WO2014117908A1 - Dispositif d'extraction de liquides organiques préstockés à sec dans un prélèvement, cartouche et procédé - Google Patents

Dispositif d'extraction de liquides organiques préstockés à sec dans un prélèvement, cartouche et procédé Download PDF

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Publication number
WO2014117908A1
WO2014117908A1 PCT/EP2013/078086 EP2013078086W WO2014117908A1 WO 2014117908 A1 WO2014117908 A1 WO 2014117908A1 EP 2013078086 W EP2013078086 W EP 2013078086W WO 2014117908 A1 WO2014117908 A1 WO 2014117908A1
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WO
WIPO (PCT)
Prior art keywords
sample
body fluid
extraction
dry
upstream
Prior art date
Application number
PCT/EP2013/078086
Other languages
German (de)
English (en)
Inventor
Melanie HOEHL
Martina Daub
Juergen Steigert
Original Assignee
Robert Bosch Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch Gmbh filed Critical Robert Bosch Gmbh
Priority to EP13814576.8A priority Critical patent/EP2951277B1/fr
Publication of WO2014117908A1 publication Critical patent/WO2014117908A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves

Definitions

  • Liquids Liquids. Typically, this manipulation is done manually with tools such as pipettes, reaction vessels, active probe surfaces or laboratory equipment. By tilting robots or special equipment, these processes are already partially automated.
  • Lab-on-a-Chip systems are microfluidic systems that accommodate the entire functionality of a macroscopic laboratory on a plastic card-sized plastic substrate.
  • Lab-on-a-chip systems typically consist of two
  • a test carrier or disposable cartridge includes structures and mechanisms for the implementation of basic fluidic operations (e.g., mixers), which may consist of passive components such as channels, reaction chamber, upstream reagents, or even active components such as valves or pumps.
  • the second main component is actuation, detection and control units.
  • One of the primary means used to diagnose a person's condition is the analysis of the person's blood or other bodily fluids for the presence or absence of "analytes" indicative of the person's health.
  • Certain body fluids, such as urine, saliva, semen, and the like a. can be obtained noninvasively, and the analysis of such fluids can
  • Body fluid which, however, usually provides the most complete picture of a person's health, is the whole blood.
  • the process is cumbersome and expensive because it requires the blood or other sample to be collected either in a location that has all the equipment required, or that the sample is cooled and shipped to a separate laboratory for further processing. It also takes a considerable amount of time to obtain the analysis results. However, time can be critical to the life or health of the patient.
  • DBS Dried Blood Spots
  • DBS digital filter spherical blood
  • a few drops of capillary blood are applied to simple filter paper.
  • the use of DBS has several advantages over the classical whole blood / plasma sample, which is currently still the most common type of sample.
  • One of the greatest advantages is the possibility of storing the DBS at room temperature, which simplifies the transport from the patient to the analytical laboratory and makes it more cost-effective.
  • Another advantage is the small sample volume required of 10 to 30 ⁇ blood for DBS compared to 100 to 500 ⁇ for the plasma samples. This advantage is particularly important when children or small animals, such as rodents, should be sampled.
  • Dry blood in a form acceptable to the diagnosis (usually liquid) from filter material can be expensive. It may also be difficult to separate the dissolved aqueous sample from the solid filter material. Once the analyte is dissolved, sometimes another purification step is needed. All of these steps require many disposable parts (e.g., pipette tips, MTP, plastic containers) and process steps that often involve multiple different devices.
  • Cartridge and the method defined in claim 1 1 have the advantage over conventional solutions that it is now possible to dry To extract upstream samples (such as DBS samples) for any dry bodily fluids in a microfluidic system by means of a LoC system in a simple manner and optionally further below
  • Body fluids in a sample smaller sample volumes sufficient for an analysis, so that the method is especially suitable for infants or small animals or even with repeated removal of samples for the patient is gentler. Therefore, with the present device, advantageously dry bodily fluids in a sample can be examined, which are obtained in a less invasive and patient-friendly manner.
