WO2011052948A3 - 재생성 세포 추출유닛 및 재생성 세포 추출시스템 - Google Patents

재생성 세포 추출유닛 및 재생성 세포 추출시스템 Download PDF

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Publication number
WO2011052948A3
WO2011052948A3 PCT/KR2010/007341 KR2010007341W WO2011052948A3 WO 2011052948 A3 WO2011052948 A3 WO 2011052948A3 KR 2010007341 W KR2010007341 W KR 2010007341W WO 2011052948 A3 WO2011052948 A3 WO 2011052948A3
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Prior art keywords
regenerative cell
cell extraction
extraction system
regenerative
container
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PCT/KR2010/007341
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English (en)
French (fr)
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WO2011052948A2 (ko
Inventor
도병록
이정규
김지향
강성구
박승훈
김명진
신규철
김철근
윤용달
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주식회사 퓨어바이오텍
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Priority to US13/504,586 priority Critical patent/US9388377B2/en
Priority to CN201080059536.0A priority patent/CN102741391B/zh
Priority to JP2012536662A priority patent/JP5923446B2/ja
Publication of WO2011052948A2 publication Critical patent/WO2011052948A2/ko
Publication of WO2011052948A3 publication Critical patent/WO2011052948A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/10—Apparatus for enzymology or microbiology rotatably mounted
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B11/00—Feeding, charging, or discharging bowls
    • B04B11/02—Continuous feeding or discharging; Control arrangements therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/05—Means for pre-treatment of biological substances by centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • Pathology (AREA)
  • Centrifugal Separators (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 재생성 세포 추출시스템은 제공한다. 본 발명에 따른 재생성 세포 추출시스템은, 지방조직(fat tissue)을 분리하기 위한 것으로서 외부로부터 회전력을 제공받아 회전되며 지방조직이 수용되는 서브 용기와, 서브 용기에 삽입되는 중공형의 출입관과, 출입관과 연결되어 펌프를 구비하는 재생성 세포 분리유닛을 구비하며, 또한 재생성 세포 분리유닛으로부터 원심분리되어 배출된 분리물을 이송받아 2차 원심분리를 통해 재생성 세포를 추출하기 위한 것으로서, 외부로부터 회전력을 제공받아 회전되는 메인 용기와, 메인 용기에 삽입되는 중공형의 제1배출관, 제1배출관에 연결되는 흡입장치를 구비하며, 분리대상물로부터 분리된 분리물들 중 상대적으로 무거운 분리물이 수용되도록 메인 용기의 회전축을 중심으로 하여 반경방향을 따라 외측으로 볼록하게 형성되는 복수의 돌출수용부가 형성되어 있는 재생성 세포 추출유닛을 구비한다.
PCT/KR2010/007341 2009-10-27 2010-10-25 재생성 세포 추출유닛 및 재생성 세포 추출시스템 WO2011052948A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/504,586 US9388377B2 (en) 2009-10-27 2010-10-25 Regenerative cell extraction unit and regenerative cell extraction system
CN201080059536.0A CN102741391B (zh) 2009-10-27 2010-10-25 再生细胞撷取单元与再生细胞撷取系统
JP2012536662A JP5923446B2 (ja) 2009-10-27 2010-10-25 再生細胞抽出ユニット及び再生細胞抽出システム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20090102085 2009-10-27
KR10-2009-0102085 2009-10-27

Publications (2)

Publication Number Publication Date
WO2011052948A2 WO2011052948A2 (ko) 2011-05-05
WO2011052948A3 true WO2011052948A3 (ko) 2011-10-06

Family

ID=43922789

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Application Number Title Priority Date Filing Date
PCT/KR2010/007341 WO2011052948A2 (ko) 2009-10-27 2010-10-25 재생성 세포 추출유닛 및 재생성 세포 추출시스템

Country Status (6)

