WO2009093384A1 - ポリヌクレオチド及びポリヌクレオチド類似体並びにこれらを用いた遺伝子発現制御方法 - Google Patents
ポリヌクレオチド及びポリヌクレオチド類似体並びにこれらを用いた遺伝子発現制御方法 Download PDFInfo
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- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
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- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N2310/32—Chemical structure of the sugar
- C12N2310/323—Chemical structure of the sugar modified ring structure
- C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
Definitions
- the present invention relates to polynucleotides and polynucleotide analogs. More specifically, polynucleotides and polynucleotide analogs having a base sequence complementary to at least a part of the cis element of mRNA and capable of controlling the expression of gene products, and a method for controlling gene expression using this polynucleotide and the like About.
- Biological homeostasis is maintained by the function of various genes related to various biological reactions such as cell differentiation and proliferation, neurotransmission, and immune response, and appropriately changing their functions in response to stimuli from inside and outside the body. ing.
- the expression site, expression level, and expression time of each gene must be precisely controlled, and if any gene deviates from normal control and is expressed in excess or under, homeostasis collapses, It leads to pathological conditions such as cancer, chronic inflammation, and autoimmune diseases. Therefore, in order to prevent, ameliorate, or treat diseases such as cancer, chronic inflammation, and autoimmune diseases, the expression of genes related to the disease state is appropriately controlled by inhibiting or promoting, and the desired expression state is induced. It is necessary to do.
- Intracellular gene expression is controlled by controlling the amount of mRNA transcribed, the stability of the transcribed mRNA, the amount of translation into the protein, and the stability of the protein itself at each stage of mRNA transcription and translation. Has been done. To date, various gene expression control methods targeting each of these control stages have been developed.
- an “antisense method” that introduces a single-stranded RNA or DNA (antisense strand) complementary to a target gene mRNA (sense strand) into a cell and selectively inhibits the translation of the sense strand into a protein.
- the antisense method has high gene selectivity and acts directly on the target gene mRNA, so that it is expected to be highly effective with low toxicity.
- RNA interference is an expected gene expression control method along with the antisense method.
- RNAi is a double-stranded RNA (dsRNA) complementary to the target gene mRNA or a small interfering RNA (short interfering RNA; siRNA) produced by the degradation of this dsRNA by an enzyme called Dicer belonging to the RNase III family.
- siRNA small interfering RNA
- RISC RNA Induced Silencing Complex
- the stability and translation amount of mRNA are controlled by a specific sequence called “cis-element” of mRNA and “cis-element binding factor” that binds to this specific sequence.
- the cis element is defined as a region that is present in the 5 ′ and 3 ′ untranslated regions of DNA and RNA, and that is involved in the expression control of the gene encoded by the DNA strand or RNA strand.
- a “cis element binding factor” functions as a trans-acting factor that binds to a cis element of a gene and positively or negatively regulates the expression of the gene.
- Promoters and enhancers have been well known as cis elements present in DNA.
- Typical promoters include TATA box, CAT box, and Sp1, which have universal transcription factors bound as cis element binding factors to control gene expression at the transcription level.
- the cis element present in mRNA is involved in the control of mRNA stability and the amount of translation into protein, and is an important factor for determining the expression level of the gene product (protein) encoded by mRNA.
- ARE AU-rich element
- ARE is a base sequence of about 10 to 150 bps rich in adenosine and uridine, and is abundant in the 3 ′ untranslated region (3 ′ UTR) of mRNA. ARE was initially found as a region where the nucleotide sequence of “AUUUA” frequently overlaps in the 3 ′ UTR of cytokines and lymphokines. It is estimated that ARE is present in 5 to 8% of all genes at present, and it is considered that ARE is present in many genes involved in the maintenance of homeostasis (see Non-Patent Document 1).
- ARE binds to ARE as a trans-acting factor, and mRNA stability and translation amount are controlled to be positive and negative (mainly negative) (see Non-Patent Document 2 and Non-Patent Document 3). Furthermore, microRNA (miRNA) is also bound to ARE, and the same control is performed (see Non-Patent Document 4 and Non-Patent Document 5).
- miRNA microRNA
- LNA Locked Nucleic Acid
- Nucleosides in natural nucleic acids can take two types of conformations: N-type and S-type. Due to this “fluctuation” between conformations, the double strands formed between DNA and DNA, between RNA and RNA, and between DNA and RNA are not necessarily thermodynamically stable.
- 2 ', 4' -LNA Bridged Nucleic Acid; synonymous with BNA
- the gene expression control methods such as the antisense method and RNAi described above can be achieved by inhibiting the translation of the target gene mRNA or degrading the mRNA based on the sequence specificity of the antisense strand, dsRNA, or siRNA. It negatively regulates the expression of gene products. That is, these methods cannot positively control the expression of the target gene product to increase the expression level.
- a main object of the present invention is to provide a gene expression control method for controlling the expression of a target gene, which can positively control the expression of a target gene product.
- the present invention provides a nucleotide sequence complementary to at least a part of the cis element of mRNA and a part of the untranslated region on the 5 ′ side and / or 3 ′ side of the cis element.
- a cis element to the cis element by specifically binding to at least a part of the cis element based on the sequence specificity of the base sequence complementary to the untranslated region.
- This polynucleotide or polynucleotide analog selectively inhibits the binding of a cis element binding factor to the cis element, thereby selectively stabilizing the mRNA and promoting the translation of the gene product or destabilizing the gene product.
- Inhibits translation of The cis element and the cis element binding factor that are the targets of inhibition of binding by the polynucleotide or the polynucleotide analog can be particularly an AU rich element and an AU rich element binding factor.
- the above-mentioned polynucleotide analogue comprises LNA.
- the present invention also provides a gene product expression enhancer or suppressor containing the above-mentioned polynucleotide and / or polynucleotide analog, or containing an expression vector capable of expressing the polynucleotide, and further the gene product expression enhancer or A pharmaceutical composition containing an inhibitor as an active ingredient is provided.
- the present invention relates to the use of the above-mentioned polynucleotide and / or polynucleotide analog for the production of a target gene product expression enhancer or inhibitor, or an expression vector capable of expressing the polynucleotide, and in particular, to express a polynucleotide. Possible expression vectors are also provided.
- the present invention provides a polynucleotide or polynucleotide analog that inhibits the binding of ZFP36L1 and / or ZFP36L2 to the AU-rich element, wherein the mRNA is LDLR mRNA.
- This polynucleotide and polynucleotide analogue inhibits the binding of a cis element binding factor to ARE1 (2790 to 2797 bases from the 5 ′ end of the LDLR mRNA base sequence shown in SEQ ID NO: 4).
- the polynucleotide and the polynucleotide analogue can include the base sequence shown in any of SEQ ID NOs: 1 to 3 and 6 to 14, or a base sequence substantially identical to the base sequence.
- the present invention also provides an LDLR expression enhancer containing any one or more of the above polynucleotides and / or polynucleotide analogues or containing an expression vector capable of expressing one or more of the polynucleotides.
- a pharmaceutical composition for preventing, ameliorating or treating hyperlipidemia or hyperlipidemia-related disease or Alzheimer's disease, comprising this LDLR expression enhancer as an active ingredient is provided.
- the hyperlipidemia-related disease can be one or more diseases selected from the group consisting of arteriosclerosis, hypertension, cerebral infarction, myocardial infarction, angina pectoris, diabetes, obesity, and cancer.
- the present invention also provides an expression vector capable of expressing the polynucleotide.
- a gene expression control method characterized by controlling the expression of a target gene product by inhibiting the binding of a cis element binding factor to the cis element using a polynucleotide and / or a polynucleotide analog.
- Polynucleotide refers to nucleotides linked in a chain by phosphodiester bonds via phosphoric acid. More specifically, it means a nucleic acid chain (DNA chain or RNA chain) made of a conventional nucleic acid (DNA or RNA). Nucleoside is a compound in which a nucleoside is bound to phosphoric acid, and a nucleoside is a compound in which a purine base such as adenine or guanine, a pyrimidine base such as thymine, cytosine or uracil and a sugar (ribose) are bound.
- the base has so-called complementarity, and forms a base pair by hydrogen bonding between adenine / thymine (uracil) and guanine / cytosine. Therefore, two polynucleotides (DNA / DNA, DNA / RNA, RNA / RNA) having complementary base sequences bind with high affinity by hydrogen bonding between base pairs to form a double strand.
- polynucleotides those of several nucleotides to several tens of nucleotides are particularly called oligonucleotides.
- Polynucleotide analog means a polymer having a “base sequence-like structure” that mimics the base sequence of a polynucleotide, that is, a structure in which bases are bonded to a basic molecular chain formed by a sugar chain.
- the “base sequence-like structure” of the polynucleotide analog does not have to be a structure in which the base itself is arranged. Instead of the base, each base of adenine, guanine, cytosine, and uracil is recognized and complemented. It may be a structure in which compounds capable of binding to are bonded to the basic molecular chain and arranged.
- base sequence includes a “base sequence-like structure” possessed by a polynucleotide analog in addition to a base sequence possessed by a polynucleotide.
- concept of “length (number of bases)” includes “the number of compounds” of the above compounds arranged in the basic molecular chain of the polynucleotide analog in addition to the number of bases of the polynucleotide. To do.
- the basic molecular chain of the polynucleotide analog does not need to be formed by a phosphodiester bond of ribose.
