Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
  • A23F5/00—Coffee; Coffee substitutes; Preparations thereof
  • A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
  • A23F5/243—Liquid, semi-liquid or non-dried semi-solid coffee extract preparations; Coffee gels; Liquid coffee in solid capsules
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
  • A23C21/00—Whey; Whey preparations
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
  • A23F5/00—Coffee; Coffee substitutes; Preparations thereof
  • A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
  • A23F5/36—Further treatment of dried coffee extract; Preparations produced thereby, e.g. instant coffee
  • A23F5/40—Further treatment of dried coffee extract; Preparations produced thereby, e.g. instant coffee using organic additives, e.g. milk, sugar
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
  • A23G1/00—Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
  • A23G1/30—Cocoa products, e.g. chocolate; Substitutes therefor
  • A23G1/56—Liquid products; Solid products in the form of powders, flakes or granules for making liquid products, e.g. for making chocolate milk, drinks and the products for their preparation, pastes for spreading or milk crumb
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/04—Animal proteins
  • A23J3/08—Dairy proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
  • A23L2/385—Concentrates of non-alcoholic beverages
  • A23L2/39—Dry compositions
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
  • A23L2/52—Adding ingredients
  • A23L2/66—Proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L23/00—Soups; Sauces; Preparation or treatment thereof
  • A23L23/10—Soup concentrates, e.g. powders or cakes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
  • A23L33/17—Amino acids, peptides or proteins
  • A23L33/19—Dairy proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

• the present invention relates to whey protein micelles, particularly to a method and compositions for forming them in situ.
• the present invention also pertains to instant beverages or liquid comestibles containing said micelles.
• Protein constitutes an indispensable part of the diets of many people. It is not only used for its nutritional value but also imparts desirable texture and stabilisation to foods. For instance, in fat-containing products, the fat must remain stabilized over the entire shelf life of the product, so that no phase separation occurs.
• emulsifying agents are utilized, that provide a stabilization of the emulsion once formed, based on their inherent property of a lipophilic or hydrophobic part being soluble in the non-aqueous phase and a polar or hydrophilic part being soluble in water such that said molecules facilitate emulsifying one phase in the other phase.
• the emulsifying agents also protect the once formed droplets from aggregation and coalescence.
• emulsifying agents naturally occurring substances are used, such as hydrocolloids, phospholipids (lecithin) or glycolipids and on the other hand synthetic agents like stearyl-2-lactylate or mono-, diacylglycerides, etc. may also be used.
• One of the major drawbacks of the agents resides in that they sometimes substantially add to the costs of the final product, and do not add to the nutritional value of the product. Sometimes, such kinds of materials also do not show adequate stabilising properties because of an interfacial competition with proteins.
• protein is also being used as an emulsifier and as a partial substitute for fat.
• US 6767575 Bl discloses a preparation of an aggregate whey protein product, whereby whey protein is denatured by acidification and heating. The protein aggregates thus obtained are used in food application.
• GB 1079604 describes improvements in the manufacture of cheese, whereby whey proteins undergo heat treatment at an optimum pH value, in order to obtain insoluble whey proteins which are then added to raw milk.
• WO 93/07761 is concerned with the provision of a dry microparticulated protein product which can be used as a fat substitute.
• US 5750183 discloses a process for producing proteinaceous microparticles which are useful as fat substitute containing no fat.
• a proteinaceous fat substitute is also disclosed in WO 91/17665 whereby the proteins are in the form of a water- dispersible microparticulated denatured whey protein. Apart from the food applications, proteins are also present in many pharmaceutical and cosmetic compositions.
• This gel effect presents limitation in terms of not only processability (e.g. clogging of machines used in the manufacture of protein-containing products) but also in terms of the texture thus obtained, which may not be desirable for the wide range of protein applications.
• Controlled denaturation of proteins is thus desirable in order to widen the use of proteins.
• EP 0603981 also describes a heat stable oil-in-water emulsion containing proteins.
• micellar whey protein by heat treating a hydrolysed whey protein solution.
• the micellar whey protein are characterised by an irregular shape .
• an object of the invention is to improve the usability and applicability of proteins in heating processes.
• a method for preparing a hot beverage or liquid comestible comprising the following steps of providing a beverage or liquid comestible package comprising native whey protein and heating said package in order to prepare a heated ready-to- eat beverage or liquid comestible and at the same time transforming the native whey protein at least partially into whey protein micelles.
