WO2007102439A1 - Produit de fractionnement d'extrait aqueux de feuille d'eucommia ulmoides oliver et composition pour ingestion orale comprenant le produit de fractionnement - Google Patents

Produit de fractionnement d'extrait aqueux de feuille d'eucommia ulmoides oliver et composition pour ingestion orale comprenant le produit de fractionnement Download PDF

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Publication number
WO2007102439A1
WO2007102439A1 PCT/JP2007/054086 JP2007054086W WO2007102439A1 WO 2007102439 A1 WO2007102439 A1 WO 2007102439A1 JP 2007054086 W JP2007054086 W JP 2007054086W WO 2007102439 A1 WO2007102439 A1 WO 2007102439A1
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WIPO (PCT)
Prior art keywords
leaf
fraction
extract
food
nakanaka
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PCT/JP2007/054086
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English (en)
Japanese (ja)
Inventor
Tetsuya Hirata
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Kobayashi Pharmaceutical Co., Ltd.
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Publication of WO2007102439A1 publication Critical patent/WO2007102439A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives

Definitions

  • the present invention relates to a water extract fraction of Tochu Nakaba, and an ingestible composition, a pharmaceutical composition, a food composition, a medicine and a food containing the fraction. Furthermore, this invention relates to the manufacturing method of the said fraction.
  • Eucommia ulmoides oliver is a deciduous woody plant that is classified as a genus of the genus Eucommia from Central China, and has a height of 20m. Tonaka is different from the camellia plant, generally called tea, in that it contains no caffeine. Tochu Nakaba has been widely used as a beverage since the 1980s.
  • tea leaves for Tochu tea have been dried by sun-drying the tea leaves harvested in the production area, and then roasting the dried products, usually at 100-140 ° C for 30-50 minutes. However, it was necessary to boil for 10 minutes or more in order to perform sufficient water extraction on the tea leaves produced in this way. Subsequently, after steaming the nakanaka leaves, a method of subjecting them to a twisting process and a roasting process was disclosed, and hot water extraction of the nakanaka leaf components was made possible in a short time (see Patent Document 1).
  • tea leaves for ordinary Tochu tea change color from green to brown in processes such as sun drying and roasting, and the components contained in the tea leaves change accordingly.
  • a method for producing green Tochu tea leaves has also been developed in order to produce green Tochu tea and to contain more raw Tochu leaves in the extract (Patent Documents 2 to (See 4).
  • Patent Document 1 Japanese Patent Application Laid-Open No. 8-173110
  • Patent Document 2 Specification of Patent No. 2775418
  • Patent Document 3 JP-A-11-155537
  • Patent Document 4 JP-A-2005-287469
  • Patent Document 5 JP-A-2005-289950
  • Patent Document 6 JP-A-2005-289951
  • Patent Document 7 Japanese Patent Laid-Open No. 2003-342185
  • Patent Document 8 Japanese Patent Application Laid-Open No. 2002-179586
  • Patent Document 9 Japanese Patent Laid-Open No. 2002-275077
  • Tochu Nakaba As a beverage, a method for producing green Tochu tea leaves intended to retain raw leaf ingredients has been studied! In order to provide beverages, an extraction device or the like is required, and a method for preparing a water extract that can be used to provide Tochu tea more simply has been demanded. Furthermore, the water extract is required to have sufficient medicinal ingredients contained in Tochu Naka, to have a flavor similar to that of Tochu tea prepared by a usual method, and to have excellent storage stability. .
  • the obtained fraction is desired to be easy to handle and have long-term storage stability even when processed as a medicine or food.
  • the present invention provides a method for producing a fraction of a water extract that contains a sufficient amount of components of Tochu Naka, in particular, components of Tochu Tea, and has a flavor similar to that of Tochu Tea when dissolved in water; It is an object of the present invention to provide a fraction of a Tochu leaf ingredient that contains a high concentration of ingredients and is easy to handle during processing; and a beverage containing the fraction.
  • a production method including a step of adsorbing an extract of chunaka leaf water obtained from dried chunaka leaf on a synthetic adsorbent in reverse phase to obtain a component eluted with water.
  • a foliage extract of Tochu Nakahasui is provided.