- ribose having a modified structure such as the above-mentioned LNA, or sugars other than ribose bound, or these May be bonded by a chemical bond other than a phosphodiester bond.
- the basic molecular chain is not particularly limited as long as it is a molecular chain capable of supporting the above base sequence-like structure.
- a polynucleotide analog has a structure in which the above base sequence-like structure is supported by this basic molecular chain, and binds with high affinity to mRNA having a base sequence complementary to the base sequence-like structure. , Which can form a double strand.
- “Cis element” means a region that is present in the 5 ′ and 3 ′ untranslated regions of mRNA and is involved in the expression control of the gene encoded by the mRNA chain.
- the “cis element binding factor” widely includes proteins and miRNAs that bind to this cis element and positively or negatively control gene expression.
- AU rich element means a base sequence of about 10 to 150 bps rich in adenosine and uridine, which is abundant in 3′UTR of mRNA, and is involved in mRNA stability and translational control.
- AREs are tentatively classified into the following three groups, and the present invention includes at least these three groups of AREs (see Non-Patent Document 2 above).
- Region containing several copies of “AUUUA pentamer” in uridine-rich sequence ARE (I)
- Region containing at least two or more overlapping “UUAUUUA (U / A) (U / A) nonamers” ARE II
- a region that does not contain "AUUUA pentamer” but is rich in uridine ARE III.
- the “AU-rich element binding factor” widely includes proteins and miRNAs that bind to the ARE and control the expression of the gene encoded by the mRNA chain positively or negatively.
- AUF1, HuR, Hel-N1, HuD, TTP, BRF1, TIA-1, KSRP, GUG-BP2, Nucleotin, TINO, PAIP2, ZFP36L1, and ZFP36L2 are contained at least as miRNA.
- the present invention provides a gene expression control method for controlling the expression of a target gene, which can positively control the expression of a target gene product.
- FIG. 1 is a schematic diagram for explaining the regulation mechanism of mRNA stability and the amount of translation into protein by the cis element and cis element binding factor.
- a cis-element binding factor binds to a cis-element present in the 5 ′ and 3 ′ untranslated regions (5′UTR / 3′UTR) of mRNA as a single factor or a complex consisting of multiple factors, and the mRNA.
- the expression of the gene encoded by is controlled positively and negatively.
- cis element binding factors inhibit and stabilize mRNA degradation and promote translation into proteins to enhance gene expression.
- mRNA degradation is promoted to destabilize, and protein expression is inhibited by inhibiting protein translation.
- AUF1 (AU ⁇ binding factor 1), which is an AU rich element binding factor, binds to ARE as a complex containing 3′-5 ′ exoribonuclease and promotes degradation of mRNA (see Non-Patent Document 3 above). ).
- polynucleotide and polynucleotide analogue have a base sequence complementary to a cis element of mRNA, and bind to at least a part of the cis element, thereby binding the cis element. It inhibits positive and negative expression control functions by factors.
- the structure and function of the polynucleotide and polynucleotide analog according to the present invention will be described in detail.
- FIG. 2 is a schematic diagram for explaining an embodiment of a polynucleotide and a polynucleotide analogue according to the present invention.
- a polynucleotide and a polynucleotide analogue (hereinafter also simply referred to as “polynucleotide etc.”) indicated by reference symbol P 1 are cis elements existing in the 5 ′ and 3 ′ untranslated regions of mRNA.
- a region “cCIS” having a complementary base sequence in the region and a region “cUTR” having a base sequence complementary to the 3′-side untranslated region (3′-side UTR) of this cis element are continuously 2 It consists of two areas.
- cCIS and cUTR when the polynucleotide or the like P 1 is a polynucleotide, a region consisting of DNA or RNA, respectively having a nucleotide sequence complementary to the nucleotide sequence of the cis element and 3'-side UTR.
- the polynucleotide or the like P 1 is a polynucleotide analogue, CCIS and cUTR, respectively (1) a nucleotide sequence complementary to the nucleotide sequence of the cis element and 3'-side UTR, or (2) the nucleotide sequence Either a base sequence-like structure in which a compound that can be mimicked, recognizes each base of adenine, guanine, cytosine, and uracil in the base sequence of the cis element and 3′-side UTR, and can bind complementarily is arranged, or A region having both.
- CUTR polynucleotide such P 1 is the polynucleotide or the like P 1, and functions to bind to specific cis-elements on a particular gene mRNA.
- a specific gene mRNA polynucleotide like P 1 is bonded and a specific cis element respectively assumed as "target gene mRNA" and "target cis element”.
- CUTR a provided as a nucleotide sequence complementary to the 3'-side UTR of the target cis element
- CUTR Polynucleotide like P 1 is complementary to the 5'-side UTR of target cis element base It is also possible to design as an array.
- Each cis element such as AU rich element (ARE) and Histone mRNA 3 ′ UTR stem loop element has a characteristic base sequence.
- ARE AU rich element
- Histone mRNA 3 ′ UTR stem loop element has a characteristic base sequence.
- one group of AREs (ARE I) has a base sequence characterized by 6 bases of “AUUUA”.
- Each cis element is present on the mRNA of a plurality of genes as such a specific base sequence, and may be present at a plurality of different positions on the same mRNA. Therefore, the cCIS of P 1 such as a polynucleotide can bind to a plurality of cis elements on a plurality of gene mRNAs having the same specific base sequence in addition to the target cis element of the target gene mRNA based on their complementarity. .
- the 3 ′ UTR of the target cis element has a specific base sequence depending on the target cis element. That is, the 3′-side UTR of the target cis element is present at a different position on the same mRNA and has a different base from the 3′-side UTR of the cis element other than the target having the same specific base sequence as the target cis element. It is an array. And the 3 ′ UTR of this target cis element is naturally a nucleotide sequence different from the 3 ′ UTR of the cis element having the same specific base sequence as the target cis element present on the gene mRNA other than the target gene. .
- CUTR polynucleotide such P 1 is a can specifically bind only to the 3'-side UTR of the target cis element based on the complementarity (sequence specificity) for the 3'-side UTR of the target cis element . Then, the specific binding of this CUTR, in the polynucleotide or the like P 1, the cCIS continuous to CUTR, they are possible to specifically bind only to the target cis element.
- the length of cUTR polynucleotides such as P 1 is between the 3'-side UTR of the target cis element, forming a double-stranded by affinity similar affinity or to by hydrogen bonds between base pairs Therefore, the length should be such that a sufficient binding force can be exerted. Further, the length of cUTR polynucleotides such as P 1 is the length that can exhibit sequence specificity to the nucleotide sequence of the 3'-side UTR of target cis element.
- a specific double strand is formed only with a nucleic acid strand containing a target nucleic acid base sequence under predetermined double strand formation conditions (hybridization conditions). Designing polynucleotides that suppress non-specific binding to other nucleic acids has been carried out.
- cUTR of P 1 such as a polynucleotide in the present invention
- an optimal length can be determined to exhibit sufficient binding force and sequence specificity based on such a conventionally known polynucleotide design method. .
- the length of cUTR polynucleotides such as P 1 is a at least one base or more, preferably 2 bases or more, more preferably 3 bases or more, more preferably 4 bases or more and.
- the polynucleotide or the like P 1 is a polynucleotide consisting of DNA or RNA
- suitable length of cUTR polynucleotides such as P 1 may differ if the polynucleotide or the like P 1 binding force and sequence characteristics comprising high LNA is compared with a case consisting of DNA or RNA, the length of cUTR may be shorter.
- the bonding force and sequence specificity of cUTR polynucleotide such P 1 is, and is also influenced by the length of the full length of the polynucleotide or the like P 1.
- the polynucleotide or the like P 1 can specifically bind cCIS to the target cis element of the target gene mRNA. Therefore, the use of the polynucleotide or the like P 1, may be to inhibit the binding of cis element binding factor to the target cis element competitively. Then, by inhibiting the binding of the cis element binding factor to the target cis element, it is possible to inhibit the mRNA expression control function of the cis element binding factor to enhance or suppress the expression of the target gene.
- the length of cCIS polynucleotides such as P 1 has a base complementary to at least a portion of the target cis element, bonded to a length capable of inhibiting the binding of the cis element-binding factor to the target cis element.
- the length of cCIS polynucleotides such as P 1 is a at least one base or more, preferably 2 bases or more, more preferably 3 bases or more, more preferably 4 bases or more and. It is considered that cCIS can effectively inhibit the binding of a cis element binding factor as its length increases. However, the longer length of the CCIS, there is a possibility that non-specific binding of the polynucleotide or the like P 1 increases, also occurs synthesis cost problems.
- the polynucleotide or the like P 1 is a polynucleotide consisting of DNA or RNA
- a polynucleotide analogue consisting LNA like that suitable lengths of cCIS polynucleotides such as P 1 is different
- the bonding force cCIS polynucleotides such as P 1 is a point that is also influenced by the length of the full length of the polynucleotide or the like P 1, as described above.
- Table 1 summarizes the functions of the cis element-binding factor that binds to the target cis element and which, the effects of when the inhibit these bonds by the polynucleotide or the like P 1.
- the target gene mRNA can be selectively destabilized to inhibit the translation of the target gene product and suppress the expression of the target gene.