  • the present device is particularly advantageous for the extraction of dry bodily fluids of all kinds from a sample such as urine, sputum, lavage, saliva, ejaculate / sperm, pericardial fluid, pleural fluid, abdominal fluid, peritoneal dialysate (CAPD), drainage fluid,
  • a sample such as urine, sputum, lavage, saliva, ejaculate / sperm, pericardial fluid, pleural fluid, abdominal fluid, peritoneal dialysate (CAPD), drainage fluid,
  • Bronchoalveolar lavage BALF
  • cerebrospinal fluid gastric juice
  • sweat lymph
  • bile pancreatic secretions
  • blood breast milk
  • vaginal secretions of the woman tears, nasal secretions, brain water, ascites, pleural fluid, amniotic fluid, earwax, pus, synovium, menstrual fluid, aqueous humor of the eye, etc ,
  • Possible applications for the device according to the invention for the extraction of dry body fluid in a sample include, for example:
  • Infection diagnostics and therapy support and screening for example HIV, HPV, STD, hepatitis etc.
  • the extraction of the dry body fluid upstream of the sample by incubation in an elution solution realizes soaking of the carrier material together with the dry body fluid of the sample until the analytes have dissolved from the carrier material. It can do that
  • Soaking can also be done at elevated temperatures.
  • various water-based or organic solutions come into question.
  • alcohols especially ethanol, butonal, isopropanol, methanol, etc
  • Buffer solutions with water for example PBS, Tween, HEPES and sodium azide
  • organic substances for example organic acids or bases etc.
  • protein-containing solutions for example milk, BSA, calcein
  • inorganic solutions for example acids and bases
  • the extraction of the dry body fluid upstream of the sample by means of a pumping function of an elution solution through the sample over a predetermined time takes place.
  • the extraction can be effected by means of a pumping function by the elution solution for the predetermined time by the sample, in particular the carrier material of the sample, reciprocated back and forth.
  • the extraction of the dry body fluid upstream of the sample takes place by means of ultrasound.
  • ultrasound should be used with a frequency which both leads to the extraction of body fluid from the carrier material and excludes damage or alteration of the body fluid during the extraction.
  • frequencies in a range of 20 kHz to 50 MHz should be used.
  • the power density should preferably be less than 1 W / cm 2 .
  • the extraction of the dry body fluid upstream of the sample by means of enzymatic digestion of the carrier material by enzymes.
  • the extraction of the dry-upstream body fluid from the carrier material can be accomplished by a specific enzymatic digestion of the carrier material by enzymes.
  • enzymes may be proteases, proteinases or
  • proteolytic enzymes such as those used for the cleavage of, for example, proteins or peptides, in biological or bio-chemical processes.
  • the sample is placed horizontally, vertically or inclined relative to the device on the receiving area.
  • the device according to the invention may require a differently oriented recording of the sample on the device.
  • the device may require a differently oriented recording of the sample on the device.
  • the receiving area to be pivotally formed relative to the Lab On Chip system has a carrier which is pivotally coupled to the Lab On Chip system and is used for receiving the sample.
  • the device according to the invention is the
  • a filter or a purification device for the body fluid extracted from the sample is provided.
  • the purification device for example in the delivery area of the lab-on-chip system, in operative connection with a microfluidic channel, which connects the receiving area to the delivery area, a DNA purification, a protein purification, an ion exchanger, an antibody-based purification of blood components, etc . be provided.
  • the carrier material of the sample is made of a degradable or dissolvable material, of a filter material or consists of a polymer.
  • a degradable material preferably an enzymatic-digestible material is provided, such as cellulose.
  • a dissolvable material preferably a liquid-soluble material is provided, such as gelatin.
  • filter materials it is possible to use surface filters or fabric filters which comprise, for example, PVDF, silica matrix, felts, spunbonded nonwovens, paper, woven fabrics, synthetic fibers
  • the carrier material of the sample consists of glass as fibers or of glass beads.
  • Glass beads may each be provided with a smooth, rough or textured surface, including blends of differently shaped ones
  • the different carrier materials can also be processed chemically and / or physically so as to be able to provide a desired property.
