WO2008146754A1 - 反応液用容器、及びそれを用いる反応促進装置、並びにその方法 - Google Patents

反応液用容器、及びそれを用いる反応促進装置、並びにその方法 Download PDF

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Publication number
WO2008146754A1
WO2008146754A1 PCT/JP2008/059583 JP2008059583W WO2008146754A1 WO 2008146754 A1 WO2008146754 A1 WO 2008146754A1 JP 2008059583 W JP2008059583 W JP 2008059583W WO 2008146754 A1 WO2008146754 A1 WO 2008146754A1
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WIPO (PCT)
Prior art keywords
reaction mixture
liquid reaction
container
heater
liquid
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Application number
PCT/JP2008/059583
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English (en)
French (fr)
Inventor
Takashi Kodama
Tomoko Kodama
Kunio Tsuboi
Original Assignee
Trust Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trust Co., Ltd. filed Critical Trust Co., Ltd.
Priority to JP2009516305A priority Critical patent/JPWO2008146754A1/ja
Priority to US12/601,469 priority patent/US20100279392A1/en
Priority to CN200880023755A priority patent/CN101802163A/zh
Priority to EP08764629A priority patent/EP2182049A1/en
Publication of WO2008146754A1 publication Critical patent/WO2008146754A1/ja

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones

Abstract

【解決手段】　所定温度のヒータに接触させ、反応液の核酸増幅反応を促進するための反応液用容器であって、表面と裏面とを有し、前記いずれかの面もしくはその両面がヒータに対向した状態に保持されるように構成された基板と、この基板の面方向に互いに離間、独立して設けられ、それぞれに前記反応液を保持し液密に密閉されるウエルと、を有し、前記ウエルは、前記基板に設けられた開口部と、この開口部の基板表面側および裏面側を閉塞する閉塞部材とからなり、少なくとも前記ヒータと接する側の面を閉塞する閉塞部材は厚さ１０～３００μｍの伸縮性フィルム部分を有するものであることを特徴とする反応液用容器が提供される。
PCT/JP2008/059583 2007-05-23 2008-05-23 反応液用容器、及びそれを用いる反応促進装置、並びにその方法 WO2008146754A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2009516305A JPWO2008146754A1 (ja) 2007-05-23 2008-05-23 反応液用容器、及びそれを用いる反応促進装置、並びにその方法
US12/601,469 US20100279392A1 (en) 2007-05-23 2008-05-23 Container for Liquid Reaction Mixture, Reaction-Promoting Device Using the Same and Method Therefor
CN200880023755A CN101802163A (zh) 2007-05-23 2008-05-23 反应液用容器，使用这种容器的反应促进装置，及其方法
EP08764629A EP2182049A1 (en) 2007-05-23 2008-05-23 Container for liquid reaction mixture, reaction-promoting device using the same and method therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007137299 2007-05-23
JP2007-137299 2007-05-23

Publications (1)

Publication Number Publication Date
WO2008146754A1 true WO2008146754A1 (ja) 2008-12-04

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Country Status (5)

Country Link
US (1) US20100279392A1 (ja)
EP (1) EP2182049A1 (ja)
JP (1) JPWO2008146754A1 (ja)
CN (1) CN101802163A (ja)
WO (1) WO2008146754A1 (ja)

