EP2532428A2 - Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant - Google Patents

Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant Download PDF

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Publication number
EP2532428A2
EP2532428A2 EP12163532A EP12163532A EP2532428A2 EP 2532428 A2 EP2532428 A2 EP 2532428A2 EP 12163532 A EP12163532 A EP 12163532A EP 12163532 A EP12163532 A EP 12163532A EP 2532428 A2 EP2532428 A2 EP 2532428A2
Authority
EP
European Patent Office
Prior art keywords
cartridge
drum
chamber
particles
centrifuge
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP12163532A
Other languages
German (de)
English (en)
Other versions
EP2532428B1 (fr
EP2532428A3 (fr
Inventor
Martina Daub
Juergen Steigert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2532428A2 publication Critical patent/EP2532428A2/fr
Publication of EP2532428A3 publication Critical patent/EP2532428A3/fr
Application granted granted Critical
Publication of EP2532428B1 publication Critical patent/EP2532428B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/15—Use of centrifuges for mixing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
    • B01F29/32—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor
    • B01F29/321—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor of test-tubes or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/42—Mixers with shaking, oscillating, or vibrating mechanisms with pendulum stirrers, i.e. with stirrers suspended so as to oscillate about fixed points or axes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/453—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements
    • B01F33/4533—Magnetic mixers; Mixers with magnetically driven stirrers using supported or suspended stirring elements supporting the stirring element in one point
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/713—Feed mechanisms comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges
    • B01F35/7137—Piercing, perforating or melting membranes or closures which seal the compartments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/716—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components
    • B01F35/7161—Feed mechanisms characterised by the relative arrangement of the containers for feeding or mixing the components the containers being connected coaxially before contacting the contents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/71—Feed mechanisms
    • B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
    • B01F35/71725—Feed mechanisms characterised by the means for feeding the components to the mixer using centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0841—Drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Definitions

