EP2532427B1 - Cartouche, centrifugeuse et procédé - Google Patents

Cartouche, centrifugeuse et procédé Download PDF

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Publication number
EP2532427B1
EP2532427B1 EP12163525.4A EP12163525A EP2532427B1 EP 2532427 B1 EP2532427 B1 EP 2532427B1 EP 12163525 A EP12163525 A EP 12163525A EP 2532427 B1 EP2532427 B1 EP 2532427B1
Authority
EP
European Patent Office
Prior art keywords
drum
cartridge
chamber
switch
contact element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP12163525.4A
Other languages
German (de)
English (en)
Other versions
EP2532427A2 (fr
EP2532427A3 (fr
Inventor
Martina Daub
Juergen Steigert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2532427A2 publication Critical patent/EP2532427A2/fr
Publication of EP2532427A3 publication Critical patent/EP2532427A3/fr
Application granted granted Critical
Publication of EP2532427B1 publication Critical patent/EP2532427B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/15—Use of centrifuges for mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
    • B01F29/32—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor
    • B01F29/321—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor of test-tubes or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/42—Mixers with shaking, oscillating, or vibrating mechanisms with pendulum stirrers, i.e. with stirrers suspended so as to oscillate about fixed points or axes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/453—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements
    • B01F33/4533—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements supporting the stirring element in one point
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/22—Control or regulation
    • B01F35/2201—Control or regulation characterised by the type of control technique used
    • B01F35/2207—Use of data, i.e. barcodes, 3D codes or similar type of tagging information, as instruction or identification codes for controlling the computer programs, e.g. for manipulation, handling, production or compounding in mixing plants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/713—Feed mechanisms comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges
    • B01F35/7137—Piercing, perforating or melting membranes or closures which seal the compartments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/716—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components
    • B01F35/7161—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components the containers being connected coaxially before contacting the contents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/71725—Feed mechanisms characterised by the means for feeding the components to the mixer using centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0841—Drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • biochemical processes are based in particular on the handling of liquids. Typically, this manipulation is done manually with tools such as pipettes, reaction vessels, active probe surfaces or laboratory equipment. By pipetting robots or special equipment, these processes are already partially automated.
  • Lab-on-a-Chip systems (also referred to as the West Pocket Laboratory or the Chiplabor) accommodate all the functionality of a macroscopic laboratory on a plastic plastic-sized plastic substrate.
  • Lab-on-a-chip systems typically consist of two major components.
  • a test carrier includes structures and mechanisms for the implementation of basic fluidic operations (e.g., mixers), which may consist of passive components such as channels, reaction chambers and upstream reagents, or even active components such as valves or pumps.
  • the second main component is actuation, detection and control units.
  • Such systems make it possible to carry out biochemical processes fully automatically.
  • a lab-on-a-chip system is for example in the document DE 10 2006 003 532 A1 described.
  • This system comprises a rotor chip, which is rotatably provided with respect to a stator chip.
  • the rotor chip can be coupled by means of fluidic channels with the stator chip for filling or emptying the rotor chip.
  • EP 2 514 515 A1 is a cartridge with a first drum having a first chamber and with an adjusting device, which can rotate the first drum using the centrifugal force, thereby conductively connecting the first chamber to a second chamber known.
  • the cartridge defined in claim 1, the centrifuge defined in claim 14 and the method defined in claim 15 have the advantage over conventional solutions that an electrical switch is simply provided and actuated can be.
  • the switch can, in turn, be provided for switching a multiplicity of different devices, for example a heater or a sensor or semiconductor component.
  • Component in the present case means a liquid, a gas or one or more particles.
  • first and second component only two different states of the same substance may be meant:
  • the first component may be formed as a clumped portion and the second component as a liquid portion of the same substance.
  • the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum to the first drum from a first position in which the second bevel with a housing of the cartridge in the rotational direction about the central axis in a form-fitting engagement is to spend in a second position along the central axis and against the action of a return means in which the positive connection is released and the first drum rotates about the central axis.
  • This mechanism is also referred to as a "ballpoint pen mechanism" herein.
  • the first chamber preferably with different other chambers - as required - optionally be connected.
  • a second drum which has the second chamber, and / or a third drum is provided, which has the third chamber, wherein preferably the second drum of the first drum relative to the center axis upstream and / or third drum of the first drum is downstream.
  • a stack of, for example, three drums can be formed. However, more than three drums may be provided.
  • the switch comprises at least a first and a second contact element, which are contacted for the closed state of the switch and spaced apart for the open state of the switch, wherein the first contact element at an end face of the first drum and the second contact element is attached to one of the end face of the first drum facing end side of the second or third drum or the first contact element on the first, second or third drum and the second contact element on a housing of the cartridge, in particular on a projection thereof, is attached.
