WO2012095200A1 - Cartouche de réactifs, puce de traitement, kit d'analyse les comportant et procédé d'analyse - Google Patents

Cartouche de réactifs, puce de traitement, kit d'analyse les comportant et procédé d'analyse Download PDF

Info

Publication number
WO2012095200A1
WO2012095200A1 PCT/EP2011/069996 EP2011069996W WO2012095200A1 WO 2012095200 A1 WO2012095200 A1 WO 2012095200A1 EP 2011069996 W EP2011069996 W EP 2011069996W WO 2012095200 A1 WO2012095200 A1 WO 2012095200A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent cartridge
processing chip
channels
reagent
cartridge
Prior art date
Application number
PCT/EP2011/069996
Other languages
German (de)
English (en)
Inventor
Jochen Rupp
Martina Daub
Original Assignee
Robert Bosch Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch Gmbh filed Critical Robert Bosch Gmbh
Publication of WO2012095200A1 publication Critical patent/WO2012095200A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber

Definitions

  • liquids are passed through channels and reaction chambers.
  • These systems usually consist of a device for the control, a replaceable processing chip and a disposable chip with reagents. Device and chip together form the platform.
  • EP 1415788 describes a processing chip with a microfluidic system and a field of upstream samples.
  • a trend in so-called lab-on-chip systems for diagnostics and analytics is the development of flexible platforms or devices with a processing chip, which are used in conjunction with disposable cartridges, which are disposed of after a single use. These cartridges often contain microfluidic channels, reaction chambers, and other functions to perform the required on-chip biochemical operations.
  • Waste container for collecting and storing the leaked liquids The hoses and containers are often in spite of careful treatment in a short time of
  • Germs, fungi and algae Germs, fungi and algae.
  • Multi-step biochemical reactions require the observance of special sequences of fluid additions and withdrawals, so-called biochemical effluent protocols; For example, lysis buffer, binding buffer, washing buffer 1, washing buffer 2, elution buffer in a specific sequence must be defined in succession on a filter in a reaction chamber Volumes are pipetted and discarded elsewhere.
  • protocols are often offered as so-called kits along with all the disposable articles needed, eg, DNA purification columns, plus all the reagents needed. Different applications are addressed by different kits.
  • cooperating components optionally both preformed in chip form, namely a processing chip and a reagent cartridge. These two components are connected together shortly before or during use. This avoids the storage of large amounts of liquid in or on the device and the associated sterility problems including a subsequent falsification of the analysis results.
  • the reagents are pre-stored in a reagent cartridge which can be docked to the processing chip shortly before or during use. This improves the storage, shelf life, chemical stability and sterility of analysis kits, responsive liquid volumes, and avoidance of
  • Urine sample analysis other biochemical protocols than a blood sample analysis.
  • Reagent cartridge greatly simplifies handling of the application and places fewer demands on the platform operator.
  • the simplified application allows safe handling and reduces operator error.
  • the principle of cooperating processing chip and reagent cartridge allows the
  • the reagent cartridge is not limited in volume, in contrast to the most flat processing chip.
  • the separate deployment results in an enormous flexibility in layout and design as well as in size the storable volumes. Evaporation is the concept in this concept
  • Reagent pre-storage no longer a problem.
  • An analysis kit system consists of two components, for example.
  • a component includes at least the fluidic structures and functions and provides reaction chambers and channels. The second component is only shortly before
  • the platform takes over activation to track the biochemical protocols.
  • the connection technology and interfaces to the platform are selected in such a way that maximum flexibility of the processes and the
  • the reagent cartridge can also be designed as a reagent chip.
  • FIG. 1 is a schematic plan view of a reagent cartridge and a processing chip prior to connection according to an embodiment of the present invention.
  • FIG. Fig. 2 shows a schematic side view of the reagent cartridge and the
  • FIG. 3 shows a schematic plan view of the reagent cartridge and the processing chip from FIG. 1 after their connection according to an embodiment of the present invention.
  • Fig. 4 shows a schematic side view of the reagent cartridge
  • FIG. 5 shows a flowchart of the method according to an embodiment of the present invention.
  • FIGS. 1 to 4 each schematically show the same reagent cartridge 10 and the same processing chip 30 on the one hand before and on the other hand after
  • the reagent cartridge 10 has in a cartridge housing 11 two small storage chambers 12 and two large storage chambers 13 with four different reagents.
  • the storage chambers 12, 13 are arranged in a cartridge body 14 and are closed by a film 15.
  • the film 15 is welded to storage chambers 12, 13 flat with the cartridge body 14 and forms seals of the storage chambers 12, 13.
  • the film 15 separates the cartridge body with the storage chambers 12, 13 of a cartridge upper part 16.
  • the cartridge upper part 16 has a first fluidic structure 17th with adjacent to the film 15 first channels 18.
  • the reagent cartridge 10 has in
