WO2010076849A1 - Appareil de culture - Google Patents

Appareil de culture Download PDF

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Publication number
WO2010076849A1
WO2010076849A1 PCT/JP2009/007267 JP2009007267W WO2010076849A1 WO 2010076849 A1 WO2010076849 A1 WO 2010076849A1 JP 2009007267 W JP2009007267 W JP 2009007267W WO 2010076849 A1 WO2010076849 A1 WO 2010076849A1
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WO
WIPO (PCT)
Prior art keywords
culture
chamber
door
vessel
culture vessel
Prior art date
Application number
PCT/JP2009/007267
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English (en)
Japanese (ja)
Inventor
越馬隆治
Original Assignee
株式会社ニコン
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Filing date
Publication date
Application filed by 株式会社ニコン filed Critical 株式会社ニコン
Publication of WO2010076849A1 publication Critical patent/WO2010076849A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Definitions

  • the present invention relates to a culture apparatus for culturing a sample.
  • a culture apparatus for culturing a sample such as a cell.
  • a culture apparatus for culturing a sample such as a cell.
  • a culture apparatus a plurality of culture containers in which a sample such as a cell is stored are stored in a culture chamber maintained at a predetermined temperature and humidity, and the sample is cultured.
  • automatic observation of a sample in a culture vessel is performed in a culture chamber.
  • the present invention has been made to solve such a conventional problem, and an object of the present invention is to provide a culture apparatus capable of preventing external light from entering the culture chamber when the sample is observed in the culture chamber. To do.
  • a culture apparatus includes a culture chamber in which a dark room maintained in a predetermined atmosphere is formed, in which a culture vessel is accommodated, and observation means for observing a sample in the culture vessel accommodated in the culture chamber
  • a first door provided in the culture chamber for carrying the culture vessel into or out of the culture chamber, or when the first door is opened.
  • a light shielding means for preventing external light from entering the vicinity of the observation means.
  • the culture apparatus is the culture apparatus according to the first aspect, wherein the light shielding means includes a light shielding box having a door for taking in and out the culture container and containing the observation means.
  • a culture apparatus is a culture chamber in which a dark room maintained in a predetermined atmosphere is formed, in which a culture container is accommodated, and an observation means for observing a sample in the culture container accommodated in the culture chamber
  • a first door that is provided in the culture chamber and that carries the culture vessel into or out of the culture chamber from the outside of the culture chamber, and when the first door is opened, the culture chamber
  • a light shielding means for preventing external light from entering the light.
  • the culture device of a fourth invention is the culture device of the first invention or the third invention, wherein the light shielding means is mounted on the outside of the first door of the culture chamber and is formed by a light shielding wall, An outer standby chamber for waiting for the culture vessel, a conveying means arranged in the culture chamber and carrying the culture vessel into or out of the outer standby chamber, and for bringing the culture vessel into or out of the outer standby chamber And a second door that is closed when the first door is opened and prevents external light from entering the culture chamber.
  • the culture device of the fifth invention is the culture device of the first invention or the third invention, wherein the light shielding means is disposed inside the first door of the culture chamber and is formed by a light shielding wall, An inner standby chamber for waiting the culture vessel; a conveying means disposed in the culture chamber; for carrying the culture vessel into or out of the inner standby chamber; provided in the conveying means; And a light shielding member that shields light from the inner standby chamber by contacting the inner standby chamber at the time of opening to prevent external light from entering the culture chamber.
  • the light shielding means is disposed inside the first door of the culture chamber and is formed by a light shielding wall, An inner standby chamber for waiting the culture vessel; a conveying means disposed in the culture chamber; for carrying the culture vessel into or out of the inner standby chamber; provided in the conveying means; And a light shielding member that shields light from the inner standby chamber by contacting the inner standby chamber at the time of opening to prevent external light from entering the culture chamber.
  • a culture apparatus is the culture apparatus according to the fifth aspect, wherein the inner standby chamber is extendable by a bellows member forming the light-shielding wall, and the culture vessel stands by outside the first door. It has the outside standby part for making it do.
  • a culture apparatus is a culture chamber in which a dark room maintained in a predetermined atmosphere is formed, in which a culture container is accommodated, and observation means for observing a sample in the culture container accommodated in the culture chamber A first door for bringing the culture vessel into or out of the culture chamber from the outside of the culture chamber, and from the observation schedule by the observation means into the culture chamber. And a control unit that obtains a time at which external light is allowed to enter and opens and closes the first door at the time allowed.
