NZ625536B2 - Vegetable-based minced meat alternative - Google Patents

Abstract

Provided is a method for preparing a vegetable-based food product including the step of fermenting plant material containing at least 50% by weight gluten using a microorganism from the Rhizopus, Mucor, Neurospora or Amylomyxe genera. The food product prepared can be used as a replacement for minced meat. meat.

Description

PCT/EP2012I074913 VEGETABLBBASED MINCED MEAT ALTERNATIVE TECHNICAL FIELD The invention relates to a vegetable-based food product having the texture of minced meat and a method for its preparation. in particular, the invention relates to a vegetable- based food product comprising proteinaceous material derived fr0m gluten.

OUND The rising world population is placing increasing demands on the supply of foods, especially those that are a source of protein. The production and consumption of meat as a protein source is also becoming increasingly unsustainable. The high price of meat means that its availability in many parts of the world is limited, if not non-existent in some areas.

(,A~"”‘":\ Most of the s less expensive n sources originate from a variety of pulses, grains and lentils. These sources usually have a high concentration of protein, but also have some drawbacks in t of digestibility and anti-nutritional factors. Moreover, for many people. these cheaper protein sources do not have a very pleasant taste or texture compared with meat products.

There has been an ongoing search for solutions to the above problems by ing affordable high protein alternatives to meat products, especially for developing or emerging market countries.

Beans of different varieties are a well—known source of protein. Beans are commonly prepared by soaking in water overnight and then cooking. it is also nown to treat the cooked beans with a fungus which enables this highly proteinaceous al to be formed into a cake. This process is known to many as the tempeh s. The tempeh process ses digestibility of the raw material by 5 to 10 %.

Tempeh (also spelled tempe) is an Indonesian foodstuff based on beans (normally soybeans), which has a tradition dating back many centuries in Java. lts popularity is increasing in various regions including Japan, the USA, and Western Europe. it is made by a natural fermentation s involving a us fungus plus many different ia and yeasts. The process binds the beans into a cake form, similar to a firm vegetarian burger patty. in contrast to tofu, which is derived from ns, tempeh is a whole bean product having different nutritional characteristics and textural qualities. The tempeh fermentation process, and its retention of the whole bean, means that the product has a higher content of protein, dietary fibre, and vitamins. in particular, the fermentation process improves some characteristics of soybeans including protein level (up to 40 % of the dry mass), fatty acid composition (Hering, L., et a/., Lipid / Fett, 1991, 93(8), 303-308), the level and n of oligosaccharides (Rehms, H. and Barz, W., Appl. Microbiol. Biotechnoi., 1995, 44, 47-52), and the amount of l vitamins, especially vitamin 812 and vitamin D (Keuth, S. and Bisping, B., J. Appl. Bacteriol., 1993, 75, 427-434; Denter, J., et al., J. Food Mycol., $998, 1, 149461). The product is normally consumed in the form of slices that have been fried, as a type of indonesian satay, as a peppered paste l), or as vegetarian tempeh burger.

The problems with using soybeans as a source of proteins for preparing meat alternative (or replacer) products using a tempeh process relate to both texture and taste. The use of whole soybeans gives neither a texture nor a taste similar to minced meat. The whole beans remain visible in the product. can be felt in the mouth when chewing, and also give a nutty soya flavour. While digestibility is increased compared to regular cooking of beans, 8 large portion of the soya bean still remains indigestible by the human body. in the search for new sources of protein that may be subjected to a fermentation process similar to the tempeh process, the focus has been on n sources, such as beans, that can provide a rich source of nutrients to a fungus. Thus, most beans are a good source of carbohydrates, fatty acids, ns and minerals, as well as n. Sources that are high in n, but limited in other materials, have previously been considered to be unsuitable for supporting the growth of a fungus in a tempeh fermentation. Gluten from sources such as wheat, barley, rice, and rye contain protein and small amounts of starch. but not much more.

Gluten has therefore not previously been considered as a protein source in the tempeh process.

However, the applicant has now found that gluten, especially wheat gluten, as an affordabie n , can be processed using the pies of tempeh fermentation to e a new meat alternative.

Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field.

It is an object of the present invention to overcome or rate at least one of the disadvantages of the prior art, or to provide a useful alternative.

An object of a red embodiment of the present invention is therefore to provide a rian minced meat replacer which has similar taste and texture attributes compared to minced meat or at least to provide a useful alternative to existing meat replaoers.

