WO2008035691A1

WO2008035691A1 - Moisturizing agent, cell-activating agent, skin-whitening agent, and antioxidant agent

Info

Publication number: WO2008035691A1
Application number: PCT/JP2007/068126
Authority: WO
Inventors: Akira Hatani; Yumiko Okumura; Hayami Maeda
Original assignee: Noevir Co., Ltd.
Priority date: 2006-09-20
Filing date: 2007-09-19
Publication date: 2008-03-27
Also published as: JP2008074738A; JP4515425B2

Abstract

An active ingredient having excellent moisturizing, cell-activating, skin-whitening or anti-oxidant effect is now found. Provided is a moisturizing agent, a cell-activating agent, a skin-whitening agent or an antioxidant agent which can be widely applied to the fields of external preparations to the skin and foods. An extract of a plant belonging to the genus Androsace, which has an excellent moisturizing, cell-activating, skin-whitening or anti-oxidant effect, is used as an active ingredient of a moisturizing agent, a cell-activating agent, a skin-whitening agent or an anti-oxidant agent. The extract of a plant belonging to the genus Androsace can be added to a composition such as an external preparation for the skin and a food. Thus, it becomes possible to provide various compositions having an excellent moisturizing, cell-activating, skin-whitening or anti-oxidant effect.

Description

Specification

Moisturizer, cell activator, whitening agent, and antioxidant

Technical field

[0001] The present invention relates to a moisturizer, cell activator, whitening agent, and antioxidant containing a plant extract as an active ingredient, and a skin external preparation and food containing the plant extract.

Background art

[0002] Causes of skin symptoms such as aging, UV rays, stress-induced skin wrinkles, spots, and skin elasticity reduction include dryness, decreased cellular function, melanin production and pigmentation due to UV rays, reduction and degeneration of dermal matrix components, Examples include oxidative damage of cells due to ultraviolet rays and the like. In order to prevent and improve such skin symptoms, various active ingredients have been searched and formulated. As the cell activator, the essence of Bonkan (see Patent Document 1), as the whitening agent, the water and / or organic solvent extract of Hakutsuru Reishi (see Patent Document 2), and as the antioxidant, the Salogase family Salogase An extract of a genus plant (see Patent Document 3) is known.

[0003] It should be noted that no prior art relating to a moisturizer, a cell activator, a whitening agent, and an antioxidant containing an extract of a plant of the genus Tochina is recognized.

Patent Document 1: Japanese Patent Laid-Open No. 2001-131045

Patent Document 2: Japanese Patent Laid-Open No. 2003-89630

Patent Document 3: Japanese Patent Laid-Open No. 10-182413

Disclosure of the invention

Problems to be solved by the invention

[0004] Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally-derived ingredients whose effects are not yet known, and the development of effective ingredients having excellent moisturizing action, cell activation action, whitening action or antioxidant action was expected. . The present invention has been made to find such an active ingredient, and is a humectant and a cell retentate that can be widely applied in the fields of external preparations for skin and foods and drinks. It aims at providing an active agent, a whitening agent, and an antioxidant.

Means for solving the problem

[0005] In order to find a moisturizer, a cell activator, a whitening agent, and an antioxidant that can be widely applied to fields such as external preparations for skin and foods and drinks, the present inventors have made various substances of natural origin. A study was conducted. As a result, the present inventors have found an excellent moisturizing action, cell activation action, whitening action and antioxidant action in extracts of plants belonging to the genus Tochinacea, and have further studied and completed the present invention. That is, the present invention provides a skin containing a moisturizer, a cell activator, a whitening agent, an antioxidant, and an extract of one or more plants of the plant belonging to the genus Tochina plant as an active ingredient. It provides external preparations and foods.

The invention's effect

[0006] According to the present invention, it is possible to provide a moisturizer, a cell activator, a whitening agent, and an antioxidant having excellent effects, and these can be blended in a composition such as a skin external preparation or food. As a result, various compositions can be provided that exhibit excellent effects in preventing and improving various skin symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumumi.

