WO2013032011A1 - 糖鎖付加ポリペプチドおよび当該ポリペプチドを含む医薬組成物 - Google Patents

糖鎖付加ポリペプチドおよび当該ポリペプチドを含む医薬組成物 Download PDF

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WO2013032011A1
WO2013032011A1 PCT/JP2012/072380 JP2012072380W WO2013032011A1 WO 2013032011 A1 WO2013032011 A1 WO 2013032011A1 JP 2012072380 W JP2012072380 W JP 2012072380W WO 2013032011 A1 WO2013032011 A1 WO 2013032011A1
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Prior art keywords
glycosylated
amino acid
polypeptide
glycosylated polypeptide
srif28
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PCT/JP2012/072380
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English (en)
French (fr)
Japanese (ja)
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洋文 落合
泰治 下田
一博 深江
政敏 前田
一之 石井
健太 吉田
克成 手塚
圭亮 田鶴
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株式会社糖鎖工学研究所
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Priority to RU2014112046A priority Critical patent/RU2624034C2/ru
Priority to IN2399CHN2014 priority patent/IN2014CN02399A/en
Priority to CA2847334A priority patent/CA2847334C/en
Priority to BR112014004784A priority patent/BR112014004784A2/pt
Priority to KR1020147008130A priority patent/KR102031998B1/ko
Priority to EP12828271.2A priority patent/EP2752424B1/en
Priority to SG11201400289SA priority patent/SG11201400289SA/en
Priority to CN201280053754.2A priority patent/CN103930441B/zh
Application filed by 株式会社糖鎖工学研究所 filed Critical 株式会社糖鎖工学研究所
Priority to AU2012302636A priority patent/AU2012302636B2/en
Priority to JP2013531454A priority patent/JP6178238B2/ja
Priority to DK12828271T priority patent/DK2752424T3/da
Priority to US14/342,359 priority patent/US9422357B2/en
Publication of WO2013032011A1 publication Critical patent/WO2013032011A1/ja
Priority to US15/205,960 priority patent/US9937264B2/en

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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/655—Somatostatins
    • C07K14/6555—Somatostatins at least 1 amino acid in D-form
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C07K14/575—Hormones
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    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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    • A—HUMAN NECESSITIES
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    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/02—Drugs for disorders of the endocrine system of the hypothalamic hormones, e.g. TRH, GnRH, CRH, GRH, somatostatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention relates to a glycosylated polypeptide and a pharmaceutical composition containing the polypeptide.
  • Somatostatin is a cyclic peptide that exists in both the central nervous system and surrounding tissues. Somatostatin was first isolated from the mammalian hypothalamus and identified as an important inhibitor of growth hormone secretion from the anterior pituitary gland. This peptide is widely distributed in the hypothalamus, pancreas, gastrointestinal tract, and the like, and expresses an action through binding to a somatostatin receptor.
  • somatostatin suppresses growth hormone (GH) secretion in the pituitary gland, thyrotropin (TSH) secretion inhibition, gastrin in the gastrointestinal tract, selectin, cholecystokinin (CCK), VIP (Vasactive Intestinal Polypeptide), It is known to suppress secretion of various hormones such as glucagon and insulin in the pancreas. In addition, it is also known to have an action of suppressing gastrointestinal motility.
  • GH growth hormone
  • TSH thyrotropin
  • CCK cholecystokinin
  • VIP Vasactive Intestinal Polypeptide
  • Natural somatostatin (somatotropin release inhibitor (SRIF)) having the structural formula: Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys (SEQ ID NO: 1) was first isolated by Guillemin and collaborators. This somatostatin exerts its effects by interacting with a family of receptors.
  • the somatostatin receptor (SSTR) has 1 to 5 subtypes (SSTR1 to SSTR5).
  • SSTR2 is a GH-secreting human pituitary adenoma, central nervous system, anterior pituitary gland, retina, adrenal medulla, stomach It is known to be distributed in the duodenal mucosa, small intestine, colon tissues, and glucagon-secreting A cells of the pancreatic islets of Langerhans. Each of these receptors is also known to be expressed in various tumors. For example, SSTR1 and SSTR5 are expressed in functional pituitary adenomas, and SSTR1 in addition to SSTR2 in gastrointestinal tumors.
  • Non-Patent Document 1 SSTR3 is expressed, SSTR3 is expressed in pheochromocytoma, SSTR1 and SSTR5 are expressed in prostate cancer, and SSTR5 is expressed in colorectal cancer.
  • SSTR4 has been reported to function as a receptor having an antagonistic regulatory action and to be important in the treatment of glaucoma-related diseases (Non-patent Document 2).
  • somatostatin and its analogs are potentially useful therapeutic agents for somatostatin related diseases and various types of tumors.
  • somatostatin On the other hand, naturally occurring somatostatin has two undesirable properties of low bioavailability and short duration of action due to its short blood half-life of 2-3 minutes. Use and applications are limited. For this reason, various somatostatin analogs have been identified in order to find somatostatin analogs that are either superior in potency, biostability, duration of action, or selectivity considering the suppression of growth hormone, insulin or glucagon release. Development has been done.
  • Octreotide (Patent Document 1 and Patent Document 2) has been reported as the first approved somatostatin analog that can be clinically used, and this octreotide is associated with SSTR2, SSTR3, and SSTR5 of the somatostatin receptor. It is known to have an affinity for it.
  • Octreotide has a sequence of four amino acids (Phe-Trp-Lys-Thr), which is an important part for showing the biological activity of somatostatin, and has disulfide (SS) bonds at both ends of the sequence. It has been developed as a cyclic peptide consisting of 8 amino acids having Cys to form and further having D-Phe and Thr (ol) outside Cys at both ends.
  • This octreotide can have a sustained action by improving the blood half-life due to its amino acid sequence, and has a high selectivity against growth hormone (GH) compared to somatostatin and has a strong action. It is possible.
  • GH growth hormone
  • Somatostatin analogs containing such octreotide can be used to treat patients with hormone-secreting and hormone-dependent tumors.
  • symptoms associated with metastatic carcinoid tumors, neuroendocrine tumors (flushing, diarrhea, valvular disease and abdominal pain) and symptoms associated with vasoactive intestinal peptide (VIP) secretory adenomas (watery diarrhea) Is treated with octreotide.
  • VIP vasoactive intestinal peptide
  • octreotide inhibits both secretion and action of its active factor.
  • somatostatin analogs can reduce diarrhea by inhibiting VIP secretion and directly affecting intestinal secretion.
  • Non-patent Document 3 Non-patent Document 3
  • Octreotide is also used to treat acromegaly, but has been reported to be ineffective in about one third of patients with acromegaly.
  • octreotide is effective only in the initial stage of administration for carcinoid tumor patients, and tachyphylaxis (rapid growth tolerance, rapid growth tolerance) occurs when the administration period is prolonged.
  • octreotide has no effect on the suppression of adrenocorticotropic hormone (ACTH) production in patients with early Cushing's disease.
  • ACTH adrenocorticotropic hormone
  • somatostatin analogs that bind to multiple receptor subtypes with high affinity, such as natural somatostatin, is desired for tumors that express multiple somatostatin receptors.
  • the somatostatin analog having affinity for the somatostatin receptor may have an effect on patients who have no therapeutic effect or have resistance to conventional somatostatin analogs (non- Patent Document 4).
  • a somatostatin analog having a structure similar to that of naturally occurring somatostatin similarly having an affinity for the somatostatin receptor, and having an increased half-life in blood compared to somatostatin. It was.
  • An object of the present invention is to provide a glycosylated polypeptide having affinity for somatostatin receptor and having improved blood stability compared to somatostatin.
  • the present invention (A) SRIF14 consisting of the amino acid sequence represented by SEQ ID NO: 1; (B) polypeptide in which one or several amino acids are deleted, substituted or added in SRIF14 consisting of the amino acid sequence represented by SEQ ID NO: 1; C) an analog of SRIF14; (D) a polypeptide having 80% or more homology to SRIF14 consisting of the amino acid sequence represented by SEQ ID NO: 1; (E) N in (A) to (D) (Where N is an integer of 1 or more and 20 or less) a polypeptide further comprising N amino acids on the N-terminal side; and (F) In (A) to (D), M (where M is 1 or more and 6) A polypeptide further comprising the following integer) amino acids on the C-terminal
  • the amino acid substituted with the glycosylated amino acid is an amino acid corresponding to position 19 in SRIF14.
  • the glycosylated polypeptide is such that at least one amino acid in the polypeptide of (E) is substituted with a glycosylated amino acid, The at least one amino acid substituted with the glycosylated amino acid is present in any one of the N amino acids on the N-terminal side of the polypeptide of (E).
  • the glycosylated polypeptide is such that at least one amino acid in the polypeptide of (F) is substituted with a glycosylated amino acid, The at least one amino acid substituted with the glycosylated amino acid is present in any one of the M amino acids on the C-terminal side of the polypeptide (F).
  • the glycosylated polypeptide is such that at least one amino acid in the polypeptide of (E) is substituted with a glycosylated amino acid, ,
  • the sequence of the N amino acids added to the N-terminal side is represented by XY-, where X is a sequence of L amino acids (where L is an integer from 1 to 6)
  • Y is (1) Lys, (2) Arg-Lys, (3) Glu-Arg-Lys, (4) Arg-Glu-Arg-Lys, (5) Pro-Arg-Glu- Arg-Lys, (6) Ala-Pro-Arg-Glu-Arg-Lys, (7) Met-Ala-Pro-Arg-Glu-Arg-Lys, (8) Ala-Met-Ala-Pro-Arg-Glu -Lys, (9) Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys, (10) Asn-Pro-Ala-Met
  • At least one amino acid substituted with the glycosylated amino acid is added to the N-terminal side of the polypeptide of (E). It is characterized in that it is present in any one of L amino acids representing X in XY- representing the amino acid sequence of In one embodiment of the glycosylated polypeptide of the present invention, the N amino acids added to the N-terminal side are linked to the N-terminal side via a linker.
  • SRIF28 consisting of the amino acid sequence represented by SEQ ID NO: 2
  • B SRIF28 consisting of the amino acid sequence represented by SEQ ID NO: 2, 1 Or a polypeptide in which several amino acids are deleted, substituted or added
  • C an analog of SRIF28
  • D a homology of 80% or more with SRIF28 comprising the amino acid sequence represented by SEQ ID NO: 2
  • E In (E) (A) to (D), a polypeptide further comprising J amino acids (where J is an integer of 1 or more and 6 or less) on the N-terminal side
  • F (A) In (D), a polypeptide further comprising K amino acids (where K is an integer of 1 to 6) on the C-terminal side
  • a polypeptide selected from the group consisting of at least one amino acid is substituted with a glycosylated amino acid and has an affinity for a somato
  • the amino acid substituted with the glycosylated amino acid corresponds to the amino acid corresponding to position 1 in SRIF28, the amino acid corresponding to position 5, and the 9th position. It is at least one amino acid selected from the group consisting of an amino acid, an amino acid corresponding to the 12th position, an amino acid corresponding to the 13th position, an amino acid corresponding to the 14th position, and an amino acid corresponding to the 19th position.
  • the glycosylated polypeptide is such that at least one amino acid in the polypeptide of (E) is substituted with a glycosylated amino acid, The at least one amino acid substituted with the glycosylated amino acid is present in any one of the J amino acids on the N-terminal side of the polypeptide of (E).
  • the glycosylated polypeptide is such that at least one amino acid in the polypeptide of (F) is substituted with a glycosylated amino acid, The at least one amino acid substituted with the glycosylated amino acid is present in any one of the K amino acids on the C-terminal side of the polypeptide (F).
  • the affinity for the somatostatin receptor is at least two or more of receptors selected from the group consisting of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5. It has an affinity for the receptor.
  • the glycosylated polypeptide of the present invention has an affinity for at least one of SSTR1 and SSTR4. In one embodiment of the glycosylated polypeptide of the present invention, the glycosylated polypeptide has an affinity for both SSTR1 and SSTR4.
  • the glycosylated polypeptide has an affinity for all of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5. . In one embodiment of the glycosylated polypeptide of the present invention, the glycosylated polypeptide has an increased blood stability compared to SRIF28. In one embodiment of the glycosylated polypeptide of the present invention, each glycosylated amino acid is glycosylated Asn or glycosylated Cys.
  • each of the glycosylated amino acids is characterized in that the sugar chain and the amino acid are bound via a linker.
  • each glycosylated amino acid is characterized in that the sugar chain is composed of 4 or more sugars.
  • the sugar chain in each of the glycosylated amino acids, is a double-stranded complex type sugar chain, a triple-stranded complex type sugar chain, or a four-chain complex type. It is a sugar chain.
  • each of the glycosylated amino acids is characterized in that the sugar chain is a double-stranded complex type sugar chain.
  • the double-stranded complex type sugar chain is a group consisting of a disialo sugar chain, a monosialo sugar chain, an asialo sugar chain, a diglucnac sugar chain, and a dimannose sugar chain. It is characterized by being a sugar chain selected from.
  • the sugar chain in each glycosylated amino acid, is represented by the following formula: [Wherein R 1 and R 2 are the same or different, And Ac represents an acetyl group. ] It is the sugar chain represented by these.
  • the sugar chain has at least one sialic acid at the non-reducing end, and the carboxy group of the sialic acid has 1 to 30 carbon atoms. It is modified with an alkylamino group, a benzyl group, an amino group, or an aminoethylamino group.
  • the glycosylated polypeptide has a plurality of glycosylated amino acids, and each of the glycosylated amino acids has any sugar chain. It is characterized by being identical.
  • the glycosylated polypeptide has Cys at position 17 and Cys at position 28 in SRIF28, and these Cys is characterized by being disulfide bonded to each other.
  • the C-terminus of the glycosylated polypeptide is amidated.
  • an azide group is introduced at the N-terminus of the glycosylated polypeptide.
  • the glycosylated polypeptide is labeled.
  • another aspect of the present invention includes (I) a glycosylated polypeptide as described above and / or a pharmaceutically acceptable salt thereof, and (II) a pharmaceutically acceptable carrier. It is related with the pharmaceutical composition characterized by these. Moreover, in one embodiment of the pharmaceutical composition of the present invention, the sugar chain is substantially uniform in the glycosylated polypeptide. In one embodiment of the pharmaceutical composition of the present invention, the pharmaceutical composition is used for treatment or prevention of a disease related to somatostatin.
  • the somatostatin-related disease is acromegaly, giantism, Alzheimer's disease and other forms of dementia, cancer, hormone-producing tumor, endocrine tumor, carcinoid , VIPoma, insulinoma, glucagonoma, Cushing's disease, hormonal secretion disorder, diabetes and its complications, pain, arthritis, diarrhea, gastric ulcer, inflammatory bowel disease, irritable bowel syndrome, gastrointestinal obstruction, ileus, postoperative restenosis, It is characterized by being at least one disease selected from the group consisting of radiation injury, eye disease, dry eye, glaucoma, corneal inflammation, ulceris, cataract, and conjunctivitis.
  • the somatostatin-related disease is acromegaly, giantism, Alzheimer's disease and other forms of dementia, cancer, hormone-producing tumor, endocrine tumor, Carcinoid, VIP Oma, insulinoma, glucagonoma, Cushing's disease, hormonal secretion disorder, diabetes and its complications, pain, arthritis, diarrhea, gastric ulcer, inflammatory bowel disease, irritable bowel syndrome, gastrointestinal obstruction, ileus, postoperative restenosis And at least one disease selected from the group consisting of radiation injury, eye disease, dry eye, glaucoma, inflammation of the corneal stroma, ulceris, cataract, and conjunctivitis.
  • the glycosylated polypeptide of the present invention has affinity for somatostatin receptor and has improved blood stability compared to somatostatin.
  • the glycosylated polypeptide of the present invention has the above characteristics, and can be used for the treatment of somatostatin related diseases.
  • the sugar chain added to the sugar chain-added somatostatin of the present invention is easily decomposed in the living body, its accumulation does not cause phytotoxicity to the living body.
  • a part or all of the sugar chain added to the sugar chain-added somatostatin of the present invention is a sugar chain existing in a living body such as a mammal bird including humans or a modified body thereof, and even if administered in the body, it has a side effect. And is less likely to show antigenicity. There are few concerns about allergic reactions, antibody production, and inability to obtain medicinal effects.
  • FIG. 1A to FIG. 1D are diagrams showing each compound name and the amino acid sequence information of the glycosylated polypeptide having the compound name for the glycosylated polypeptide in one embodiment of the present invention.
  • FIG. 1E shows the IC 50 for cAMP production inhibitory action (agonist activity) when SRIF14, SRIF28, and the glycosylated polypeptide of one embodiment of the present invention were treated in somatostatin receptor-expressing cells. It is a graph which shows a value.
  • FIG. 2 is a graph showing changes in plasma concentration of each polypeptide when SRIF28 and S1C (disialo) -SRIF28 are intravenously or subcutaneously administered to rats.
  • S1C disialo
  • FIG. 2 shows changes in plasma concentration of each polypeptide when administered intravenously, and the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously.
  • FIG. 3 shows changes in plasma concentrations of SRIF28, S1C (disialo) -SRIF28, N5C (disialo) -SRIF28, and S1C (disialo) / N5C (disialo) -SRIF28 administered to rats intravenously and subcutaneously. It is a graph which shows. In FIG.
  • FIG. 4 shows intravenous and subcutaneous administration of SRIF28, S1C (disialo) -SRIF28, S1C (disialo), R13C (disialo) -SRIF28, S1C (disialo), N5C (disialo), A9C (disialo) -SRIF28 to rats. It is a graph which shows transition of the density
  • concentration in plasma at the time of doing.
