WO2009040508A3 - Methods of detection and enrichment of prpsc by immunoprecipitation and elisa - Google Patents
Methods of detection and enrichment of prpsc by immunoprecipitation and elisa Download PDFInfo
- Publication number
- WO2009040508A3 WO2009040508A3 PCT/GB2008/003215 GB2008003215W WO2009040508A3 WO 2009040508 A3 WO2009040508 A3 WO 2009040508A3 GB 2008003215 W GB2008003215 W GB 2008003215W WO 2009040508 A3 WO2009040508 A3 WO 2009040508A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- prpsc
- detection
- methods
- sample
- reagent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2872—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against prion molecules, e.g. CD230
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
- G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/28—Neurological disorders
- G01N2800/2814—Dementia; Cognitive disorders
- G01N2800/2828—Prion diseases
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a method of extracting or enriching PrP sc from a sample, said method comprising: (i) contacting said sample with a reagent which selectively binds PrPsc (ii) incubating said reagent with said sample (iii) separating or concentrating said reagent from said sample wherein said reagent comprises an ICSM33 antibody or a fragment or fusion thereof. The invention also relates to methods of detection of PrPsc, and to buffers and materials for use in same.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0718603.4 | 2007-09-24 | ||
GB0718603A GB0718603D0 (en) | 2007-09-24 | 2007-09-24 | Methods |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009040508A2 WO2009040508A2 (en) | 2009-04-02 |
WO2009040508A3 true WO2009040508A3 (en) | 2009-05-22 |
Family
ID=38670413
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2008/003215 WO2009040508A2 (en) | 2007-09-24 | 2008-09-24 | Methods of detection and enrichment of prpsc by immunoprecipitation and elisa |
Country Status (2)
Country | Link |
---|---|
GB (1) | GB0718603D0 (en) |
WO (1) | WO2009040508A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201015569D0 (en) | 2010-09-16 | 2010-10-27 | Medical Res Council | Blood assay for prions |
AU2012207454B2 (en) * | 2011-01-18 | 2016-11-17 | Prionics Ag | Methods for amplification and detection of prions |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003096023A1 (en) * | 2002-05-07 | 2003-11-20 | Medical Research Council | Sarcosyl buffer and its use for the purification of prion protein |
US20050282238A1 (en) * | 2003-09-23 | 2005-12-22 | Bowen David J | High-sensitivity chemiluminescent ELISA prion detection method |
WO2006088281A1 (en) * | 2005-02-19 | 2006-08-24 | Peoplebio, Inc. | Method for differentially detecting multimeric form from monomeric form of multimer-forming polypeptides |
-
2007
- 2007-09-24 GB GB0718603A patent/GB0718603D0/en not_active Ceased
-
2008
- 2008-09-24 WO PCT/GB2008/003215 patent/WO2009040508A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003096023A1 (en) * | 2002-05-07 | 2003-11-20 | Medical Research Council | Sarcosyl buffer and its use for the purification of prion protein |
US20050282238A1 (en) * | 2003-09-23 | 2005-12-22 | Bowen David J | High-sensitivity chemiluminescent ELISA prion detection method |
WO2006088281A1 (en) * | 2005-02-19 | 2006-08-24 | Peoplebio, Inc. | Method for differentially detecting multimeric form from monomeric form of multimer-forming polypeptides |
Non-Patent Citations (5)
Title |
---|
BERINGUE VINCENT ET AL: "Regional heterogeneity of cellular prion protein isoforms in the mouse brain.", BRAIN : A JOURNAL OF NEUROLOGY SEP 2003, vol. 126, no. Pt 9, 23 June 2003 (2003-06-23), online, pages 2065 - 2073, XP002514519, ISSN: 0006-8950 * |
KHALILI-SHIRAZI ET AL: "beta-PrP form of human prion protein stimulates production of monoclonal antibodies to epitope 91-110 that recognise native PrP<Sc>", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - PROTEINS & PROTEOMICS, ELSEVIER, vol. 1774, no. 11, 6 September 2007 (2007-09-06), online, pages 1438 - 1450, XP022356032, ISSN: 1570-9639 * |
PAQUET SOPHIE ET AL: "Efficient dissemination of prions through preferential transmission to nearby cells.", THE JOURNAL OF GENERAL VIROLOGY FEB 2007, vol. 88, no. Pt 2, February 2007 (2007-02-01), pages 706 - 713, XP002514520, ISSN: 0022-1317 * |
PRUSINER S B ET AL: "FURTHER PURIFICATION AND CHARCATERIZATION OF SCRAPIE PRIONS", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA.; US, vol. 21, no. 26, 21 December 1982 (1982-12-21), pages 6942 - 6950, XP001105221, ISSN: 0006-2960 * |
VARIOUS: "ABN Abstract. The Association of British Neurologists Autumn Meeting, London, UK, 4-6 October 2006", JOURNAL OF NEUROLOGY NEUROSURGERY & PSYCHIATRY; 20061004 - 20061006, vol. 78, no. 2, 4 October 2006 (2006-10-04) - 6 October 2006 (2006-10-06), pages 206 - 22, XP008096950, ISSN: 0022-3050 * |
Also Published As
Publication number | Publication date |
---|---|
GB0718603D0 (en) | 2007-10-31 |
WO2009040508A2 (en) | 2009-04-02 |
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