Country Link
US (1) US9388377B2 (ko)
JP (1) JP5923446B2 (ko)
KR (2) KR101316573B1 (ko)
CN (1) CN102741391B (ko)
TW (1) TWI455751B (ko)
WO (1) WO2011052948A2 (ko)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9296984B2 (en) 2010-07-09 2016-03-29 The Gid Group, Inc. Tissue processing apparatus and method for processing adipose tissue
WO2013106655A1 (en) 2012-01-11 2013-07-18 The Gid Group, Inc. Method for processing adipose tissue and processing apparatus
US9206387B2 (en) 2010-07-09 2015-12-08 The Gid Group, Inc. Method and apparatus for processing adipose tissue
EP3366327B1 (en) 2010-07-09 2022-09-21 GID BIO, Inc. Apparatus and methods relating to collecting and processing human biological material containing adipose
AU2011343513B2 (en) * 2010-12-16 2016-07-14 Ingeneron, Inc. Methods and apparatus for enhanced recovery of cells and of cell-enriched matrix from tissue samples
AU2012335746B2 (en) 2011-11-08 2017-04-27 Auxocell Laboratories, Inc. Systems and methods for processing cells
KR101357160B1 (ko) * 2011-12-14 2014-02-17 주식회사 휴림바이오셀 재생성 세포 추출 시스템 및 이를 이용한 재생성 세포 추출 방법
BR112015004003B1 (pt) 2012-09-06 2020-05-19 The Gid Group Inc aparelho para processar material biológico humano contendo tecido fibroso e método para processar tecido adiposo
KR101466923B1 (ko) * 2013-01-17 2014-12-04 주식회사 휴림바이오셀 재생성 세포 추출장치, 재생성 세포 추출 시스템, 이를 이용한 재생성 세포 추출방법
KR101354959B1 (ko) * 2013-07-09 2014-01-22 김태영 줄기세포 분리기
WO2015035221A1 (en) 2013-09-05 2015-03-12 The Gid Group, Inc. Tissue processing apparatus and method for processing adipose tissue
KR101390920B1 (ko) * 2013-12-13 2014-04-30 김준우 지방파쇄기 및 지방파쇄가 가능한 성분분리기
WO2015167768A1 (en) 2014-05-02 2015-11-05 Lifecell Corporation Injection sensor with feedback mechanism
USD748462S1 (en) 2014-08-11 2016-02-02 Auxocell Laboratories, Inc. Centrifuge clip
US9993748B2 (en) 2014-08-11 2018-06-12 Auxocell Laboratories, Inc. Centrifuge clip and method
BR112018008050A2 (pt) 2015-10-21 2018-10-23 Lifecell Corp sistemas e métodos para gerenciamento de tubo
AU2016342012A1 (en) 2015-10-21 2018-05-10 Lifecell Corporation Systems and methods for medical device control
US10729827B2 (en) 2015-12-22 2020-08-04 Lifecell Corporation Syringe filling device for fat transfer
KR101736374B1 (ko) 2016-06-07 2017-05-17 (주)시지바이오 원심 분리기용 용기 및 이를 이용한 svf 분리 방법
WO2018044791A1 (en) 2016-08-30 2018-03-08 Lifecell Corporation Systems and methods for medical device control
USD851777S1 (en) 2017-01-30 2019-06-18 Lifecell Corporation Canister-type device for tissue processing
US20200115666A1 (en) * 2017-03-28 2020-04-16 Corning Incorporated Aseptic bioprocess package
TWI616526B (zh) * 2017-05-15 2018-03-01 Cell separation and purification device
EP3655518A4 (en) 2017-07-18 2021-07-14 GID Bio, Inc. ADAPTIVE TISSUE DIGESTION SYSTEM AND TISSUE PROCESSING PROCEDURES
CN109988700B (zh) * 2017-12-29 2023-11-17 湖南美柏生物医药有限公司 一种干细胞分离装置
CN109988706B (zh) * 2017-12-29 2022-10-18 肖锷 一种离心瓶及干细胞分类提取装置
JP7352146B2 (ja) * 2019-07-23 2023-09-28 住友ベークライト株式会社 分離器及び培養システム