  • FIG. 5 shows S1C (disialo) -SRIF28, N5C (disialo) -SRIF28, A9C (disialo) -SRIF28, S1C (disialo) ⁇ N5C (disialo) -SRIF28, N5C (disialo) ⁇ A9C (disialo, SRIF28SSR 2 is a graph showing changes in plasma concentration when (disialo) ⁇ N5C (disialo) ⁇ A9C (disialo) -SRIF28 was administered intravenously and subcutaneously to rats.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously.
  • FIG. 6 is a graph showing changes in plasma concentrations when S1C (disialo) -SRIF28, S1-2C (disialo) -SRIF28, and S1-3C (disialo) -SRIF28 were administered intravenously and subcutaneously to rats. It is.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 7 shows changes in plasma concentrations of S1C (disialo) -SRIF28, S1C (disialo (amide))-SRIF28, and S1C (disialo (aminoethylamide))-SRIF28 in rats after intravenous and subcutaneous administration. It is a graph to show.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 8 shows changes in plasma concentrations of S1C (disialo) -SRIF28, S1C (disialo) -D-Trp22-SRIF28, and S1C (disialo (Bn))-SRIF28 administered intravenously and subcutaneously to rats. It is a graph which shows. In FIG. 8, the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously, and the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is. FIG.
  • FIG. 9 is a graph showing changes in plasma concentration when S1C (disialo) -SRIF28, R13C (disialo) -SRIF28, and K14C (disialo) -SRIF28 are intravenously and subcutaneously administered to rats.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 10 shows S1C (disialo) -SRIF28, E12C (disialo) -SRIF28, N19C (disialo) -SRIF28, 29C (disialo) -SRIF28, S1C (monosialo) -SRIF28, S1C (asialo) -SRIF28 intravenously It is a graph which shows transition of the density
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 10 shows S1C (disialo) -SRIF28, E12C (disialo) -SRIF28, N19C (disialo) -SRIF28, 29C (disialo) -SRIF28, S1C (monosi
  • FIG. 11 is a graph showing changes in plasma concentrations when S1C (disialo) -SRIF28, K14C (disialo) -SRIF28, and C (disialo) -SRIF14 are intravenously and subcutaneously administered to rats.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 12 is a graph showing changes in plasma concentrations of C (disialo) -SRIF14, C (disialo) -C12linker-SRIF14, and C (disialo) -PEGlinker-SRIF14 administered intravenously and subcutaneously to rats. It is.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 12 is a graph showing changes in plasma concentrations of C (disialo) -SRIF14, C (disialo) -C12linker-SRIF14, and C (disialo) -PEGlinker-SRIF14 administered intravenously and subcutaneously to rats. It is.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each
  • FIG. 13 shows that SRIF28, S1C (disialo) -SRIF28, S1C (asialo) -SRIF28, S1C (diGlcNAc) -SRIF28, S1C (diMan) -SRIF28, S1C (GlcNAc) -SRIF28 were administered intravenously and subcutaneously to rats. It is a graph which shows transition of the density
  • FIG. 13 shows that SRIF28, S1C (disialo) -SRIF28, S1C (asialo) -SRIF28, S1C (diGlcNAc) -SRIF28, S1C (diMan) -SRIF28, S1C (GlcNA
  • FIG. 14 shows intravenous and subcutaneous administration to S1C (disialo) -SRIF28, S1C (trisialo) -SRIF28, S1C (tetrasial) -SRIF28, S1-2C (disialo) -SRIF28, S1C (Asn (disialo))-SRIF28 rats.
  • It is a graph which shows transition of the density
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 14 shows intravenous and subcutaneous administration to S1C (disialo) -SRIF28, S1C (trisialo) -SRIF28, S1C (tetrasial) -SRIF28, S1-2C (disialo) -SRIF28, S1C (
  • FIG. 15 shows that SRIF28, S1C (disialo) -SRIF28, S1-2C (disialo) -SRIF28, S1-3C (disialo) -SRIF28, S1-4C (disialo) -SRIF28 were administered intravenously and subcutaneously to rats. It is a graph which shows transition of the density
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 15 shows that SRIF28, S1C (disialo) -SRIF28, S1-2C (disialo) -SRIF28, S1-3C (disialo) -SRIF28, S1-4C (disialo) -SRIF28 were administered intravenously and subcutaneously to rats. It is a graph which shows transition of the density
  • FIG. 16 shows SRIF14, C (disialo) -SRIF14, C (disialo) -K-SRIF14, C (disialo) -RK-SRIF14, 2C (disialo) -RK-SRIF14, 3C (disialo) -R -Is a graph showing changes in plasma concentration when K-SRIF14 was administered intravenously and subcutaneously to rats.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 17 is a graph showing changes in plasma concentrations when S1C (asialo) -SRIF28, S1-2C (asialo) -SRIF28, and S1-3C (asialo) -SRIF28 were intravenously and subcutaneously administered to rats. It is.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 18 shows S1C (disialo) -SRIF28, S1-2C (disialo) -SRIF28, S1-2C (asialo) -SRIF28, S1-2C (disialo (amide))-SRIF28, S1-2C (disialo (Bn)) -SRIF28, C (disialo (aminoethylamide))-S1C (disialo) -SRIF28 is a graph showing changes in plasma concentration when administered intravenously and subcutaneously to rats.
  • the left graph is a graph showing changes in plasma concentration of each polypeptide when administered intravenously
  • the right graph is a graph showing changes in plasma concentration of each polypeptide when administered subcutaneously. It is.
  • FIG. 19 is a graph showing the results of a plasma stability test using rat plasma for a glycosylated polypeptide according to one embodiment of the present invention.
  • somatostatin refers to SRIF14 consisting of a 14 amino acid sequence or SRIF28 consisting of a 28 amino acid sequence.
  • the N-terminal Ser in the amino acid sequence of SRIF28 is defined as position 1
  • the C-terminal Cys is defined as position 28.
  • SRIF14 completely coincides with the amino acid sequence at positions 15 to 28 in the amino acid sequence of SRIF28.
  • the 15th position of the amino acid sequence of SRIF28 is Ala
  • the N-terminal Ala in the amino acid sequence of SRIF14 corresponds to the 15th position of SRIF28 and becomes the 15th position.
  • SRIF14 and SRIF28 have a disulfide bond at Cys at position 17 and Cys at position 28.
  • SRIF14 has the following amino acid sequence (SEQ ID NO: 1).
  • Ala 15 ” 15 means Ala at position 15.
  • SRIF28 has the following amino acid sequence (SEQ ID NO: 2). Ser 1 -Ala 2 -Asn 3 -Ser 4 -Asn 5 -Pro 6 -Ala 7 -Met 8 -Ala 9 -Pro 10 -Arg 11 -Glu 12 -Arg 13 -Lys 14 -Ala 15 -Gly 16 -Cys 17 -Lys 18 -Asn 19 -Phe 20 -Phe 21 -Trp 22 -Lys 23 -Thr 24 -Phe 25 -Thr 26 -Ser 27 -Cys 28
  • amino acid is used in its broadest sense and includes not only natural amino acids but also non-natural amino acids such as amino acid variants and derivatives.
  • amino acids herein, for example, natural proteogenic L-amino acids; D-amino acids; chemically modified amino acids such as amino acid variants and derivatives; norleucine, It will be understood that natural non-proteogenic amino acids such as ⁇ -alanine, ornithine; and chemically synthesized compounds having properties known in the art that are characteristic of amino acids, and the like.
  • unnatural amino acids examples include ⁇ -methyl amino acids (such as ⁇ -methylalanine), D-amino acids, histidine-like amino acids (2-amino-histidine, ⁇ -hydroxy-histidine, homohistidine, ⁇ -fluoromethyl-histidine and ⁇ -Methyl-histidine, etc.), amino acids with extra methylene in the side chain (“homo" amino acids) and amino acids in which the carboxylic acid functional amino acids in the side chain are substituted with sulfonic acid groups (such as cysteic acid).
  • somatostatin analogs that have affinity for the somatostatin receptor are known to contain unnatural amino acids.
  • the amino acid contained in the compound of the present invention consists only of natural amino acids.
  • the number of substituted amino acids is not particularly limited as long as the affinity for the somatostatin receptor is maintained. 1 to 9, preferably 1 to 5, more preferably about 1 to 3, or within 20%, preferably within 10% of the total length.
  • the amino acid to be substituted or added can be a natural amino acid, a non-natural amino acid or an amino acid analog, preferably a natural amino acid.
  • somatostatin peptides in which one or several amino acids are deleted, substituted, or added include, for example, somatostatin peptide in which Trp at position 22 is replaced with D-form Trp (D-Trp), Asn at position 19 (J. Med. Chem, 2001, 44, 2238-2246), Phe at position 25 replaced with Tyr, Met at position 8 replaced with Leu (Endocrinology, 1982, 10: 1049-1051) and the like.
  • an analog of SRIF14 or SRIF28 refers to a polypeptide structurally similar to somatostatin and / or a polypeptide having a structure overlapping with somatostatin, for example, one or several amino acids of somatostatin amino acids. Substituted polypeptides, somatostatin variants, fragments of somatostatin with affinity for somatostatin receptors, extended somatostatin with affinity for somatostatin receptors.
  • one or several amino acids of an amino acid are conservatively substituted means that the hydrophilicity index and / or the hydrophobicity index of an amino acid substituted with the original amino acid in amino acid substitution is Similar substitutions that refer to substitutions that do not cause a clear decrease or loss of affinity for the somatostatin receptor before and after such substitutions.
  • somatostatin variant refers to a variant of somatostatin, which includes a naturally occurring variant of somatostatin, or a compound obtained by artificially modifying somatostatin.
  • alkylation e.g acetylation
  • acylation e.g acetylation
  • amidation carboxylation
  • ester formation e.g., glutathione
  • disulfide bond formation e.g., glutasylation
  • glycosylation lipidation
  • phosphorylation e.g., hydroxylation
  • labeling of one or more amino acid residues of somatostatin include binding of components.
  • a fragment of somatostatin having affinity for a somatostatin receptor means that one or more amino acids are deleted from the N-terminal and / or C-terminal of somatostatin, and the affinity for somatostatin receptor. It is a peptide that maintains sex.
  • extended somatostatin having affinity for somatostatin receptor means that one or more amino acids are added to the N-terminal and / or C-terminal of SRIF28 or SRIF14, and affinity for somatostatin receptor. It is a peptide that maintains sex.
  • the glycosylated polypeptide of the present invention comprises a polypeptide comprising an amino acid sequence having 80% or more homology with the amino acid sequence represented by SEQ ID NO: 1, and 80% or more homology with the amino acid sequence represented by SEQ ID NO: 2.
  • the polypeptide having homology with SEQ ID NO: 1 or SEQ ID NO: 2 preferably has a homology of 80% or more, 85% or more, 90% or more, 95% or more as long as it has affinity for the somatostatin receptor. Can do.
  • the glycosylated polypeptide of the present invention includes a polypeptide in which an amino acid is further added to the N-terminus and / or C-terminus of SRIF14 or SRIF28.
  • the amino acid further added to the N-terminus of SRIF14 is not particularly limited as long as the affinity for the somatostatin receptor is maintained. For example, 1 to 20 amino acids are added. Can do.
  • the amino acid further added to the N-terminus of SRIF14 can be represented by XY-.
  • Y is an amino acid that directly binds to the N-terminal amino acid in the SRIF14 polypeptide, and Y consists of any of the following amino acid sequences (1) to (15): (1) Lys, (2) Arg-Lys, (3) Glu-Arg-Lys, (4) Arg-Glu-Arg-Lys, (5) Pro-Arg-Glu-Arg-Lys, (6) Ala-Pro-Arg-Glu-Arg-Lys, (7) Met-Ala-Pro-Arg-Glu-Arg-Lys, (8) Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys, (9) Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys, (10) Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys, (11) Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys, (12) Asn-Ser-Asn-Pro-Ala-Met
  • the amino acid further added to the N-terminus of SRIF28 is not particularly limited as long as the affinity for the somatostatin receptor is maintained, but for example, any amino acid of 1 to 6 is added. Any one, two, three, four, five, or six amino acids can be added.
  • the amino acid further added to the N-terminus of SRIF28 is preferably a glycosylated amino acid, more preferably a glycosylated Asn or a glycosylated Cys.
  • all of the arbitrary amino acids of 1-6 can also be made into a glycosylated amino acid.
  • the amino acid further added to the C-terminus of SRIF14 or SRIF28 is not particularly limited as long as the affinity for the somatostatin receptor is maintained.
  • any amino acid of 1 to 6 any one, two, three, four, five, or six amino acids can be added.
  • the amino acid further added to the C-terminus of SRIF14 or SRIF28 is preferably a glycosylated amino acid, more preferably a glycosylated Asn or a glycosylated Cys.
  • all of the arbitrary amino acids of 1-6 can also be made into a glycosylated amino acid.
  • SRIF28 is N-terminal 1
  • An arbitrary amino acid or a glycosylated amino acid is further added to Ser at the position.
  • SRIF14 it refers to an N-terminal 15-position Ala to which any amino acid or glycosylated amino acid has been added.
  • any amino acid or sugar chain is added to Cys at position 28 of SRIF28 or SRIF14. This refers to the addition of an additional amino acid.
  • an amino acid can be further added to the N-terminus or C-terminus via a linker.
  • a linker include an alkyl chain and a polyethylene glycol chain having an amino group and a carboxy group at both ends so that an amino acid and a peptide can be bonded.
  • Such a linker is, for example, —NH— (CH 2 ) n —CO— (wherein n is an integer and is not limited as long as the desired linker function is not inhibited, but preferably 1 Represents an integer of ⁇ 15) or —NH— (CH 2 CH 2 O) m —CH 2 CH 2 —CO— (wherein m is an integer and is limited as long as the desired linker function is not inhibited) Although it is not a thing, Preferably it shows the integer of 1-7.) Etc. can be mentioned.
  • —NH— (CH 2 ) 11 —CO— (C12 linker), —NH— (CH 2 CH 2 O) 3 —CH 2 CH 2 —CO— (PEG linker), and the like can be given.
  • the amino acid added to the glycosylated polypeptide via the linker is not particularly limited, but is preferably a glycosylated amino acid.
  • examples of the sugar chain-added amino acid include sugar chain-added Asn and sugar chain-added Cys.
  • an azide group can also be introduced into the N-terminus of the glycosylated polypeptide.
  • the N-terminus of the glycosylated polypeptide is azylated, it is preferable because various molecules can be selectively introduced using an azido-alkyne [3 + 2] cycloaddition reaction or Staudinger reaction.
  • the method for azating a glycosylated polypeptide is not particularly limited, but for example, by condensing an azide-substituted fatty acid with a condensing agent to the N-terminus of a glycopeptide synthesized on a resin during solid phase synthesis. Obtainable.
  • the azide-substituted fatty acid include 4-azidobutanoic acid, 5-azido-pentanoic acid, and 6-azidohexanoic acid.
  • the glycosylated polypeptide can be added with a labeling compound at its N-terminus.
  • the labeling compound used in the present invention include, but are not limited to, biotin, fluorescent dyes, metal ion chelating agents, and the like.
  • the labeling compound can be directly bonded to the N-terminus of the glycopeptide, or can be bonded to the N-terminus of the glycopeptide via a linker.
  • biotin as a labeling compound to the N-terminus of a glycopeptide, it can be applied to research reagents, clinical diagnostics, or missile therapy using a strong bond with avidin. .
  • the labeling compound can be added to the glycosylated polypeptide of the present invention by methods well known to those skilled in the art.
  • the labeling compound can be condensed using a condensing agent at the N-terminus of the glycosylated polypeptide on the resin during solid phase synthesis.
  • the “glycosylated polypeptide” of the present invention is characterized in that at least one amino acid is substituted with a glycosylated amino acid.
  • the “glycosylated polypeptide” includes a polypeptide in which at least one amino acid of somatostatin is substituted with a glycosylated amino acid, and at least one amino acid in the above somatostatin analog is a glycosylated amino acid.
  • the glycosylated polypeptide of the present invention even if one or several amino acids other than the glycosylated amino acid are deleted, substituted or added, respectively. It is.
  • Peptides in which the C-terminus of these peptides is amidated eg, Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-NH 2 (SEQ ID NO: 3)
  • SRIF14NH 2 having the amino acid sequence of Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe
  • a peptide in which at least one amino acid of SRIF28NH 2 having the amino acid sequence of -Trp-Lys-Thr-Phe-Thr-Ser-Cys-NH 2 (SEQ ID NO: 4) is substituted with a glycosylated amino acid is also included in the present invention
  • the salts of these peptides are also included in Included in the chain
  • the salt may be either an acid addition salt or a base addition salt.
  • Acids commonly used to form acid addition salts include inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, phosphoric acid, and p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p- Organic acids such as bromophenyl sulfonic acid, carboxylic acid, succinic acid, citric acid, benzoic acid and acetic acid.
  • Base addition salts include ammonium hydroxide or salts derived from inorganic bases such as alkali or alkaline earth metal hydroxides, carbonates, bicarbonates and the like. In particular, a pharmaceutically acceptable salt is preferable.
  • the “glycosylated amino acid” is an amino acid to which a sugar chain is bound, and the sugar chain and the amino acid may be bound via a linker.
  • the amino acid is bound to the reducing end of the sugar chain.
  • the type of amino acid to which the sugar chain is bound and either a natural amino acid or a non-natural amino acid can be used.
  • the glycosylated amino acid has the same or similar structure as that present as a glycopeptide (glycoprotein) in the living body
  • the glycosylated amino acid is a glycosylated Asn such as an N-linked sugar chain.
