WO2008117792A1 - アデノシン三リン酸測定のための蛍光標識融合タンパク質 - Google Patents

アデノシン三リン酸測定のための蛍光標識融合タンパク質 Download PDF

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Publication number
WO2008117792A1
WO2008117792A1 PCT/JP2008/055535 JP2008055535W WO2008117792A1 WO 2008117792 A1 WO2008117792 A1 WO 2008117792A1 JP 2008055535 W JP2008055535 W JP 2008055535W WO 2008117792 A1 WO2008117792 A1 WO 2008117792A1
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WO
WIPO (PCT)
Prior art keywords
protein
atp
fluorescent labeled
assaying
fused protein
Prior art date
Application number
PCT/JP2008/055535
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English (en)
French (fr)
Inventor
Hiroyuki Noji
Hiromi Imamura
Ryota Iino
Yasuyuki Yamada
Original Assignee
The New Industry Research Organization
Osaka University
Rikkyo Gakuin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The New Industry Research Organization, Osaka University, Rikkyo Gakuin filed Critical The New Industry Research Organization
Priority to JP2009506339A priority Critical patent/JPWO2008117792A1/ja
Priority to EP08738830.2A priority patent/EP2141230B1/en
Priority to US12/594,198 priority patent/US8524447B2/en
Publication of WO2008117792A1 publication Critical patent/WO2008117792A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)

Abstract

　本発明は、操作が簡便であり、タンパク質濃度に依存することなく感度の高いＡＴＰの測定を可能とする物質を提供することを課題とし、さらには、該物質を用いたＡＴＰの測定方法を提供することを課題とする。かかる課題は、ＡＴＰの結合に応じて構造変化を起こしうるタンパク質、即ちＡＴＰ合成酵素のサブユニットであるεタンパク質に、蛍光共鳴エネルギー移動（ＦＲＥＴ）におけるドナー及びアクセプターとなりうる２種類の蛍光物質をそれぞれ結合させてなる蛍光標識融合タンパク質により解決され、さらには、該蛍光標識融合タンパク質を被験物質と接触させた後の蛍光スペクトルを測定することにより解決される。
PCT/JP2008/055535 2007-03-27 2008-03-25 アデノシン三リン酸測定のための蛍光標識融合タンパク質 WO2008117792A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009506339A JPWO2008117792A1 (ja) 2007-03-27 2008-03-25 アデノシン三リン酸測定のための蛍光標識融合タンパク質
EP08738830.2A EP2141230B1 (en) 2007-03-27 2008-03-25 Fluorescent labeled fused protein for assaying adenosine triphosphate
US12/594,198 US8524447B2 (en) 2007-03-27 2008-03-25 Fluorescently labeled fusion protein for assaying adenosine triphosphate

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-082560 2007-03-27
JP2007082560 2007-03-27

Publications (1)

Publication Number Publication Date
WO2008117792A1 true WO2008117792A1 (ja) 2008-10-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/055535 WO2008117792A1 (ja) 2007-03-27 2008-03-25 アデノシン三リン酸測定のための蛍光標識融合タンパク質

Country Status (4)

Country Link
US (1) US8524447B2 (ja)
EP (1) EP2141230B1 (ja)
JP (1) JPWO2008117792A1 (ja)
WO (1) WO2008117792A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015108102A1 (ja) * 2014-01-15 2015-07-23 国立大学法人 群馬大学 Ａｔｐ可視化動物およびその用途

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CN103207167B (zh) * 2012-12-25 2015-04-15 西安交通大学 用于快速检测线粒体内atp的荧光共振体系的制备方法
CN103207166B (zh) * 2012-12-25 2015-06-03 西安交通大学 用于快速检测atp的荧光共振体系的制备方法
US9541548B1 (en) 2013-09-05 2017-01-10 Tempo Bioscience, Inc. Human biosensors for detecting ATP
US20150017658A1 (en) * 2014-05-19 2015-01-15 National Institute Of Standards And Technology Stain, process for staining and acquiring normalized signal
KR20200128649A (ko) * 2018-03-09 2020-11-16 에센 인스트루먼츠, 인크. 디/비/에이/ 에센 바이오사이언스, 인크. 세포내 atp의 생세포 분석을 위한 방법 및 조성물
CN110057805B (zh) * 2019-06-24 2019-09-27 烟台大学 一种三磷酸腺苷荧光检测水产品新鲜度的方法

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JP3749628B2 (ja) * 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
US8076079B2 (en) * 2003-12-17 2011-12-13 Arizona Board of Regents, a body coporate acting for and on behalf of Arizona State University Single molecule detection using molecular motors
JP4878152B2 (ja) * 2005-12-07 2012-02-15 浜松ホトニクス株式会社 連結分子モーター及びａｔｐセンサー

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015108102A1 (ja) * 2014-01-15 2015-07-23 国立大学法人 群馬大学 Ａｔｐ可視化動物およびその用途
JPWO2015108102A1 (ja) * 2014-01-15 2017-03-23 国立大学法人群馬大学 Ａｔｐ可視化動物およびその用途
US11160879B2 (en) 2014-01-15 2021-11-02 Masamichi Yamamoto Transgenic animal for visualization of ATP and use thereof

Also Published As

Publication number Publication date
EP2141230A4 (en) 2010-07-28
EP2141230B1 (en) 2014-06-25
EP2141230A1 (en) 2010-01-06
US20100233692A1 (en) 2010-09-16
JPWO2008117792A1 (ja) 2010-07-15
US8524447B2 (en) 2013-09-03

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