WO2009130976A1 - 微細流路および分析用具 - Google Patents
微細流路および分析用具 Download PDFInfo
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- WO2009130976A1 WO2009130976A1 PCT/JP2009/056503 JP2009056503W WO2009130976A1 WO 2009130976 A1 WO2009130976 A1 WO 2009130976A1 JP 2009056503 W JP2009056503 W JP 2009056503W WO 2009130976 A1 WO2009130976 A1 WO 2009130976A1
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- discharge
- liquid
- pair
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Images
Classifications
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
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- G—PHYSICS
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- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y10T137/7303—Control of both inflow and outflow of tank
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
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Definitions
- the present invention relates to a fine flow path for feeding a liquid and an analysis tool.
- a method of analyzing a reaction solution obtained by reacting a specimen with a reagent by an optical method has been performed.
- an analysis tool that provides a reaction field is used.
- an analysis tool in which a fine channel for feeding the sample using a capillary phenomenon is formed is used (for example, see Patent Document 1).
- FIG. 17 shows an example of such a fine flow path.
- the microchannel X shown in the figure includes an inflow port 91, an analysis chamber 92, an exhaust port 93, and an open chamber 94, and is configured so that a sample S such as blood can be fed by capillary action.
- the analysis chamber 92 has a circular cross section and is a place for optically analyzing the concentration of a specific component of the specimen by transmitting light in a state where the specimen is filled.
- the analysis chamber 92 is defined by a pair of side surfaces 92a and 92b facing each other and a pair of surfaces (not shown) that are perpendicular to the side surfaces 92a and 92b and are opposed to each other at a very small distance.
- the open chamber 94 is connected to the analysis chamber 92 through the discharge port 93.
- the open chamber 94 is opened to the atmosphere by a route (not shown).
- the liquid feeding in the fine channel X is as follows. First, a specimen S such as blood is introduced upstream of the fine channel X. This sample S is introduced by capillary action and flows into the analysis chamber 92 from the inlet 91 as shown in FIG. The vicinity of the side surfaces 92a and 92b is a region surrounded by the three surfaces of the pair of surfaces and the side surface 92a or side surface 92b. Capillary force is likely to act on the specimen S, and the driving force is increased. For this reason, the sample S shows a tendency to travel along the side surfaces 92a and 92b.
- the sample S reaches the discharge port 93 only through the side surface 92 a, and the discharge port 93 is blocked by the sample S.
- bubbles Bl are generated near the side surface 92b.
- the light irradiated to the analysis chamber 92 transmits not only the sample S but also the bubbles Bl. Therefore, the specific component of the sample S cannot be analyzed appropriately.
- the present invention has been conceived under the circumstances described above, and an object thereof is to provide a fine channel and an analytical tool capable of appropriately feeding a liquid.
- the fine channel provided by the first aspect of the present invention is sandwiched between an inflow portion located on the upstream side in the flow direction, a discharge portion located on the downstream side in the flow direction, and the inflow portion and the discharge portion.
- An enlarged portion having a cross-sectional area larger than these, and a fine channel through which a fluid is fed, wherein the discharge portion is located on the opposite side in the flow direction with respect to the inflow portion It is characterized by including a pair of outlets.
- the liquid is fed using capillary action.
- the discharge portion further includes a separation portion that is located between the pair of discharge ports and is tapered toward the upstream side in the flow direction.
- the discharge part further includes a stay part that is connected to at least one of the pair of discharge ports on the downstream side in the flow direction and has a larger cross-sectional area than the discharge port. .
- the staying portion is provided with a swelling member that expands when the liquid is absorbed and prevents the liquid from flowing out downstream from the staying portion. Yes.
- the stay portion has a ceiling surface and a bottom surface that are spaced apart from each other in a direction perpendicular to the flow direction, and the bottom surface is spaced from the ceiling surface and the bottom surface.
- An island portion located closer to the center in the opposite direction view, and an enclosure portion that surrounds the island portion in the oppositely opposite direction view and is further away from the ceiling surface than the island portion.
