EP2535108B1 - Système microfluidique et son procédé de d'utilisation - Google Patents

Système microfluidique et son procédé de d'utilisation Download PDF

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Publication number
EP2535108B1
EP2535108B1 EP12163529.6A EP12163529A EP2535108B1 EP 2535108 B1 EP2535108 B1 EP 2535108B1 EP 12163529 A EP12163529 A EP 12163529A EP 2535108 B1 EP2535108 B1 EP 2535108B1
Authority
EP
European Patent Office
Prior art keywords
drum
chamber
pressure
actuator
adapter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP12163529.6A
Other languages
German (de)
English (en)
Other versions
EP2535108A1 (fr
Inventor
Martina Daub
Juergen Steigert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
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Filing date
Publication date
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Publication of EP2535108A1 publication Critical patent/EP2535108A1/fr
Application granted granted Critical
Publication of EP2535108B1 publication Critical patent/EP2535108B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0841—Drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/598—With repair, tapping, assembly, or disassembly means

Definitions

  • biochemical processes are based in particular on the handling of liquids. Typically, this manipulation is done manually with tools such as pipettes, reaction vessels, active probe surfaces or laboratory equipment. By pipetting robots or special equipment, these processes are already partially automated.
  • Microfluidic systems are sometimes referred to as so-called lab-on-a-chip systems (Westentaschenlabor or chip laboratory), which accommodate the entire functionality of a macroscopic laboratory on a plastic plastic card sized plastic substrate.
  • Lab-on-a-chip systems typically consist of two major components.
  • a test carrier or disposable cartridge includes structures and mechanisms for the implementation of basic fluidic operations (e.g., mixers) which may consist of passive components such as channels, reaction chamber, upstream reagents, or even active components such as valves or pumps.
  • the second main component is actuation, detection and control units.
  • Such a lab-on-a-chip system is for example in the document DE 10 2006 003 532 A1 described.
  • This system comprises a rotor chip, which is rotatably provided with respect to a stator chip.
  • the rotor chip can be coupled by means of fluidic channels with the stator chip for filling or emptying the rotor chip.
  • WO 02/20160 A1 discloses a fluidic assay device having a carousel rotatably mounted on a hub, the carousel having a plurality of respective paired opposed chambers which may be fluidly contacted in pairs via a passageway in the hub.
  • the document US 5,573,951 discloses a multi-chamber device for simultaneously performing two blood tests of a blood sample, wherein an integrated unit with a rotatable blood input valve is provided.
  • the system defined in claim 1 and the method defined in claim 15 have the advantage over conventional solutions that the cartridge is not centrifuged in a centrifuge or another force field must be suspended in order to transfer the component between the first and second chamber.
  • many parameters, such as the temperature of the component can be adjusted more easily in a stationary system.
  • a more flexible processing of the component is possible because the processing is independent of the speed of the centrifuge.
  • Component in this case means a liquid, a gas or a particle.
  • chamber is presently preferably a line section, which is designed to be open on both sides or only one side open, or a substantially closed space meant, which has an inlet and / or outlet.
  • the pressure device is designed as a pump and / or pressure accumulator, wherein the pump and / or the accumulator is preferably connected by means of a pressure connection with the cartridge or integrated into the cartridge. This will simply provide the necessary pressure to transfer the component between the first and second chambers.
  • a very compact construction can be achieved by means of the integration.
  • the "pressure" can be an overpressure or a negative pressure relative to the ambient pressure.
  • the pressure accumulator stores the component itself under pressure and the first or second chamber supplies or stores a fluidic aid under pressure, which pressurizes the at least one component.
  • the pressure accumulator is designed in particular as a gas cartridge, bladder accumulator or spring accumulator.
  • the auxiliary is preferably a gas, in particular air, or water.
  • the cartridge has a housing, which is closed at its one end by means of an adapter, wherein the adapter has the pressure port.
  • the adapter has the pressure port.
  • several functions are integrated into the adapter: namely once a particular sterile closure of the housing and continue to receive the pressure port.
  • the pressure port can also be arranged at the end of the housing, which is opposite to the adapter.
  • the adjusting device comprises an electrically, mechanically and / or pressure-operated actuator which rotates the first drum and / or moves along the central axis.
  • the axial movement can therefore be provided in addition to the rotational movement and preferably takes place along the longitudinal axis of a housing of the cartridge.
