WO2007125898A1 - Fermented neem fertilizer, fermented neem enzyme compost, fermented neem fertilizer pellet and process for producing them - Google Patents

Fermented neem fertilizer, fermented neem enzyme compost, fermented neem fertilizer pellet and process for producing them Download PDF

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Publication number
WO2007125898A1
WO2007125898A1 PCT/JP2007/058812 JP2007058812W WO2007125898A1 WO 2007125898 A1 WO2007125898 A1 WO 2007125898A1 JP 2007058812 W JP2007058812 W JP 2007058812W WO 2007125898 A1 WO2007125898 A1 WO 2007125898A1
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fermented
neem
fermentation
fertilizer
add
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PCT/JP2007/058812
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French (fr)
Japanese (ja)
Inventor
Toshihiko Sato
Yasuhito Narushima
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Kabushikikaisha Seikouen
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C05F5/008Waste from biochemical processing of material, e.g. fermentation, breweries
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/26Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Definitions

  • the present invention relates to a fermented neem fertilizer and a method for producing the same.
  • the fermented neem fertilizer of the present invention is obtained by fermenting neem or a processed product thereof using a fermentation base containing naturally-occurring carbohydrates, proteins and fats, and improves soil and repels pests, It has excellent effects such as increased permeability and degradability by enzymes, koji molds, natural yeast, lactic acid bacteria, etc. contained in fermented neem fertilizers, making it easier for plants to absorb nutrients in the soil.
  • Patent Document 1 soil improvement agents using natural ores
  • Patent Document 2 to 4 repellents and insecticides using natural neem
  • Patent Document 1 Japanese Patent Laid-Open No. 2005-281477
  • Patent Document 2 JP-A-2005-179328
  • Patent Document 3 JP 2004-277393 A
  • Patent Document 4 Japanese Patent Laid-Open No. 05-310523
  • Fermentation neem that has long-lasting crop damage to prevent soil damage caused by pesticides. It has long-lasting effects that prevent adverse effects on the human body and the environment.
  • a fertilizer and a method for producing the same are provided.
  • the present invention uses (I) a fermented neem fertilizer in which neem or a processed product thereof is fermented at 50 ° C. or less by adding koji mold together with the fermentation base, and (II) a fermentation base that has been previously fermented.
  • fermented neem fertilizer (III) first step of washing brown rice, steaming, adding koji mold and fermenting with primary sugar, second step of adding natural yeast 'lactic acid bacteria and secondary fermentation, A method for producing a fermented neem fertilizer, characterized in that it is produced by a third step in which neem or a processed product is added and subjected to tertiary fermentation, and a fourth step in which a primary sugar-koji fermentation product is further added and subjected to quaternary fermentation.
  • a method for producing fermented neem enzyme compost (V) a first step in which brown rice is washed and steamed and subjected to primary saccharification and fermentation, and a second step in which natural yeast and lactic acid bacteria are added to perform secondary fermentation. 10% by weight of natural organic matter, livestock manure, food residue, etc.
  • a method for producing fermented neem fertilizer pellets characterized by three steps and a fourth step in which the liquid obtained above is further diluted with an appropriate amount of water and kneaded into a pellet forming machine.
  • the neem used in the present invention refers to neem (Azadi rachta indica).
  • the leaf, xylem, root, or a pulverized product thereof neem oil (neem) Oil), neem oil extraction residue (dry neem), etc.
  • neem or a processed product thereof is fermented at 50 ° C or lower together with a fermentation substrate.
  • a fermentation substrate natural materials including starch, protein, lipids and the like are used.
  • rice, wheat, bark, corn, other minor grains beans such as soybeans, potatoes such as potatoes and sweet potatoes, okara, tea seeds, rapeseed oil cakes, soybean oil cakes, cashew nut shells, etc.
  • These fermentation substrates can be heated to high temperatures to gelatinize starch, hydrolyzed with acid or hydrolase, or pre-fermented with natural yeast lactic acid bacteria, etc.
  • the processed product can be added and fermented.
  • rods such as Aspergillus genus, Rhizopus genus, Mucor genus and the like, which have a function of degrading natural materials including starch, proteins, lipids, etc.
  • Aspergillus 'Soyer' is used.
  • natural fermentation mother, lactic acid bacteria, etc. can be used. Fermentation should be performed as much as possible by avoiding over 50 ° C and overheating, cooling, aeration, stirring, etc. to obtain a fermented fertilizer with excellent effectiveness. be able to.
  • the fermented product thus obtained is dried and pulverized to form a powdered and pelleted product.
  • the fermented product can be further fermented at 50 ° C or lower, such as by adding water or neem oil, to obtain a liquid product by removing the solid content.
  • the fermentation base is a component that is easily absorbed by plants by fermentation, and it is contained alive and accumulates in the soil due to the action of microorganisms and enzymes, causing damage. It will break down and absorb the undigested nutrients.
  • the odor peculiar to neem is alleviated, making it easier to apply, improving the soil, generating pests, and the effect lasts for a long time.
  • the components are as follows.
