WO2010052791A1 - 植物またはキノコからの有効成分の高効率抽出法 - Google Patents
植物またはキノコからの有効成分の高効率抽出法 Download PDFInfo
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- WO2010052791A1 WO2010052791A1 PCT/JP2008/070328 JP2008070328W WO2010052791A1 WO 2010052791 A1 WO2010052791 A1 WO 2010052791A1 JP 2008070328 W JP2008070328 W JP 2008070328W WO 2010052791 A1 WO2010052791 A1 WO 2010052791A1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0215—Solid material in other stationary receptacles
- B01D11/0223—Moving bed of solid material
- B01D11/0226—Moving bed of solid material with the general transport direction of the solids parallel to the rotation axis of the conveyor, e.g. worm
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/05—Stirrers
- B01F27/11—Stirrers characterised by the configuration of the stirrers
- B01F27/19—Stirrers with two or more mixing elements mounted in sequence on the same axis
- B01F27/192—Stirrers with two or more mixing elements mounted in sequence on the same axis with dissimilar elements
- B01F27/1921—Stirrers with two or more mixing elements mounted in sequence on the same axis with dissimilar elements comprising helical elements and paddles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/21—Mixers with rotary stirring devices in fixed receptacles; Kneaders characterised by their rotating shafts
- B01F27/2123—Shafts with both stirring means and feeding or discharging means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/60—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis
Definitions
- the present invention is a method for extracting active ingredients from plants or mushrooms with high efficiency and low energy consumption.
- This circulation multi-stage pressure extraction method has the disadvantages that it takes many processing steps and takes a lot of time and energy consumption.
- a method has been proposed in which extraction is performed after heat treatment with oil at 100 to 300 ° C. for the purpose of breaking the cell membrane and increasing the extraction efficiency (see Patent Document 2).
- This pretreatment with high-temperature oil has the disadvantage that the lipophilic active ingredient is dissolved in the oil from cell membranes and cell fluids destroyed by high heat.
- the present invention provides a method for extracting an active ingredient from plants or mushrooms with high yield and energy saving.
- the present invention relates to a method for extracting an active ingredient from a plant or mushroom, in a container closed from the viewpoint of pressure in a mixture comprising 1 part by weight of the substance and 2 to 7 parts by weight of water, preferably 2 to 5 parts by weight.
- Aqueous phase containing active ingredient and crushed solid material by crushing the material to a mean value of maximum dimension of 1-20 ⁇ m by stirring under conditions giving high shear force at a temperature of 100-250 ° C. It is a method characterized in that it comprises a step of extracting active ingredients by obtaining a crushed mixture consisting of residues and then recovering the aqueous phase.
- crushing is preferably performed at 100 to 130 ° C.
- the first filtration residue is 20 to 100 ° C higher than the first crushing temperature (a temperature not exceeding 250 ° C).
- the total amount of active ingredients extracted is preferably at least 30% by weight, more preferably at least 45% by weight.
- crushing is preferably performed at 100 to 130 ° C. in as short a time as possible.
- the additional steps 1 to 3 are preferably performed at a temperature that is 20 to 100 ° C. higher than the temperature in the first step and does not exceed 250 ° C.
- the total extraction amount of active ingredients is preferably achieved at least 30% by weight, more preferably at least 45% by weight.
- an inert gas such as nitrogen gas
- the present inventor attempted to destroy the cell membrane by pulverizing a plant or mushroom with a high temperature, high pressure, high shear force kneader. Then, it was found that the cell membrane was broken to 1 to 20 ⁇ m by this pulverization, and the intracellular fluid easily came out of the cell. The present inventor further reduced the raw material plant or mushroom (hereinafter sometimes referred to as the substance) to an average value of a maximum size of 5 to 20 mm in advance, and increased the high temperature, high pressure, and high shear force. By pulverizing with a kneader (see FIG.
- the material is preferably reduced to 100 to 500 ⁇ m, and further treated with a kneader under a pressure of 0.5 to 5 MPa depending on the hardness of the material, the material is reduced to 1 to 20 ⁇ m.