- the gene expression enhancing or inhibiting effect by arbitrarily designing the nucleotide sequence of cCIS and cUTR polynucleotides such P 1, it is possible to obtain the desired gene as a target gene. That is, first, a specific cis element in a gene mRNA that is subject to expression control is determined, and a cCIS complementary to the base sequence is designed using this cis element as a target cis element. Then, to design complementary cUTR the nucleotide sequence of the 3'-side UTR of the target cis-element, constituting the polynucleotide or the like P 1. Thus, only by binding a polynucleotide or the like P 1 target cis element of the target gene mRNA to expression control target, to obtain a gene expression enhancing or inhibiting effect as shown in "Table 1" for the target gene Can do.
- the base sequence of cCIS and cUTR are for each target cis element and its 3'UTR nucleotide sequence completely need not be complementary, to the target cis element and its 3'UTR As long as it can bind with high affinity to form a double strand and inhibit the binding of the cis element binding factor to the target cis element, one or more bases in the base sequence of the target cis element and its 3 ′ UTR And a non-complementary base (or compound).
- a base sequence is referred to as a “substantially identical base sequence”.
- the “substantially identical base sequence” means that the target cis element and its 3 ′ UTR bind with high affinity to form a double strand, and the cis element binding factor to the target cis element Obtained by deleting, adding, substituting, or inserting one or more (preferably within several) bases while maintaining the same function, for nucleotide sequences such as polynucleotides that have the function of inhibiting the binding of The modified base sequence is meant.
- the total length of the polynucleotide or the like P 1 is determined by the total length of the cUTR and cCIS described above.
- the length of cUTR is a length that can exert sufficient binding force and sequence specificity to the 3 ′ UTR of the target cis element
- the length of cCIS is a base complementary to at least a part of the target cis element. It has a length that can bind to and inhibit the binding of a cis element binding factor.
- the lengths of cUTR and cCIS of P 1 such as a polynucleotide are each at least 1 base, preferably 2 bases or more, more preferably 3 bases or more, and further preferably 4 bases or more.
- the total length of the polynucleotide or the like P 1 is a at least 2 bases or more, preferably 4 bases or more, more preferably 6 nucleotides or more, more preferably 8 or more nucleotides.
- the total length of the polynucleotide or the like P 1 is, as its length is long, it is possible to increase the avidity for the target cis element and its 3'UTR. However, the overall length is long, there is a possibility that non-specific binding of the polynucleotide or the like P 1 increases, also occurs synthesis cost problems.
- the desired length of the polynucleotide is several bases to several tens of bases in order to avoid such non-specific binding and synthesis cost problems. Desirably, it is about 10 to 30 bases.
- the total length of the polynucleotide or the like P 1 in the present invention can be determined as appropriate in consideration of such conventionally known polynucleotide design criteria. In the case where the polynucleotide or the like P 1 binding force and sequence characteristics comprising high LNA, the length of the overall length may be shorter than the above range.
- Polynucleotide or the like according to the present invention in FIG. 2 (B), the like, such as a polynucleotide indicated at P 2, structure having regions cCIS having a base sequence complementary to the entire length of the target cis element of the target gene mRNA It may be.
- a polynucleotide such as P 2 in addition to cUTR having a nucleotide sequence complementary to the 3'-side UTR of the target cis element may have a complementary cUTR2 the 5'-side UTR.
- the length of cUTR2 polynucleotides such as P 2 are sufficient to form between the 5 'side UTR of the target cis element, a double-stranded by affinity similar affinity or to by hydrogen bonds between base pairs The length is such that a sufficient bonding force can be exerted. Further, the length of cUTR2 polynucleotides such as P 2 is a length capable of exhibiting sequence specificity to the nucleotide sequence 5'-side UTR of target cis element.
- the length of the cUTR polynucleotides such as P 1 described above be at least one base or more, preferably 2 bases or more, more preferably 3 bases or more, more preferably 4 bases or more .
- the polynucleotide or the like P 2 by the cUTR and CUTR2 may be ensured sequence specificity for the target cis element. Therefore, the length of cUTR and cUTR2 polynucleotides such as P 2 may be designed shorter than the length of cUTR polynucleotides such P 1.
- the polynucleotide or the like according to the present invention in FIG. 2 (C), the so such polynucleotide indicated at P 3, a nucleotide sequence complementary to the two cis elements 1, 2 present in the target gene mRNA It may be configured to have the regions cCIS1 and cCIS2 having.
- cCIS1 and cCIS2 are connected by cUTR.
- This cUTR is a 5 ′ UTR of cis element 1 and has a base sequence complementary to the full length of the UTR that is the 3 ′ UTR of cis element 2.
- the cis element 1 and the cis element 2 may be one type of cis element (for example, both ARE), or may be different types of cis elements (for example, one is ARE and the other is IRES).
- the length of cCIS1, cCIS2 polynucleotide such P 3 has a base complementary to at least a portion of the target cis element, and a length capable of inhibiting the binding of cis element binding factor by binding to the target cis element .
- CCIS1 the length of cCIS2 polynucleotides such as P 3, as well as the length of the cCIS polynucleotides such P 1, comprising at least one base or more, preferably 2 bases or more, more preferably 3 More than base, more preferably more than 4 bases.
- the polynucleotide or the like according to the present invention has a cCIS complementary to at least a part of the target cis element, and is complementary to the UTR on the 5 ′ side, 3 ′ side, or both sides of the target cis element. (See FIGS. 2A to 2C).
- the polynucleotide or the like according to the present invention can target two or more cis elements of the target gene mRNA (see FIG. 2 (C)).
- cCIS and cUTR are each of adenine, guanine, cytosine and uracil in the base sequence of cis element and 3′-side UTR. As described above, it is possible to make a region having a base sequence-like structure in which compounds capable of distinguishing and recognizing bases and binding complementarily are arranged.
- a basic molecular chain formed by a phosphodiester bond of ribose is combined with a basic molecular chain in which ribose is modified or a sugar other than ribose.
- a basic molecular chain or a basic molecular chain in which these are bonded by a chemical bond other than a phosphodiester bond can be used.
- a basic molecular chain in which ribose is modified a basic molecular chain in which a sugar other than ribose is bound, or a basic molecular chain in which these are bound by a chemical bond other than a phosphodiester bond, for example, PNA, Morpholino, 2'-O-methyl-RNA, thio DNA, thio RNA, LNA and the like can be used.
- Fig. 3 shows the binding structure of PNA (B) and Morpholino (C).
- A shows the DNA binding structure.
- the PNA shown in Fig. 3 (B) is an example of a basic molecular chain in which the basic molecular chain of a polynucleotide analog is synthesized by something other than a sugar and is bound by "-C (O) -NH-" (amide bond).
- Morpholino shown in (C) is an example of a basic molecular chain obtained by synthesizing a basic molecular chain other than sugar and bonding it with “—P (O) —O—” (phosphomonoester bond).
- the polynucleotide analogue according to the present invention can be synthesized by using two or more of these, or as a hybrid chain composed of these and RNA or DNA.
- LNA having a modified ribose structure can be preferably used.
- Oligonucleotide analogues synthesized by LNA have no “fluctuation” between the two types of N-type and S-type conformations found in oligonucleotides synthesized with conventional natural nucleic acids, and thus have the ability to bind to mRNA. Is extremely high.
- the cCIS more firmly can be coupled to the target cis element, the binding of the cis element binding factor to the target cis element effectively It becomes possible to inhibit.
- oligonucleotide analogues synthesized by LNA are also excellent in sequence specificity.
- oligonucleotide analogs P 1 can be coupled to the 3'-side UTR more specificity higher target cis element of CUTR, you are possible to enhance the selectivity for the target element of an oligonucleotide analogue P 1 Become.
- the oligonucleotide analog synthesized by LNA has high heat resistance and excellent nuclease resistance, it has high stability when used as a gene product expression enhancer or inhibitor described below. And the effect of the gene product expression enhancer or suppressor can be enhanced.
- the LNA, the 2 'position and 4'-position of the ribose "- O - CH 2 -" cross-linked with 2 to fix the conformation N-type', 4 'other-LNA, con by various crosslinking methods LNA with fixed formation can be used.
- the target cis element targeted by the polynucleotide according to the present invention is, for example, AU rich element, Histone mRNA 3 ′ UTR stem loop element, Internal ribosome entry site (IRES), A2RE element , ZIPCODE element, Iron response element (IRE), Cytoplasmic polyadenylation (CPE), Nanos translational control, Amyloid precursor protein element (APP), Translational regulation element (TGE) / direct repeat element (DRE), Bruno element (BRE), 15 -lipoxygenase differentiation control element (15-LOX-DICE), G-quartet element, Adh mRNA down-regulation element, Barley yellow dwarf virus, GLUT1 mRNA-stability control element, Msl-2 3 UTR control element, Msl-2 5 UTR control element, Ribosomal S12 mRNA translational control element, Selenocysteine insertion sequence type 1 (SECIS-1), Selenocysteine insertion sequence
- the target cis element can be particularly an AU rich element (ARE).
- ARE and ARE binding protein mainly negatively control mRNA stability and translation amount. Therefore, by inhibiting the binding of ARE-binding protein to ARE by the polynucleotide according to the present invention, and inhibiting this negative control function, mRNA is stabilized and translation is promoted to enhance target gene expression. (See Table 1).
- the cis-element binding factors that inhibit the binding of polynucleotides and the like include AUF1, HuR, Hel-N1, HTP, TRF, BRF1, TIA-1, KSRP, GUG-BP2, Nucleotin, TINO, PAIP2, ZFP36L1 and It can be a protein such as ZFP36L2 or a miRNA such as miR16.
