until the temperature of the thermocouple reaches 123 ° C. to raise the temperature of the separation membrane module 2, and after the separation membrane module 2 is held at 123 ° C. or higher for 20 minutes, the supply of water vapor is stopped, and the separation membrane module 2 The steam sterilization of was finished.
- the membrane After completion of steam sterilization, the membrane was allowed to cool until the surface temperature of the separation membrane module 2 reached 100 ° C. Thereafter, warm water at 80 ° C. was passed through the separation membrane module from the secondary side to the primary side at a reverse pressure filtration flux of 1 m / d for 5 minutes to cool the separation membrane module 2. After cooling the separation membrane module 2, the water sealed on the secondary side was discharged, and the sterilization treatment of the separation membrane module 2 was completed. The supply of water vapor to the separation membrane module 2 was 30 minutes in total. This separation membrane module 2 was repeatedly steam sterilized, and there was no problem in the leak test of Reference Example 3 up to 10 steam sterilization treatments. Moreover, the pure water permeation amount of the hollow fiber membrane module after performing the steam sterilization treatment 10 times was 98% at the time of producing the hollow fiber.
- Example 1 Similarly to Example 1, the separation membrane module was connected to the membrane separation type continuous fermentation apparatus 200A shown in FIG. 7, and steam sterilization was performed. First, 1 L of water was added to the fermenter 1, and water was circulated between the fermenter 1 and the separation membrane module 2 with the circulation pump 8. Thereafter, the filtration valve 13 of the separation membrane module 2 was opened and the filtration pump 11 was operated to perform filtration, and water was sealed on the secondary side of the separation membrane. Thereafter, water on the primary side of the fermenter 1, the circulation line, and the separation membrane module 2 was discharged. Moreover, the filtration valve 13 and the discharge valve 27 were opened, and the water in the secondary side of the separation membrane was discharged.
- the supply of water vapor is stopped, and the separation membrane module 2
- the sterilization treatment of was finished.
- the gas supply valve 30 is opened from the time when the temperature of the fermenter 1 drops to 100 ° C., and compressed air is supplied into the separation membrane module 2 so that the separation membrane module 2 does not become negative pressure. And cooled.
- the total supply of water vapor to the separation membrane module 2 was 55 minutes.
- This separation membrane module 2 was repeatedly steam sterilized, and the number of steam sterilization treatments was the fourth, and the leak test of Reference Example 3 failed.
- the pure water permeation amount of the hollow fiber membrane module having the third steam sterilization treatment was 90% at the time of producing the hollow fiber.