• the invention relates to the food or drink composition thus obtained.
• the invention also provides, in a third aspect, an instant food package comprising whey protein and beverage or liquid comestible ingredients.
• Fig. 1 shows the result of an experiment demonstrating the effect of pH and heat treatment on the micellisation of ⁇ - lactoglobulin .
• Fig. 2 is showing a mean to determine the pH of micellisation for a commercial preparation (Bipro®, Batch JE032-1-420) using turbidity measurements at 500 nm.
• Fig. 3 is a Transmission Electron Microscopy micrograph from whey protein micelles (2 wt.%, WPI 95, Lactalis) at pH 7.4. Scale bar is 200 nm.
• Fig. 4 shows the result of an experiment evaluating the impact of the ionic strength (Arginine HCl) on the formation of protein micelles at constant pH of 7.0.
• Fig. 5 shows the volume stability (FVS) of foam stabilized by 1 wt . % ⁇ -lactoglobulin micelles
• Fig. 6 shows the intensity-based equivalent hydrodynamic diameter of whey protein obtained by heat-treatment of a lwt% ⁇ -lactoglobulin dispersion for 15 min at 85°C at pH ranging from 2 to 8.
• Whey protein micelles are obtained at pH 4.25 (positively charged with a zeta potential around +25mV) and at pH 6.0 (negatively charged with a zeta potential around -3OmV) .
• Z-averaged hydrodynamic diameter of the micelles was 229.3 nm at pH 4.25 and 227.2 nm at pH 6.0.
• the corresponding micrographs of the micelles obtained by TEM after negative staining are shown. Scale bars are 1 ⁇ m.
• Fig. 7 shows a highly schematic structure of a whey protein micelle.
• Fig. 8 is a negative staining TEM micrograph of a whey protein micelles dispersion obtained at 4% protein content.
• Fig. 9 is a negative staining TEM micrograph of a whey protein micelle dispersion obtained at 20% protein content after microfiltration .
• Fig. 10 shows the heat stability of a whey protein micelle dispersion obtained at 10% protein content after microfiltration at pH 7.0 in presence of NaCl after heating at 85°C for 15 min .
• Fig. 11 shows the heat stability of a whey protein dispersion obtained at 4% protein content at pH 7.0 in presence of NaCl after heating at 85°C for 15 min.
• Fig. 12 is a graph showing the size distribution of micelles obtained by the process of the invention using a 4% Prolacta 90 whey protein isolate treated at pH 5.9.
• a method for preparing a hot beverage or liquid comestible whereby the native whey proteins present in a package prior to heating are transformed at least partially into whey protein micelles.
• Figure 7 is a schematic representation of the micelles obtained by the method of the present invention, wherein the whey proteins are arranged in such a way that the hydrophilic parts of the proteins are oriented towards the outer part of the agglomerate and the hydrophobic parts of the proteins are oriented towards the inner "core" of the micelle.
• This energetically favourable configuration offers good stability to these structures in a hydrophilic environment .
• the specific micelle structure can be seen from the figures, in particular figures 3, 8, 9, wherein the micelles consist essentially of spherical agglomerates of denatured whey protein.
• the micelles of the present invention are particularly characterised by their regular, spherical shape.
• hydrophobic phase e.g. a fat droplet or air
• hydrophilic phase e.g. a hydrophilic phase
• the micelles produced by the method of the present invention have an extremely sharp size distribution (see Fig. 12), such that more than 80% of the micelles produced will have a size smaller than 1 micron, preferably between lOOnm and 900nm, more preferably between 100-770nm, most preferably between 200 and 400nm.
• the mean diameter of the micelles can be determined using Transmission Electron Microscopy (TEM) .
• TEM Transmission Electron Microscopy
• the liquid micelle samples are encapsulated in agar gel tubes. Fixation is achieved by immersion in a solution of 2.5% glutaraldehyde in 0. IM, pH 7.4 cacodylate buffer and post-fixation with 2% Osmium tetroxide in the same buffer, both solutions containing 0.04% Ruthenium red. After dehydration in a graded ethanol series (70, 80, 90, 96, 100% ethanol) , the samples are embedded in Spurr resin (Spurr/ethanol 1:1, 2:1, 100%).
• the micelles described above may be formed in situ according to the present invention and are present in the food or drink composition obtainable by the method of the present invention.