  • different switching concepts can be represented: First, the operation of the switch may depend on the position of the drums to each other, or the operation of the switch may depend on the position of a drum relative to the housing.
  • the switch comprises a plurality of first contact elements, which are selectively contactable by means of rotation of the first drum with the second contact element.
  • first contact elements which are selectively contactable by means of rotation of the first drum with the second contact element.
  • the first and second contact element in a contact position of the first drum, which adjoins the second position of the first drum, contacted each other, preferably in the contact position, the first drum and the housing, in particular projections thereof, each other engage behind to avoid an automatic adjustment of the first drum from its contact position to a third position due to the action of the return means.
  • the contact position of the first drum this is rotated relative to the second drum and has this but also approximated to the second drum in comparison to the second position.
  • the first and / or second contact element comprises at least one conductor track at least in sections and possibly at least one metallic bump.
  • Such contact elements are easy to produce.
  • the switch can be connected to a read-out device which is set up to read out a switching state of the switch and, if necessary, to store a switching curve of the switch.
  • this has a particular in or on a housing of the cartridge, the first, second and / or third drum provided heater for particular cyclic heating of the first, second and / or third chamber, which by means of operating the electrical Switch is switchable for heating.
  • the heating device can, for example, provide the necessary temperature profile in the first chamber so that a polymerase chain reaction can take place in a component in the first chamber.
  • the cartridge according to the invention further comprises a particular in or on a housing of the cartridge, the first, second and / or third drum provided semiconductor device, which is actuated by means of operating the electrical switch.
  • the semiconductor element may be, for example, a temperature sensor which is energized by the switch for performing a temperature measurement.
  • this is designed for insertion into a centrifuge and centrifuging thereof and the adjusting device is arranged to actuate the first drum for rotation about the central axis when the centrifugal force exceeds a predetermined threshold value and / or Adjusting an actuator, which actuates the first drum for rotating about the central axis directly or indirectly.
  • an actuator which actuates the first drum for rotating about the central axis directly or indirectly.
  • the force which moves the components for example liquids through the cartridge, can be provided as a centrifugal force.
  • there is a pressure device which generates a suitable pressure, in particular gas or liquid pressure, which moves the components through the cartridge.
  • the switch with a power source wired or wirelessly connected to the closed State of the switch to generate a current flow therethrough.
  • Wireless solutions can make use of coils or a battery in the cartridge.
  • FIG. 1 shows in a sectional view a cartridge 100 according to an embodiment of the present invention.
  • the cartridge 100 includes a housing 102 in the form of a tube.
  • the housing 102 may be formed as a 5 to 100 mL, especially 50 mL, centrifuge tube, 1.5 mL or 2 mL Eppendorf tube, or alternatively as a microtiter plate (e.g., 20 ⁇ L per well).
  • the longitudinal axis of the housing 102 is designated 104.
  • a first drum 108, a second drum 106 and a third drum 110 are accommodated in the housing 102.
  • the drums 106, 108, 110 are arranged one behind the other and with their respective central axes coaxial with the longitudinal axis 104.
  • the housing 102 is formed closed at its one end 112. Between the closed end 112 and the adjacent to this third drum 110, a return means, for example in the form of a spring 114 is arranged.
  • the spring 114 may be in the form of a coil spring or a polymer, in particular an elastomer.
  • the other end 116 of the housing 102 is closed by means of a closure 118.
  • the closure 118 may be removed to remove the drums 106, 108, 110 from the housing 102.
  • the housing 102 itself can be dismantled to remove the drums 106, 108, 110 or to reach the chambers, for example the chamber 136.
  • the spring 114 is disposed between the shutter 118 and the second drum 106, so that the spring 114 is stretched to generate a restoring force.
  • Other arrangements of the spring 114 are conceivable.
  • a respective drum 106, 108, 110 may have one or more chambers:
  • the second drum 106 includes a plurality of reagent chambers 120 and another chamber 122 for receiving a sample, such as a blood sample, taken from a patient.
  • a sample such as a blood sample
  • the first drum 108 connected downstream of the second drum 106 comprises a mixing chamber 124 in which the reagents from the chambers 120 are mixed with the sample from the chamber 122.
  • the drum 108 includes, for example, another chamber 126 in which the mixture 128 flows out of the mixing chamber 124 through a solid phase 130.
  • the solid phase 130 may be a gel column, a silica matrix, or a filter.
