WO2012161956A3 - Production de protéines à sous-unités multiples de pureté élevée telles que des anticorps dans des microbes transformés tels que pichia pastoris - Google Patents

Production de protéines à sous-unités multiples de pureté élevée telles que des anticorps dans des microbes transformés tels que pichia pastoris Download PDF

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Publication number
WO2012161956A3
WO2012161956A3 PCT/US2012/036953 US2012036953W WO2012161956A3 WO 2012161956 A3 WO2012161956 A3 WO 2012161956A3 US 2012036953 W US2012036953 W US 2012036953W WO 2012161956 A3 WO2012161956 A3 WO 2012161956A3
Authority
WO
WIPO (PCT)
Prior art keywords
subunit proteins
pichia pastoris
antibodies
purity production
transformed microbes
Prior art date
Application number
PCT/US2012/036953
Other languages
English (en)
Other versions
WO2012161956A2 (fr
WO2012161956A9 (fr
Inventor
Patricia Dianne Mcneill
Nicole Janson
Gary L. LESNICKI
Pei Qi
John A. Latham
Leon F. GARCIA-MARTINEZ
Original Assignee
Alder Biopharmaceuticals, Inc.
Alderbio Holdings Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2014512858A priority Critical patent/JP6193220B2/ja
Priority to SI201231967T priority patent/SI2710114T1/sl
Application filed by Alder Biopharmaceuticals, Inc., Alderbio Holdings Llc filed Critical Alder Biopharmaceuticals, Inc.
Priority to EP12789187.7A priority patent/EP2710114B1/fr
Priority to LTEPPCT/US2012/036953T priority patent/LT2710114T/lt
Priority to KR1020137033842A priority patent/KR101981662B1/ko
Priority to RS20211462A priority patent/RS62628B1/sr
Priority to EA201301294A priority patent/EA028913B9/ru
Priority to HRP20211873TT priority patent/HRP20211873T1/hr
Priority to BR112013029902A priority patent/BR112013029902A2/pt
Priority to CN201280035868.4A priority patent/CN103827290A/zh
Priority to PL12789187T priority patent/PL2710114T3/pl
Priority to CA2837000A priority patent/CA2837000C/fr
Priority to DK12789187.7T priority patent/DK2710114T3/da
Priority to NZ618635A priority patent/NZ618635B2/en
Priority to ES12789187T priority patent/ES2899381T3/es
Priority to MX2013013532A priority patent/MX368546B/es
Priority to SG2013084041A priority patent/SG194937A1/en
Publication of WO2012161956A2 publication Critical patent/WO2012161956A2/fr
Publication of WO2012161956A3 publication Critical patent/WO2012161956A3/fr
Publication of WO2012161956A9 publication Critical patent/WO2012161956A9/fr
Priority to IL229430A priority patent/IL229430B/en
Priority to CY20211101055T priority patent/CY1124796T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Cette invention concerne des procédés de production de protéines hétérologues à sous-unités multiples dans des cellules transformées. En particulier, cette invention concerne des procédés améliorés de production de protéines à sous-unités multiples, comprenant des anticorps et autres protéines à sous-unités multiples, qui peuvent être sécrétés ou pas, à un rendement plus élevé et une production réduite de sous-produits indésirables. Dans des modes de réalisation proposés en exemples, les cellules transformées sont une levure, par ex., une levure méthylotrophique telle que Pichia pastoris.
PCT/US2012/036953 2011-05-20 2012-05-08 Production de protéines à sous-unités multiples de pureté élevée telles que des anticorps dans des microbes transformés tels que pichia pastoris WO2012161956A2 (fr)

Priority Applications (19)