- the thermocouple that measures the temperature of the object to be processed can be further reduced, and an abnormal current corresponding to a temperature that is at least 50 ° C., in particular at least 100 ° C., more particularly at least 200 ° C. higher or lower than the processing temperature. (See FIG. 3).
- the transition to the aqueous phase is due to osmotic pressure, so that the amount transferred is only about 8% by weight of the dry raw material.
- the obtained aqueous phase was analyzed, it was found that at least 15% by weight of the dry raw material was extracted into the aqueous phase. Since the maximum amount extracted by the decoction method is 8%, the original water-soluble substance is considered to be 10% by weight of the dry raw material. The further extraction amount in the present invention is presumed that the cell constituents were extracted by the destruction of the cell membrane by shearing.
- the above-mentioned abnormal current picked up by the thermocouple is caused by radicals generated by the polymer compound that composes the cell membrane being cut by shearing force, and electrons from this radical are converted into abnormal currents. Presumed to have been captured. It is considered that physical disruption of chemical bonds of cell membrane constituents occurred by crushing to 1 to 20 ⁇ m, and the resulting hydrolysis caused an efficient hydrolysis reaction of the cell membrane constituent polymer substances. As a result, the cell membrane is destroyed, the cell membrane constituent polymer substance is hydrolyzed and the active ingredient is easily extracted, and the intracellular fluid comes out to the hot water side, so that the extraction of the active ingredient is less than the conventional method. It became easy to do with energy consumption.
- the generated radicals further cause the cleavage of the high molecular compounds that make up the cell membrane, and the repetition of this is effective for at least 15% by weight of the dry raw materials, including those that break down the intracellular active ingredients and cell constituents into small molecules. It is presumed that the component has been extracted.
- the solid substance residue remaining after recovering the aqueous phase preferably has a water content of 40-60%.
- the total amount of extracted active ingredients is preferably at least 30% by weight. More preferably at least 45% by weight.
- the number of additional repetitions depends on the hardness of the cell membrane of the substance, and is preferably 1 to 2 times for soft materials such as mushrooms and 2 to 3 times for hard plants. Even if the processing is continued four times or more, it is not easy to increase the recovery of the active ingredient commensurate with the processing cost.
- FIG. 1 shows an external appearance of a stirring device by cutting a cylindrical container 20 at the center in the axial direction.
- a screw 11 that feeds the substance from the substance input port 4 to a rotating shaft 5 connected to a motor 9, a forward blade 12 that advances an unprocessed object in the axial direction, and a flow of an object to be processed sent forward
- a swept wing 13 is connected that reverses the direction and returns it back in a region close to the axis.
- Each forward blade 12 is composed of four blades attached at equal intervals in the circumferential direction of the shaft, and the direction of the blades is angled so as to advance the workpiece when the shaft is rotated.
- the forward advancing blade 12 ′ is supported by four elongate attachment plates 14 attached at equal intervals in the circumferential direction of the shaft, and a workpiece can flow between the four elongate attachment plates 14.
- the retreating wing 13 causes the object to be processed to flow backward when the shaft is rotated.
- the pressure in the container exceeds a predetermined value due to water vapor and other gas generated during processing, a part of the water vapor and other gas is discharged from the discharge pipe 1.
- the pressure near the inlet is monitored by a Bourdon tube pressure gauge.
- the inlet temperature is monitored by thermocouple 3.
- the temperature and pressure in the processing area of the vessel is monitored by a thermocouple and pressure transmitter 6 and a sanitary oil-free pressure sensor ASG 702 and a thermocouple 7.
- an inert gas pipe 8 is provided to introduce a high-pressure inert gas for increasing the pressure in the container.
- a jacket for heating the container is further provided (not shown).
- the feed screw 11 is advanced, and a predetermined amount of the substance is introduced. Close the substance inlet. The product outlet 10 is closed.
- the plant or mushroom containing the active ingredient will generally have a size of several tens to several hundreds of millimeters at first, but it is preferable that the average value of the maximum dimension of 5 to 20 mm is previously finely divided. . It is preferable to make this fine in advance so as to have an average value of the maximum dimension of 100 to 500 ⁇ m in the above apparatus.
