WO2009040508A3 - Methods of detection and enrichment of prpsc by immunoprecipitation and elisa - Google Patents

Methods of detection and enrichment of prpsc by immunoprecipitation and elisa Download PDF

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Publication number
WO2009040508A3
WO2009040508A3 PCT/GB2008/003215 GB2008003215W WO2009040508A3 WO 2009040508 A3 WO2009040508 A3 WO 2009040508A3 GB 2008003215 W GB2008003215 W GB 2008003215W WO 2009040508 A3 WO2009040508 A3 WO 2009040508A3
Authority
WO
WIPO (PCT)
Prior art keywords
prpsc
detection
methods
sample
reagent
Prior art date
Application number
PCT/GB2008/003215
Other languages
French (fr)
Other versions
WO2009040508A2 (en
Inventor
John Collinge
Graham Stuart Jackson
Original Assignee
Medical Res Council
John Collinge
Graham Stuart Jackson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, John Collinge, Graham Stuart Jackson filed Critical Medical Res Council
Publication of WO2009040508A2 publication Critical patent/WO2009040508A2/en
Publication of WO2009040508A3 publication Critical patent/WO2009040508A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2872—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against prion molecules, e.g. CD230
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G01N2800/2828—Prion diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method of extracting or enriching PrP sc from a sample, said method comprising: (i) contacting said sample with a reagent which selectively binds PrPsc (ii) incubating said reagent with said sample (iii) separating or concentrating said reagent from said sample wherein said reagent comprises an ICSM33 antibody or a fragment or fusion thereof. The invention also relates to methods of detection of PrPsc, and to buffers and materials for use in same.
PCT/GB2008/003215 2007-09-24 2008-09-24 Methods of detection and enrichment of prpsc by immunoprecipitation and elisa WO2009040508A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0718603.4 2007-09-24
GB0718603A GB0718603D0 (en) 2007-09-24 2007-09-24 Methods

Publications (2)

Publication Number Publication Date
WO2009040508A2 WO2009040508A2 (en) 2009-04-02
WO2009040508A3 true WO2009040508A3 (en) 2009-05-22

Family

ID=38670413

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2008/003215 WO2009040508A2 (en) 2007-09-24 2008-09-24 Methods of detection and enrichment of prpsc by immunoprecipitation and elisa

Country Status (2)

Country Link
GB (1) GB0718603D0 (en)
WO (1) WO2009040508A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201015569D0 (en) 2010-09-16 2010-10-27 Medical Res Council Blood assay for prions
AU2012207454B2 (en) * 2011-01-18 2016-11-17 Prionics Ag Methods for amplification and detection of prions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003096023A1 (en) * 2002-05-07 2003-11-20 Medical Research Council Sarcosyl buffer and its use for the purification of prion protein
US20050282238A1 (en) * 2003-09-23 2005-12-22 Bowen David J High-sensitivity chemiluminescent ELISA prion detection method
WO2006088281A1 (en) * 2005-02-19 2006-08-24 Peoplebio, Inc. Method for differentially detecting multimeric form from monomeric form of multimer-forming polypeptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003096023A1 (en) * 2002-05-07 2003-11-20 Medical Research Council Sarcosyl buffer and its use for the purification of prion protein
US20050282238A1 (en) * 2003-09-23 2005-12-22 Bowen David J High-sensitivity chemiluminescent ELISA prion detection method
WO2006088281A1 (en) * 2005-02-19 2006-08-24 Peoplebio, Inc. Method for differentially detecting multimeric form from monomeric form of multimer-forming polypeptides

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BERINGUE VINCENT ET AL: "Regional heterogeneity of cellular prion protein isoforms in the mouse brain.", BRAIN : A JOURNAL OF NEUROLOGY SEP 2003, vol. 126, no. Pt 9, 23 June 2003 (2003-06-23), online, pages 2065 - 2073, XP002514519, ISSN: 0006-8950 *
KHALILI-SHIRAZI ET AL: "beta-PrP form of human prion protein stimulates production of monoclonal antibodies to epitope 91-110 that recognise native PrP<Sc>", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - PROTEINS & PROTEOMICS, ELSEVIER, vol. 1774, no. 11, 6 September 2007 (2007-09-06), online, pages 1438 - 1450, XP022356032, ISSN: 1570-9639 *
PAQUET SOPHIE ET AL: "Efficient dissemination of prions through preferential transmission to nearby cells.", THE JOURNAL OF GENERAL VIROLOGY FEB 2007, vol. 88, no. Pt 2, February 2007 (2007-02-01), pages 706 - 713, XP002514520, ISSN: 0022-1317 *
PRUSINER S B ET AL: "FURTHER PURIFICATION AND CHARCATERIZATION OF SCRAPIE PRIONS", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, EASTON, PA.; US, vol. 21, no. 26, 21 December 1982 (1982-12-21), pages 6942 - 6950, XP001105221, ISSN: 0006-2960 *
VARIOUS: "ABN Abstract. The Association of British Neurologists Autumn Meeting, London, UK, 4-6 October 2006", JOURNAL OF NEUROLOGY NEUROSURGERY & PSYCHIATRY; 20061004 - 20061006, vol. 78, no. 2, 4 October 2006 (2006-10-04) - 6 October 2006 (2006-10-06), pages 206 - 22, XP008096950, ISSN: 0022-3050 *

Also Published As

Publication number Publication date
GB0718603D0 (en) 2007-10-31
WO2009040508A2 (en) 2009-04-02

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