Application Number Priority Date Filing Date Title
MX2013013532A MX368546B (es) 2011-05-20 2012-05-08 Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.
CN201280035868.4A CN103827290A (zh) 2011-05-20 2012-05-08 多亚基蛋白质例如抗体在转化的微生物例如巴斯德毕赤酵母中的高纯度产生
EP12789187.7A EP2710114B1 (fr) 2011-05-20 2012-05-08 Production de protéines à sous-unités multiples de pureté élevée telles que des anticorps dans des microbes transformés tels que pichia pastoris
LTEPPCT/US2012/036953T LT2710114T (lt) 2011-05-20 2012-05-08 Gamyba aukšto grynumo iš keleto subvienetų sudarytų baltymų, tokių kaip antikūnai, transformuotuose mikroorganizmuose, tokiuose kaip pichia pastoris
KR1020137033842A KR101981662B1 (ko) 2011-05-20 2012-05-08 형질전환된 미생물 예컨대 피치아 패스토리스에서 다중-서브유닛 단백질 예컨대 항체의 고순도 생산
RS20211462A RS62628B1 (sr) 2011-05-20 2012-05-08 Proizvodnja visoke čistoće više-podjediničnih proteina kao što su antitela u preobraženim mikrobima kao što su pichia pastoris
EA201301294A EA028913B9 (ru) 2011-05-20 2012-05-08 Получение антител высокой чистоты в pichia pastoris
HRP20211873TT HRP20211873T1 (hr) 2011-05-20 2012-05-08 Proizvodnja visoke čistoće proteina s više podjedinica kao što su protutijela u transformiranim mikrobima kao što je pichia pastoris
CA2837000A CA2837000C (fr) 2011-05-20 2012-05-08 Production de proteines a sous-unites multiples de purete elevee telles que des anticorps dans des microbes transformes tels que pichia pastoris
JP2014512858A JP6193220B2 (ja) 2011-05-20 2012-05-08 形質転換されたピキア・パストリス（Ｐｉｃｈｉａｐａｓｔｏｒｉｓ）などの微生物における抗体などのマルチサブユニットタンパク質の高純度生産
PL12789187T PL2710114T3 (pl) 2011-05-20 2012-05-08 Wytwarzanie z wysoką czystością białek wielopodjednostkowych, takich jak przeciwciała, w transformowanych mikroorganizmach, takich jak pichia pastoris
BR112013029902A BR112013029902A2 (pt) 2011-05-20 2012-05-08 produção de alta pureza de proteínas de múltiplas subunidades, tal como anticorpos, em micróbios transformados, tal como pichia pastoris
DK12789187.7T DK2710114T3 (da) 2011-05-20 2012-05-08 Fremstilling med høj renhed af proteiner med multi-underenheder såsom antistoffer i transformerede mikrober såsom pichia pastoris
NZ618635A NZ618635B2 (en) 2011-05-20 2012-05-08 High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
ES12789187T ES2899381T3 (es) 2011-05-20 2012-05-08 Producción de alta pureza de proteínas de múltiples subunidades, tales como anticuerpos en microbios transformados, tal como Pichia pastoris
SI201231967T SI2710114T1 (sl) 2011-05-20 2012-05-08 Proizvodnja visoke čistote proteinov z več podenotami, kot so protitelesa v transformiranih mikrobih, kot je Pichia pastoris
SG2013084041A SG194937A1 (en) 2011-05-20 2012-05-08 High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
IL229430A IL229430B (en) 2011-05-20 2013-11-14 A highly purified production of multi-subunit proteins such as antibodies in genetically engineered microorganisms such as Pichia pastoris
CY20211101055T CY1124796T1 (el) 2011-05-20 2021-12-02 Παραγωγης υψηλης-καθαροτητας των πρωτεϊνων πολλαπλων-υπομοναδων οπως ειναι τα αντισωματα σε μετασχηματισθεντα μικροβια οπως ειναι to pichia pastoris

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201161488660P 2011-05-20 2011-05-20
US61/488,660 2011-05-20
US201161496860P 2011-06-14 2011-06-14
US201161496873P 2011-06-14 2011-06-14
US61/496,860 2011-06-14
US61/496,873 2011-06-14
US201161525307P 2011-08-19 2011-08-19
US61/525,307 2011-08-19

Publications (3)

Publication Number Publication Date
WO2012161956A2 WO2012161956A2 (fr) 2012-11-29
WO2012161956A3 true WO2012161956A3 (fr) 2013-01-17
WO2012161956A9 WO2012161956A9 (fr) 2013-09-06