  • the actuator has a shaft which is directly or indirectly connected to the first drum in order to rotate it. This allows the first drum to be rotated without having to rotate the other drums.
  • the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum, in order from a first position in which this is in a form-locking engagement with a housing of the cartridge in the rotational direction about the central axis in a second position along the central axis to spend, in which the positive connection is canceled and the first drum rotates about the central axis due to the action of a return means or another actuator.
  • a kind of "ballpoint pen mechanism" is provided.
  • the actuator actuates the first slope for cooperation with the second slope. That is, the actuator operates the ballpoint pen mechanism.
  • the first drum is a second and / or third drum upstream or downstream relative to the central axis, wherein the actuator actuates the second and / or third drum for rotating the first drum. That is, the actuator acts indirectly on the first drum to rotate it.
  • the cartridge has a housing which is closed at its one end by means of an adapter, wherein the actuator is attached to the adapter.
  • the actuator is integrated in the adapter.
  • the adapter has a flexible membrane which can be actuated on its one side by means of the actuator and acts on its other side on the first, second and / or third drum. This can create a more sterile outcome.
  • the actuator is preferably outside the interior of the housing.
  • the second chamber of the first drum is upstream or downstream relative to the central axis and formed in the second and / or third drum.
  • the second chamber and / or a third chamber of the first drum upstream or downstream relative to the central axis wherein preferably the first chamber by means of the adjusting either with the second chamber or the third chamber is conductively connected.
  • the mixing chamber can thus be the first drum forward and / or downstream or also provided in the first drum itself.
  • the mixing chamber can preferably be optionally connected to different further chambers as required.
  • a second drum which has the second chamber
  • a third drum which has the third chamber.
  • the second drum may also have the second chamber and the third chamber, for example.
  • a plurality of second chambers are provided which can be acted upon by the pressure device with a different pressure, preferably a respective second chamber means of a respective pressure port in the adapter with the pressure device or all the second chambers by means of a single pressure port in the Adapter are connected to the pressure device, wherein more preferably a respective second chamber is connected by means of a respective valve with the single pressure port.
  • the printing device drives the actuator.
  • the actuator drives the actuator.
  • FIG. 1 shows in a sectional view a cartridge 100 and schematically a printing device 101, which together form a system 103 according to an embodiment of the present invention.
  • a printing device 101 which together form a system 103 according to an embodiment of the present invention.
  • FIGS. 1 to 4E the structure of the cartridge 100 will be explained in more detail below.
  • the cartridge 100 includes a housing 102 in the form of a tube.
  • the housing 102 may be formed as a 5 to 100 mL, especially 50 mL, centrifuge tube, 1.5 mL or 2 mL Eppendorf tube, or alternatively as a microtiter plate (e.g., 20 ⁇ L per well).
  • the longitudinal axis of the housing 102 is designated 104.
  • a first drum 108, a second drum 106 and a third drum 110 are accommodated in the housing 102.
  • the drums 106, 108, 110 are arranged one behind the other and with their respective central axes coaxial with the longitudinal axis 104.
  • the housing 102 is formed closed at its one end 112. Between the closed end 112 and the adjacent to this third drum 110, a return means, for example in the form of a spring 114 is arranged.
  • the spring 114 may be in the form of a coil spring or a polymer, in particular an elastomer.
  • the other end 116 of the housing 102 is closed by means of a closure 118.
  • the closure 118 may be removed to remove the drums 106, 108, 110 from the housing 102.
  • the housing 102 itself can be dismantled to remove the drums 106, 108, 110 or to reach the chambers, for example the chamber 136.
  • the spring 114 is disposed between the shutter 118 and the second drum 106, so that the spring 114 is stretched to generate a restoring force.
  • Other arrangements of the spring 114 are conceivable.
  • the first drum 108 connected downstream of the second drum 106 comprises a mixing chamber 124 in which the reagents from the chambers 120 are mixed with the sample from the chamber 122.
  • the second drum 108 includes, for example, another chamber 126 in which the mixture 128 flows from the mixing chamber 124 through a solid phase 130.
  • the solid phase 130 may be a gel column, a silica matrix, or a filter.
  • the third drum 110 which is in turn connected downstream of the first drum 108, comprises a chamber 132 for receiving a waste product 134 from the chamber 126. Furthermore, the third drum 110 comprises a further chamber 136 for receiving the desired end product 138.