- an additional enlarged portion located on the downstream side in the flow direction of the staying portion, an additional inflow portion that guides liquid from the staying portion to the additional enlarged portion, and And an additional discharge portion including a pair of additional discharge ports that discharge liquid from the additional expansion portion and are located on the opposite side of the flow direction with respect to the additional inflow portion.
- an open chamber that can be opened to the atmosphere is provided between the staying portion and the additional inflow portion.
- the open chamber is connected to a decompression means capable of reducing the pressure in the open chamber.
- the analysis tool provided by the second aspect of the present invention includes the fine channel provided by the first aspect of the present invention, and the enlarged portion is used as a place for analysis.
- FIG. 4 is a cross-sectional view of a principal part taken along line IV-IV in FIG. 3. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. FIG.
- FIG. 9 is a cross-sectional view of a principal part taken along line IX-IX in FIG. 8. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the liquid feeding in the microchannel shown in FIG. It is a principal part top view which shows the other example of a retention part.
- FIG. 13 is an essential part cross-sectional view taken along line XIII-XIII in FIG. 12. It is a principal part top view which shows the flow of the liquid in the retention part shown in FIG. It is a principal part top view which shows the further another example of a retention part. It is a principal part top view which shows the other example of the microchannel based on this invention.
- the analysis tool A of the present embodiment is for performing an analysis on a specific component by an optical method for a specimen S such as blood.
- the analysis tool A is configured to be able to be loaded into an analysis apparatus (not shown). In FIG. 2, a cover 2 to be described later is omitted for convenience of understanding.
- the analysis tool A is supported by a support base 83 provided in the analyzer.
- a rotating shaft 84 is connected to the support base 83 so that the analysis tool A can be freely rotated.
- the light emitting module 81 has, for example, an LED chip, and is a light emitting source that emits light used for optical analysis.
- the light receiving module 82 includes, for example, a silicon photodiode, and receives light from the light emitting module 81 through the analysis tool A.
- the analyzer is configured to be able to analyze a specific component of the sample S depending on the light receiving state of the light receiving module 82.
- the analysis tool A as a whole has a substantially disk shape, and is constituted by a base 1 and a cover 2, and an introduction chamber 3 and a plurality of fine channels 4 are formed. .
- the base 1 is made of a transparent resin material such as polystyrene (PS), polymethyl methacrylate (PMMA), or polydimethylsiloxane (PDMS), and has a disk shape.
- the base 1 is formed with recesses that constitute the introduction chamber 3 and the plurality of fine channels 4.
- the cover 2 is affixed to the base 1, for example, a circular film made of a transparent resin material such as polyethylene terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), or polydimethylsiloxane (PDMS) or It is a disk shape.
- PET polyethylene terephthalate
- PS polystyrene
- PMMA polymethyl methacrylate
- PDMS polydimethylsiloxane
- the introduction chamber 3 is a part for introducing the specimen S such as blood using a dropper or the like, and is constituted by a through hole formed in the cover 2 and a circular recess formed in the base 1.
- the inflow portion 3 is provided with a separation membrane (not shown).
- This separation membrane is interposed, for example, between the base 1 and the cover 2 and is for separating blood cell components in blood.
- a separation membrane for example, a porous material such as paper, foam (foam), woven fabric, non-woven fabric, knitted fabric, membrane filter, glass filter, or gel material is used. be able to.
- the plurality of microchannels 4 are those in which the specimen S introduced from the introduction chamber 3 is fed by capillary action, and a part thereof is used as a place for analysis using an optical technique.
- the plurality of fine flow paths 4 extend radially from the introduction chamber 3. As shown in FIG. 3, the fine channel 4 has an inlet 5, an analysis chamber 6, and a discharge part 7.
- the inlet 5 is a part where the sample S introduced into the introduction chamber 3 flows into the analysis chamber 6.
- the inflow port 5 has a width of about 0.1 mm and a depth of about 0.1 mm.
- the analysis chamber 6 is a place for performing an analysis on the specimen S using an optical technique, and is, for example, a circular portion having a larger cross-sectional area than the front and rear portions in the flow direction.