STATEMENTS OF THE iNVENTlON In a first aspect, the invention relates to a method for preparing a vegetable-based food product comprising: a) contacting a gluten containing plant material in solid form with water for 12 to 48 hours at a pH less than 5; b) heating the plant material at a temperature and for a time sufficient to sterilise the plant material; c) cooling and then mixing the plant material and incubating to give a fermented product wherein a microorganism selected from Rhizopus, Mucor, Neurospora or Amylomyxe is used for fermentation; and d) sing the fermented product into the vegetable-based food product; wherein the plant material comprises gluten in the amount of at least 50 % by weight.

In a second aspect, the ion relates to a product obtained by the process of the first aspect. in a third aspect, the invention relates to the use of the food product of the second aspect as a vegetable~based replacement for minced meat.

Also described is a vegetabie-based food product comprising at least 50 wt% protein, wherein the protein is gluten or is proteinaceous al derived from , and has been fermented by a mould selected from the species selected from the group ting of Rhizopus, Mucor, Neurospora and Amylomyxes, and wherein the t has the texture of minced meat. The gluten is ably wheat, barley gluten, rice gluten or rye gluten.

Preferably, the food product also comprises any one or more of starch, flour, and bran. in preferred embodiments of the invention the food product further comprises added meat flavour, for example a ble-based meat flavour.

The gluten containing plant al is preferably in the form of pellets, for example extruded pellets. The pellets may se flour and/or bran in addition to gluten.

Preferably. the plant material comprises gluten in the amount of at least 70 % by weight, more preferably at least 75 % by weight. even more preferably at least 80 % by weight.

The gluten containing plant material is ably obtained from wheat.

The ted product of step c) is normally in the form of a solid or semi—solid cake.

In preferred embodiments, the cake is texturised to give a food product having a texture of minced meat.

The contact time in step a) is preferably 16 to 24 hours.

Preferably, the pH is reduced to or maintained at less than 5 by addition of an organic acid, for example lactic acid, citric acid, acetic acid or malic acid.

The heating temperature of step b) is preferably in the range 100 to 130 °C, for example 120 °C, and the heating time is 3 to 10 minutes, preferably 5 minutes. in preferred embodiments, the mould is selected from any one of the species Rhizopus, Mucor, Neurospora, and Amy/omyxes.

A meat flavour ingredient may be incorporated into the plant material before step a) or during step a).

Unless the context y requires otherwise, throughout the description and the claims, the words “comprise”, “comprising”, and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of “including, but not limited to”.

DETAILED PTION The invention relates to a vegetable—based food product comprising at least 50 % by weight protein, wherein the protein is gluten or is proteinaceous material derived from gluten, and n the product has the texture of minced meat. The ion also relates to a method for preparing a vegetable~based food product including a step of fermenting a giuten~ [wam based material with a mould and then processing the fermented t into a vegetable- based food t for use as a replacement for minced meat.

The term "gluten" refers to the protein fraction of wheat, barley, rice, rye, or a combination thereof, after starch is d by extraction.

The term "vegetable-based” means any material that is based on, or derived from, vegetable matter of any kind.

The term “proteinaceous material derived from gluten” means a protein mixture or extract comprising at least 80 % of a gluten fraction obtained from wheat, barley, rice, rye or a combination thereof after starch extraction. The remaining up to 20 % may be starch residues, and fatty acids.

The term “meat flavour” means any vegetable-based flavour that mimics the flavour of any meant.

The gluten containing plant material used as the raw material in the process for preparing the food product of the invention should be in solid form, ideally in the form of solid pellets, granules, or similar particles. The reason for this is that gluten is sparingly e in water and will usually form a lump. in a ged fermentation system (excess of water) using gluten powder, the mould will form biomass separate from the gluten lump. The gluten lump will slowly dissolve due to enzymes secreted from the mould. Wetted gluten powder does not work because the mould needs space to grow the mycelium. The spaces that exist between larger particles, e.g. pellets, e for basic oxygen transfer, which is important for mycelium growth.

Preferably, the gluten containing plant material is porous, i.e. the plant al which may be in the form of pellets, granules or other similar les, has a ude of empty spaces or voids within the solid material matter of such les. . 25 The plant material is first soaked in water for 12 to 48 hours in order to soften the dried particles. The water incorporated into the particles will allow the mould to form the mycelium.