  • a cartridge comprising a device according to the invention for the extraction of dry body fluid upstream in a sample, a housing of the cartridge, a drum having at least one chamber, wherein the device is disposed inside the drum.
  • Preventive body fluids in a sample is no longer necessary, since all process steps now take place in the LoC system, which is much cheaper to purchase. Furthermore, further steps for the preparation of the extracted body fluid from the sample can be realized in the cartridge, so that the processed body fluid can be provided for a subsequent analysis.
  • a method for extracting a dry body fluid from a sample comprising the steps of:
  • the extraction of the dry body fluid upstream of the sample is carried out by incubation in a Elutionslosung. This extraction process realizes soaking of the carrier material together with the dry body fluid of the sample until the analytes have dissolved from the carrier material. It can do that
  • Soaking can also be done at elevated temperatures.
  • the above-described water-based or organic solutions come into question.
  • the extraction of the dry body fluid upstream of the sample by means of a pumping function of a Elutionslosung carried out by the sample over a predetermined time can be carried out by means of a pumping function by the elution solution for the predetermined time by the sample, in particular the support material of the sample, pumped back and forth.
  • Extraction process is possible with control of temperature and / or pressure during extraction.
  • the extraction of the dry body fluid upstream of the sample takes place by means of ultrasound.
  • ultrasound should be used with a frequency which both leads to the extraction of body fluid from the carrier material and excludes damage or alteration of the body fluid during the extraction.
  • the extraction of the dry body fluid upstream of the carrier material can be carried out by a specific, enzymatic digestion of the carrier material by enzymes.
  • FIG. 1 shows a schematic perspective view of a device according to the invention for the extraction of dry body fluid in a sample
  • FIG. 2 shows a schematic perspective view of the device according to FIG. 1, wherein the sample is placed in a receiving area of the device, FIG.
  • Figs. 3A to 3C is a schematic view of extraction methods for extracting dry body fluid from a sample according to the present invention
  • FIG. 4 shows a schematic view of a further extraction process for a dry body fluid in a sample according to the present invention
  • FIG. 5 shows a schematic view of a region of the device according to the invention, which is used for the purification of an extracted body fluid from the sample
  • FIG. 6 shows a cartridge with a device according to the invention for the extraction of dry body fluid in a sample according to the present invention.
  • FIG. 1 shows, in a simplified and schematic illustration, a device 100 according to the invention for extracting dry body fluid (not shown) in a sample 30.
  • the device 100 has a lab-on-chip system 10 with a receiving region 20, which holds the sample 30 with the
  • Body fluid in dry pre-stored form in a carrier material is provided. Furthermore, the lab-on-chip system 10 has a delivery region 50 for delivering the extracted body fluid from the sample 30, which is operatively connected to the acquisition region 20 via a microfluidic channel (not shown). The extraction of the dry body fluid from the sample 30 takes place in the receiving area 20.
  • the use of a DBS pellet for sample 30 is described below by way of example. However, for use in the present apparatus 100, any types of samples containing dry body fluid may be used.
  • the pellet 30 is inserted into the LoC system 10, namely into the receiving area 20.
  • the pellet 30 can be inserted into the receiving area 20 either horizontally, obliquely or vertically.
  • FIG. 2 shows a schematic perspective view of the device 100 according to FIG. 1, wherein the pellet 30 is placed in the receiving region 20 of the device 100. After placing the pellet 30 in the receiving area 20, the blood sample is extracted. The extraction can take place in different ways. Subsequently, the extracted blood sample is delivered to the delivery area 50 of the device 100 to the environment (not shown), preferably by means of a hose (not shown).
  • Figs. 3A to 3C show a schematic view of extraction methods for extraction of dry body fluid from a sample according to the present invention.
  • FIG. 3A shows a first method according to the invention for extracting a dry body fluid (not shown) from a sample 30
  • Sample 30 is separated from the carrier material 40 of the sample 30 by incubation in an elution solution.