- the temperature of the workpiece is preferably set to 0 to 50 ° C., more preferably 5 to 30 ° C., and the pressure is atmospheric pressure. This process is hereinafter referred to as a pre-grinding process. Next, the process according to the present invention will be described.
- the material which has been finely divided in advance to an average value of a maximum size of 5 to 20 mm without adding water has an average value of a maximum size of 100 to 500 ⁇ m by a high shearing force. Make it fine.
- Water is added to the pre-ground plant or mushroom (the material).
- the weight ratio of the substance to water is set to 1 part by weight of the substance and 2 to 7 parts by weight of water, preferably 1 part by weight of the substance and 3 to 5 parts by weight of water.
- the container is then sealed and heated to bring the temperature of the contents to 100-250 ° C, preferably 110-240 ° C, more preferably 120-230 ° C.
- the substance can be crushed to an average value of the maximum dimension of 1 to 20 ⁇ m, and at least 15% by weight of the polymer substance and the intracellular substance constituting the cell membrane in the substance can be extracted. It is important to apply sufficient shear to the material. Such high shear force does not occur when the above-described apparatus is operated without any special measures.
- the ratio of the return amount of the mixture in the backward direction with the blade is set to 1: 0.6 to 0.9, preferably 1: 0.65 to 0.85, more preferably 1: 0.7 to 0.8.
- an active ingredient can be obtained in large quantities (with a high extraction rate) from the plant or mushroom containing an active ingredient, Therefore, an active ingredient can be manufactured simply and cheaply.
- Examples of the plant or mushroom containing the active ingredient in the present invention include ganoderma, mesimacob, dokudami, glaze, licorice root and the like.
- the substance containing the active ingredient having an average value of the largest dimension of 5 to 20 mm is pre-ground to 100 to 500 ⁇ m and then to a temperature of 100 to 250 ° C. to 1 to 20 ⁇ m. Crushing takes place.
- An example of a small prototype of an apparatus suitable for carrying out the present invention described above is shown in FIG. 1 and is a closed type stirring apparatus having a motor of 20 liters and 5.5 kW. Pre-pulverization is performed at normal temperature and normal pressure. The substance containing the active ingredient is ground to 100-500 ⁇ m.
- the powder is crushed to 1 to 20 ⁇ m. Is done.
- the pressure in the reaction vessel is increased to 0.5 to 5 MPa by an inert gas.
- the pressure at this time is changed depending on the hardness of the cell membrane of the substance. Soft ones are under low pressure and hard ones are under high pressure. This pressurization increases the shearing force, and the substance containing the active ingredient is easily pulverized to 1 to 20 ⁇ m.
- the stirring device may be either a batch type or a continuous type which is closed in terms of pressure.
- the continuous stirrer includes the introduction of the substance containing the active ingredient, the extraction of the extract containing the extraction residue, and the injection and extraction of an inert gas such as carbon dioxide and nitrogen used for pressurization. What is necessary is just to be able to carry out continuously, maintaining a predetermined condition.
- the upper limit of the heating temperature is 250 ° C, preferably 240 ° C, more preferably 230 ° C, and the lower limit is 100 ° C, preferably 110 ° C, more preferably 120 ° C.
- the upper limit of the heating time is preferably 3 hours, more preferably 2 hours, still more preferably 1.5 hours, particularly preferably 1 hour, and the lower limit is preferably 5 minutes, more preferably 15 minutes, still more preferably 20 minutes. is there.
- the cell membrane constituent polymer material is destroyed by hydrolysis by radicals generated by the physical chemical bond cleavage generated by grinding the substance containing the active ingredient to 1 to 20 ⁇ m, and the intracellular substance exits out of the cell. In this way, the active ingredient is converted into an extractable substance.
- the lower limit of the pressure during crushing is 0.5 to 1 MPa, preferably 1.0 to 1 MPa, and more preferably 1.5 to 1 MPa.
- the upper limit is 5.0 MPa, preferably 4.5 MPa, more preferably 4.0 MPa, from the production cost of the apparatus.