  • the mixing chamber 124 is first to be fluidly connected to the chamber 122 to receive the sample from the chamber 122. Thereafter, the mixing chamber 124 is to be connected to the chambers 120 to receive the reagents from these. Subsequently, the reagents and the sample are to be mixed in the mixing chamber 124.
  • the processes in chambers 126, 132 and 136 should also be similar.
  • FIG. 2A-2G perspective view of various components of the cartridge 100 from FIG. 1 , Based on Figures 2A-2G in particular an adjusting device 300 (see Fig. 3A ) comprising the actuator 139, which enables the control of the above-mentioned processes.
  • the housing 102 on its inside projections 200.
  • the projections 200 are radially from the housing inner wall 202 toward the longitudinal axis 104.
  • the projections 200 form between them slots 204 which extend along the longitudinal axis 104.
  • the projections 200 are at their one end each formed with a slope 206.
  • the ramps 206 face in a first direction 207. According to the present embodiment, they point toward the end 112 of the housing 102.
  • FIG. 2B shows the end 112 of the housing 102, which is formed according to this embodiment as a removable cap.
  • the end 112 has at its inner periphery a plurality of grooves 208 which extend along the longitudinal axis 104.
  • FIG. 2C shows the second drum 106 with the chambers 120, 122.
  • the drum 106 has on its outer wall 210 a plurality of projections 212 which extend from the outer wall 210 radially outwardly.
  • the projections 212 of the drum 106 engage in the slots 204 of the housing 102.
  • a rotation of the drum 106 is locked about the longitudinal axis 104.
  • the drum 106 is slidable along the longitudinal axis 104 in the slots 204.
  • the second drum 106 furthermore has on its outer wall 210, in particular on its end 214 facing the first drum 108, a crown-like contour 216 which comprises a multiplicity of bevels 218, 220.
  • Two bevels 218, 220 each form a point of the crown-like contour 216.
  • the slopes 218, 220 also point in the first direction 207.
  • FIG. 2D shows a view of the second drum 106 from Figure 2C from underneath.
  • the underside 222 of the second drum 106 assigned to the end 214 has a plurality of openings 224 in order to connect the chambers 120, 122 to the mixing chamber 124 of the first drum 108 in liquid, gas and / or particle form (hereinafter "conductive").
  • the openings 224 may also conductively connect the chambers 120, 122 to the chamber 126 of the first drum 108.
  • a respective conductive connection is determined by the position of a respective opening 224 with respect to the chambers 124, 126. This position is achieved by rotating the first drum 108 relative to the second drum 106, as will be explained in more detail later.
  • FIG. 2E shows a lancing device 226, which in FIG. 1 not shown.
  • Lancing device 226 includes a plate 228 having one or more spikes 230 disposed adjacent to an opening 232 in plate 228, respectively.
  • the mandrels 230 serve, by means of suitable control by the actuator 139 a respective Opening 224 in the bottom 222 of the second drum 106 to pierce, whereupon in particular liquid from the corresponding chamber 120, 122 flows through the opening 232 into the chambers 124 or 126.
  • Figure 2F shows the first drum 108 with the chambers 124, 126.
  • an opening 236 for a conductive connection of the chamber 126 with the chambers 132, 136 of the third drum 110 is provided.
  • the first drum 108 has a plurality of projections 240 on its outer wall 238.
  • the protrusions 240 are configured to engage the slots 204 (as well as the protrusions 212 of the second drum 106). As long as the projections 240 are engaged with the slots 204, rotation of the first drum 108 about the longitudinal axis 104 is disabled. However, the projections 240 along with the first drum 108 are movable along the longitudinal axis 104 in the slots 204.
  • the protrusions 240 have bevels 242 which are opposite to the first direction in a second direction 243 and are formed corresponding to the slopes 206 and 220. According to the present exemplary embodiment, the second direction 243 points in the direction of the closure 118.
  • FIG. 2G shows the third drum 110 with the chambers 132, 136.
  • the drum 110 has projections 244 which project from the outer wall 246 of the drum 110 respectively.
  • the projections 244 are adapted to engage the grooves 208 of the end 112 so that the drum 110 is displaceable in the longitudinal direction 104 in the grooves 208. A rotation of the drum 110 about the longitudinal axis 104 is thus locked.
  • FIG. 3A-3E show several operating conditions during operation of the cartridge 100 FIG. 1 , wherein an additional drum 302 is shown, but this is not relevant in the present case.