- ARE is estimated to be present in 5-8% of all genes including cytokines such as IL-2, IL-3, and NFTNF- ⁇ , transcription factors, and cell cycle-related proteins. By doing so, it is possible to control the expression of many genes that play an important role in maintaining homeostasis using the polynucleotides and the like according to the present invention.
- polynucleotide according to the present invention can be obtained by a known nucleic acid synthesis method.
- Polynucleotide analogs can also be obtained according to known methods described in Patent Documents 1 to 3, for example, when synthesized by LNA.
- Gene Expression Control Method when the control target is a cell, the polynucleotide is added to the cell culture medium and taken into the cell, so that It can be bound to the target cis element of the target gene mRNA.
- a polynucleotide or the like may be bound to the target cis element by introducing it into the cell by lipofection or microinjection.
- a polynucleotide or the like is produced by an administration route such as oral route, rectal route, nasal route, vascular route, or direct local administration to the target organ. It is introduced into the body or living organs and taken into cells.
- the polynucleotide can be expressed in the cell and bound to the target cis element of the target gene mRNA.
- plasmids derived from Escherichia coli, Bacillus subtilis or yeast, animal viruses such as bacteriophages, retroviruses, vaccinia viruses, baculoviruses, or those obtained by fusing them with liposomes can be used.
- the polynucleotide is RNA
- a retroviral vector or a fusion of these with a liposome is preferably selected.
- the expression vector can be constructed by a known genetic engineering technique.
- Target Gene Product Expression Enhancer or Suppressor and Pharmaceutical Composition Hereinafter, the target gene product expression enhancer or suppressor according to the present invention and a pharmaceutical composition containing this as an active ingredient will be described.
- the polynucleotide or the like according to the present invention can be used as an expression enhancer or suppressor of the target gene product.
- the gene product expression enhancer or suppressor comprises a polynucleotide and / or polynucleotide analog itself, or an expression vector capable of expressing the polynucleotide as an active ingredient.
- a method for treating an expression enhancer or suppressor on cells or living organs to be controlled is by adding the above-described polynucleotide or the like to a cell culture solution, introducing it into cells by lipofection, or oral administration, etc.
- An optimal method can be appropriately selected and used from methods such as introduction into living organs and expression in cells using an expression vector.
- this gene product expression enhancer or inhibitor is generally recognized together with a carrier, flavoring agent, excipient, vehicle, preservative, stabilizer, binder, etc. It can be produced by mixing in the unit dosage form required for the formulation.
- This pharmaceutical composition is sterilized, for example, orally as tablets, capsules, elixirs, microcapsules or the like with sugar coating as necessary, or with water or other pharmaceutically acceptable liquids. It can be used parenterally in the form of injections such as solutions or suspensions.
- additives that can mix the gene product expression enhancer or inhibitor according to the present invention into raw tablets, capsules and the like include binders such as gelatin, corn starch, tragacanth and gum arabic, crystalline cellulose Excipients such as corn starch, gelatin, alginic acid, etc., lubricants such as magnesium stearate, sweeteners such as sucrose, lactose or saccharin, flavors such as peppermint, red mono oil or cherry An agent is used.
- binders such as gelatin, corn starch, tragacanth and gum arabic
- crystalline cellulose Excipients such as corn starch, gelatin, alginic acid, etc.
- lubricants such as magnesium stearate
- sweeteners such as sucrose, lactose or saccharin
- flavors such as peppermint, red mono oil or cherry An agent is used.
- the dosage, dosage form, and administration method of the pharmaceutical composition are determined in consideration of the age, weight, symptoms, etc. of the administration subject (including animals).
- administration method administration by oral route, rectal route, nasal route, vascular route, or the like, or local administration to a target organ can be appropriately selected.
- LNA1-3, 6-14 polynucleotide analog having the nucleotide sequence shown in Table 2 as a low-density lipoprotein (LDLR). It was found that the expression of receptor) can be remarkably enhanced. LNA1 to 3, 6 to 14 bind to ARE1 (2790 to 2797 bases from the 5 ′ end of the LDLR mRNA base sequence shown in SEQ ID NO: 4) and function to promote destabilization and degradation of LDLR mRNA. It was revealed that the expression of LDLR mRNA was enhanced by inhibiting the binding of ZFP36L1 and ZFP36L2 to ARE, selectively stabilizing LDLR mRNA, and promoting translation.
- polynucleotides and polynucleotide analogs comprising the nucleotide sequence shown in any of SEQ ID NOs: 1 to 3 or 6 to 14, or a nucleotide sequence substantially identical to the nucleotide sequence, or the polynucleotide can be expressed
- the expression vector can be used as an LDLR expression enhancer.
- the “polynucleotide containing substantially the same base sequence” means one or more (preferably within a few) bases in the base sequence shown in any of SEQ ID NOs: 1 to 3 and 6 to 14.
- the “polynucleotide analog containing substantially the same base sequence” can bind to a base sequence complementary to these in the same manner as the base sequences shown in any of SEQ ID NOs: 1 to 3 and 6 to 14.
- a base sequence-like structure in which a compound capable of distinguishing and recognizing each base of adenine, guanine, cytosine and uracil is deleted one or more (preferably within several) compounds are deleted, added
- a polynucleotide analogue having a substituted or inserted modified sequence which can exhibit an LDLR expression enhancing effect equivalent to the polynucleotide analogue comprising the nucleotide sequence shown in any of SEQ ID NOs: 1 to 3 and 6 to 14 Means nucleotide analogues.
- the nucleotide sequence of a polynucleotide or the like completely matches the nucleotide sequence shown in any of SEQ ID NOs: 1 to 3 and 6 to 14, but it is not necessarily the same. It is not necessary, so long as it is a base sequence that can bind with high affinity to the target ARE of LDLR ⁇ ⁇ ⁇ ⁇ mRNA and form a double strand, the base sequence is substantially the same as the base sequence shown in SEQ ID NOs: 1 to 3 and 6 to 14. It only needs to contain an array.
- a polynucleotide or the like having such a modified sequence can be designed for the purpose of, for example, preventing side effects due to binding of other genes to mRNA, and improving cell membrane permeability and in vivo stability of polynucleotides and the like. .
- LDLR has a function of taking LDL cholesterol in plasma into cells by receptor-mediated endocytosis.
- intracellular uptake of LDL cholesterol by LDLR expressed in the liver plays an important role in the clearance of plasma LDL cholesterol.
- LDL cholesterol is responsible for cholesterol transport from the liver to peripheral tissues.
- LDL cholesterol causes vascular injury by depositing on the inner wall of the blood vessel, causing arteriosclerosis, hypertension, cerebral infarction, myocardial infarction, narrowing. causes heart disease.
- the LDLR expression enhancer according to the present invention can selectively enhance the expression of LDLR, according to the pharmaceutical composition containing this LDLR expression enhancer as an active ingredient, the LDLR expression enhancer is particularly applied to the liver. By enhancing the above, it becomes possible to increase the clearance of plasma LDL cholesterol and prevent, ameliorate or treat hyperlipidemia and hyperlipidemia-related diseases.
- the LDLR expression enhancer and pharmaceutical composition according to the present invention can be applied to prevent, ameliorate, or treat Alzheimer's disease by selectively enhancing the expression of LDLR and controlling the level of plasma LDL cholesterol. There is also a possibility.
- prevention includes not only pre-stage prevention of disease, but also prevention of recurrence after treatment of the disease.
- hypertension In addition to arteriosclerosis, hypertension, cerebral infarction, myocardial infarction, and angina, hyperlipidemia-related diseases include diabetes, obesity, and cancer. It is not excluded.
- LDLR expression control experiment I Using LDLR as a target gene, the polynucleotides and polynucleotide analogs according to the present invention and gene expression control methods using these were verified.
- FIG. 4 is a schematic diagram showing the positions of these three AREs in the 3′UTR of LDLR mRNA.
- the three AREs are formally expressed as “ARE1”, “ARE2”, and “ARE3” (note that “ARE1 to 3” are the three ARE groups “ARE I to III” described above. Is different).
- UCAU indicates “UCAU repeat” in which 4 bases of “UCAU” are arranged 4 times in duplicate.
- ARE1 to 3 are located in the region 2677-3585 bp from the 5 'end of 5175 bp of LDLR mRNA shown in SEQ ID NO: 4 (GenBank Accession No. NM_000527).
- ARE1 corresponds to a region from 2790 to 2797 bp from the 5 'end
- ARE2 corresponds to a region from 3350 to 3363 bp from the 5' end
- ARE3 corresponds to a region from 3538 to 3547 bp from the 5 'end.
- FIG. 5 shows the nucleotide sequence of a polynucleotide analog designed with ARE1 as the target cis element, and the partial nucleotide sequence of LDLR mRNA containing ARE1.
- FIG. 5 (A) shows the base sequence of the portion containing ARE1 (2767-2816 bps from the 5 ′ end) out of the total length of 5175 bps of LDLR mRNA (see SEQ ID NO: 4).
- Bold letters in the base sequence indicate ARE1, and the underlined portions indicate “AUUUA pentamer”.
- FIGS. 5 (B) to (D) show polynucleotide analogs having a base sequence of a polynucleotide analog complementary to a part of ARE1.
- bases indicated by capital letters indicate LNA
- bases indicated by small letters indicate RNA.