  • Example 2 The liquid material obtained in [Example 2] was placed in 54 parts of okara, 20 parts of rice bran, 5 parts of tea seeds and 20 parts of rice cake, and 20 to 45 ° C. Fermented for a day to obtain a cake-like fermented material.
  • Example 6 The liquid material obtained in [Example 2] was placed in 54 parts of okara, 20 parts of rice bran, 5 parts of tea seeds and 20 parts of rice cake, and 20 to 45 ° C. Fermented for a day to obtain a cake-like fermented material.
  • Example 6 Example 6
  • the liquid produced in [Example 2] is diluted 500 to 2000 times with water, and foliar application and soil irrigation are performed.
  • the cake-like fermented material produced in [Example 1] is soaked in soil at 20 to 30 kg per 10 ares.
  • the compost produced in [Example 4] is used as the original fertilizer and 50 kg per 10 ares is planted before planting. Furthermore, 10 to 20 kg per 10 ares is applied to the soil surface as additional fertilizer.
  • Pellet produced in [Example 3] is used as raw manure, and 50 kg per 10 ares is sown into the soil before planting. In addition, 10 to 20 kg per 10 ares is applied to the soil surface as additional fertilizer.
  • the control fertilizer is a dry cell fertilizer registered with the governor of Ibaraki Prefecture. After soil filling and fertilization, maintain for 3 weeks at constant moisture and temperature (5 ° C), sow and harvest after 3 weeks. Germination rate was 100% in both test and control groups.
  • a test sample of Fusarium oxysporum is inoculated in the middle of a potato glucose agar medium (pH 6.5) in which the liquid obtained in [Example 2] is diluted to a predetermined concentration in a Petri dish with a diameter of 9 cm, and 45 ° Keep in a sterile room of C for 7 days and calculate the inhibition rate relative to the untreated control.
  • Fusarium * oxysporum cultured on potato glucose agar medium (pH 6.5) for 6 days is cut with a special 6 mm diameter extruded pipe.
  • the suppression rate is based on the following formula (Equation 1).

Abstract

[PROBLEMS] To provide a fermented neem fertilizer that relating to avoiding of soil problems attributed to many years of continuous cropping, is free from any danger to human health and environment likely to occur at soil sterilization by pesticides and simultaneously realizes lasting activity, assured effect and pest repelling efficacy; and provide a process for producing the same. [MEANS FOR SOLVING PROBLEMS] Neem or matter obtained by treating the same together with a fermentation substrate is fermented at 50°C or below, thereby producing a fermented neem fertilizer. By using the fermented neem fertilizer, any soil problems can be avoided, and there can be attained suppression of pest breeding, easy use, friendliness to environment and healthy crop growth.

Description

明 細 書  Specification
発酵ニーム肥料並びに発酵ニーム酵素堆肥並びに発酵ニーム肥料ペレ ット並びにそれらの製造方法  Fermented Neem Fertilizer, Fermented Neem Enzyme Compost, Fermented Neem Fertilizer Pellet and Method for Producing them
技術分野  Technical field
[0001] 本発明は、発酵ニーム肥料及びその製造方法に関するものである。  [0001] The present invention relates to a fermented neem fertilizer and a method for producing the same.
[0002] 本発明の発酵ニーム肥料は、天然由来の炭水化物、たんぱく質、脂肪を含む発酵 基材を用いて、ニーム又はその処理物を発酵させて得られ、土壌改良と病害虫の忌 避効果や、発酵ニーム肥料に含まれる酵素や麹菌、天然酵母、乳酸菌等による浸透 性、分解性を増し、土壌中の栄養分を植物に吸収しやすくさせる等、優れた作用を 示す。 [0002] The fermented neem fertilizer of the present invention is obtained by fermenting neem or a processed product thereof using a fermentation base containing naturally-occurring carbohydrates, proteins and fats, and improves soil and repels pests, It has excellent effects such as increased permeability and degradability by enzymes, koji molds, natural yeast, lactic acid bacteria, etc. contained in fermented neem fertilizers, making it easier for plants to absorb nutrients in the soil.
背景技術  Background art
[0003] 従来の農業分野にお!、ては、長年の連作によって土壌障害が起き、これを回避す るために、農薬による土壌消毒が慣例として行われているが、強力な毒性のため、人 体や作物だけでなぐ環境にも悪影響を及ぼして 、る。  [0003] In the conventional agricultural field, soil damage has been caused by many years of continuous cropping, and in order to avoid this, soil disinfection with pesticides is customary, but because of its strong toxicity, It also has an adverse effect on the environment that goes beyond the human body and crops.
また、天然の鉱石を利用した土壌改良剤 (特許文献 1)や、また天然由来のニーム を利用した忌避剤や殺虫剤も提案されているが (特許文献 2〜4)、十分な効果が得 られていない。  In addition, soil improvement agents using natural ores (Patent Document 1) and repellents and insecticides using natural neem have been proposed (Patent Documents 2 to 4), but sufficient effects have been obtained. It is not done.