  • the third drum 110 which is in turn connected downstream of the first drum 108, comprises a chamber 132 for receiving a waste product 134 from the chamber 126. Furthermore, the drum 110 comprises a further chamber 136 for receiving the desired end product 138.
  • the cartridge 100 has an outer geometry so that it can be used in a receptacle of a rotor of a centrifuge, in particular in a receptacle of a swing-bucket rotor or fixed-angle rotor of a centrifuge.
  • the cartridge 100 is moved by one in FIG. 1 schematically indicated pivot point 140 rotated at high speed.
  • the pivot point 140 lies on the longitudinal axis 104, so that a corresponding centrifugal force 142 along the longitudinal axis 104 acts on each component of the cartridge 100.
  • the mixing chamber 124 is first to be fluidly connected to the chamber 122 to receive the sample from the chamber 122. Thereafter, the mixing chamber 124 is to be connected to the chambers 120 to receive the reagents from these. Subsequently, the reagents and the sample in the mixing chamber 124 are speed-controlled mixed. Similarly, the processes in the chambers 126, 132 and 136 are to be speed controlled.
  • FIG. 2A-2G perspective view of various components of the cartridge 100 from FIG. 1 , Based on Figures 2A-2G in particular an adjusting device 300 (see Fig. 3A ), which enables the speed-dependent control of the above-mentioned processes.
  • the housing 102 on its inside projections 200.
  • the projections 200 are radially from the housing inner wall 202 toward the longitudinal axis 104.
  • the projections 200 form between them slots 204 which extend along the longitudinal axis 104.
  • the projections 200 are formed at their one end in each case with a slope 206.
  • the slopes 206 face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
  • FIG. 2B shows the end 112 of the housing 102, which is formed according to this embodiment as a removable cap.
  • the end 112 has at its inner periphery a plurality of grooves 208 which extend along the longitudinal axis 104.
  • FIG. 2C shows the second drum 106 with the chambers 120, 122.
  • the drum 106 has on its outer wall 210 a plurality of projections 212 which extend from the outer wall 210 radially outwardly.
  • the projections 212 of the drum 106 engage in the slots 204 of the housing 102.
  • a rotation of the drum 106 is locked about the longitudinal axis 104.
  • the drum 106 is slidable along the longitudinal axis 104 in the slots 204.
  • the second drum 106 furthermore has on its outer wall 210, in particular on its end 214 facing the first drum 108, a crown-like contour 216 which comprises a multiplicity of bevels 218, 220.
  • Two bevels 218, 220 each form a point of the crown-like contour 216.
  • the ramps 218, 220 also face away from the pivot point 140 during operation of the centrifuge with the cartridge 100.
  • FIG. 2D shows a view of the second drum 106 from Figure 2C from underneath.
  • the underside 222 of the drum 106 assigned to the end 214 has a plurality of openings 224 in order to connect the chambers 120, 122 to the mixing chamber 124 of the first drum 108 in liquid, gas and / or particle form (hereinafter "conductive").
  • the openings 224 may also conductively connect the chambers 120, 122 to the chamber 126 of the first drum 108.
  • a respective conductive connection is determined by the position of a respective opening 224 with respect to the chambers 124, 126. This position is achieved by rotating the first drum 108 relative to the second drum 106, as will be explained in more detail later.
  • FIG. 2E shows a lancing device 226, which in FIG. 1 not shown.
  • Lancing device 226 includes a plate 228 having one or more spikes 230 disposed adjacent to an opening 232 in plate 228, respectively.
  • the mandrels 230 serve to control a respective opening 224 in the underside 222 of the second drum 106 in a controlled manner, whereupon in particular liquid flows from the corresponding chamber 120, 122 through the opening 232 into the chambers 124 or 126.
  • Figure 2F shows the first drum 108 with the chambers 124, 126.
  • the first drum 108 has a plurality of projections 240 on its outer wall 238.
  • the protrusions 240 are configured to engage the slots 204 (as well as the protrusions 212 of the second drum 106). As long as the projections 240 are engaged with the slots 240, rotation of the first drum 108 about the longitudinal axis 104 is disabled. However, the projections 240 along with the first drum 108 are movable along the longitudinal axis 104 in the slots 204.
  • the projections 240 have bevels 242, which point in the direction of the pivot point 140 during operation of the centrifuge with the cartridge 100 and are formed corresponding to the bevels 206 and 220.
  • FIG. 2G shows the third drum 110 with the chambers 132, 136.
  • the drum 110 has projections 244 which project from the outer wall 246 of the drum 110 respectively.
  • the projections 244 are adapted to engage the grooves 208 of the end 112 so that the drum 110 is displaceable in the longitudinal direction 104 in the grooves 208. A rotation of the drum 110 about the longitudinal axis 104 is thus locked.