- the analysis chamber 6 corresponds to an example of an enlarged portion referred to in the present invention.
- the analysis chamber 6 is defined by a top surface 61, a bottom surface 62, and a pair of side surfaces 63a and 63b.
- the top surface 61 and the bottom surface 62 are opposed to each other in the vertical direction in FIG. 4 which is perpendicular to the flow direction, and the pair of side surfaces 63a and 63b are separated in the width direction perpendicular to the vertical direction. Opposite.
- the distance between the top surface 61 and the bottom surface 62, that is, the depth of the analysis chamber 6 is about 0.1 mm. Further, the radius of curvature of the pair of side surfaces 63a and 63b is set to about 0.6 mm, and thereby the approximate diameter of the analysis chamber 6 is set to about 1.2 mm.
- a reagent (not shown) is applied to the analysis chamber 6.
- This reagent is in the form of a dry solid that dissolves when the sample S is supplied, and reacts with a specific component in the sample S to develop a color.
- the analysis tool A for example, a plurality of types of reagents having different components or compositions are prepared so that a plurality of items can be measured.
- the reagent does not necessarily have to be provided in all the analysis chambers 6.
- the application of the reagent is omitted for the analysis chamber 6 used to correct the influence of the color of the specimen.
- a reagent may be applied to an appropriate place other than the analysis chamber 6.
- the discharge part 7 is a part for discharging the sample S from the analysis chamber 6, and includes a pair of discharge ports 71 a and 71 b, a separation part 72, a pair of connection channels 73 a and 73 b, a pair of staying parts 74 a and 74 b, A pair of connection channels 76 a and 76 b and an open chamber 77 are provided.
- the pair of discharge ports 71 a and 71 b are arranged in parallel on the opposite side in the flow direction with respect to the inflow port 5.
- the discharge port 71a is connected to the side surface 63a
- the discharge port 71b is connected to the side surface 63b.
- the separation unit 72 defines a pair of discharge ports 71a and 71b and is tapered toward the upstream in the flow direction.
- the pair of connection flow paths 73a and 73b are connected to the pair of discharge ports 71a and 71b, respectively, and have a width of about 0.05 mm and a depth of about 0.05 mm, for example.
- the pair of retention portions 74a and 74b are circular portions for retaining the discharged specimen S, and are connected to the pair of connection flow paths 73a and 73b, respectively.
- swelling members 75a and 75b are provided in the stay portions 74a and 74b, respectively.
- the swelling members 75a and 75b are made of a material whose volume swells several to several hundred times by absorbing the specimen S.
- a material for example, Aqua Coke (manufactured by Sumitomo Seika), Wonder Gel (manufactured by Kao), Sunwet (manufactured by Sanyo Kasei), Aqua Reserve GP (manufactured by Nippon Synthetic Chemical) may be used.
- the swelling members 75a and 75b are applied so that the upper portions of the stay portions 74a and 74b are hollow.
- the pair of staying portions 74a and 74b are connected to the open chamber 77 via a pair of connecting flow paths 76a and 76b.
- the open chamber 77 is open to the atmosphere via a route (not shown).
- the sample S introduced from the introduction chamber 3 flows into the analysis chamber 6 through the inlet 5 due to capillary action.
- the sample S that has flowed in tends to travel along the pair of side surfaces 63a and 63b. This is because the vicinity of the pair of side surfaces 63a and 64b is a space surrounded on three sides by the top surface 61, the bottom surface 62, and the side surface 63a or the side surface 63b. In such a space, for example, a capillary force acts more strongly than a space surrounded by only two surfaces of the top surface 61 and the bottom surface 62 like the central portion of the analysis chamber 6. For this reason, the sample S is more strongly promoted.
- the side surface 63a and the side surface 63b are rarely the same and rarely have various differences.
- the boundary portion between the pair of side surfaces 63a and 63b and the top surface 61 or the bottom surface 62 is formed as a very small R shape having a predetermined radius of curvature. Since there is a limit to the machining accuracy of such a boundary portion, a slight error occurs in the R shape.