  • the fraction obtained as a component eluted with water is concentrated. Then, it is provided with a fraction of the Nakanaka leaf water extract already defined as the present invention, which is obtained by a production method further comprising the step of obtaining a powder by drying under reduced pressure.
  • the powder is not particularly limited, but can be obtained, for example, by separating from the tar content.
  • the method for concentrating the fraction is not particularly limited, and can be carried out by methods such as distillation of water by heating under normal pressure and distillation of water under reduced pressure, for example, rotary evaporator. One or a freeze dryer may be used. In the present invention, for example, a certain amount of water is distilled off by heating under normal pressure, and then concentrated by using a rotary evaporator or the like.
  • the content of the asperal mouth side is 1% by weight or less based on the total amount of the solid formation.
  • the Tochu Nakaha extract fraction is provided.
  • the light absorption at 560nm (OD560) in a 0.3 wt% aqueous solution is not more than a certain value, and has already been defined as the present invention.
  • a product fraction is provided.
  • the absorbance may be, for example, 0.06 or less, preferably 0.058 or less, and more preferably 0.055 or less.
  • the absorbance is not particularly limited, but is immediately measured by dissolving the extract of Tochu Nakaba water in distilled water using a spectrophotometer UV-1200 manufactured by Shimadzu Corporation.
  • a production method including a step of drying tsunaka leaf by irradiating infrared rays is provided a tsunaka leaf water extract fraction already defined as the present invention.
  • a medicament comprising the fraction of the Nakanaka leaf water extract already defined as the present invention.
  • the Nakanaka leaf water extract already defined as the present invention A food product comprising the fraction is provided.
  • the food is not particularly limited, and may be, for example, a functional food, a health food, a health supplement, a nutritional supplement, an insurance function food, a specific insurance food, or a function nutrition food.
  • an aqueous solution of the fraction of the Nakanaka leaf water extract already defined as the present invention is provided.
  • the aqueous solution can be used as a beverage, for example.
  • the method includes the step of adsorbing an extract of chunaka leaf water obtained from dried chunaka leaf on a synthetic adsorbent of reverse phase to obtain a component eluted with water.
  • a method for producing a product fraction is provided.
  • the method further includes a step of concentrating the fraction obtained as a component eluted with water and drying under reduced pressure to obtain a powder.
  • the content of the asperal mouth side is 1% by weight or less with respect to the total amount of the solid formation.
  • a solid tsunaka leaf water extract fraction in which the content of the asperal mouth side is 1% by weight or less based on the total amount of the solid component.
  • the fraction of Tochu Nakaha extract contains a sufficient amount of Tochu tea components such as geposide acid and chlorogenic acid, and the aqueous solution has the same flavor as Tochu tea. Furthermore, since the fraction has preferable storage stability, it is useful as a raw material for beverages. In addition, the aqueous solution capable of obtaining the fractional power of the present invention has the characteristics of little change in color tone even when stored for a long period of time! The
  • fraction according to the present invention sufficiently contains the main components of Tochu tea, it is useful in the manufacture of pharmaceuticals and foods for efficiently ingesting the components.
  • the fraction of the present invention includes, for example, an iridoid compound that is a glycoside component contained in Tochu Nakaba.
  • iridoid compound that is a glycoside component contained in Tochu Nakaba.
  • Specific examples thereof include, for example, Geposide acid, Asper mouth side, Asper mouth mouth Examples include side acid (asperulosidic acid), deacetylase spell mouth side acid, scandoside 10 O acetate, oakbin and the like.
  • the chunaka leaf water extract in the present invention is prepared using, for example, dried chunaka leaf.
  • the dry licorice leaf is not particularly limited, but can be prepared, for example, by subjecting licorice leaf to a specific process.
  • the bamboo shoot leaves mean the bamboo leaves before harvest and before drying, and may be produced by cultivation or collected from nature. For example, fresh leaves before fallen leaves of the current year can be used, and fresh leaves can be used from April to October, preferably from May to August, more preferably from July to August.
  • the cocoon leaves of the present invention may be used as they are, or may be cut. Therefore, the present invention may further include a step of cutting the green leaves. ⁇ ⁇ may be cut into a width of, for example, about 5 to 30 mm, preferably about 10 to 20 mm. In the present invention, unbroken cocoon leaves are preferably used. By using the uncut green leaves, it becomes possible to dry gently and gently in the subsequent drying process, and it is possible to suppress the yield drop and the discoloration of the green leaves during the drying. .