  • the document describes WO 2007066783 a microchip comprising a mixing chamber.
  • the mixing chamber contains particles which are moved under the action of a centrifugal force.
  • the cartridge defined in claim 1, the centrifuge defined in claim 10 and the method defined in claim 14 have the advantage over conventional solutions that the cartridge with the first and second components is inserted into the centrifuge and thereafter the components under the action the electromagnetic force can be easily mixed. This can be done at a constant or varying speed, that is, mixing can occur regardless of the speed and the centrifugal force acting thereon.
  • Component in this case means a liquid, a gas or a particle.
  • first and second component only two different states of the same substance may be meant:
  • the first component may be formed as a clumped portion and the second component as a liquid portion of the same substance.
  • Electromagnetic force in this case means the force acting on an electrically charged material in an electric field or a magnet, in particular permanent magnets, or a current-carrying conductor, in particular coil, in a magnetic field.
  • the "means for generating the electromagnetic force" may be formed as a permanent magnet, coil or capacitance.
  • the corresponding magnetic fields are a field strength of typically 10 - 300 mT.
  • electromagnetic particles are understood as meaning particles which comprise an electrically charged material or a magnetic or a magnetizable material, for example iron or nickel.
  • the cartridge further comprises: a first drum having a first chamber, an adjusting device, which is adapted to rotate the first drum about the central axis thereof when the centrifugal force exceeds a predetermined threshold, thereby the first chamber with a second chamber to conductively connect, wherein the first and / or second chamber is formed as the mixing chamber.
  • the first and / or second component can thus be transferred between the first and second chambers, with appropriate selection of the rotational speed of a rotor of a centrifuge with the cartridge.
  • the corresponding components in the first and / or second chamber can be effectively mixed.
  • conductive is meant liquid, gas and / or particle-conducting in the present case.
  • the adjusting device comprises a first bevel, which cooperates with a second bevel of the first drum to this from a first position in which this is in a form-fitting manner with a housing of the cartridge in the rotational direction about the central axis, to spend in a second position along the central axis and against the action of a return means in which the positive connection is canceled and the first drum rotates about the central axis.
  • a simple mechanism is provided for adjusting the first drum about at least two defined positions in the rotational direction about the central axis.
  • the mixing chamber can thus be the first drum forward and / or downstream or also provided in the first drum itself.
  • the mixing chamber can preferably be optionally connected to different further chambers as required.
  • a second drum which has the second chamber
  • a third drum which has the third chamber.
  • the second drum may also have the second chamber and the third chamber, for example.
  • this further comprises a means for generating the electromagnetic force.
  • the means is part of the cartridge, the agent can be matched to a respective type of cartridge and securely connected to the corresponding cartridge.
  • the means may be formed as a coil or permanent magnet.
  • the means is integrated in a housing of the cartridge, in the first, second and / or third drum.
  • the agent can be easily arranged in the mixing chamber.
  • the mixing chamber comprises a flexible membrane, which divides the container into a first and a second volume, wherein in the first volume, the at least one first and second component and in the second volume, the electromagnetic particles can be received are, by means of the electromagnetic particles under the action of the electromagnetic force, the membrane is deformable to mix the at least one first and second component.
  • the particles are separated at any time from the first and second components, so that the particles can also be formed from, for example, non-sterile materials.
  • the cartridge according to the invention further comprises the first and second components and the electromagnetic particles, wherein the first component is designed as a biochemical probe and the second component as a capture molecule, which binds the biochemical probe, wherein the electromagnetic particles, the first or second Wear component.
  • the catcher molecules bind to the biochemical probes within a very short time.
  • the at least one means is adapted to generate an electromagnetic force which acts counter to the centrifugal force, perpendicular to the centrifugal force and / or in the same direction as the centrifugal force.
  • At least a first and a second means are provided, wherein the first means on one side and the second means on the other side of a circular path along which the cartridge is movable during centrifugation, is arranged, and the first and second means are spaced apart along the circular path.
  • the particles can be easily reciprocated in a direction perpendicular to the plane of the circular path.
  • the at least one means in a housing of the centrifuge in particular in a bottom and / or cover thereof, integrated. This results in a simple structure.
  • the electromagnetic force changes while the cartridge is being centrifuged.
  • the electromagnetic force may change with respect to its magnitude and / or direction.
  • FIG. 1 shows in a sectional view a cartridge 100 according to an embodiment of the present invention.