- the polynucleotide analogues shown in FIGS. 5 (B) to (D) are hybrid nucleic acids synthesized from LNA and RNA. Hereinafter, they will be referred to as LNA1 to LNA3, respectively.
- LNA1 to LNA3 were obtained by commissioned synthesis (Gene Design).
- the LNA1 shown in Fig. 5 (B) is a base sequence complementary to the entire ARE1 containing the "AUUUA pentamer" and a base sequence complementary to a part of the 5 ⁇ UTR and 3 ⁇ UTR continuous to the ARE1 of LDLR1 mRNA. (See SEQ ID NO: 1).
- the length of LNA1 is 11 bps, the 1st to 10th bases from the 5 ′ side are synthesized by LNA, and the 11th base is synthesized by RNA.
- the first base sequence from the 5 ′ side and the first to second base sequences from the 3 ′ side correspond to a base sequence specific to ARE1 of the LDLR gene (Gene-specific region: GSR). For GSR, see UTR described in Figure 2.
- the LNA2 shown in FIG. 5 (C) is composed of a base sequence complementary to the entire ARE1 containing the “AUUUA pentamer” and a base sequence complementary to a part of the 3 ′ UTR continuous to the ARE1 of LDLR mRNA ( (See SEQ ID NO: 2).
- the length of LNA2 is 19 bps, the 1st to 8th and 17th to 18th bases from the 5 ′ side are synthesized from LNA, and the 9th to 16th and 19th bases are synthesized from RNA.
- the first to ninth base sequences from the 5 ′ side correspond to the GSR of ARE1 of the LDLR gene.
- LNA3 shown in FIG. 5 (D) is composed of a base sequence complementary to a part of ARE1 and a base sequence complementary to a part of 3 ′ UTR continuous to ARE1 of LDLR mRNA (see SEQ ID NO: 3). .
- the length of LNA3 is 11 bps, the 1st to 10th bases from the 5 ′ side are synthesized by LNA, and the 11th base is synthesized by RNA.
- the first to fifth base sequences from the 5 ′ side correspond to the GSR of ARE1 of the LDLR gene.
- the mixture was allowed to stand at room temperature for 5 minutes, and the renucleotide analog diluent and Lipofectamine diluent were mixed. After further standing for 20 minutes, the entire amount was added to each well of a 6-well plate.
- LNA4 and LNA5 shown in Table 3 below were used as controls in addition to the above LNA1 to LNA3. These are polynucleotide analogues having a base sequence that does not show any complementarity to LDL mRNA.
- bases indicated by capital letters indicate LNA
- bases indicated by small letters indicate RNA.
- FIG. 6 The results of Western blotting are shown in “FIG. 6”.
- the upper part of Fig. (A) shows the detection band of LDLR, and the lower part shows the detection band of ⁇ -actin for comparison.
- an antibody Cat. No. 4970 manufactured by Cell Signaling was used.
- FIG. (B) the quantitative value of the detection band concentration is shown by a relative ratio where 1 is the cell in which transfection was not performed (lane 1 in FIG. (A)).
- LNA1 Since LNA1 has a short base sequence corresponding to the GSR of the LDLR gene, it can also bind to AREs of other gene mRNAs having the same base sequence as ARE1. For this reason, it is considered that LNA1 has a small amount of polynucleotide analog selectively bound to LDLR mRNA and has a smaller LDLR expression enhancing effect than LNA2 and LNA3.
- the base sequence corresponding to GSR of ARE1 of LDLR gene is made the 1st to 9th base sequence from the 5 ′ side, so that it is specific to ARE1 of LDL mRNA based on its sequence specificity. Can be combined. For this reason, it is considered that LNA2 has enhanced gene expression selectively with LDLR, and has a higher effect than LNA1.
- LNA2 synthesizes the base sequence complementary to the 10th to 16th ARE1 from the 5 ′ side with RNA
- LNA3 synthesizes the entire length with LNA (except for the 3 ′ end). ).
- LNA3 exhibits higher nuclease resistance than LNA2.
- the base sequence complementary to ARE1 of LNA3 synthesized by LNA has higher binding ability to ARE1 and excellent sequence specificity than the same sequence of LNA2 synthesized by RNA. For this reason, it is considered that LNA3 bound to ARE1 more efficiently and firmly than LNA2, and exhibited a high LDLR expression enhancing effect.
- LNA 1 to 3 can specifically enhance the expression level of LDLR as a target gene.
- Example 2 Identification of ARE binding factor
- LNA1-3 specifically binds to ARE1 of LDLR gene and inhibits binding of ARE binding factor to ARE1, and has a negative expression control function of ARE binding factor. By inhibiting, it was suggested that the expression level of LDLR was selectively enhanced. Therefore, we next identified an ARE-binding factor that inhibits binding to ARE1 by LNA1-3, and tried to analyze its function.
- LDL mRNA bait LDL mRNA was synthesized by in vitro translation. Amplification of the 2677-3585bp region of LDLR mRNA (SEQ ID NO: 4, GenBank Accession No. NM_000527) by PCR using a primer having a T7 promoter sequence at the 5 'end, and MEGAscript T7 kit (Cat. No. 1333, Ambion) was used to synthesize RNA according to the attached protocol. A reaction for covalently binding Flag-hydrazide to the 3 ′ end of the synthesized LDL mRNA was performed, and the 3 ′ end of LDLR mRNA was labeled with Flag.
- the labeled mRNA was purified using the Qiagen RNeasy Mini Kit (Cat. No. 74106).
- the mRNA is labeled with a flag according to a known method (see “Programmable ribozymes for mischarging tRNA with nonnatural amino acids and their applications to translation.” Methods, 2005, Vol, 36, No. 3, p.239-244). It was.
- the eluate sample was treated with lysyl endopeptidase, and the LC-MS / MS method (“A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics.” Analytical Chemistry, 2002, Vol. .74, No. 18, p. 4725-4733).
- QSTAR XL (Applied Biosystem) was used for the mass spectrometer.
- ZFP36L1 and ZFP36L2 were identified by LC-MS / MS.
- ZFP36L1 and ZFP36L2 form one family of ARE binding factors (hereinafter referred to as “ZFP36 family”) together with ZFP36 (also known as TTP).
- ZFP36 family has been reported to bind to ARE and destabilize mRNA and function to promote degradation (“Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly (A) ribonuclease. ”Molecular Cell Biology, 2003, Vol. 23, No. 11, p. 3798-812). Therefore, it was strongly suggested that the ARE binding factors having the negative expression control function assumed in Example 1 and capable of inhibiting the binding to ARE1 by LNA1-3 are ZFP36L1 and ZFP36L2.
- Example 3 Evaluation of binding ability of ZFP36L1 and ZFP36L2 to LDLR mRNA and inhibition of binding by LNA1-3 ZFP36L1 and ZFP36L2 can bind to 3 ⁇ UTR of LDLR mRNA, and LNA1 ⁇ 3 can bind to 3 ⁇ UTR of this ZFP family We examined whether it could be inhibited.
- Example 2 according to the method described in “(1) Synthesis of LDLR mRNA bait”, 3′UTR of LDLR mRNA containing ARE1 was synthesized by in vitro translation and labeled with Flag peptide.
- 3 ′ UTR 2677-3585 bps from the 5 ′ end of LDLR mRNA (see SEQ ID NO: 4) was used.
- synthesis was also performed in the same manner for the 3 ′ UTR (1199-1770 bps from the 5 ′ end) of beta-Actin® mRNA (SEQ ID NO: 5, GenBank Accession No. NM_001101).
- LDLR 3′UTR-Flag Flag-labeled LDLR 3′UTR
- Actin 3′UTR Actin 3′UTR
- -Flag cell extract protein extracted from 293T cells in which ZFP36L1 and ZFP36L2 (Myc-tagged-ZFP36L1 / ZFP36L2) fused with Myc protein were forcibly expressed, and LNA1-5 (Fig. 3 and Table 2)
- the reaction was carried out at a final concentration of 0, 30 and 100 ⁇ M.
- the eluted sample was subjected to Western blotting using an anti-Myc antibody (Cat. No. 1667149, Roche).
- FIG. 7 Western blot results are shown in “Fig. 7”. The upper row shows the Myc-tagged-ZFP36L2 band for anti-Myc antibody detection, and the lower row shows the Myc-tagged-ZFP36L1 band.
- ZFP36L1 and a large amount of ZFP36L2 were detected in a sample obtained by mixing cell extract protein containing Myc-tagged-ZFP36L1 / ZFP36L2 with LDLR 3 ⁇ UTR-Flag and immunoprecipitating with anti-Flag antibody (lane 3 in the figure) It was done.
- the detection signal of ZFP36L1 and ZFP36L2 was extremely low in the sample (lane 2) obtained by the reaction with Actin 3′UTR-Flag.
- Myc-tagged-ZFP36L1 / ZFP36L2 can bind to LDLR 3 ⁇ UTR-Flag, and that ZFP36L1 and ZFP36L2 have the ability to bind LDLR to 3 ⁇ UTR.
- Lanes 8-13 where cell extracts and LDLR 3 ⁇ UTR-Flag were reacted in the presence of LNA1 ⁇ 3 containing a nucleotide sequence complementary to ARE1 of LDLR mRNA, the detection signals of ZFP36L1 and ZFP36L2 were significantly Declined.
- the intensity of the detection band in lanes 8-13 was less than or equal to the intensity of the detection band obtained by the reaction with Actin 3′UTR-Flag shown in lane 2.
