WO2008126151A1 - 質量分析データ解析方法及び装置 - Google Patents

質量分析データ解析方法及び装置 Download PDF

Info

Publication number
WO2008126151A1
WO2008126151A1 PCT/JP2007/000366 JP2007000366W WO2008126151A1 WO 2008126151 A1 WO2008126151 A1 WO 2008126151A1 JP 2007000366 W JP2007000366 W JP 2007000366W WO 2008126151 A1 WO2008126151 A1 WO 2008126151A1
Authority
WO
WIPO (PCT)
Prior art keywords
mass
peaks
mass spectrum
window
aggregating
Prior art date
Application number
PCT/JP2007/000366
Other languages
English (en)
French (fr)
Inventor
Shigeki Kajihara
Original Assignee
Shimadzu Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corporation filed Critical Shimadzu Corporation
Priority to US12/594,480 priority Critical patent/US8119982B2/en
Priority to PCT/JP2007/000366 priority patent/WO2008126151A1/ja
Priority to JP2009508705A priority patent/JP4952788B2/ja
Publication of WO2008126151A1 publication Critical patent/WO2008126151A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F2218/00—Aspects of pattern recognition specially adapted for signal processing
    • G06F2218/08—Feature extraction
    • G06F2218/10—Feature extraction by analysing the shape of a waveform, e.g. extracting parameters relating to peaks

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

　試料上の二次元領域を細かく区分した各微小領域（ピクセル）に対して得られる質量スペクトルデータを解析処理しタンパク質を同定するに際し、まず各ピクセルの質量スペクトルに現れるピークを含むように質量ウインドウを設定し（Ｓ１０）、各質量ウインドウ内に入るピークのイオン強度の積算値を計算する（Ｓ１１）。そして、質量ウインドウ毎に各ピクセルの強度積算値を集めてマッピング画像を作成し（Ｓ１２）、マッピング画像の類似性を判断して質量ウインドウをグループ分けする（Ｓ１３、Ｓ１４）。同一グループに入る質量ウインドウ内のピークは同一種類の物質に由来するとみなし、それらピークを集めて質量スペクトルを作成し（Ｓ１５）、該スペクトルに基づいてＰＭＦ法などによりタンパク質を同定する（Ｓ１６、Ｓ１７）。これにより、複数種類のタンパク質が混在している場合でも、同定精度を高めることができる。　
PCT/JP2007/000366 2007-04-04 2007-04-04 質量分析データ解析方法及び装置 WO2008126151A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/594,480 US8119982B2 (en) 2007-04-04 2007-04-04 Method and system for mass spectrometry data analysis
PCT/JP2007/000366 WO2008126151A1 (ja) 2007-04-04 2007-04-04 質量分析データ解析方法及び装置
JP2009508705A JP4952788B2 (ja) 2007-04-04 2007-04-04 質量分析データ解析方法及び装置

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/000366 WO2008126151A1 (ja) 2007-04-04 2007-04-04 質量分析データ解析方法及び装置

Publications (1)

Publication Number Publication Date
WO2008126151A1 true WO2008126151A1 (ja) 2008-10-23

Family

ID=39863336

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/000366 WO2008126151A1 (ja) 2007-04-04 2007-04-04 質量分析データ解析方法及び装置

Country Status (3)

Country Link
US (1) US8119982B2 (ja)
JP (1) JP4952788B2 (ja)
WO (1) WO2008126151A1 (ja)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012517588A (ja) * 2009-02-06 2012-08-02 マイクロマス・ユー・ケイ・リミテツド 質量分析方法
JP2012247198A (ja) * 2011-05-25 2012-12-13 Shimadzu Corp 質量分析データ解析方法及び解析装置
JP2013178233A (ja) * 2012-01-30 2013-09-09 Canon Inc 生体組織画像のノイズ低減処理方法及び装置
JP2014508946A (ja) * 2011-03-21 2014-04-10 イマビオテク サンプル中のターゲット分子を検出及び定量化するための方法
JP2017173103A (ja) * 2016-03-23 2017-09-28 日本電子株式会社 質量分析装置
WO2017195271A1 (ja) * 2016-05-10 2017-11-16 株式会社島津製作所 イメージング質量分析装置
WO2018037491A1 (ja) * 2016-08-24 2018-03-01 株式会社島津製作所 イメージング質量分析装置
WO2018037569A1 (ja) * 2016-08-26 2018-03-01 株式会社島津製作所 イメージング質量分析データ処理装置及び方法
CN113740408A (zh) * 2020-05-28 2021-12-03 塞莫费雪科学（不来梅）有限公司 用于确定ms扫描数据中的干扰、过滤离子并对样品进行质谱分析的方法和装置