  • Cartridge top 16 further includes a receiving portion 19 for receiving a portion of the processing chip 30.
  • the first channels 18 terminate with first openings 20 at defined positions of the receiving portion 19.
  • the reagent cartridge 10 further has an opening mechanism 21 for opening the seal of the storage chamber 12, 13 in the cartridge upper part 16.
  • the opening mechanism 21 is shown here with a contact lever 22 and lancets 23.
  • the reagent cartridge 10 also has a stop 24 and a first one in the receiving portion 19
  • the processing chip 30 has a second fluidic structure 31 with second channels 32, which are only partially shown.
  • the processing chip 30 includes in the second fluidic structure 31 other application-specific elements, not shown, such as
  • the second channels 32 terminate with second openings 33 at defined positions of an insertion section 34.
  • the insertion section 34 is configured to engage with the receiving section 19 of FIG.
  • the insertion section 34 has for this purpose a leading edge 35 and a second latching section 36 with lateral
  • Figs. 1 and 2 show the reagent cartridge 10 and the processing chip 30 before and the merging.
  • Processing chip 30 is made by moving the processing chip 30 in the direction of the arrows
  • Reagent cartridge 10 and processing chip 30 is completed when both are in a latched state.
  • Figs. 3 and 4 show the reagent cartridge 10 and the processing chip 30 after merging in the latched state.
  • the leading edge 35 of the insertion section 34 on the processing chip 30 touches the stop 24 in the receiving section 19 of the reagent cartridge 10 and the latching of the first latching section 26 with the second latching section 36 has taken place.
  • this is due to the position of the lateral tongues 27 of the receiving portion in the
  • Recess recesses 37 of the insertion section 34 to shown.
  • the locking is irreversible.
  • Reagent cartridge 10 and processing chip 30 are now positioned in a defined manner to each other and the openings 20 of the receiving portion 19 are each at the associated by means of defined positions openings 33 of the insertion section 34. Furthermore, there is now a fluidic connection of the
  • This connection is made fluidly tight.
  • Processing chip 30 by means of a control device, not shown in this example by compressing the pantries.
  • Locking sections 26, 36 and the cooperating openings 20, 33 each form a mechanical fluidic interface 40 on the reagent cartridge 10 and on the processing chip 30.
  • the reagent cartridge 10 and the processing chip 30 are coupled by means of the mechanical fluidic interface 40.
  • Opening mechanism designed such that it externally driven by the
  • Control unit is controlled. This allows a sequential opening of the seals of the pantries.
  • Alternative embodiments, not shown, of the opening mechanism for opening the seal by piercing the foil with lancets are opening by means of twisting off or breaking off at a predetermined breaking point or by pulling off a foil.
  • the reagent cartridge 10 is preferably filled in the preparation via the openings 12, 13 with at least one reagent and then sealed.
  • the reagent cartridge can be prefabricated empty and the filling is carried out later by another, not shown, filling opening to each pantry.
  • An analysis kit with a reagent cartridge and a processing chip forms a complete unit of consumables for analysis.
  • a kit additionally has a protocol.
  • the invention and the assay kit may also include multiple reagent cartridges and / or multiple processing chips. Then the interfaces and
  • FIG. 5 shows a flowchart 42 of the analysis method according to an embodiment of the present invention.
  • the analysis method uses a reagent cartridge and a processing chip, wherein the reagent cartridge has at least one
  • the analysis method begins with method step a. to c. in any order:
  • the method steps b. to d. controlled by a controller according to an assay protocol.
  • the controller takes one
  • Quantity control of the reagents The delivery of the reagents is accomplished by compressing the reservoir containing the reagent.
  • the opening of the seals of various reservoir chambers of the reagent cartridge may be simultaneous or sequential according to the assay protocol.
  • the type of fluidic contacting can be done in many ways. For example, If lancets click together, they could puncture a membrane and thus establish the fluidic connection. Sealings of the reagent chip may be withdrawn, twisted off or broken off like a foil to make a subsequent fluidly sealed connection with the processing chip.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne une cartouche de réactifs (10) et une puce de traitement (30) qui sont couplées au moyen d'une interface fluide mécanique (40). La cartouche de réactifs (10), ayant au moins une chambre de stockage (12, 13) pour au moins un réactif et ayant des premiers canaux (18), comporte une interface fluide mécanique (40) ayant une première section d'enclenchement mécanique (26) pour l'enclenchement avec une puce de traitement (30). La puce de traitement (30) présente une seconde section d'enclenchement mécanique (36) et des seconds canaux (32). Les premiers canaux (18) et les seconds canaux (32) mutuellement associés sont liés fluidiquement entre eux dans un état enclanché.
PCT/EP2011/069996 2011-01-12 2011-11-14 Cartouche de réactifs, puce de traitement, kit d'analyse les comportant et procédé d'analyse WO2012095200A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011002571.5 2011-01-12
DE201110002571 DE102011002571A1 (de) 2011-01-12 2011-01-12 Reagenzienkartusche, Prozessier-Chip, Analyse-Kit damit und Analyseverfahren

