WO2007028921A3 - Methode de detection d'interaction intracellulaire entre bio-molecules - Google Patents

Methode de detection d'interaction intracellulaire entre bio-molecules Download PDF

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Publication number
WO2007028921A3
WO2007028921A3 PCT/FR2006/050838 FR2006050838W WO2007028921A3 WO 2007028921 A3 WO2007028921 A3 WO 2007028921A3 FR 2006050838 W FR2006050838 W FR 2006050838W WO 2007028921 A3 WO2007028921 A3 WO 2007028921A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
detecting intracellular
intracellular interaction
interaction
detecting
Prior art date
Application number
PCT/FR2006/050838
Other languages
English (en)
Other versions
WO2007028921A2 (fr
Inventor
Iris Pribilla
Herve Bazin
Sraboni Ghose
Norbert Tinel
Michel Fink
Eric Trinquet
Gerard Mathis
Original Assignee
Cis Bio Int
Iris Pribilla
Herve Bazin
Sraboni Ghose
Norbert Tinel
Michel Fink
Eric Trinquet
Gerard Mathis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cis Bio Int, Iris Pribilla, Herve Bazin, Sraboni Ghose, Norbert Tinel, Michel Fink, Eric Trinquet, Gerard Mathis filed Critical Cis Bio Int
Priority to US12/065,706 priority Critical patent/US8470523B2/en
Priority to JP2008529668A priority patent/JP2009506784A/ja
Priority to EP06808277A priority patent/EP1929294A2/fr
Publication of WO2007028921A2 publication Critical patent/WO2007028921A2/fr
Publication of WO2007028921A3 publication Critical patent/WO2007028921A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Abstract

La présente invention concerne une méthode non microscopique, quantitative, de détection d'interactions intracellulaires entre bio-molécules, sur cellules vivantes, en réponse à une stimulation biologique ou pharmacologique, par effet de transfert d'énergie de proximité en temps résolu entre deux membres d'un couple donneur/accepteur de fluorescence. Applications : criblage à haut débit de molécules et détection des voies de signalisation intracellulaire
PCT/FR2006/050838 2005-09-05 2006-09-04 Methode de detection d'interaction intracellulaire entre bio-molecules WO2007028921A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/065,706 US8470523B2 (en) 2005-09-05 2006-09-04 Method for detecting intracellular interaction between biomolecules
JP2008529668A JP2009506784A (ja) 2005-09-05 2006-09-04 生体分子間の細胞内相互作用の検出方法
EP06808277A EP1929294A2 (fr) 2005-09-05 2006-09-04 Methode de detection d'interaction intracellulaire entre bio-molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0509060 2005-09-05
FR0509060A FR2890446B1 (fr) 2005-09-05 2005-09-05 Methode de detection d'interaction intracellulaire entre bio-molecules

Publications (2)

Publication Number Publication Date
WO2007028921A2 WO2007028921A2 (fr) 2007-03-15
WO2007028921A3 true WO2007028921A3 (fr) 2007-06-07

Family

ID=36571024

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2006/050838 WO2007028921A2 (fr) 2005-09-05 2006-09-04 Methode de detection d'interaction intracellulaire entre bio-molecules

Country Status (5)

Country Link
US (1) US8470523B2 (fr)
EP (1) EP1929294A2 (fr)
JP (1) JP2009506784A (fr)
FR (1) FR2890446B1 (fr)
WO (1) WO2007028921A2 (fr)

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FR2936245B1 (fr) * 2008-09-23 2012-07-06 Cis Bio Int Nouveaux substrats d'o6-alkylguanine-adn alkyltransferase et ses mutants.
NZ593294A (en) 2009-01-29 2013-04-26 Commw Scient Ind Res Org Measuring g protein coupled receptor activation
WO2013091661A2 (fr) * 2011-12-23 2013-06-27 Aarhus Universitet Etiquette d'affinité protéique résistante à la protéolyse
CN104568861A (zh) * 2013-10-25 2015-04-29 华东理工大学 一种基于双分子互补的共价修饰标记来检测蛋白相互作用的方法
CA2946378A1 (fr) 2014-04-22 2015-10-29 Q-State Biosciences, Inc. Analyse de composes pour traiter la douleur et des troubles sensoriels
WO2016054509A1 (fr) * 2014-10-02 2016-04-07 Q-State Biosciences, Inc. Systèmes et procédés d'évaluation de la communication inter-cellulaire
CA2986583A1 (fr) 2015-05-21 2016-11-24 Q-State Biosciences, Inc. Microscope optogenetique
JP2018532384A (ja) * 2015-09-04 2018-11-08 ザ スクリプス リサーチ インスティテュート 新規な抗生物質を同定するための方法および関連組成物
WO2019133976A1 (fr) * 2017-12-29 2019-07-04 Howard Hughes Medical Institute Indicateurs de calcium chimiogénétiques
CN113238053B (zh) * 2021-04-30 2022-05-13 四川大学华西医院 一种用于检测stat3二聚化的质粒

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Also Published As

Publication number Publication date
JP2009506784A (ja) 2009-02-19
FR2890446A1 (fr) 2007-03-09
EP1929294A2 (fr) 2008-06-11
WO2007028921A2 (fr) 2007-03-15
US8470523B2 (en) 2013-06-25
US20090215106A1 (en) 2009-08-27
FR2890446B1 (fr) 2008-04-18

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