• the first step in the method of the present invention is to provide a beverage or liquid comestible package comprising native whey protein.
• any commercially available whey protein isolates or concentrates may be used, i.e. whey protein obtained by any process for the preparation of whey protein known in the art, as well as whey protein fractions prepared therefrom or proteins such as ⁇ -lactoglobulin (BLG) , ⁇ -lactalbumin and serum albumin.
• whey protein obtained by any process for the preparation of whey protein known in the art, as well as whey protein fractions prepared therefrom or proteins such as ⁇ -lactoglobulin (BLG) , ⁇ -lactalbumin and serum albumin.
• sweet whey obtained as a by-product in cheese manufacture acid whey obtained as by ⁇ product in acid casein manufacture
• native whey obtained by milk microfiltration or rennet whey obtained as by-product in rennet casein manufacture may be used as the whey protein.
• the whey protein may be from a single source or from mixtures of any sources.
• the whey protein does not undergo any hydrolysis step prior to micelle formation.
• the whey protein is not subjected to any enzymatic treatment prior to micellisation .
• the present invention is not restricted to whey isolates from bovine origin, but pertains to whey isolates from all mammalian animal species, such as from sheep, goats, horses, and camels.
• the process according to the present invention applies to mineralised, demineralised or slightly mineralised whey preparations.
• lightly mineralized is meant any whey preparation after elimination of free minerals which are dialyzable or diafiltrable, but which maintains minerals associated to it by natural mineralisation after preparation of the whey protein concentrate or isolate, for example.
• These "slightly mineralised” whey preparations have had no specific mineral enrichment.
• Whey proteins are an excellent source of essential amino acids (AA) (45%). Compared to casein (containing 0.3g cysteine/100g protein), sweet whey proteins contain 7 times more cysteine, and acid whey 10 times more cysteine. Cysteine is the rate limiting amino acid for glutathione (GSH) synthesis, a tripeptide made of glutamate cysteine and glycine which has primary important functions in the defence of the body in case of stress. Requirements in these amino acids may be increased in case of stress and in elderly people. Also, glutathione oral supplementation with whey protein has been shown to increase plasma GSH levels of HIV-infected patients (Eur. J. Clin. Invest. 2001; 31, 171-178) .
• GSH glutathione
• whey proteins include enhancement of muscle development and building, as well as muscle maintenance in children, adults or elderly people, enhancement of the immune function, improvement of cognitive function, control of blood glucose such that they are suitable for diabetics, weight management and satiety, anti-inflammatory effects, wound healing and skin repair, lowering of the blood pressure, etc.
• PER 118
• PER 100
• PER a measure of a protein quality assessed by determining how well such protein supports weight gain. It can be calculated by the following formula:
• PER body weight growth (g) / protein weight intake (g) .
• native whey proteins are provided in a package.
• the contents of the package may be in the form of dry ingredients to be diluted or in a liquid form.
• the content of the package is preferably an essentially dry powder.
• Said powder comprises native whey protein in an amount of at least 4%, preferably no more than 6%.
• the powder may comprise further food ingredients in powder form, such as dehydrated culinary, salts, dry soluble coffee granules, tea extracts, plant extracts, sugars etc.
• the dry package contents Prior to heating, the dry package contents are diluted such that when in solution the whey proteins are present in an amount of 0.1 wt . % to 12 wt.%, preferably in an amount of
• 0.1 wt.% to 8 wt.% more preferably in an amount of 0.2 wt.% to 7 wt.%, even more preferably in an amount of 0.5 wt.% to 6 wt.%, most preferably in an amount of 1 wt.% to 4 wt.% on the basis of the total weight of the solution.
• the powder is preferably diluted with water.
• the liquid comprises native whey protein in an amount of 0.1 wt.% to 12 wt.%, preferably in an amount of 0.1 wt.% to 8 wt.%, more preferably in an amount of 0.2 wt.% to 7 wt.%, even more preferably in an amount of 0.5 wt.% to 6 wt.%, most preferably in an amount of 1 wt . % to 4 wt.% on the basis of the total weight of the solution.
• the aqueous solution of the whey protein preparation as present before the heating step may also comprise additional compounds, which may stem from the powder ingredients or from the solution itself. Such additional compounds are for example by-products of the respective whey production processes, other proteins, gums or carbohydrates .
