Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/14—Vegetable proteins
  • A23J3/18—Vegetable proteins from wheat
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/22—Working-up of proteins for foodstuffs by texturising
  • A23J3/225—Texturised simulated foods with high protein content
  • A23J3/227—Meat-like textured foods
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
  • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
  • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/14—Vegetable proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
  • A23L33/17—Amino acids, peptides or proteins
  • A23L33/185—Vegetable proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
  • A23L7/10—Cereal-derived products
  • A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/14—Fungi; Culture media therefor

Definitions

• the invention relates to a vegetable-based food product having the texture of minced meat and a method for its preparation.
• the invention relates to a vegetable- based food product comprising proteinaceous material derived from gluten.
• Beans of different varieties are a well-known source of protein. Beans are commonly prepared by soaking in water overnight and then cooking. It is also well-known to treat the cooked beans with a fungus which enables this highly proteinaceous material to be formed into a cake. This process is known to many as the tempeh process. The tempeh process increases digestibility of the raw material by 5 to 10 %.
• Tempeh also spelled tempe is an Indonesian foodstuff based on beans (normally soybeans), which has a tradition dating back many centuries in Java. Its popularity is increasing in various regions including Japan, the USA, and Western Europe. It is made by a natural fermentation process involving a Rhizopus fungus plus many different bacteria and yeasts. The process binds the beans into a cake form, similar to a firm vegetarian burger patty.
• tofu which is derived from soybeans
• tempeh is a whole bean product having different nutritional characteristics and textural qualities. The tempeh fermentation process, and its retention of the whole bean, means that the product has a higher content of protein, dietary fibre, and vitamins.
• the fermentation process improves some characteristics of soybeans including protein level (up to 40 % of the dry mass), fatty acid composition (Hering, L, et a/., Lipid / Fett, 1991 , 93(8), 303-308), the level and pattern of oligosaccharides (Rehms, H. and Barz, W., Appl. Microbiol. Biotechnol., 1995, 44, 47-52), and the amount of several vitamins, especially vitamin B12 and vitamin D (Keuth, S. and Bisping, B., J. Appl. Bacterid., 1993, 75, 427-434; Denter, J., et a/., J.
• the product is normally consumed in the form of slices that have been fried, as a type of Indonesian satay, as a peppered paste (sambal), or as vegetarian tempeh burger.
• soybeans as a source of proteins for preparing meat alternative (or replacer) products using a tempeh process relate to both texture and taste.
• the use of whole soybeans gives neither a texture nor a taste similar to minced meat.
• the whole beans remain visible in the product, can be felt in the mouth when chewing, and also give a nutty soya flavour. While digestibility is increased compared to regular cooking of beans, a large portion of the soya bean still remains indigestible by the human body.
• gluten especially wheat gluten
• wheat gluten as an affordable protein source
• the object of the present invention is therefore to provide a vegetarian minced meat replacer which has similar taste and texture attributes compared to minced meat or at least to provide a useful alternative to existing meat replacers.
• the invention relates to a vegetable-based food product comprising at least 50 wt% (% of dry weight) protein, wherein the protein is gluten or is proteinaceous material derived from gluten, and wherein the product has the texture of minced meat.
• the gluten is preferably wheat gluten, barley gluten, rice gluten or rye gluten.
• the food product also comprises any one or more of starch, flour, and bran.
• the food product further comprises added meat flavour, for example a vegetable-based meat flavour.
• a second aspect of the invention there is provided a method for preparing a vegetable-based food product comprising: a) contacting a gluten containing plant material in solid form with water for 12 to 48 hours at a pH less than 5;
• the plant material comprises gluten in the amount of at least 50 % by weight, preferably at least 70 % by weight, more preferably at least 75 % by weight.
• the gluten containing plant material is preferably in the form of pellets, for example extruded pellets.
• the pellets may comprise flour and/or bran in addition to gluten.
• the plant material comprises gluten in the amount of at least 70 % by weight, more preferably at least 75 % by weight, even more preferably at least 80 % by weight.
• the gluten containing plant material is preferably obtained from wheat.