- this treatment may vary depending on the location. Under such circumstances, there may be a considerable difference between the speed at which the specimen S travels along the side surface 63a and the speed at which the specimen S travels along the side surface 63b.
- the sample S When the sample S further travels along the side surface 63a, the sample S reaches the discharge port 71a as shown in FIG. At this time, the sample S tends to be transmitted from the discharge port 71a to the discharge port 71b. However, since the top part of the separation part 72 is a sharp corner part, it exerts a remarkably large resistance against the specimen S that tries to exceed this. For this reason, the sample S does not proceed to the discharge port 71b beyond the separation unit 72. Therefore, the specimen S advances from the discharge port 71a to the connection channel 73a. Also at this time, the specimen S hardly progresses with respect to the side surface 63b.
- the sample S When the sample S reaches the staying portion 74a beyond the connection channel 73a, the sample S is absorbed by the swelling member 75a. Then, as shown in FIG. 9, the swollen swelling member 75 a is in a state of completely closing the staying portion 74 a. Thereby, the force which advances the sample S via the discharge port 71a, the connection flow path 73a, and the retention part 74a will not act. For this reason, as shown in FIG. 10, a force that directs the sample S toward the discharge port 71 b, the connection channel 73 b, and the staying portion 74 b acts on the sample S. The specimen S travels along the side surface 63b and reaches the discharge port 71b as shown in FIG.
- the specimen S reaches the staying part 74b via the connection channel 73b, the staying part 74b is blocked by the swelling of the swelling member 75b.
- the liquid feeding of the sample S in the illustrated fine channel 4 is completed.
- the light from the light emitting module 81 is irradiated to the analysis chamber 6 filled with the sample S, thereby performing analysis by an optical method.
- the sample S flowing into the analysis chamber 6 is discharged from both the pair of discharge ports 71a and 71b. For this reason, even if the sample S reaches the discharge ports 71a and 71b in advance, the remaining sample S and the gas in the analysis chamber 6 can be discharged from the other of the discharge ports 71a and 71b. is there. Therefore, it is possible to avoid large bubbles from remaining in the analysis chamber 6, and the analysis by the optical method can be appropriately performed.
- the specimen S is likely to travel along the pair of side surfaces 63a and 63b where the capillary force acts more strongly. Since the pair of discharge ports 71a and 71b are connected to the side surfaces 63a and 63b, the proceeding sample S can be reliably discharged from the discharge ports 71a and 71b. In addition, the specimen S that has progressed to one of the pair of discharge ports 71 a and 71 b becomes a shape that is blocked by the separation unit 72. For this reason, there is little possibility that both of the discharge ports 71a and 71b are blocked by the sample S that has traveled along one of the pair of side surfaces 63a and 63b. This is suitable for avoiding bubbles remaining in the analysis chamber 6.
- the pair of staying portions 74a and 74b are completely blocked by the swelling members 75a and 75b when the specimen S flows in. For this reason, it is possible to avoid the specimen S discharged from one of the pair of discharge ports 71a and 71b from flowing backward into the analysis chamber 6 from the other of the pair of discharge ports 71a and 71b. This is suitable for preventing bubbles from remaining in the analysis chamber 6. Unlike the present embodiment, a considerable amount of specimen can be retained in the retaining portions 74a and 74b even if the swelling members 75a and 75b are not provided. For this reason, it is possible to delay the flow of the sample S from one of the pair of discharge ports 71a and 71b to the other for a considerable time, and this also avoids remaining bubbles.
- FIG. 12 and FIG. 13 show other examples of the retention portion referred to in the present invention.
- the stay part 74c shown in the figure has a circular shape in plan view, and has a ceiling surface 741 and a bottom surface 742 as shown in FIG.
- the ceiling surface 741 is a flat surface having no level difference and is flush with the upper surfaces of the connection flow paths 73a and 76a.
- the bottom surface 742 is opposed to the ceiling surface 741 as a whole, and includes an island portion 742a and a surrounding portion 742b. As shown in FIG. 12, the island portion 742a is a portion closer to the center of the bottom surface 742, and has a circular shape in plan view in the present embodiment.