Family

ID=47217961

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/036953 WO2012161956A2 (fr) 2011-05-20 2012-05-08 Production de protéines à sous-unités multiples de pureté élevée telles que des anticorps dans des microbes transformés tels que pichia pastoris

Country Status (21)

Country Link
EP (1) EP2710114B1 (fr)
JP (2) JP6193220B2 (fr)
KR (1) KR101981662B1 (fr)
CN (2) CN110408644A (fr)
BR (1) BR112013029902A2 (fr)
CA (1) CA2837000C (fr)
CY (1) CY1124796T1 (fr)
DK (1) DK2710114T3 (fr)
EA (1) EA028913B9 (fr)
ES (1) ES2899381T3 (fr)
HR (1) HRP20211873T1 (fr)
HU (1) HUE060418T2 (fr)
IL (1) IL229430B (fr)
LT (1) LT2710114T (fr)
MX (2) MX368546B (fr)
PL (1) PL2710114T3 (fr)
PT (1) PT2710114T (fr)
RS (1) RS62628B1 (fr)
SG (1) SG194937A1 (fr)
SI (1) SI2710114T1 (fr)
WO (1) WO2012161956A2 (fr)

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CN111973740A (zh) * 2014-03-21 2020-11-24 泰华制药国际有限公司 针对降钙素基因相关肽的拮抗剂抗体及其使用方法
TW202140776A (zh) * 2016-04-26 2021-11-01 美商美國泰福生技股份有限公司 細胞培養基
GB201803832D0 (en) * 2018-03-09 2018-04-25 Syndermix Ag Methods and compositions for lectin production
CN108913732B (zh) * 2018-07-31 2022-02-08 华东理工大学 一种莫纳可林j异源生产的方法及应用

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US20040077069A1 (en) * 2000-12-13 2004-04-22 Van De Laar Antonius Johannes Method for the production of a heterologous protein by a fungus
US7927863B2 (en) * 2003-10-22 2011-04-19 Alder Biopharmaceuticals, Inc. Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy

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US7927863B2 (en) * 2003-10-22 2011-04-19 Alder Biopharmaceuticals, Inc. Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy

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Also Published As

Publication number Publication date
EP2710114B1 (fr) 2021-11-03
MX368546B (es) 2019-10-07
EP2710114A4 (fr) 2014-11-19
CN103827290A (zh) 2014-05-28
ES2899381T3 (es) 2022-03-11
EA028913B1 (ru) 2018-01-31
EA201301294A1 (ru) 2014-05-30
IL229430A0 (en) 2014-01-30
KR20140033445A (ko) 2014-03-18
JP2018007656A (ja) 2018-01-18
HUE060418T2 (hu) 2023-02-28
RS62628B1 (sr) 2021-12-31
NZ718187A (en) 2017-10-27
EA028913B9 (ru) 2018-03-30
IL229430B (en) 2020-07-30
PT2710114T (pt) 2021-12-09
LT2710114T (lt) 2021-12-10
KR101981662B1 (ko) 2019-05-23
JP6193220B2 (ja) 2017-09-06
CA2837000A1 (fr) 2012-11-29
SI2710114T1 (sl) 2022-01-31
DK2710114T3 (da) 2021-12-06
CY1124796T1 (el) 2022-11-25
WO2012161956A2 (fr) 2012-11-29
CN110408644A (zh) 2019-11-05
NZ618635A (en) 2016-09-30
EP2710114A2 (fr) 2014-03-26
SG194937A1 (en) 2013-12-30
MX2019012022A (es) 2019-11-18
MX2013013532A (es) 2015-08-20
CA2837000C (fr) 2021-09-14
WO2012161956A9 (fr) 2013-09-06
PL2710114T3 (pl) 2022-01-17
HRP20211873T1 (hr) 2022-03-04
JP2014517698A (ja) 2014-07-24
JP6491696B2 (ja) 2019-03-27
BR112013029902A2 (pt) 2016-12-20

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