WO2009035107A1 - 非天然型抗生物質の製造方法 - Google Patents

非天然型抗生物質の製造方法 Download PDF

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Publication number
WO2009035107A1
WO2009035107A1 PCT/JP2008/066596 JP2008066596W WO2009035107A1 WO 2009035107 A1 WO2009035107 A1 WO 2009035107A1 JP 2008066596 W JP2008066596 W JP 2008066596W WO 2009035107 A1 WO2009035107 A1 WO 2009035107A1
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WO
WIPO (PCT)
Prior art keywords
transformant
production
natural
producing
daunorubicin
Prior art date
Application number
PCT/JP2008/066596
Other languages
English (en)
French (fr)
Inventor
Naomi Sumida
Manabu Watanabe
Koji Yanai
Original Assignee
Meiji Seika Kaisha, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meiji Seika Kaisha, Ltd. filed Critical Meiji Seika Kaisha, Ltd.
Priority to CN2008801071967A priority Critical patent/CN101802168B/zh
Priority to US12/678,101 priority patent/US8383392B2/en
Priority to EP08830223.7A priority patent/EP2192171B1/en
Priority to JP2009532254A priority patent/JP5422387B2/ja
Publication of WO2009035107A1 publication Critical patent/WO2009035107A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

　本来ダウノルビシンを生産する能力を有した放線菌に、L-エピバンコサミンの生合成に関わるケトレダクターゼ遺伝子を導入した形質転換体、及び、これを効率的な非天然型ダウノルビシン誘導体の製造方法を開示する。本形質転換体は、エピダウノルビシン等の非天然型ダウノルビシン誘導体を効率的に製造する能力を有する。
PCT/JP2008/066596 2007-09-14 2008-09-12 非天然型抗生物質の製造方法 WO2009035107A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN2008801071967A CN101802168B (zh) 2007-09-14 2008-09-12 非天然型抗生素的制造方法
US12/678,101 US8383392B2 (en) 2007-09-14 2008-09-12 Transformant and method for production of non-natural antibiotic
EP08830223.7A EP2192171B1 (en) 2007-09-14 2008-09-12 Method for production of non-natural antibiotic
JP2009532254A JP5422387B2 (ja) 2007-09-14 2008-09-12 非天然型抗生物質の製造方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007239001 2007-09-14
JP2007-239001 2007-09-14

Publications (1)

Publication Number Publication Date
WO2009035107A1 true WO2009035107A1 (ja) 2009-03-19

Family

ID=40452113

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/066596 WO2009035107A1 (ja) 2007-09-14 2008-09-12 非天然型抗生物質の製造方法

Country Status (6)

Country Link
US (1) US8383392B2 (ja)
EP (1) EP2192171B1 (ja)
JP (1) JP5422387B2 (ja)
KR (1) KR101602195B1 (ja)
CN (1) CN101802168B (ja)
WO (1) WO2009035107A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010252657A (ja) * 2009-04-22 2010-11-11 Meiji Seika Kaisha Ltd ケトレダクターゼ変異体
WO2011145211A1 (ja) 2010-05-21 2011-11-24 明治製菓株式会社 ケトレダクターゼ変異体
WO2016159092A1 (ja) * 2015-03-30 2016-10-06 Ｍｅｉｊｉ Ｓｅｉｋａファルマ株式会社 エピルビシンの製造方法およびその新規な製造中間体

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105861455B (zh) * 2016-05-16 2018-10-26 浙江海正药业股份有限公司 DoxA蛋白突变体及其编码基因与应用
CN107541481B (zh) * 2016-06-23 2021-07-02 上海医药工业研究院 一种产表阿霉素的基因工程菌及其应用
CN107541535B (zh) * 2016-06-23 2021-10-29 上海医药工业研究院 发酵培养基及生产表阿霉素的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002512784A (ja) * 1998-04-24 2002-05-08 フアルマシア・エ・アツプジヨン・エツセ・ピー・アー ドキソルビシンの製造方法
WO2006111561A1 (en) 2005-04-21 2006-10-26 Dsm Ip Assets B.V. Improved microbial production of anthracyclins
JP2007261976A (ja) * 2006-03-28 2007-10-11 Meiji Seika Kaisha Ltd 抗腫瘍性アンスラサイクリン系抗生物質の製造法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2252352B1 (ja) * 1973-11-27 1978-11-03 Rhone Poulenc Ind

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002512784A (ja) * 1998-04-24 2002-05-08 フアルマシア・エ・アツプジヨン・エツセ・ピー・アー ドキソルビシンの製造方法
WO2006111561A1 (en) 2005-04-21 2006-10-26 Dsm Ip Assets B.V. Improved microbial production of anthracyclins
JP2007261976A (ja) * 2006-03-28 2007-10-11 Meiji Seika Kaisha Ltd 抗腫瘍性アンスラサイクリン系抗生物質の製造法