- the surrounding part 742b surrounds the island part 742a in a plan view, and has a donut shape in a plan view in the present embodiment. As shown in FIG. 13, the island portion 742 a is relatively close to the ceiling surface 741. On the other hand, the surrounding portion 742b is separated from the ceiling surface 741 as compared to the island portion 742a.
- FIG. 15 shows still another example of the staying portion referred to in the present invention.
- the dwelling portion 74d shown in the figure has a hydrophobic region whose inner surface is subjected to a hydrophobic treatment, and in this embodiment, the ceiling surface and the bottom surface are the hydrophobic regions.
- the liquid S that has flowed from the connection channel 73a is given a resistance force that is prevented from advancing by the hydrophobic region.
- the retention part 74b can delay that the liquid S flows out to the connection flow path 76a located in the downstream. Even with such a configuration, it is possible to delay the flow of the specimen S from one of the pair of outlets 71a and 71b as shown in FIG. 3 to the other for a considerable time, thereby avoiding remaining bubbles. Can do.
- FIG. 16 shows another example of the fine channel according to the present invention.
- the fine channel 4 shown in the figure has a structure in which three analysis chambers 6A, 6B, 6C are connected.
- the liquid feeding using this fine channel 4 will be described below.
- the liquid S is sent from the inflow port 5A to the staying portions 74a and 74b of the discharge portion 7A through the analysis chamber 6A by the same method as the liquid sending described with reference to FIGS.
- the reagent applied in advance reacts with the liquid S.
- the retention parts 74a and 74b of this embodiment are comprised by the comparatively large capacity
- a decompression pump PA as decompression means is connected to the open chamber 77A of the discharge part 7A.
- the liquid S flows into the open chamber 77A.
- the open chamber 77B of the discharge unit 7B on the downstream side of the analysis chamber 6B is opened to the atmosphere. Then, similarly to the liquid feeding described with reference to FIGS. 5 to 11, the liquid S is sent from the inflow port 5B to the staying portions 74a and 74b of the discharge portion 7B through the analysis chamber 6B.
- the analysis chamber 6B for example, a reaction with a reagent different from the reagent in the analysis chamber 6A is performed. Alternatively, in the case where the reagent is not applied to the analysis chamber 6B, the analysis chamber 6B may be used to correct the influence of the color of the specimen.
- a decompression pump PB is connected to the open chamber 77B. When the internal pressure of the open chamber 77B is reduced by the decompression pump PB, the liquid S flows into the open chamber 77B.
- the liquid S is sent from the inlet 5C to the stay sections 74a and 74b of the discharge section 7C through the analysis chamber 6C.
- a decompression pump PC is connected to the open chamber 77C. When the internal pressure of the open chamber 77C is reduced by the decompression pump PC, the liquid S flows into the open chamber 77C.
- the state in which the liquid S is caused to flow into the open chamber 77C is a state in which preparation for further feeding the liquid S further downstream is completed. Therefore, in addition to the analysis chambers 6A, 6B, and 6C, it is possible to send the liquid S to another analysis chamber and perform another inspection.
- the fine channel and the analysis tool according to the present invention are not limited to the above-described embodiment.
- the specific configuration of each part of the fine channel and the analysis tool according to the present invention can be varied in design in various ways.
- the analysis chamber 6 corresponding to the enlarged portion referred to in the present invention is not limited to a circular one, but may be any shape as long as the sample S can be appropriately fed by an optical technique while feeding the sample S by capillary action. Good.
- the pair of discharge ports 71a and 71b are preferably partitioned by the separation portion 72, but the present invention is not limited to this.
- the pair of staying portions 74 a and 74 b and the swelling members 75 a and 75 b provided on these are preferable for preventing the backflow of the sample S, but the sample S flows back downstream of the discharge portion 7 in the microchannel 4. If a difficult mechanism is employed, a configuration without these may be used.