  • the culture apparatus of this embodiment has an upper casing 11 and a lower casing 13 as shown in FIG.
  • the upper casing 11 is placed on the lower casing 13.
  • a culture chamber 15 is formed in the upper casing 11.
  • the culture chamber 15 is surrounded by a light shielding wall so as to be a dark room, and the environment is maintained in a predetermined state, for example, an atmosphere of a temperature of 37 ° C., a humidity of 90%, and a carbon dioxide of 5%.
  • a stocker 19 In the culture chamber 15, a stocker 19, an illumination unit 21, an observation unit 23, and a transport robot 25 are arranged.
  • the stocker 19 has a plurality of accommodating portions 19a in the vertical and horizontal directions.
  • Each accommodation portion 19a can accommodate a culture container 31 such as a well plate in which cells are accommodated.
  • the illumination unit 21 has a light source such as an LED light source and an optical system.
  • the illumination unit 21 is housed in the housing and protrudes above the observation unit 23. Then, the observation unit 23 is illuminated.
  • the culture vessel 31 can be transported to the observation unit 23.
  • a microscope 33 is disposed in the observation unit 23.
  • the transport robot 25 transports the culture vessel 31 accommodated in the stocker 19 to the observation unit 23 and the outer standby unit 35 (described later).
  • the transfer robot 25 has a rotary stage 25b that rotates about a rotary shaft 25a.
  • a mini stage 25c is movably disposed on the rotary stage 25b.
  • An arm portion 25d is fixed to the mini stage 25c.
  • the arm portion 25d is composed of a pair of left and right arms, and the culture vessel 31 is placed on the arms and transported.
  • the rotary stage 25b is movable in the vertical direction by a screw shaft 25e rotated by a motor M1. Further, as shown in FIG. 1, it can be moved in the horizontal direction by a screw shaft 25f rotated by a motor M2.
  • a large door 37 and a middle door 39 are arranged on the front surface of the culture chamber 15.
  • the large door 37 and the middle door 39 are disposed so as to cover the front surface of the upper casing 11 as shown in FIG.
  • An operation unit 41 and a monitor 43 are disposed on the large door 37.
  • the operation unit 41 is for performing various operations of the culture apparatus, and is formed by a touch panel, for example.
  • the monitor 43 displays a sample image taken by a CCD camera 45 described later.
  • a first door 47 is disposed on the surface of the culture chamber 15 opposite to the large door 37.
  • the upper casing 11 is formed with a rectangular opening 11a for carrying the culture container 31 in and out.
  • a first door 47 is disposed so as to cover the opening 11a.
  • the first door 47 is movable in the vertical direction by the operation of the motor M3. As shown in FIG. 3, a pocket portion 47 a that accommodates the first door 47 is formed below the first door 47.
  • the opening / closing of the first door 47 can be detected by the first opening / closing sensor S1.
  • an outer standby chamber 49 for waiting for the culture vessel 31 is formed outside the first door 47 of the culture chamber 15.
  • the light shielding box 51 forming the outer standby chamber 49 is formed of a light shielding wall and has a rectangular shape.
  • the light shielding box 51 is fixed to the opening of the upper casing 11 so that light does not enter the inside.
  • an outer standby unit 35 for temporarily waiting for the culture vessel 31 carried into and out of the culture chamber 15 is disposed.
  • a presence / absence sensor S3 for determining the presence / absence of the culture vessel 31 is disposed.
  • a second door 53 is disposed at a position facing the first door 47.
  • the second door 53 can be opened and closed by driving the motor M4.
  • the second door 53 is opened and closed around the shaft 53a.
  • the opening / closing of the second door 53 can be detected by the second opening / closing sensor S2.
  • the lower part of the microscope 33 is extended.
  • a CCD camera 45 as an imaging unit is disposed at the lower end of the observation unit 23.
  • the image of the cells in the culture vessel 31 conveyed on the observation unit 23 is captured by the CCD camera 45 through the microscope 33 and displayed on the monitor 43.
  • a control device 55 is arranged in the lower casing 13.
  • the control device 55 includes a controller 57 and a control unit 59.
  • the control unit 59 controls the transport robot 25, the observation unit 23, and the like via the controller 57.
  • the control unit 59 includes a CPU 61 and a memory 63.