Publications (1)

Publication Number Publication Date
WO2012095200A1 true WO2012095200A1 (fr) 2012-07-19

Family

ID=45218662

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/069996 WO2012095200A1 (fr) 2011-01-12 2011-11-14 Cartouche de réactifs, puce de traitement, kit d'analyse les comportant et procédé d'analyse

Country Status (2)

Country Link
DE (1) DE102011002571A1 (fr)
WO (1) WO2012095200A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2962758B1 (fr) 2014-07-01 2017-07-19 ThinXXS Microtechnology AG Cellule d'écoulement dotée d'une zone de stockage et d'un canal de transport pouvant s'ouvrir à un point de rupture
WO2020035767A1 (fr) * 2018-08-13 2020-02-20 Beckman Coulter Inc. Instrument de laboratoire pour tester un échantillon de patient

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1415788A1 (fr) 2002-10-31 2004-05-06 Agilent Technologies, Inc. Dispositif de reseau microfluidique integre
DE102006034196A1 (de) * 2005-07-29 2007-02-08 Hitachi High-Technologies Corp. Chemische Analysevorrichtung und chemische Analysepatrone
WO2008141437A1 (fr) * 2007-05-18 2008-11-27 Axela Inc. Dispositif pour réaction chimique ayant des éléments optiques et de régulation de fluide intégrés
WO2010091246A2 (fr) * 2009-02-06 2010-08-12 Northwestern University Conditionnement de liquide pouvant éclater et ses utilisations

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1415788A1 (fr) 2002-10-31 2004-05-06 Agilent Technologies, Inc. Dispositif de reseau microfluidique integre
DE102006034196A1 (de) * 2005-07-29 2007-02-08 Hitachi High-Technologies Corp. Chemische Analysevorrichtung und chemische Analysepatrone
WO2008141437A1 (fr) * 2007-05-18 2008-11-27 Axela Inc. Dispositif pour réaction chimique ayant des éléments optiques et de régulation de fluide intégrés
WO2010091246A2 (fr) * 2009-02-06 2010-08-12 Northwestern University Conditionnement de liquide pouvant éclater et ses utilisations

Also Published As

Publication number Publication date
DE102011002571A1 (de) 2012-07-12

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