  • the cartridge 100 includes a housing 102 in the form of a tube.
  • the housing 102 may be a 15 mL centrifuge tube, 1.5 mL, or 2 mL
  • Eppendorf tube or alternatively as a microtiter plate (e.g., 20 pL per well).
  • the longitudinal axis of the housing 102 is designated 104.
  • the spring 114 is disposed between the shutter 118 and the drum 106 so that the spring 114 is stretched to generate a restoring force.
  • Other arrangements of the spring 114 are conceivable.
  • the second drum 106 includes a plurality of reagent chambers 120 and another chamber 122 for receiving a sample, such as a blood sample, taken from a patient.
  • a sample such as a blood sample
  • FIGS. 3A and 4A show a first position in which the projections 240 of the first drum 108 engage in the slots 204 and thus a rotation of the drum 108 is locked about the longitudinal axis 104. If the rotational speed of the centrifuge is increased, then the second drum 106 pushes, by means of the bevels 220 of the contour 216, onto the bevels 242 of the first drum 108 against the action of the spring 114, compressing the spring 114. As a result, the first drum 108 moves in a direction away from the pivot point 140 as indicated by the corresponding arrows in FIGS FIGS. 4A and 4B indicated. This movement is continued until the protrusions 240 disengage from the protrusions 200.
  • the spring 114 presses the first drum 108 by means of the third drum 110 again in the direction of the pivot point 140.
  • the second drum 106 together with their bevels 220 also back in Direction of the pivot point 140 moves, whereby the bevels 242 of the first drum 108 come to rest against the bevels 206 of the housing 102 and slide along this while performing a further rotational movement of the first drum 108 in a third position, as in Figures 4D and 4E shown.
  • the projections 240 of the first drum 108 are again located in the slots 204 of the housing 102, so that further rotation of the drum 108 about the longitudinal axis 104 is again locked.
  • the process described above may be repeated as many times as desired to rotate the first drum 108 in a defined manner relative to the other drums 106 and 110.
  • FIG. 5 shows in a section of the mixing chamber 124 from FIG. 1 according to an embodiment of the present invention.
  • the mixing chamber 124 comprises a container 500 for receiving at least two components 502, 504. These are preferably those components which are provided by means of the second drum 106, for example by means of one or more of the chambers 120, 122.
  • the components 502, 504 may be designed as reagents or samples, in particular blood samples.
  • FIG. 5 shows the container 500 as it contains a mixture of two liquids 502, 504.
  • the liquids 502, 504 may have the same or a different density.
  • the volume of liquid that can be received in the container 500 is typically up to 3 mL.
  • the mixing chamber 124 further comprises magnetic particles 506.
  • the particles 506 may already be arranged in the mixing chamber 124 before the start of the centrifuging, for example, even before the cartridge 100 is inserted into the centrifuge.
  • the particles 506 may be held in one or more chambers 120, 122 of the second drum 106 and initiated in a speed controlled manner at some time. In that regard, reference is made to the preceding, where the operation of the adjusting device 300 is described.
  • the particles 506 may be held in one or more of the chambers 120, 122 together with one of the liquids 502, 504, and later communicated together into the mixing chamber 124 (eg, the liquid 502 with the particles 506) by means of the adjustment device 300.
  • the particles 506 may be delivered from the chamber 120 before or after a blood sample has been introduced from the chamber 122 into the mixing chamber 124.
  • the particles 506 preferably have a permanent magnetic material, for example iron. More preferably, the particles 506 have a density which is greater than that of the liquids 502, 504. The particles 506 typically have a diameter of about 200 ⁇ m.
  • the centrifuge or cartridge 100 has a means 508 for generating a magnetic force 510 which acts on a respective particle 506.
  • the means 508 is preferably designed as a permanent magnet, coil or capacitor.
  • the means 508 is arranged in the present embodiment radially inward with respect to the mixing chamber 124 and the cartridge 100, respectively. An arrangement of the means 508 radially outward would be possible.
  • the generated magnetic force 510 preferably varies with time. This can be achieved, for example, once by the means 508 generating a temporally varying magnetic field.
  • the means 508 is designed, for example, as a coil, which is suitably energized by means of a control device.
  • the coil 508 may be a stationary part of the centrifuge.
  • the coil 508 may be moved, for example, integrated into the rotor of the centrifuge, into a special rotor holder, into the cartridge 100, into the first drum 108 or into the container 500, and in particular powered wirelessly.
  • the means 508 may be configured to generate a temporally constant magnetic field, wherein the mixing chamber 124 together with the particles 506 is moved relative thereto.
  • the means 508 is for example as Permanent magnet formed. In this case, the means 508 deflects the particles 506 whenever the cartridge 100 passes the means 508 in its orbit.
  • the means 508 is formed as a coil.
  • the magnetic force generated by the coil 508 by means of the control device acts against the centrifugal force 142 and exceeds it, for example, periodically.
  • the particles 506 reciprocate along the longitudinal axis 104 of the cartridge 100 in the liquids 502, 504, which is because of adjusting flows also leads to a movement of the particles 506 in a direction transverse to the longitudinal axis 104.