特許文献 1:特開 2005 - 281477号公報  Patent Document 1: Japanese Patent Laid-Open No. 2005-281477
特許文献 2 :特開 2005— 179328号公報  Patent Document 2: JP-A-2005-179328
特許文献 3:特開 2004— 277393号公報  Patent Document 3: JP 2004-277393 A
特許文献 4:特開平 05— 310523号公報  Patent Document 4: Japanese Patent Laid-Open No. 05-310523
発明の開示  Disclosure of the invention
発明が解決しょうとする課題  Problems to be solved by the invention
[0004] 長年の連作による土壌障害を回避するため行われている、農薬による土壌消毒の 人体や環境への悪影響がなぐ持続性があり、効果が確実で、病害虫の忌避効果も 併せ持つ、発酵ニーム肥料及びその製造方法を提供するものである。 [0004] Fermentation neem that has long-lasting crop damage to prevent soil damage caused by pesticides. It has long-lasting effects that prevent adverse effects on the human body and the environment. A fertilizer and a method for producing the same are provided.
課題を解決するための手段 [0005] 本発明の、ニームまたはその処理物を発酵基材と共に 50°C以下で発酵させた発 酵ニーム肥料を用いることにより、上記課題を解決することができるものである。 Means for solving the problem [0005] The above-described problems can be solved by using the fermented neem fertilizer obtained by fermenting neem or a processed product thereof together with a fermentation base material at 50 ° C or lower according to the present invention.
[0006] すなわち、本発明は、(I)ニームまたはその処理物を発酵基材と共に麹菌を加えて 50°C以下で発酵させた発酵ニーム肥料、 (II)あらかじめ発酵させた発酵基材を用い る (I)の発酵ニーム肥料、(III)玄米を洗米して蒸して麹菌を加えて一次糖ィヒ発酵させ る第一工程と、天然酵母 '乳酸菌付加し二次発酵させる第二工程と、ニームまたはそ の処理物を付加し三次発酵させる第三工程と、更に一次糖ィ匕発酵生産物を付加し 四次発酵させる第四工程とにより製造することを特徴とする発酵ニーム肥料の製造方 法、(IV)玄米を洗米して蒸して一次糖ィ匕発酵させる第一工程と、天然酵母'乳酸菌 付加し二次発酵させる第二工程と、ニームまたはその処理物を付加し三次発酵させ る第三工程と、更に第一工程で得られた発酵生産物を付加し四次発酵させる第四ェ 程と、これに、天然の有機物 ·畜産糞尿 ·食物残渣等を添加し ·更にサポニンを添カロ し、それらを混合して水分を調整し、一定期間堆積 '発酵'乾燥することを特徴とした 、発酵ニーム酵素堆肥の製造方法、(V)玄米を洗米して蒸して一次糖化発酵させる 第一工程と、天然酵母,乳酸菌付加し二次発酵させる第二工程と、得られた発酵体 に、天然の有機物 ·畜産糞尿 ·食物残渣等の水分を調整した物とそれの重量比で 10 [0006] That is, the present invention uses (I) a fermented neem fertilizer in which neem or a processed product thereof is fermented at 50 ° C. or less by adding koji mold together with the fermentation base, and (II) a fermentation base that has been previously fermented. (1) fermented neem fertilizer, (III) first step of washing brown rice, steaming, adding koji mold and fermenting with primary sugar, second step of adding natural yeast 'lactic acid bacteria and secondary fermentation, A method for producing a fermented neem fertilizer, characterized in that it is produced by a third step in which neem or a processed product is added and subjected to tertiary fermentation, and a fourth step in which a primary sugar-koji fermentation product is further added and subjected to quaternary fermentation. (IV) The first step of washing brown rice and steaming and fermenting the primary sugar and koji, the second step of adding natural yeast 'lactic acid bacteria and secondary fermentation, and adding the neem or its processed product and tertiary fermentation Add the fermented product obtained in the third step and the first step to add quaternary The fourth stage to be fermented, and natural organic matter, livestock manure, food residue, etc. are added to this.Additionally, saponin is added and mixed to adjust the water content, and then 'fermented' and dried for a certain period of time. A method for producing fermented neem enzyme compost, (V) a first step in which brown rice is washed and steamed and subjected to primary saccharification and fermentation, and a second step in which natural yeast and lactic acid bacteria are added to perform secondary fermentation. 10% by weight of natural organic matter, livestock manure, food residue, etc.
%の-一ム粕を添加'更にサポニンを添加し、それらを混合し一定期間堆積 '発酵' 乾燥することを特徴とした、発酵ニーム酵素堆肥の製造方法、(VI)玄米を洗米して 蒸して一次糖化発酵させる第一工程と、天然酵母 ·乳酸菌付加し二次発酵させる第 二工程と、得られた発酵体を濾過して固形分と液状分に分け、固形分に天然の有機 物'畜産糞尿'食物残渣等の水分を調整した物とそれの重量比で 10%のニーム粕を 混合した物に添加'更にサポニンを添加し、それらを混合し一定期間堆積 '発酵'乾 燥する第三工程と、更に上記で得られた液状体を適量の水で希釈し練り込みペレツ ト成型機にかけ粒状にする第四工程を特徴とした発酵ニーム肥料ペレットの製造方 法、である。 % Addition of -1 粕 粕 'Additional saponin, mix and mix for a period of time' Fermentation 'Drying method, fermented neem enzyme compost manufacturing method, (VI) Brown rice is washed and steamed The first step of primary saccharification and fermentation, the second step of adding natural yeast and lactic acid bacteria, and the second fermentation, and filtering the obtained fermented material into solid and liquid components. Livestock manure 'added to a mixture of food residues, etc. with adjusted water content and 10% neem meal by weight', add saponin, mix them, and deposit for a period of time 'fermentation' A method for producing fermented neem fertilizer pellets, characterized by three steps and a fourth step in which the liquid obtained above is further diluted with an appropriate amount of water and kneaded into a pellet forming machine.