  • Glycosylated Ser such as O-linked sugar chain and glycosylated Thr are preferable, and glycosylated Asn is particularly preferable.
  • the amino acid of the sugar chain-added amino acid has two or more amino acids in the molecule such as aspartic acid and glutamic acid.
  • Amino acids with carboxy groups, lysine, arginine, histidine, tryptophan, etc. amino acids with two or more amino groups, serine, threonine, tyrosine, etc.
  • amino acids having an amide group in the molecule such as asparagine and glutamine.
  • aspartic acid, glutamic acid, lysine, arginine, serine, threonine, cysteine, asparagine, and glutamine are preferable.
  • the glycosylated amino acid is a sugar chain. It is considered that there is no significant difference in the half-life in blood of the glycosylated polypeptide of the present invention between the case of added Asn (not via a linker) and the case of glycosylated Cys (via a linker).
  • a sugar chain and an amino acid are bonded via a linker
  • those used in the art can be widely used.
  • a is an integer and is not limited as long as the desired linker function is not inhibited, but preferably represents an integer of 0 to 4
  • C 1-10 polymethylene —CH 2 —R—, wherein R is alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, carbocyclic group, substituted carbocycle A group formed by elimination of one hydrogen atom from a group selected from the group consisting of a group, a heterocyclic group and a substituted heterocyclic group), — (CO) — (CH 2 ) a — (CO) — (formula A is an integer, and is not limited as long as the desired linker
  • the linker when the sugar chain and the amino acid on the somatostatin skeleton are bonded via a linker, the linker preferably includes an amino acid at the sugar chain side end.
  • the type of amino acid is not particularly limited, but a preferred example is Asn.
  • the production method of the glycosylated polypeptide of the present invention is not limited by the description (for example, “description of a glycosylated polypeptide in which an amino acid is substituted with a glycosylated amino acid”).
  • the glycosylated polypeptide produced by any of the methods A or B is included in the “glycosylated polypeptide in which an amino acid is substituted with a glycosylated amino acid”.
  • a glycosylated polypeptide in which a sugar chain to which no amino acid is bonded is bound to an amino acid on a peptide directly or via a linker; in a glycosylated polypeptide, a sugar or a sugar chain is further added to the added sugar chain
  • a glycosylated polypeptide obtained by extending a sugar chain that has already been added by adding 1 or several amino acids to the amino group and / or carboxy group of the glycosylated amino acid, and Glycosylated polypeptides linked to multiple somatostatin fragments; Glycosylated polypeptides in which an amino acid-linked sugar chain is linked to an amino acid on the peptide via a linker, etc. also have the same final structure. As long as it is included in the glycosylated polypeptide of the present invention.
  • the number of amino acids substituted with a glycosylated amino acid is present in the final glycosylated polypeptide, physiological activity such as blood stability and suppression of secretion of growth hormone, etc. What is necessary is just to adjust suitably according to the number of amino acids, the molecular weight of the glycosylated polypeptide before and after glycosylation, and the like.
  • 1 to 10 are preferably substituted, 1 to 5 are more preferably substituted, and 1 to 3 are more preferably substituted. From the viewpoint of simplicity, it is preferable to select one substitution if the desired activity can be obtained with one substitution.
  • two or more are preferably substituted, for example, 2 to 5 are preferably substituted, and 2 to 3 are more preferably substituted.
  • 2 to 5 are preferably substituted
  • 2 to 3 are more preferably substituted.
  • a glycosylated polypeptide in which one amino acid of somatostatin is substituted with a glycosylated amino acid when one or more amino acids other than the glycosylated amino acid are further substituted with a glycosylated amino acid, stability in blood Tends to increase and the affinity for the receptor tends to decrease.
  • the glycosylated polypeptide has increased blood stability, which can compensate or increase the decreased somatostatin activity.
  • the sugar chains can be added to consecutive amino acids in the amino acid sequence of the glycosylated polypeptide or added to the amino acids at intervals. You can also It is preferable to arrange sugar chains densely in that there is no sudden increase in plasma concentration. Further, from the viewpoint of bioavailability, it is preferable to add a plurality of sugar chains at intervals rather than densely adding consecutive sugar chains to the position where sugar chains are added.
  • the site where an amino acid is substituted with a glycosylated amino acid can be appropriately adjusted, particularly from the viewpoint of affinity for a plurality of somatostatin receptors.
  • the site at which an amino acid is substituted with a glycosylated amino acid has affinity for a plurality of SSTR1 to SSTR5 receptors among the affinity of a glycosylated polypeptide for a somatostatin receptor.
  • the amino acid corresponding to position 19 in SRIF14 the amino acid further added to the N-terminal side of SRIF14, the first, second, third, sixth, tenth, and fourteenth positions from the N-terminal side
  • amino acids that are further added to the N-terminal side of SRIF14 include the third, sixth, tenth, and fourteenth amino acids from the N-terminal side.
  • amino acids that are added first and second from the C-terminal side among the amino acids further added to the C-terminus of SRIF28 Preferably, amino acids corresponding to positions 1, 5, 9, and 12 of SRIF28 can be mentioned.
  • the glycosylated polypeptide of the present invention has affinity for a plurality of somatostatin receptors
  • the amino acid further added to the N-terminal side of SRIF14 the amino acid added to the 10th and 14th positions from the N-terminal side
  • the amino acid further added to the N-terminal side of SRIF14 added to the 6th and 10th positions from the N-terminal side
  • An amino acid further added to the N-terminal side of SRIF14 an amino acid added to the second and 14th positions from the N-terminal side
  • 15th amino acid added; further to the N-terminal side of SRIF14 Amino acids added at the 14th, 15th and 16th positions from the N-terminal
  • the amino acid added to the 10th and 14th positions from the N-terminal side in the amino acid further added to the N-terminal side of SRIF14, the amino acid added to the 10th and 14th positions from the N-terminal side; in the amino acid further added to the N-terminal side of SRIF14, the 6th and 10th positions from the N-terminal side Amino acids added to the N-terminal side of SRIF14, amino acids added to the 14th and 15th positions from the N-terminal side; amino acids further added to the N-terminal side of SRIF14, 14 amino acids from the N-terminal side Amino acids added to the Nth, 15th, and 16th amino acids; substitution of amino acids added to the 6th, 10th, and 14th amino acids from the N-terminal side of SRIF14 Can do.
  • amino acids corresponding to position 5 amino acid corresponding to positions 5 and 9; amino acid corresponding to positions 1 and 13; amino acid corresponding to position 1 and amino acids further added to the N-terminal side of position 1 from the N-terminal side
  • Examples include substitution of amino acids corresponding to the 9th position, preferably substitution of amino acids corresponding to the 1st and 5th positions; substitution of amino acids corresponding to the 5th and 9th positions; Substitution of the corresponding amino acid and the first amino acid added in the amino acid further added to the N-terminal side of the 1st position; 1 amino acid from the N-terminal side in the amino acid corresponding to
  • the site at which an amino acid is substituted with a glycosylated amino acid is, for example, an amino acid corresponding to position 19 in SRIF14, or the N-terminus of SRIF14 from the viewpoint of blood stability of the glycosylated polypeptide.
  • the amino acids to be added the first, second, third, sixth, tenth and fourteenth amino acids added from the N-terminal side, and the additional amino acids added to the C-terminal side of SRIF14 are 1 from the C-terminal side.
  • the amino acid added to the 2nd and 2nd can be mentioned.
  • amino acids to be added are the first and second amino acids from the C-terminal side. More preferably, an amino acid corresponding to position 19 in SRIF14, an amino acid added first from the N-terminal side among amino acids further added to the N-terminal side of SRIF14, and an amino acid further added to the C-terminal side of SRIF14 Is the first amino acid added from the C-terminal side.
  • the glycosylated polypeptide corresponds to the 1st, 5th, 9th, 12th, 13th, 14th, and 19th positions in SRIF28.
  • amino acid corresponding to a specific amino acid refers to an amino acid at the same position corresponding to the amino acid sequence of SRIF14 or SRIF28 unless an amino acid is added or deleted in the sugar chain polypeptide.
  • amino acid addition or deletion when an amino acid addition or deletion is present in the amino acid sequence of the sugar chain polypeptide, it means an amino acid at a position that takes into account movement on the amino acid sequence due to the addition or deletion of the amino acid.
  • one amino acid (Trp) is added between the 2nd and 3rd amino acids.
  • the amino acid corresponding to the amino acid at position 3 is an amino acid that has moved one by one to the C-terminal side due to the insertion of Trp in the glycosylated polypeptide. (Asn).
  • the amino acid substituted with a glycosylated amino acid is one or more amino acids selected from amino acids other than the amino acids corresponding to positions 17, 21, 22, 23, and 28 in SRIF28
  • one or more amino acids selected from amino acids other than the amino acids corresponding to positions 17, 22, 23, and 28 in SRIF28 are more preferable.
  • any combination of the above can be adopted as the site where amino acids are substituted with glycosylated amino acids. It is not limited. For example, a combination in which one site is selected from the above preferred sites and the other site is selected from any site of SRIF14 or SRIF28; one site is selected from the above preferred sites and the other site is N of somatostatin Combinations selected from the terminal (position 1 in SRIF28 or position 15 in SRIF14) or any part of one or several amino acids further added to the C-terminal are also included in a preferred embodiment of the present invention. .
  • the site where deletion, substitution or addition of amino acids other than glycosylated amino acids occurs is, for example, from amino acids other than the amino acids corresponding to positions 17, 22, 23, and 28 in SRIF28.
  • One or more selected amino acids are preferred.
  • sugar chain refers to a compound in which one or more unit sugars (monosaccharides and / or derivatives thereof) are linked. When two or more unit sugars are connected, each unit sugar is bound by dehydration condensation by a glycosidic bond.
  • sugar chains include monosaccharides and polysaccharides (glucose, galactose, mannose, fucose, xylose, N-acetylglucosamine (GlcNAc), N-acetylgalactosamine (GalNAc), sialic acid contained in the living body.
  • sugar chains that are decomposed or derived from complex biomolecules such as degraded polysaccharides, glycoproteins, proteoglycans, glycosaminoglycans, glycolipids, etc. It is not limited to.
  • the sugar chain may be linear or branched.
  • sugar chain also includes sugar chain derivatives.
  • sugar chain derivatives include sugars having a carboxy group (for example, C-1 Oxidized aldonic acid converted to carboxylic acid (for example, D-gluconic acid oxidized D-glucose), uronic acid whose terminal C atom became carboxylic acid (D-glucose oxidized D-glucose) Glucuronic acid)), sugars having amino groups or derivatives of amino groups (eg acetylated amino groups) (eg N-acetyl-D-glucosamine, N-acetyl-D-galactosamine etc.), amino groups and carboxy Sugars having both groups (eg, N-acetylneuraminic acid (sialic acid), N-acetylmuramic acid, etc.), deoxygenated sugars (eg, 2-deoxy-D-ribo Scan), sulfated sugar including a sulfuric acid group, including but sugar chains are such
  • a preferred sugar chain when added to somatostatin (when substituted with an amino acid of somatostatin in the form of a glycosylated amino acid), the blood stability of somatostatin is increased and the somatostatin receptor is added. It is a sugar chain that does not lose affinity.
  • a preferred sugar chain is a sugar that can maintain the affinity of somatostatin for multiple receptors when added to somatostatin (when substituted with an amino acid of somatostatin in the form of a glycosylated amino acid). More preferably, it is a sugar chain that can maintain affinity for all receptors of somatostatin.
  • the sugar chain in the glycosylated polypeptide of the present invention is not particularly limited, and may be a sugar chain that exists as a complex carbohydrate (glycopeptide (or glycoprotein), proteoglycan, glycolipid, etc.) in vivo, It may be a sugar chain that does not exist as a complex carbohydrate in vivo.
  • a sugar chain that exists as a complex carbohydrate in a living body is preferable from the viewpoint that the glycosylated polypeptide of the present invention is administered to a living body.
  • sugar chains include N-linked sugar chains and O-linked sugar chains that are sugar chains bound to peptides (or proteins) as glycopeptides (or glycoproteins) in vivo.
  • an N-linked sugar chain is used.
  • the N-linked sugar chain include a high mannose type, a complex type, and a hybrid type, and a complex type is particularly preferable.
  • Preferred complex type sugar chains used in the present invention include, for example, the following general formula [Wherein R 1 and R 2 are the same or different, Indicates. Ac represents an acetyl group. ] And the like.
  • the sugar chain in the glycosylated polypeptide of the present invention, even if the sugar chain exists as a complex carbohydrate in vivo, it binds to the somatostatin peptide by a method other than the O-linked type and the N-linked type. It may be.
  • those having a sugar chain bonded to Cys or Lys via a linker are also included in the glycosylated polypeptide of the present invention.
  • the sugar chain in the glycosylated polypeptide of the present invention is a sugar chain composed of 4 or more, for example, 5 or more, 7 or more, particularly 9 or more, 11 or more sugars. Is preferred.
  • the sugar chain in the glycosylated polypeptide of the present invention is a sugar chain composed of 5 to 11, 9 to 11, or 11 sugars.
  • the sugar chain in the glycosylated polypeptide of the present invention is a double-chain complex type sugar chain.
  • the complex-type sugar chain includes two or more types of monosaccharides, and has a basic structure shown below and a lactosamine structure represented by Gal ⁇ 1-4GlcNAc.
  • the double-stranded complex type sugar chain refers to one in which a single-chain sugar chain composed of 0 to 3 sugars is bonded to two mannoses at the end of the basic structure.
  • the complex sugar chain of the present invention includes the above-described double-chain complex sugar chain, a three-chain complex sugar chain (three-branch complex sugar chain), and a four-chain complex sugar chain. (4-branch complex type sugar chain) is also included.
  • examples of the three-chain complex sugar chain and the four-chain complex sugar chain also include sugar chains that have lost one or more sugars from the non-reducing end of these trisialo sugar chains or tetrasialo sugar chains.
  • the complex type sugar chain of the present invention includes those with fucose.
  • a fucose-containing complex type sugar chain represented by the following structural formula: Can be mentioned.
  • sugar chains in which one or more sugars have been lost from the non-reducing end of these fucose-containing complex sugar chains can also be mentioned.
  • “disialo sugar chain”, “monosialo sugar chain”, “asialo sugar chain”, “diglucnac sugar chain”, “dimannose sugar chain”, “double-stranded complex sugar chain”, “three” “Chain complex type sugar chain”, “Four chain complex type sugar chain” and “Fucose-containing complex type sugar chain” include those shown in the above chemical formula, and those having different binding modes from the examples shown in the chemical formula.
  • Such a sugar chain is also preferably used as the sugar chain of the present invention.
  • Examples of such sugar chains include those in which sialic acid and galactose are bonded by an ( ⁇ 2 ⁇ 3) bond in a dicialosaccharide chain or a monosialosugar chain.
  • a sugar chain in which the carboxy group of sialic acid is modified can also be used.
  • the modification of the carboxy group of sialic acid is preferably a group capable of eliminating the negative charge of the carboxy group or converting it to a positive charge, such as an alkylamino group having 1 to 30 carbon atoms, a benzyl group, an amino group And aminoethylamino group.
  • the negative charge of the carboxyl group of sialic acid is eliminated (benzyl group, amino group, etc.) or converted to a positive charge (aminoethylamino group, etc.), so that the glycosylated polypeptide Can be intended for control of blood clearance and body distribution.
  • the high mannose type sugar chain used in the present invention is a sugar chain in which two or more mannoses are further bonded to the basic structure of the complex type sugar chain described above. Since the high mannose type sugar chain is bulky, the stability in blood can be increased by binding the high mannose type sugar chain to the peptide.
  • a sugar chain containing 5 to 9 mannose is preferable like a mammalian high mannose sugar chain, but a sugar chain containing more mannose may be used like a high mannose sugar chain of yeast.
  • a high mannose type sugar chain preferably used in the present invention, for example, Highman North-5 (M-5) Highman North-9 (M-9) Etc.
  • preferable sugar chains include, for example, sugar chains existing as glycoproteins bound to proteins in the human body (for example, sugar chains described in “FEBS LETTERS Vol. 50, No. 3, Feb. 1975”). ) And a sugar chain having the same structure (a sugar chain having the same kind of constituent sugars and their coupling mode) or a sugar chain that has lost one or more sugars from the non-reducing end thereof. Examples include sugar chains.
  • the sugar chain structures of the glycosylated amino acids in the glycosylated polypeptide of the present invention can be substantially the same or have different sugar chain structures. Also good.
  • the sugar chain structure in the glycosylated polypeptide is substantially the same, for example, it is preferably 90% or more, or 99% or more, and the sugar chain structure is completely the same. Is most preferred.
  • the same sugar chain structure in a glycopeptide means that in a glycosylated polypeptide to which two or more sugar chains are added, the sugar chain is compared when the sugar chains are compared.
  • the sugar chain of the glycosylated polypeptide of the present invention is preferably uniform.
  • the sugar chain in the glycosylated polypeptide is uniform means that the sugar chain is compared between the glycosylated polypeptides, the sugar chain addition site in the peptide, and the type of each sugar constituting the sugar chain. That the order of linkage and the linkage mode between sugars are the same among glycopeptides, and that the structure of the sugar chain is at least 90% or more, preferably 95% or more, more preferably 99% or more.
  • a composition containing a glycopeptide having a uniform sugar chain among glycopeptides has a constant quality, and is particularly preferable in the fields of pharmaceutical production and assay.
  • the uniform sugar chain ratio can be measured, for example, by a method using HPLC, capillary electrophoresis, NMR, mass spectrometry or the like.