BEST MODE FOR CARRYING OUT THE INVENTION

[0007] Androsace plants (Androsace chamaeiasme SPP.), Andros ace umbellata, Androsace filiformis, Kitakozakura, which are plants used as raw materials of the present invention (Androsac e septentrionalis) and Androsace erecia are well known.

[0008] The plant used as a raw material for the present invention is not particularly limited as long as it is a plant belonging to the genus Tochinaiso, but for reasons such as availability and effectiveness, Tochinaiso (Andro sace chamaeiasme spp.) It is particularly preferable to use Androsace erecia, which is preferred to use Anarosace umbellata, Androsace, etc.

[0009] The extract of the plant belonging to the genus Tochinaisu in the present invention includes the active ingredient and the dried product of the plant belonging to the genus Tochinaisu, but it is preferable to use the extract extracted using various solvents. Any part of the leaves, flowers, seeds, roots, stems, buds, etc., is used for extraction. However, it is better to use whole grass for easy use. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to carry out the extraction after processing such as shredding, drying, and pulverization. Extraction can also be performed by an extraction method using a force immersed in an extraction solvent, a supercritical fluid, or a subcritical fluid. In order to increase the extraction efficiency, homogenization may be performed in stirring or an extraction solvent. The extraction temperature is suitably from about 5 ° C to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 to 14 days.

[0010] Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, ethyl ether, and propyl ether. Solvents such as ethers such as butyl acetate and esters such as butyl acetate and ketones such as acetone and ethylmethylolene ketone, and one or more of these may be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Further, one or more supercritical fluids or subcritical fluids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia may be used.

[0011] Extracts of the above-mentioned plants of the plant of the genus Tochina can be used as they are. Force concentrated and dried solids can be used again by dissolving them in water or a polar solvent, which impairs their physiological effects. It may be used after performing purification treatment such as decolorization, deodorization, desalting, etc., and fractionation treatment by column chromatography etc. within the range. The aforementioned extract of Tochina is also lyophilized after each treatment and fractionation and dissolved before use.

[0012] An extract of a plant belonging to the genus Tochinais has excellent moisturizing action, cell activation action, whitening action and antioxidant action, and should be used as a moisturizing agent, cell activator, whitening agent and antioxidant. Can do. In addition, moisturizers, cell activators, whitening agents, and antioxidants that contain extracts of the plants of the genus Tochina is useful for hair and can be taken orally, not just for topical use. It can also be applied to beverages or pharmaceuticals.

[0013] A moisturizing agent containing an extract of the plant of the genus Tochina is effective against skin and hair. The moisturizing effect is exerted, and the moisturizing effect on the skin is particularly high.

[0014] A cell activator comprising an extract of a plant belonging to the genus Tochina is effective for various cells, but particularly effective for dermal fibroblasts.

[0015] A whitening agent containing an extract of the plant of the genus Tochina is effective for improving pigmentation symptoms such as stains and freckles, and is particularly effective for suppressing melanin production.

[0016] An antioxidant comprising an extract of a plant belonging to the genus Tochina is effective as an active ingredient, but particularly exhibits an excellent free radical scavenging effect.

[0017] In addition, by incorporating an extract of the plant of the genus Tochina is used in an external preparation for skin, it has an excellent effect in preventing and improving skin symptoms such as wrinkles, tarmi, skin firmness, spots, Tasumi, dryness, and fine wrinkles. It can be used as a skin external preparation for moisturizing, a skin external preparation for whitening, or a skin external preparation for improving anti-aging. Furthermore, the extract of the plant of the genus Tochina can also be used for foods (including general foods and functional foods). The food of the present invention includes beverages. Functional food as used herein refers to foods taken for the purpose of maintaining health or providing nutrition instead of diet, such as health foods, nutritional supplements, supplements, and nutritional foods.

[0018] The amount of the extract of the plant belonging to the genus Tochina is mixed with the external preparation for skin can be adjusted depending on the type of external preparation for skin use and the purpose of use, etc. 0001-50. 0 weight ^_0/0 mosquitoes preferably 0. for <, is more preferably <(or, 0. 001-25. 0 by weight%.