- the pressurization to the predetermined pressure is preferably performed using an inert gas, for example, nitrogen gas, and the pressure applied to the sample is adjusted within the above range.
- the shear force is further increased by pressurization as described above.
- the upper limit of the shear force at the time of crushing (and pre-pulverization) according to the present invention is 20 MPa, preferably 10 MPa, more preferably 5 MPa, still more preferably 3 MPa, and the lower limit is 0.1 MPa, preferably 0.3 MPa, more preferably 0.5 MPa. MPa. If the upper limit is exceeded, the motor power load increases and the processing cost increases, and if it is less than the lower limit, the preliminary pulverization is insufficient and the substance is not sufficiently decomposed during pulverization according to the present invention.
- the shearing force is given by a stirring blade provided in the stirring device.
- the shear force was measured by the method described in PCT / JP2004 / 013551.
- Reference materials with known viscosities (20 ° C) for example, standard solutions for viscosity calibration (JIS Z8809) JS100 viscosity 86 mPa ⁇ s, JS14000 viscosity 12 Pa ⁇ s, and JS10000 viscosity 140 Pa ⁇ s manufactured by Nippon Grease Co., Ltd.
- the torque applied to the rotating shaft is measured by rotating the stirring blade provided at 20 rpm at a temperature of 20 ° C.
- a mixture prepared by mixing kerosene into asphalt for example, viscosity measured using a BS type viscometer manufactured by Toki Sangyo Co., Ltd. (20 ° C)
- the torque is measured in the same manner as described above using a mixed solution of 6400 Pa ⁇ s.
- the measurement liquid is added until the entire stirring blade in the stirring device is completely in the liquid. Further, the torque in an empty state in which the measurement liquid is not put into the stirring device is measured (the shearing force at this time is set to zero).
- the shear rate is represented by the following formula.
- sin 3.0 ° is a value unique to the apparatus shown in FIG. The value is determined by the shape of the stirring blade, and varies depending on the shape of the stirring blade.
- Shear rate (s-1) Shear rate (s-1) ⁇ 2 ⁇ 3.14 ⁇ (Number of rotations per second) ⁇ sin3.0 °
- the shear force can be obtained by measuring the torque applied to the rotating shaft. Since the shaft torque of the stirring device provided with the stirring blades is unique to the device, the torque changes as the device changes. Therefore, the relationship between the torque and the shearing force as shown in FIG. Thus, in any apparatus, the shear force can be obtained by measuring the torque applied to the rotating shaft.
- the flow from the inlet direction and the flow from the outlet direction collide with each other to create a flow toward the outer wall of the stirring device, and the strength of this flow can be detected as pressure at a position of 7.
- the shearing force obtained from FIG. 2 was found to be the same as the value measured using the sanitary type oil-free pressure sensor ASG702 from Yamatake Corporation. .
- Example 1 EXAMPLES Hereinafter, although an Example demonstrates this invention still in detail, this invention is not limited by an Example.
- Ganoderma used in Example 1 has the properties shown in Table 1 below. (table 1) Ganoderma size 40-50mm in length, 5-10mm in thickness Moisture 11.5% by weight
- the moisture in Table 1 above was measured using an infrared moisture meter FD-720 manufactured by Kett Science Laboratory.
- the stirring device shown in FIG. 1 was used as the stirring blade.
- As the stirring blade a calculated value of the return amount from the opposite side to the calculated value of the feed amount of the screw feeder at the sample insertion port 4 with a return of 0.8 with respect to the feed of 1.0 was used.
- the capacity was 20 liters and it was equipped with a 5.5 kW motor.
- the instruction of the pressure sensor ASG702 (7 in FIG.
- Example 2 Component analysis was performed on the freeze-dried products of the aqueous phase of Example 1 and Reference Example, and the results are shown in Table 2.
- Table 2 the extraction conditions and extraction rate of the marketed product of Ganoderma lucidum extract and the component analysis value of the extract are shown, and the results are also shown in Table 2.