  • glycosylated polypeptides include, for example, glycosylated polypeptides (SEQ ID NOs: 5-37, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 110, 112, 114, 116, 118, 120, 123 to 129, 133 to 135, 138 to 141, 148, 155, 160).
  • amino acid sequence of SRIF14 In Ala 15 -Gly 16 -Cys 17 -Lys 18 -Asn 19 -Phe 20 -Phe 21 -Trp 22 -Lys 23 -Thr 24 -Phe 25 -Thr 26 -Ser 27 -Cys 28 (SEQ ID NO: 1)
  • A60 Glycosylated polypeptide in which dicialoglycosylated Cys is further added to the N-terminal side of Ala at position 15 (Example 17) (SEQ ID NO: 35);
  • A61 Glycosylated polypeptide in which dicialoglycosylated Cys-Arg-Lys- is further added to the N-terminal side of Ala at position 15 (Example 18) (SEQ ID NO: 36);
  • the glycosylated polypeptide of the present invention can be produced by incorporating a glycosylation step into a peptide synthesis method known to those skilled in the art.
  • a method using an enzyme represented by transglutaminase can also be used.
  • a large amount of sugar chain to be added is required, and purification after the final step becomes complicated.
  • problems such as restrictions on the position of the chain and the sugar chain that can be added, it can be used for small-scale synthesis for assays and the like, but it is practical for large-scale production such as pharmaceutical production. It may not be a method.
  • Method B A method in which the prepared peptide is produced according to a known peptide synthesis method and then a sugar chain is added to Cys by chemical synthesis to produce a glycosylated polypeptide.
  • these A method and B method can be performed in combination of two or more.
  • a sugar chain elongation reaction by a transferase with the above method.
  • the method A is described in International Publication No. 2004/005330 pamphlet (US2005222382 (A1))
  • the method B is described in International Publication No. 2005/010053 pamphlet (US2007060543 (A1)).
  • International Publication No. 03/008431 pamphlet US2004181054 (A1)
  • 2004/058884 pamphlet (US2006228784 (A1)). )), International Publication No. 2004/058824 pamphlet (US2006009421 (A1)), International Publication No. 2004/070046 pamphlet (US200620039 (A1)), International Publication No. 2007/011055 pamphlet, and the like. are incorporated herein by reference in their entirety.
  • a glycosylated polypeptide can be produced, for example, by solid phase synthesis using a glycosylated Asn as outlined below.
  • a glycosylated Asn as outlined below.
  • the carboxy group of an amino acid whose amino group nitrogen is protected with a fat-soluble protecting group is bonded to a resin (resin).
  • a resin resin
  • the amino group nitrogen of the amino acid is protected with a fat-soluble protecting group, self-condensation between the amino acids is prevented, and the resin and the amino acid react to form a bond.
  • the fat-soluble protecting group of the obtained reaction product is eliminated to form a free amino group.
  • a sugar chain-added Asn in which the amino group nitrogen is protected with a fat-soluble protecting group is used, and the carboxy group of the asparagine moiety is used. Can be obtained by reacting the hydroxyl group of the resin with the hydroxyl group of the resin.
  • a sugar chain can be added to any position by using sugar chain addition Asn in which the amino group nitrogen is protected with a fat-soluble protecting group.
  • a sugar in which the amino group nitrogen is protected with a fat-soluble protecting group instead of an amino acid in which the amino group nitrogen is protected with a fat-soluble protecting group, at least twice If chain-added Asn is used, a peptide having sugar chains added at two or more arbitrary positions can be obtained.
  • the fat-soluble protecting group is removed to form a free amino group, and immediately after that, step (6) is performed to obtain a peptide having a glycosylated Asn at the N-terminus. it can.
  • the resin (resin) is usually a resin (resin) used in solid-phase synthesis, such as 2-chlorotrityl chloride resin functionalized with chlorine (Merck) or functionalized with an amino group.
  • Amino-PEGA resin manufactured by Merck
  • NovaSyn TGT alcohol resin having a hydroxyl group manufactured by Merck
  • Wang resin manufactured by Merck
  • HMPA-PEGA resin manufactured by Merck
  • a linker may be present between the amino-PEGA resin and the amino acid. Examples of such a linker include 4-hydroxymethylphenoxyacetic acid (HMPA), 4- (4-hydroxymethyl-3-methoxyphenoxy).
  • H-Cys (Trt) -Trity NovaPEG resin manufactured by Merck or the like in which the C-terminal amino acid is bonded to the resin in advance can also be used.
  • a Rink-Amide-PEGA resin Merck
  • the C-terminal amino acid of the peptide can be amidated.
  • the bond between the resin and the amino acid whose amino group nitrogen is protected with a fat-soluble protecting group is obtained by esterifying the carboxy group of the amino acid to the resin.
  • the carboxy group of the amino acid is bonded to the resin by an amide bond.
  • 2-chlorotrityl chloride resin is preferable in that it can prevent racemization of Cys at the terminal when the peptide chain is extended in solid phase synthesis.
  • amino acids all amino acids can be used.
  • natural amino acids such as serine (Ser), asparagine (Asn), valine (Val), leucine (Leu), isoleucine (Ile), alanine (Ala), tyrosine (Tyr), glycine (Gly), lysine (Lys), arginine (Arg), histidine (His), aspartic acid (Asp), glutamic acid (Glu), glutamine (Gln), threonine (Thr), cysteine (Cys), Examples include methionine (Met), phenylalanine (Phe), tryptophan (Trp), and proline (Pro).
  • the D form of the natural amino acid can also be used.
  • the fat-soluble protective group examples include carbonate-based or amide groups such as 9-fluorenylmethoxycarbonyl (Fmoc) group, t-butyloxycarbonyl (Boc) group, benzyl group, allyl group, allyloxycarbonyl group, and acetyl group.
  • Protecting groups of the system can be mentioned.
  • a fat-soluble protecting group into an amino acid, for example, when introducing an Fmoc group, it can be introduced by adding 9-fluorenylmethyl-N-succinimidyl carbonate and sodium hydrogen carbonate to carry out the reaction. The reaction is carried out at 0 to 50 ° C., preferably at room temperature, for about 1 to 5 hours.
  • amino acids protected with a fat-soluble protecting group can also be used as amino acids protected with a fat-soluble protecting group.
  • amino acids protected with a fat-soluble protecting group and having a protecting group introduced in the side chain include, for example, Fmoc-Arg (Pbf) -OH, Fmoc-Asn (Trt) -OH, Fmoc-Asp (OtBu ) -OH, Fmoc-Cys (Acm) -OH, Fmoc-Cys (StBu) -OH, Fmoc-Cys (tBu) -OH, Fmoc-Cys (Trt) -OH, Fmoc-Glu (OtBu) -OH, Fmoc -Gln (Trt) -OH, Fmoc-His (Trt) -OH, Fmoc-Lys (Boc) -OH, Fmoc-Ser (tBu) -OH, Fmoc-Thr (tBu) -OH, Fmoc-Trp (Boc) -
  • esterification can be carried out by using a base such as diisopropylethylamine (DIPEA), triethylamine, pyridine, 2,4,6-collidine and the like.
  • DIPEA diisopropylethylamine
  • known esterification catalysts such as 1-mesitylenesulfonyl-3-nitro-1,2,4-triazole (MSNT), dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIC), etc.
  • MSNT 1-mesitylenesulfonyl-3-nitro-1,2,4-triazole
  • DCC dicyclohexylcarbodiimide
  • DIC diisopropylcarbodiimide
  • the dehydration condensing agent can be used.
  • the use ratio of the amino acid and the dehydration condensing agent is usually 1 to 10 parts by weight, preferably 2 to 5 parts by weight, with respect to 1 part by weight of the former.
  • the esterification reaction is preferably performed, for example, by placing a resin in a solid phase column, washing the resin with a solvent, and then adding an amino acid solution.
  • the cleaning solvent include dimethylformamide (DMF), 2-propanol, dichloromethane, and the like.
  • solvents that dissolve amino acids include dimethyl sulfoxide (DMSO), DMF, dichloromethane, and the like.
  • the esterification reaction is carried out at 0 to 50 ° C., preferably at room temperature, for about 10 minutes to 30 hours, preferably about 15 minutes to 24 hours.
  • the elimination of the lipophilic protecting group can be carried out, for example, by treatment with a base.
  • a base include piperidine and morpholine.
  • a solvent examples include DMSO, DMF, methanol and the like.
  • amidation reaction between the free amino group and the carboxy group of any amino acid whose amino group nitrogen is protected with a fat-soluble protecting group is preferably carried out in the presence of an activator and a solvent.
  • activator examples include dicyclohexylcarbodiimide (DCC), 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (WSC / HCl), diphenylphosphoryl azide (DPPA), carbonyldiimidazole (CDI).
  • DCC dicyclohexylcarbodiimide
  • WSC / HCl 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
  • DPPA diphenylphosphoryl azide
  • CDI carbonyldiimidazole
  • Diethyl cyanophosphonate (DEPC), benzotriazol-1-yloxy-trispyrrolidinophosphonium (DIPCI), benzotriazol-1-yloxy-trispyrrolidinophosphonium hexafluorophosphate (PyBOP), 1-hydroxybenzotriazole (HOBt), Hydroxysuccinimide (HOSu), dimethylaminopyridine (DMAP), 1-hydroxy-7-azabenzotriazole (HOAt), hydroxyphthalimide (HOP) t), pentafluorophenol (Pfp-OH), 2- (1H-benzotriazol-1-yl) -1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), 1- [bis (dimethyl Amino) methylene] -5-chloro-1H-benzotriazolium 3-oxide hexafluorophosphate (HCTU), O- (7-azabenzotriazol-1-yl)
  • the activator is used in an amount of 1 to 20 equivalents, preferably 1 to 10 equivalents, more preferably 1 to 5 equivalents with respect to any amino acid in which the amino group nitrogen is protected with a fat-soluble protecting group. Is preferred.
  • Examples of the solvent include DMSO, DMF, and dichloromethane.
  • the reaction is carried out at 0 to 50 ° C., preferably at room temperature, for about 10 to 30 hours, preferably about 15 minutes to 24 hours.
  • the elimination of the lipophilic protecting group can be carried out in the same manner as described above.
  • the acid include trifluoroacetic acid (TFA) and hydrogen fluoride (HF).
  • glycosylated polypeptide in which a desired position is substituted with glycosylated Asn can be obtained.
  • the glycosylated polypeptide thus purified forms a disulfide bond between deprotected Cys as described later.
  • the carboxy group of the sialic acid is protected by a protecting group.
  • the protecting group include benzyl group, allyl group, diphenylmethyl group and the like.
  • the introduction of the protecting group and the removal of the protecting group can be performed by known methods.
  • the protecting group may be removed before the disulfide bond forming step. It may be after the disulfide bond formation step.
  • the glycosylated polypeptide can also be produced by a method of first synthesizing a peptide chain and then adding the sugar chain to the synthesized peptide chain.
  • a peptide containing Cys at the position where a sugar chain is to be added is produced by a solid phase synthesis method, a liquid phase synthesis method, a method of synthesizing with a cell, a method of separating and extracting naturally occurring ones, and the like.
  • Cys that does not add a sugar chain such as Cys at a position where a disulfide bond is to be formed, is protected with, for example, an acetamidomethyl (Acm) group.
  • Cys that does not add a sugar chain and is not used for disulfide bond formation is introduced into a glycosylated polypeptide
  • Cys is protected by a protecting group during the sugar chain addition step and the disulfide bond formation step. Cys can be introduced so that it is protected and then deprotected. Examples of such a protecting group include tert-butyl (tBu) and 4-methoxybenzyl.
  • a protecting group include tert-butyl (tBu) and 4-methoxybenzyl.
  • the Cys to which the first sugar chain is to be introduced is unprotected and the Cys to which the second sugar chain is to be introduced is Fmoc-Cys (StBu) -OH. And so on, to obtain Cys having a protecting group. Thereafter, a sugar chain is introduced into unprotected Cys while retaining a protecting group such as StBu. Next, by deprotecting the StBu group and the like, different sugar chains can be introduced into the unprotected Cys. One or a plurality of Cys into which the first sugar chain is to be introduced and Cys into which the second sugar chain is to be introduced can be used.
  • the StBu group can be deprotected by reacting with a reducing agent such as tris (2-carboxyethyl) phosphine hydrochloride (TCEP), dithiothreitol (DTT), or tributylphosphine.
  • TCEP tris (2-carboxyethyl) phosphine hydrochloride
  • DTT dithiothreitol
  • tributylphosphine tributylphosphine.
  • the above reaction is usually carried out at 0 to 80 ° C., preferably 5 to 60 ° C., more preferably 10 to 35 ° C.
  • the reaction time is preferably about 30 minutes to 5 hours. After completion of the reaction, it may be appropriately purified by a known method (for example, high performance liquid column chromatography (HPLC)).
  • HPLC high performance liquid column chromatography
  • a sugar chain that is more stable with respect to the reducing conditions in the Cys deprotection step and the acidic conditions in the purification step such as HPLC it is preferable to introduce a sugar chain that is more stable with respect to the reducing conditions in the Cys deprotection step and the acidic conditions in the purification step such as HPLC.
  • a sialic acid-containing sugar chain it is preferable to introduce a sugar chain that does not have sialic acid or a sugar chain having a small number of sialic acid residues first.
  • a linker is to be added to the amino acid sequence of a glycosylated polypeptide, for example, in the process of solid phase synthesis, a linker protected with a fat-soluble protecting group instead of an amino acid protected with a fat-soluble protecting group.
  • the sugar chain is reacted with the thiol group of unprotected Cys and bound to the peptide.
  • the above reaction is usually performed at 0 to 80 ° C., preferably 10 to 60 ° C., more preferably 15 to 35 ° C. in a phosphate buffer, Tris-HCl buffer, citrate buffer, or a mixed solution thereof. Good to do.
  • the reaction time is usually about 10 minutes to 24 hours, preferably about 30 minutes to 5 hours. After completion of the reaction, it may be appropriately purified by a known method (for example, HPLC).
  • the haloacetylated complex-type sugar chain derivative for example, represents a hydroxyl group bonded to the 1-position carbon of a complex-type asparagine-linked sugar chain by —NH— (CH 2 ) a — (CO) —CH 2 X (X is The halogen atom, a is an integer, and is not limited as long as the desired linker function is not inhibited, but preferably represents an integer of 0 to 4.
  • a haloacetylated complex type sugar chain derivative and a Cys-containing peptide are reacted in a phosphate buffer at room temperature.
  • a glycosylated polypeptide substituted with glycosylated Cys can be obtained by purification with HPLC.
  • the reaction can also be performed in a mixed solution of an organic solvent such as DMSO, DMF, methanol, and acetonitrile and the above buffer solution. At this time, the ratio of the organic solvent can be added to the buffer solution in the range of 0 to 99% (v / v).
  • a peptide containing unprotected Cys having low solubility in a buffer solution is preferable because it can improve solubility in a reaction solution by adding such an organic solvent.
  • the reaction can also be carried out in an organic solvent such as DMSO, DMF, methanol, acetonitrile, or a mixed solution thereof. In that case, it is preferable to carry out in the presence of a base.
  • the base include DIPEA, triethylamine, pyridine, 2,4,6-collidine and the like.
  • the reaction can also be performed in a mixed solution in which guanidine hydrochloride or urea is added to the buffer solution.
  • Guanidine hydrochloride and urea can be added to the buffer so that the final concentration is 1M to 8M. Addition of guanidine hydrochloride or urea is preferable because it can improve the solubility of a peptide having low solubility in a buffer solution. Furthermore, in order to prevent peptides containing unprotected Cys from forming dimers via disulfide bonds, tris (2-carboxyethyl) phosphine hydrochloride (TCEP) and dithiothreitol (DTT) are used. It can also be made to react by adding to a buffer solution. TCEP and DTT can be added to the buffer so that the final concentration is 10 ⁇ M to 10 mM.
  • TCEP (2-carboxyethyl) phosphine hydrochloride
  • DTT dithiothreitol
  • the Cys protecting group protected with Acm or the like is deprotected.
  • the protecting group is an Acm group
  • the reaction is performed using iodine, mercury (II) acetate, silver nitrate (I), silver (I) acetate, or the like in water, methanol, acetic acid, or a mixed solution thereof. Can be deprotected.
  • the above reaction is usually carried out at 0 to 80 ° C., preferably 5 to 60 ° C., more preferably 10 to 35 ° C.
  • the reaction time is preferably about 5 minutes to 24 hours.
  • glycosylated polypeptide After completion of the reaction, after treatment with DTT, hydrochloric acid or the like, it may be appropriately purified by a known method (for example, HPLC). In this manner, a glycosylated polypeptide in which a desired position is substituted with glycosylated Cys can be obtained. Moreover, the glycosylated polypeptide thus purified forms a disulfide bond between deprotected Cys as described later.
  • the glycosylated polypeptide prepared by the above methods A and B is a mixture of air and / or oxygen, iodine, DMSO, oxidized and reduced glutathione, potassium ferricyanate, Elman reagent (5,5 ′
  • a disulfide bond between Cys can be formed by a method well known to those skilled in the art using dithiobis (2-nitrobenzoic acid)), thallium trifluoroacetate (III), alkyltrichlorosilane sulfoxide, and the like.
  • Cys in a glycosylated polypeptide for which disulfide bond is not desirable is protected with a protecting group.
  • a protecting group which is stable under oxidizing conditions such as Acm, tBu, 4-methoxybenzyl, 4-methylbenzyl and the like can be used.
  • Method B disulfide bonds can be formed before introduction of sugar chains.