- the separation membrane module sterilization method, the chemical production method by continuous fermentation, the separation membrane module sterilization apparatus and the membrane separation type continuous fermentation apparatus of the present invention are useful for the production of chemical products that are fermentation products by microorganisms and the like. is there.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Manufacturing & Machinery (AREA)
- Inorganic Chemistry (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
本発明の実施の形態1に係る分離膜モジュールの滅菌方法について、図1を参照して説明する。図1は、本発明の実施の形態1にかかる分離膜モジュールの滅菌用装置の概略図である。滅菌用装置100は、分離膜モジュール2の1次側に水蒸気を供給する蒸気供給部20と、分離膜モジュール2の2次側に大気圧下での沸点が80℃以上の液体を供給する液体供給部40と、を備える。分離膜モジュール2には、処理対象である原液を供給する、循環バルブ17および配管23が1次側に接続されるとともに、分離膜で濾過された濾過液を分離膜モジュール2外に排出する濾過液排出ライン24が2次側に接続されている。濾過液排出ライン24には、濾過ポンプ11および濾過バルブ13が設置され、濾過バルブ13を開とし、濾過ポンプ11により吸引することにより1次側から2次側に濾過される。2次側に濾過されなかった原液は、配管25を介してクロスフローされる。
蒸気滅菌に際し、分離膜モジュール2の2次側に、高い沸点を示す液体、例えば、大気圧下での沸点が80℃以上の液体を封入することにより、封入された液体が、分離膜モジュール2の1次側に供給された水蒸気から分離膜を介して、分離膜モジュール2の各部に熱伝導することにより、液体を封入しない場合よりも分離膜モジュール2の所定の滅菌温度までの昇温時間を短縮することができる。その結果、分離膜モジュール2への熱負荷を軽減することができる。
一般に液体は、気体よりも熱伝導率が高い(例えば、水の熱伝導率は、空気の熱伝導率および水蒸気の熱伝導率よりも大きい。)ので、分離膜モジュール2の2次側に液体を供給し、封止した後、水蒸気を供給することにより、2次側に空気または水蒸気が存在する場合に比べて、分離膜モジュール2の昇温速度が早くなる。また、封入する液体の熱容量が小さい方が昇温には有利であり、分離膜モジュール2内部の熱伝導は熱容量の大小も影響すると考えられる。
2次側に液体を封入しないで、水蒸気を通気する場合、長時間の蒸気滅菌を行うと、分離膜孔中の水分が、蒸気滅菌中に飽和水蒸気と接触することで、平衡状態となる。その結果、分離膜孔中の水分が徐々に少なくなり、分離膜が乾燥する懸念があった。また、水蒸気の通気は中空糸膜を一様に透過するとは限らないので、水蒸気を通気する前に中空糸膜の2次側に存在していた空気の一部が残り、空気がロックされた状態(すなわちエアロックが生じた状態)で滞留する懸念があった。
また、逆圧濾過すること、つまり2次側に液体を供給し、2次側から1次側に分離膜を介して液体を通過させることも、本発明の「封入」および「封止」に該当する。詳細は後述する。
分離膜モジュール2の1次側に封入する液体である水を通液し、分離膜モジュール2の1次側を水で満たした後に、分離膜モジュール2の1次側に圧をかけて、または2次側から吸引して、1次側から2次側にろ過をして、2次側を水で満たした後、排出バルブ27および濾過バルブ13を閉止して、2次側を水で封入する。
分離膜モジュール2の1次側から2次側にろ過により水を供給する場合、ろ過の時間が短いと、分離膜の2次側が十分水で封入されない懸念があるため、一定時間以上ろ過をすることが好ましい。
例えば、有効長が1mの中空糸膜モジュールでは、中空糸膜の1次側を水で満たした後、0.2m/dのろ過流束で、15分以上ろ過をすると、中空糸膜のろ過部分の2次側容積に対し、90%以上を水で満たすことができる。
例えば、分離膜の1次側から2次側にろ過をした後、2次側に設置したバルブを閉止して、2次側に水を封入する。その後、分離膜の1次側の水を排出してから、2次側に設置したバルブを開けて、必要に応じ2次側を空気で加圧するなどして、2次側に封入した水を排出し、計量する。この場合、2次側の送液ラインなどを満たしていた水も含まれることになるが、事前に送液ラインの水量を計量しておけば、中空糸膜の2次側の水量を求めることができる。
または、分離膜の1次側から観察を行い、水の封入した部分と気体が残っている部分の長さを測定することで、水の封入率を求めることができる。分離膜の全体について観察できることが望ましいが、目視できない部分もあるので、部分的に観察して代表させることでも良い。
分離膜モジュール2の1次側に水蒸気を通気して蒸気滅菌する場合、1次側に水を満たした状態で水蒸気を通気すると、局所的に液体の水と水蒸気が急激な熱交換をして、1次側に存在する水が蒸発したり、水蒸気が凝縮するなどして、分離膜が振動し、分離膜モジュール2の分離膜や部材が破損する懸念がある。そのため、分離膜モジュール2の1次側に水蒸気を通気して滅菌する場合は、分離膜モジュール2の1次側に液体の水が少ないことが好ましい。
ところが、分離膜モジュール2の1次側に液体の水がなく、1次側における圧力が2次側における圧力より小さい場合は、2次側に封入した水が1次側へ逆流する可能性がある。そのため、封入の方法は特に限定されないが、例えば、2次側の送液ラインに設けた排出バルブ27等の閉止に加え、1次側の圧力が2次側の圧力より低下しないようにして、2次側の水の封入量が70%以上に保持されることが必要である。
滅菌時に分離膜モジュールの温度が適切な値まで向上しているかどうかは、以下のようにして判断することができる。
例えば、事前に蒸気滅菌時の発酵槽1の温度と分離膜モジュール2の温度の相関関係を確認しておくことにより、滅菌時に、発酵槽の温度を確認することで、間接的に分離膜モジュールの温度を推定することができる。
また、分離膜モジュールの分離膜の中に熱電対を挿入し、滅菌時に温度を測定することで、分離膜モジュール2の温度を確認することもできる。
もしくは、事前に分離膜モジュール2の筐体表面温度と分離膜モジュール2の内部温度との相関関係を確認しておく。滅菌時には、分離膜モジュールの筐体の表面温度を、表面温度計などで測定することで、分離膜モジュール2の内部温度を推定することができる。こうして、所定の蒸気滅菌温度に到達しているか確認することができる。
なお、蒸気滅菌の温度および時間等の諸条件が適切かどうかは、その条件下で滅菌ができているかを事前に確認することで、判断できる。この事前確認は、次のようにして行うことができる。すなわち、分離膜モジュール2における温度が上昇しにくい箇所(例えば分離膜の間などの狭い場所)に、なんらかの微生物を配置した後、蒸気滅菌を行う。その後、例えば栄養源を含む培地を分離膜モジュールに供給し、微生物が増殖するか確認を行うことで、適切に滅菌できているか確認することができる。
