WO2011070791A1 - 細胞または微生物の培養方法 - Google Patents

細胞または微生物の培養方法 Download PDF

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Publication number
WO2011070791A1
WO2011070791A1 PCT/JP2010/007202 JP2010007202W WO2011070791A1 WO 2011070791 A1 WO2011070791 A1 WO 2011070791A1 JP 2010007202 W JP2010007202 W JP 2010007202W WO 2011070791 A1 WO2011070791 A1 WO 2011070791A1
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WIPO (PCT)
Prior art keywords
culture solution
culture
oxygen
porous body
air
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Application number
PCT/JP2010/007202
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English (en)
French (fr)
Japanese (ja)
Inventor
大成 奥村
守 沼田
秀藏 小島
靖久 長田
直樹 田原
奉至 黒木
久木崎　雅人
智博 田中
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日揮株式会社
宮崎県
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Publication date
Application filed by 日揮株式会社, 宮崎県 filed Critical 日揮株式会社
Priority to EP10835714.6A priority Critical patent/EP2511364A4/en
Priority to CN201080056283.1A priority patent/CN102725392B/zh
Priority to KR1020147022329A priority patent/KR20140101886A/ko
Priority to JP2011545095A priority patent/JPWO2011070791A1/ja
Publication of WO2011070791A1 publication Critical patent/WO2011070791A1/ja
Priority to US13/485,085 priority patent/US20120244602A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B01F23/23105—Arrangement or manipulation of the gas bubbling devices
    • B01F23/2311—Mounting the bubbling devices or the diffusers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
    • B01F23/23105—Arrangement or manipulation of the gas bubbling devices
    • B01F23/2312—Diffusers
    • B01F23/23123—Diffusers consisting of rigid porous or perforated material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/111—Centrifugal stirrers, i.e. stirrers with radial outlets; Stirrers of the turbine type, e.g. with means to guide the flow
    • B01F27/1111—Centrifugal stirrers, i.e. stirrers with radial outlets; Stirrers of the turbine type, e.g. with means to guide the flow with a flat disc or with a disc-like element equipped with blades, e.g. Rushton turbine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/81—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis the stirrers having central axial inflow and substantially radial outflow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
PCT/JP2010/007202 2009-12-10 2010-12-10 細胞または微生物の培養方法 WO2011070791A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP10835714.6A EP2511364A4 (en) 2009-12-10 2010-12-10 METHOD FOR CULTURING CELLS OR MICRO-ORGANISMS
CN201080056283.1A CN102725392B (zh) 2009-12-10 2010-12-10 细胞或微生物的培养方法
KR1020147022329A KR20140101886A (ko) 2009-12-10 2010-12-10 세포 또는 미생물의 배양 방법
JP2011545095A JPWO2011070791A1 (ja) 2009-12-10 2010-12-10 細胞または微生物の培養方法
US13/485,085 US20120244602A1 (en) 2009-12-10 2012-05-31 Method for culturing cells or microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009-280842 2009-12-10
JP2009280842 2009-12-10

Related Child Applications (1)

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US13/485,085 Continuation US20120244602A1 (en) 2009-12-10 2012-05-31 Method for culturing cells or microorganisms

Publications (1)

Publication Number Publication Date
WO2011070791A1 true WO2011070791A1 (ja) 2011-06-16

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PCT/JP2010/007202 WO2011070791A1 (ja) 2009-12-10 2010-12-10 細胞または微生物の培養方法

Country Status (7)

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US (1) US20120244602A1 (ko)
EP (1) EP2511364A4 (ko)
JP (1) JPWO2011070791A1 (ko)
KR (2) KR20120104219A (ko)
CN (1) CN102725392B (ko)
TW (1) TW201129698A (ko)
WO (1) WO2011070791A1 (ko)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150036311A (ko) * 2012-07-02 2015-04-07 사노피 파스퇴르 헤모필루스 인플루엔자에 비형 항원의 생산 방법
WO2016002492A1 (ja) * 2014-07-01 2016-01-07 佐竹化学機械工業株式会社 微細気泡発生装置を有する往復動撹拌装置
WO2020003833A1 (ja) 2018-06-27 2020-01-02 富士フイルム株式会社 細胞培養方法、生産物の製造方法、及び細胞培養装置