• the fermented product of step c) is normally in the form of a solid or semi-solid cake.
• the cake is texturised to give a food product having a texture of minced meat.
• the contact time in step a) is preferably 16 to 24 hours.
• the pH is reduced to or maintained at less than 5 by addition of an organic acid, for example lactic acid, citric acid, acetic acid or malic acid.
• an organic acid for example lactic acid, citric acid, acetic acid or malic acid.
• the heating temperature of step b) is preferably in the range 100 to 130 °C, for example 120 °C, and the heating time is 3 to 10 minutes, preferably 5 minutes.
• the mould is selected from any one of the species
• Rhizopus Rhizopus, Mucor, Neurospora, and Amylomyxes.
• a meat flavour ingredient may be incorporated into the plant material before step a) or during step a).
• the invention relates to a vegetable-based food product comprising at least 50 % by weight protein, wherein the protein is gluten or is proteinaceous material derived from gluten, and wherein the product has the texture of minced meat.
• the invention also relates to a method for preparing a vegetable-based food product including a step of fermenting a gluten- based material with a mould and then processing the fermented product into a vegetable- based food product for use as a replacement for minced meat.
• gluten refers to the protein fraction of wheat, barley, rice, rye, or a combination thereof, after starch is removed by extraction.
• vegetable-based means any material that is based on, or derived from, vegetable matter of any kind.
• proteinaceous material derived from gluten means a protein mixture or extract comprising at least 80 % of a gluten fraction obtained from wheat, barley, rice, rye or a combination thereof after starch extraction. The remaining up to 20 % may be starch residues, and fatty acids.
• meal flavour means any vegetable-based flavour that mimics the flavour of any meant.
• the gluten containing plant material used as the raw material in the process for preparing the food product of the invention should be in solid form, ideally in the form of solid pellets, granules, or similar particles.
• the reason for this is that gluten is sparingly soluble in water and will usually form a lump.
• the mould will form biomass separate from the gluten lump.
• the gluten lump will slowly dissolve due to enzymes secreted from the mould.
• Wetted gluten powder does not work because the mould needs space to grow the mycelium.
• the spaces that exist between larger particles, e.g. pellets, provide for basic oxygen transfer, which is important for mycelium growth.
• the gluten containing plant material is porous, i.e. the plant material which may be in the form of pellets, granules or other similar particles, has a multitude of empty spaces or voids within the solid material matter of such particles.
• the plant material is first soaked in water for 12 to 48 hours in order to soften the dried particles.
• the water incorporated into the particles will allow the mould to form the mycelium.
• the pH of this step must be lower that 5 to prevent microbial growth. At higher pH, an unacceptably high amount of spores of spoilage bacteria can grow during the soaking step and potentially produce heat stable toxins. Bacterial growth may also be prevented by adding salt or alcohol, but then the mould will most likely not grow.
• the wetted solid plant material (normally wetted pellets) is then sterilised to deactivate any microbes present by heating to at least 100 °C for a few minutes. Sterilisation is important for effective incubation of the plant material with the mould.
• the fermented product obtained following incubation with the mould is in the form of a solid or semi-solid lump or cake.
• the mycelium is able to penetrate the particles of solid plant material due to the rough/porous surface of the particles. This leads to mycelium formation in the spaces between particles and binds the particles together to form a solid or semi-solid lump or cake.
• the mould used may be any mould that is able to grow on the plant material and that has a safe history in food (i.e. no toxin production), and includes a mould of any one of the species Rhizopus, Mucor, Neurospora, and Amylomyxes. It is known that some strains of Rhizopus can produce toxins and so these strains are not suitable for this invention.
• the cake formed in the process is sufficiently moist that it can be manipulated by stirring or mixing, or some other kind of mechanical agitation, in a controlled manner to provide a product have the texture of minced meat.
• the texture may be described as comprising small soft lumps often in combination with soft stringy material, and is what would normally be considered as the texture of meat that has been minced using well-known techniques.
• the product may be further processed into different forms, for example by pre- cooking (e.g. frying), d rying, or incorporating into a sauce, such as a pasty sauce (e.g. Bolognese sauce).