  • the memory 63 stores a schedule for imaging a sample by the CCD camera 45 and the like.
  • the culture container 31 is carried in and out as described below.
  • FIG. 4 is a flowchart showing the operation of bringing the culture vessel 31 into the culture chamber 15.
  • Step S1 The CPU 61 determines whether or not there has been an instruction to carry in the culture vessel 31.
  • the instruction to carry in the culture container 31 is performed by an operator operating the operation unit 41 to instruct the delivery of the culture container 31 and the position of the culture container 31 to be carried in the stocker 19.
  • Step S2 The CPU 61 drives the motor M4 to open the second door 53 of the light shielding box 51.
  • the opening of the second door 53 is detected by the second opening / closing sensor S2.
  • Step S3 The CPU 61 determines whether or not the culture vessel 31 is placed in the outer standby section 35 of the light shielding box 51. This determination is made by an input from the presence / absence sensor S3 of the outer standby unit 35. The culture vessel 31 is placed on the outer standby section 35 by the operator.
  • Step S4 The CPU 61 drives the motor M4 to close the second door 53 of the light shielding box 51.
  • the closing of the second door 53 is detected by the second opening / closing sensor S2.
  • Step S5 The CPU 61 drives the motor M3 to open the first door 47 of the culture chamber 15.
  • the opening of the first door 47 is detected by the first opening / closing sensor S1.
  • the second door 53 of the light shielding box 51 is closed, and no light from the outside enters the culture chamber 15.
  • Step S6 The CPU 61 drives the transfer robot 25 to transfer the culture vessel 31 placed in the outer standby unit 35 to a predetermined position of the stocker 19. More specifically, as shown in FIG. 5, the rotary stage 25b is positioned so that the arm portion 25d faces the first door 47, and in this state, the arm portion 25d is advanced into the outer standby portion 35 to culture the culture vessel. Pick up 31. Then, the culture container 31 is transported to the position instructed by the stocker 19.
  • Step S7 The CPU 61 drives the motor M3 to close the first door 47 of the culture chamber 15. The closing of the first door 47 is detected by the first opening / closing sensor S1.
  • FIG. 6 is a flowchart showing the operation of carrying out the culture vessel 31 from the culture chamber 15.
  • Step S1 The CPU 61 determines whether or not there is an instruction to carry out the culture vessel 31.
  • the instruction to carry out the culture vessel 31 is performed, for example, when the operator operates the operation unit 41 to instruct the position of the culture vessel 31 to be carried out and carried out in the stocker 19.
  • Step S2 The CPU 61 drives the motor M3 to open the first door 47 of the culture chamber 15. The opening of the first door 47 is detected by the first opening / closing sensor S1.
  • Step S3 The CPU 61 drives the transport robot 25 to transport the culture vessel 31 placed at a predetermined position of the stocker 19 to the outer standby unit 35. More specifically, as shown in FIG. 5, the rotary stage 25b is positioned so that the arm portion 25d faces the second door 53, and in this state, the arm portion 25d is advanced into the outer standby portion 35 to culture the culture vessel. 31 is placed on the outer standby section 35.
  • Step S4 The CPU 61 drives the motor M3 to close the first door 47 of the culture chamber 15. The closing of the first door 47 is detected by the first opening / closing sensor S1.
  • Step S5 The CPU 61 drives the motor M4 to open the second door 53 of the light shielding box 51.
  • the opening of the second door 53 is detected by the second opening / closing sensor S2.
  • the first door 47 of the culture chamber 15 is closed, and light from the outside does not enter the culture chamber 15.
  • Step S6 The CPU 61 determines whether or not the culture vessel 31 has been carried out from the outer standby section 35 of the light shielding box 51. This determination is made by an input from the presence / absence sensor S3 of the outer standby unit 35. The culture vessel 31 is carried out from the outer standby section 35 by the operator.
  • Step S7 The CPU 61 drives the motor M4 to close the second door 53 of the light shielding box 51.
  • the closing of the second door 53 is detected by the second opening / closing sensor S2.
  • an inner standby chamber 65 is formed inside the first door 47 of the culture chamber 15.
  • the inner standby chamber 65 causes the culture vessel 31 to temporarily wait.
  • the inner standby chamber 65 is formed by a bellows member 67 that can be expanded and contracted.
  • the bellows member 67 can be expanded and contracted toward the first door 47.