  • FIG. 3A-3E show several operating conditions during operation of the cartridge 100 FIG. 1 , wherein an additional drum 302 is shown, but this is not relevant in the present case.
  • the Figures 4A-4E correspond respectively with the Figures 3A-3E and illustrate the movement of the ramps 206, 218, 220, 242 relative to each other.
  • FIG. 3B shows an operating state of the cartridge 100 which is more advanced than that in FIG FIG. 4B shown condition.
  • the housing 102 is shown partially transparent to reveal the interior.
  • the projections 200, the slots 204, the bevels 206, the projections 212, the bevels 218, 220, the projections 240 and the bevels 242 form in conjunction with the return spring 114, the above-mentioned adjusting device 300 for defined rotation of the first drum 108 relative to the other drums 106, 110 about the longitudinal axis 104.
  • FIGS. 3A and 4A show a first position in which the projections 240 of the first drum 108 engage in the slots 204 and thus a rotation of the drum 108 is locked about the longitudinal axis 104. If the rotational speed of the centrifuge is increased, then the second drum 106 pushes, by means of the bevels 220 of the contour 216, onto the bevels 242 of the first drum 108 against the action of the spring 114, compressing the spring 114. As a result, the drum 108 moves in a direction away from the pivot point 140 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
  • the spring 114 presses the first drum 108 by means of the third drum 110 again in the direction of the pivot point 140.
  • the second drum 106 together with their bevels 220 also back in Direction of the pivot point 140 moves, whereby the bevels 242 of the first drum 108 come to rest against the bevels 206 of the housing 102 and slide along this while performing a further rotational movement of the drum 108 in a third position, as in Figures 4D and 4E shown.
  • the projections 240 of the drum 108 are again located in the slots 204 of the housing 102, so that further rotation of the drum 108 about the longitudinal axis 104 is again locked.
  • the process described above may be repeated as many times as desired to rotate the first drum 108 in a defined manner relative to the other drums 106 and 110.
  • the cartridge 100 further includes an electrical switch 500.
  • This is partly in FIG. 5 shown schematically in a perspective view obliquely from above the first drum 108 from Figure 2F shows.
  • the drum 108 is without the ramps 242 and other details Fig. 2F shown.
  • the switch 500 in the FIGS. 6A to 6B which schematically shows various switching states of the switch 500 according to an embodiment of the cartridge 100 according to the invention.
  • the second drum 106 and the first drum 108 are shown.
  • the switch 500 may include first contact elements 510, 512.
  • the contact elements 510, 512 are each formed according to the embodiment as a portion of a respective associated interconnect 514.
  • the conductor tracks 514 each extend in sections along the central axis 104 on the drum wall 518, ie, for example along an inner side of the chamber 126, and in sections along the end face 516 of the drum wall 518.
  • FIG. 6A shows that the tracks 514 may also extend along an outside of the drum wall 518.
  • a respective front-side section of a conductor track 514 forms the contact elements 510 and 512, respectively.
  • the contact element 512 this may additionally include a bump 520, eg of gold, whereby the contact with a second contact element 530 is seen FIG. 6A , is further improved.
  • the conductor tracks 514 are applied to the surface of the drum wall 518 at least in sections, for example by means of vapor deposition, electroplating, plasma coating or printing. Furthermore, the interconnects 514 can be patterned by means of etching processes or laser ablation. Furthermore, the conductor tracks 514 can be glued or laminated directly or via a film. Furthermore, the conductor tracks 514 can also be cast during the manufacturing process of the first drum 108, for example by injection molding. The conductive traces 514 may also be coated with a protective layer. The conductor tracks 514 typically have a thickness of a few nanometers (eg 50 nm) up to a few millimeters (eg 3 mm) or can also be designed as wires. The width of the tracks 514 may vary from a few microns to a few millimeters.
  • the conductor tracks 514 may include metallic materials such as copper, gold, aluminum, platinum, titanium their alloys or doped semiconductor materials such as silicon.
  • the second contact element 530 is on the front side (corresponds to the bottom 222, see Fig. 2D ) of the second drum 106 and may also be formed in sections as a conductor track 514.
  • the above statements on the contact elements 510, 512 and interconnects 514 apply accordingly.
  • the first contact element 512 or the first drum 108 initially has a distance 532 with respect to the second contact element 530 and the second drum 106, respectively. This corresponds to the first position of the first drum 108, see Fig. 4A .
  • the first drum 108 enters its second position (as above in connection with Fig. 4A described).
  • the first and second drums 108, 106 and thus the first and second contact elements 512, 530 move towards each other, see Fig. 4C , At the same time, the first drum 108 rotates.