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3659627T3 (da) * 2013-03-08 2024-04-02 Univ California Polyphosphat-funktionaliserede uorganiske nanopartikler som hæmostatiske sammensætninger og anvendelsesfremgangsmåder
KR102162400B1 (ko) * 2016-05-13 2020-10-06 시그마 테크놀로지 유겐가이샤 생체 투여 가능한 수용액 및 그 제조 방법

Citations (2)

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Publication number Priority date Publication date Assignee Title
JP2005169359A (ja) 2003-12-15 2005-06-30 Miyazaki Prefecture 単分散気泡の生成方法
JP2009018296A (ja) * 2007-07-12 2009-01-29 Spg Trading Kk Ｓｐｇ（シラス多孔質ガラス）酸素水製造機

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US4264739A (en) * 1979-01-05 1981-04-28 Merck & Co., Inc. Sparger for cell culture system
JPS6140841A (ja) * 1984-07-31 1986-02-27 Miyazakiken 多孔質ガラス成形物及びその製造方法

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
JP2005169359A (ja) 2003-12-15 2005-06-30 Miyazaki Prefecture 単分散気泡の生成方法
JP2009018296A (ja) * 2007-07-12 2009-01-29 Spg Trading Kk Ｓｐｇ（シラス多孔質ガラス）酸素水製造機

Non-Patent Citations (3)

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Title
DIRK NEHRING ET AL: "Experimental study of a ceramic microsparging aeration system in a pliot-scale animal cellculture", BIOTECHNOL. PROG., vol. 20, no. 6, 2004, pages 1710 - 1717, XP008157141 *
S.ZHANG ET AL: "Oxygen transfer properties of bubbles in animal cell culture media", BIOTECHNOLOGY AND BIOENGINEERING, vol. 40, no. 2, 1992, pages 252 - 259, XP008157146 *
See also references of EP2511364A4

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150036311A (ko) * 2012-07-02 2015-04-07 사노피 파스퇴르 헤모필루스 인플루엔자에 비형 항원의 생산 방법
JP2015522272A (ja) * 2012-07-02 2015-08-06 サノフィ・パスツールＳａｎｏｆｉｐａｓｔｅｕｒ ヘモフィルス・インフルエンザｂ型菌抗原の生成方法
KR102051482B1 (ko) 2012-07-02 2019-12-03 사노피 파스퇴르 에스에이 헤모필루스 인플루엔자에 비형 항원의 생산 방법
WO2016002492A1 (ja) * 2014-07-01 2016-01-07 佐竹化学機械工業株式会社 微細気泡発生装置を有する往復動撹拌装置
JPWO2016002492A1 (ja) * 2014-07-01 2017-04-27 佐竹化学機械工業株式会社 微細気泡発生装置を有する往復動撹拌装置
TWI664013B (zh) * 2014-07-01 2019-07-01 Satake Chemical Equipment Mfg Ltd. 具有微細氣泡產生裝置之往復移動攪拌裝置
US10799841B2 (en) 2014-07-01 2020-10-13 Satake Chemical Equipment Mfg Ltd. Reciprocating stirring apparatus having micro bubble generating device
WO2020003833A1 (ja) 2018-06-27 2020-01-02 富士フイルム株式会社 細胞培養方法、生産物の製造方法、及び細胞培養装置

Also Published As

Publication number Publication date
CN102725392B (zh) 2014-04-16
CN102725392A (zh) 2012-10-10
EP2511364A9 (en) 2013-04-17
KR20140101886A (ko) 2014-08-20
EP2511364A4 (en) 2013-10-16
KR20120104219A (ko) 2012-09-20
EP2511364A1 (en) 2012-10-17
US20120244602A1 (en) 2012-09-27
TW201129698A (en) 2011-09-01
JPWO2011070791A1 (ja) 2013-04-22

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