  • the bellows member 67 is urged in a direction opposite to the first door 47 by a spring (not shown), and is normally in an extended state.
  • the bellows member 67 is made of a light shielding material.
  • a light shielding member 69 is fixed to the transport robot 25.
  • the light shielding member 69 is fixed to an intermediate portion of the mini stage 25c.
  • the light shielding member 69 is disposed so as to surround the mini stage 25c and the rotary stage 25b. As shown in FIG. 7, in a state where the light blocking member 69 is in contact with the bellows member 67, the light incident from the bellows member 67 side is blocked by the light blocking member 69.
  • an outer standby part 35 for waiting the culture vessel 31 is arranged.
  • a presence / absence sensor S3 for detecting the presence / absence of the culture vessel 31 is disposed.
  • the culture container 31 is carried in and out as described below.
  • FIG. 9 is a flowchart showing the operation of bringing the culture vessel 31 into the culture chamber 15.
  • Step S1 The CPU 61 determines whether or not there has been an instruction to carry in the culture vessel 31.
  • the instruction to carry in the culture container 31 is performed by an operator operating the operation unit 41 to instruct the delivery of the culture container 31 and the position of the culture container 31 to be carried in the stocker 19.
  • Step S2 The CPU 61 determines whether or not the culture vessel 31 is placed in the outer standby unit 35. This determination is made by an input from the presence / absence sensor S3 of the outer standby unit 35. The culture vessel 31 is placed on the outer standby section 35 by the operator.
  • Step S3 The CPU 61 drives the transport robot 25 to bring the light shielding member 69 into contact with the bellows member 67 as shown in FIG.
  • Step S4 The CPU 61 drives the motor M3 to open the first door 47 of the culture chamber 15.
  • the opening of the first door 47 is detected by the first opening / closing sensor S1.
  • the light shielding member 69 is in contact with the bellows member 67, and light from the outside does not enter the culture chamber 15.
  • Step S5 The CPU 61 drives the transport robot 25 to carry the culture vessel 31 placed in the outer standby section 35 into the bellows member 67. More specifically, as shown in FIG. 8, the transport robot 25 is driven to pick up the culture vessel 31 placed in the outer standby unit 35. In this state, the bellows member 67 is contracted. And the culture container 31 is conveyed to the position in the bellows member 67 shown in FIG.
  • Step S6 The CPU 61 drives the motor M3 to close the first door 47 of the culture chamber 15. The closing of the first door 47 is detected by the first opening / closing sensor S1.
  • Step S7 The CPU 61 drives the transfer robot 25 to transfer the culture vessel 31 placed in the bellows member 67 to a predetermined position of the stocker 19.
  • FIG. 10 is a flowchart showing the operation of carrying out the culture vessel 31 from the culture chamber 15.
  • Step S1 The CPU 61 determines whether or not there is an instruction to carry out the culture vessel 31.
  • the instruction to carry out the culture vessel 31 is performed, for example, when the operator operates the operation unit 41 to instruct the position of the culture vessel 31 to be carried out and carried out in the stocker 19.
  • Step S2 The CPU 61 drives the transport robot 25 to carry the culture vessel 31 placed in the stocker 19 into the bellows member 67 and bring the light shielding member 69 into contact with the bellows member 67.
  • Step S3 The CPU 61 drives the motor M3 to open the first door 47 of the culture chamber 15.
  • the opening of the first door 47 is detected by the first opening / closing sensor S1.
  • the light shielding member 69 is in contact with the bellows member 67, and light from the outside does not enter the culture chamber 15.
  • Step S4 The CPU 61 drives the transfer robot 25 to carry out the culture vessel 31 placed in the bellows member 67 to the outer standby unit 35.
  • Step S5 The CPU 61 drives the motor M3 to close the first door 47 of the culture chamber 15. The closing of the first door 47 is detected by the first opening / closing sensor S1.
  • FIG. 11 shows a third embodiment of the culture apparatus of the present invention.
  • the same elements as those in the first embodiment are denoted by the same reference numerals, and detailed description thereof is omitted.
  • the light shielding box 51 of the first embodiment is not disposed, and only the outer standby unit 35 is disposed. And control by the control apparatus 55 is performed so that the 1st door 47 may not open at the time of observation by the observation part 23.
  • FIG. 1st door 47 may not open at the time of observation by the observation part 23.
  • FIG. 12 is a flowchart showing the operation of bringing the culture vessel 31 into the culture chamber 15.
