WO2011052946A3 - 재생성 세포 추출 시스템 - Google Patents

재생성 세포 추출 시스템 Download PDF

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Publication number
WO2011052946A3
WO2011052946A3 PCT/KR2010/007339 KR2010007339W WO2011052946A3 WO 2011052946 A3 WO2011052946 A3 WO 2011052946A3 KR 2010007339 W KR2010007339 W KR 2010007339W WO 2011052946 A3 WO2011052946 A3 WO 2011052946A3
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unit
tissues
cells
regenerative
extraction system
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PCT/KR2010/007339
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French (fr)
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WO2011052946A2 (ko
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도병록
이정규
김지향
강성구
박승훈
김명진
신규철
김철근
윤용달
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주식회사 퓨어바이오텍
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Priority to CN2010800538086A priority Critical patent/CN102869761A/zh
Priority to JP2012536661A priority patent/JP5826183B2/ja
Publication of WO2011052946A2 publication Critical patent/WO2011052946A2/ko
Publication of WO2011052946A3 publication Critical patent/WO2011052946A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/24—Apparatus for enzymology or microbiology tube or bottle type
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/05—Means for pre-treatment of biological substances by centrifugation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • External Artificial Organs (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

재생성 세포 추출 시스템이 개시된다. 본 발명의 실시예에 따른 재생성 세포 추출 시스템은 제1유닛, 제2유닛 및 이송부를 구비한다. 제1유닛은 진공흡입에 의하여 조직을 채취하고, 교반 및 원심분리에 의해 채취된 상기 조직으로부터 혈액오염물을 분리시키고, 상기 혈액오염물이 분리된 조직으로부터 세포를 분리하여 액체에 부유된 상태의 세포를 배출시킨다. 제2유닛은 상기 제1유닛으로부터 상기 액체에 부유된 상태의 세포를 투입받고, 원심분리에 의해서 재생성세포를 분리하며 상기 제1유닛보다 소용량이다. 이송부는 상기 제1유닛과 상기 제2유닛 사이에 연결되며, 상기 제1유닛과 상기 제2유닛에 세정수를 주입하고, 상기 제1유닛 및 상기 제2유닛으로부터의 추출물을 대응되는 백들로 전달하며, 상기 제1유닛에서 추출된 액체에 부유된 상태의 세포를 상기 제2유닛으로 투입시키거나 조직 및 세포오염물을 이송한다. 상기 조직은 지방조직이며, 상기 재생성세포는 지방유래줄기세포이다.
PCT/KR2010/007339 2009-10-27 2010-10-25 재생성 세포 추출 시스템 WO2011052946A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN2010800538086A CN102869761A (zh) 2009-10-27 2010-10-25 再生细胞提取系统
JP2012536661A JP5826183B2 (ja) 2009-10-27 2010-10-25 再生細胞抽出システム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090102082A KR101679671B1 (ko) 2009-10-27 2009-10-27 재생성 세포 추출 시스템
KR10-2009-0102082 2009-10-27

Publications (2)

Publication Number Publication Date
WO2011052946A2 WO2011052946A2 (ko) 2011-05-05
WO2011052946A3 true WO2011052946A3 (ko) 2011-11-03

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PCT/KR2010/007339 WO2011052946A2 (ko) 2009-10-27 2010-10-25 재생성 세포 추출 시스템

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Country Link
JP (1) JP5826183B2 (ko)
KR (1) KR101679671B1 (ko)
CN (1) CN102869761A (ko)
WO (1) WO2011052946A2 (ko)

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Also Published As

Publication number Publication date
CN102869761A (zh) 2013-01-09
KR20110045479A (ko) 2011-05-04
WO2011052946A2 (ko) 2011-05-05
JP5826183B2 (ja) 2015-12-02
JP2013507983A (ja) 2013-03-07
KR101679671B1 (ko) 2016-11-28

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