WO2011049329A3 - 지렁이의 생식소 재생능력을 이용한 형질전환 지렁이의 제조방법, 이에 의해 제조된 형질전환 지렁이, 및 형질전환 지렁이의 체액으로부터 재조합 단백질의 생산방법 - Google Patents

지렁이의 생식소 재생능력을 이용한 형질전환 지렁이의 제조방법, 이에 의해 제조된 형질전환 지렁이, 및 형질전환 지렁이의 체액으로부터 재조합 단백질의 생산방법 Download PDF

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WO2011049329A3
WO2011049329A3 PCT/KR2010/007078 KR2010007078W WO2011049329A3 WO 2011049329 A3 WO2011049329 A3 WO 2011049329A3 KR 2010007078 W KR2010007078 W KR 2010007078W WO 2011049329 A3 WO2011049329 A3 WO 2011049329A3
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Prior art keywords
transformed
earthworms
producing
present
earthworm
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PCT/KR2010/007078
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English (en)
French (fr)
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WO2011049329A2 (ko
WO2011049329A9 (ko
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김해경
안치현
탁은식
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(주)에코젠크래프트
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Priority to JP2012519484A priority Critical patent/JP5542928B2/ja
Priority to US13/384,766 priority patent/US8592207B2/en
Priority to CN2010800326947A priority patent/CN102482678B/zh
Publication of WO2011049329A2 publication Critical patent/WO2011049329A2/ko
Publication of WO2011049329A9 publication Critical patent/WO2011049329A9/ko
Publication of WO2011049329A3 publication Critical patent/WO2011049329A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0335—Genetically modified worms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/703—Worms, e.g. Caenorhabdities elegans

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 지렁이의 생식소 재생능력을 이용한 형질전환 지렁이의 제조방법, 이에 의해 제조된 형질전환 지렁이, 및 형질전환 지렁이의 체액으로부터 재조합 단백질의 생산방법에 관한 것이다. 본 발명에 따른 형질전환 지렁이의 제조방법은, 종래의 형질전환기술의 단점들을 보완하는 형태의 새로운 생명공학적 기법으로 높은 주입 효율성을 가지고, 배아줄기세포와 같은 전능성을 가지는 재생아세포를 사용함으로써 형질전환체의 모든 부위에 재조합 유전자를 삽입시킬 수 있어서 형질전환체의 체액을 통하여 재조합 단백질을 생산할 수 있으며, 생식소에 표적 유전자를 직접 투입함으로써 대리모(동물)가 필요없으며, 생식가능한 형질전환체를 이용하므로 지속적으로 다수의 형질전환체를 생산할 수 있고, 기존의 형질전환기술 보다 간편하며, 고가의 장비 및 숙련된 기술이 필요하지 않다.
PCT/KR2010/007078 2009-10-22 2010-10-15 지렁이의 생식소 재생능력을 이용한 형질전환 지렁이의 제조방법, 이에 의해 제조된 형질전환 지렁이, 및 형질전환 지렁이의 체액으로부터 재조합 단백질의 생산방법 WO2011049329A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2012519484A JP5542928B2 (ja) 2009-10-22 2010-10-15 ミミズの生殖巣再生能力を利用した形質転換ミミズの作製方法、これにより作製された形質転換ミミズ、及び形質転換ミミズの体液から組換たんぱく質の生産方法
US13/384,766 US8592207B2 (en) 2009-10-22 2010-10-15 Method for producing transformed earthworms using earthworm's gonad-regeneration capability, transformed earthworms thereof, and method for producing recombinant proteins from transformed earthworm body fluids
CN2010800326947A CN102482678B (zh) 2009-10-22 2010-10-15 利用蚯蚓的性腺再生能力的转基因蚯蚓的养殖方法，通过该养殖方法养殖的转基因蚯蚓以及从转基因蚯蚓的体液生产重组蛋白质的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0100907 2009-10-22
KR1020090100907A KR101235297B1 (ko) 2009-10-22 2009-10-22 지렁이의 생식소 재생능력을 이용한 형질전환 지렁이의 제조방법, 이에 의해 제조된 형질전환 지렁이, 및 형질전환 지렁이의 체액으로부터 재조합 단백질의 생산방법

Publications (3)

Publication Number Publication Date
WO2011049329A2 WO2011049329A2 (ko) 2011-04-28
WO2011049329A9 WO2011049329A9 (ko) 2011-07-21
WO2011049329A3 true WO2011049329A3 (ko) 2011-10-13

Family

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PCT/KR2010/007078 WO2011049329A2 (ko) 2009-10-22 2010-10-15 지렁이의 생식소 재생능력을 이용한 형질전환 지렁이의 제조방법, 이에 의해 제조된 형질전환 지렁이, 및 형질전환 지렁이의 체액으로부터 재조합 단백질의 생산방법

Country Status (5)

Country Link
US (1) US8592207B2 (ko)
JP (1) JP5542928B2 (ko)
KR (1) KR101235297B1 (ko)
CN (1) CN102482678B (ko)
WO (1) WO2011049329A2 (ko)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102288467B (zh) * 2011-07-20 2013-04-24 浙江工业大学 一种用于蛋白质组学分析的动物蛋白质样品的制备方法
JP6448294B2 (ja) * 2014-10-17 2019-01-09 独立行政法人国立高等専門学校機構 形質転換ミミズの作成方法及び組み換えタンパク質の生産方法及び組み換えタンパク質の回収方法
JP6692101B2 (ja) * 2018-11-30 2020-05-13 独立行政法人国立高等専門学校機構 形質転換ミミズの作成方法及び組み換えタンパク質の生産方法
JP7333930B2 (ja) * 2019-01-07 2023-08-28 独立行政法人国立高等専門学校機構 ミミズ細胞の保存方法及びミミズ培養細胞の形質転換方法
CN112679573A (zh) * 2021-01-21 2021-04-20 广东莞垄农业生态科技有限公司 一种地龙抗菌肽的制备方法及其应用
CN113087787B (zh) * 2021-05-19 2023-03-21 广西医科大学第二附属医院(广西医科大学第二临床医学院) 一种蚯蚓血红蛋白分离及纯化方法

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Also Published As

Publication number Publication date
WO2011049329A2 (ko) 2011-04-28
KR101235297B1 (ko) 2013-02-20
JP2012532604A (ja) 2012-12-20
CN102482678B (zh) 2013-07-17
US8592207B2 (en) 2013-11-26
WO2011049329A9 (ko) 2011-07-21
KR20110044087A (ko) 2011-04-28
US20120123100A1 (en) 2012-05-17
CN102482678A (zh) 2012-05-30
JP5542928B2 (ja) 2014-07-09

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