US11148083B2 (en) * 2019-12-27 2021-10-19 Pall Corporation Method and system for recovering fluid
GB2587257B (en) * 2020-03-09 2021-09-15 Alma Lasers Ltd Lipoaspirate processing
CN113355233A (zh) * 2021-04-19 2021-09-07 杭州氢态生物科技有限公司 一种基于生物酶解靶向提取装置及提取工艺
KR102357075B1 (ko) 2021-09-28 2022-02-08 주식회사 이노브바이오 연골 추출 유닛
CN114644983B (zh) * 2022-03-21 2022-11-22 河南茵特赛尔生物技术有限公司 一种干细胞提取装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09276396A (ja) * 1996-04-03 1997-10-28 Haemonetics Corp 血液処理用遠心分離器ボウル
JP2001017540A (ja) * 1999-06-03 2001-01-23 Haemonetics Corp 血液処理遠心分離ボウルおよび全血から血漿分画を収集する方法
JP2006020756A (ja) * 2004-07-07 2006-01-26 Terumo Corp 遠心分離器および血液成分採取回路
JP2007236665A (ja) * 2006-03-09 2007-09-20 Asahi Kasei Corp 血液成分分離装置及びその使用方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0783707B2 (ja) * 1987-04-14 1995-09-13 東ソー株式会社 バイオリアクタ−
US4939087A (en) * 1987-05-12 1990-07-03 Washington State University Research Foundation, Inc. Method for continuous centrifugal bioprocessing
JPH05310781A (ja) * 1992-05-13 1993-11-22 Mitsui Toatsu Chem Inc Ｌ−α−アスパルチル−Ｌ−フェニルアラニンメチルエステルの改良された結晶製造法
JPH07231776A (ja) * 1994-02-24 1995-09-05 Kirin Eng Kk 発酵糖の製造装置
JPH09224923A (ja) * 1996-02-21 1997-09-02 Sekisui Chem Co Ltd 血清もしくは血漿分離用容器
US9144583B2 (en) * 2002-03-29 2015-09-29 Tissue Genesis, Inc. Cell separation apparatus and methods of use
WO2003086640A1 (en) * 2002-04-12 2003-10-23 Gambro, Inc. Fluid separation using a centrifuge and roller pump
CN101415451A (zh) * 2003-06-18 2009-04-22 马克罗珀尔生物外科公司 在加大自体脂肪转移时使用来自脂肪组织的细胞的方法
WO2005012480A2 (en) * 2003-06-25 2005-02-10 Macropore Biosurgery Inc. Systems and methods for separating and concentrating regenerative cells from tissue
FR2878426B1 (fr) * 2004-11-30 2007-03-02 Eric Peltier Flacon de preparation d'une suspension cytologique
US7364657B2 (en) * 2005-03-31 2008-04-29 Abbott Cardiovascular Systems Inc. Integrated system for on-site cell acquisition, processing, and delivery
JP2007282552A (ja) * 2006-04-14 2007-11-01 Biomaster Inc 細胞分離方法および細胞分離システム
KR100767448B1 (ko) * 2006-06-30 2007-10-17 메디칸(주) 원심분리기 및 원심분리방법
JP5078020B2 (ja) * 2008-02-13 2012-11-21 オリンパス株式会社 遠心分離容器

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09276396A (ja) * 1996-04-03 1997-10-28 Haemonetics Corp 血液処理用遠心分離器ボウル
JP2001017540A (ja) * 1999-06-03 2001-01-23 Haemonetics Corp 血液処理遠心分離ボウルおよび全血から血漿分画を収集する方法
JP2006020756A (ja) * 2004-07-07 2006-01-26 Terumo Corp 遠心分離器および血液成分採取回路
JP2007236665A (ja) * 2006-03-09 2007-09-20 Asahi Kasei Corp 血液成分分離装置及びその使用方法

Also Published As

Publication number Publication date
CN102741391B (zh) 2014-05-21
JP5923446B2 (ja) 2016-05-24
US9388377B2 (en) 2016-07-12
CN102741391A (zh) 2012-10-17
JP2013507984A (ja) 2013-03-07
KR20130006407A (ko) 2013-01-16
WO2011052948A2 (ko) 2011-05-05
US20120214659A1 (en) 2012-08-23
TW201119724A (en) 2011-06-16
KR101316573B1 (ko) 2013-10-15
KR101269906B1 (ko) 2013-05-31
KR20110046298A (ko) 2011-05-04
TWI455751B (zh) 2014-10-11

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