  • the Figures 4A-4E correspond respectively with the Figures 3A-3E and illustrate the movement of the ramps 206, 218, 220, 242 relative to each other.
  • FIG. 3B shows an operating state of the cartridge 100 which is more advanced than that in FIG FIG. 4B shown condition.
  • the housing 102 is shown partially transparent to reveal the interior.
  • FIGS. 3A and 4A show a first position in which the projections 240 of the first drum 108 engage in the slots 204 and thus a rotation of the first drum 108 is locked about the longitudinal axis 104.
  • the actuator 139 now presses indirectly or directly on the second drum 106, the second drum 106 in turn pushes the bevels 242 of the first drum 108 against the action of the spring 114 by means of the bevels 220 of the contour 216, the spring 114 being compressed.
  • the first drum 108 moves in the first direction 207 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
  • the spring 114 moves the first drum 108 again in the second direction 243 by means of the third drum 110.
  • the second drum 106 together with its bevels 220 is also moved again in the second direction 243 whereby the chamfers 242 of the first drum 108 come to rest against the chamfers 206 of the housing 102 and slide therealong into a third position, as in Figs Figures 4D and 4E shown.
  • the projections 240 of the first drum 108 are again located in the slots 204 of the housing 102, so that further rotation of the first drum 108 about the longitudinal axis 104 is again locked.
  • the process described above may be repeated as many times as desired to rotate the first drum 108 in a defined manner relative to the other drums 106 and 110.
  • the cartridge 100 may have an outer geometry, so that these in a receptacle of a rotor of the centrifuge, in particular in a receptacle of a Ausschwingrotors or fixed angle rotor of a centrifuge, can be used.
  • the cartridge 100 is moved by one in FIG. 1 schematically indicated pivot point 140 rotated at high speed.
  • the pivot point 140 lies on the longitudinal axis 104, so that a corresponding centrifugal force 142 along the longitudinal axis 104 acts on each component of the cartridge 100.
  • suitable control of the rotational speed various processes within the cartridge 100 can be controlled, as with the use of the actuator 139.
  • the actuator 139 may be electrically, mechanically and / or pressure-operated.
  • a piezoelectrically, electrostatically, semi-mechanically manually or electromagnetically operated actuator 139 is suitable.
  • “Operated” here means the active principle which the actuator 139 exploits in order to generate the actuating force for actuating the second drum 106 (or, depending on the embodiment, also one of the other drums 108, 110).
  • the actuator 139 may include an electromagnet that cooperates with a metal part disposed in one of the drums 106, 108, 110 that the electromagnet attracts or repels by appropriately driving it to thereby effect the above-described adjustment of the drums 106, 108, 110 to reach each other.
  • the compressive force applied to the second drum 106 by means of the actuator 139 is typically 0.5-100 N.
  • a suitable, not shown, control device which controls the actuator 139, so that the drums 106, 108, 110 at the desired time to take the respective desired position to each other.
  • the control device may have a timer and / or an integrated circuit.
  • the system 103 may be provided without the protrusions 200, the slots 204, the slopes 206, the protrusions 212, the slopes 218, 220 and return spring 114.
  • the actuator 139 has a shaft which is directly connected to the first drum 108. The actuator 139 then rotates under suitable control by means of the control device, the first drum 108 with respect to the then fixed other drums 106, 110 in order to conductively connect the various chambers, for example the chambers 120, 124 with each other.
  • two or more actuators 139 can be used.
  • FIG. 5 schematically shows in a sectional view a system 103 according to another embodiment of the present invention.
  • the lid 118 is in the form of an adapter for holding the actuator 139 is executed.
  • the actuator 139 extends through the adapter 118 and thus acts directly on the second drum 106 to these in the first direction 207, ie in Fig. 5 down, to move.
  • the actuator 139 for this purpose, an actuator, in particular a rod having, which presses against the drum 106.
  • the provision can be made as described above by means of the return means 114.
  • the actuator 139 for example, the actuator, be firmly connected to the second drum 106.
  • the drum 106 can be rapidly reciprocated along the longitudinal direction 104 by means of the actuator 139, whereby a mixing chamber for mixing components in one of the chambers 120, 122 could be provided. If the amplitude of the to-and-fro motion is chosen to be sufficiently small, this movement can occur without twisting the drums 106, 108, 110 relative to each other, i. the "ballpoint pen mechanism" is not triggered.