  • the deprotection step precedes the disulfide bond formation step.
  • the glycosylated polypeptide of the present invention has an affinity for somatostatin receptors.
  • affinity for somatostatin receptor means having affinity for at least one of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 which are somatostatin receptors.
  • the glycosylated polypeptide of the present invention preferably has affinity for two or more somatostatin receptors, more preferably has affinity for three or more receptors, and more preferably four or more. And most preferably has affinity for all five receptors SSTR1 to SSTR5, similar to natural somatostatin (SRIF28 and SRIF14). In particular, it is preferable to have affinity for at least one of SSTR1 and SSTR4 and affinity for other SSTRs.
  • the glycosylated polypeptide of the present invention has somatostatin activity (agonist activity) and antagonist activity for the somatostatin receptor by having affinity for the somatostatin receptor.
  • the affinity for each somatostatin receptor can be measured using in vitro competitive binding experiments as shown in Examples 67-1 and 67-2.
  • Affinity measurement by competitive binding experiments is performed by competitively binding a labeled ligand and a test substance to a receptor, and determining the release of the labeled ligand by administration of the test substance. 50% inhibition concentration: IC 50 value and binding inhibition constant: Ki value) can be measured.
  • somatostatin activity can be evaluated by an in vitro cAMP production inhibition test using somatostatin receptor-expressing cells as shown in Examples 67-3 and 67-4.
  • cAMP production inhibition test somatostatin receptor-expressing cells are treated with a glycosylated polypeptide or a control compound SRIF14 or SRIF28, and after culturing for a certain period of time, the amount of cAMP accumulated in the cells is measured. It can be evaluated by comparing with. The amount of cAMP can be measured by a known method such as enzyme immunoassay (EIA).
  • EIA enzyme immunoassay
  • somatostatin activity (agonist activity) can be evaluated by an in vivo GH production inhibition test as shown in Example 86 and Example 87.
  • the GH production suppression test can be performed, for example, as follows. Glycosylated polypeptides are administered subcutaneously to non-fasted rats. Rats are administered a GH release promoter under general anesthesia, and blood is collected after about 5 minutes. Using the collected blood as a plasma sample, the GH concentration is measured by a known method such as enzyme immunoassay (EIA). As a control, somatostatin activity can be evaluated by obtaining a plasma sample from a rat administered with physiological saline containing no glycosylated polypeptide, by comparing the measured GH concentrations.
  • EIA enzyme immunoassay
  • the glycosylated polypeptide of the present invention has a prolonged half-life in blood even if the addition of a sugar chain to the structure causes a certain amount of affinity decrease for each receptor. It can maintain somatostatin activity equivalent to that of naturally occurring somatostatin to which no sugar chain is added (hereinafter sometimes referred to as “non-glycosylated somatostatin”), and preferably has increased somatostatin activity. Can do.
  • the Ki value for each receptor of the glycosylated polypeptide of the present invention and the sugar chain is preferably in the range of 1000: 1 to 0.3: 1, more preferably in the range of 100: 1 to 0.3: 1. More preferably, it is within the range of 10: 1 to 0.3: 1.
  • the blood stability of the glycosylated polypeptide of the present invention is preferably equal to or higher than that of naturally occurring somatostatin (non-glycosylated somatostatin).
  • Blood stability can be measured using methods known to those skilled in the art. For example, the stability in plasma, blood half-life, AUC (area under the drug plasma concentration-time curve), etc. are used as indicators. Judgment can be made. In addition, a decrease in renal clearance and liver clearance also contributes to an increase in blood stability.
  • the glycosylated polypeptide of the present invention has an increased blood half-life compared to SRIF28 having no sugar chain.
  • the half-life is: Compared with SRIF28, it is increased 4 times or more, preferably 10 times or more, more preferably 20 times or more, and further preferably 30 times or more.
  • glycosylated polypeptide of the present invention is preferably 4 times or more, more preferably 10 times or more, still more preferably 20 times or more, as compared with SRIF28, for example, when measured by the experimental method shown in Example 85. Has blood stability.
  • composition a pharmaceutical composition containing the glycosylated polypeptide of the present invention as an active ingredient
  • the pharmaceutical composition containing the glycosylated polypeptide of the present invention as an active ingredient is effective for the treatment or prevention of diseases associated with somatostatin.
  • various actions are known for somatostatin, and there are various diseases associated with these actions.
  • diseases associated with somatostatin include, for example, acromegaly, giantism, Alzheimer's disease and other forms of dementia, cancer, hormone producing tumors, endocrine tumors (eg carcinoids, VIPomas, insulinomas, glucagonomas), cushing Disease, hormonal secretion, diabetes and its complications, pain, arthritis, diarrhea, gastric ulcer, inflammatory bowel disease, irritable bowel syndrome, gastrointestinal obstruction, ileus, postoperative restenosis, radiation damage, eye disease (eg dry eye , Glaucoma, inflammation of the corneal stroma, ulceris, cataract, conjunctivitis) and the like.
  • endocrine tumors eg carcinoids, VIPomas, insulinomas, glucagonomas
  • cushing Disease hormonal secretion
  • diabetes and its complications pain, arthritis, diarrhea, gastric ulcer, inflammatory bowel disease, irritable bowel syndrome, gastrointestinal obstruction, ileus, postoperative restenosis, radiation damage, eye disease (eg dry
  • the pharmaceutical composition containing the glycosylated polypeptide of the present invention as an active ingredient is the above-mentioned diseases, particularly acromegaly, dementia, cancer, hormone-producing tumor, endocrine tumor, Cushing disease, hormone secretion abnormality, diabetic complication, It is effective for the treatment or prevention of diarrhea, gastrointestinal obstruction, ileus, radiation disorders, eye diseases, as well as various tumors or intestinal related diseases, and gastrointestinal symptoms associated with excessive hormone production.
  • the above pharmaceutical composition is a normal pharmaceutical composition using diluents or excipients such as fillers, extenders, binders, moisturizers, disintegrants, surfactants, lubricants and the like that are usually used. It was formulated in the form of Examples of such a pharmaceutical composition include tablets, pills, powders, solutions, suspensions, emulsions, granules, capsules, suppositories, inhalants, eye drops, injections, and the like.
  • diluents or excipients such as fillers, extenders, binders, moisturizers, disintegrants, surfactants, lubricants and the like that are usually used. It was formulated in the form of Examples of such a pharmaceutical composition include tablets, pills, powders, solutions, suspensions, emulsions, granules, capsules, suppositories, inhalants, eye drops, injections, and the like.
  • the amount of the glycosylated polypeptide of the present invention contained in the pharmaceutical composition is not particularly limited and can be appropriately selected from a wide range. Usually, the glycosylated polypeptide of the present invention is contained in the pharmaceutical composition. It is preferable to contain 1 to 70% by weight of the peptide.
  • the pharmaceutical composition containing the glycosylated polypeptide of the present invention as an active ingredient can further contain other active ingredients, or can be used in combination with a pharmaceutical composition containing other active ingredients.
  • the pharmaceutical composition containing the glycosylated polypeptide of the present invention as an active ingredient may contain the glycosylated polypeptide as a pharmaceutically acceptable salt, or may be one or more different sugars of the present invention.
  • a chain-added polypeptide can also be contained as an active ingredient.
  • the treatment using the glycosylated polypeptide of the present invention can include, for example, radiotherapy, and for measuring the distribution of cells and tissues expressing any of SSTR1 to SSTR5 throughout the body. It is also useful in scintigraphy.
  • the use of in vitro imaging with radioactive scanning or magnetic resonance can allow for semi-quantitative detection in the body.
  • Radiolabeled glycosylated polypeptides are useful for therapeutic treatment of malignant tumors expressing any of SSTR1 to SSTR5 in, for example, the human body, in tissues that do not normally contain substantial amounts of SSTR1 to SSTR5. Useful for.
  • the labeled glycosylated polypeptide can be administered as a composition containing an effective amount for scintigraphy or for tumor suppression. Examples of such labels include iodine (123 I, 125 I, 131 I), indium (111 an In), carbon (11 C), fluorine (18 F), technetium (99 mTc), yttrium (90 Y), etc. Different isotopes, or fluorescent labels.
  • Labeling can be performed by methods well known to those skilled in the art and are included in the glycosylated polypeptide, directly linked, or conjugated to an appropriate compound, which is linked to the glycosylated polypeptide. Can be combined. For example, tyrosine iodination can be labeled by a method using chloramine-T or the like.
  • the administration method of the pharmaceutical composition according to the present invention is not particularly limited, and it is administered by a method according to various preparation forms, patient age, sex, disease state, and other conditions.
  • Examples of the administration method in the case of tablets, pills, solutions, suspensions, emulsions, granules and capsules include oral administration.
  • it can be administered intravenously, intramuscularly, intradermally, subcutaneously or intraperitoneally alone or mixed with a normal fluid such as glucose or amino acid.
  • a suppository it is administered intrarectally.
  • eye drops apply to eye tissues such as conjunctival sac.
  • For inhalants apply to bronchi or lungs.
  • the dosage of the above pharmaceutical composition may be appropriately selected according to usage, patient age, sex, disease severity, and other conditions.
  • the glycosylated polypeptide of the present invention is 0 per 1 kg body weight.
  • the dosage may be 1 to 900 nmol, preferably 1 to 100 nmol, more preferably 1 to 10 nmol.
  • the number of administrations of the pharmaceutical composition may be appropriately selected according to usage, patient age, sex, disease severity, and other conditions. For example, 3 times / 1 day, 2 times / 1 day, 1 time / A less frequent number of administrations (for example, once / week, once / month, etc.) may be selected depending on the stability of the blood in one day.
  • the frequency of administration of the pharmaceutical composition is not more than once / day.
  • the sugar chain added to the glycosylated polypeptide of the present invention is easily degraded in the body's metabolic system.
  • the sugar chain has a structure that exists as a glycopeptide (or glycoprotein) in vivo. Therefore, the glycosylated polypeptide of the present invention and the pharmaceutical composition containing the peptide as an active ingredient do not show side effects or antigenicity even when administered in vivo, and have a medicinal effect by allergic reaction or antibody production. It has advantages such as less worry about being lost.
  • the glycosylated polypeptide of the present invention can be stably and easily supplied in large quantities, and is very useful from the viewpoint of providing a high-quality pharmaceutical product with stable quality.
  • the present invention also provides a method for treating or preventing a disease associated with somatostatin, which comprises administering an effective amount of the glycosylated polypeptide of the present invention.
  • Embodiments of the present invention may be described with reference to schematic diagrams, but in the case of schematic diagrams, they may be exaggerated for clarity of explanation.
  • terms such as first, second, etc. are used to represent various elements, it is understood that these elements should not be limited by those terms. These terms are only used to distinguish one element from another, for example, the first element is referred to as the second element, and similarly, the second element is the first element. Can be made without departing from the scope of the present invention.
  • Trp at position 22 is substituted with D-Trp.
  • C (disialo) -RK-SRIF14 indicates that a glycosylated Cys-Arg-Lys- was added to the N-terminal side of SRIF14.
  • S1-2C (disialo) -SRIF28 indicates that the Ser at position 1 present at the N-terminal of the polypeptide of SRIF28 is replaced with two consecutive dicialoglycosylated Cys. .
  • “Disialo” means a dicialo sugar chain
  • “monosialo” means a monosialo glycan
  • “asialo” means an asialog glycan
  • “diGlcNAc” means a diglucnac glycan
  • “GlcNAc” means N-acetylglucosamine means
  • diMan means dimannose sugar chain
  • trisialo means trisialo sugar chain
  • tetrasialo means tetrasialo sugar chain.
  • aminoethylamide In the case of (disialo (aminoethylamide)), it means that the carboxy group of the sialic acid of the dicialo sugar chain is protected by an aminoethylamino group.
  • aminoethylamide those indicated as “Bn”, “amide”, and “hexadecylamide” are modified with the carboxy group of sialic acid on the sugar chain by benzyl group, amino group, hexadecylamino group, respectively.
  • Example 2 Synthesis of N5C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound (peptide 5) (SEQ ID NO: 41) represented by the following formula (5) was used instead of peptide 1, A compound represented by the formula (6) (N5C (disialo) -SRIF28) (SEQ ID NO: 6) was synthesized.
  • Example 3 Synthesis of A9C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (7) (peptide 7) (SEQ ID NO: 42) was used instead of peptide 1, A compound represented by the formula (8) (A9C (disialo) -SRIF28) (SEQ ID NO: 7) was synthesized.
  • Example 4 Synthesis of E12C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound (peptide 9) (SEQ ID NO: 43) represented by the following formula (9) was used instead of peptide 1, A compound represented by the formula (10) (E12C (disialo) -SRIF28) (SEQ ID NO: 8) was synthesized.
  • Example 5 Synthesis of R13C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (11) (peptide 11) (SEQ ID NO: 44) was used instead of peptide 1, A compound represented by formula (12) (R13C (disialo) -SRIF28) (SEQ ID NO: 9) was synthesized.
  • Example 6 Synthesis of K14C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (13) (peptide 13) (SEQ ID NO: 45) was used instead of peptide 1, A compound represented by formula (14) (K14C (disialo) -SRIF28) (SEQ ID NO: 10) was synthesized.
  • Example 7 Synthesis of A15C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (15) (peptide 15) (SEQ ID NO: 46) was used instead of peptide 1, A compound represented by formula (16) (A15C (disialo) -SRIF28) (SEQ ID NO: 11) was synthesized.
  • Example 8 Synthesis of G16C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (17) (peptide 17) (SEQ ID NO: 47) was used instead of peptide 1, A compound represented by the formula (18) (G16C (disialo) -SRIF28) (SEQ ID NO: 12) was synthesized.
  • Example 9 Synthesis of K18C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (19) (peptide 19) (SEQ ID NO: 48) was used instead of peptide 1, A compound represented by the formula (20) (K18C (disialo) -SRIF28) (SEQ ID NO: 13) was synthesized.
  • Example 10 Synthesis of N19C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound (peptide 21) (SEQ ID NO: 49) represented by the following formula (21) was used instead of peptide 1, the following: A compound represented by formula (22) (N19C (disialo) -SRIF28) (SEQ ID NO: 14) was synthesized.
  • Example 11 Synthesis of F21C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (23) (peptide 23) (SEQ ID NO: 50) was used instead of peptide 1, A compound represented by the formula (24) (F21C (disialo) -SRIF28) (SEQ ID NO: 15) was synthesized.
  • Example 12 Synthesis of T26C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (25) (peptide 25) (SEQ ID NO: 51) was used instead of peptide 1, A compound represented by the formula (26) (T26C (disialo) -SRIF28) (SEQ ID NO: 16) was synthesized.
  • Example 13 Synthesis of 29C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound (peptide 27) (SEQ ID NO: 52) represented by the following formula (27) was used instead of peptide 1, A compound represented by the formula (28) (29C (disialo) -SRIF28) (SEQ ID NO: 17) was synthesized.
  • Example 14 Synthesis of 30C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (29) (peptide 29) (SEQ ID NO: 53) was used instead of peptide 1, A compound represented by the formula (30) (30C (disialo) -SRIF28) (SEQ ID NO: 18) was synthesized.
  • Example 15 Synthesis of S1C (disialo) -D-Trp22-SRIF28 Same as Example 1 except that instead of peptide 1, a compound represented by the following formula (31) (peptide 31) (SEQ ID NO: 54) was used Thus, a compound represented by the following formula (32) (S1C (disialo) -D-Trp22-SRIF28) (SEQ ID NO: 19) was synthesized.
  • Example 16 Synthesis of A9C (disialo) -D-Trp22-SRIF28 Same as Example 1 except that the compound represented by the following formula (33) (peptide 33) (SEQ ID NO: 55) was used instead of peptide 1. Then, a compound (A9C (disialo) -D-Trp22-SRIF28) (SEQ ID NO: 20) represented by the following formula (34) was synthesized.
  • Example 17 Synthesis of C (disialo) -SRIF14 In the same manner as in Example 1 except that the compound represented by the following formula (35) (peptide 35) (SEQ ID NO: 56) was used instead of peptide 1, A compound represented by formula (36) (C (disialo) -SRIF14) (SEQ ID NO: 35) was synthesized.
  • Example 18 Synthesis of C (disialo) -RK-SRIF14 Similar to Example 1 except that the compound represented by the following formula (37) (peptide 37) (SEQ ID NO: 57) was used instead of peptide 1. Thus, a compound (C (disialo) -RK-SRIF14) (SEQ ID NO: 36) represented by the following formula (38) was synthesized.
  • Example 19 Synthesis of C (disialo) -C12linker-SRIF14 19-1 Synthesis of Peptide 2-chlorotrityl chloride resin (100 ⁇ mol) was taken in a solid phase synthesis column, washed with DMF and dichloromethane, and then Fmoc-Cys (Acm). A solution of —OH (49.7 mg, 120 ⁇ mol) and DIPEA (104.5 ⁇ L, 600 ⁇ mol) in dichloromethane (3.0 mL) was added and shaken for 1 hour. After washing with dichloromethane and DMF, the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • a protected peptide 39 represented by the following formula (39), which is represented by the following formula (39), in a peptide solid phase synthesis method by Fmoc method using a Prelude (trademark) peptide synthesizer No. 58) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • Fmoc-12-aminododecanoic acid and Fmoc-Cys (Trt) -OH were sequentially condensed using HCTU as a condensing agent.
  • 19-2 Sugar chain modification reaction of thiol Peptide 40 (6.8 mg, 3.3 ⁇ mol) obtained by the method described in 19-1 above and compound a (19.1 mg, 8. 15 ⁇ mol) was dissolved in 0.2 M phosphate buffer (pH 7.4, 0.96 mL) containing 7 M guanidine hydrochloride and 330 ⁇ M TCEP, and reacted at room temperature for 2 hours.