- Lanes 4-7 where the reaction was carried out with the addition of control LNA4, 5, no decrease in the detection signals of ZFP36L1 and ZFP36L2 was observed.
- Hela cells were seeded on a 6-well plate at 4.0 ⁇ 10 5 cells / well, and cultured using 10% FBS-containing DMEM medium. After 24 hours of culture, siRNA was transfected into the cells by lipofection (Lipofectamine 2000, Cat. No. 11668-019, Invitrogen). 200 ⁇ mol of each polynucleotide analogue was diluted with 100 ⁇ l with Opti-MEM. Further, 10 ⁇ l of Lipofectamine 2000 was diluted with 100 ⁇ l of Opti-MEM.
- the mixture was allowed to stand at room temperature for 5 minutes, the renucleotide analog dilution solution and the Lipofectamine dilution solution were mixed, and after further standing for 20 minutes, the entire amount was added to each well of a 6-well plate.
- siRNA used was purchased from Invitrogen. For each of ZFP36L1 and ZFP36L2, the Cat.No. of the three types of siRNA used is shown in “Table 4”. Well, Stealth RNAi Negative Control (Cat. No. 12935-100, Invitrogen) was used as the control siRNA 7-9.
- FIG. 8 Western blot results are shown in “Fig. 8”.
- the upper part of Fig. (A) shows the detection band of LDLR, and the lower part shows the detection band of ⁇ -actin for comparison.
- FIG. (B) the quantitative value of the detection band concentration is shown as a relative ratio with 1 being the cell transfected with control siRNA7 (lane 1 in FIG. (A)).
- LDLR expression level is significantly higher in cells transfected with any of siRNA 1-3 and siRNA 4-6 in combination and suppressed ZFP36L1 and ZFP36L2 expression Enhanced.
- LDLR low-density lipoprotein
- LNA1 to 3 bind to ARE1 of LDLR mRNA, inhibit the binding of ZFP36L1 and ZFP36L2 to ARE1, and bind to ARE1 to bind ZFP36L1 and ZFP36L2. It was shown that the expression of LDLR mRNA is selectively enhanced by inhibiting the negative gene expression control function, stabilizing LDLR mRNA and promoting translation (see Fig. 9).
- LDLR expression control experiment II (ARE2 and ARE3) Next, among the three AREs present in the 3'UTR of LDLR mRNA (see Fig. 4), ARE2 and ARE3 were used as target cis-elements, and the same LDLR as in Example 1 Expression control experiments were performed.
- LNA26 has a base sequence complementary to ARE2 (3350 to 3363 bps from the 5 ′ end) out of the total length of 5175 bps of LDLR mRNA (see SEQ ID NO: 4).
- LNA27 has a base sequence complementary to ARE3 (3538-3547 bps from the 5 ′ end).
- Example 6 In the cells transfected with LNA3 used in Example 1 (lane 2), a marked increase in LDLR expression was confirmed as compared to the cells not transfected with the polynucleotide analogue (lane 1). . In contrast, no increase in LDLR expression was observed in cells transfected with LNA26 and LNA27 (lanes 3 and 4). For ⁇ -actin, no significant change in the expression level was observed in all cells regardless of the presence or absence of transfection and the type of LNA transfected. ⁇ Example 6> 6). Evaluation of binding ability of ZFP36L1 and ZFP36L2 to ARE2 or ARE3 In this example, it was examined whether the ZFP family can bind to ARE2 or ARE3.
- FIG. 11 shows the structure of the synthesized mRNA bait.
- FIG. 11 (A) is an mRNA bait (LDLR 3′UTR- (WT) -Flag) containing the 2677-3585 bp region of LDLR mRNA).
- B) and (C) are the mRNA bait (LDLR 3'UTR- ( ⁇ -ARE1) -Flag) from which the ARE1 portion has been deleted and the mRNA bait (LDLR 3'UTR from which ARE2 and ARE3 have been deleted, respectively. -( ⁇ -ARE2,3) -Flag).
- Each mRNA bait was synthesized according to the method described in “(1) Synthesis of LDLR mRNA bait” in Example 2.
- ZFP36L1 and ZFP36L2 were hardly detected in the sample (lane 4) obtained by the reaction with LDLRlag3′UTR- ( ⁇ -ARE1) -Flag from which ARE1 was deleted. This indicates that ZFP36L1 and ZFP36L2 bind specifically to ARE1 and not ARE2 and ARE3.
- LDLR 3'UTR- ( ⁇ -ARE2,3) -Flag lacking ARE2 and ARE3 is mixed with cell extract protein containing Myc-tagged-ZFP36L1 / ZFP36L2, and immunoprecipitated with anti-Flag antibody.
- LDLR 3′UTR- (WT) -Flag including the entire region was detected. This supports that ZFP36L1 and ZFP36L2 are bound to ARE1.
- LDLR expression control experiment III (ARE1)
- ARE1 LDLR expression control experiment III
- Table 6 shows the nucleotide sequences of the newly designed polynucleotide analogues (LNA 6-25). In the base sequence, the underlined portion indicates a base sequence complementary to ARE1 (see also FIG. 5).
- Flag-labeled LDLR 3′UTR was mixed with cell-extracted protein extracted from 293T cells in which ZFP36L2 (Myc-tagged- ZFP36L2) fused with Myc protein was forcibly expressed, and LNA3 and LNA6 The reaction was carried out with addition of ⁇ 25. After immunoprecipitation, the eluted sample was subjected to Western blotting using an anti-Myc antibody. The results of Western blotting are shown in “FIG. 14”.
- ZFP36L2 A large amount of ZFP36L2 was detected in a sample obtained by mixing cell extract protein containing Myc-tagged-ZFP36L2 with LDLR 3'UTR-Flag and immunoprecipitating with anti-Flag antibody ("Blank" in the figure).
- the detection signal of ZFP36L2 decreased significantly in the sample prepared by reacting cell extract protein with LDLR 3 ⁇ UTR-Flag in the presence of LNA6-14, which showed the enhancement effect of LDLR expression level. . This confirmed that LNA6-14 inhibited the binding of ZFP36L2 to ARE1.
- the expression of LDLR could be positively controlled using a polynucleotide analog having a base sequence complementary to a specific ARE on LDLR mRNA, such as the polynucleotide according to the present invention. It shows the possibility that the same gene expression control can be performed for various genes by appropriately setting the base sequence according to the target gene and the target cis element.
- the expression of LDLR is designed by inhibiting the function of cis-element binding factors other than ZFP36L1 and ZFP36L2 by designing polynucleotides that can bind to AREs other than ARE1 to ARE3 or cis-elements other than ARE. It is thought that it can be controlled not only positively but also negatively. Therefore, according to the gene expression control method according to the present invention, it was considered that the expression of various genes can be controlled positively and negatively.
- Example 1 it is a figure which shows the base sequence of the polynucleotide analog LNA1-3 which designed ARE1 as a target cis element, and the partial base sequence of LDLR (TM) mRNA containing ARE1.
- TM LDLR
- Example 1 it is a figure which shows the result of having evaluated the expression level of LDLR by Western blot.
- A Upper row shows LDLR detection band, lower row shows ⁇ -actin detection band.
- (B) shows the quantitative value of the detected band concentration as a relative ratio.
- Example 3 it is a figure which shows the result of having evaluated the binding ability of ZFP36L1 and ZFP36L2 to LDLR mRNA 3'UTR and the binding inhibition ability by LNA1-3 by Western blot.
- Example 4 it is a figure which shows the result of the Western blot which examined the change of the LDLR expression level at the time of suppressing the expression of ZFP36L1 and ZFP36L2 using RNAi.
- A Upper row shows LDLR detection band, lower row shows ⁇ -actin detection band.
- B shows the quantitative value of the detected band concentration as a relative ratio. It is a schematic diagram explaining the expression enhancement mechanism of LDLR by the polynucleotide etc. which concern on this invention.
- Example 5 it is a figure which shows the result of having evaluated the expression level of LDLR by Western blot.
- A Upper row shows LDLR detection band, lower row shows ⁇ -actin detection band.
- Example 6 it is a schematic diagram which shows the structure of mRNA bait synthesize
- Example 6 it is a figure which shows the result of having evaluated the binding ability of ZFP36L1 and ZFP36L2 to ARE1-3 by Western blot.
- Example 7 it is a figure which shows the result of having evaluated the expression level of LDLR by Western blot.
- Example 7 it is a figure which shows the result of having evaluated the binding inhibitory ability with respect to ZFP36L2 of LNA6-25 by Western blot.