  • the pressure device 101 has the function of applying at least one component 500, in particular a liquid, for example a reagent, with a pressure difference in order to transfer it, for example, from the chamber 120 into the chamber 124.
  • the chambers 120, 124 are first arranged opposite each other (by means of rotating the first drum 108, as described above) and thereafter pressure-tightly connected to each other.
  • the second drum 106 seals against the adapter 118 so that a corresponding channel in the adapter 118 carrying the pressure is pressure-tightly connected to the chamber 120.
  • the pressure device 101 then applies, for example, a pressure which is above the ambient pressure to the adapter-side end 502 of the chamber 120.
  • the chamber 124 is vented to the atmosphere so that the pressure drives the component into the chamber 124.
  • the chamber 124 in turn with other chambers 126, 132, 136 (see FIG. 1 ) in the first drum 108 and / or in the third drum 110 to be conductively connected, wherein only the last chamber 136 is vented, so that the pressure of the component 500 or a mixture of the component 500 with other components or only a component of the component 500 by the chambers 124, 132, 136 drives.
  • the pressure device 101 typically generates a pressure of 0.01-2 bar.
  • the pressure device 101 may also be provided to provide the pressure difference by generating a vacuum.
  • the printing device 101 is designed, for example, as a pump.
  • a pump for example, it can be a hand-operated or electrically operated pump.
  • the printing device 101 may be formed as a pressure accumulator.
  • the pressure accumulator 101 can be designed, for example, as a spring accumulator, which initially contains the component 500 itself and, in particular, conveys the component 500 through the chamber 120 into the chamber 124 by actuation of a valve.
  • the printing device 101 stores a fluidic aid under pressure. As an aid especially compressed air comes into question. Relaxing the air, this drives the component 500, in particular a liquid, from the chamber 120 into the chamber 124 or through a plurality of chambers, as described above.
  • the printing device 101 is provided in particular outside the cartridge 100 and connected to the cartridge 100, in particular the adapter 118, for example by means of a pressure port 504.
  • the pressure device 101 in particular in the form of a compressed gas storage, could also be integrated in the cartridge 100, in particular in one of the chambers 120, 122, 124, 126, 132, 136.
  • FIG. 6 schematically shows in a sectional view a system 103 according to yet another embodiment of the present invention.
  • the embodiment according to FIG. 6 differs from the according to FIG. 5 in that the actuator 139 is attached to the outside of the adapter 118, ie, the actuator 139 does not penetrate the adapter 118 in this case. Rather, the actuator 139 acts indirectly, for example by means of a flexible membrane, on the second drum 106 to operate them in the first direction 207. In particular, forms thin portion 600 of the adapter 118 from the membrane, wherein an actuator 602 of the actuator 139 elastically deforms this thin portion 600.
  • FIG. 7 schematically shows in a sectional view a system 103 according to yet another embodiment of the present invention.
  • the embodiment according to FIG. 7 differs from the according to FIG. 5 in that a respective chamber 120, 122 of the second drum 106 is connected to a respective pressure device 101 by means of a respective associated pressure port 504. As a result, the pressures applied to the chambers 120, 122 can be controlled individually.
  • the adapter 118 has a connector (not shown), e.g. the housing 102, the second drum 106 and / or the chambers 120, 122 are contacted and sealed.
  • the plug-in device may have pins (not shown), which engage in the chambers 120, 122 or other openings of the drum 106 from above and seal them pressure-tight.
  • the pins can also open when mating the example, previously closed chambers 120, 122 or other openings, in particular piercing a cover.
  • a channel connected to a pressure port 504 which opens into an associated chamber 120, 122, can run in a respective journal.
  • FIG. 8 schematically shows in a sectional view a system 103 according to yet another embodiment of the present invention.
  • the embodiment according to FIG. 8 differs from the according to FIG. 7 in that a respective chamber 120, 122 in the second drum 106 are connected by means of a valve 700 to a single pressure port 504.
  • the valves 700 may be integrated into the adapter 118.
  • one or more spikes 802 can be provided on the inside 800 (that is to say, facing the interior of the housing 102).
  • the actuator 139 Prior to the pressure drop compensation by means of the pressure device 101, the actuator 139 first actuates the second drum 106 in the second direction 243 in order thereby to seal a cover film (not shown) which closes off a respective chamber 120, 122.
  • a cover film for example, made of aluminum, to pierce.
  • the drums 106, 108, 110 are appropriately rotated relative to one another, as explained above.
