WO2012087039A3 - 호모세린 아세틸 트랜스퍼라제 활성을 가지는 변이형 폴리펩티드 및 이를 발현하는 미생물 - Google Patents

호모세린 아세틸 트랜스퍼라제 활성을 가지는 변이형 폴리펩티드 및 이를 발현하는 미생물 Download PDF

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WO2012087039A3
WO2012087039A3 PCT/KR2011/009966 KR2011009966W WO2012087039A3 WO 2012087039 A3 WO2012087039 A3 WO 2012087039A3 KR 2011009966 W KR2011009966 W KR 2011009966W WO 2012087039 A3 WO2012087039 A3 WO 2012087039A3
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WIPO (PCT)
Prior art keywords
acetyltransferase activity
variant polypeptide
homoserine acetyltransferase
microorganism expressing
expressing same
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PCT/KR2011/009966
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English (en)
French (fr)
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WO2012087039A2 (ko
Inventor
김소영
신용욱
서창일
허인경
김주은
김현아
이한진
나광호
손성광
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씨제이제일제당 (주)
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Priority to BR112013015991A priority Critical patent/BR112013015991A2/pt
Priority to JP2013546021A priority patent/JP5758499B2/ja
Priority to PL11852010T priority patent/PL2657250T3/pl
Priority to RU2013133626/10A priority patent/RU2557411C2/ru
Priority to ES11852010.5T priority patent/ES2650451T3/es
Priority to EP11852010.5A priority patent/EP2657250B1/en
Priority to US13/996,990 priority patent/US9127257B2/en
Priority to DK11852010.5T priority patent/DK2657250T3/en
Priority to NO11852010A priority patent/NO2657250T3/no
Priority to CN201180065592.XA priority patent/CN103797027B/zh
Publication of WO2012087039A2 publication Critical patent/WO2012087039A2/ko
Publication of WO2012087039A3 publication Critical patent/WO2012087039A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01031—Homoserine O-acetyltransferase (2.3.1.31)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 호모세린 O-아세틸 트랜스퍼라제 활성을 가지도록 변형시킨 폴리펩티드에 관한 것으로, 구체적으로 호모세린 숙시닐 트랜스퍼라제 활성을 가지는 폴리펩티드의 시작 메치오닌으로부터 111번째 아미노산을 치환시킴으로써 호모세린 O-아세틸 트랜스퍼라제 활성을 가지는 변이형 폴리펩티드를 제공한다.
PCT/KR2011/009966 2010-12-21 2011-12-21 호모세린 아세틸 트랜스퍼라제 활성을 가지는 변이형 폴리펩티드 및 이를 발현하는 미생물 WO2012087039A2 (ko)

Priority Applications (10)

Application Number Priority Date Filing Date Title
BR112013015991A BR112013015991A2 (pt) 2010-12-21 2011-12-21 polipeptídeos variante tendo homosserina atividade acetiltransferase e microorganismos expressando o mesmo
JP2013546021A JP5758499B2 (ja) 2010-12-21 2011-12-21 ホモセリンアセチルトランスフェラーゼ活性を有する変異型ポリペプチド及びそれを発現する微生物
PL11852010T PL2657250T3 (pl) 2010-12-21 2011-12-21 Odmiana polipeptydu posiadającego aktywność acetylotransferazy homoseryny oraz mikroorganizm wyrażający to samo
RU2013133626/10A RU2557411C2 (ru) 2010-12-21 2011-12-21 Модифицированный полипептид, обдадающий гомосеринацетилтрансферазной активностью, и экспрессирующий его микроорганизм
ES11852010.5T ES2650451T3 (es) 2010-12-21 2011-12-21 Variante de polipéptido que tiene actividad homoserina acetiltransferasa y microorganismo que expresa el mismo
EP11852010.5A EP2657250B1 (en) 2010-12-21 2011-12-21 Variant polypeptide having homoserine acetyltransferase activity and microorganism expressing same
US13/996,990 US9127257B2 (en) 2010-12-21 2011-12-21 Modified polypeptide having homoserine acetyltransferase activity and microorganism expressing the same
DK11852010.5T DK2657250T3 (en) 2010-12-21 2011-12-21 Polypeptide variant having homoserine acetyl transferase activity and microorganism expressing this
NO11852010A NO2657250T3 (ko) 2010-12-21 2011-12-21
CN201180065592.XA CN103797027B (zh) 2010-12-21 2011-12-21 具有高丝氨酸乙酰转移酶活性的修饰多肽和表达其的微生物