[0007] 本発明で用いるニーム又はその処理物におけるニームとは、インドセンダン(Azadi rachta indica)をいい、本発明では、その葉部、木部、根部あるいはこれらの粉砕 物、ニーム実油(ニームオイル)、ニーム実油抽出残渣 (ドライニーム)等のいずれも 用!/、ることができる。 [0007] The neem used in the present invention refers to neem (Azadi rachta indica). In the present invention, the leaf, xylem, root, or a pulverized product thereof, neem oil (neem) Oil), neem oil extraction residue (dry neem), etc. You can!
[0008] 本発明においては、ニームまたはその処理物を発酵基材と共に 50°C以下で発酵さ せる。用いる発酵基材としては、でんぷん、たんぱく質、脂質等を含む天然材料を用 いる。  [0008] In the present invention, neem or a processed product thereof is fermented at 50 ° C or lower together with a fermentation substrate. As the fermentation base to be used, natural materials including starch, protein, lipids and the like are used.
例えば、米、麦、ヒェ、トウモロコシ、その他の雑穀類、大豆等の豆類、ジャガイモ、 サツマィモ等のィモ類、おから、茶の実粕、菜種油粕、大豆油粕、カシューナッツの 殻等が用いられる。これらの発酵基材は、あら力じめ高温に加熱してでんぷんを糊化 させ、酸あるいは加水分解酵素による加水分解や、天然酵母'乳酸菌等により予備 発酵させておいてもよぐこれにニーム又はその処理物を加えて発酵させることもでき る。  For example, rice, wheat, bark, corn, other minor grains, beans such as soybeans, potatoes such as potatoes and sweet potatoes, okara, tea seeds, rapeseed oil cakes, soybean oil cakes, cashew nut shells, etc. . These fermentation substrates can be heated to high temperatures to gelatinize starch, hydrolyzed with acid or hydrolase, or pre-fermented with natural yeast lactic acid bacteria, etc. Alternatively, the processed product can be added and fermented.
[0009] 発酵には、でんぷん、たんぱく質、脂質等を含む天然材料を分解する働きを持つ、 ァスペルギルス属、リゾプス属、ムコール属菌等の麹菌が繁用され、例えば、ァスぺ ルギルス 'ォリゼ、ァスペルギルス'ソーイエ等が用いられる。必要に応じて、天然酵 母、乳酸菌等を用いることもできる。発酵は、 50°C以上になるのを極力避けて、過熱 •冷却 ·通気や攪拌等により 50°C以下に保つように行うのがよぐこうすることにより効 果に優れた発酵肥料を得ることができる。  [0009] For fermentation, rods such as Aspergillus genus, Rhizopus genus, Mucor genus and the like, which have a function of degrading natural materials including starch, proteins, lipids, etc., are frequently used. Aspergillus 'Soyer' is used. If necessary, natural fermentation mother, lactic acid bacteria, etc. can be used. Fermentation should be performed as much as possible by avoiding over 50 ° C and overheating, cooling, aeration, stirring, etc. to obtain a fermented fertilizer with excellent effectiveness. be able to.
[0010] こうして得られた発酵生産物は、乾燥'粉砕'成型して、粉末状'ペレット状の製品と する。また、上記発酵生産物は、水やニームオイルをカ卩えるなどして、 50°C以下で更 に発酵させ、固形分を除いて液状の製品とすることもできる。本発明の発酵ニーム肥 料は、発酵基材が発酵により植物に吸収されやすい成分になっており、生きたまま含 まれて 、る微生物や酵素の作用により、土壌に蓄積して障害の原因になって 、る未 消化養分を分解'吸収させる。また、ニーム特有の臭いも緩和されていて施用しやす くなり、土壌を改良すると共に、病害虫が発生しに《なり、その効果も長期間持続す る。  [0010] The fermented product thus obtained is dried and pulverized to form a powdered and pelleted product. In addition, the fermented product can be further fermented at 50 ° C or lower, such as by adding water or neem oil, to obtain a liquid product by removing the solid content. In the fermented neem fertilizer of the present invention, the fermentation base is a component that is easily absorbed by plants by fermentation, and it is contained alive and accumulates in the soil due to the action of microorganisms and enzymes, causing damage. It will break down and absorb the undigested nutrients. In addition, the odor peculiar to neem is alleviated, making it easier to apply, improving the soil, generating pests, and the effect lasts for a long time.