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
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BIERMAN, M. ET AL., GENE, (NETHERLANDS), vol. 116, 1992, pages 43 - 49
CHEN H ET AL: "Deoxysugars in Glycopeptide Antibiotics: Enzymatic Synthesis of TDP-L-Epivancosamine in Chloroeremomycin Biosynthesis", PROC. NATL. ACAD. SCI., vol. 97, no. 22, 2000, USA, pages 11942 - 11947, XP008131368 *
CHEN, H. ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (U.S.A.), vol. 97, 2000, pages 11942 - 11947
HUTCHINSON CR ET AL: "Genetic Engineering of Doxorubicin Production in Streptomyces peucetius", J. IND. MICROBIOL. BIOTECHNOL., vol. 23, no. 1, 1999, pages 647 - 652, XP002342779 *
KOMIYAMA, T. ET AL., THE JOURNAL OF ANTIBIOTICS, (JAPAN), vol. 30, 1977, pages 619 - 621
LESKIW, B. K. ET AL., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (U.S.A.), vol. 88, 1991, pages 2461 - 2465
MADDURI K ET AL: "Production of the Antitumor Drug Epirubicin (4'-Epidoxorubicin) and Its Precursos by a Genetically Engineered Strain of Streptomyces peucetius", NAT. BIOTECHNOL., vol. 16, no. 1, 1998, pages 69 - 74, XP002210929 *
MADDURI, K. ET AL., NATURE BIOTECHNOLOGY, (U.S.A.), vol. 16, 1998, pages 69 - 74
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SCHMITT-JOHN, T.; ENGELS, J. W., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, (GERMANY), vol. 36, 1992, pages 493 - 498
SCIENCE, vol. 22'7, 1985, pages 1435 - 1441
See also references of EP2192171A4
SHANG K ET AL: "Production of 4'-Epidaunorubicin by Metabolic Engineering of Streptomyces coeruleorubidus Strain SIPI-1482", WORLD J. MICROBIOL. BIOTECHNOL., vol. 24, no. 7, July 2008 (2008-07-01), pages 1107 - 1113, XP019617033 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010252657A (ja) * 2009-04-22 2010-11-11 Meiji Seika Kaisha Ltd ケトレダクターゼ変異体
WO2011145211A1 (ja) 2010-05-21 2011-11-24 明治製菓株式会社 ケトレダクターゼ変異体
CN102906258A (zh) * 2010-05-21 2013-01-30 明治制果药业株式会社 酮还原酶突变体
US20130116416A1 (en) * 2010-05-21 2013-05-09 Meiji Seika Pharma, Co., Ltd. Ketoreductase mutant
KR20130090774A (ko) 2010-05-21 2013-08-14 메이지 세이카 파루마 가부시키가이샤 케토리덕타아제 변이체
US8697409B2 (en) 2010-05-21 2014-04-15 Meiji Seika Pharma Co., Ltd. Ketoreductase mutant
CN102906258B (zh) * 2010-05-21 2015-06-17 明治制果药业株式会社 酮还原酶突变体
KR101718985B1 (ko) * 2010-05-21 2017-03-22 메이지 세이카 파루마 가부시키가이샤 케토리덕타아제 변이체
WO2016159092A1 (ja) * 2015-03-30 2016-10-06 Ｍｅｉｊｉ Ｓｅｉｋａファルマ株式会社 エピルビシンの製造方法およびその新規な製造中間体
JPWO2016159092A1 (ja) * 2015-03-30 2018-01-25 Ｍｅｉｊｉ Ｓｅｉｋａファルマ株式会社 エピルビシンの製造方法およびその新規な製造中間体
CN107683284A (zh) * 2015-03-30 2018-02-09 明治制果药业株式会社 表柔比星的制造方法及其新的制造中间体
US10301343B2 (en) 2015-03-30 2019-05-28 Meiji Seika Pharma Co., Ltd. Method of producing epirubicin and novel production intermediate thereof

Also Published As

Publication number Publication date
JP5422387B2 (ja) 2014-02-19
CN101802168B (zh) 2013-06-12
CN101802168A (zh) 2010-08-11
JPWO2009035107A1 (ja) 2010-12-24
US20100255543A1 (en) 2010-10-07
EP2192171B1 (en) 2014-03-05
EP2192171A1 (en) 2010-06-02
KR20100072023A (ko) 2010-06-29
KR101602195B1 (ko) 2016-03-10
EP2192171A4 (en) 2012-02-01
US8383392B2 (en) 2013-02-26

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