- the extraction rate of the reference example is 27.3%, but the extraction rate of Example 1 is 40.3%, 38.6%. Furthermore, the extraction amount of ⁇ -glucan, which is said to be useful for maintaining health, is reduced by 50% in Example 1, and the extraction amount of water-soluble dietary fiber that is useful for adjusting the intestinal environment is also increased by 2.25 times.
- the amount of water used for extraction is 30 kg, which is 7.5 times the amount of the conventional method compared to 4 kg of the present invention per kg of ganoderma. It is.
- the conventional method 7.5 times the amount of water is heated to near 100 ° C. and held for 30 minutes, and concentrated by freeze distillation and freeze-dried to produce a powdered ganoderma extract.
- the present method has the advantage that it can be directly freeze-dried without the need for a concentration step by vacuum distillation because of the small amount of water, which is 1 / 7.5.
- the extraction amount of ⁇ -glucan which is said to be useful for maintaining health, is increased by 2.2 times, and the extraction amount of water-soluble dietary fiber is That's up 46.8 times.
- about 18% of the extracted saccharides are ⁇ -glucan, compared with about 8% in the conventional method (Table 2).
- the method according to the present invention not only achieves significant energy saving compared with the conventional extraction method, but also greatly increases the amount of extraction of the active ingredient.
- the present invention can extract and produce active ingredients from plants or mushrooms with high efficiency and low energy consumption.
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Abstract
Description
有効成分として熱分解を起こしやすいテルペン類等を含む場合には、破砕は100~130℃でできる限り短時間で行うことが好ましく、短時間での抽出の効率を上げるためには、窒素ガス等の不活性ガスで0.5~5MPaに加圧し、加圧による剪断力の増加を図ることができるところの方法である。また全体としての有効成分の抽出効率を高めるために、第1回目の濾過残渣に対して、第1回の破砕温度よりも20~100℃高い温度(250℃を超えない範囲の温度)での破砕を2~3回繰り返すことによって、合計の有効成分の抽出量は好ましくは少なくとも30重量%、より好ましくは少なくとも45重量%が抽出される。
本発明者は更に、原料である植物またはキノコ(以下で、該物質と言うことがある)が予めが5~20mmの最大寸法の平均値へと小さくし、これを高温・高圧・高剪断力ニーダー(図1参照)により粉砕することによって好ましくは100~500μmへと小さくし、さらに該物質の硬さの違いによって0.5~5 MPa加圧下でニーダーで処理すると、該物質を1~20μmへと更に小さく出来、その際に被処理物の温度を測定する熱電対が、該処理温度に比べて少なくとも50℃、特には少なくとも100℃、より特には少なくとも200℃高いまたは低い温度に相当する異常電流を捕捉することを発見した(図3参照)。従来の煎じ方法では水性相への移行は浸透圧によるため、移行する量は乾燥原料の約8重量%に過ぎない。本発明においては、得られた水性相を分析すると、乾燥原料の少なくとも15重量%が水性相に抽出されたことが分った。煎じ法で抽出される量は最大8%であるので、元来の水溶性物質は乾燥原料の粗10重量%であると考えられる。本発明におけるこれ以上の抽出量は、剪断による細胞膜の破壊によって細胞構成物質が抽出されたものと推定される。