  • This extraction process realizes soaking of the carrier material 40 together with the dry body fluid of the sample 30 until the analytes have dissolved from the carrier material 40.
  • the soaking can be done even at elevated temperatures.
  • various water-based or organic solutions come into question.
  • alcohols in particular ethanol, butanol, isopropanol, methanol, etc.
  • buffer solutions with water for example PBS, Tween, HEPES and sodium azide
  • organic substances for example organic acids or bases etc.
  • protein-containing solutions for Example, milk, BSA, calcein
  • inorganic solutions for example acids and bases
  • Fig. 3B shows a second method according to the invention for extracting a dry body fluid (not shown) by means of a pumping function.
  • an elution solution 60 is pumped back and forth for a predetermined time by a sample 30, in particular the carrier material 40 of the sample 30.
  • An optimization of Efficiency of the extraction method of the invention is possible with control of temperature and / or pressure during extraction.
  • 3C shows a third method according to the invention for extracting a dry body fluid (not shown) from a sample 30 by means of ultrasound.
  • ultrasound should be used with a frequency which both leads to the extraction of body fluid from the carrier material 40 of the sample 30 and excludes damage or change of body fluid during the extraction. It is preferable to use frequencies in a range of 20 kHz to 50 MHz.
  • the power density should preferably be less than 1 W / cm 2 .
  • FIG. 4 shows a schematic view of a fourth extraction process for a dry body fluid (not shown) in a sample 30 according to the present invention.
  • a blood sample in the form of a pellet 30 is stored on an enzymatically digestible cellulosic carrier material 40.
  • the extraction of the blood sample from the carrier material 40 takes place by specific, enzymatic digestion of the carrier material 40 by enzymes 70.
  • enzymes 70 As an example of such enzymes proteases, proteinases or proteolytic enzymes may be mentioned, as they are used for the cleavage of, for example, proteins or peptides, in biological or bio-chemical processes.
  • Fig. 5 shows a schematic view of a portion of the invention
  • a filter function 80 is arranged downstream in a microfluidic channel 90.
  • a purification function (not shown), such as DNA purification, protein purification, ion exchange, antibody-based purification of blood components, etc., may also be provided in the microfluidic channel 90.
  • FIG. 6 shows a cartridge 200 with a device according to the invention (not shown) for extracting dry body fluid (not shown) in a sample 30.
  • the cartridge 200 comprises a housing 210, which has a housing
  • the cartridge 200 has in its interior three drums 220, 221, 222, which along the axis of rotation 235 of the cartridge 200 on top of each other and are arranged congruent to each other.
  • Each drum 220, 221, 222 in turn has at least two chambers 230, 231 in its interior, which are provided for receiving different reagents and for one or more analytes (here the DBS pellet 30).
  • the individual drums 220, 221, 222 are rotated along the rotational axis 235 of the cartridge 200 by the action of centrifugal force by means of a so-called "ballpoint pen mechanism" (not shown).
  • FIG. 6 shows a possible embodiment for a drum 220 in which the DBS pellet 30 or other dry-stored body fluid is introduced into the drum 220 from above.
  • the right side of FIG. 6 shows another embodiment of a drum 220 in which the DBS pellet 30 is inserted laterally through an opening 240 in the drum 220.
  • the proposed device according to the invention for the extraction of dry bodily fluid in the LoC system can be located in a chamber 230 of one of the drums 220, 221, 222.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un dispositif (100) d'extraction de liquides organiques préstockés à sec dans un prélèvement, ledit dispositif présentant un système de laboratoire sur puce (10) pourvu d'une zone de réception (20) destinée à la réception d'un prélèvement (30) doté d'au moins un liquide organique sous une forme préstocké à sec dans un matériau de support (40), et pourvu d'une zone de délivrance (50) destinée à la délivrance du liquide organique extrait du prélèvement (30), du liquide organique préstocké à sec étant extrait du prélèvement (30) dans la zone de réception (20).
PCT/EP2013/078086 2013-01-30 2013-12-27 Dispositif d'extraction de liquides organiques préstockés à sec dans un prélèvement, cartouche et procédé WO2014117908A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP13814576.8A EP2951277B1 (fr) 2013-01-30 2013-12-27 Procédé d'extraction de liquides organiques préstockés à sec dans un prélèvement