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20100131953 2010-12-21
KR10-2010-0131953 2010-12-21
KR1020110139228A KR101335841B1 (ko) 2010-12-21 2011-12-21 호모세린 아세틸 트랜스퍼라제 활성을 가지는 변이형 폴리펩티드 및 이를 발현하는 미생물
KR10-2011-0139228 2011-12-21

Publications (2)

Publication Number Publication Date
WO2012087039A2 WO2012087039A2 (ko) 2012-06-28
WO2012087039A3 true WO2012087039A3 (ko) 2012-08-23

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Application Number Title Priority Date Filing Date
PCT/KR2011/009966 WO2012087039A2 (ko) 2010-12-21 2011-12-21 호모세린 아세틸 트랜스퍼라제 활성을 가지는 변이형 폴리펩티드 및 이를 발현하는 미생물

Country Status (13)

Country Link
US (1) US9127257B2 (ko)
EP (1) EP2657250B1 (ko)
JP (1) JP5758499B2 (ko)
KR (1) KR101335841B1 (ko)
CN (1) CN103797027B (ko)
BR (1) BR112013015991A2 (ko)
DK (1) DK2657250T3 (ko)
ES (1) ES2650451T3 (ko)
MY (1) MY173933A (ko)
NO (1) NO2657250T3 (ko)
PL (1) PL2657250T3 (ko)
RU (1) RU2557411C2 (ko)
WO (1) WO2012087039A2 (ko)

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KR101825777B1 (ko) 2014-06-05 2018-02-07 씨제이제일제당 (주) O-아세틸-호모세린을 생산하는 미생물 및 이를 이용하여 o-아세틸-호모세린을 생산하는 방법
KR101641770B1 (ko) 2014-06-23 2016-07-22 씨제이제일제당 (주) O-아세틸 호모세린을 생산하는 미생물 및 상기 미생물을 이용하여 o-아세틸 호모세린을 생산하는 방법
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RU2747493C1 (ru) * 2017-06-30 2021-05-05 СиДжей ЧеилДжеданг Корпорейшн Новый вариант О-сукцинилгомосеринтрансферазы и способ получения О-сукцинилгомосерина с использованием этого варианта
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US10889806B2 (en) 2018-07-09 2021-01-12 Codexis, Inc. Engineered pantothenate kinase variant enzymes
KR101996769B1 (ko) 2018-12-21 2019-10-01 씨제이제일제당 (주) 변이형 호모세린 디하이드로게나제 및 이를 이용한 호모세린 또는 호모세린 유래 l-아미노산의 생산 방법
BR112023020035A2 (pt) 2021-04-01 2023-11-14 Evonik Operations Gmbh Método enzimático para produzir l-glufosinato e seus fosfoésteres
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Also Published As

Publication number Publication date
WO2012087039A2 (ko) 2012-06-28
CN103797027A (zh) 2014-05-14
CN103797027B (zh) 2017-05-17
NO2657250T3 (ko) 2018-02-03
RU2557411C2 (ru) 2015-07-20
JP5758499B2 (ja) 2015-08-05
JP2014502843A (ja) 2014-02-06
EP2657250A4 (en) 2015-02-18
MY173933A (en) 2020-02-27
ES2650451T3 (es) 2018-01-18
DK2657250T3 (en) 2017-12-11
EP2657250A2 (en) 2013-10-30
KR20120070531A (ko) 2012-06-29
US20130273615A1 (en) 2013-10-17
BR112013015991A2 (pt) 2018-07-10
RU2013133626A (ru) 2015-01-27
KR101335841B1 (ko) 2013-12-02
PL2657250T3 (pl) 2018-02-28
US9127257B2 (en) 2015-09-08
EP2657250B1 (en) 2017-09-06

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