次に、本発明に従う工程について説明を行う。先ず予備粉砕工程においては、水を加えない状態で予め5~20mmの最大寸法の平均値の大きさに細かくしておいた該物質を高剪断力によって100~500μmの最大寸法の平均値を有するように細かくする。この予備粉砕された植物またはキノコ(該物質)に水を加える。該物質と水との重量比は、該物質1重量部と水2~7重量部、好ましくは該物質1重量部と水3~5重量部に設定される。
また、該攪拌装置はバッチ式、又は圧力の点では閉じている連続式のいずれであってもよい。連続式の攪拌装置は、有効成分を含む該物質の装入及び抽出残渣を含んだ抽出液の抜き出し、並びに加圧に使用した炭酸ガスや窒素などの不活性ガスの圧入及び抜き出しを本発明の所定の条件を維持しつつ連続的に実施し得るものであればよい。
(数1)
剪断力(Pa)=[粘度(Pa・s)×剪断速度(s-1)]/トルクの読み取り値
から剪断力を求めて、例えば図2に示すトルクと剪断力との関係を得る。上記の式中、剪断速度は下記式で表される。下記式においてsin3.0°は、図1に示す装置固有の値である。該値は攪拌羽根の形状により求められ、攪拌羽根の形状により相違する。
(数2)
剪断速度(s-1)≒2×3.14×(1秒当りの回転数)÷sin3.0°
以下、本発明を実施例により更に詳細に説明するが、本発明は実施例により限定されるものではない。
(表1)
霊芝
大きさ 縦横40~50mm、厚さ5~10mm
水分 11.5重量%
2.ブルドン管圧力計
3.熱電対
4.試料投入口
5.剪断力を与えることが出来る撹拌羽根
6.熱電対と圧力伝送器
7.サニタリー型オイルフリー圧力センサーASG702と熱電対
8.不活性ガスによる加圧ライン
9.モーター
10.試料排出口
11,送り翼
12.前進翼
13.後退翼
14.取り付け板
20.円筒形の容器
Claims (13)
- 植物またはキノコからの有効成分の抽出方法において、該物質1重量部と水2~7重量部とから成る混合物を、圧力の観点で閉じた容器中で100~250℃の温度で高剪断力を与える条件下で攪拌して該物質を1~20μmの最大寸法の平均値へと破砕することによって、有効成分を含む水相および破砕された固形物質残渣からなる破砕混合物を得、次に該水相を回収することによって、有効成分を抽出する工程を含むことを特徴とする方法。
- 水相を回収したのちに残る固形物質残渣を、請求項1記載の工程に更に1~3回付すこと、ただし該1~3の追加の工程は、第1回の工程における温度よりも20~100℃高い温度でありかつ250℃を超えない温度で行われる、請求項1記載の方法。
- 該容器が円筒形であり、その中心軸方向に延びる回転軸、該回転軸に備えられた前進翼及び後退翼を有し、該前進翼により前進方向へと上記混合物を運ぶ送り量の計算値と、該後退翼により後退方向へと上記物質を運ぶ戻り量の計算値との比が1:0.6~0.9である請求項1及び2に記載の方法。
- 上記の比が1:0.65~0.85である請求項3記載の方法。
- 上記の比が1:0.7~0.8である請求項3記載の方法。
- 攪拌により該物質に0.1~20 MPaの剪断力が懸かる請求項1~5のいずれか1項に記載の方法。
- 剪断力が0.3~10 MPaである請求項6に記載の方法。
- 攪拌の際に該混合物の温度を測定する熱電対が、該混合物の温度に比べて少なくとも50℃高いまたは低い温度に相当する異常電流を捕捉する請求項1~7のいずれか1項に記載の方法。
- 攪拌の際に該混合物の温度を測定する熱電対が、該混合物の温度に比べて少なくとも100℃高いまたは低い温度に相当する異常電流を捕捉する請求項1~8のいずれか1項に記載の方法。
- 植物またはキノコが5~20mmの最大寸法の平均値を有する請求項1~9のいずれか1項に記載の方法。
- 該容器内の圧力を、不活性気体を用いて0.5~5.0 MPaに加圧する、請求項1~10のいずれか1項に記載の方法。
- 破砕抽出工程が5分間~3時間行われる、請求項1~11のいずれか1項に記載の方法。
- 植物またはキノコを1~20μmの最大寸法の平均値へと破砕する過程で生じるすり潰し効果によって、細胞膜が破壊され、細胞内に含まれている有効成分が水性層に抽出されることを特徴とする請求項1~12のいずれか1項に記載の方法。
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CN200880131890.2A CN102215929B (zh) | 2008-11-07 | 2008-11-07 | 从植物或蘑菇高效率地提取有效成分的方法 |
KR1020117011559A KR101313390B1 (ko) | 2008-11-07 | 2008-11-07 | 식물 또는 버섯의 유효 성분의 고효율 추출 방법 |
PCT/JP2008/070328 WO2010052791A1 (ja) | 2008-11-07 | 2008-11-07 | 植物またはキノコからの有効成分の高効率抽出法 |
JP2010536625A JP5590613B2 (ja) | 2008-11-07 | 2008-11-07 | 植物またはキノコからの有効成分の高効率抽出法 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012035226A (ja) * | 2010-08-10 | 2012-02-23 | Kem:Kk | 粉砕装置 |
CN113866205A (zh) * | 2021-12-06 | 2021-12-31 | 天津海关动植物与食品检测中心 | 一种中草药荧光光谱重金属检测装置和检测方法 |