例えば、封入する液体を供給する液体供給ライン31等を介して分離膜モジュール2に温水を供給することで、分離膜モジュール2を予熱することができる。温水は、原液を供給する配管23等を介して供給しても良い。分離膜モジュール2に供給する温水の温度は、40~100℃未満であることが好ましい。温水供給による予熱により、一般的な蒸気滅菌の滅菌温度である121℃以上まで、水蒸気を供給して分離膜モジュール2を昇温する際に、昇温時間を短くすることができる。供給する温水の温度は、より好ましくは、80~100℃未満である。温水の供給温度を徐々に上げてもよく、例えば、20℃で温水の供給を開始し、徐々に温水の温度を80℃程度まで昇温しても良い。
分離膜モジュール2の部材に急激な温度変化に対して耐久性が低いものを含む場合は、供給する温水の温度を徐々に上げて温水を供給することが好ましい。
温水は逆浸透膜を透過させた水や蒸留水、イオン交換水をヒーター等で加熱したものを用いる。滅菌に使用することから、フィルターを透過させてフィルター滅菌する等、滅菌してから使用することが好ましい。なお、フィルターは市販の滅菌用フィルターを使用することができ、捕捉径が0.2μm程度のものが好ましい。
また、滅菌後の冷却工程において、2次側から1次側に温水を供給してもよい。2次側から1次側に温水を供給することにより、高温となったポッティング層を徐々に冷却できるため、急激な冷却によるヒートショックを抑制でき、ポッティング層の劣化を抑制することができる。
分離膜モジュール2の2次側に供給された水は、分離膜モジュール2の1次側に透過され、排出ライン33および排出バルブ32を介して分離膜モジュール2から排出される。
分離膜モジュールの様式によっては、2次側に保持している水を直接排出することもできる。この場合は、2次側に直結している排出ライン26、排出バルブ27にて排出を行う。
また、水の供給は間欠、または連続で供給することができるが、分離膜の乾燥防止と滅菌時の温度の安定性とを考慮すると、連続で供給することが好ましい。
ここで、液体の水が多量にあるところに、水蒸気を通気すると、水蒸気と液体の水の接触により、急激な温度変化が発生し、ハンマリングが発生するため、1次側の水は水蒸気通気前に、排出しても良い。
なお、蒸気滅菌で所定の温度になるように、滅菌空間を飽和水蒸気圧以上に保つ必要があることから、排出ライン33には、設定の圧を保持しながら水蒸気の凝縮水(ドレン)のみを排出できる様に、スチームトラップなどを設けることができる。
また、分離膜モジュール2と他の装置とを同時に蒸気滅菌しても良いし、クロスフローの配管25の途中のバルブを閉止することで、分離膜モジュール2を単独で蒸気滅菌することもできる。
ステップS3による昇温と、ステップS4による温度維持とを合わせて、滅菌工程と見なすことができる。
なお、蒸気滅菌後は、蒸気滅菌の対象物の内部が陰圧状態になると、外気などから未滅菌物が混入(吸入)する懸念があることから、できるだけ陰圧状態を避けることが好ましい。そのため、蒸気滅菌後に滅菌水や滅菌空気などを供給して、蒸気滅菌の対象物を陽圧にすることが好ましい。
液体供給部40により、2次側に供給された液体の充填率、すなわち2次側のろ過に供される部分で囲まれた空間への液体の充填率が70%以上となった後、濾過バルブ13および液体供給バルブ22を閉止することにより、2次側に液体が封入される。あるいは、2次側に液体を満たした後、分離膜の2次側から1次側へ液体を逆圧濾過してもよい。分離膜の2次側から1次側へ液体を濾過した後、分離膜モジュール2の2次側の液体供給バルブ22等を閉止すれば、2次側に液体を封入した状態となる。
次に、本発明の実施の形態2について図5を参照して説明する。図5は、本発明の実施の形態2にかかる膜分離型連続発酵装置の概略図である。
また、蒸気滅菌時の発酵槽1の温度と分離膜モジュール2の温度の相関を事前に確認しておくことにより、蒸気滅菌時に、発酵槽の温度を確認することで、間接的に分離膜モジュールの温度を推定することもできる。
ここで、膜分離型連続発酵装置200を蒸気滅菌する際に発生する凝縮水(ドレン)は、例えば、排出バルブ32を開とし、排出ライン33から排出しても良い。この時、水蒸気の圧を一定に保持するため、排出ライン33には、スチームトラップなどを設けても良い。
中空糸膜の作製
重量平均分子量41.7万のフッ化ビニリデンホモポリマーとγ-ブチロラクトンとを、それぞれ38質量%と62質量%の割合で170℃の温度で溶解した。この高分子溶液をγ-ブチロラクトンを中空部形成液体として随伴させながら口金から吐出し、温度20℃のγ-ブチロラクトン80質量%水溶液からなる冷却浴中で固化して球状構造からなる中空糸膜を作製した。次いで、質量平均分子量28.4万のフッ化ビニリデンホモポリマーを14質量%、セルロースアセテートプロピオネート(イーストマンケミカル社製、CAP482-0.5)を1質量%、N-メチル-2-ピロリドンを77重量%、ポリオキシエチレンヤシ油脂肪酸ソルビタン(三洋化成株式会社、商品名イオネット(登録商標)T-20C)を5質量%、水を3質量%の割合で95℃の温度で混合溶解して高分子溶液を調製した。この製膜原液を、球状構造からなる中空糸膜の表面に均一に塗布し、すぐに水浴中で凝固させて球状構造層の上に三次元編目構造を形成させた中空糸膜を作製した。
得られた中空糸膜の被処理水側表面の平均細孔径は、0.05μmであった。
分離膜モジュール2の作製
分離膜モジュールケースにはポリスルホン樹脂製筒状容器(内径35mm)である成型品を用いて分離膜モジュール2を作製した。分離膜として参考例1で作製した中空糸膜を用い、121℃の飽和水蒸気と1時間接触させた。飽和水蒸気との接触には、TOMY社製のオートクレーブ「LSX-700」を使用した。中空糸膜325本(外径1.4mm、有効長20cm)を前記モジュールケース内に挿入し、ウレタン樹脂(サンユレック社製、SA-7068A/SA-7068B、2剤を重量比が64:100となるように混合)を用いてモジュールケースと中空糸膜の両端を接着した。分離膜モジュール2の中空糸膜の両端は中空糸膜を開口させるために、余分な接着部は切り落として用いた。分離膜モジュール2における中空糸膜の充填率は50%であった。なお、分離膜モジュール2は、分離膜モジュール2横下部ノズルと分離膜モジュール2横上部ノズル、さらに分離膜モジュール2上端と下端に各々ノズルを持つ構造であり、中空糸膜の内側に、分離膜モジュール上端または下端から流体を流入/排出する。また中空糸膜の外側には、分離膜モジュール横下部ノズルまたは上部ノズルから流体を流入/排出する。モジュールケースの1次側にエタノール80%水溶液を供給し、一部を2次側から濾過して、分離膜モジュール2内をエタノール80%水溶液で満たし、1時間静置した。その後、エタノール80%水溶液を排出し、蒸留水で分離膜モジュール2内を洗浄、置換した。
次に、上記の中空糸多孔性膜について純水透水量を評価したところ、3.9×10-9m3/m2/s/Paであった。透水量の測定は、逆浸透膜による25℃の温度の精製水を用い、ヘッド高さ1mで行った。分離膜モジュール2は内部に水を満たして保管した。
参考例2の通り作製した分離膜モジュール2の1次側に、100kPaの空気を供給し、分離膜モジュール2の1次側の水を2次側に濾過した後、分離膜モジュール2の1次側を封じ込めして、100kPaの空気で加圧状態とした。ここで、分離膜モジュール2の1次側供給ラインに圧力計を設置し、分離膜モジュール2の1次側の圧力を確認できるようにした。また分離膜モジュール2の2次側は大気開放とした。3分後、分離膜モジュール2の1次側の圧力の低下が10kPa以内ならば、合格と判断した。