  • Step S1 The CPU 61 determines whether or not there has been an instruction to carry in the culture vessel 31.
  • the instruction to carry in the culture container 31 is performed by an operator operating the operation unit 41 to instruct the delivery of the culture container 31 and the position of the culture container 31 to be carried in the stocker 19.
  • Step S2 The CPU 61 determines whether or not the culture vessel 31 is placed in the outer standby unit 35. This determination is made by an input from the presence / absence sensor S3 of the outer standby unit 35. The culture vessel 31 is placed on the outer standby section 35 by the operator.
  • Step S3 The CPU 61 obtains the time during which the external light is allowed to enter the culture chamber 15 from the observation schedule stored in the memory 63.
  • the observation time for each culture vessel 31 is stored as a schedule table in relation to the management number (No. 1 etc.) of the culture vessel 31.
  • a blank K in the schedule table indicates a state where there is no culture vessel 31 to be observed.
  • the CPU 61 determines the blank time closest to the current time as the permitted time.
  • Step S4 The CPU 61 determines whether or not the permitted time has come.
  • Step S5 The CPU 61 opens the first door 47 of the culture chamber 15 when the permitted time comes.
  • Step S6 The CPU 61 drives the transfer robot 25 to transfer the culture vessel 31 placed in the outer standby unit 35 to the stocker 19.
  • Step S7 The CPU 61 drives the motor M3 to close the first door 47 of the culture chamber 15.
  • FIG. 14 is a flowchart showing the operation of unloading the culture vessel 31 from the culture chamber 15.
  • Step S1 The CPU 61 determines whether or not there is an instruction to carry out the culture vessel 31.
  • the instruction to carry out the culture vessel 31 is performed, for example, when the operator operates the operation unit 41 to instruct the position of the culture vessel 31 to be carried out and carried out in the stocker 19.
  • Step S2 The CPU 61 obtains the time when the external light is allowed to enter the culture chamber 15 from the observation schedule stored in the memory 63. Since this is the same as step S3 in FIG.
  • Step S3 The CPU 61 determines whether or not the permitted time has come.
  • Step S4 The CPU 61 opens the first door 47 of the culture chamber 15 when the permitted time comes.
  • Step S5 The CPU 61 drives the transfer robot 25 to transfer the culture vessel 31 placed on the stocker 19 to the outer standby unit 35.
  • Step S6 The CPU 61 drives the motor M3 to close the first door 47 of the culture chamber 15.
  • FIG.15 and FIG.16 has shown 4th Embodiment of the culture apparatus of this invention.
  • the same elements as those in the first embodiment are denoted by the same reference numerals, and detailed description thereof is omitted.
  • the inside of the culture chamber 15 is provided.
  • a light shielding box 71 is disposed on the screen.
  • the illumination unit 21 and the observation unit 23 are arranged inside the light shielding box 71.
  • the illumination unit 21 and the observation unit 23 are included in the light shielding box 71.
  • the same effect can be obtained even if the light shielding box 71 is configured from a light shielding cover that covers the upper surfaces and side surfaces of the illumination unit 21 and the observation unit 23.
  • the area to be observed inside the culture vessel 31 is several tens to several hundreds mm ⁇ several tens to several hundreds mm.
  • 400 images are acquired by the CCD camera 45 installed in the microscope 33. That is, in observing the sample inside the culture vessel 31, the culture vessel 31 is placed on the observation unit 23 for several tens of minutes to several hours.
  • the light shielding box 71 including the illumination unit 21 and the observation unit 23 a light shielding box having the following configuration is used.
  • each wall of the light shielding box 71 has a shape in which, for example, the end edges of the plurality of thin plates 72a to 72d overlap each other and are spaced apart from each other. That is, each wall of the light shielding box 71 is formed by alternately arranging a plurality of plates at a constant interval, so that the cell culture environment inside the light shielding box 71 and the cell culture environment outside the light shielding box 71 are formed. While being the same, the structure prevents light from entering the inside of the light shielding box 71. It should be noted that the number of laminated thin plates is not limited to four, and the light shielding performance inside the light shielding box 71 and the cell culture environment inside and outside the light shielding box 71 may be kept the same. If possible, it may be set appropriately.
  • the light shielding box 71 is provided with an open / close door 73 for taking the culture vessel 31 in and out.
  • the open / close door 73 is provided on the side surface of the light shielding box 71 that faces the transfer robot 25.