• the package may comprise other food ingredients such as soup ingredients, sauce ingredients, cocoa ingredients, tea ingredients, plant extracts, sweet dessert ingredients, fats, salts, emulsifiers, sugars, maltodextrins, polysaccharides such as acacia gum or carrageenan, cereals, soluble fibres etc. It may comprise aromas such as vanilla, caramel, fruit, chocolate, coffee, cinnamon aroma etc. It may also comprise further functional ingredients such as sweeteners, caffeine, vitamins, minerals, drugs, ligands, bioactive agents, etc.
• the whey protein, as well as the fractions and/or the main proteins thereof may be used in purified form or likewise in form of a crude product.
• the content of divalent cations in the whey protein for the preparation of the beverage or liquid comestible may be less than 2.5%, more preferably less than 2%, even more preferably less than 0.2%.
• Most preferably the whey proteins are completely demineralised.
• the pH and the ionic strength of the aqueous native whey protein solution are important factors in the present method.
• native whey proteins which are virtually devoid or depleted of free cations such as Ca, K, Na, Mg
• whey proteins micelles having a diameter of less than l ⁇ m be obtained. These micelles will have an overall negative charge.
• the same micelle form can also be obtained symmetrically below the isoelectrical pH, i.e from 3.5 to 5.0, more preferably 3.8 to 4.5, resulting in micelles being positively charged (see Figure 6) .
• micellisation of whey proteins may be done in a salt free solution at a pH value adjusted between 3.8 and 4.5 depending on the mineral content of the protein source.
• the micelles obtained by the method of the present invention preferably have an overall negative charge.
• the pH of the aqueous solution prior to heating may be in a range of from 5 to 9.
• the pH may be in the range of from 5.6 to 6.4, more preferably from 5.8 to 6.0 for a low divalent cation content (e.g. less than 0.2% of the initial whey protein powder) .
• the pH is increased up to 8.4 depending on the mineral content of whey protein source (concentrate or isolate) .
• the pH may be between 7.5 to 8.4, preferably 7.6 to 8.0 to obtain negatively charged micelles in the presence of large amounts of free minerals and the pH may be between 6.4 to 7.4, preferably 6.6 to 7.2 to obtain negatively charged micelles in the presence of moderate amounts of free minerals.
• the higher the calcium and/or magnesium content of the initial whey protein powder the higher the pH of micellisation.
• demineralise In order to standardize the conditions of formation of the whey protein micelles, it is most preferable to demineralise by any of the known demineralisation techniques (dialysis, ultrafiltration, reverse osmosis, ion exchange chromatography...) , any source of liquid native whey proteins with a protein concentration ranging from that of sweet whey, microfiltration permeate of milk or acid whey
• the dialysis can be done against water
• the pH of whey protein micelle formation is below pH 7.0, more preferably comprised between 5.8 to 6.6.
• the pH is generally adjusted by the addition of acid, which is preferably food grade, such as e.g. hydrochloric acid, phosphoric acid, acetic acid, citric acid, gluconic acid or lactic acid.
• acid which is preferably food grade, such as e.g. hydrochloric acid, phosphoric acid, acetic acid, citric acid, gluconic acid or lactic acid.
• alkaline solution which is preferably food grade, such as sodium hydroxide, potassium hydroxide or ammonium hydroxide.
• the pH of the liquid is between 5 and 9, preferably between 5 and 8.
• the dry ingredients are selected such that when diluted with water, the pH of the solution prior to heating is between 5 and 9, preferably between 5 and 8.
• ionic strength may be adjusted by organic or inorganic ions in such a way that allows micellisation at a constant pH value of 7.
• Figure 4 shows that micelles may be formed at a constant pH value of 7.0 while the ionic strength is varied by the addition of 70-80 mM of arginine HCl.
• a buffer may be further added to the aqueous solution of whey protein or to the dry powder so as to avoid a substantial change of the pH value during heat treatment of the whey protein.
• the buffer may be selected from any food-grade buffer system, i.e. acetic acid and its salts, such as e.g. sodium acetate or potassium acetate, phosphoric acid and salts thereof, e.g. NaH 2 PO 4 , Na 2 HPO 4 ,
• KH 2 PO 4 K 2 HPO 4
• Adjusting the pH and/or the ionic strength of the aqueous solution results in a controlled process yielding micelles having a size between 100nm-900nm, preferably between 100- 700nm, most preferably between 200-400nm.