[0019] The dosage form of the external preparation for skin containing the extract of the plant of the genus Tochina is arbitrary, and for example, it is provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. I can do it. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

[0020] It should be noted that the topical skin preparation containing the extract of the plant of the genus Tochina is, in addition to the extract of the plant of the genus Tochina, as required, usually a pharmaceutical product, quasi-drug, skin cosmetic, hair Oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilized in cosmetics and detergents Agents, cleaning agents, ultraviolet absorbers, thickeners, drugs, fragrances, resins, antibacterial / antifungal agents, alcohols, and the like can be appropriately blended. In addition, other moisturizers, cell activators, whitening agents, or antioxidants can be used in combination as long as the effects of the present invention are not impaired.

Example

[0021] In the following, production examples of extracts of the genus Tochina plant, tests for evaluating each action, formulation examples for skin external preparations and foods, and use tests will be described in more detail. The target range is not limited at all by this.

[0022] [Production Example 1]

10 liters of 50% by weight ethanol aqueous solution was added to 1 kg of dried pulverized product of Tochina, and soaked at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, an extract of the plant of the genus Tochina was obtained.

[0023] [Production Example 2]

9 liters of water was added to 1 kg of dry ground pulverized product of Tochina, and the mixture was extracted by refluxing at 90 ° C for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of the plant of the genus Tochina is obtained.

[0024] [Production Example 3]

Nine liters of methanol was added to 1 kg of dry ground pulverized Tochina, and soaked at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, an extract of the genus Tochinaiso was obtained.

[0025] [Production Example 4]

The plant was introduced into a supercritical extractor and extracted with a supercritical fluid of carbon dioxide at 40 ° C under a pressure of 15 MPa. The extract was collected to obtain an extract of the genus Tochinaiso.

[0026] First, a test for evaluating each action of a plant of the genus Tochina is described. In each test, the whole plant of Androssace elecia was extracted according to Production Example 1.

[0027] <Evaluation of cell activation in dermal fibroblasts>

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 4 cells per well. The seeding medium contains Dulbecco's modified One dull medium (DMEM) supplemented with 1% fetal bovine serum was used. After culturing for 24 hours, the medium was replaced with a test medium to which a sample of an arbitrary concentration was added, and further cultured for 48 hours. Next, replace the medium with 400 g / mL of 3- (4,5 dimethyl-2 thiazolyl) -2,5 diphenyltetrazolium bromide (MTT) and incubate for 2 hours to open the tetrazolium ring. The formazan produced by the extraction was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation action was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample taken as 100. In the table, ** indicates a significance probability of less than 1% (P <0.01) with respect to significance P value in t test.

[table 1]

[0029] As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the extract of the genus Tochinaiso.

[0030] <Evaluation of cell activation in epidermal cells>

The evaluation was performed according to the following procedure. Normal human epidermis cells were seeded in 96-well microplates at 2.0 × 10 ⁴ cells per well. Commercially available Hume dia-KG2 manufactured by Kurabo Industries was used as the seeding medium. After culturing for 24 hours, the medium was replaced with the test medium to which the sample was added, and further cultured for 24 hours. Subsequently, the medium containing 100 g / mL of 3- (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. And the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation action was evaluated by the difference between the two measured values. The evaluation results are shown in Table 2 as relative values when the cell activation effect when no sample is added is 100. [0031] [Table 2]

[0032] As is clear from Table 2, a significant epidermal cell activation effect was observed in the medium to which the extract of the plant of the genus Tochina was added.

[0033] <Evaluation of Tyrosinase Activity Inhibitory Action in Epidermal Melanocytes>

The evaluation was performed according to the following procedure. Normal human epidermal melanocytes manufactured by Kurabo Industries Co., Ltd. were seeded in a 96-well microplate so that there were 3.0 × 10 ⁴ cells per well. As a seeding medium, Mediuml 54S manufactured by Kurabo Industries was used. After 24 hours, the medium was replaced with a medium containing each concentration of sample, and the cells were further cultured for 48 hours. Next, the solution was replaced with 75 L of 1 wt% 1¾101-X-containing phosphate buffer to completely dissolve the cells, and 50 L was used as a crude enzyme solution. The crude enzyme solution was added with 50 li UDO. 05% by weight as a substrate and a phosphate buffer solution containing dopa, and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm was measured immediately after the addition of the substrate and at the end of the reaction with a microphone plate reader, and the amount of produced doonomelanin was calculated. Table 3 shows the tyrosinase activity inhibition rate of the sample as a relative value to the tyrosinase activity inhibition rate in the blank with no sample added.