上記の通り作製した分離膜モジュールを図7に示すような膜分離型連続発酵装置200Aの発酵液循環ラインに設置し、滅菌を行った。はじめに、発酵槽1に水を15L添加し、循環ポンプ8で発酵槽1から循環ポンプ8、分離膜モジュール2に水を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して濾過を行い、分離膜モジュール2の2次側に水を封入した。濾過は0.2m/dayの濾過流束で、30分間行った。水の封入に際しては、濾過バルブ13、洗浄液バルブ14および排出バルブ27を閉止した。事前に水の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、水は98%封入されていた。分離膜の2次側に水封入後、発酵槽1や循環ライン、分離膜モジュール2の1次側にある水を排出した。その後、蒸気供給部20から発酵槽1に125℃に制御された飽和水蒸気を供給した。発酵槽1が121℃になった後、配管23および25、循環ポンプ8、分離膜モジュール2などに水蒸気を供給した。分離膜モジュール2の中空糸膜束の中心部に熱電対を設置し、分離膜モジュール2内の温度を観察した。熱電対の温度が123℃になるまで水蒸気を供給して分離膜モジュール2を昇温し、分離膜モジュール2を123℃以上で20分保持した後、水蒸気の供給を停止して蒸気滅菌を終了した。蒸気滅菌終了後、発酵槽1の温度が100℃まで低下した時点から、気体供給バルブ30を開として、圧縮空気を分離膜モジュール2内へ供給し、分離膜モジュール2が陰圧にならないようにして放冷した後、分離膜モジュール2の2次側に封入された水を排出して分離膜モジュール2の滅菌処理を終了した。分離膜モジュール2への水蒸気の供給は、合計32分であった。この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が10回まで参考例3のリークテストで問題はなかった。また、蒸気滅菌処理を10回行なった後の中空糸膜モジュールの純水透水量は、中空糸作成時の98%であった。
発酵槽1容量:20(L)
発酵槽1有効容積:15(L)
発酵槽1温度調整:37(℃)
発酵槽1通気量:窒素ガス2(L/min)
発酵槽1攪拌速度:600(rpm)
発酵槽1内pH調整:3N Ca(OH)2によりpH6に調整
乳酸発酵培地供給:発酵槽1液量が約15Lで一定になる様に制御して添加
発酵液循環装置による循環液量:10(L/min)
膜濾過流量制御:吸引ポンプによる流量制御
間欠的な濾過処理:濾過処理(9分間)~濾過停止処理(1分間)の周期運転
膜濾過流束:0.1(m/day)以上0.3(m/day)以下の範囲で膜間差圧が20kPa以下となる様に可変。膜間差圧が範囲を超えて上昇し続けた場合は、連続発酵を終了した。
移動相:5mM p-トルエンスルホン酸(0.8mL/min)
反応相:5mM p-トルエンスルホン酸、20mMビストリス、0.1mM EDTA・2Na(0.8mL/min)
検出方法:電気伝導度カラム温度:45℃
カラム:TSK-gel Enantio L1(東ソー社製)
移動相:1mM硫酸銅水溶液
流速:1.0mL/分
検出方法:UV 254nm
温度:30℃
L-乳酸の光学純度は、次式(1)で計算される。
光学純度(%)=100×(L-D)/(D+L) ・・・(1)
また、D-乳酸の光学純度は、次式(2)で計算される。
光学純度(%)=100×(D-L)/(D+L) ・・・(2)
ここで、LはL-乳酸の濃度を表し、DはD-乳酸の濃度を表す。
実施例1と同様に分離膜モジュール2を図7に示す膜分離型連続発酵装置200Aに接続し、蒸気滅菌を行った。はじめに、発酵槽1に水を10L添加し、循環ポンプ8で水を発酵槽1、循環ポンプ8、および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して濾過を行い、分離膜の2次側に水を濾過し、2次側に水を封入した。濾過は0.1m/dayの濾過流束で、5分間行った。2次側への水の封入は、濾過バルブ13、洗浄液バルブ14、および排出バルブ27を閉止することにより行なった。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側にある水を排出した。事前に水の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、水は80%封入されていた。
その後、蒸気供給部20から発酵槽1に125℃に制御された飽和水蒸気を供給した。発酵槽1が121℃になった後、配管23および25、循環ポンプ8、分離膜モジュール2などに水蒸気を供給した。分離膜モジュール2の中空糸膜束の中心部に熱電対を設置し、分離膜モジュール2内の温度を観察した。熱電対の温度が123℃になるまで水蒸気を供給して分離膜モジュール2を昇温し、分離膜モジュール2を123℃以上で20分保持した後、水蒸気の供給を停止して蒸気滅菌を終了した。蒸気滅菌終了後、発酵槽1の温度が100℃まで低下した時点から、気体供給バルブ30を開として、圧縮空気を分離膜モジュール2内へ供給し、分離膜モジュール2が陰圧にならないようにして放冷した後、分離膜モジュール2の2次側に封入された水を排出して分離膜モジュール2の滅菌処理を終了した。分離膜モジュール2への水蒸気の供給は、合計35分であった。この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が10回まで参考例3のリークテストで問題はなかった。また、蒸気滅菌処理を10回行なった後の中空糸膜モジュールの純水透水量は、中空糸作成時の99%であった。
実施例1と同様に分離膜モジュールを図7に示す膜分離型連続発酵装置200Aに接続し、蒸気滅菌を行った。はじめに、発酵槽1にグリセリン10wt%水溶液を10L添加し、グリセリン水溶液を、循環ポンプ8により発酵槽1および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け、濾過ポンプ11を運転して分離膜の2次側にグリセリン水溶液を濾過し、分離膜の2次側にグリセリン水溶液を封入した。濾過は0.2m/dayの濾過流束で、15分間行った。グリセリン水溶液の封入は、濾過バルブ13、洗浄液バルブ14および排出バルブ27を閉止することにより行なった。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側にあるグリセリン水溶液を排出した。事前にグリセリン水溶液の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、グリセリン水溶液は95%封入されていた。
蒸気滅菌後、発酵槽1の温度が100℃まで低下した時点から、気体供給バルブ30を開として、圧縮空気を分離膜モジュール2内へ供給し、分離膜モジュール2が陰圧にならないようにして放冷した。その後、30℃まで放冷した後、発酵槽1にエタノール30wt%水溶液を1L添加し、エタノール水溶液を循環ポンプ8で発酵槽1および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して濾過を行い、分離膜の2次側にエタノール水溶液を濾過した。濾過は0.2m/dayの濾過流束で、15分間行った。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側および2次側にあるエタノール水溶液を排出した。