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007024857B4 (de) * 2007-05-29 2017-11-02 Bruker Daltonik Gmbh Bildgebende Massenspektrometrie für kleine Moleküle in flächigen Proben
US8324569B2 (en) * 2008-07-03 2012-12-04 Shimadzu Corporation Mass spectrometer
JP5359924B2 (ja) * 2010-02-18 2013-12-04 株式会社島津製作所 質量分析装置
JP6044385B2 (ja) * 2013-02-26 2016-12-14 株式会社島津製作所 タンデム型質量分析装置
JP2017511571A (ja) * 2014-04-02 2017-04-20 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 質量分析法によるサブミクロン元素画像解析の装置及び方法
WO2017158673A1 (ja) * 2016-03-14 2017-09-21 株式会社島津製作所 質量分析データ解析装置及び質量分析データ解析用プログラム
JP6939912B2 (ja) * 2018-02-02 2021-09-22 株式会社島津製作所 イメージング質量分析用データ処理装置
WO2019229897A1 (ja) * 2018-05-30 2019-12-05 株式会社島津製作所 イメージングデータ処理装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006010658A (ja) * 2003-07-02 2006-01-12 Canon Inc 情報取得方法、情報取得装置及び検出方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5808300A (en) * 1996-05-10 1998-09-15 Board Of Regents, The University Of Texas System Method and apparatus for imaging biological samples with MALDI MS
JP3658397B2 (ja) * 2002-06-28 2005-06-08 キヤノン株式会社 飛行時間型二次イオン質量分析法による素子の情報取得方法、および、情報取得装置
CN1813190B (zh) 2003-07-02 2011-06-29 佳能株式会社 信息获取方法
JP2006284509A (ja) 2005-04-04 2006-10-19 Shimadzu Corp 質量分析システム
US7872223B2 (en) * 2006-04-07 2011-01-18 Shimadzu Corporation Mass spectrometer
JP4973659B2 (ja) * 2006-05-30 2012-07-11 株式会社島津製作所 質量分析装置