[0011] これらは、直接または希釈して、土壌に施用してもよぐ一般肥料、畜産糞尿、食物 残渣、堆肥等と混ぜて使用することもできる。通常、 10アール当たり、固形製品は 10 〜500kg、好ましくは 50〜: LOOkgを用!ヽ、液状製品は 1〜20リツ卜ノレ、好ましくは 1〜 8リットルの割りで用いるのがよい。 発明を実施するための最良の形態 [0011] These can be used directly or diluted and mixed with general fertilizer, livestock manure, food residue, compost, etc. that can be applied to soil. Usually, 10 to 500 kg, preferably 50 to: LOOkg is used per 10 ares. LOOkg is used for liquid products, and 1 to 20 liters for liquid products, preferably 1 to 8 liters. BEST MODE FOR CARRYING OUT THE INVENTION
[0012] 本発明の最良の形態は、以下の実施例に述べるとおりである。  [0012] The best mode of the present invention is as described in the following examples.
実施例 1  Example 1
[0013] 玄米 1部を蒸して 3%ァスペルギルス'ソーイエ麹菌 0. 3部麹菌をカ卩えて混合したも の 7部に水 3部を加えて、 5〜7日間糖化発酵させて一次糖化発酵物を得る。おから 54部、米ぬか 20部、茶の実粕 5部及び-一ム粕 20部を混合し,この混合物に 3%ァ スペルギルス'ソーイエ麹菌 0. 3部を混合し、前記一次糖化発酵物の 2000倍液 0. 1部をカ卩えて混合する。混合物を 20〜45°Cに 20日間発酵させてケーキ状の発酵体 を得る。  [0013] 1 part brown rice steamed 3% Aspergillus soyella 0. 3 parts Aspergillus mixed and mixed 7 parts water 3 parts saccharification and fermentation for 5-7 days Get. Mix 54 parts of okara, 20 parts of rice bran, 5 parts of tea seeds and 20 parts of tea, and add 3 parts of 3% Aspergillus soyella to 3 parts of the mixture. 2000 times solution 0.1. The mixture is fermented to 20-45 ° C for 20 days to obtain a cake-like fermented material.
実施例 2  Example 2
[0014] 〔実施例 1〕で製造したケーキ状発酵体 3部を発酵槽に投入し、〔実施例 1〕で得られ た一次糖ィ匕発酵物の 2000倍液 0. 005部と水 7部を加えて混合し、空気を導入し、 且つ、 20〜45°Cに保ちながら 7日間撹拌する。これにニームオイル 1部を加え、〔実 施例 1〕で得られた一次糖ィ匕発酵物の 2000倍液 0. 005部を加えて、空気を導入し、 且つ、 20〜45°Cに保ちながら 7日間撹拌する。固形分を除いて、液状体を得る。 実施例 3  [0014] Three parts of the cake-like fermented product produced in [Example 1] was put into a fermentor, and a 2000-fold solution of the primary sugar-koji fermented product obtained in [Example 1] 0.005 part and water 7 Add part and mix, introduce air and stir for 7 days while keeping at 20-45 ° C. Add 1 part of neem oil to this, add 0.005 part of the 2000-fold solution of the primary sugar koji fermented product obtained in [Example 1], introduce air, and bring it to 20-45 ° C. Keep stirring for 7 days. The liquid is obtained by removing the solid content. Example 3
[0015] 〔実施例 1〕で製造したケーキ状発酵体 100部に、アミノ酸酵素液 (ライフメール社製 2000倍液) 0. 1部をカ卩えて混合し、ペレット成型機でペレット状に成型する。  [0015] 100 parts of the cake-like fermented material produced in [Example 1] is mixed with 0.1 part of an amino acid enzyme solution (Life Mail 2000-fold solution), and molded into a pellet using a pellet molding machine. To do.
成分は以下のとおりである。  The components are as follows.
水分 10. 7 %  Moisture 10.7%
pH5. 7 窒素全量 3. 22%  pH 5.7 Total nitrogen 3.22%
リン酸全量 4. 06%  Total amount of phosphoric acid 4.06%
カリ全量 1. 96%  Total potassium 1.96%
有機元素分析炭素率 14. 6 (CZN比)  Organic elemental analysis carbon ratio 14. 6 (CZN ratio)
実施例 4  Example 4
[0016] 〔実施例 1〕で製造した発酵体 3部を、天然の有機物,畜産糞尿,食物残渣等 7部に 混合し、〔実施例 2〕で獲られた液状体 2000倍液 0. 0005部を加えて、およそ 1力月 堆積 '発酵'乾燥させて堆肥を製造する。 [0016] 3 parts of the fermented product produced in [Example 1] was mixed with 7 parts of natural organic matter, livestock manure, food residue, etc., and the liquid obtained in [Example 2] 2000-fold solution 0.005 About 1 month Deposition 'fermented' dried to produce compost.