- Analytical tool A is an example of one that performs liquid feeding using the fine flow path 4, and may be one that performs analysis other than analysis using an optical technique, for example.
- the specimen S is not limited to those using blood.
- the fine flow channel according to the present invention is suitable for use in analysis of a very small amount of blood, but is not limited to this, and is used for various applications having a part for sending liquid via an enlarged part. Can do.
- the fine channel according to the present invention is not limited to the one using the capillary phenomenon as the driving force of the liquid feeding, for example, a configuration in which the fluid is fed by a relatively small pressure difference generated before and after the flow direction. Even so, it is possible to expect the effect of suppressing the remaining bubbles in the enlarged portion and smoothly feeding the liquid.
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Abstract
Description
上記滞留部は、疎水領域を有している、請求項4に記載の微細流路。
Claims (11)
- 流れ方向上流側に位置する流入部と、
流れ方向下流側に位置する排出部と、
上記流入部および上記排出部に挟まれており、これらよりも断面積が大である拡大部と、
を備え、流体が送液される微細流路であって、
上記排出部は、上記流入部に対して上記流れ方向において反対側に位置する1対の排出口を含むことを特徴とする、微細流路。 - 毛細管現象を利用して上記液体が送液される、請求項1に記載の微細流路。
- 上記排出部は、上記1対の排出口の間に位置し、かつ上記流れ方向上流側に向かってテーパ状とされた分離部をさらに有する、請求項1に記載の微細流路。
- 上記排出部は、上記1対の排出口の少なくともいずれかの流れ方向下流側に繋がり、かつ上記排出口よりも断面積が大である滞留部をさらに備える、請求項1に記載の微細流路。
- 上記滞留部には、上記液体を吸収したときに膨張することにより、上記滞留部より下流側に上記液体が流出することを阻止する膨潤部材が設けられている、請求項4に記載の微細流路。
- 上記滞留部は、上記流れ方向と直角である方向において互いに離間対向する天井面および底面を有しており、
上記底面は、上記天井面および上記底面が離間対向する方向視において中央寄りに位置する島部と、上記離間対向する方向視において上記島部を囲み、かつ上記島部よりも上記天井面に対して離間する包囲部と、を有する、請求項4に記載の微細流路。 - 上記滞留部は、疎水領域を有している、請求項4に記載の微細流路。
- 上記滞留部の上記流れ方向下流側に位置する追加の拡大部と、
上記滞留部からの液体を上記追加の拡大部へと導く追加の流入部と、
上記追加の拡大部から液体が排出され、かつ上記追加の流入部に対して上記流れ方向反対側に位置する1対の追加の排出口を含む追加の排出部と、をさらに備える、請求項1に記載の微細流路。 - 上記滞留部と上記追加の流入部との間には、大気開放状態とすることが可能な開放室が設けられている、請求項7に記載の微細流路。
- 上記開放室が、上記開放室内の圧力を減圧させることが可能な減圧手段に繋げられている、請求項8に記載の微細流路。
- 請求項1に記載の微細流路を備え、
上記拡大部が、分析の場として用いられることを特徴とする、分析用具。
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CN2009801144765A CN102016598B (zh) | 2008-04-25 | 2009-03-30 | 微细流路及分析用具 |
US12/988,602 US8398937B2 (en) | 2008-04-25 | 2009-03-30 | Microchannel and analyzing device |
EP09733832.1A EP2270515B1 (en) | 2008-04-25 | 2009-03-30 | Microchannel and analyzing device |
JP2010509125A JP5255628B2 (ja) | 2008-04-25 | 2009-03-30 | 微細流路および分析用具 |
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EP2213364A1 (en) * | 2009-01-30 | 2010-08-04 | Albert-Ludwigs-Universität Freiburg | Phase guide patterns for liquid manipulation |
TWI385030B (zh) * | 2010-12-22 | 2013-02-11 | Univ Nat Chiao Tung | 具有氣泡之微流體系統及其氣體放電方法與氣體反應方法 |
EP2896457B1 (en) * | 2014-01-15 | 2017-08-23 | IMEC vzw | Microstructured micropillar arrays for controllable filling of a capillary pump |