  • the contact elements 512, 530 thus reach a contact position, in which they contact each other electrically, see FIG. 6B , This closes a circuit.
  • a heater 534 which is provided adjacent to the chamber 124 in the first drum 108, thereby current flows through and thus heats a component 536 in the chamber 124.
  • the temperatures be adjusted cyclically from about 94 ° C to about 54 ° C and about 72 ° C in the component 536, for which purpose the heater 534 can be used.
  • component 536 may have been transferred from chamber 120 of second drum 106 into chamber 124 in a previous step, as described above.
  • the chambers 120, 124 may also be connected and heated simultaneously with one another for the transmission of the component 536.
  • any other electrical component could also be switched by means of the switch 500.
  • metallic components in multilayer structure and or in the form of alloys
  • CMOS complementary metal-oxide-semiconductor
  • electrodes or sensors for example ChemFETs
  • CMOS complementary metal-oxide-semiconductor
  • ChemFETs n-doped organic compound
  • the switch may be connected to a readout device 538 in the form of a microchip (semiconductor element) 538.
  • the readout device 538 registers at which times the switch 500 is closed.
  • the read-out device 538 can store the corresponding shift course.
  • the switching process can in turn be read out, for example, for quality assurance purposes from the readout device 538, in particular wirelessly.
  • the drums 106, 108 or the contact elements 512, 530 move from the contact position into the third position mentioned above, see Figures 4E and 6C , In this case, the first drum 108 is rotated a bit further, see Figure 4D , In the third position, the drums 106, 108 and the contact elements 512, 530 are again spaced from each other.
  • FIGS. 7A and 7B schematically show various switching states of a switch 500 of a cartridge 100 according to another embodiment of the invention.
  • the first contact element 510 is provided on an end face of the first drum 108, which faces away from the fulcrum 140.
  • the second contact element 530 is attached to a projection 700 which extends from the housing 102. The second contact element 530 points in the direction of the pivot point 140.
  • FIG. 7A now shows the first position of the first drum 108, see also FIG. 4A in that the contact elements 510, 530 are spaced from each other and thus open.
  • Fig. 7B shows the contact position of the contacts 510, 530, so the closed Switching state of the switch 500, see also Fig. 4C , The actuation of the first drum 108 from the first position to the contact position is speed-controlled.
  • FIGS 8A and 8B schematically show various switching states of a switch 500 of a cartridge 100 according to a still further embodiment of the invention.
  • the first drum 108 includes a nose 800 that extends radially outward from the drum wall 518 with respect to the central axis 104.
  • the first contact element 510 is arranged on the nose 800 and points in the direction of the pivot point 140.
  • the second contact element 530 is provided on a nose 700 of the housing 102 and points in a direction away from the pivot point 140.
  • the contact elements 510, 530 are brought into contact with each other, wherein the first drum 108 is moved along the longitudinal axis 104 in a direction away from the pivot point 140 and rotated thereafter, see Figures 8B . 4B and 4C , whereby the contact element 510 passes under the contact element 530.
  • the drum 108 again moves in the direction of the pivot point 140, see FIG. 4C , As a result, the contacts 510, 530 come into contact with each other, see FIG.
  • the nose 800 engages behind the nose 700, so that - due to the action of the spring 114 - even at a reduced speed, the contact elements 510, 530 remain in contact. Only when the speed is increased again, the contact element 510 is lifted from the contact element 530 again and the nose 800 continues to rotate, whereby the lugs 700, 800 disengaged.
  • FIG. 9 illustrates that instead of or in addition to the centrifugal force 142, an actuator 900 can be provided, which ensures the rotational movement of the first drum 108.
  • the actuator 900, the projections 200, the slots 204, the slopes 206, the projections 212, the bevels 218, 220, the projections 240 and the bevels 242 form in conjunction with the return spring 114 in this embodiment, the above-mentioned adjustment 300 to defined Rotating the first drum 108 relative to the other drums 106, 110 about the longitudinal axis 104.
  • the drum 106 pushes by means of the bevels 220 of the contour 216, s. Fig. 4A , on the slopes 242 of the drum 108 against the action of the spring 114, wherein the spring 114 is compressed.
  • the drum 108 moves in a direction away from the pivot point 140 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
  • rotation of the drum 108 about the longitudinal axis 104 is enabled, as in FIG FIG. 4C illustrated.
  • a force component results which automatically rotates the drum 108 as it enters the second position, as indicated at FIG. 4C indicated by arrows pointing to the left.
  • the spring 114 again presses the first drum 108 in the direction of the pivot point 140 by means of the third drum 110.