  • the spines 802 may be provided extendable. This is then a piercing of a respective cover regardless of the operation by the actuator 139 is possible.
  • the actuator 139 pressure-operated, to which the actuator 139 is pressure-conductively connected to the pressure device 101 (not shown) and thus driven by the latter.
  • the adapter 118 and the second drum 106 form a chamber (not shown) with each other, which is acted upon by pressure from the pressure device 101 and thus the actuator 139 is formed.
  • the actuator 139 could be provided in the form of a bellows, which is provided between the adapter 118 and the second drum 106.
  • the actuator 139 may also be provided elsewhere, for example between the first drum 108 and the second or third drum 106, 110.
  • the actuator 139 may also be omitted, in which case the rotation of the drums 106, 108, 110 to one another takes place manually, in particular by triggering the ballpoint pen mechanism.
  • a control unit controls the interaction of the actuator 139, which determines the spatial positioning of the drums 106, 108, 110 and the printing device 101, which controls the pressure for controlling the component 500 (or more components).
  • drums 106, 108, 110 and the chambers 120, 122, 124, 132, 136, respectively, can be designed so that further process steps and structures can be integrated, e.g. Sedimentation structures, mixed structures, channel or siphon structures for the forwarding and switching of liquids.
  • the housing 102 and the drums 106, 108, 110 may be made of the same or different polymers.
  • the one or more polymers are, in particular, thermoplastics, elastomers or thermoplastic elastomers. Examples are cycloolefin polymer (COP), cyclo-olefin copolymer (COC), Polycarbonates (PC), polyamides (PA), polyurethanes (PU), polypropylene (PP), polyethylene terephthalate (PET) or poly (methyl methacrylate) (PMMA).
  • COP cycloolefin polymer
  • COC cyclo-olefin copolymer
  • PC polyamides
  • PA polyurethanes
  • PU polypropylene
  • PET polyethylene terephthalate
  • PMMA poly (methyl methacrylate)
  • One or both drums 106, 110 may be formed integrally with the housing 102.

Claims (14)

  1. Système microfluidique (103), présentant :
    une cartouche (100) avec un premier tambour (108) qui présente une première chambre (124) et avec un dispositif de réglage (300) qui est prévu pour faire tourner le premier tambour (108) autour de son axe médian (104), afin de relier de ce fait la première chambre (124) à une deuxième chambre (120, 122, 132, 136) de manière à conduire les fluides et/ou les gaz et/ou les particules, et
    un dispositif de pression (101) qui sollicite au moins un composant (500) avec une différence de pression afin de transférer celui-ci de ce fait entre la première chambre (124) et la deuxième chambre (120, 122, 132, 136), caractérisé en ce que la deuxième chambre (120, 122, 132, 136) est montée en amont ou en aval du premier tambour (108) par rapport à l'axe médian (104), et est réalisée dans un deuxième ou un troisième tambour (106, 110).
  2. Système selon la revendication 1, dans lequel le dispositif de pression (101) est réalisé sous forme de pompe et/ou d'accumulateur de pression, la pompe et/ou l'accumulateur de pression étant de préférence connectés au moyen d'un raccord de pression (504) à la cartouche (100) ou étant intégrés dans la cartouche (100).
  3. Système selon la revendication 2, dans lequel l'accumulateur de pression (101) stocke le composant (500) lui-même sous pression et l'achemine à la première chambre (124) ou à la deuxième chambre (120, 122, 132, 136) ou stocke un auxiliaire fluidique sous pression, qui met sous pression l'au moins un composant (500).
  4. Système selon la revendication 2 ou 3, dans lequel la cartouche (100) présente un boîtier (102) qui est fermé au niveau de l'une de ses extrémités au moyen d'un adaptateur (118), l'adaptateur (118) présentant le raccord de pression (504).
  5. Système selon l'une quelconque des revendications précédentes, dans lequel le dispositif de réglage (300) comprend un actionneur à commande électrique, mécanique et/ou par pression (139), qui fait tourner le premier tambour (108) et/ou le déplace le long de l'axe médian (104).
  6. Système selon la revendication 5, dans lequel l'actionneur (139) présente un arbre qui est connecté directement ou indirectement au premier tambour (108), afin de le faire tourner.