• the distribution of micelles having dimensions between 100- 700nm is greater than 80% when carrying out the process of the invention (see Figure 12) .
• the whey protein does not undergo any hydrolysation step prior to micelle formation.
• the preparation comprising native whey protein is then subjected to the heat treatment.
• the package of the present invention is in the form of a powder, water is generally added prior to the heating step.
• the temperature in the range of from about 70 to below 95 °C, preferably of from about 82 to about 89 0 C, more preferably of from about 84 to about
• the desired temperature is kept at this temperature for a minimum of 10 seconds and a maximum of 2 hours.
• the time period during which the aqueous whey protein solution is kept at the desired temperature ranges from 12 to 25 minutes, more preferably from 12 to 20 minutes, or most preferably about 15 minutes.
• the heat treatment may also be achieved in a microwave oven or any similar equipment allowing heating by microwaves with a time/quantity ratio of between 0.8s per mL and 1.2s per mL of solution. This will depend on the initial temperature of the solution prior to heating and to the power of the microwave oven. For instance, a 4 wt% protein solution heated in a 1500 W apparatus up to boiling temperature (98°C at an altitude of 833m) requires approximately Is per mL of solution.
• Heating the content of the package according to the present invention allows the preparation of a ready-to-eat beverage or liquid comestible while at the same time transforming the native whey protein at least partially into whey protein micelles.
• the solution is heated to a temperature between 80 0 C and 100 0 C, preferably 70 to below 95°C.
• turbidity measurements are an indication of micelle formation.
• the turbidity measured by absorbance at 500nm is at least 3 absorbance units for 1% protein solution but can reach 16 absorbance units when the yield of micellisation is above 80% (see Figure 2) .
• the heated solution will have a milky appearance due to the presence of whey protein micelles.
• Table 1 Physicochemical properties of soluble whey protein aggregates obtained by heat treatment (85 0 C, 15 min) of a 1 wt% protein dispersion in presence or absence of NaCl .
• the conversion yield of native whey protein to micelles decreases when the initial protein concentration of native whey protein is high.
• the yield of formation of whey protein micelles drops from 85% (when starting with 4% proteins) to 50% (when starting with 12% of proteins) .
• the protein concentration is adjusted before heat treatment to manage the optimal whey protein micelles yield.
• native whey protein are converted to whey protein micelles during the heating step.
• the yield of conversion of native whey protein into micelles is of at least 20%, preferably at least 50%, more preferably at least 80%, and the residual soluble aggregates or soluble protein content is preferably below 20%.
• the whey proteins micelles obtained according to the present method have an average diameter of less than l ⁇ m, preferably of from 100 to 900 nm, more preferably from 100 to 700 nm, most preferably from 200-400nm.
• the average micelle size is characterised by a polydispersity index below 0.200.
• An advantage of the method of the present invention is that the whey protein micelles prepared accordingly have not been submitted to any mechanical stress leading to reduction of the particle size during formation. This method induces spontaneous micellisation of whey proteins during heat treatment in the absence of shearing.
• the whey protein micelles have shown to be ideally suited for use as a whitening agent, an emulsifier, a fat substitute, a substitute for micellar casein or a foaming agent, since they are able to stabilize fat and/or air in an aqueous system for prolonged period.
• whey protein micelles may be used as an emulsifying agent, for which the material is ideally suited, since it has a neutral taste and no off-flavour is created by the use of such material. They may also be used as micellar casein substitute.
• the present whey protein micelles may serve as a whitening agent, so that with one compound several tasks may be fulfilled. Since whey is a material abundantly available, the use thereof reduces the cost of a product requiring an emulsifying, filling, whitening or foaming agent, while at the same time adding to its nutritional value. Indeed, the micelles obtained by the present invention have a Protein Efficiency Ratio equivalent to the starting whey protein of at least 100, preferably at least 110, which makes them important nutritional ingredients.
• a package according to the present invention may thus comprise coffee ingredients and whey protein, such that upon heating the whey protein micelles act as a whitening agent.
• the coffee ingredients in the package may be in a dry, soluble state.
• the present whey proteins micelles may be used to increase skimmed milk whiteness and mouthfeel.
• the fat content in a food matrix may be reduced.
• This feature represents a particular advantage of the present whey protein micelles, since it allows e.g. adding a milk creamer without adding additional fat derived from the milk as such.