JP2016217929A (ja) * | 2015-05-22 | 2016-12-22 | セイコーエプソン株式会社 | センサー用ゲルおよびセンサー |
US9757728B2 (en) * | 2016-01-26 | 2017-09-12 | Lidong Qin | Microfluidic aliquoting for single-cell isolation |
WO2020066609A1 (ja) * | 2018-09-28 | 2020-04-02 | ウシオ電機株式会社 | 細胞培養チップ |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0694724A (ja) * | 1985-08-05 | 1994-04-08 | Biotrack Inc | 粒子含有流体の消耗を検出することができる制御装置 |
JP2004150804A (ja) | 2002-10-28 | 2004-05-27 | Arkray Inc | 分析用具および分析装置 |
JP2005181295A (ja) * | 2003-10-23 | 2005-07-07 | F Hoffmann La Roche Ag | 流動トリガー装置 |
WO2006098370A1 (ja) * | 2005-03-16 | 2006-09-21 | Nec Corporation | 流路の実効的な通過時間の調整機構を具える遅延回路、マイクロチップ、およびその作製方法 |
WO2006106608A1 (ja) * | 2005-04-04 | 2006-10-12 | Matsushita Electric Industrial Co., Ltd. | 液体均一化装置およびそれを用いた分析装置 |
WO2007013562A1 (ja) * | 2005-07-29 | 2007-02-01 | Arkray, Inc. | 分析用具 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6637463B1 (en) * | 1998-10-13 | 2003-10-28 | Biomicro Systems, Inc. | Multi-channel microfluidic system design with balanced fluid flow distribution |
US8231845B2 (en) * | 2000-10-25 | 2012-07-31 | Steag Microparts | Structures for uniform capillary flow |
WO2003025547A1 (en) * | 2001-09-21 | 2003-03-27 | Biomedlab Corporation | Method and device for screening analytes using surface plasmon resonance |
WO2006098696A1 (en) * | 2005-03-16 | 2006-09-21 | Attogenix Biosystems Pte Ltd. | Methods and device for transmitting, enclosing and analysing fluid samples |
-
2009
- 2009-03-30 CN CN2009801144765A patent/CN102016598B/zh active Active
- 2009-03-30 JP JP2010509125A patent/JP5255628B2/ja active Active
- 2009-03-30 US US12/988,602 patent/US8398937B2/en active Active
- 2009-03-30 EP EP09733832.1A patent/EP2270515B1/en active Active
- 2009-03-30 WO PCT/JP2009/056503 patent/WO2009130976A1/ja active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0694724A (ja) * | 1985-08-05 | 1994-04-08 | Biotrack Inc | 粒子含有流体の消耗を検出することができる制御装置 |
JP2004150804A (ja) | 2002-10-28 | 2004-05-27 | Arkray Inc | 分析用具および分析装置 |
JP2005181295A (ja) * | 2003-10-23 | 2005-07-07 | F Hoffmann La Roche Ag | 流動トリガー装置 |
WO2006098370A1 (ja) * | 2005-03-16 | 2006-09-21 | Nec Corporation | 流路の実効的な通過時間の調整機構を具える遅延回路、マイクロチップ、およびその作製方法 |
WO2006106608A1 (ja) * | 2005-04-04 | 2006-10-12 | Matsushita Electric Industrial Co., Ltd. | 液体均一化装置およびそれを用いた分析装置 |
WO2007013562A1 (ja) * | 2005-07-29 | 2007-02-01 | Arkray, Inc. | 分析用具 |
Non-Patent Citations (1)
Title |
---|
See also references of EP2270515A4 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011163986A (ja) * | 2010-02-10 | 2011-08-25 | Fujifilm Corp | マイクロ流路デバイス |
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JPWO2009130976A1 (ja) | 2011-08-18 |
US8398937B2 (en) | 2013-03-19 |
EP2270515B1 (en) | 2019-05-08 |
US20110038766A1 (en) | 2011-02-17 |
CN102016598B (zh) | 2013-10-30 |
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