  7. Système selon la revendication 5, dans lequel le dispositif de réglage (300) comprend un premier biseau (220) qui coopère avec un deuxième biseau (242) du premier tambour (108), afin d'amener celui-ci d'une première position, dans laquelle il est en prise par engagement par coopération de formes avec un boîtier (102) de la cartouche (100) dans le sens de rotation autour de l'axe médian (104), dans une deuxième position le long de l'axe médian (104), dans laquelle l'engagement par correspondance de formes est supprimé et le premier tambour (108) tourne autour de l'axe médian (104) sous l'effet d'un moyen de rappel (114) ou d'un autre actionneur.
  8. Système selon la revendication 7, dans lequel l'actionneur (139) actionne le premier biseau (220) en vue de la coopération avec le deuxième biseau (242).
  9. Système selon l'une quelconque des revendications précédentes 5 à 8, dans lequel le deuxième tambour (106) et/ou le troisième tambour (110) sont montés en amont et/ou en aval du premier tambour (108) par rapport à l'axe médian (104), l'actionneur (139) actionnant le deuxième tambour (106) et/ou le troisième tambour (110) pour la rotation du premier tambour (108).
  10. Système selon l'une quelconque des revendications 5 à 9, dans lequel la cartouche (100) présente un boîtier (102) qui est fermé au niveau de l'une de ses extrémités au moyen d'un adaptateur (118), l'actionneur (139) étant fixé à l'adaptateur (118).
  11. Système selon la revendication 10, dans lequel l'adaptateur (118) présente une membrane flexible (600) qui peut être actionnée au niveau de l'un de ses côtés au moyen de l'actionneur (139) et qui agit au niveau de son autre côté sur le premier tambour (108), le deuxième tambour (106) et/ou le troisième tambour (110).
  12. Système selon l'une quelconque des revendications précédentes, dans lequel en plus de la première chambre (124) et de la deuxième chambre (120), au moins une chambre supplémentaire (122) est prévue, lesquelles chambres peuvent être sollicitées au moyen du dispositif de pression (101) avec une pression chaque fois différente les unes des autres, la deuxième chambre (120) et l'au moins une chambre supplémentaire (120, 122) étant de préférence à chaque fois connectées au moyen d'un raccord de pression respectif (504) dans l'adaptateur (118) au dispositif de pression (101) ou la deuxième chambre (120) et l'au moins une chambre supplémentaire (122) étant connectées au moyen d'un raccord de pression unique (504) dans l'adaptateur (118) au dispositif de pression (101), à chaque fois la deuxième chambre (120) et l'au moins une chambre supplémentaire (122) étant de préférence à chaque fois connectées au moyen d'une soupape respective (700) au raccord de pression unique (504).
  13. Système selon l'une quelconque des revendications 5 à 12, dans lequel le dispositif de pression (101) entraîne l'actionneur (139).
  14. Procédé pour faire fonctionner un système microfluidique (103) selon l'une quelconque des revendications précédentes, présentant les étapes suivantes :
    faire tourner un premier tambour (108) qui comprend une première chambre (124) autour de son axe médian (104) afin de connecter de ce fait la première chambre (124) à la deuxième chambre dans le deuxième ou le troisième tambour (106, 110) (120, 122, 132, 136) de manière à conduire les fluides et/ou les gaz et/ou les particules, et
    solliciter au moins un composant (500) avec une différence de pression afin de transférer celui-ci de ce fait entre la première chambre (124) et la deuxième chambre (120, 122, 132, 136).
EP12163529.6A 2011-06-07 2012-04-10 Système microfluidique et son procédé de d'utilisation Not-in-force EP2535108B1 (fr)

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DE102011077124A1 (de) 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren
DE102011077115A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren
DE102011077101A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Mikrofluidisches System und Verfahren zum Betreiben eines solchen Systems
DE102013220064B3 (de) * 2013-10-02 2014-12-24 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Vorrichtung und verfahren zum bewegen einer festphase in eine mehrzahl von kammern
DE102013222283B3 (de) 2013-11-04 2015-01-15 Robert Bosch Gmbh Vorrichtung und Verfahren zur Handhabung von Reagenzien
WO2016105508A2 (fr) * 2014-12-23 2016-06-30 California Institute Of Technology Dispositifs et procédés de mesures autonomes
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US20120312380A1 (en) 2012-12-13
DE102011077101A1 (de) 2012-12-13
EP2535108A1 (fr) 2012-12-19
CN102814203B (zh) 2016-02-10
CN102814203A (zh) 2012-12-12

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