• the method of the present invention can be used for the preparation of any kind of ready-to-eat beverage or liquid comestible product requiring stabilisation of an emulsion or a foam, such as e.g. cappuccino instant beverages, coffee creamers, or also in low fat or essentially fat free dairy products, or also where whey protein micelles find application as a micellar casein substitute.
• liquid comestible is meant any food product in a liquid or semi-liquid form which can be consumed by a human or an animal.
• the package of the present invention may comprise ingredients selected from coffee, soup ingredients, sauce ingredients, cocoa ingredients, tea ingredients, plant extract ingredients, sweet dessert ingredients etc.
• Examples for products, where the present whey protein micelles may find application are for example food products such as soups, dairy products, pasteurized UHT milk, sweet condensed milk, frappes, fermented milks, milk-based fermented products, milk chocolate, white chocolate, dark chocolate, hot chocolate, sauces, dessert products, cappuccino coffee, coffee creamer, foams, emulsions, fermented cereal based products, infant formula, pet food, liquid oral supplements etc.
• food products such as soups, dairy products, pasteurized UHT milk, sweet condensed milk, frappes, fermented milks, milk-based fermented products, milk chocolate, white chocolate, dark chocolate, hot chocolate, sauces, dessert products, cappuccino coffee, coffee creamer, foams, emulsions, fermented cereal based products, infant formula, pet food, liquid oral supplements etc.
• the present invention provides for an instant food package comprising native whey protein and further beverage or liquid comestible ingredients.
• the whey protein micelles are obtained in situ in an easy step.
• all the benefits associated with whey protein micelles e.g. emulsifier, whitener, stabilizer, nutritional agent etc.
• the cost advantage in using native whey protein instead of expensive emulsifiers, stabilizers etc. is tremendous.
• nutritionally balanced, appealing hot beverages or liquid consumables are easily obtained.
• the ready-to-eat beverage or liquid comestible produced may be cooled prior to consumption.
• it may further be used as an ingredient in the manufacture of other consumables, such as dairy products, mayonnaise, salad dressing, pasteurized UHT milk, sweet condensed milk, yoghurt, fermented milks, milk- based fermented products, milk chocolate, white chocolate, dark chocolate, mousses, foams, emulsions, ice creams, fermented cereal based products, milk based powders, infant formula, diet fortifications, pet food, tablets, liquid bacterial suspensions, dried oral supplement, liquid oral supplement etc.
• the micelles produced during the heat treatment will conserve their characteristics and functions regardless of any cooling or further processing.
• Figures 10 and 11 compare the heat stability of whey protein micelles with native whey protein, whereby the whey protein micelles are noticeably more resistant to heating.
• whey protein micelle concentrate can be easily redispersed in water at room temperature or at 50 0 C.
• the size and structure of the whey protein micelles are fully conserved compared to the initial concentrate. For example, a whey protein micelle concentrate that was spray-dried at
• Example 1 Micellisation of ⁇ -Lactoglobulin ⁇ -Lactoglobulin (lot JE002-8-922, 13-12-2000) was obtained from Davisco (Le Sueur, MN, USA) .
• the protein was purified from sweet whey by ultra-filtration and ion exchange chromatography.
• the composition of the powder is 89.7 % protein, 8.85 % moisture, 1.36% ash (0.079 % Ca 2+ , 0.013 % Mg 2+ , 0.097 % K + , 0.576 % Na + , 0.050 % Cl " ). All other reagents used were of analytical grade (Merck Darmstadt, Germany) .
• the protein solution was prepared at 0.2% concentration by solvation of ⁇ -lactoglobulin in MilliQ® water (Millipore) , and stirring at 20 0 C for 2 h. Then pH of aliquots was adjusted to 5.0, 5.2, 5.4, 5.6, 5.8, 6.0, 6.2, 6.4, 6.6, 6.8, 7.0 by HCl addition.
• the solutions were filled in 20 ml glass vials (Agilent Technologies) and sealed with aluminum capsules containing a silicon/PTFE sealing. The solutions were heated at 85 0 C for 15 min (time to reach the temperature 2.30 - 3.00 min). After the heat treatment, the samples were cooled in ice water to 20 0 C.