  • the opening / closing door 73 is opened and closed by moving up and down by a door opening / closing motor M5.
  • the open / close state of the open / close door 73 of the light shielding box 71 is recognized by the sensor 74.
  • the motor driven when the doors are opened and closed is controlled so that the door 73 and the first door 47 are not opened and closed at the same time.
  • the culture container 31 is carried in and out as described below.
  • the culture container 31 is installed in an external standby place (the above-described external standby section 35).
  • the first door 47 is opened by an instruction from the operation unit 41.
  • the open / close door 73 of the light shielding box 71 is closed.
  • the transfer robot 25 is driven to carry the culture vessel 31 into the culture chamber 15.
  • the culture container 31 is carried into the culture chamber 15 by the transfer robot 25, the first door 47 is closed.
  • the open / close door 73 of the light shielding box 71 is opened. At this time, the first door 47 is closed. The opening of the open / close door 73 is detected by the sensor 74.
  • the transport robot 25 transports the culture vessel 31 to the observation unit 23 disposed inside the light shielding box 71. After transporting the culture vessel 31 to the observation unit 23, the transport robot 25 moves to the outside of the light shielding box 71. After the operation of the transfer robot 25, the open / close door 73 of the light shielding box 71 is closed. In this state, the observation unit 23 is shielded by the light shielding box 71, and external light that enters the inside of the culture chamber 15 when the first door 47 is opened is shielded by the light shielding box 71. The Further, since the light shielding box 71 has the above-described structure, the cell culture environment inside the light shielding box 71 can be maintained in the same cell culture environment as the culture chamber 15.

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Abstract

La présente invention porte sur un appareil de culture caractérisé en ce qu'il comprend : une chambre de culture dans laquelle est formée une chambre noire et contenant un récipient de culture , la chambre noire étant maintenue dans une atmosphère prédéfinie ; un moyen d'observation pour l'observation d'un échantillon placé dans le récipient de culture qui est contenu dans la chambre de culture ; une première porte qui est élaborée dans la chambre de culture et à travers laquelle le récipient de culture doit être transporté dans la chambre de culture à partir de l'extérieur ou transporté hors de la chambre de culture ; et un moyen faisant écran à la lumière pour empêcher l'entrée de lumière provenant de l'extérieur dans le moyen d'observation lors de l'ouverture de la première porte.
PCT/JP2009/007267 2009-01-05 2009-12-25 Appareil de culture WO2010076849A1 (fr)

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JP2009000336 2009-01-05
JP2009-000336 2009-01-05

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105527450A (zh) * 2015-12-31 2016-04-27 中国科学院苏州生物医学工程技术研究所 一种用于全自动检测仪器的微孔板传送装置
WO2016170623A1 (fr) * 2015-04-22 2016-10-27 株式会社安川電機 Système de culture cellulaire et procédé de culture cellulaire

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Publication number Priority date Publication date Assignee Title
JP2007228863A (ja) * 2006-02-28 2007-09-13 Olympus Corp 閉鎖型反応検出装置
WO2007105363A1 (fr) * 2006-03-14 2007-09-20 Nikon Corporation Equipement d'observation de cultures
JP2007330143A (ja) * 2006-06-14 2007-12-27 Nikon Corp 培養容器規制体，ホルダ及び培養装置
JP2008268019A (ja) * 2007-04-20 2008-11-06 Hitachi Ltd 化学発光計測装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007228863A (ja) * 2006-02-28 2007-09-13 Olympus Corp 閉鎖型反応検出装置
WO2007105363A1 (fr) * 2006-03-14 2007-09-20 Nikon Corporation Equipement d'observation de cultures
JP2007330143A (ja) * 2006-06-14 2007-12-27 Nikon Corp 培養容器規制体，ホルダ及び培養装置
JP2008268019A (ja) * 2007-04-20 2008-11-06 Hitachi Ltd 化学発光計測装置

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016170623A1 (fr) * 2015-04-22 2016-10-27 株式会社安川電機 Système de culture cellulaire et procédé de culture cellulaire
JPWO2016170623A1 (ja) * 2015-04-22 2017-10-12 株式会社安川電機 細胞培養システム及び細胞培養方法
CN105527450A (zh) * 2015-12-31 2016-04-27 中国科学院苏州生物医学工程技术研究所 一种用于全自动检测仪器的微孔板传送装置

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