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Abstract
Description
このポリヌクレオチド又はポリヌクレオチド類似体は、前記シスエレメントへのシスエレメント結合因子の結合を阻害することにより、前記mRNAを選択的に安定化して遺伝子産物の翻訳を促進、又は不安定化して遺伝子産物の翻訳を阻害する。
ポリヌクレオチド又はポリヌクレオチド類似体が結合阻害の対象とするシスエレメント及びシスエレメント結合因子は、特にAUリッチエレメント及びAUリッチエレメント結合因子とすることができる。
本発明において、上記のポリヌクレオチド類似体は、特にLNAを含んでなることが好適である。
併せて、本発明は、標的遺伝子産物発現増強剤又は抑制剤製造のための上記ポリヌクレオチド及び/又はポリヌクレオチド類似体、若しくは該ポリヌクレオチドを発現可能な発現ベクターの使用と、特にポリヌクレオチドを発現可能な発現ベクターをも提供する。
このポリヌクレオチド及びポリヌクレオチド類似体は、ARE1(配列番号4に示すLDLR mRNA塩基配列の5´末端側から2790~2797塩基)へのシスエレメント結合因子の結合を阻害するものである。
このポリヌクレオチド及びポリヌクレオチド類似体は、配列番号1~3、6~14のいずれかに示す塩基配列、又は該塩基配列と実質的に同一の塩基配列、を含むものとすることができる。
併せて、本発明は、上記ポリヌクレオチドを発現可能な発現ベクターをも提供する。
図1は、シスエレメント及びシスエレメント結合因子によるmRNAの安定性及びタンパクへの翻訳量の制御メカニズムを説明する模式図である。
本発明に係るポリヌクレオチド及びポリヌクレオチド類似体は、mRNAのシスエレメントに相補的な塩基配列を有し、シスエレメントの少なくとも一部に結合することによって、このシスエレメント結合因子による正負の発現制御機能を阻害するものである。以下、本発明に係るポリヌクレオチド及びポリヌクレオチド類似体の構成と機能について具体的に説明する。
本発明に係るポリヌクレオチド等を、ポリヌクレオチド類似体とする場合、cCIS及びcUTRは、シスエレメント及び3´側UTRの塩基配列中のアデニン、グアニン、シトシン及びウラシルの各塩基を区別して認識し、相補的に結合し得る化合物が配列させた塩基配列様構造を有する領域とすることができることは既に述べた通りである。加えて、本発明に係るポリヌクレオチド類似体は、ポリヌクレオチドにおいてはリボースのフォスフォジエステル結合により形成されている基本分子鎖を、リボースを改変した基本分子鎖や、リボース以外の糖を結合させた基本分子鎖、またこれらがフォスフォジエステル結合以外の化学結合により結合させた基本分子鎖とすることができる。
本発明に係るポリヌクレオチド等が対象とするターゲットシスエレメントは、例えばAUリッチエレメント、Histone mRNA 3´ UTR stem loop element、Internal ribosome entry site (IRES)、A2RE element、ZIPCODE element、Iron response element(IRE)、Cytoplasmic polyadenylation (CPE)、Nanos translational control、Amyloid precursor protein element (APP)、Translational regulation element (TGE)/direct repeat element (DRE)、Bruno element (BRE)、15-lipoxygenase differentiation control element (15-LOX-DICE)、G-quartet element、Adh mRNA down-regulation element、Barley yellow dwarf virus、GLUT1 mRNA-stability control element、Msl-2 3 UTR control element、Msl-2 5 UTR control element、Ribosomal S12 mRNA translational control element、Selenocysteine insertion sequence type 1 (SECIS-1)、Selenocysteine insertion sequence type 2 (SECIS-2)、TNF-mRNA stability control element、Terminal oligopyrimidine tract (TOP)、 Vimentin mRNA 3 UTR control elementなどとすることができる。
本発明に係るポリヌクレオチドは公知の核酸合成方法によって得ることができる。また、ポリヌクレオチド類似体についても、例えばLNAにより合成する場合には、上記特許文献1~3に記載された公知の方法に従って得ることが可能である。
本発明に係るポリヌクレオチド等を用いた遺伝子発現制御方法において、制御対象を細胞とする場合、ポリヌクレオチド等は、細胞培養液に添加し細胞内へ取り込ませることによって、細胞内の標的遺伝子mRNAのターゲットシスエレメントへ結合させることができる。また、ポリヌクレオチド等をリポフェクションやマイクロインジェクションによって細胞内へ導入することにより、ターゲットシスエレメントへ結合させてもよい。また、遺伝子発現の制御対象が生体臓器又は生体である場合には、経口経路、直腸経路、鼻腔経路、血管経路などの投与経路によって、又は対象臓器への直接局所投与によって、ポリヌクレオチド等を生体内又は生体臓器内へ導入し、細胞内へ取り込ませる。
以下、本発明に係る標的遺伝子産物の発現増強剤又は抑制剤、及びこれを有効成分として含有する医薬組成物について説明する。
本発明者らは、実施例において詳しく後述するように、「表2」に示す塩基配列を有するポリヌクレオチド類似体(LNA1~3, 6~14)が、LDLR(low- density lipoprotein receptor)の発現を顕著に増強し得ることを見出した。そして、LNA1~3, 6~14が、ARE1(配列番号4に示すLDLR mRNA塩基配列の5´末端側から2790~2797塩基)に結合して、LDLR mRNAの不安定化・分解促進に機能するZFP36L1及びZFP36L2のAREへの結合を阻害し、LDLR mRNAを選択的に安定化して翻訳を促進することにより、LDLR mRNAの発現を増強させていることを明らかにした。
1.LDLRの発現制御実験I(ARE1)
LDLRを標的遺伝子として、本発明に係るポリヌクレオチド及びポリヌクレオチド類似体、並びにこれらを用いた遺伝子発現制御方法について検証を行った。
AREのデータベース(AU-RICH ELEMENT-CONTAINING mRNA DATABASE :http://brp.kfshrc.edu.sa/ARED/)を参照し、LDLR mRNA上のAREの一部に相補的なポリヌクレオチド類似体を設計した。
Hela細胞を、6-well プレートに4.0×105 cells / wellで播種し、10%FBS 含有DMEM培地を用いて培養した。培養24時間後、ポリヌクレオチド類似体をリポフェクション(Lipofectamine 2000, Cat.No.11668-019, Invitrogen)によって細胞にトランスフェクトした。ポリヌクレオチド類似体それぞれ200pmolを100μlのOpti-MEMで希釈した。また、10μlのLipofectamine2000を100μlのOpti-MEMで希釈した。希釈後室温にて5分間静置し、リヌクレオチド類似体希釈液とLipofectamine希釈液を混合し、さらに20分静置後、全量を6-well プレートの各ウェルに加えた。
トランスフェクション24時間後の細胞を回収し、定法によりタンパク抽出を行った。抽出したタンパク5μgをSDS-PAGEにより分離し、メンブレンにブロットした。定法によりウェスタンブロットを行い、LDLRの発現量を評価した。抗LDLR抗体には、Abcam社の抗体(Cat.No.ab52818)を使用した。
ウェスタンブロットの結果を「図6」に示す。図(A)上段にはLDLRの検出バンド、下段には比較のためβ-actinの検出バンドを示す。なお、抗β-actinには、Cell Signaling社の抗体(Cat.No.4970)を用いた。また、図(B)には、検出バンド濃度の定量値を、トランスフェクションを行わなかった細胞(図(A)中レーン1)を1とした相対比によって示す。
2.ARE結合因子の同定
実施例1の結果により、LNA1~3が、LDLR遺伝子のARE1に特異的に結合してARE結合因子のARE1への結合を阻害し、ARE結合因子の負の発現制御機能を阻害することで、LDLRの発現量を選択的に増強していることが示唆された。そこで、次に、LNA1~3によってARE1への結合を阻害されるARE結合因子を同定し、その機能を解析することを試みた。初めに、ARE1に結合するARE結合因子を同定することを目的として、LDL mRNA をベイトとした免疫沈降とマススペクトロメーターを用いたプロテオーム解析により、LDL mRNAのシスエレメントに結合するシスエレメント結合タンパクについて網羅的解析を行った。
LDL mRNAをin vitro translationにより合成した。5´末端にT7 promoter 配列を持つプライマーを用いてPCRによりLDLR mRNA (配列番号4、GenBank Accession No.NM_000527)の2677-3585bp領域の増幅を行い、MEGAscript T7 キット(Cat.No.1333, Ambion)を用い、添付プロトコールに従ってRNAの合成を行った。合成されたLDL mRNAの3´末端にFlag-hydrazideを共有結合させる反応を行い、LDLR mRNAの3´末端をFlag標識した。標識mRNAをQiagen社のRNeasy Mini Kit(Cat.No.74106)を用いて精製した。なお、mRNAのFlag標識は、公知の手法(”Programmable ribozymes for mischarging tRNA with nonnatural amino acids and their applications to translation.” Methods, 2005, Vol,36, No.3, p.239-244参照)に従って行った。
精製後のFlag標識LDL mRNA 10pmolを抗Flag抗体ビーズ(Cat.No.F2426, Sigma)と混合、4℃で1時間反応を行った。その後、10%FBS 含有DMEM培地で培養した293T細胞から抽出した細胞抽出タンパク3mgを加えさらに、4℃で1時間反応を行った。非結合タンパク質を洗い流した後、RNA及びRNA結合タンパクをFlagペプチドで溶出させた。溶出させて得た試料を、リジルエンドペプチダーゼ処理し、公知の手法であるLC-MS/MS法を用い(”A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics.” Analytical Chemistry, 2002, Vol.74, No.18, p.4725-4733参照)に従って、解析を行った。マススペクトロメーターには、QSTAR XL(アプライドバイオシステム)を用いた。