  • the second drum 106 together with its bevels 220 is also moved again in the direction of the pivot point 140 whereby the bevels 242 of the first drum 108 come to lie against the bevels 206 of the housing 102 and slide along this, while making a further rotational movement of the first drum 108 in the third position, as in Figures 4D and 4E shown.
  • the actuator 900 may be electrically, mechanically and / or pressure-operated.
  • a piezoelectric, electrostatic, semi-mechanical-manually or electromagnetically operated actuator 900 is suitable.
  • “Operated” here means the active principle, which utilizes the actuator 900 to generate the actuating force for actuating the drum 106 (or, depending on the embodiment, also one of the drums 108 or 110).
  • the actuator 900 may include an electromagnet that cooperates with a metal part disposed in one of the drums 106, 108, 110 that the electromagnet attracts or repels by appropriately driving it, thereby effecting the above-described adjustment of the drums 106, 108, 110 to reach each other.
  • the compressive force applied to the second drum 106 by means of the actuator 900 is typically 0.5-100 N. The compressive force to be applied by the actuator is reduced according to the centrifugal force acting.
  • a suitable, not shown, control device which controls the actuator 900, so that the drums 106, 108, 110 occupy the desired position to each other at the desired time.
  • the control device may have a timer and / or an integrated circuit.
  • first drum 108 and the second drum 106 are provided.
  • An actuator in the form of a shaft is connected on the one hand to the actuator 900 and on the other hand to the first drum 108.
  • the actuator 900 in particular an electric motor, thereby rotates the shaft and thereby the first drum 108 about the central axis 104, whereby different chambers 120, 122, 124 are conductively connected to each other, as described above.
  • a ballpoint pen mechanism is not provided in this embodiment.
  • the actuator 900 may be further configured to also move the shaft along the centerline 104 to thereby complain of the first drum 108 from the second drum 106 for rotation and to press the drums 106, 108 together again after rotation, thereby a sealed, conductive connection is provided, for example, between the chamber 120 and the chamber 124 and / or the switch 500 is closed.
  • FIG. 10 shows in a schematic section a centrifuge 1000 according to an embodiment of the present invention.
  • the centrifuge 1000 has coils 1002, which can be integrated, for example, in a lid and / or bottom of the centrifuge. By means of the coils 1002, the current, for example for operating the heater 534, is coupled into the cartridge 100.
  • the cartridge 100 has one or more coils, not shown.
  • the cartridge 100 is inserted into a rotor 1004 of the centrifuge 1000.
  • the power supply can be designed as a reusable component or device, which is part of this during operation of the cartridge 100.
  • the lid 118 can be detachably provided with an integrated accumulator.
  • a sensor in the housing 102 of the cartridge 100 can be designed so that it can be removed from the housing 102 after the biochemical processes have been carried out and the sensor data can then be read out externally.
  • measurement signals of the sensors or cartridge 100 can be forwarded to the outside of the cartridge 100 via a transmission device (for example by means of an RFID chip). This allows the implementation of real-time measurements (real-time).
  • the centrifuge 1000 can detect which cartridge type has been loaded by means of an RFID chip in the cartridge 100 and thus automatically use the correct processing protocol (e.g., frequency log with acceleration and deceleration ramps, target frequencies, and hold times).
  • the second drum 106 and / or the third drum 110 may be fixed or movable with respect to the housing 102.
  • the drums 106, 110 may be provided, for example, each rotatable about the central axis 104 by means of a further actuator.
  • the traces 514 may be e.g. run in the housing 102 and are contacted directly with sensors which are provided in non-rotatable drums (for example, the second drum 106).
  • non-rotatable drums for example, the second drum 106
  • the switch 500 may in principle be formed in particular between any two drums 106, 108, 110 or any drum 106, 108, 110 and the housing 102.
  • the mixing chamber 124 may include an obstruction structure, not shown, such as a sieve or grid structure configured to move through the component 536 under the influence of a centrifugal force (ie, when the rotational speed of the centrifuge exceeds a predetermined threshold) to mix through.
  • an obstruction structure such as a sieve or grid structure configured to move through the component 536 under the influence of a centrifugal force (ie, when the rotational speed of the centrifuge exceeds a predetermined threshold) to mix through.
  • the housing 102 and the drums 106, 108, 110 may be made of the same or different polymers.
  • the one or more polymers are, in particular, thermoplastics, elastomers or thermoplastic elastomers. Examples are cycloolefin polymer (COP), cycloolefin copolymer (COC), polycarbonates (PC), polyamides (PA), polyurethanes (PU), polypropylene (PP), polyethylene terephthalate (PET) or poly (methyl methacrylate) ( PMMA).