  • the movement of the particles 508 can also be controlled exclusively by means of the coil 508. That is, the coil 508 generates a magnetic force 510 that acts alternately against the centrifugal force 142 and in the direction of the centrifugal force 142. In this case, the density of the particles 506 as well as the strength of the centrifugal force 142 plays no role or only an insignificant role.
  • the method described can also be used to accelerate biochemical binding processes.
  • the mixing chamber 124 is then at least one liquid 502 containing at least one type of biochemical probe (e.g., DNA, antigens, antibodies, proteins, cells, gene sequences, amino acids).
  • a type of capture molecule e.g., DNA, antigens, antibodies, proteins, cells, gene sequences, amino acids
  • an accelerated loading of the particles 506 with the biochemical probes takes place.
  • liquids 502, 504 and the binding of biochemical probes to the surface of a respective particle 506 take place in one process step.
  • FIG. 6A shows a schematic plan view of a centrifuge 600 according to an embodiment of the present invention.
  • FIG. 6B schematically shows a side view of the centrifuge 600.
  • the cartridge 100 is - except for the mixing chamber 124 - for simplicity in FIG. 6B not shown.
  • the cartridge 100 together with the chamber 124 is moved in a holder, not shown, of the centrifuge 600 on a circular path 602 about the pivot point 140.
  • the centrifuge 600 has two permanent magnets 508, which are provided in each case stationary and for the sake of better distinction, designated by the reference numerals 604, 606.
  • the one permanent magnet 604 below the circular path 602 and the other permanent magnet 606 above the circular path 602 is arranged.
  • the lower permanent magnet 604 is integrated into a bottom 608 of the centrifuge 600 and the upper permanent magnet 606 is integrated into a cover 610 of the centrifuge 600.
  • the permanent magnets 604, 606 along the circular path 602 in the plan view from FIG. 6A offset from each other.
  • the permanent magnets 604, 606 are in relation to the pivot point 140 opposite.
  • FIGS. 7A and 7B show a variant over the embodiment according to FIG. 5 , where two different states are shown.
  • the mixing chamber 124 comprises a flexible, in particular elastic membrane 700 made of, for example, silicone, thermoplastic elastomer or polyamide.
  • the membrane 700 divides the container 500 into a first volume 702 and a second volume 704.
  • the second volume 704 contains the particles 506 and optionally one Gas 706.
  • the particles 506 may also be provided as a so-called ferrofluid.
  • FIG. 7A shows a first state in which the magnetic force 510 acts on a respective particle 506, and
  • FIG. 7B shows a second state in which no magnetic force 510 and only the centrifugal force 142 acts on a respective particle 506. In the second state, it is the first and second liquids 502, 504 that deform the membrane 700.
  • the rotational speed of the centrifuge can thus be mixed in all the above embodiments.
  • the rotational speed of the centrifuge may be selected such that the corresponding centrifugal force 142 exceeds a predetermined threshold, so that the adjustment device 300 rotates the drum 108 with the mixing chamber 124, whereby the chamber 124 with another chamber 120, 122, 132, 136 is conductively connected.
  • the particles 506 are already moving in the container 500 and may thereby mix the components 502, 504 together while one or both components 502, 504 flow into or out of the mixing chamber 124.
  • the embodiments described herein are combined.
  • this provides for the embodiments according to the FIG. 5 and Figures 6A and 6B such as FIGS. 7A and 7B and Figures 6A and 6B at.
  • the particles 506 are then reciprocated in the longitudinal direction 104 and perpendicular thereto for each full revolution of the cartridge 100.
  • the mixing chamber 124 may comprise an obstacle structure, not shown, for example, a sieve or a grid structure, which is adapted, by the action of a centrifugal force (ie when the rotational speed of the centrifuge exceeds a predetermined threshold) through the liquids 502, 504 to move or to be flowed around by the liquids 502, 504 (the latter in the case of a stationary obstacle structure), thereby to mix them.
  • an obstacle structure not shown, for example, a sieve or a grid structure, which is adapted, by the action of a centrifugal force (ie when the rotational speed of the centrifuge exceeds a predetermined threshold) through the liquids 502, 504 to move or to be flowed around by the liquids 502, 504 (the latter in the case of a stationary obstacle structure), thereby to mix them.
  • the housing 102 and the drums 106, 108, 110 may be made of the same or different polymers.
  • the one or more polymers are, in particular, thermoplastics, elastomers or thermoplastic elastomers. Examples are cycloolefin polymer (COP), cycloolefin copolymer (COC), polycarbonates (PC), polyamides (PA), polyurethanes (PU), polypropylene (PP), polyethylene terephthalate (PET) or poly (methyl methacrylate) ( PMMA).
  • COP cycloolefin polymer
  • COC cycloolefin copolymer
  • PC polycarbonates
  • PA polyamides
  • PU polyurethanes
  • PP polypropylene
  • PET polyethylene terephthalate
  • PMMA poly (methyl methacrylate)
  • One or both drums 106, 110 may be formed integrally with the housing 102.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Centrifugal Separators (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)
EP12163532.0A 2011-06-07 2012-04-10 Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant Not-in-force EP2532428B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102011077134A DE102011077134A1 (de) 2011-06-07 2011-06-07 Kartusche, Zentrifuge sowie Verfahren zum Mischen einer ersten und zweiten Komponente