引き続き、発酵槽1に水を10L添加し、循環ポンプ8で発酵槽1および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して濾過を行い、分離膜の2次側に水を濾過した。濾過は0.2m/dayの濾過流束で、15分間行った。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側および2次側にある水を排出し、分離膜の洗浄を終了した。
この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が10回まで参考例3のリークテストで問題はなかった。また蒸気滅菌処理を10回行なった後の中空糸膜モジュールの純水透水量は、中空糸作成時の99%であった。
実施例1と同様に分離膜モジュールを図7に示す膜分離型連続発酵装置200Aに接続し、蒸気滅菌を行った。はじめに、発酵槽1に水を10L添加し、循環ポンプ8で、水を発酵槽1および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して、分離膜モジュール2の2次側に水を濾過し、分離膜の2次側に水を封入した。濾過は0.1m/dayの濾過流束で、5分間行った。水の封入は、濾過バルブ13、洗浄液バルブ14、および排出バルブ27を閉止することにより行なった。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側にある水を排出した。事前に水の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、水は85%封入されていた。
その後、蒸気供給部20から発酵槽1に125℃に制御された飽和水蒸気を供給した。発酵槽1が121℃になった後、循環ライン、循環ポンプ8、分離膜モジュール2などに水蒸気を供給した。分離膜モジュール2の中空糸膜束の中心部に熱電対を設置し、分離膜モジュール2内の温度を観察した。熱電対の温度が123℃になるまで水蒸気を供給して分離膜モジュール2を昇温し、分離膜モジュール2を123℃以上で20分保持した後、水蒸気の供給を停止し、分離膜モジュール2の蒸気滅菌を終了した。蒸気滅菌終了後、分離膜モジュール2の表面温度が100℃になるまで放冷した。
その後、分離膜洗浄装置18を使用することで、分離膜モジュールの冷却を行った。具体的には、洗浄液バルブ14を開とし、洗浄液供給ポンプ12を稼働させることで、分離膜モジュールの2次側から1次側へ、80℃の温水を、逆圧濾過流束1m/dで、5分間通液し、分離膜モジュールを冷却した。分離膜モジュール2の冷却後、2次側に封入された水を排出して分離膜モジュール2の滅菌処理を終了した。分離膜モジュール2への水蒸気の供給は、合計35分であった。この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が10回まで参考例3のリークテストで問題はなかった。また蒸気滅菌処理を10回行なった後の中空糸膜モジュールの純水透水量は、中空糸作成時の98%であった。
液体供給部40が2次側に接続された分離膜モジュール(図3参照)を膜分離型連続発酵装置に接続し、蒸気滅菌を行った。はじめに、封入液体供給バルブ22を開とした状態で、液体供給部40から、液体供給ポンプ21で、グリセリン10wt%水溶液を分離膜モジュール2の2次側に供給し、分離膜モジュール2の2次側から1次側へ逆圧濾過し、分離膜の2次側にグリセリン水溶液を封入した。逆圧濾過は0.1m/dayの濾過流束で、5分間行った。グリセリン水溶液の封入に際しては、濾過バルブ13、洗浄液バルブ14、および排出バルブ27を閉止して行った。その後、分離膜モジュール2の1次側へ逆圧濾過されたグリセリン水溶液を排出した。事前にグリセリン水溶液の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、グリセリン水溶液は85%封入されていた。
その後、蒸気供給部20から発酵槽1に125℃に制御された飽和水蒸気を供給し、発酵槽1が121℃になった後、循環ライン、循環ポンプ8、分離膜モジュール2などに水蒸気を供給した。分離膜モジュール2の中空糸膜束の中心部に熱電対を設置し、分離膜モジュール2内の温度を観察した。熱電対の温度が123℃になるまで水蒸気を供給して分離膜モジュール2を昇温し、分離膜モジュール2を123℃以上で20分保持した後、水蒸気の供給を停止し、分離膜モジュール2の蒸気滅菌を終了した。分離膜モジュール2への水蒸気の供給は、合計35分であった。
引き続き、発酵槽1に水を10L添加し、循環ポンプ8で発酵槽1および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して濾過を行い、分離膜の2次側に水を濾過した。濾過は0.2m/dayの濾過流束で、15分間行った。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側および2次側にある水を排出し、分離膜の洗浄を終了した。
この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が10回まで参考例3のリークテストで問題はなかった。また蒸気滅菌処理を10回行なった後の中空糸膜モジュールの純水透水量は、中空糸作成時の98%であった。
液体供給部40が2次側に接続された分離膜モジュール(図3参照)を、実施例1と同様に分離膜モジュールを図7に示す膜分離型連続発酵装置200Aに接続し、蒸気滅菌を行った。はじめに、発酵槽1に水を10L添加し、水を循環ポンプ8により発酵槽1および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け、濾過ポンプ11を運転して分離膜の2次側に水を濾過し、分離膜の2次側に水を封入した。濾過は0.1m/dayの濾過流束で、5分間行った。水の封入は、濾過バルブ13、洗浄液バルブ14、封入液体供給バルブ22、および排出バルブ27を閉止することにより行った。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側にある水を排出した。事前に水の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、水は85%封入されていた。
その後、液体供給バルブ22を開けて、液体供給部から、液体供給ポンプ21で、水を分離膜モジュール2へ供給し、分離膜モジュール2の2次側から1次側へ逆圧濾過し、分離膜の2次側に水を供給した。濾過は0.02m/dayの濾過流束で連続的に行った。
蒸気滅菌後、発酵槽1の温度が100℃まで低下した時点から、気体供給バルブ30を開として、圧縮空気を分離膜モジュール2内へ供給し、分離膜モジュール2が陰圧にならないようにして放冷した後、分離膜モジュール2の2次側に封入された水を排出して分離膜モジュール2の滅菌処理を終了した。
この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が10回まで参考例3のリークテストで問題はなかった。また蒸気滅菌処理を10回行なった後の中空糸膜モジュールの純水透水量は、中空糸作成時の99%であった。