  • the protected peptide was synthesized on the resin by the peptide solid phase synthesis method by the Fmoc method using a Prelude (trademark) peptide synthesizer.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the resin was removed by filtration, and cooled diethyl ether was added to the filtrate to obtain a crude peptide as a precipitate.
  • Example 21 Synthesis of S1C (disialo) ⁇ N5C (disialo) -SRIF28
  • Example 20 was used except that a compound represented by the following formula (48) (peptide 48) (SEQ ID NO: 65) was used instead of peptide 44.
  • a compound represented by the following formula (49) (S1C (disialo) ⁇ N5C (disialo) -SRIF28) (SEQ ID NO: 22) was synthesized.
  • Example 22 Synthesis of S1C (disialo) / R13C (disialo) -SRIF28
  • Example 20 was used except that a compound represented by the following formula (50) (peptide 50) (SEQ ID NO: 66) was used instead of peptide 44.
  • a compound represented by the following formula (51) (S1C (disialo) ⁇ R13C (disialo) -SRIF28) (SEQ ID NO: 23) was synthesized.
  • Example 23 Synthesis of N5C (disialo) / A9C (disialo) -SRIF28
  • Example 20 Similarly, a compound represented by the following formula (53) (N5C (disialo) / A9C (disialo) -SRIF28) (SEQ ID NO: 24) was synthesized.
  • a protected peptide was synthesized on a resin by a peptide solid phase synthesis method by Fmoc method using a Prelude (trademark) peptide synthesizer.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the resin was removed by filtration, and cooled diethyl ether was added to the filtrate to obtain a crude peptide as a precipitate.
  • Example 25 Synthesis of S1C (disialo), N5C (disialo), A9C (disialo) -SRIF28 Example except that peptide 58 (SEQ ID NO: 71) represented by the following formula (58) was used instead of peptide 54 In the same manner as in 24, a compound represented by the following formula (59) (S1C (disialo) ⁇ N5C (disialo) ⁇ A9C (disialo) -SRIF28) (SEQ ID NO: 26) was synthesized.
  • Example 26 Synthesis of S1C (monosialo) -SRIF28 Example 1 except that compound b represented by the following formula (b) (bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.) was used instead of compound a.
  • compound b represented by the following formula (b) bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.
  • a compound represented by the following formula (60) S1C (monosialo) -SRIF28) (SEQ ID NO: 27) was synthesized.
  • the ratio of the sugar chain polypeptide having a sugar chain of the following formula b1 to the sugar chain-added polypeptide having a sugar chain of the following formula b2 was 45:55.
  • monosialo sugar chain derivatives having the same structure a glycosylated polypeptide having a substantially uniform sugar chain structure can be produced.
  • Example 27 Synthesis of S1C (asialo) -SRIF28 Example 1 except that compound c represented by the following formula (c) (bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.) was used instead of compound a.
  • compound c represented by the following formula (c) bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.
  • Example 30 Synthesis of N5N (disialo) -SRIF28 30-1 Solid Phase Synthesis of Glycopeptide 2-chlorotrityl chloride resin (100 ⁇ mol) was taken in a column for solid phase synthesis, washed with DMF and dichloromethane, and then Fmoc-Cys (Trt ) -OH (72.5 mg, 120 ⁇ mol) and DIPEA (104.6 ⁇ L, 600 ⁇ mol) in dichloromethane (3.0 mL) were added and shaken for 1 hour. After washing with dichloromethane and DMF, the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the protected peptide 68 (sequence) represented by the following formula (68), in a peptide solid phase synthesis method by Fmoc method, using a Prelude (trademark) peptide synthesizer. No. 76) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was then removed by treatment with 20% piperidine in DMF.
  • compound d represented by the following formula (d) (manufactured by Otsuka Chemical Co., Ltd.) (411.9 mg, 150.4 ⁇ mol), DMSO-DMF (1/1, v / v, 871 ⁇ L) solution, TBTU ( 64.2 mg, 200 ⁇ mol) and DIPEA (52.3 ⁇ L, 300 ⁇ mol) were sequentially added to the resin and condensed by shaking at room temperature for 3 hours. After washing with DMF, this condensation operation was repeated once.
  • formula (d) manufactured by Otsuka Chemical Co., Ltd.
  • the resin was washed with DMF and dichloromethane, then shaken with 20% piperidine in DMF for 20 minutes to remove the Fmoc group, and the resin was washed with DMF to protect the compound represented by the following formula (69) (peptide) 69) (SEQ ID NO: 77) was synthesized on the resin.
  • Fmoc-Ser (tBu) -OH, Fmoc-Asn (Trt) -OH, Fmoc-Ala, and Fmoc-Ser (tBu) -OH were sequentially condensed on this resin using HOBt / DIC as a condensing agent. After condensation, the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • glycopeptide 70 SEQ ID NO: 78 (29.1 mg, 5.26 ⁇ mol) represented by the following formula (70).
  • a protected peptide 73 (sequence) represented by the following formula (73) in a peptide solid phase synthesis method by the Fmoc method using a Prelude (trademark) peptide synthesizer and bound to a resin No. 80) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was then removed by treatment with 20% piperidine in DMF.
  • ESI-MS (m / z) calcd for C 236 H 356 N 48 O 100 S 3 : [M + 4H] 4+ 1391.5, [M + 5H] 5+ 1113.4, found 1391.3, 113.2.
  • the protected peptide was synthesized on the resin by the peptide solid phase synthesis method by the Fmoc method using a Prelude (trademark) peptide synthesizer.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the resin was removed by filtration, and cooled diethyl ether was added to the filtrate to obtain a crude peptide as a precipitate.
  • ESI-MS (m / z) calcd for C 152 H 234 N 44 O 45 S 5 [M + 3H] 3+ 1187.0, [M + 4H] 4+ 890.5, found 1187.0, 890.5.
  • Example 33 Synthesis of S1C (disialo) ⁇ N19C (diMan) -SRIF28 Except for using compound f (bromoacetamidized oligosaccharide: Otsuka Chemical Co., Ltd.) represented by the following formula (f) instead of compound e
  • f bromoacetamidized oligosaccharide: Otsuka Chemical Co., Ltd.
  • a compound represented by the following formula (83) S1C (disialo) ⁇ N19C (diMan) -SRIF28) (SEQ ID NO: 34) was synthesized.
  • Example 34 Synthesis of C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound (peptide 84) (SEQ ID NO: 88) represented by the following formula (84) was used instead of peptide 1, the following. A compound represented by the formula (85) (C (disialo) -SRIF28) (SEQ ID NO: 89) was synthesized.
  • Example 35 Synthesis of R11C (disialo) -SRIF28 The same procedure as in Example 1 was conducted except that the compound represented by the following formula (86) (peptide 86) (SEQ ID NO: 90) was used instead of peptide 1. A compound represented by the formula (87) (R11C (disialo) -SRIF28) (SEQ ID NO: 91) was synthesized.
  • Example 36 Synthesis of F20C (disialo) -SRIF28 The same procedure as in Example 1 was conducted except that the compound represented by the following formula (88) (peptide 88) (SEQ ID NO: 92) was used instead of peptide 1. A compound represented by the formula (89) (F20C (disialo) -SRIF28) (SEQ ID NO: 93) was synthesized.
  • Example 37 Synthesis of T24C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (90) (peptide 90) (SEQ ID NO: 94) was used instead of peptide 1, A compound represented by the formula (91) (T24C (disialo) -SRIF28) (SEQ ID NO: 95) was synthesized.
  • Example 38 Synthesis of F25C (disialo) -SRIF28 The same procedure as in Example 1 was conducted except that the compound represented by the following formula (92) (peptide 92) (SEQ ID NO: 96) was used instead of peptide 1. A compound represented by the formula (93) (F25C (disialo) -SRIF28) (SEQ ID NO: 97) was synthesized.
  • Example 39 Synthesis of S27C (disialo) -SRIF28 In the same manner as in Example 1 except that the compound represented by the following formula (94) (peptide 94) (SEQ ID NO: 98) was used instead of peptide 1, A compound represented by the formula (95) (S27C (disialo) -SRIF28) (SEQ ID NO: 99) was synthesized.
  • Example 40 Synthesis of C (disialo) -K-SRIF14 The same procedure as in Example 1 was conducted except that a compound represented by the following formula (96) (peptide 96) (SEQ ID NO: 100) was used instead of peptide 1. Then, a compound (C (disialo) -K-SRIF14) (SEQ ID NO: 101) represented by the following formula (97) was synthesized.
  • Example 41 Synthesis of S1C (disialo) -F25Y-SRIF28 The same procedure as in Example 1 was conducted, except that the compound represented by the following formula (98) (peptide 98) (SEQ ID NO: 102) was used instead of peptide 1. Then, a compound represented by the following formula (99) (S1C (disialo) -F25Y-SRIF28) (SEQ ID NO: 103) was synthesized.
  • Example 42 Synthesis of S1C (disialo) -SRIF28-amide In the same manner as in Example 1 except that the compound represented by the following formula (100) (peptide 100) (SEQ ID NO: 104) was used instead of peptide 1. Then, a compound represented by the following formula (101) (S1C (disialo) -SRIF28-amide) (SEQ ID NO: 105) was synthesized.
  • Example 43 Synthesis of C (disialo) -PEGlinker-SRIF14 43-1 Synthesis of peptide Protected peptide 39 (SEQ ID NO: 58) bound to the resin obtained by the method described in 19-1 above (50 ⁇ mol) ) was removed by treatment with 20% piperidine in DMF. After washing with DMF, Fmoc-NH- (PEG) 2 -COOH (manufactured by Merck) and Fmoc-Cys (Trt) -OH were sequentially condensed using HCTU as a condensing agent. The Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the resin was removed by filtration, and cooled diethyl ether was added to the filtrate to obtain a crude peptide as a precipitate.
  • the protected peptide 104 (sequence) in a state bound to the resin represented by the following formula (104) by the peptide solid phase synthesis method by Fmoc method using a Prelude (trademark) peptide synthesizer No. 108) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • biotin was condensed using HCTU as a condensing agent.
  • amino acid side chain protecting groups other than the Acm group
  • the resin was removed by filtration, and cooled diethyl ether was added to the filtrate to obtain a crude peptide as a precipitate.
  • Example 46 Synthesis of Azido-S1C (disalo) -SRIF28 46-1 Synthesis of peptide
  • Example 47 Synthesis of S1C (disialo) / E12C (disialo) -SRIF28 Except that peptide 44 was synthesized and used instead of peptide 44, the compound (peptide 111) (SEQ ID NO: 115) was used. In the same manner as in Example 20, a compound represented by the following formula (112) (S1C (disialo) ⁇ E12C (disialo) -SRIF28) (SEQ ID NO: 116) was synthesized.
  • Example 48 Synthesis of 2C (disialo) -RK-SRIF14 Example except that compound (peptide 113) (SEQ ID NO: 117) represented by the following formula (113) was synthesized and used instead of peptide 44 In the same manner as in Example 20, a compound (2C (disialo) -RK-SRIF14) (SEQ ID NO: 118) represented by the following formula (114) was synthesized.
  • Example 49 Synthesis of 3C (disialo) -RK-SRIF14
  • Example 3 except that compound (peptide 115) (SEQ ID NO: 119) represented by the following formula (115) was synthesized and used instead of peptide 54
  • Example 51 Synthesis of S1C (diMan) -SRIF28 A compound represented by the following formula (120) (S1C (diMan) -SRIF28) was obtained in the same manner as in Example 50 except that Compound f was used instead of Compound h. (SEQ ID NO: 124) was synthesized.
  • Example 52 Synthesis of N19C (diMan) -SRIF28 This was represented by the following formula (121) in the same manner as in Example 50 except that peptide 21 was used instead of peptide 1 and compound f was used instead of compound h. (N19C (diMan) -SRIF28) (SEQ ID NO: 125) was synthesized.
  • Example 53 Synthesis of S1C (GlcNAc) -SRIF28 A compound represented by the following formula (122) (S1C (GlcNAc) -SRIF28) was obtained in the same manner as in Example 50 except that compound e was used instead of compound h. (SEQ ID NO: 126) was synthesized.
  • Example 54 Synthesis of N19C (GlcNAc) -SRIF28 A compound represented by the following formula (123) was obtained in the same manner as in Example 50 except that peptide 21 was used instead of peptide 1 and compound e was used instead of compound h. (N19C (GlcNAc) -SRIF28) (SEQ ID NO: 127) was synthesized.
  • Example 55 Synthesis of S1C (trisialo) -SRIF28 Example 1 except that compound i represented by the following formula (i) (bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.) was used instead of compound a.
  • compound (S1C (trisialo) -SRIF28) (SEQ ID NO: 128) represented by the following formula (124) was synthesized. (I)
  • Example 56 Synthesis of S1C (tetrasia) -SRIF28 Example 1 except that compound j represented by the following formula (j) (bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.) was used instead of compound a.
  • compound j represented by the following formula (j) bromoacetamidized oligosaccharide: manufactured by Otsuka Chemical Co., Ltd.
  • 57-2 Sugar chain modification reaction of thiol Compound m (14.2 mg, 5.40 ⁇ mol) obtained by the method described in 57-1 and peptide 1 (15.0 mg, 4.53 ⁇ mol) were mixed with 33 mM phosphate buffer. (PH 7.4, 1.3 mL) was dissolved and reacted at room temperature for 30 minutes.
  • S1C (disialo (Bn))-SRIF28 A compound represented by the following formula (131) (S1C (disialo (S)) was prepared in the same manner as in Example 1 except that Compound g was used instead of Compound a. Bn))-SRIF28) (SEQ ID NO: 135) was synthesized.
  • 60-2 Sugar chain modification reaction of thiol Peptide 1 (10.4 mg, 3.14 ⁇ mol) was dissolved in 0.5 M phosphate buffer (pH 7.4, 62 ⁇ L) containing 30 ⁇ M TCEP. To this solution, a solution of compound q (79.4 mg, 28.5 ⁇ mol) obtained by the method described in 60-1 above in DMSO (3.7 mL) was added and reacted at room temperature for 20 minutes.
  • Example 62 Synthesis of S1-2C (disialo (Bn))-SRIF28 In the same manner as in Example 28 except that compound g was used instead of compound c, compound (S1- 2C (disialo (Bn))-SRIF28) (SEQ ID NO: 140) was synthesized.
  • the peptide 138 (SEQ ID NO: 142) represented by the following formula (138), which is protected and bound to the resin by the peptide solid phase synthesis method by the Fmoc method, using a Prelude (trademark) peptide synthesizer ) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was then removed by treatment with 20% piperidine in DMF. After washing with DMF, compound d (141.0 mg, 51.5 ⁇ mol), DMSO-DMF (1/1, v / v, 1.1 mL) solution, TBTU (21.2 mg, 66.0 ⁇ mol), and DIPEA (17 .2 ⁇ L, 98.7 ⁇ mol) were sequentially added to the resin and condensed by shaking at room temperature for 4 hours. After washing with DMF, this condensation operation was repeated once.
  • the resin is washed with DMF and dichloromethane, and then shaken with 20% piperidine in DMF for 20 minutes to remove the Fmoc group, and the resin is washed with DMF, whereby the resin is bound to the resin represented by the following formula (139).
  • glycopeptide 140 (SEQ ID NO: 144) (5.8 mg, 1.1 ⁇ mol) represented by the following formula (140).
  • ESI-MS (m / z) calcd for C 241 H 367 N 49 O 101 S 4 : [M + 4H] 4+ 1424.8, [M + 5H] 5+ 1140.0, found 1424.6, 1139.9.
  • Glycopeptide 142 (3.7 mg, 0.54 ⁇ mol) obtained by the method described in 64-3 above was added to 100 mM Tris-HCl buffer (pH 8.0) -DMSO (1/1, v / v, 1.4 mL) and reacted at room temperature for 31 hours.
  • Example 65 Synthesis of C (disialo (aminoethylamide)) ⁇ S1C (disialo) -SRIF28 65-1 Synthesis of peptide Take 2-chlorotrityl chloride resin (100 ⁇ mol) in a column for solid phase synthesis, and wash with DMF and dichloromethane. A solution of Fmoc-Cys (Acm) -OH (49.7 mg, 120 ⁇ mol) and DIPEA (104.5 ⁇ L, 600 ⁇ mol) in dichloromethane (3.0 mL) was added and shaken for 1 hour. After washing with dichloromethane and DMF, the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the protected peptide 145 represented by the following formula (145) in a state bound to the resin by the peptide solid phase synthesis method by the Fmoc method using a Prelude TM peptide synthesizer (SEQ ID NO: 149) ) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • a portion of the resin (50 ⁇ mol) was taken and the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • 65-3 Sugar chain modification of thiol Peptide 146 (20.6 mg, 5.89 ⁇ mol) obtained by the method described in 65-1 above and compound s (28.6 mg) obtained by the method described in 65-2 above 11.8 ⁇ mol) was dissolved in 33 mM phosphate buffer (pH 7.4, 1.8 mL) and reacted at room temperature for 30 minutes.
  • a protected peptide 152 (sequence) in a state bound to a resin represented by the following formula (152) by a peptide solid-phase synthesis method by Fmoc method using a Prelude (trademark) peptide synthesizer No. 156) was synthesized.
  • the condensation reaction was performed in DMF using HCTU as the condensing agent.
  • the Fmoc protecting group was removed by treatment with 20% piperidine in DMF.
  • the resin was removed by filtration, and cooled diethyl ether was added to the filtrate to obtain a crude peptide as a precipitate.