  • COP cycloolefin polymer
  • COC cycloolefin copolymer
  • PC polycarbonates
  • PA polyamides
  • PU polyurethanes
  • PP polypropylene
  • PET polyethylene terephthalate
  • PMMA poly (methyl methacrylate)
  • the second drum 106 and / or the third drum 110 may be formed integrally with the housing 102.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Software Systems (AREA)
  • Centrifugal Separators (AREA)

Claims (15)

  1. Cartouche (100) présentant
    un premier tambour (108) doté d'une première chambre (124),
    un dispositif d'ajustement (300) conçu pour faire tourner le premier tambour (108) autour de son axe central (104) pour ainsi relier la première chambre (124) à une deuxième chambre (120, 122) de manière à pouvoir conduire des liquides, des gaz et/ou des particules, et
    un commutateur électrique (500) qui peut être actionné entre une position de commutation fermée et une position de commutation ouverte au moyen du dispositif d'ajustement (300).
  2. Cartouche selon la revendication 1, dans laquelle le dispositif d'ajustement (300) présente une première pente (220) qui coopère avec une deuxième pente (242) du premier tambour (108) pour amener le premier tambour (108) depuis une première position dans laquelle la deuxième pente (242) engage en correspondance géométrique un boîtier (102) de la cartouche (100) dans le sens de rotation autour de l'axe central (104) et, en opposition à l'effet d'un moyen de rappel (114), l'amener dans une deuxième position située sur l'axe central (104) et dans laquelle la correspondance géométrique est supprimée et le premier tambour (108) tourne autour de l'axe central (104).
  3. Cartouche selon l'une des revendications précédentes, dans laquelle la deuxième chambre (120, 122) et/ou une troisième chambre (132, 136) sont montées en amont ou en aval du premier tambour (108) sur l'axe central (104), de préférence la première chambre (124) pouvant être raccordée sélectivement à la deuxième chambre (120, 122) ou à la troisième chambre (132, 136) au moyen du dispositif d'ajustement (300) de manière à conduire des liquides, des gaz et/ou des particules.
  4. Cartouche selon l'une des revendications précédentes, dans laquelle un deuxième tambour (106) doté de la deuxième chambre (120, 122) et/ou un troisième tambour (110) doté de la troisième chambre (132, 136) sont prévus, de préférence le deuxième tambour (106) étant monté en amont du premier tambour (108) sur l'axe central (104) et/ou le troisième tambour (110) étant monté en aval du premier tambour (108).
  5. Cartouche selon l'une des revendications précédentes, dans laquelle le commutateur (500) comporte au moins un premier et un deuxième élément de contact (510, 512, 530) qui sont mis en contact mutuel lorsque le commutateur (500) est en position fermée et sont maintenus à distance mutuelle lorsque le commutateur (500) est en position ouverte, le premier élément de contact (510, 512) étant placé sur le côté frontal (516) du premier tambour (108) et le deuxième élément de contact (530) sur un côté frontal (222) du deuxième ou du troisième tambour (106, 110) tourné vers le côté frontal (516) du premier tambour (108) ou le premier élément de contact (510) est placé sur le premier, le deuxième ou le troisième tambour (106, 108, 110) et le deuxième élément de contact (530) sur un boîtier (102) de la cartouche (100) et en particulier sur une saillie (700) de ce boîtier.
  6. Cartouche selon la revendication 5, dans laquelle le commutateur (500) comporte en plus du premier élément de contact (510) au moins un autre élément de contact (512) qui sont mis sélectivement en contact avec le deuxième élément de contact (530) par rotation du premier tambour (108).
  7. Cartouche selon l'une des revendications précédentes, dans laquelle le premier et le deuxième élément de contact (510, 512, 530) sont mis en contact mutuel dans une position de contact du premier tambour (108) qui se raccorde à la deuxième position du premier tambour (108), de préférence le premier tambour (108) et le boîtier (102), en particulier des saillies (700, 800) de ce dernier, s'engageant les uns derrière les autres dans la position de contact pour éviter un déplacement autonome du premier tambour (108) depuis sa position de contact jusque dans une troisième position suite à l'effet du moyen de rappel (114).
  8. Cartouche selon l'une des revendications précédentes, dans laquelle le premier et le deuxième élément de contact (510, 512, 530) comportent au moins certains tronçons d'une piste conductrice (514) et/ou de plus au moins une bosse métallique (520).
  9. Cartouche selon l'une des revendications précédentes, dans laquelle le commutateur (500) peut être raccordé à un dispositif de lecture (538) conçu pour lire la situation de commutation du commutateur (500) et/ou conserver de plus en mémoire l'évolution des commutations du commutateur (500).