実施例1と同様に分離膜モジュールを図5に示す膜分離型連続発酵装置200Aに接続し、蒸気滅菌を行った。
はじめに、発酵槽1に10Lの水を添加し、循環ポンプ8で、水を発酵槽1と分離膜モジュール2との間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して、分離膜モジュール2の2次側に水を濾過し、分離膜の2次側に水を封入した。濾過は0.1m/dayの濾過流束で、5分間行った。水の封入は、濾過バルブ13、洗浄液バルブ14、および排出バルブ27を閉止することにより行なった。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側にある水を排出した。事前に水の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、水は85%封入されていた。
次に、発酵槽1で水温を50℃まで昇温した。その後、濾過バルブ13、洗浄液バルブ14、排出バルブ27、液体供給バルブ22を閉止し、循環バルブ17を開とした。この状態で、循環ポンプ8を起動して、分離膜モジュール2に50℃温水をクロスフロー循環した。循環開始5分後、クロスフロー循環したまま、発酵槽の温度を1℃/分で80℃まで昇温した。その後、発酵槽1、分離膜モジュール2の1次側およびクロスフローの配管中の温水を系外に排出した。
実施例1と同様に分離膜モジュールを図7に示す膜分離型連続発酵装置200Aに接続し、蒸気滅菌を行った。はじめに、発酵槽1に水を1L添加し、水を循環ポンプ8で発酵槽1、および分離膜モジュール2間を循環させた。その後、分離膜モジュール2の濾過バルブ13を開け濾過ポンプ11を運転して濾過を行い、分離膜の2次側に水を封入した。その後、発酵槽1や循環ライン、分離膜モジュール2の1次側にある水を排出した。また、濾過バルブ13と排出バルブ27を開とし、分離膜の2次側中の水を排出した。事前に同条件で水の封入量を測定した結果、中空糸膜の濾過部分の2次側容積に対し、水は10%封入されていた。その後、蒸気供給部20により発酵槽1に125℃に制御された飽和水蒸気を供給し、発酵槽が121℃になった後、循環ライン、循環ポンプ8、分離膜モジュール2などに水蒸気を供給した。分離膜モジュール2の中空糸膜束の中心部に熱電対を設置し、分離膜モジュール2内の温度を観察した。熱電対の温度が123℃になるまで水蒸気を供給して分離膜モジュール2を昇温し、分離膜モジュール2を123℃以上で20分保持した後、水蒸気の供給を停止し、分離膜モジュール2の滅菌処理を終了した。滅菌処理を終了後、発酵槽1の温度が100℃まで低下した時点から、気体供給バルブ30を開として、圧縮空気を分離膜モジュール2内へ供給し、分離膜モジュール2が陰圧にならないようにして冷却した。分離膜モジュール2への水蒸気の供給は、合計55分であった。この分離膜モジュール2の蒸気滅菌を繰り返し、蒸気滅菌処理回数が4回目で参考例3のリークテストで不合格であった。また蒸気滅菌処理回数が3回目の中空糸膜モジュールの純水透水量は、中空糸作成時の90%であった。
2:分離膜モジュール
3:温度制御装置
4:撹拌装置
5:pHセンサー・制御装置
6:レベルセンサー・制御装置
7:差圧センサー・制御装置
8:循環ポンプ
9:原料供給ポンプ
10:中和剤供給ポンプ
11:濾過ポンプ
12:洗浄液供給ポンプ
13:濾過バルブ
14:洗浄液バルブ
15:気体供給装置
16:水供給ポンプ
17:循環バルブ
18:分離膜洗浄装置
19:供給バルブ
20:蒸気供給部
21:液体供給ポンプ
22:液体供給バルブ
23、25、34:配管
24:濾過液排出ライン
26、33:排出ライン
27、32:排出バルブ
29:洗浄液供給ライン
30:気体供給バルブ
31:液体供給ライン
40:液体供給部
50:制御装置
100、100A、100B:滅菌用装置
200、200A:膜分離型連続発酵装置
Claims (9)
- 分離膜モジュールを水蒸気により滅菌する方法であって、
前記分離膜モジュールの2次側に、分離膜のうちろ過に供される部分で囲まれた空間における充填率が70%以上となるように、大気圧下での沸点が80℃以上の液体を供給する液体供給工程と、
前記液体供給工程において前記分離膜モジュールの2次側に供給された液体の充填率が70%以上となるよう2次側を封止する液体封入工程と、
分離膜モジュールの2次側を封止したまま、前記分離膜モジュールの1次側に水蒸気を供給することで、前記分離膜モジュールを滅菌する滅菌工程と、
を含むことを特徴とする分離膜モジュールの滅菌方法。 - 前記液体供給工程は、前記滅菌工程の前に行われ、かつ、前記分離膜モジュールの1次側から2次側へ前記分離膜を介して前記液体を通液、または2次側に直接前記液体を供給するとともに、該2次側から1次側に前記分離膜を介して前記液体を通液することを含み、
前記滅菌方法は、前記液体封入工程後、前記滅菌工程の前に、前記分離膜モジュールの1次側の液体を排出する排出工程をさらに含むことを特徴とする請求項1に記載の分離膜モジュールの滅菌方法。 - 前記液体供給工程で前記分離膜モジュールに供給される液体は水であることを特徴とする
請求項1または2に記載の分離膜モジュールの滅菌方法。 - 前記滅菌工程後、前記分離膜モジュールの2次側に封入した液体を排出するとともに、該2次側に、前記供給工程で供給された液体と同種類または異なる液体を供給して分離膜モジュールを冷却する冷却工程をさらに含むことを特徴とする請求項1~3のいずれかに記載の分離膜モジュールの滅菌方法。
- 前記滅菌工程後、前記分離膜モジュールの2次側に封入した液体を排出する排出工程と、
前記分離膜モジュールの2次側に洗浄液を供給し、かつ前記分離膜モジュールの2次側から前記洗浄液を排出することで、該2次側内部を濯ぐとともに、前記分離膜モジュールを冷却する冷却工程と、
をさらに含むことを特徴とする請求項1~4のいずれかに記載の分離膜モジュールの滅菌方法。 - 前記昇温工程前に、前記分離膜モジュール内に温水を供給して予熱する予熱工程をさらに含むことを特徴とする請求項1~5のいずれかに記載の分離膜モジュールの滅菌方法。
- 前記滅菌工程は、前記分離膜の2次側から1次側へ液体を通液しながら、前記分離膜の1次側へ水蒸気を供給することを含む請求項1~6のいずれかに記載の分離膜モジュールの滅菌方法。
- 請求項1~7のいずれかに記載の滅菌方法により前記分離膜モジュールを滅菌する蒸気滅菌工程と、
発酵原料を微生物の発酵培養により化学品を含有する発酵液へと変換する発酵工程と、
前記蒸気滅菌工程後の前記分離膜モジュールにより前記発酵液から濾過液として化学品を回収する膜分離工程と、
を含むことを特徴とする連続発酵による化学品の製造方法。 - 発酵原料を微生物によって発酵培養することにより、該発酵原料を、化学品を含有する発酵液に変換する発酵槽と、
前記発酵液から化学品を分離する分離膜モジュールと、
前記発酵槽から前記分離膜モジュールに発酵液を送液する発酵液循環部と、
前記発酵槽および前記分離膜モジュールに水蒸気を供給する蒸気供給部と、
前記分離膜モジュールの2次側に大気圧下での沸点が80℃以上の液体を供給する液体供給部と、
前記蒸気供給手段の稼働中に、前記分離膜モジュールの2次側であって、分離膜のうちろ過に供される部分で囲まれた空間における前記液体の充填率が70%以上となるように、前記分離膜モジュールの2次側を封止する封止部と、を備えることを特徴とする膜分離型連続発酵装置。