[0034] [Table 3]

[0035] As is apparent from Table 3, when a medium supplemented with an extract of a Tochina plant was used, an excellent inhibitory effect on tyrosinase activity was observed.

[0036] <Evaluation of antioxidant effect by scavenging DPPH radicals>

The evaluation was performed according to the following procedure. 100 L of a solution of an extract of a Tochina genus plant diluted to an arbitrary concentration with a 50% by weight aqueous ethanol solution was added to a 96-well microplate. . Next, 100 L of 1,1-diphenyl-2-picrylhydrazinole (DPPH) solution prepared in ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left standing in a place with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank to which the sample was not added was (A) and the absorbance when the sample was added was (B), the value of equation (1) was the radial elimination rate. The evaluation results are shown in Table 4.

Formula (1) {1-(B) / (A)} X 100 (%)

[Table 4]

[0038] As is apparent from Table 4, it was found that the extract of the plant of the genus Tochinaiso has an antioxidant effect based on radical scavenging.

[0039] Subsequently, a formulation example of a topical skin preparation and a food containing the extract of the plant of the genus Tochina is related to the present invention.

[0040] [Prescription Example 1] Emulsion

10. 0 (% by weight)

Esang 4.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Polyoxyethylene monostearate

Sorbitan (20Ε · O.) 3

0

4. 0

(8) Methyl paraoxybenzoate 0.1

(9) Canolepoxy bininole polymer 0.15

(10) Purified water 57. 85

(Wt% aqueous solution) 20. 0 'Extract of genus plant [Production Example 1]

Production method: The oil phase components (1) to ½) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix evenly.

[Prescription Example 2] Lotion

(1) ethanol 15 - 0 (weight ^_0/0)

(2) Polyoxyethylene (40Ε · Ο.) Hardened castor oil 0 · 3

(3) Fragrance 0. 1

(4) Purified water 82. 38

(9) Extracts of Tochinaiso plant [Production Example 3]

Production method: Dissolve ( ² ) and (3) in (1). After dissolution, sequentially add ( ⁴ ) to (8), then stir well, add (9), and mix uniformly.

[Prescription Example 3] Cream

10. 0 (% by weight)

2. 0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

—Nore 3.6

½) Lipophilic type 2.0

10. 0

(8) Methyl paraoxybenzoate 0.1

(9) Arginine (20 wt% aqueous solution) 15. 0

(10) Purified water 40.7 (11) Carboxybule polymer (1 wt% aqueous solution) 15 · 0

(12) Tochinaiso plant extract [Production Example 1] 1.0

Production method: The oil phase components (1) to ½) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, add (11), start cooling, add (12) at 40 ° C and mix uniformly.

[Prescription Example 4] Essence

(1) Purified water 27-45 (wt%)

(2) Glycerin 14

(3) Sucrose fatty acid ester 1.3

(4) Carboxybule polymer (1 wt% aqueous solution) 17 · 5

(5) Sodium alginate (1 wt% aqueous solution) 15 · 0

(6) Polyglyceryl monolaurate 1

(7) Macadamia nut oil fatty acid phytosterinole 3.0

(8) N-lauroyl-L-glutamic acid

Di (Phytosteril 2—Otachidodecyl) 2 · 0

(9) Hardened palm oil 2.0

(10) Sukuran (Olive) 1 · 0

(11) Behenyl alcohol 0.75

(12) Beeswax 1 0

(13) Jojoba oil1.0

(14) 1, 3-butylene glycol 10 · 0

(15) L-Arginine (10 wt% aqueous solution) 2 · 0

(16) Extracts of plants belonging to the genus Tochina [Production Example 1] 1.0

Production method: The aqueous phase components (1) to ½) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Start cooling after emulsification and add (15) at 50 ° C. Cool to 40 ° C and add (16) to mix evenly. Match.