CN115382244A (zh) * | 2022-08-30 | 2022-11-25 | 湖州安然生物医药科技有限公司 | 一种化妆品用复合海藻提取物的制备装置及制备方法 |
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CN113101689B (zh) * | 2021-03-17 | 2022-12-06 | 贵州省工程复合材料中心有限公司 | 一种香菇多糖提取浓度的控制方法及其提取装置 |
Citations (5)
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WO1996041855A1 (fr) * | 1995-06-13 | 1996-12-27 | Nippon Petrochemicals Company, Limited | Procede et dispositif d'extraction continue de cire brute |
JP2002030101A (ja) * | 2000-07-17 | 2002-01-31 | Cremona:Kk | アガリクスブラゼイ(AgaricusBlazeiMurill)に含まれるβ−D−グルカンを液中に抽出する製法 |
JP2006334489A (ja) * | 2005-06-01 | 2006-12-14 | Sasaki Corporation | 生物系物質の破砕成分抽出装置 |
JP2007301472A (ja) * | 2006-05-11 | 2007-11-22 | Oji Paper Co Ltd | バイオマス連続的加圧熱水処理方法 |
JP2007313442A (ja) * | 2006-05-26 | 2007-12-06 | Tokyo Electric Power Co Inc:The | マイクロ波抽出法及び抽出装置 |
Family Cites Families (1)
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CN1161056A (zh) * | 1995-06-13 | 1997-10-01 | 日本石油化学株式会社 | 粗蜡的连续萃取方法及其所用设备 |
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2008
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- 2008-11-07 WO PCT/JP2008/070328 patent/WO2010052791A1/ja active Application Filing
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1996041855A1 (fr) * | 1995-06-13 | 1996-12-27 | Nippon Petrochemicals Company, Limited | Procede et dispositif d'extraction continue de cire brute |
JP2002030101A (ja) * | 2000-07-17 | 2002-01-31 | Cremona:Kk | アガリクスブラゼイ(AgaricusBlazeiMurill)に含まれるβ−D−グルカンを液中に抽出する製法 |
JP2006334489A (ja) * | 2005-06-01 | 2006-12-14 | Sasaki Corporation | 生物系物質の破砕成分抽出装置 |
JP2007301472A (ja) * | 2006-05-11 | 2007-11-22 | Oji Paper Co Ltd | バイオマス連続的加圧熱水処理方法 |
JP2007313442A (ja) * | 2006-05-26 | 2007-12-06 | Tokyo Electric Power Co Inc:The | マイクロ波抽出法及び抽出装置 |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012035226A (ja) * | 2010-08-10 | 2012-02-23 | Kem:Kk | 粉砕装置 |
CN113866205A (zh) * | 2021-12-06 | 2021-12-31 | 天津海关动植物与食品检测中心 | 一种中草药荧光光谱重金属检测装置和检测方法 |
CN115382244A (zh) * | 2022-08-30 | 2022-11-25 | 湖州安然生物医药科技有限公司 | 一种化妆品用复合海藻提取物的制备装置及制备方法 |
CN115382244B (zh) * | 2022-08-30 | 2023-06-16 | 湖州安然生物医药科技有限公司 | 一种化妆品用复合海藻提取物的制备装置及制备方法 |
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JP5590613B2 (ja) | 2014-09-17 |
CN102215929B (zh) | 2017-03-22 |
CN102215929A (zh) | 2011-10-12 |
KR20110082178A (ko) | 2011-07-18 |
KR101313390B1 (ko) | 2013-10-01 |
JPWO2010052791A1 (ja) | 2012-03-29 |
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