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/385,627 US20150050694A1 (en) | 2012-03-16 | 2013-03-01 | Method of sterilizing separation membrane module, method of producing chemical by continuous fermentation, and membrane separation-type continuous fermentation apparatus |
JP2013516876A JP6015654B2 (ja) | 2012-03-16 | 2013-03-01 | 分離膜モジュールの滅菌方法、連続発酵による化学品の製造方法、および膜分離型連続発酵装置 |
BR112014022787-0A BR112014022787B1 (pt) | 2012-03-16 | 2013-03-01 | método para esterilizar um módulo de membrana de separação |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012-060694 | 2012-03-16 | ||
JP2012060694 | 2012-03-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2013137027A1 true WO2013137027A1 (ja) | 2013-09-19 |
Family
ID=49160941
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2013/055711 WO2013137027A1 (ja) | 2012-03-16 | 2013-03-01 | 分離膜モジュールの滅菌方法、連続発酵による化学品の製造方法、および膜分離型連続発酵装置 |
Country Status (4)
Country | Link |
---|---|
US (1) | US20150050694A1 (ja) |
JP (1) | JP6015654B2 (ja) |
BR (1) | BR112014022787B1 (ja) |
WO (1) | WO2013137027A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7022247B1 (ja) | 2021-02-01 | 2022-02-17 | 岩井ファルマテック株式会社 | 精製水供給システム |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017069219A1 (ja) * | 2015-10-20 | 2017-04-27 | 三菱レイヨン株式会社 | 膜の殺菌方法および該殺菌方法を実施し得るガス溶解液製造装置 |
US20210260533A1 (en) * | 2019-02-14 | 2021-08-26 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Natural Resources | Method for fouling reduction on the surface of ceramic membranes using steam fast-flushing |
KR102335124B1 (ko) * | 2021-06-25 | 2021-12-03 | 안태완 | 농산부산물 발효 사료 제조장비 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5925750A (ja) * | 1982-07-31 | 1984-02-09 | カゴメ株式会社 | 逆浸透装置におけるモジユ−ルの殺菌方法 |
JPS61242604A (ja) * | 1985-04-18 | 1986-10-28 | Asahi Chem Ind Co Ltd | 濾過装置の蒸気滅菌方法 |
JPS6253655A (ja) * | 1985-09-03 | 1987-03-09 | ダイセル化学工業株式会社 | 半透膜モジユ−ルの蒸気滅菌方法 |
JPH02207826A (ja) * | 1989-02-07 | 1990-08-17 | Daicel Chem Ind Ltd | 蒸気滅菌方法 |
JPH08164328A (ja) * | 1994-12-12 | 1996-06-25 | Nitto Denko Corp | 濾過装置の蒸気滅菌後の冷却方法 |
JPH09220445A (ja) * | 1996-02-15 | 1997-08-26 | Kanegafuchi Chem Ind Co Ltd | 中空糸膜モジュールの逆洗滅菌方法 |
JP2008237213A (ja) * | 2007-02-26 | 2008-10-09 | Toray Ind Inc | 連続発酵装置 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5503750A (en) * | 1993-10-04 | 1996-04-02 | Russo, Jr.; Lawrence J. | Membrane-based process for the recovery of lactic acid by fermentation of carbohydrate substrates containing sugars |
DE59707888D1 (de) * | 1996-12-21 | 2002-09-05 | Mat Adsorption Technologies Gm | Membranmodul mit schichtförmig angeordneten hohlfasermembranen |
KR20120095959A (ko) * | 2009-11-10 | 2012-08-29 | 도레이 카부시키가이샤 | 화학품 제조용 중공사막 모듈 및 화학품의 제조 방법 |
BR112013016065A2 (pt) * | 2010-12-24 | 2016-09-20 | Toray Industries | método de esterilização de um módulo de membrana de separação, dispositivo de esterilização e aparelho de produção de produtos químicos |
JP2013128470A (ja) * | 2011-12-22 | 2013-07-04 | Toray Ind Inc | 分離膜モジュールの滅菌方法、化学品の製造方法、分離膜モジュールの滅菌用装置および膜分離型連続発酵装置 |
-
2013
- 2013-03-01 WO PCT/JP2013/055711 patent/WO2013137027A1/ja active Application Filing
- 2013-03-01 BR BR112014022787-0A patent/BR112014022787B1/pt not_active IP Right Cessation