[0044] [Prescription Example 5] Aqueous:

(1) Canolepoxy bininole polymer 0.5 (wt%)

(2) Purified water 86 7

(3) Sodium hydroxide (10 wt% aqueous solution) 0 · 5

(4) Ethanore 10 · 0

(5) Methyl parabenzoate 0/1

(6) Fragrance 0 · 1

(7) Extracts of Tochinaiso plants [Production Example 4] 2.0

(8) Polyoxyethylene (60Ε · O.) Hydrogenated castor oil 0 · 1

Production method: Add (1) to (2), stir uniformly, and then add (3). After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, add (6) to (8) previously mixed and stir and mix uniformly.

[0045] [Prescription Example 6] Cleansing Fee

(1) Sukuwaran 81 and 0 (weight ^_0/0)

(2) Polyoxyethylene glyceryl isostearate 15

(3) Purified water 3

(4) Extracts of plants belonging to the genus Tochina [Production Example 4] 1.0

Production method: Dissolve (1) and (2) uniformly. Add (3) and (4) to this, and mix evenly.

[0046] [Prescription Example 7] face washing foam

16. 0 (wt%)

(2) Myristic acid 16.0

(3) Lipophilic type 2.0

20. 0

(5) Hydroxide 7.5

(6) Palm oil fatty acid 1.0

(7) Purified water 36.5

(8) Tochinaiso plant extract [Production Example 3] 1.0 Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C, and the oil phase components are mixed and stirred uniformly. Start cooling, add (8) at 40 ° C, and mix uniformly.

[0047] [Prescription Example 8] Makeup base cream

10. 0 (wt%)

Nore 2.0

(3) Glycerol tree 2-ethyl hexanoate 2.5

(4) Lipophilic type 0

: π

(6) Sucrose fatty acid 3

(7) Purified water 69.4

(8) Titanium oxide 0

(9) Bengala 0.

(10) Yellow iron oxide 0.4

(11) Fragrance 0. 1

'Extract of genus plant [Production Example 2] 1.2

Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, dissolved by heating at 75 ° C, and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C and mix evenly.

[0048] [Prescription Example 9] Emulsion:

Sun 2.0 (wt%)

5. 0

(3) Myristic acid; 5.0

One point 1. 0

(5) Polyoxyethylene (20Ε ・ O.)

Three 0. 7

(7) 1, 3-Butylene glycol 8.0

(8) Xanthan gum 0.1

(9) Methyl paraoxybenzoate 0.

(10) Purified water 57.4

(11) Acid 9.0

7. 4

(13) Bengala 0.5

(14) Yellow iron oxide

(15) Black iron oxide 0.1

(16) Fragrance 0. 1

'Extract of genus plant [Production Example 4] 1.0

Production method: The oil phase components (1) to ½) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C, and the pigments (11) to (15) are added to this and dispersed uniformly with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after emulsification, and components (16) and (17) are added sequentially at 40 ° C and mixed uniformly.

[Prescription Example 10] Water-in-oil emollient cream

(1) Current Roh 30.0 (weight ^_0/0)

2. 0

5. 0

(5) Sodium chloride 1.3

(6) Potassium chloride 0.1

): π—Nore 3.0

(8) 1, 3-butylene glycol 5.0

(9) Methyl paraoxybenzoate 0.1

(10) Extracts of Tochinais [Manufacturing Example 1] 1.0

(11) Purified water 47.4 (12) Fragrance

Manufacturing method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C while stirring. After mixing this, disperse uniformly into (1) to (3), which is heated and dissolved at 70 ° C. Add (7) to (10), which is dissolved in the remainder of (11) by heating at 70 ° C while stirring, and emulsify with a homomixer. Start cooling after emulsification, add (12) at 40 ° C, and mix evenly.