実施例 5  Example 5
[0017] 〔実施例 2〕で得られた液状体を、おから 54部、米ぬか 20部、茶の実粕 5部及び- ーム粕 20部にカ卩えて、 20〜45°Cに 20日間発酵させてケーキ状の発酵体を得る。 実施例 6  [0017] The liquid material obtained in [Example 2] was placed in 54 parts of okara, 20 parts of rice bran, 5 parts of tea seeds and 20 parts of rice cake, and 20 to 45 ° C. Fermented for a day to obtain a cake-like fermented material. Example 6
[0018] 〔実施例 5〕で得られたケーキ状発酵体 3部に、堆肥 7部を混合し、およそ 1力月堆 積'発酵 '乾燥させる。これに、〔実施例 2〕で得られた液状体 0. 1部を加えて、ペレツ ト成型機によりペレットとする。  [0018] 7 parts of compost is mixed with 3 parts of the cake-like fermented product obtained in [Example 5], and dried for about 1 month. To this, 0.1 part of the liquid obtained in [Example 2] is added and pelletized by a pellet molding machine.
[0019] <使用例 1 > [0019] <Usage example 1>
〔実施例 2〕で製造した液状体を 500〜2000倍に水で希釈し、葉面散布と土壌灌 水をする。  The liquid produced in [Example 2] is diluted 500 to 2000 times with water, and foliar application and soil irrigation are performed.
[0020] <使用例 2> [0020] <Usage example 2>
〔実施例 1〕で製造したケーキ状発酵体を、 10アール当たり 20〜30kgを土壌にす き込む。  The cake-like fermented material produced in [Example 1] is soaked in soil at 20 to 30 kg per 10 ares.
[0021] <使用例 3> [0021] <Usage example 3>
〔実施例 4〕で製造した堆肥を、元肥として、定植前に 10アール当たり 50kgをすき 込む。更に、追肥として 10アール当たり 10〜20kgを土壌の表面に施す。  The compost produced in [Example 4] is used as the original fertilizer and 50 kg per 10 ares is planted before planting. Furthermore, 10 to 20 kg per 10 ares is applied to the soil surface as additional fertilizer.
[0022] <使用例 4> [0022] <Usage example 4>
〔実施例 2〕で製造した液状体の 500〜 1000倍液を葉面散布する。  A 500 to 1000-fold solution of the liquid produced in [Example 2] is sprayed on the leaves.
[0023] <使用例 5> [0023] <Usage example 5>
〔実施例 3〕で製造したペレットを元肥として、定植前に 10アール当たり 50kgを土壌 にすき込む。また、追肥として、 10アール当たり 10〜20kgを土壌表面に施用する。  Pellet produced in [Example 3] is used as raw manure, and 50 kg per 10 ares is sown into the soil before planting. In addition, 10 to 20 kg per 10 ares is applied to the soil surface as additional fertilizer.
[0024] <試験例 1 > [0024] <Test Example 1>
〔実施例 3〕で得られるペレットの施肥による、コマツナの発芽並びに発芽後の生育 を観察する。対照肥料は、茨城県知事に登録済みの乾燥菌体肥料である。土壌充 填、施肥後 3週間一定の水分、温度 (5°C)条件で保った後、播種し、 3週間後収穫す る。発芽率は試験区,対照区共に 100%。  Observe the germination and growth after germination of Komatsuna by fertilizing the pellet obtained in [Example 3]. The control fertilizer is a dry cell fertilizer registered with the governor of Ibaraki Prefecture. After soil filling and fertilization, maintain for 3 weeks at constant moisture and temperature (5 ° C), sow and harvest after 3 weeks. Germination rate was 100% in both test and control groups.
[0025] [表 1] 供試肥料 対照肥料 [0025] [Table 1] Test fertilizer Control fertilizer
基準量区 124 103  Reference volume 124 103
2倍量区 128 109  Double volume 128 109
3倍量区 130 103  Triple volume 130 103
〔表 1〕 生体重指数  [Table 1] Raw body weight index
[0026] <試験例 2> <Test Example 2>
直径 9cmのペトリシャーレに〔実施例 2〕で得られる液状体を所定濃度に希釈したジ ャガイモグルコース寒天培地(pH6. 5)の真ん中に、フサリウム'ォキシスポラムのテ ストサンプルを接種し、 45°Cの無菌室内で 7日間保ち、無処理の対照区に対する抑 制率を求める。なお、テストサンプルは、フサリウム*ォキシスポラムをジャガイモグル コース寒天培地 (pH6. 5)に 6日間培養したものを、直径 6mmの専用押し出しパイ プでカットして用いる。  A test sample of Fusarium oxysporum is inoculated in the middle of a potato glucose agar medium (pH 6.5) in which the liquid obtained in [Example 2] is diluted to a predetermined concentration in a Petri dish with a diameter of 9 cm, and 45 ° Keep in a sterile room of C for 7 days and calculate the inhibition rate relative to the untreated control. For the test sample, Fusarium * oxysporum cultured on potato glucose agar medium (pH 6.5) for 6 days is cut with a special 6 mm diameter extruded pipe.
抑制率は、下記数式 (数 1)による。  The suppression rate is based on the following formula (Equation 1).