- 2013-03-01 US US14/385,627 patent/US20150050694A1/en not_active Abandoned
- 2013-03-01 JP JP2013516876A patent/JP6015654B2/ja active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5925750A (ja) * | 1982-07-31 | 1984-02-09 | カゴメ株式会社 | 逆浸透装置におけるモジユ−ルの殺菌方法 |
JPS61242604A (ja) * | 1985-04-18 | 1986-10-28 | Asahi Chem Ind Co Ltd | 濾過装置の蒸気滅菌方法 |
JPS6253655A (ja) * | 1985-09-03 | 1987-03-09 | ダイセル化学工業株式会社 | 半透膜モジユ−ルの蒸気滅菌方法 |
JPH02207826A (ja) * | 1989-02-07 | 1990-08-17 | Daicel Chem Ind Ltd | 蒸気滅菌方法 |
JPH08164328A (ja) * | 1994-12-12 | 1996-06-25 | Nitto Denko Corp | 濾過装置の蒸気滅菌後の冷却方法 |
JPH09220445A (ja) * | 1996-02-15 | 1997-08-26 | Kanegafuchi Chem Ind Co Ltd | 中空糸膜モジュールの逆洗滅菌方法 |
JP2008237213A (ja) * | 2007-02-26 | 2008-10-09 | Toray Ind Inc | 連続発酵装置 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7022247B1 (ja) | 2021-02-01 | 2022-02-17 | 岩井ファルマテック株式会社 | 精製水供給システム |
JP2022117924A (ja) * | 2021-02-01 | 2022-08-12 | 岩井ファルマテック株式会社 | 精製水供給システム |
Also Published As
Publication number | Publication date |
---|---|
JP6015654B2 (ja) | 2016-10-26 |
JPWO2013137027A1 (ja) | 2015-08-03 |
US20150050694A1 (en) | 2015-02-19 |
BR112014022787B1 (pt) | 2021-04-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP2500083A1 (en) | Hollow fiber membrane module for use in production of chemical substance, and process for production of chemical substance | |
WO2012090556A1 (ja) | 連続発酵による化学品の製造方法 | |
EP3012012B1 (en) | Filtration device, use of such filtration device, and method for operating such filtration device | |
JP6015654B2 (ja) | 分離膜モジュールの滅菌方法、連続発酵による化学品の製造方法、および膜分離型連続発酵装置 | |
US20130280773A1 (en) | Method for producing chemical by continuous fermentation | |
WO2012086763A1 (ja) | 分離膜モジュールの滅菌方法、滅菌用装置および化学品製造用装置 | |
JP2013226486A (ja) | 分離膜モジュールの滅菌方法、化学品の製造方法および膜分離型連続発酵装置 | |
JP2011188791A (ja) | 連続発酵装置の運転方法 | |
JP2012161288A (ja) | 化学品の製造装置および化学品の製造方法 | |
JP2013128470A (ja) | 分離膜モジュールの滅菌方法、化学品の製造方法、分離膜モジュールの滅菌用装置および膜分離型連続発酵装置 | |
JP2012179018A (ja) | 化学品の製造装置および化学品の製造方法 | |
EP2617832B1 (en) | Production method for chemicals by continuous fermentation | |
JP2008099667A (ja) | アルコール製造方法及び製造装置 | |
JP2010161987A (ja) | 発酵によるブタノールの製造方法 | |
JP2013188149A (ja) | 分離膜モジュールの蒸気滅菌方法、連続発酵による化学品の製造方法および膜分離型連続発酵装置 | |
JP2013188150A (ja) | 分離膜モジュールの蒸気滅菌方法、連続発酵による化学品の製造方法、分離膜モジュールの滅菌用装置および膜分離型連続発酵装置 | |
JP2011193787A (ja) | 連続発酵装置の運転方法 | |
JP2012179019A (ja) | 連続発酵による化学品の製造方法 | |
CN110291201A (zh) | 采用连续发酵的醇的制造方法及该制造方法所使用的连续发酵装置 | |
JP2012210184A (ja) | 連続発酵による化学品の製造方法 | |
JP2012135249A (ja) | 連続発酵による化学品の製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
ENP | Entry into the national phase |
Ref document number: 2013516876 Country of ref document: JP Kind code of ref document: A |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 13760832 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: IDP00201405505 Country of ref document: ID Ref document number: 14385627 Country of ref document: US |
|
REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112014022787 Country of ref document: BR |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 13760832 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 112014022787 Country of ref document: BR Kind code of ref document: A2 Effective date: 20140915 |