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006010658A (ja) * 2003-07-02 2006-01-12 Canon Inc 情報取得方法、情報取得装置及び検出方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AOYAGI S.: "Bio Device no Hiko Jikan Gata Niji Ion Shitsuryo Bunseki Imaging (Time-of-Flight Secondary Ion Mass Spectrometry Imaging of Biodevices)", J. MASS. SPECTROM. SOC. JPN., vol. 55, no. 1, 1 February 2007 (2007-02-01), pages 33 - 38, XP003022994 *
BÖRNER K. ET AL.: "Molecular imaging of lipids in cells and tissues", INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, vol. 260, no. 2-3, 1 February 2007 (2007-02-01), pages 128 - 136, XP005822709 *
GARRET T.J. ET AL.: "Imaging of small molecules in tissue sections with a new intermediate-pressure MALDI linear ion trap mass spectrometer", INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, vol. 260, no. 2-3, 1 February 2007 (2007-02-01), pages 166 - 176, XP005822713 *
TABAN I.M. ET AL.: "Imaging of Peptides in the Rat Brain Using MALDI-FTICR Mass Spectrometry", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 18, no. 1, January 2007 (2007-01-01), pages 145 - 151, XP005823248 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012517588A (ja) * 2009-02-06 2012-08-02 マイクロマス・ユー・ケイ・リミテツド 質量分析方法
JP2014508946A (ja) * 2011-03-21 2014-04-10 イマビオテク サンプル中のターゲット分子を検出及び定量化するための方法
JP2012247198A (ja) * 2011-05-25 2012-12-13 Shimadzu Corp 質量分析データ解析方法及び解析装置
US9075782B2 (en) 2011-05-25 2015-07-07 Shimadzu Corporation Mass spectrometry data analysis method and analysis device
JP2013178233A (ja) * 2012-01-30 2013-09-09 Canon Inc 生体組織画像のノイズ低減処理方法及び装置
JP2017173103A (ja) * 2016-03-23 2017-09-28 日本電子株式会社 質量分析装置
WO2017195271A1 (ja) * 2016-05-10 2017-11-16 株式会社島津製作所 イメージング質量分析装置
JPWO2017195271A1 (ja) * 2016-05-10 2018-11-08 株式会社島津製作所 イメージング質量分析装置
WO2018037491A1 (ja) * 2016-08-24 2018-03-01 株式会社島津製作所 イメージング質量分析装置
JPWO2018037491A1 (ja) * 2016-08-24 2019-01-10 株式会社島津製作所 イメージング質量分析装置
WO2018037569A1 (ja) * 2016-08-26 2018-03-01 株式会社島津製作所 イメージング質量分析データ処理装置及び方法
JPWO2018037569A1 (ja) * 2016-08-26 2019-01-10 株式会社島津製作所 イメージング質量分析データ処理装置及び方法
CN113740408A (zh) * 2020-05-28 2021-12-03 塞莫费雪科学（不来梅）有限公司 用于确定ms扫描数据中的干扰、过滤离子并对样品进行质谱分析的方法和装置

Also Published As

Publication number Publication date
JP4952788B2 (ja) 2012-06-13
US8119982B2 (en) 2012-02-21
JPWO2008126151A1 (ja) 2010-07-15
US20100116981A1 (en) 2010-05-13

Similar Documents

Publication Publication Date Title
WO2008126151A1 (ja) 質量分析データ解析方法及び装置
EP2579211A3 (en) Graph-based segmentation integrating visible and NIR information
Norris et al. Processing MALDI mass spectra to improve mass spectral direct tissue analysis
Egertson et al. Multiplexed MS/MS for improved data-independent acquisition
SG10201900339QA (en) Computing device and method for detecting malicious domain names in a network traffic
WO2004070653A3 (en) Image analysis system and method
WO2005010492A3 (en) Classification of disease states using mass spectrometry data
EP2500841A3 (en) Video based matching and tracking
WO2016145331A8 (en) Methods for data-dependent mass spectrometry of mixed biomolecular analytes
WO2005067582A3 (en) Methods and apparatus for enhanced sample identification based on combined analytical techniques
Wang et al. Rapid identification and classification of Mycobacterium spp. using whole-cell protein barcodes with matrix assisted laser desorption ionization time of flight mass spectrometry in comparison with multigene phylogenetic analysis
WO2006113054A3 (en) Methods and apparatus for adaptive foreground background analysis
HK1091514A1 (en) System and method for rapidly identifying pathogens, bacteria and abnormal cells
WO2007148284A3 (en) A method, a system and a computer program for determining a threshold in an image comprising image values
WO2015112352A3 (en) Method and system for microbiome analysis
WO2007015746A3 (en) Method of rendering pixel images from abstract datasets
WO2008008867A3 (en) Data acquisition system and method for a spectrometer
WO2014201052A3 (en) Medical image processing method
EP2587406A3 (en) System and Method for Link Analysis Based on Image Processing
WO2018072862A8 (de) Verfahren zum auswerten von daten einer massenspektrometrie und massenspektrometrisches verfahren sowie ein maldi tof massenspektrometer
EP2731054A3 (en) Method and device for recognizing document image, and photographing method using the same
EP2975384B1 (en) Microparticle analyzing device, microparticle analyzing method, program, and microparticle analyzing system
SG127861A1 (en) in situ biomarker identification
WO2005009039A3 (en) System and method for isotopic signature and mass analysis
WO2005103835A3 (en) A system for analyzing an organic photoconducting drum and a method thereof

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07737023

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2009508705

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 12594480

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07737023

Country of ref document: EP

Kind code of ref document: A1