  • Tables 1-1 to 1-7 show the MS spectrum data (ESI-MS) of the glycosylated SRIF peptides obtained by the methods described in Examples 1 to 66.
  • the molecular mass was obtained by performing deconvolution of multivalent protein mass spectrometry using MassLynx version 4.1 (manufactured by Waters).
  • Example 67-1 Calculation of Receptor Binding Affinity
  • Reagents used in competitive binding experiments and their official chemical names are as follows.
  • HEPES 4- (2-hydroxyethyl) -1-piperazine etheric acid
  • BSA bovine serum albumin
  • Competitive binding experiments were outsourced to Lyselka Biosciences for experiments and data analysis.
  • the receptor subtypes and receptor membrane preparations used are shown in Table 2.
  • SRIF14 was used as a positive control for each experimental round.
  • 50% inhibitory concentration (IC 50 value) was obtained based on the numerical data of the binding inhibition rate by non-linear least square method using MathIQ TM (ID Business Solutions, UK).
  • the binding inhibition constant (Ki value) was calculated by the method of Cheng, Y et al. (Biochem Pharmacol, 22, 3099-3108, 1973).
  • Table 3A the compounds according to the present invention bound strongly to all SSTRs.
  • the binding affinity of the positive control SRIF14 to SSTR1 was 0.362 nM in Ki value, whereas the compound having one or two sugar chains of the present invention has an excellent binding affinity of 0.704 to 147 nM. Showed sex.
  • the compounds having three sugar chains of the present invention (the compounds of Examples 24, 25, and 29) also showed 3.49 nM, 16.9 nM, and 57.3 nM, and had excellent binding affinity. It was.
  • Examples 1 to 6, 10, 13 to 19, 26, 27, 30, and 31 have one sugar chain having binding affinity for all receptors of SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5. It was found to be a modified form.
  • Examples 20 to 23 and 28 are modified sugar chains having affinity for all receptors of SSTR1 to SSTR5.
  • Examples 24, 25 and 29 are examples of SSTR1 to SSTR5. It was found to be a modified three sugar chain having affinity for all receptors.
  • Example 67-2 Calculation of receptor binding affinity-2
  • Each compound shown in Table 3B was subjected to a competitive binding experiment by the method described in Example 67-1, and the receptor binding affinity was calculated.
  • SRIF14 and SRIF28 were similarly evaluated as control compounds.
  • the results of the binding experiment are shown in Table 3B.
  • 1C and 1D show an example of the structure of a glycosylated peptide corresponding to the compound name in Table 3B.
  • control SRIF14 and SRIF28 bound to all receptors of SSTR1-SSTR5.
  • the Ki value for each receptor of SRIF14 is different and is 2.5 to 13.2 times higher.
  • the compounds 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65 and 66 have Ki values for SSTR1 of 1.46 to 100 nM, and also 0.0536 to 6.51 nM for SSTR2.
  • SSTR3 was 0.160-7.44 nM
  • SSTR4 was 1.39-59.0 nM
  • SSTR5 was 0.310-95.0 nM, indicating high binding affinity for all receptors.
  • the compound of Example 12 had Ki values for SSTR2 and SSTR5 of 280-1600 times that of SRIF14, but 17.9, 7.44, and 24.1 nM for SSTR1, SSTR3, and SSTR4. , And was thought to retain binding affinity.
  • the compound of Example 38 had a Ki value for SSTR1, SSTR2, and SSTR3 of 310-5300 times that of SRIF14, which was significantly reduced in affinity, but was 110, 33.0 nM for SSTR4 and SSTR5. It was thought to retain binding affinity.
  • the compound of Example 49 had Ki values of SSTR2 and SSTR4 of 170 times and 46 times or more of SRIF14, but Ki values of 270 nM, 14.0 nM and 23.0 nM for SSTR1, SSTR3 and SSTR5, respectively. And was thought to retain binding affinity.
  • the somatostatin receptor is a G protein coupled receptor (GPCR). SSTR1 to SSTR5 all suppress adenylyl cyclase activity and reduce intracellular cAMP concentration via Gi / Go, a subfamily of G proteins. In this experimental system, each somatostatin receptor-expressing cell was used to calculate the IC 50 value of the cAMP production inhibitory action of the test substance, and the agonist activity was evaluated. In addition, SRIF14 and SRIF28 were similarly evaluated as control compounds. The reagents used in this experiment and their official chemical names are as follows.
  • DMEM Dulbecco's Modified Eagle's Medium
  • IBMX 3-isobutyl-1-methylxanthine
  • HBSS Hank's Buffered Salt Solution
  • the test substance is treated at a concentration of 0.00001 nM, 0.0001 nM, 0.001 nM, 0.01 nM, 0.1 nM, 1 nM, 10 nM, 100 nM, or 1000 nM mixed with 10 ⁇ M forskolin and allowed to react at room temperature for 30 minutes. It was. After the reaction, the cells were lysed with 0.2N HCl, and the amount of cAMP accumulated in the cells was measured using a Cayman cyclic AMP EIA kit (Cayman, 582002). The evaluation for SSTR1 was outsourced to SELEP to conduct experiments.
  • HBSS containing 20 mM HEPES (pH 7.4) and 500 ⁇ M IBMX was used, and SSTR1 receptor-expressing cells were seeded at 10 4 cells / well.
  • the test substance was treated by mixing with 1 ⁇ M NKH477 at a concentration of 0.001 nM, 0.01 nM, 0.1 nM, 1 nM, 10 nM, or 100 nM and allowed to react at 37 ° C. for 20 minutes. After the reaction, the amount of cAMP accumulated in the cells was measured using a cAMP dynamic2 kit (cisbio, 62AM4PE) manufactured by Sysbio.
  • cAMP dynamic2 kit cisbio, 62AM4PE
  • Table 3D and FIG. 1E show the experimental results (IC 50 (nM)) of the agonist activity evaluation test when the compounds of Examples 1, 18, 27, 28, 57 and 58 were used as the sugar chain adduct.
  • the IC 50 of the control compounds SRIF14 and SRIF28 is 0.83-0.89 nM for SSTR1, 0.0076-0.073 nM, 0.029-0.21 nM for SSTR2, SSTR3, SSTR4, SSTR5, respectively. They were 0.015 to 0.074 nM and 0.066 to 0.12 nM.
  • the IC 50 of the agonist activity of SDR1, SSTR2, SSTR3, SSTR4, SSTR5 of the compound which is a glycosylated adduct is 1.0 to 2.4 nM, 0.041 to 0.10 nM, 0.12 to 0.30 nM, 0 0.039 to 0.085 nM and 0.043 to 0.081 nM, showing excellent agonist activity for all receptors of SSTR1 to SSTR5. From the results shown in Example 67-1 and Example 67-2, it is clear that these compounds have receptor binding ability, and these compounds exert agonist activity by binding to the receptor. Became clear.
  • Example 67-4 Agonist Activity Evaluation Using Receptor-Expressing Cells-2 Using each compound shown in Table 3E as the glycosylated product, an agonist activity evaluation test was conducted in the same manner as in Example 67-3. The experimental results are shown in Table 3E. In Table 3E, a column in which “-(hyphen)” is written indicates that the operation is not performed.
  • the IC 50 of the agonist activity with respect to SSTR1, SSTR2, SSTR3, SSTR4, and SSTR5 of the compound that is a glycosylated is 1.2-22 nM, 0.019-1.4 nM, and 0.039-3. It was 8 nM, 0.033 to 1.6 nM, and 0.031 to 1.1 nM, and showed agonistic activity against the receptors of SSTR1 to SSTR5. From the results shown in Example 67-1 and Example 67-2, these compounds have receptor binding affinity, and these compounds exhibit agonist activity by binding to the receptor. It became clear.
  • Example 68 Pharmacokinetic study 1 using rats Compared with non-glycosylated SRIF28, the glycosylated polypeptide (glycosylated product) of the present invention has an area under the drug plasma concentration-time curve (AUC), blood half-life (t 1/2 ), In order to confirm that pharmacokinetic profiles such as mean residence time (MRT) and bioavailability were improved, pharmacokinetic analysis by intravenous administration and subcutaneous administration was performed using rats.
  • AUC drug plasma concentration-time curve
  • t 1/2 blood half-life
  • the aprotinin-containing EDTA-PBS solution was prepared by dissolving aprotinin (010-11834, manufactured by Wako Pure Chemical Industries, Ltd.) with EDTA-PBS to a concentration of 0.142 mg / mL, and used as an anticoagulant for collected blood.
  • the administration liquid prepared in 68-1 was administered at a dose of 1 mL / kg under non-fasting conditions using a syringe and a 26G injection needle (both manufactured by Terumo) (40 nmol / kg as S1C (disialo) -SRIF28).
  • Blood was collected from the rat cervical vein before administration, 2 minutes, 5 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, and 8 hours after administration.
  • the collected blood (0.2 mL) was immediately mixed with 0.2 mL of the aprotinin-containing EDTA-PBS solution prepared in 68-1 above, and left on ice for 30 minutes or more. After centrifugation (1,870 ⁇ g, 4 ° C., 10 minutes), 250 ⁇ L of the supernatant was collected and used as a plasma sample. As blank plasma, plasma obtained by similarly treating blood collected from an untreated rat cervical vein was used. Plasma samples were stored frozen until used for measurement. In addition, the chip
  • 68-3 Plasma Concentration Measurement S1C (disialo) -SRIF28 plasma concentration measurement in the plasma sample obtained in 68-2 above was performed using Phoenix Pharmaceuticals Inc. Somatostatin EIA kit (Phoenix Pharmaceuticals Inc, EK-060-03). It was performed using. The plasma sample was diluted 5 times, 20 times, 100 times, 400 times, 1600 times using the assay buffer attached to the EIA kit, and used as a measurement sample. A standard solution for preparing a calibration curve was prepared as follows.
  • the blank plasma obtained in 68-2 above is diluted in the same manner as the plasma sample using the assay buffer attached to the EIA kit, and used as an assay buffer for preparing a standard solution (for example, when the plasma sample is diluted 100 times).
  • S1C (disialo) -SRIF28 was diluted with a PBS solution to prepare a 100 ⁇ M solution, and a 2 ⁇ M solution was prepared from the 100 ⁇ M solution.
  • the obtained 2 ⁇ M S1C (disialo) -SRIF28 solution was serially diluted with an assay buffer for preparing a standard solution to prepare 20 nM, 10 nM, 2 nM, 0.4 nM, 0.08 nM, and 0.04 nM standard solutions.
  • the plasma concentration was calculated by multiplying the obtained result by the dilution rate and further by the dilution rate 2 with the aprotinin-containing EDTA-PBS solution as the anticoagulant treatment.
  • the same operation was performed using non-glycosylated SRIF28 instead of the glycosylated product.
  • FIG. 2 shows the time course of S1C (disialo) -SRIF28 concentration in the obtained plasma.
  • AUC was calculated by the trapezoidal method from the obtained S1C (disialo) -SRIF28 concentration transition using the moment analysis method. Further, the maximum plasma concentration (C max ) was determined by extrapolation from the predicted initial concentration (C 0 ) upon intravenous administration, and further from t 1/2 , MRT, and the actual measurement values upon subcutaneous administration. The resulting pharmacokinetic parameters are shown in Table 4. As can be seen from the results shown in FIG. 2 and Table 4, S1C (disialo) -SRIF28 has a significant increase in t1 / 2 and MRT compared to non-glycosylated SRIF28, and AUC and Cmax . An increase was observed.
  • sugar chain adducts have improved in vivo stability compared to non-sugar chain adducts.
  • the increase in AUC and Cmax is considered to be due to the improvement in stability in the living body.
  • Example 69 Pharmacokinetic Study 2 Using Rats A pharmacokinetic test was carried out in the same manner as in Example 68 except that S1C (disialo) -SRIF28, N5C (disialo) -SRIF28 and S1C (disialo) N5C (disialo) -SRIF28 were used as the glycosylated adduct. . As a control, non-glycosylated SRIF28 was used instead of the glycosylated product. The compound plasma concentration transition obtained is shown in FIG.
  • Example 70 Pharmacokinetic Study 3 Using Rats Example 68 Similarly, pharmacokinetic studies were performed. As a control, non-glycosylated SRIF28 was used instead of the glycosylated product.
  • FIG. 4 shows the compound concentration transition in the obtained plasma.
  • Example 71 Pharmacokinetic study 4 using rats S1C (disialo) -SRIF28, N5C (disialo) -SRIF28, A9C (disialo) -SRIF28, S1C (disialo) N5C (disialo) -SRIF28, N5C (disialo) A9C (disIFo) And S1C (disialo), N5C (disialo), A9C (disialo) -SRIF28, and the pharmacokinetic study was performed in the same manner as in Example 68. The resulting plasma compound concentration transition is shown in FIG.
  • Example 72 Pharmacokinetic study 5 using rats 5 A pharmacokinetic study was performed in the same manner as in Example 68 except that S1C (disialo) -SRIF28, S1-2C (disialo) -SRIF28, and S1-3C (disialo) -SRIF28 were used as the glycosylated adduct. . The resulting plasma compound concentration transition is shown in FIG.
  • BA (%) (AUC (sc) / Dose (sc) ) / (AUC (iv) / Dose (iv) ) AUC (sc) : AUC at the time of subcutaneous administration (min ⁇ nM) Dose (sc) : Dose in subcutaneous administration (nmol / kg) AUC (iv) : AUC at the time of intravenous administration (min ⁇ nM) Dose (iv) : Dose in intravenous administration (nmol / kg)
  • the bioavailability of the sugar chain adduct of the present invention increased as the number of modified sugar chains increased. That is, the non-glycosylated adduct was 5%, but the glycosylated polypeptide with one sugar chain added was 37-60%, and the average was 50%. Two sugar chains were added at intervals. It was found that the increase was 77-85%, an average of 81%, and that of 3 sugar chains added at intervals was 91%. In addition, comparing the ones with closely spaced sugar chains, it was found that the number with one added increased to 37%, the number with two added 55%, and the number with three added increased to 69%. (Compounds of Examples 1, 20, and 24).
  • bioavailability is improved by the sugar chain modification according to the present invention.
  • One of the causes may be the stability of the compound in the blood or the transferability (from the administration site) into the blood.
  • AUC at the time of subcutaneous administration which is an index for inferring blood transferability at the time of subcutaneous administration, increases with an increase in the number of modified sugar chains (1: 2209 min ⁇ nM, 2 (with intervals): 3400 min ⁇ nM, 3 (with intervals): 4015 min ⁇ nM), and it was speculated that the improvement in blood migration contributed to the increase in bioavailability.
  • t 1/2 and MRT prolongation effect for example, t 1/2 for intravenous administration, 1: 19.0 min, 2 ( (With spacing): 27.2 min, 3 (with spacing): 39.8 min), and stability in blood was estimated to be improved.
  • AUC at the time of intravenous administration does not increase relative to the number of modified sugar chains (1: 4453 min ⁇ nM, 2 (with intervals): 4198 min ⁇ nM, 3 (with intervals) : 4402 min ⁇ nM), it is considered that only an increase in blood stability due to an increase in the number of modified sugar chains of the compound does not contribute to an increase in bioavailability.
  • the dense compound 20 has 7306 min ⁇ nM compared to 4029-4465 min ⁇ nM of the compound 21, compound 22 and compound 23 with a gap, and in the case of the triple sugar chain, the gap Compared to 4402 min ⁇ nM of Compound 25, the density of Compound 24 is 5660 min ⁇ nM.
  • the bioavailability was improved compared to the case where the distance between the sugar chain modification positions was closer.
  • the compound 21, compound 22, and compound 23 with a gap are 77 to 85% (average value 81%) compared to 55% of the dense compound 20, and the three sugar chains In this case, the compound 25 with a gap is 91% compared to 69% of the dense compound 24. From these facts, it is considered that, as the position for sugar chain modification related to the present invention, the modification with a plurality of intervals at intervals rather than the modification with a plurality of densely contributes to the improvement of bioavailability.
  • Example 73 Pharmacokinetic study using rats 7 The pharmacokinetics test was carried out in the same manner as in Example 68 except that S1C (disialo) -SRIF28, S1C (disialo (amide))-SRIF28, and S1C (disialo (aminoethylamide))-SRIF28 were used as the glycosylated adduct. Carried out. The resulting plasma compound concentration transition is shown in FIG.
  • Example 74 Pharmacokinetic study 8 using rats The pharmacokinetic test was carried out in the same manner as in Example 68 except that S1C (disialo) -SRIF28, S1C (disialo (Bn))-SRIF28, and S1C (disialo) -D-Trp22-SRIF28 were used as the glycosyl adduct. Carried out. The resulting plasma compound concentration transition is shown in FIG.
  • Example 75 Pharmacokinetic study using rats 9 The pharmacokinetic test was carried out in the same manner as in Example 68 except that S1C (disialo) -SRIF28, R13C (disialo) -SRIF28, and K14C (disialo) -SRIF28 were used as the glycosylated adduct. The resulting plasma compound concentration transition is shown in FIG.
  • Example 76 Pharmacokinetic study 10 using rats 10 S1C (disialo) -SRIF28, E12C (disialo) -SRIF28, N19C (disialo) -SRIF28, 29C (disialo) -SRIF28, S1C (monosialo) -SRIF28, S1C (asialo) -SRIF28 were used as the glycosylation products.
  • a pharmacokinetic test was carried out in the same manner as in Example 68 except that. The compound plasma concentration transition obtained is shown in FIG.