LC-MS/MSにより「ZFP36L1」及び「ZFP36L2」が同定された。ZFP36L1及びZFP36L2は、ZFP36(別名TTP)とともにARE結合因子の1ファミリー(以下、「ZFP36ファミリー」という)を形成している。ZFP36ファミリーは、AREに結合してmRNAを不安定化し、分解促進に機能していることが報告されている(”Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease.” Molecular Cell Biology, 2003, Vol.23, No.11, p.3798-812参照)。従って、実施例1で想定された負の発現制御機能を有し、LNA1~3によってARE1への結合を阻害され得るARE結合因子が、ZFP36L1及びZFP36L2である可能性が強く示唆された。
3.ZFP36L1及びZFP36L2のLDLR mRNAへの結合能及びLNA1~3による結合阻害能評価
ZFP36L1及びZFP36L2がLDLR mRNAの3´UTR に結合し得るか、またLNA1~3がこのZFPファミリーの3´UTRへの結合を阻害し得るかについて検討を行った。
4.ZFP36L1及びZFP36L2の機能解析 ZFP36L1及びZFP36L2のLDLR発現制御における機能を明らかにするため、RNAiを用いてZFP36L1及びZFP36L2の発現を抑制した場合のLDLR発現量の変化について検討を行った。
5.LDLRの発現制御実験II(ARE2・ARE3) 次に、LDLR mRNAの3’UTRに存在する3つのARE(図4参照)のうち、ARE2及びARE3をターゲットシスエレメントとして、実施例1と同様のLDLR発現制御実験を行った。
「表5」に、ARE2又はARE3をターゲットシスエレメントとして設計したポリヌクレオチド類似体の塩基配列を示す。LNA26は、LDLR mRNA(配列番号4参照)の全長5175bpsうち、ARE2(5´側末端から3350~3363bps)に相補的な塩基配列を有する。また、LNA27は、ARE3(5´側末端から3538-3547bps)に相補的な塩基配列を有する。
ウェスタンブロットの結果を「図10」に示す。図中、上段にはLDLRの検出バンド、下段には比較のためβ-actinの検出バンドを示す。
<実施例6>
6.ZFP36L1及びZFP36L2のARE2又はARE3への結合能評価
本実施例では、ARE2又はARE3に対してZFPファミリーが結合し得るかについて検討を行った。
ARE1~3を欠失させたベイトを合成し、ZFP36L1及びZFP36L2のARE1~3への結合能を評価した。「図11」に合成したmRNAベイトの構造を示す。図11(A)は、LDLR mRNAの2677-3585bp領域を含むmRNAベイトLDLR 3’UTR-(WT)-Flag)である。また(B)及び(C)は、それぞれARE1部分を欠失させたmRNAベイト(LDLR 3’UTR-(Δ-ARE1)-Flag)とARE2及びARE3を欠失させたmRNAベイト(LDLR 3’UTR-(Δ-ARE2,3)-Flag)である。各mRNAベイトの合成は、実施例2中「(1)LDLR mRNAベイトの合成」で説明した方法に従って行った。
実施例3で説明した方法に従い、精製後のFlag標識mRNAを抗Flag抗体ビーズと混合・反応を行った後、Myc-tagged-ZFP36L1/ ZFP36L2を強制発現させた293T細胞から抽出した細胞抽出タンパク質3mgと混合し反応を行なった。免疫沈降後、溶出させた試料について、抗Myc抗体(Cat.No.1667149, Roche)を用いてウエスタンブロッティングを行った。
ウェスタンブロットの結果を「図12」に示す。上段は抗Myc抗体検出のMyc-tagged-ZFP36L2バンド、下段はMyc-tagged-ZFP36L1バンドを示す。
7. LDLRの発現制御実験III(ARE1)
本実施例では、ARE1をターゲットシスエレメントとして数種類のポリヌクレオチド類似体を設計し、LDLRの発現増強(図9参照)を試みた。
Claims (17)
- mRNAのシスエレメントの少なくとも一部に相補的な塩基配列と、該シスエレメントの5´側及び/又は3´側の非翻訳領域の一部に相補的な塩基配列と、を有し、
前記非翻訳領域に相補的な塩基配列が有する配列特異性に基づいて、前記シスエレメントの少なくとも一部に特異的に結合することにより、
該シスエレメントへのシスエレメント結合因子の結合を阻害し得ることを特徴とするポリヌクレオチド又はポリヌクレオチド類似体。 - 前記シスエレメントへのシスエレメント結合因子の結合を阻害することにより、
前記mRNAを選択的に安定化して遺伝子産物の翻訳を促進すること、又は不安定化して遺伝子産物の翻訳を阻害する請求の範囲第1項記載のポリヌクレオチド又はポリヌクレオチド類似体。 - AUリッチエレメントへのAUリッチエレメント結合因子の結合を阻害する請求の範囲第2項記載のポリヌクレオチド又はポリヌクレオチド類似体。
- 前記mRNAがLDLR mRNAであって、前記AUリッチエレメントへのZFP36L1及び/又はZFP36L2の結合を阻害する請求の範囲第3項記載のポリヌクレオチド又はポリヌクレオチド類似体。
- 前記mRNAがLDLR mRNAであって、ARE1(配列番号4に示すLDLR mRNA塩基配列の5´末端側から2790~2797塩基)へのシスエレメント結合因子の結合を阻害する請求の範囲第3項又は第4項記載のポリヌクレオチド又はポリヌクレオチド類似体。
- 配列番号1~3、6~14のいずれかに示す塩基配列、又は該塩基配列と実質的に同一の塩基配列、を含むことを特徴とする請求の範囲第4項又は第5項記載のポリヌクレオチド及びポリヌクレオチド類似体。
- 請求の範囲第4項~第6項記載のいずれか一以上のポリヌクレオチド及び/又はポリヌクレオチド類似体を含有する、若しくはいずれか一以上の該ポリヌクレオチドを発現可能な発現ベクターを含有するLDLR発現増強剤。
- 請求の範囲第7項記載のLDLR発現増強剤を有効成分として含有する、高脂血症あるいは高脂血症関連疾患を予防、改善又は治療するための医薬組成物。
- 前記疾患が、動脈硬化症、高血圧症、脳梗塞、心筋梗塞、狭心症、糖尿病、肥満症、癌からなる群より選択される一以上の疾患である請求の範囲第8項記載の医薬組成物。
- 請求の範囲第7項記載のLDLR発現増強剤を有効成分として含有する、アルツハイマー病を予防、改善又は治療するための医薬組成物。
- 請求の範囲第6項記載のポリヌクレオチドを発現可能な発現ベクター。
- 請求の範囲第1項記載のポリヌクレオチド及び/又はポリヌクレオチド類似体を含有する、若しくは該ポリヌクレオチドを発現可能な発現ベクターを含有する遺伝子産物発現増強剤又は抑制剤。
- 請求の範囲第12項記載の遺伝子産物発現増強剤又は抑制剤を有効成分として含有する医薬組成物。
- 遺伝子産物発現増強剤又は抑制剤製造のための請求の範囲第1項記載のポリヌクレオチド又はポリヌクレオチド類似体、若しくは該ポリヌクレオチドを発現可能な発現ベクターの使用。
- 請求の範囲第1項記載のポリヌクレオチドを発現可能な発現ベクター。
- LNAを含んでなることを特徴とする請求の範囲第1項記載のポリヌクレオチド類似体。
- mRNAのシスエレメントの少なくとも一部に相補的な塩基配列と、該シスエレメントの5´側及び/又は3´側の非翻訳領域の一部に相補的な塩基配列と、を有するポリヌクレオチド及び/又はポリヌクレオチド類似体を用い、
前記シスエレメントへのシスエレメント結合因子の結合を阻害することにより、
標的遺伝子産物の発現を制御することを特徴とする遺伝子発現制御方法。
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US (1) | US20100297750A1 (ja) |
EP (1) | EP2246422A4 (ja) |
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WO2011010583A1 (ja) * | 2009-07-22 | 2011-01-27 | 株式会社Galaxy Pharma | オリゴヌクレオチドのスクリーニング方法及びオリゴヌクレオチドライブラリー |
JP2016528897A (ja) * | 2013-08-16 | 2016-09-23 | ラナ セラピューティクス インコーポレイテッド | Rnaを調節するための組成物および方法 |
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- 2008-12-04 WO PCT/JP2008/072088 patent/WO2009093384A1/ja active Application Filing
- 2008-12-04 JP JP2009550433A patent/JPWO2009093384A1/ja active Pending
- 2008-12-04 EP EP08871274A patent/EP2246422A4/en not_active Withdrawn
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JPH10195098A (ja) | 1996-11-18 | 1998-07-28 | Takeshi Imanishi | 新規ヌクレオチド類縁体 |
WO1998039352A1 (fr) * | 1997-03-07 | 1998-09-11 | Takeshi Imanishi | Nouveaux analogues de bicyclonucleoside et d'oligonucleotide |
JPH10304889A (ja) | 1997-03-07 | 1998-11-17 | Takeshi Imanishi | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
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WO2011010583A1 (ja) * | 2009-07-22 | 2011-01-27 | 株式会社Galaxy Pharma | オリゴヌクレオチドのスクリーニング方法及びオリゴヌクレオチドライブラリー |
JP2016528897A (ja) * | 2013-08-16 | 2016-09-23 | ラナ セラピューティクス インコーポレイテッド | Rnaを調節するための組成物および方法 |
US10758558B2 (en) | 2015-02-13 | 2020-09-01 | Translate Bio Ma, Inc. | Hybrid oligonucleotides and uses thereof |
Also Published As
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EP2246422A4 (en) | 2012-07-25 |
JPWO2009093384A1 (ja) | 2011-05-26 |
US20100297750A1 (en) | 2010-11-25 |
EP2246422A1 (en) | 2010-11-03 |
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