WO2009054136A1 - 融合ポリペプチド結合磁気微粒子による細胞分離方法 - Google Patents

融合ポリペプチド結合磁気微粒子による細胞分離方法 Download PDF

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Publication number
WO2009054136A1
WO2009054136A1 PCT/JP2008/003007 JP2008003007W WO2009054136A1 WO 2009054136 A1 WO2009054136 A1 WO 2009054136A1 JP 2008003007 W JP2008003007 W JP 2008003007W WO 2009054136 A1 WO2009054136 A1 WO 2009054136A1
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WO
WIPO (PCT)
Prior art keywords
polypeptide
magnetic microparticle
antibody
target cell
amino acid
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PCT/JP2008/003007
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English (en)
French (fr)
Inventor
Tadashi Matsunaga
Haruko Takeyama
Tomoko Yoshino
Masayuki Takahashi
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Tokyo University Of Agriculture And Technology Tlo Co., Ltd.
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Application filed by Tokyo University Of Agriculture And Technology Tlo Co., Ltd. filed Critical Tokyo University Of Agriculture And Technology Tlo Co., Ltd.
Publication of WO2009054136A1 publication Critical patent/WO2009054136A1/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0006—Modification of the membrane of cells, e.g. cell decoration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　簡便で細胞生存に対する悪影響のない、高収率且つ高純度で細胞を分離する方法の提供。 　以下の工程：１）配列番号１で示されるアミノ酸配列からなるポリペプチド；配列番号１で示されるアミノ酸配列と実質的に同一なアミノ酸配列からなるポリペプチド；及びそれらの部分ポリペプチドから選択されるアンカーポリペプチドとプロテインＧの抗体結合ドメインを有する抗体結合性ポリペプチドとの融合ポリペプチドを、その有機膜中に有する磁性細菌由来磁気微粒子に、標的細胞と結合し得る抗体を固定化する工程；２）該抗体を固定化した磁性細菌由来磁気微粒子に、標的細胞を接触させる工程；３）該標的細胞と結合した磁気微粒子を磁気回収する工程、を行うことを特徴とする、試料中の標的細胞の分離又は検出方法。
PCT/JP2008/003007 2007-10-26 2008-10-23 融合ポリペプチド結合磁気微粒子による細胞分離方法 WO2009054136A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007278362A JP2009106159A (ja) 2007-10-26 2007-10-26 融合ポリペプチド結合磁気微粒子による細胞分離方法
JP2007-278362 2007-10-26

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WO2009054136A1 true WO2009054136A1 (ja) 2009-04-30

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PCT/JP2008/003007 WO2009054136A1 (ja) 2007-10-26 2008-10-23 融合ポリペプチド結合磁気微粒子による細胞分離方法

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JP (1) JP2009106159A (ja)
WO (1) WO2009054136A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105572396A (zh) * 2016-03-17 2016-05-11 北京中科圆融生物科技发展有限公司 表达重组蛋白g的细菌磁颗粒及其应用

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101213971B1 (ko) 2010-09-14 2012-12-20 서울대학교산학협력단 세포소기관 원격 이동방법, 장치 및 이를 위한 자성입자 복합체
KR101230384B1 (ko) * 2010-10-11 2013-02-07 서울대학교산학협력단 자성입자 및 외부자기장에 의한 세포 증식, 사멸 조절 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006314314A (ja) * 2005-04-15 2006-11-24 Tokyo Univ Of Agriculture & Technology 磁気微粒子およびその製造方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006314314A (ja) * 2005-04-15 2006-11-24 Tokyo Univ Of Agriculture & Technology 磁気微粒子およびその製造方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MURAMATSU M.: "Shin'idenshi Kogaku Handbook", 10 September 1999, YODOSHA CO., LTD., article "Experimental Medicine separate", pages: 170 - 171 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105572396A (zh) * 2016-03-17 2016-05-11 北京中科圆融生物科技发展有限公司 表达重组蛋白g的细菌磁颗粒及其应用
CN105572396B (zh) * 2016-03-17 2017-12-08 北京中科圆融生物科技发展有限公司 表达重组蛋白g的细菌磁颗粒及其应用

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