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JP2010172270A (ja) * 2009-01-29 2010-08-12 Soka Univ マイクロ反応容器及びマイクロ反応容器を用いたポリメラーゼ連鎖反応方法
WO2011054353A1 (de) * 2009-11-05 2011-05-12 Friz Biochem Gesellschaft Für Bioanalytik Mbh Vorrichtung zur durchführung einer pcr
JP2011239688A (ja) * 2010-05-14 2011-12-01 Hitachi High-Technologies Corp 核酸増幅装置及び核酸増幅方法
WO2012063647A1 (ja) * 2010-11-08 2012-05-18 株式会社日立ハイテクノロジーズ 反応プレートアセンブリ、反応プレート及び核酸分析装置
JP2012103019A (ja) * 2010-11-08 2012-05-31 Hitachi High-Technologies Corp 反応プレートアセンブリ、反応プレート及び核酸分析装置
JP2012100549A (ja) * 2010-11-08 2012-05-31 Hitachi High-Technologies Corp 核酸分析装置
JP2012187054A (ja) * 2011-03-11 2012-10-04 Hitachi High-Technologies Corp 核酸増幅反応装置
JP2013000012A (ja) * 2011-06-14 2013-01-07 Hitachi High-Technologies Corp 反応デバイス及びこれを用いた反応装置
JP2013208127A (ja) * 2013-06-06 2013-10-10 Soka Univ マイクロ反応容器及びマイクロ反応容器を用いたポリメラーゼ連鎖反応方法
JP2013252091A (ja) * 2012-06-06 2013-12-19 Seiko Epson Corp 熱サイクル装置
WO2014020977A1 (ja) * 2012-08-03 2014-02-06 ソニー株式会社 核酸分析装置、核酸分析用マイクロチップ及び該装置におけるマイクロチップ搭載方法
JP2015522168A (ja) * 2012-07-09 2015-08-03 バイオカーティス エヌ ヴイ 使い捨て可能な診断カートリッジ用ヒータ
WO2016047744A1 (ja) * 2014-09-26 2016-03-31 東洋紡株式会社 反応促進装置及び核酸検査装置
JP2016086751A (ja) * 2014-11-06 2016-05-23 東洋紡株式会社 反応促進装置及び核酸検査装置
US9427738B2 (en) 2012-03-30 2016-08-30 Seiko Epson Corporation Thermal cycler and control method of thermal cycler
US9789459B2 (en) 2014-02-20 2017-10-17 Seiko Epson Corporation Nucleic acid amplification reaction vessel and nucleic acid amplification reaction apparatus
JP2018072302A (ja) * 2016-11-04 2018-05-10 シスメックス株式会社 液体封入カートリッジ、液体封入カートリッジの製造方法および送液方法
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Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010172270A (ja) * 2009-01-29 2010-08-12 Soka Univ マイクロ反応容器及びマイクロ反応容器を用いたポリメラーゼ連鎖反応方法
WO2011054353A1 (de) * 2009-11-05 2011-05-12 Friz Biochem Gesellschaft Für Bioanalytik Mbh Vorrichtung zur durchführung einer pcr
JP2011239688A (ja) * 2010-05-14 2011-12-01 Hitachi High-Technologies Corp 核酸増幅装置及び核酸増幅方法
US9221055B2 (en) 2010-11-08 2015-12-29 Hitachi High-Technologies Corporation Reaction plate assembly, reaction plate and nucleic acid analysis device
WO2012063647A1 (ja) * 2010-11-08 2012-05-18 株式会社日立ハイテクノロジーズ 反応プレートアセンブリ、反応プレート及び核酸分析装置
JP2012100549A (ja) * 2010-11-08 2012-05-31 Hitachi High-Technologies Corp 核酸分析装置
JP2012103019A (ja) * 2010-11-08 2012-05-31 Hitachi High-Technologies Corp 反応プレートアセンブリ、反応プレート及び核酸分析装置
JP2012187054A (ja) * 2011-03-11 2012-10-04 Hitachi High-Technologies Corp 核酸増幅反応装置
JP2013000012A (ja) * 2011-06-14 2013-01-07 Hitachi High-Technologies Corp 反応デバイス及びこれを用いた反応装置
US9427738B2 (en) 2012-03-30 2016-08-30 Seiko Epson Corporation Thermal cycler and control method of thermal cycler
JP2013252091A (ja) * 2012-06-06 2013-12-19 Seiko Epson Corp 熱サイクル装置
JP2015522168A (ja) * 2012-07-09 2015-08-03 バイオカーティス エヌ ヴイ 使い捨て可能な診断カートリッジ用ヒータ
WO2014020977A1 (ja) * 2012-08-03 2014-02-06 ソニー株式会社 核酸分析装置、核酸分析用マイクロチップ及び該装置におけるマイクロチップ搭載方法
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