  10. Cartouche selon l'une des revendications précédentes, présentant en outre un dispositif de chauffage (534) prévu en particulier dans ou sur un boîtier (102) de la cartouche (100) du premier, du deuxième et/ou du troisième tambour (108, 106, 110), en particulier pour le chauffage cyclique de la première, de la deuxième et/ou de la troisième chambre (120, 122, 124, 126, 132, 136), et apte à être commuté en chauffage par actionnement du commutateur (500).
  11. Cartouche selon l'une des revendications précédentes, présentant en outre un composant semiconducteur prévu en particulier dans ou sur un boîtier (102) de la cartouche (100) du premier, du deuxième et/ou du troisième tambour (108, 106, 110) et apte à être commandé par actionnement du commutateur (500).
  12. Cartouche selon l'une des revendications précédentes, dans laquelle la cartouche (100) est configurée pour être insérée dans une centrifugeuse (1000) et pour être centrifugée, le dispositif d'ajustement (300) étant conçu pour actionner le premier tambour (108) en vue de sa rotation autour de l'axe central (104) lorsque la force centrifuge (142) n'atteint pas une valeur de seuil prédéterminée, ou le dispositif d'ajustement (300) présente un actionneur (900) qui actionne le premier tambour (108) en vue de la rotation autour de l'axe central (104).
  13. Cartouche selon l'une des revendications précédentes, dans laquelle le commutateur (500) peut être relié à une source d'énergie par fil ou sans fil pour entraîner l'écoulement d'un courant dans le commutateur (500) lorsque ce dernier est en position fermée.
  14. Centrifugeuse (1000) présentant une cartouche (100) selon l'une des revendications précédentes.
  15. Procédé de traitement d'au moins un composant (536) placé dans une cartouche (100) selon l'une des revendications 1 à 13, le procédé présentant les étapes suivantes :
    prévoir un premier tambour (108) qui présente une première chambre (124),
    prévoir un dispositif d'ajustement (300) conçu pour faire tourner le premier tambour (108) autour de son axe central (104) et ainsi relier la première chambre (124) à une deuxième chambre (120, 122) de manière à pouvoir conduire des liquides, des gaz et/ou des particules et
    actionner un commutateur électrique (500) entre une position de commutation fermée et une position de commutation ouverte au moyen du dispositif d'ajustement (300).
EP12163525.4A 2011-06-07 2012-04-10 Cartouche, centrifugeuse et procédé Not-in-force EP2532427B1 (fr)

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EP2532427A2 EP2532427A2 (fr) 2012-12-12
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DE102010003223B4 (de) * 2010-03-24 2014-09-18 Albert-Ludwigs-Universität Freiburg Vorrichtung zum Einsetzen in einen Rotor einer Zentrifuge, Zentrifuge und Verfahren zum fluidischen Koppeln von Kavitäten
DE102011077134A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren zum Mischen einer ersten und zweiten Komponente
DE102011077124A1 (de) * 2011-06-07 2012-12-13 Robert Bosch Gmbh Kartusche, Zentrifuge sowie Verfahren
DE102012221734A1 (de) * 2012-11-28 2014-05-28 Robert Bosch Gmbh Kartusche mit elektrischem Schleifkontakt sowie Verfahren
DE102013220064B3 (de) * 2013-10-02 2014-12-24 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Vorrichtung und verfahren zum bewegen einer festphase in eine mehrzahl von kammern
RU2639906C1 (ru) * 2017-05-05 2017-12-25 Катарина Валерьевна Найгерт Смеситель-дозатор с магнитожидкостными управляющими элементами
DE202018101760U1 (de) 2018-03-28 2019-07-01 Sigma Laborzentrifugen Gmbh Laborzentrifuge und Zentrifugenbehälter für eine Laborzentrifuge
RU2767588C1 (ru) * 2021-03-15 2022-03-17 Федеральное государственное бюджетное учреждение науки Институт проблем управления им. В.А. Трапезникова Российской академии наук Устройство смешения и дозирования жидких компонентов в заданном соотношении
CN116474856B (zh) * 2023-05-11 2024-03-08 首都医科大学 一种具有振荡功能的试管架

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Publication number Publication date
US20120314532A1 (en) 2012-12-13
CN102814241B (zh) 2016-05-04
EP2532427A2 (fr) 2012-12-12
DE102011077115A1 (de) 2012-12-13
EP2532427A3 (fr) 2013-03-27
US9399214B2 (en) 2016-07-26
CN102814241A (zh) 2012-12-12

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