  • Example 77 Pharmacokinetic Study 11 Using Rats 11 A pharmacokinetic test was carried out in the same manner as in Example 68 except that S1C (disialo) -SRIF28, K14C (disialo) -SRIF28, and C (disialo) -SRIF14 were used as the glycosylated adduct. The compound plasma concentration transition obtained is shown in FIG.
  • Example 78 Pharmacokinetic study 12 using rats 12 A pharmacokinetic test was carried out in the same manner as in Example 68 except that C (disialo) -SRIF14, C (disialo) -C12linker-SRIF14, and C (disialo) -PEGlinker-SRIF14 were used as the glycosyl adduct. The resulting plasma compound concentration transition is shown in FIG.
  • Example 79 Pharmacokinetic study using rats 13 Example 68 except that SRIF28, S1C (disialo) -SRIF28, S1C (asialo) -SRIF28, S1C (diGlcNAc) -SRIF28, S1C (diMan) -SRIF28, S1C (GlcNAc) -SRIF28 were used as the glycosylated adduct A pharmacokinetic study was performed in the same manner as described above. The compound plasma concentration transition obtained is shown in FIG.
  • Example 80 Pharmacokinetic Study 14 Using Rats Except that S1C (disialo) -SRIF28, S1C (tetrasialo) -SRIF28, S1C (trisialo) -SRIF28, S1C (Asn (disialo))-SRIF28, S1-2C (disialo) -SRIF28 were used as glycosylation products.
  • a pharmacokinetic test was performed in the same manner as in Example 68. The compound plasma concentration transition obtained is shown in FIG.
  • Example 81 Pharmacokinetic study 15 using rats 15 Example 68 except that SRIF28, S1C (disialo) -SRIF28, S1-2C (disialo) -SRIF28, S1-3C (disialo) -SRIF28, S1-4C (disialo) -SRIF28 were used as the glycosylated adduct. In the same manner as described above, a pharmacokinetic study was performed. The compound plasma concentration transition obtained is shown in FIG.
  • Example 82 Pharmacokinetic study 16 using rats 16 SRIF14, C (disialo) -SRIF14, C (disialo) -K-SRIF14, C (disialo) -RK-SRIF14, 2C (disialo) -RK-SRIF14, 3C (disialo)- A pharmacokinetic test was performed in the same manner as in Example 68 except that RK-SRIF14 was used. The compound plasma concentration transition obtained is shown in FIG.
  • Example 83 Pharmacokinetic study 17 using rats The pharmacokinetics test was carried out in the same manner as in Example 68 except that S1C (asialo) -SRIF28, S1-2C (asialo) -SRIF28, and S1-3C (asialo) -SRIF28 were used as the glycosyl adduct. The compound plasma concentration transition obtained is shown in FIG.
  • the pharmacokinetic parameters of each compound were calculated in the same manner as in Example 68 from the plasma compound concentration transition obtained in the pharmacokinetic tests 7 to 18 of Examples 73 to 84.
  • the resulting pharmacokinetic parameters are shown in Tables 5B-5F.
  • S1C (disialo) -D-Trp22-SRIF28 is 13 to 18 times longer by t 1/2 and AUC is 8 to 23 times compared to non-glycosylated SRIF28 in intravenous administration Increased.
  • C (disialo) -SRIF14, C (disialo) -K-SRIF14, and C (disialo) -R-K-SRIF14 are t 1/2 when administered intravenously as compared to SRIF14 which is a non-glycosylated adduct. Increased 33-38 times and AUC increased 44-55 times. Also, C (disialo) -C12linker-SRIF14 , C (disialo) -PEGlinker-SRIF14 , compared to SRIF14 non glycosylated body, t 1/2 is extended 22-33 times, AUC is 14-30 Doubled. That is, as in Example 68, it was considered that the modification in the sugar chain improved the stability in blood.
  • the size of the sugar chain to be modified is changed to GlcNAc (monosaccharide), diMan (pentasaccharide), diGlcNAc (7 saccharide), asialo (9 saccharide), monosialo (10 saccharide),
  • the t 1/2 , AUC, and bioavailability at the time of subcutaneous administration depend on the size of the sugar chain to be modified. Tee increased 2 to 17 times, 3 to 120 times, and 2 to 11 times, respectively.
  • sugar chain to be modified not only improves blood stability, but also changes the rate of increase.
  • dimannose sugar chains and asialo sugar chains are known to interact with specific proteins, so they can also be used to target specific proteins or organs or cells that have specific proteins. It is considered that it can be used.
  • sialic acid at the non-reducing end for example, S1C (disialo (amide))-SRIF28, S1C (disalo) added with a sugar chain whose charge has been changed by introducing aminoethylamide, amide, Bn or the like into a carboxy group (aminoethylamide)) - SRIF28, S1C (disialo (Bn)) - SRIF28 is compared to non-glycosylated member in intravenous administration, t 1/2 is extended 11-14 times, AUC is increased by 7 to 12 times And blood stability was improved.
  • SRIF14 was modified with a single disialo sugar chain C (disialo) -RK-SRIF14, two modified 2C (disialo) -RK-SRIF14, three Modified 3C (disialo) -RK-SRIF14 is 38,63,71 times longer by t1 / 2 compared to non-glycosides on intravenous administration, and AUC is 55,42, Increased 36 times.
  • SRIF28 was modified with S1C (disialo) -SRIF28 with one modified disialo sugar chain, 2 modified S1-2C (disialo) -SRIF28, 3 modified S1-3C (disialo) -SRIF28, 4 modified S1-4C (disialo) -SRIF28 has an increase in t 1/2 of 13, 21, 30, 48 times, respectively, and AUC of 11, 12, 12, Increased 15 times. In both cases of SRIF14 and SRIF28, it was revealed that blood stability was improved according to the number of disialo sugar chains to be modified.
  • S1-2C (disialo) -SRIF28, S1-2C (asialo) -SRIF28, C (disialo (aminoethylamide)) ⁇ S1C (disilo) -SRIF28, S1-2C (disialo) (Bn))-SRIF28, S1-2C (disialo (amide))-SRIF28 is increased by 6 to 21 times in t 1/2 compared to non-glycosylated adducts when administered intravenously, Increased 6-12 times. That is, the blood stability was improved by modifying the sugar chain to SRIF28.
  • Example 85 Plasma stability test using rat plasma 85-1
  • treatment solution reagent and rat plasma Glycoadduct and non-glycosylated SRIF28 were dissolved in PBS solution to prepare 2 ⁇ M solution, and treatment solution It was.
  • 10% TFA was prepared by dissolving trifluoroacetic acid (manufactured by Wako Pure Chemical Industries, Ltd., 208-02746) with water so as to be 10 v / v%.
  • Rat plasma was prepared from Wistar rats (Crlj: Wistar, male, Nippon Charles River Co., Ltd., 7 weeks old) as heparin-added plasma (heparin: Japanese Pharmacopoeia Heparin Sodium Injection (Mochida Pharmaceutical)).
  • the sampling time was 0 hour, 1 hour, 2 hours, 4 hours, or 0 hour, 4 hours, 8 hours, and 24 hours.
  • plasma obtained by the same treatment was used except that a PBS solution was used as a treatment liquid. Plasma samples were stored frozen until used for measurement. In each experiment, non-glycosylated SRIF28 was used as a positive control.
  • the glycosylated polypeptide of the present invention has increased plasma stability compared to SRIF28. That is, in the case where one sugar chain is added to the 1st, 5th, 9th and 12th positions (compounds of Examples 1, 2, 3 and 4), it is 4.5 to 6.7 times that of SRIF28. 15.9 times the sugar chain added at position 13 (compound of Example 5), 19.1 times the sugar chain added at position 14 (compound of Example 6), and the sugar chain at position 30 16.8 times (compound of Example 14). Moreover, in the case where one additional sugar chain-added amino acid was further added to the 19th position and the C-terminal side, the number was 20 times or more (compounds of Examples 10 and 13). It was shown that the position where the sugar chain is added increases the plasma stability from the 1st position toward the C-terminal side.
  • Example 86 GH Production Inhibition Test Using Rats
  • the glycosylated polypeptide of the present invention had affinity for each receptor of SSTR.
  • the affinity for each SSTR was attenuated, but even in such a case, the receptors in vivo were increased by extending the blood half-life and increasing bioavailability shown in Examples 68 to 85.
  • an in vivo test using rats was conducted to evaluate the administration effect of the glycosylated polypeptide of the present invention on growth hormone (GH) production. did.
  • the increase in the amount of GH produced in the blood is thought to affect the biological reaction by causing cell proliferation, differentiation and activation of biosynthetic systems in various organs through the paracrine action of GH. It is done. Somatostatin released from the hypothalamus suppresses GH secretion into the blood from the anterior pituitary gland.
  • This experimental system was carried out as a test system capable of evaluating the pharmacological action of the glycosylated form on SSTR and the residual in blood after administration of the glycosylated form, using blood GH production as an index.
  • 0608101 manufactured by Kyoritsu Pharmaceutical Co., Ltd.
  • a general anesthetic is 50 mg / kg. It was administered intraperitoneally using an injection needle.
  • GRF was administered as a GH release promoter at a dose of 1 mL / kg using a syringe and a needle at a dose of 1 mL / kg 1 hour after administration of the glycosylated, that is, after 50 minutes or more under anesthesia.
  • Five minutes after administration of GRF blood was collected from a rat neck vein using a syringe and a needle containing heparin.
  • Plasma samples were stored frozen until used for measurement.
  • Plasma GH Concentration GH concentration in the plasma sample obtained in the above 86-2 was measured using SPI-Bio Rat GrowthHorne EIA kit (SPI-Bio, A05104). The plasma sample was diluted 20 times, 100 times, and 500 times using the assay buffer attached to the EIA kit to obtain a measurement sample.
  • a standard solution for preparing a calibration curve was prepared by preparing a 40 ng / mL solution with distilled water according to the attached instructions, and then serially diluting with an assay buffer to obtain 20 ng / mL, 10 ng / mL, 5 ng / mL, 2 0.5 ng / mL, 1.25 ng / mL, 0.63 ng / mL, and 0.31 ng / mL were prepared.
  • the GH concentration was calculated by multiplying the obtained result by the dilution rate. Table 7 shows the GH concentration in the obtained plasma sample.
  • the glycosylated product of the present invention suppressed GH production in rats.
  • the glycosylated adducts of Example 1, Example 10, and Example 13 were measured by the method of Example 67-1 and the ratio between the Ki value for each receptor and the Ki value of SRIF14 having no sugar chain In the range of 100: 1 to 1.3: 1.
  • the sugar chain adduct of Example 1 is shown to have a blood half-life increased by 10 times or more when measured by the method of Examples 68 to 72. From this example, it was shown that the sugar chain adduct effectively exerts pharmacological action even in vivo.
  • the sugar chain adduct of the present invention had a GH production inhibitory action even when administered 1 hour before GRF administration. Moreover, it was shown that the effective dose with respect to rat GH production ability differs about 10 times in Example 1 and the glycosylated form of Example 10 and Example 13. This is similar to the fact that when measured by the method of Example 67-1, their receptor affinity is about a 10-fold difference in Ki value. Even when the affinity of the glycosylated adduct was somewhat attenuated, it was shown to have pharmacological activity.
  • Example 87 GH Production Inhibition Test 2 Using Rats In the same manner as in Examples 86-1 to 86-3, a rat GH production inhibition test was carried out on the following compound, which is a sugar chain adduct.
  • the glycosylated polypeptide of the present invention suppressed GH production in rats.
  • S1C (disialo) -SRIF28 suppressed GH production from 1 nmol / kg and showed 82-99% GH production suppression effect at 3-10 nmol / kg. This is presumably because, as shown in Examples 67-1 and 67-2 and Examples 68 to 85, it has affinity for SSTR1 to SSTR5 and the retention in blood is improved.
  • Example 88 Drug Efficacy Test in Gastrointestinal Obstruction Model As shown in Examples 86 and 87, it was proved that the glycosylated polypeptide of the present invention effectively acts as an agonist in the living body of rats.
  • an evaluation in a rat gastrointestinal obstruction model was performed. In gastrointestinal obstruction such as ileus, gastrointestinal symptoms such as abdominal fullness, vomiting, and abdominal pain occur due to tissue damage to the gastrointestinal tract and decreased absorption of water and electrolytes. It is known that the pathological condition is caused by obstruction of the contents of digestive tract contents or release of digestive juice or physiologically active substance into the digestive tract.
  • Somatostatin is effective in improving symptoms by suppressing the secretion of various digestive fluids via SSTR expressed in the digestive system or reducing the contents of the digestive tract by promoting absorption of water and electrolytes.
  • This experimental system was carried out as a test system for evaluating the intestinal fluid absorption promotion or secretion inhibitory effect using the change in the weight of the intestinal fluid in the jejunum after intestinal obstruction as an index.
  • blood parameters of amylase (pancreas), lactate dehydrogenase (LDH, liver), and creatine phosphokinase (CPK, skeletal muscle, myocardium, etc.), which are deviating enzymes were measured as indices at the time of tissue damage.
  • Example 88-1 Creation of Gastrointestinal Obstruction Model This test was commissioned to Mitsubishi Chemical Courtce Corporation.
  • a midline incision was made in the abdomen, and the jejunum at about 10 cm from the duodenal posterior muscle was ligated with a suture. Thereafter, the incision was quickly sutured and fasted until compound administration.
  • the jejunum was not ligated, and a midline incision was made in the abdomen and then the incision was sutured.
  • Example 88-2 Compound Preparation and Administration Japanese Pharmacopoeia Saline Solution Using S1C (disialo) -SRIF28, C (disialo) -RK-SRIF14, S1-2C (asialo) -SRIF28 as a glycosyl adduct It was dissolved in (manufactured by Otsuka Pharmaceutical Factory Co., Ltd.) to prepare a 40 ⁇ M solution, which was used as an administration solution. 18 hours after the gastrointestinal occlusive surgery, it was subcutaneously administered to the back of the neck using a syringe and a 25G injection needle (both manufactured by Terumo) at a dose of 1 mL / kg. As the Vehicle group, a physiological saline solution containing no glycosylated polypeptide was administered in the same manner. In addition, octreotide was administered as a control.
  • Example 88-3 Measurement of Intestinal Fluid Weight
  • a midline incision was made in the abdomen under inhalation anesthesia, and 1.5 mL of blood was collected from the abdominal vena cava. Then, the ligated jejunum on the duodenal ligament side was removed. The liquid and blood on the jejunum surface were removed with a paper towel, and nerves, blood vessels, and fats attached to the jejunum were excised, then cut to a length of 6 cm, and wet weight was measured. Then, it was dried at 36 degrees for 24 hours, and the dry weight was measured. Intestinal fluid weight (mg) was calculated as wet weight-dry weight. Table 9 shows the weight of the jejunum obtained.
  • S1C (disialo) -SRIF28, C (disialo) -RK-SRIF14, and S1-2C (asialo) -SRIF28 all showed an increase in intestinal fluid weight as compared to vehicle, and the glycosylated adduct of the present invention.
  • S1-2C (asialo) -SRIF28 had half the AUC upon subcutaneous administration in Examples 83 and 84 compared to S1C (disialo) -SRIF28.
  • Example 88-4 Measurement of blood parameters Using the blood collected in Example 88-3, the amylase concentration (IU / L), LDH concentration (IU / L), and CPK concentration (IU / L) were automatically analyzed 7170. (Hitachi). As the measurement method, BG5B method, UV-rate method and JSCC method were used, respectively. Table 10 shows the obtained results.
  • Octreotide is a compound having specific affinity for SSTR2, SSTR3, and SSTR5, while having binding affinity for the receptor. Since there is a report that all receptors of SSTR1 to SSTR5 are expressed in rat pancreas (J Histochem Cytochem. 2004 Mar; 52 (3): 391-400.), Glycosylated adduct is octreotide The possibility that the tissue damage was reduced by acting on a receptor different from that of. In addition, octreotide and C (disialo) -RK-SRIF14 had a low LDH activity compared to vehicle. In addition, none of the parameters deteriorated by administration of the glycosylated product.

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PCT/JP2012/072380 2011-09-04 2012-09-03 糖鎖付加ポリペプチドおよび当該ポリペプチドを含む医薬組成物 WO2013032011A1 (ja)

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BR112014004784A BR112014004784A2 (pt) 2011-09-04 2012-09-03 polipeptídeo glicosilado e composição de drogas contendo referido polipeptídeo
KR1020147008130A KR102031998B1 (ko) 2011-09-04 2012-09-03 당쇄 부가 폴리펩티드 및 당해 폴리펩티드를 포함하는 의약 조성물
EP12828271.2A EP2752424B1 (en) 2011-09-04 2012-09-03 Glycosylated somatostatin and drug composition containing it
RU2014112046A RU2624034C2 (ru) 2011-09-04 2012-09-03 Гликозилированные полипептиды и лекарственные композиции, содержащие данные полипептиды
JP2013531454A JP6178238B2 (ja) 2011-09-04 2012-09-03 糖鎖付加ポリペプチドおよび当該ポリペプチドを含む医薬組成物
CN201280053754.2A CN103930441B (zh) 2011-09-04 2012-09-03 附加糖链的多肽和含有该多肽的医药组合物
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DK12828271T DK2752424T3 (da) 2011-09-04 2012-09-03 Glycosyleret somatostatin og lægemiddelsammensætning, der indeholder det
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SG11201400289SA (en) * 2011-09-04 2014-05-29 Glytech Inc Glycosylated polypeptide and drug composition containing said polypeptide
CN111855827B (zh) * 2019-04-24 2022-09-16 岛津企业管理（中国）有限公司 多糖蛋白结合疫苗中多糖蛋白结合位点结合率的测定方法

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