WO2010013906A3 - 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및 상기 방법에 의해 분리된 고활성 줄기 세포 - Google Patents

인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및 상기 방법에 의해 분리된 고활성 줄기 세포 Download PDF

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WO2010013906A3
WO2010013906A3 PCT/KR2009/003954 KR2009003954W WO2010013906A3 WO 2010013906 A3 WO2010013906 A3 WO 2010013906A3 KR 2009003954 W KR2009003954 W KR 2009003954W WO 2010013906 A3 WO2010013906 A3 WO 2010013906A3
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stem cells
highly active
separating
separated
human
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PCT/KR2009/003954
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English (en)
French (fr)
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WO2010013906A2 (ko
WO2010013906A9 (ko
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김효수
이은주
강현재
이하늘
김금현
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서울대학교병원
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Priority to EP09803111A priority Critical patent/EP2322602A4/en
Priority to US13/056,279 priority patent/US20110142809A1/en
Priority to MX2011000993A priority patent/MX2011000993A/es
Priority to CN2009801297934A priority patent/CN102112599A/zh
Priority to JP2011521015A priority patent/JP2011529340A/ja
Priority to CA2732025A priority patent/CA2732025A1/en
Publication of WO2010013906A2 publication Critical patent/WO2010013906A2/ko
Publication of WO2010013906A3 publication Critical patent/WO2010013906A3/ko
Publication of WO2010013906A9 publication Critical patent/WO2010013906A9/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

본 발명은 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법, 상기 방법에 의해 분리된 고활성 줄기세포, 상기 줄기세포를 포함하는 세포 치료제 및 특정 사이토카인을 포함하는 줄기세포에서 고활성 줄기세포를 분리하기 위한 배지에 관한 것이다. 본 발명에 따르면, 본 발명의 방법을 이용시 다양한 기원의 중배엽 줄기세포에서 고효율의 줄기세포를 분리해 내는데 매우 유용할 것이며, 또한 다른 조건에서 배양되고 있는 다양한 기원의 줄기세포에서도 적용이 가능하므로, 고효율성의 세포 치료제를 개발하는데 매우 유용하게 이용될 수 있을 것이다. 체외에서 여러 계대에 따른 증가되는 노화된 줄기세포를 효과적으로 분리해 낼 수 있으며, 이에 따라 줄기세포의 활성을 되돌릴 수 있는 방법으로 제시될 수 있다.
PCT/KR2009/003954 2008-07-28 2009-07-17 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및 상기 방법에 의해 분리된 고활성 줄기 세포 WO2010013906A2 (ko)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP09803111A EP2322602A4 (en) 2008-07-28 2009-07-17 METHOD FOR SEPARATING HIGH-ACTIVE STEM CELLS OF HUMAN STEM CELLS AND HIGH-ACTIVE STEM CELLS THUS SEPARATED IN THIS WAY
US13/056,279 US20110142809A1 (en) 2008-07-28 2009-07-17 Method for separating highly active stem cells from human stem cells and highly active stem cells separated thereby
MX2011000993A MX2011000993A (es) 2008-07-28 2009-07-17 Metodo para separar celulas madre activas de celulas madre humanas y celulas madre altamente activas separadas por el mismo.
CN2009801297934A CN102112599A (zh) 2008-07-28 2009-07-17 从人干细胞中分离高活性干细胞的方法及由此分离的高活性细胞
JP2011521015A JP2011529340A (ja) 2008-07-28 2009-07-17 ヒト幹細胞から高活性の幹細胞を分離する方法及びその方法により分離された高活性の幹細胞
CA2732025A CA2732025A1 (en) 2008-07-28 2009-07-17 Method for separating highly active stem cells from human stem cells and highly active stem cells separated thereby

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0073383 2008-07-28
KR1020080073383A KR101175175B1 (ko) 2008-07-28 2008-07-28 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및상기 방법에 의해 분리된 고활성 줄기 세포

Publications (3)

Publication Number Publication Date
WO2010013906A2 WO2010013906A2 (ko) 2010-02-04
WO2010013906A3 true WO2010013906A3 (ko) 2010-05-14
WO2010013906A9 WO2010013906A9 (ko) 2010-07-15

Family

ID=41610818

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/003954 WO2010013906A2 (ko) 2008-07-28 2009-07-17 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및 상기 방법에 의해 분리된 고활성 줄기 세포

Country Status (8)

Country Link
US (1) US20110142809A1 (ko)
EP (1) EP2322602A4 (ko)
JP (1) JP2011529340A (ko)
KR (1) KR101175175B1 (ko)
CN (1) CN102112599A (ko)
CA (1) CA2732025A1 (ko)
MX (1) MX2011000993A (ko)
WO (1) WO2010013906A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110112164A (ko) * 2010-04-05 2011-10-12 서울대학교병원 인간 줄기세포의 활성을 증가시키는 방법
WO2011126264A2 (ko) * 2010-04-05 2011-10-13 서울대학교병원 인간 줄기세포의 활성을 증가시키는 방법
KR101677293B1 (ko) * 2010-04-16 2016-11-17 서울대학교병원 줄기세포의 활성을 증가시키는 방법 및 상기 방법에 의해 제조된 고활성 줄기 세포
JP6401818B2 (ja) * 2016-04-27 2018-10-10 株式会社金太郎ＣｅｌｌｓＰｏｗｅｒ 活性化幹細胞製造方法
MY186453A (en) * 2016-04-27 2021-07-22 Kintarocellspower Co Ltd Method for producing activated stem cells
WO2019074342A1 (ko) * 2017-10-13 2019-04-18 주식회사 나이벡 단백질표지자 grp78을 이용한 고효율 줄기세포의 선별 방법
EP3696547A4 (en) 2017-10-13 2021-09-22 Nibec Co., Ltd. PEPTIDE DERIVATIVE OF GRP78 ALLOWING THE IDENTIFICATION OF HIGH YIELD STEM CELLS

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Also Published As

Publication number Publication date
KR101175175B1 (ko) 2012-08-20
US20110142809A1 (en) 2011-06-16
EP2322602A4 (en) 2012-08-01
KR20100012153A (ko) 2010-02-08
WO2010013906A2 (ko) 2010-02-04
JP2011529340A (ja) 2011-12-08
EP2322602A2 (en) 2011-05-18
WO2010013906A9 (ko) 2010-07-15
MX2011000993A (es) 2011-06-06
CN102112599A (zh) 2011-06-29
CA2732025A1 (en) 2010-02-04

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