• pre- cooking e.g. frying
• d rying e.g. d rying
• a sauce such as a pasty sauce (e.g. Bolognese sauce).
• Meat flavour ingredients may be incorporated into the product at any stage of the preparation process. Such ingredients would usually be vegetable-based flavour ingredients to preserve the integrity of the product as a vegetarian food.
• the flavour ingredient can be incorporated within the plant material before extrusion into pellets for example. Alternatively, the flavour ingredient can be added to the solid plant material during the soaking stage, i.e. during step a) rather than beforehand. The flavour ingredient may also be incorporated during later processing stages.
• the process of the invention has the benefit that vitamins not present in the raw material (such as vitamins A, E, B3, B6, K etc.) may be provided in the product, or the amount of certain vitamins already present are increased.
• the process of the invention may beneficially alter the amino acid composition by increasing the levels of essential amino acids.
• Example 1 describes a standard process for obtaining the product using any gluten-based pelletised raw material and the process parameter ranges used for solid state fermentation.
• Examples 2 and 3 describe two ways to incorporate a vegetable-based meat flavour into the product at different process steps, either during the soaking step (Example 2) or during the extrusion step used for making the gluten pellets (Example 3).
• Example 4 shows a third way, i.e. after the downstream processing using a vacuum sealing machine.
• Example 5 describes the preparation of a dehydrated product and its rehydration.
• Example 1 General method for preparing minced meat alternative product
• Porous extruded gluten pellets comprising a plant protein fraction of 76.7 wt%, flour and/or bran were soaked for 16 h at room temperature (23 °C) in water adjusted to a pH between 4 and 5 using an organic acid (e.g. acetic acid, citric acid or lactic acid).
• the raw material and the water were mixed in a ratio so that no water remained after soaking.
• the wet raw material was cooked at high temperature (e.g. 121 °C for 10 min). After cooling, the raw material was inoculated with a spore suspension of a mould from the genus Rhizopus, Mucor, Neurospora or Amylomyces.
• the spore load per gram of wet material is in the range of 10 4 to 10 6 cfu/g wet material.
• the raw material was homogeneously mixed with the spore suspension, and the inoculated material incubated at 30 to 37 °C for 24 to 48 h at a relative humidity of 70 to 90 %. After the fermentation, the raw material was bound together to form a firm cake due to vigorous mould mycelium growth. The cake was carefully agitated or mixed (e.g. stirred at low speed) to generate an inhomogeneous minced meat structure. The material was then cooked at 100 °C for 8 min to inactivate the mould and any secreted enzymes to ensure shelf stability. The cooked material was stored at -20 °C to +4 °C until further use.
• Example 2 Incorporation of meat flavour into soaking step
• Extruded gluten pellets were prepared according to the general method of Example 1 .
• a vegetable-based meat flavour was added in a concentration of 1 -10 % (w/v).
• Downstream processing was also conducted according to Example 1 .
• the product obtained had essentially the same texture and appearance, and a distinct meat flavour taste.
• Example 3 Incorporation of meat flavour before soaking step
• Example 1 The general method of Example 1 was followed, except that the gluten pellets were extruded incorporating a vegetable-based meat flavour in a concentration of 1 -10 % (w/w). Downstream processing was also conducted according to Example 1 . The product obtained had essentially the same texture and appearance, and a distinct meat flavour taste.
• Example 2 The general method of Example 1 was followed. The resulting material was carefully pressed to remove most of the water, without destroying its structure. A liquid vegetable- based meat flavour preparation was added at a ratio of 1 to 10 %. The preparation was put into plastic bags and subsequently into a vacuum sealing machine. This sealed mixture was stored at -20 °C.
• Example 1 The general method of Example 1 was followed. The resulting material was vacuum dried at 60 °C for 16 h at 10 mbar. The dried material was rehydrated prior to use at 100 °C for 10 min using water containing 1 -10 % (w/v) sodium chloride.

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• General Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Meat, Egg Or Seafood Products (AREA)
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• 2012
  • 2012-12-10 DK DK12798302.1T patent/DK2790525T3/en active
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