• Example 2 Micellisation of whey protein isolate Whey protein isolate (WPI) (Bipro®, Batch JE032-1-420) was obtained from Davisco (Le Sueur, MN, USA) . The composition of the powder is reported in table 2.
• WPI Whey protein isolate
• the protein solution was prepared at 3.4% protein by solvation of whey protein powder in MilliQ® water (Millipore), and stirring at 20 0 C for 2 h.
• the initial pH was 7.2.
• pH of aliquots was adjusted at 5.6, 5.8, 6.0, 6.2, 6.4 and 6.6 by HCl 0. IN addition.
• the solutions were filled in 20 ml glass vials (Agilent Technologies) and sealed with aluminum capsules containing a silicon/PTFE sealing.
• the solutions were heated at 85 0 C for 15 min (time to reach the temperature 2.30 - 2.50 min) . After the heat treatment, samples were cooled in ice water to 20 0 C.
• the turbidity of heated whey proteins has been determined at 500 nm and 25°C, samples were diluted to allow the measurement in the range of 0.1-3 Abs unit (Spectrophotometer Uvikon 810, Kontron Instrument). Values were calculated for the initial protein concentration 3.4%.
• micellisation The pH of micellisation was considered to be reached upon stability (less than 5% variation of the initial value) of the absorbance measured at 500 nm within an interval of 10 minutes for the same sample as illustrated by the figure 2.
• the optimal pH for micellisation was 6.0 to 6.2.
• stable turbidity 21 and residual soluble protein evaluated by absorbance at 280 nm after centrifugation was 1.9%.
• Example 3 Microscopic observation of micelles
• Protein solution was prepared at 2% protein by solvation of whey protein powder (WPI 90 batch 989/2, Lactalis, Retier, France) in MilliQ® water (Millipore) , and stirred at 20 0 C for 2 h. Then pHs of aliquots were adjusted using HCl 0. IN or NaOH 0.1N.
• micellisation The solutions were filled in 20 ml glass vials (Agilent Technologies) and sealed with aluminum capsules containing a silicon/PTFE sealing. The solutions were heated at 85°C for 15 min (time to reach the temperature 2.30-2.50 min) . After the heat treatment, the samples were cooled in ice water to 20 0 C. For this product the optimal pH for micellisation was 7.4.
• TEM micrograph is presented in figure 3. Obtained micelles are presenting a spherical shape with a diameter of 200 nm.
• the intensity-based size distributions of micelles were measured for those micelles obtained by heat-treatment of a 1 wt % ⁇ -lactoglobulin dispersion for 15 min at 85°C at pH 4.25 (positively charged with a zeta potential around +25mV) and at pH 6.0 (negatively charged with a zeta potential around -3OmV) .
• Z-averaged hydrodynamic diameter of the micelles was 229.3 mm at pH 4.25 an 227.2 at pH 6.0.
• ⁇ -LG and whey protein aggregations were followed using dynamic light scattering.
• a Nanosizer ZS apparatus (Malvern Instruments, UK) equipped with a laser emitting at 633 nm and with 4.0 mW power was used.
• the instrument was used in the backscattering configuration, where detection is done at a scattering angle of 173°. This allows considerable reduction of the multiple scattering signals found in turbid samples.
• Samples were placed in a squared quartz cell (Helima, pathlength 1 cm) .
• the path length of the light beam was automatically set by the apparatus, depending on the sample turbidity (attenuation) .
• the autocorrelation function was calculated from the fluctuation of the scattered intensity) .
• the results are presented in figure 6. It shows that the average particle is characterized by a very narrow polydispersity index
• Example 4 Micellisation of a ⁇ -lactoglobulin at a constant pH
• Example 5 Preparing a whitening agent
• Example 6 In-situ formation of whey protein micelles
• Soluble coffee is dry-mixed with instant WPI powder, sucrose and monohydrate sodium dihydrogen phosphate. This blend is then stored in sealed aluminium packs to ensure constant moisture content. 10 g of this dry mix can be dispersed in 90 g of water in a cup and heated in a microwave for 100 s obtain a whey protein enriched milky coffee .
• Soluble coffee is dispersed in water together with instant WPI powder, sucrose and monohydrate sodium dihydrogen phosphate. This liquid blend is then microfiltered and stored at 4°C in polypropylene bottles. 100 mL of this ready to use 3inl coffee blend are poured in a cup and heated in a microwave for 100 s obtain a whey protein enriched milky coffee.

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