Publications (3)

Publication Number Publication Date
EP2532428A2 true EP2532428A2 (fr) 2012-12-12
EP2532428A3 EP2532428A3 (fr) 2013-04-03
EP2532428B1 EP2532428B1 (fr) 2014-07-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP12163532.0A Not-in-force EP2532428B1 (fr) 2011-06-07 2012-04-10 Cartouche, centrifugeuse ainsi que procédé de mélange d'un premier et d'un deuxième composant

Country Status (4)

Country Link
US (1) US9475043B2 (fr)
EP (1) EP2532428B1 (fr)
CN (1) CN102814240B (fr)
DE (1) DE102011077134A1 (fr)

Cited By (5)

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Publication number Priority date Publication date Assignee Title
EP2532426A3 (fr) * 2011-06-07 2013-03-27 Robert Bosch Gmbh Cartouche, centrifugeuse et procédé
EP2532427A3 (fr) * 2011-06-07 2013-03-27 Robert Bosch Gmbh Cartouche, centrifugeuse et procédé
WO2014117908A1 (fr) * 2013-01-30 2014-08-07 Robert Bosch Gmbh Dispositif d'extraction de liquides organiques préstockés à sec dans un prélèvement, cartouche et procédé
EP2774676A1 (fr) * 2013-03-05 2014-09-10 Robert Bosch Gmbh Procédé et dispositif de couplage fluidique de cavités dans une centrifugeuse
WO2015049074A1 (fr) * 2013-10-02 2015-04-09 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Dispositif et procédé pour déplacer une phase solide dans une pluralité de chambres

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US20140100102A1 (en) * 2012-10-10 2014-04-10 California Institute Of Technology Devices and methods for cell lysis and sample preparation through centrifugation
US20140135198A1 (en) * 2012-11-12 2014-05-15 California Institute Of Technology Devices and methods for nucleic acid extraction capture and concentration using electric fields and centrifugation
US20160053216A1 (en) * 2013-04-03 2016-02-25 Sanko Ozel Egitim Hizmetleri A.S. A machine enabling separation of the blood by magnetism and autonomous radioactive substance marking of the white blood cells
DE102013222283B3 (de) 2013-11-04 2015-01-15 Robert Bosch Gmbh Vorrichtung und Verfahren zur Handhabung von Reagenzien
KR101472821B1 (ko) * 2014-05-14 2014-12-16 김준우 성분 분리기
US11918997B2 (en) * 2018-09-21 2024-03-05 Foss Analytical A/S Sampling device, a system comprising the sampling device and a method

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Also Published As

Publication number Publication date
DE102011077134A1 (de) 2012-12-13
EP2532428B1 (fr) 2014-07-02
EP2532428A3 (fr) 2013-04-03
US20120314531A1 (en) 2012-12-13
CN102814240A (zh) 2012-12-12
US9475043B2 (en) 2016-10-25
CN102814240B (zh) 2016-12-14

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