[0050] [Prescription Example 11] Pack

(1) Purified water 62.9 (wt%)

(2) Polybule alcohol 12.0

(3) Ethanol 17

(4) Glycerin 5.0

(5) Polyethylene glycol (average molecular weight 1000) 2 · 0

(6) Tochinaiso plant extract [Production Example 2] 1.0

(7) Fragrance 0. 1

Manufacturing method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and start cooling with stirring. Cool to 40 ° C, add (6) and (7), and mix evenly.

[0051] [Prescription Example 12] Bath salt

(1) Fragrance 0-3 (% by weight)

(2) Tochinaiso plant extract [Production Example 1] 1.0

(3) Sodium bicarbonate 50. 0

(4) Sodium sulfate 48.7

Production method: (1) to (4) are mixed uniformly.

[0052] [Prescription Example 13] Hair wax

3. 0 (% by weight)

(3) Cetyl alcohol

(4) Highly polymerized methylpolysiloxane -Sun 5.0 methylpolysiloxane copolymer 1.0

(7) Methyl parabenzoate 0/1

(8) 1,3-Butylene glycol 7.5

(9) Arginine 0-7

(10) Purified water 74

(11) Extracts of Tochinais [Manufacturing Example 1] 1.0

(12) Fragrance 0. 1

Production method: The oil phase components (1) to ½) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C, and the oil phase component is added and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C and mix evenly

[0053] [Prescription Example 14] Hair art nick

(1) Ethanol 50 - 0 (wt ^_0/0)

(2) Purified water 48 9

(3) Tochinaiso plant extract [Production Example 3] 1.0

(4) Fragrance 0 · 1

Production method: Components (1) to (4) are mixed and homogenized.

[0054] [Prescription Example 15] Tablet

(1) Extract of Tochinais [Manufacturing Example 1] 100. 0 (mg)

(2) Reduced maltose starch syrup 461.0

(4) Glycerin fatty acid ester 12.0

(5) Fragrance 12 0

Production method: (1) to (3) were respectively sieved and mixed, and then (4) to (5) were added and mixed. Thereafter, tableting was performed by a conventional method to obtain a tablet having a total amount of 600 mg.

[0055] Next, a use test was conducted using a formulation formulated with an extract of a plant of the genus Tochina, and dried. The improvement effect was evaluated for rough skin. At that time, the extract of the plant belonging to the genus Tochinaiso listed in Table 5 was added to the formulation of the emulsion shown in Formulation Example 1, and the use test was conducted as Examples 1-2. Moreover, the extract of the plant of the genus Tochina is replaced with purified water, and a use test was simultaneously conducted as Comparative Example 1.

[Table 5]

[0057] For each sample, a group of 20 female panelists with dry skin in their 30s to 50s who had markedly rough skin symptoms, and used blinds for 1 week to change the skin condition before and after use. Observed and evaluated. As an index of skin symptoms, rough skin due to dryness was evaluated in three stages: “Improved”, “Slightly improved”, and “No change”, and Table 6 shows the number of panelists that obtained each evaluation.

[0058] [Table 6]

[0059] From Table 6, it has become clear that the extract of the genus Tochinaiso has an excellent moisturizing effect.

Industrial applicability

[0060] The humectant, cell activator, whitening agent, and antioxidant of the present invention are useful for blending into skin external preparations, foods, pharmaceuticals, and quasi drugs. In addition, the extract of the plant of the genus Tochinacea according to the present invention may be a component derived from nature and has great significance as a skin external preparation and a food ingredient. Useful as food.

Claims

The scope of the claims

[1] A moisturizing agent containing as an active ingredient an extract of one or more plants selected from plants belonging to the genus Tochinaiso.

[2] A cell activator comprising, as an active ingredient, an extract of one or more plants selected from plants belonging to the genus Tochinaiso.

[3] A whitening agent comprising, as an active ingredient, an extract of one or more plants selected from plants belonging to the genus Tochinaiso.

[4] An antioxidant comprising, as an active ingredient, an extract of one or more plants selected from plants belonging to the genus Tochinaiso.

[5] An external preparation for skin containing an extract of one or more plants selected from plants belonging to the genus Tochinaiso.

[6] Food containing an extract of one or more plants selected from plants belonging to the genus Tochinaiso