The pH of this step must be lower that 5 to t microbial growth. At higher pH. an ptably high amount of spores of spoilage bacteria can grow during the soaking step and potentially produce heat stable toxins. Bacterial growth may also be prevented by adding salt or alcohol, but then the mould will most likely not grow.

The wetted solid plant material (normally wetted s) is then sterilised to deactivate any microbes present by heating to at least 100 °C for a few minutes. Sterilisation is important for effective incubation of the plant material with the mould.

The fermented product obtained following incubation with the mould is in the form of a solid or semi-solid lump or cake. The mycelium is able to penetrate the particles of solid plant material due to the rough/porous surface of the particles. This leads to mycelium 5 I3 formation in the spaces between particles and binds the les together to form a solid or olid lump or cake.

The mould used may be any mould that is able to grow on the plant material and that has a safe history in food (i.e. no toxin production), and es a mould of any one of the species Rhizopus, Mucor, pora, and Amylomyxes. it is knOWn that some strains of Rhizopus can produce toxins and so these strains are not suitable for this invention.

The cake formed in the process is sufficiently moist that it can be manipulated by stirring or mixing, or some other kind of mechanical ion, in a controlled manner to provide a product have the texture of minced meat. The texture may be bed as comprising small soft lumps often in c0mbination with soft stringy material, and is what would ly be considered as the texture of meat that has been minced using well-known techniques.

The product may be further processed into different forms, for example by pre- cooking (e.g. frying), drying, or orating into a sauce, such as a pasty sauce (e.g.

Bolognese sauce).

Meat flavour ingredients may be incorporated into the product at any stage of the preparation process. Such ingredients would usually be vegetable-based flavour ingredients to preserve the integrity of the product as a vegetarian food. The flavour ingredient can be orated within the plant material before extrusion into s for example. Alternatively, the flavour ingredient can be added to the solid plant material during the soaking stage, Le. during step a) rather than beforehand. The flavour ingredient may also be incorporated during later processing stages. in addition to the advantages of the invention bed above, the process of the invention has the benefit that vitamins not present in the raw material (such as vitamins A, E, BB, 86, K etc.) may be ed in the product, or the amount of certain vitamins already I .. present are increased. In addition, the process of the ion may beneficially alter the amino acid composition by increasing the levels of essential amino acids. in the following examples the general method for preparing a vegetarian mince meat replacer based on tempeh fermentation principles is describe in more detail. Example 1 describes a standard process for obtaining the product using any gluten-based pelletised raw material and the process parameter ranges used for solid state fermentation. Examples 2 and 3 describe two ways to orate a vegetable-based meat flavour into the prod uot at different process steps, either during the soaking step (Example 2) or during the extrusion step used for making the gluten pellets (Example 3). e 4 shows a third way, Le. after the downstream processing using a vacuum sealing machine. Example 5 describes the preparation of a dehydrated product and its rehydration.

W0 2013/087558 6 EXAMPLES The invention is further described with reference to the ing examples. It will be appreciated that the invention as claimed is not ed to be limited in any way by these examples.

Example 1: General method for preparing minced meat alternative product Porous extruded gluten peliets comprising a plant protein on of 76.7 wt%, flour and/or bran were soaked for 16 h at room temperature (23 °C) in water adjusted to a pH between 4 and 5 using an organic acid (eg. acetic acid, citric acid or lactic acid). The raw material and the water were mixed in a ratio so that no water remained after g. The wet raw al was cooked at high temperature (e.g. 121 °C for 10 min). After cooling, the raw material was inoculated with a spore suspension of a mould from the genus Rhizopus, Mucor, Neurospora or Amy/omyces. Preferably the spore load per gram of wet material is in i5 the range of 104 to 106 cfu/g wet material. The raw materiai was homogeneously mixed with the spore suspension, and the inoculated material incubated at 30 to 37 °C for 24 to 48 h at a relative humidity of 70 to 90 %. After the fermentation, the raw al was bound together to form a firm cake due to vigorous mouid mycelium growth. The cake was carefuliy agitated or mixed (e.g. stirred at iow speed) to generate an inhomogeneous minced meat structure.

The material was then cooked at 100 ”C for 8 min to inactivate the mould and any secreted enzymes to ensure shelf stability. The cooked material was stored at -20 °C to +4 °C until further use.