[0027] [数 1] [0027] [Equation 1]
· , 、 (無処理区の菌落直径■処理区の菌落直径) 抑制率(<½) = X 1 0 0 ·,, (Bacterial diameter of untreated area ■ Bacterial diameter of treated area) Inhibition rate (<½) = X 1 0 0
処理区の菌落直径  Bacteria fall diameter of treatment area
[0028] [表 2] [0028] [Table 2]
Figure imgf000007_0001
Figure imgf000007_0001
〔表 2〕 液状体濃度 (ppm)  [Table 2] Liquid concentration (ppm)
<試験例 3 > <Test Example 3>
試験例 2と同様にして、〔実施例 3〕で得られるペレットの抑制率(%)を求める。 結果は次のとおりである。 [0030] [表 3] In the same manner as in Test Example 2, the inhibition rate (%) of the pellet obtained in [Example 3] is obtained. The results are as follows. [0030] [Table 3]
Figure imgf000008_0002
Figure imgf000008_0002
〔表 3〕 ペレッ ト濃度 (ppm)  [Table 3] Pellet concentration (ppm)
[0031] <試験例 4 > [0031] <Test Example 4>
直径 9cmのペトリシャーレに実施例 6で得られるペレットを 4000ppmに希釈添カロし たジャガイモグルコース寒天培地(pH6. 5)の真ん中に、フサリウム 'ォキシスポラム( Fuarium oxysporum )、リゾクトニア'ソラニイ (Rhijoctonia solani)、スクレ口チウム '口ノレ フシィ (Sclerotium rolfsii)及びコレトリクム ·グロースポリオイデス (Colletrichum glooes porioides)のテストサンプルを接種し、 45°Cの無菌室内で 7日間保ち、無処理の対照 区に対する抑制率を求める。なお、テストサンプルは、試験例 2に準じて製造する。 抑制率は、下記数式 (数 2)による。  In the middle of a potato glucose agar medium (pH 6.5) obtained by diluting the pellet obtained in Example 6 to 4000 ppm in a Petri dish with a diameter of 9 cm, Fusarium oxysporum, Rhijoctonia solani, Inoculate the test sample of Sclerotium rolfsii and Colletrichum glooes porioides and keep in a sterile room at 45 ° C for 7 days, and calculate the inhibition rate against the untreated control. . Test samples shall be manufactured according to Test Example 2. The suppression rate is based on the following formula (Equation 2).
[0032] [数 2] [0032] [Equation 2]
· , 、 (無処理区の菌落直径'処理区の菌落直径) ·,, (Bacterial diameter of untreated area 'Bacteria diameter of treated area)
抑制率(《½) = X 1 0 0  Suppression rate (<< ½) = X 1 0 0
処理区の菌落直径  Bacteria fall diameter of treatment area
[0033] [表 4] [0033] [Table 4]
Figure imgf000008_0001
Figure imgf000008_0001

Claims

請求の範囲 The scope of the claims
[1] ニームまたはその処理物を発酵基材と共に 50°C以下で発酵させた発酵ニーム肥 料。  [1] Fermented neem fertilizer obtained by fermenting neem or a processed product thereof with a fermentation substrate at 50 ° C or lower.
[2] あらかじめ発酵させた発酵基材を用いる請求項 1の発酵ニーム肥料。  [2] The fermented neem fertilizer according to claim 1, which uses a fermentation base previously fermented.
[3] 玄米を洗米して蒸して一次糖ィ匕発酵させる第一工程と、天然酵母 '乳酸菌付加し 二次発酵させる第二工程と、ニームまたはその処理物を付加し三次発酵させる第三 工程と、更に第一工程で得られた発酵生産物を付加し四次発酵させる第四工程とに より製造することを特徴とする発酵ニーム肥料の製造方法。 [3] The first step of washing brown rice and steaming it to ferment primary sugar and koji, the second step of adding natural yeast 'lactic acid bacteria and secondary fermentation, and the third step of adding neem or its processed products and tertiary fermentation And a fourth step of adding a fermentation product obtained in the first step and performing a fourth fermentation, and a method for producing a fermented neem fertilizer.
[4] 玄米を洗米して蒸して一次糖ィ匕発酵させる第一工程と、天然酵母 '乳酸菌付加し 二次発酵させる第二工程と、ニームまたはその処理物を付加し三次発酵させる第三 工程と、更に第一工程で得られた発酵生産物を付加し四次発酵させる第四工程と、 これに、天然の有機物 ·畜産糞尿 ·食物残渣等を添加し ·更にサポニンを添加し、そ れらを混合して水分を調整し、一定期間堆積 '発酵'乾燥することを特徴とした、発酵 ニーム酵素堆肥の製造方法。 [4] The first step of washing brown rice, steaming and fermenting primary sugar and koji, the second step of adding natural yeast 'lactic acid bacteria and secondary fermentation, and the third step of adding neem or its processed product and tertiary fermentation In addition, add the fermentation product obtained in the first step and perform the fourth fermentation, and add natural organic matter, livestock manure, food residue, etc. to this, add saponin, and A method for producing fermented neem enzyme compost, characterized in that the water content is adjusted by mixing them, and the sedimentation 'fermentation' is dried for a certain period of time.