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102013201505.4A DE102013201505A1 (de) 2013-01-30 2013-01-30 Vorrichtung zur Extraktion von trocken vorgelagerter Körperflüssigkeit in einer Probe, Kartusche sowie Verfahren
DE102013201505.4 2013-01-30

Publications (1)

Publication Number Publication Date
WO2014117908A1 true WO2014117908A1 (fr) 2014-08-07

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PCT/EP2013/078086 WO2014117908A1 (fr) 2013-01-30 2013-12-27 Dispositif d'extraction de liquides organiques préstockés à sec dans un prélèvement, cartouche et procédé

Country Status (3)

Country Link
EP (1) EP2951277B1 (fr)
DE (1) DE102013201505A1 (fr)
WO (1) WO2014117908A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111565846A (zh) * 2018-01-15 2020-08-21 罗伯特·博世有限公司 在微流体装置中提供物质的溶液的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3478412T3 (da) * 2016-06-30 2020-07-13 Sarstedt Ag & Co Kg Anordning til tilvejebringelse af absorberende prøveholdere med en mængde tørret væske, særligt blod
CN108393105B (zh) * 2018-04-20 2023-08-25 华南师范大学 一种微流控芯片及其控制系统、控制方法
DE102022206246A1 (de) 2022-06-22 2023-12-28 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidiksystem mit Ionenaustauscher-Mischbettharz

Citations (4)

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Publication number Priority date Publication date Assignee Title
DE102008042581A1 (de) * 2008-10-02 2010-04-08 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Mikrofluidische Extraktions- und Reaktionsvorrichtung
WO2011137533A1 (fr) * 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Procédé de traitement d'échantillons séchés utilisant un dispositif microfluidique numérique
WO2012149314A1 (fr) * 2011-04-28 2012-11-01 Labcyte Inc. Récipients à échantillon appropriés pour les éjections acoustiques et la préservation des échantillons, et procédés de préservation des échantillons.
EP2532428A2 (fr) * 2011-06-07 2012-12-12 Robert Bosch Gmbh Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008042581A1 (de) * 2008-10-02 2010-04-08 INSTITUT FüR MIKROTECHNIK MAINZ GMBH Mikrofluidische Extraktions- und Reaktionsvorrichtung
WO2011137533A1 (fr) * 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Procédé de traitement d'échantillons séchés utilisant un dispositif microfluidique numérique
WO2012149314A1 (fr) * 2011-04-28 2012-11-01 Labcyte Inc. Récipients à échantillon appropriés pour les éjections acoustiques et la préservation des échantillons, et procédés de préservation des échantillons.
EP2532428A2 (fr) * 2011-06-07 2012-12-12 Robert Bosch Gmbh Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111565846A (zh) * 2018-01-15 2020-08-21 罗伯特·博世有限公司 在微流体装置中提供物质的溶液的方法
CN111565846B (zh) * 2018-01-15 2021-10-01 罗伯特·博世有限公司 在微流体装置中提供物质的溶液的方法
US11389794B2 (en) 2018-01-15 2022-07-19 Robert Bosch Gmbh Method for providing a solution of the substance in a microfluidic device

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Publication number Publication date
DE102013201505A1 (de) 2014-07-31
EP2951277B1 (fr) 2021-04-14
EP2951277A1 (fr) 2015-12-09

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