Table 1 wheat gluten R. o/rgosporus 2 5 x 10 - wheargiuten m105 - wheatgiuten 25x 106 24 wheatgiuten 2.5 x 106 24 iuten_ 6 25x10 mm 24 wheatgiuten- 6 25x10 24 wheat gluten 2.5x 105 "I. 24 WO 87558 7 e 2: Incorporation ofmeat flavour into soaking step ed gluten s were prepared according to the general method of Example 1.

In the soaking step, a vegetable-based meat flavour was added in a concentration of 1-10 % (w/v). Downstream processing was also ted according to Example 1. The product obtained had essentiaiiy the same texture and appearance, and a distinct meat flavour taste.

Example 3: Incorporation ofmeat flavour before soaking step The general method of Example 1 was followed, except that the gluten pellets were 1O extruded incorporating a vegetable-based meat fiavour in a concentration of 1-10 % (w/w).

Downstream processing was also conducted according to Example 1. The product obtained had essentially the same texture and appearance, and a distinct meatflavour taste.

Example 4: Incorporation ofmeat flavour in downstream processing The general method of Example 1 was followed. The resuiting materiai was carefully d to remove most of the water, without destroying its structure. A liquid vegetable- based meat r preparation was added at a ratio of 1 to 10 °/o. The preparation was put into plastic bags and subsequently into a vacuum sealing machine. This sealed mixture was stored at ~20 °C.

Example 5: Dehydration and rehydration The general method of Example 1 was followed. The resulting material was vacuum dried at 60 °C for 16 h at 10 mbar. The dried material was rehydrated prior to use at 100 °C for 10 min using water containing 1-10 % (w/v) sodium chloride. it is to be appreciated that although the invention has been described with reference to specific ments, variations and modifications may be made without departing from the scope of the ion as defined in the claims. Furthermore, where known equivalents exist to specific features, such equivalents are incorporated as if specificaily ed to in this specification.

Claims (19)

1. A method for preparing a vegetable~based food product sing: a) contacting a gluten containing plant material in solid form with water for 12 to 48 hours at a pH less than 5; b) heating the piant material at a temperature and for a time sufficient to ise the plant material; c) cooling and then mixing the plant material and incubating to give a fermented product wherein a microorganism selected from Rhizopus, Mucor. Neurospora or Amylomyxe is used for tation; and d) processing the fermented product into the vegetable—based food product; wherein the plant material comprises gluten in the amount of at least 50 % by weight.

2. A method for preparing a vegetable-based food product as claimed in claim 1 wherein gluten is present in an amount of at least 70 % by weight.

3. A method for preparing a vegetable—based food product as claimed in claim 1 wherein gluten is present in an amount of at least 75 % by weight.

4. A method as claimed in any one of claims 1 to 3, n the gluten ning plant material is in the form of pellets.

5. A method as d in claim 4, wherein the gluten is in the form of extruded pellets.

6. A method as claimed in claim 4 or claim 5, n the pellets comprise flour and/0r bran in addition to gluten. mm»: 1

7. A method as claimed in any one of ciaims 1 to 6, wherein the gluten containing plant material is obtained from wheat.

8. A method as claimed in any one of claims 1 to 7, wherein the fermented product of step c) is in the form of a solid or semi-solid cake.

9. A method as claimed in claim 8, wherein the cake is texturised to give a food product having a texture of minced meat.

10. A method as claimed in any one of claims 1 to 9, wherein the contact time in step a) is 16 to 24 hours.

11. A method as claimed in any one of claims 1 to 10, wherein the pH is reduced to or maintained at less than 5 by addition of an organic acid.

12. A method as claimed in claim 11, wherein the organic acid is lactic acid, citric acid, acetic acid or malic acid.

13. A method as claimed in any one of claims 1 to 12. wherein the heating temperature of step b) is in the range 100 to 130 “C and the heating time is 3 to 10 minutes.

14. A method as claimed in claim 13 n the heating temperature is 120°C.

15. A method as claimed in claim 13 wherein the heating time is 5 s.

16. A product obtained by the process of any one of claims 1 to 15.

17. The use of the food product of claim 16 as a vegetable-based replacement for minced ”(v-Wm meat.

18. A method for preparing a vegetable-based food product as d in claim 1. substantially as herein described with reference to any one of the ments of the invention illustrated in the accompanying examples but ing any comparative examples. it any.

19. Use as defined in claim 17, substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying examples but excluding any comparative examples, it any.