[5] 玄米を洗米して蒸して一次糖ィ匕発酵させる第一工程と、天然酵母 '乳酸菌付加し 二次発酵させる第二工程と、得られた発酵体に、天然の有機物 ·畜産糞尿 ·食物残 渣等の水分を調整した物とそれの重量比で 10%の-一ム粕を添加'更にサポニンを 添加し、それらを混合し一定期間堆積'発酵 ·乾燥することを特徴とした、発酵ニーム 酵素堆肥の製造方法。  [5] First step to wash brown rice, steam and ferment primary sugar and koji, second step to add secondary fermentation with natural yeast 'lactic acid bacteria, and natural fermented manure to live fertilizer It is characterized by adding water with adjusted water content such as food residue and 10% by weight of it-added saponin, adding saponin, mixing them, depositing for a certain period of time, fermentation and drying, Fermentation Neem A method for producing enzyme compost.
[6] 玄米を洗米して蒸して一次糖ィ匕発酵させる第一工程と、天然酵母 '乳酸菌付加し 二次発酵させる第二工程と、得られた発酵体を濾過して固形分と液状分に分け、固 形分に天然の有機物 ·畜産糞尿'食物残渣等の水分を調整した物とそれの重量比で 10%の-一ム粕を混合した物に添加'更にサポニンを添加しそれらを混合し一定期 間堆積 '発酵'乾燥する第三工程と、更に上記で得られた液状体を適量の水で希釈 し練り込みペレット成型機にかけ粒状にする第四工程を特徴とした発酵ニーム肥料 ペレットの製造方法。  [6] Brown rice is washed and steamed and fermented to primary sugar and koji fermentation, natural yeast 'second process to add lactic acid bacteria and secondary fermentation, and the resulting fermented material is filtered to obtain solids and liquids Add to a mixture of natural organic matter, livestock manure and water with adjusted water content such as food residue and 10% by weight of the mixture, and add saponin. A fermented neem fertilizer characterized by a third step of mixing and depositing for a period of time 'fermentation' and a fourth step of further diluting the liquid obtained above with a suitable amount of water and kneading it into a pellet molding machine. Pellet manufacturing method.
PCT/JP2007/058812 2006-04-27 2007-04-24 Fermented neem fertilizer, fermented neem enzyme compost, fermented neem fertilizer pellet and process for producing them WO2007125898A1 (en)

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WO2010113255A1 (en) * 2009-03-31 2010-10-07 株式会社コア・タムタム Method of producing fermented neem
JP2010270086A (en) * 2009-05-25 2010-12-02 Shugo Hama Composition for disease and insect damage-controlling agent, spray agent produced by diluting the same, and method of producing the same
CN103193534A (en) * 2013-04-02 2013-07-10 东北农业大学 Production technology of dry raising strong rice seedling biological matrix
JP2014015424A (en) * 2012-07-10 2014-01-30 Kanzai-Net Co Ltd Growth environment improvement material of animal and plant, and method of application of growth environment improvement material
CN105960876A (en) * 2016-04-27 2016-09-28 安徽乙地生态科技有限公司 Method for alleviating fertilizer-based continuous-cropping
CN107046839A (en) * 2016-12-22 2017-08-18 阜阳市殿兴农业科技有限公司 A kind of strawberry continuous cropping obstruction recovery method
CN109022298A (en) * 2018-07-31 2018-12-18 上海潮庆生物科技有限公司 A kind of unartificial yeast and preparation method thereof prepared using fruits and vegetables as fermenting raw materials
CN112830826A (en) * 2019-11-22 2021-05-25 湖南启农生物科技有限公司 Camellia oleifera shell organic fertilizer and preparation method thereof

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Publication number Priority date Publication date Assignee Title
WO2010113255A1 (en) * 2009-03-31 2010-10-07 株式会社コア・タムタム Method of producing fermented neem
JP2010270086A (en) * 2009-05-25 2010-12-02 Shugo Hama Composition for disease and insect damage-controlling agent, spray agent produced by diluting the same, and method of producing the same
JP2014015424A (en) * 2012-07-10 2014-01-30 Kanzai-Net Co Ltd Growth environment improvement material of animal and plant, and method of application of growth environment improvement material
CN103193534A (en) * 2013-04-02 2013-07-10 东北农业大学 Production technology of dry raising strong rice seedling biological matrix
CN105960876A (en) * 2016-04-27 2016-09-28 安徽乙地生态科技有限公司 Method for alleviating fertilizer-based continuous-cropping
CN107046839A (en) * 2016-12-22 2017-08-18 阜阳市殿兴农业科技有限公司 A kind of strawberry continuous cropping obstruction recovery method
CN109022298A (en) * 2018-07-31 2018-12-18 上海潮庆生物科技有限公司 A kind of unartificial yeast and preparation method thereof prepared using fruits and vegetables as fermenting raw materials
CN112830826A (en) * 2019-11-22 2021-05-25 湖南启农生物科技有限公司 Camellia oleifera shell organic fertilizer and preparation method thereof

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