  • the steaming step of the cocoon leaves in the present invention can be carried out by a method usually performed in the technical field using a commercially available steamer or an autoclave.
  • a commercially available steamer or an autoclave For example, it is possible to steam heat-treat cocoon leaves by spreading them on a net conveyor and passing them through a treatment chamber filled with non-pressure steam supplied from a boiler.
  • Kashimura Tekko Co., Ltd., leaf feeder, ground type 1500, net conveyor, trolley type 100, etc. can be used.
  • the steaming temperature may be appropriately selected within the range of, for example, 90 to 120 ° C., preferably 95 to 110 ° C., more preferably 100 to 110 ° C., depending on the size of Tochu Nakaba.
  • the steaming time can also be appropriately selected within the range of 10 to 240 seconds, preferably 20 to 180 seconds, more preferably 20 to 120 seconds.
  • the amount of steam used can be appropriately selected within the range of, for example, 200 to 70 LZ, preferably 170 to L00LZ.
  • the treatment amount of the steamed leaves can be appropriately selected in the range of, for example, 3 to: LO kgZ, preferably 4 to 8 kgZ, more preferably 5 to 7 kgZ, depending on the moisture content of the fresh leaves.
  • This steaming process makes it easier to maintain the green color of the Nakanaka leaf by inactivating the enzyme that changes the color of the Nakanaka leaf to brown;
  • the softening has the effect of facilitating the process of drying the steamed Nakanaka leaves after the steaming process with stirring and Z or pressure.
  • the steamed and heat-treated Tochu Nakaba can be used in the next step as it is. It can also be used in the next step after cooling.
  • the cooling here can be performed by removing rough heat by blowing air or the like.
  • the step of drying the bamboo leaf while stirring and Z or under pressure includes, for example, a commercially available continuous leaf cutter, rotary leaf cutter, and batch type leaf cutter. Alternatively, it can be carried out by a method commonly used in the technical field using a roughing machine or the like.
  • a commercially available leaf cutter leaf cutters (batch type) 60K, 90 ⁇ , 120K, and 180K manufactured by Kawasaki Kiko Co., Ltd. can be used.
  • the crushing pressure in this process is performed by a “more hand” supported by a panel panel having an appropriate elasticity rotating in the leaf cutter and pressing the corrugated leaf against the wall of the processing cylinder.
  • the stirring in this step is performed by rotating the “leaf crust” attached to the leaf cutter.
  • the rolling pressure in this step is preferably performed gently, for example, by only rotating the leaves.
  • the drying method is not particularly limited, but is preferably performed by sending hot air to Tochu Nakaba.
  • the temperature of the hot air is not particularly limited, but may be appropriately selected from the range of, for example, 70 to 120 ° C, preferably 75 to 110 ° C.
  • the time required for this step can be appropriately selected within the range of 10 to 60 minutes, preferably 15 to 50 minutes, more preferably 20 to 40 minutes. For example, this step can be performed at 100 ° C. for 20-30 minutes.
  • the surface of the nakanaka leaf can be maintained, the surface temperature of the nakanaka leaf can be kept within a certain range, and drying can be performed while suppressing discoloration of the nakanaka leaf. It becomes possible.
  • the surface temperature of Tochu tea leaves during drying in this step is, for example, 30 to 60 ° C, preferably 40 to 50 ° C. is there.
  • the water content of Tochu Nakaba obtained through this step is, for example, 30 to 45%, preferably 30 to 40%, more preferably 30 to 35% on a dry basis.
  • the present invention includes a step of homogenizing the moisture of the chunaka leaf between "the step of drying the chunaka leaf with stirring and Z or pressure" and "the step of drying the chunaka leaf". It may be.
  • the step of homogenizing the water in the bamboo leaf can be carried out by a method usually performed in the technical field using, for example, a commercially available twister, coarser, or intermediater.
  • a commercially available twister Terada Seisakusho Co., Ltd.
  • a twister 60 kg type can be used as a commercially available twister, Terada Seisakusho Co., Ltd.
  • the homogenization of the water in the cocoon leaf in this process is performed, for example, by sandwiching the cocoon leaf between the turret and the rotating drum of the turret and pressurizing with the rotator while rotating the rotator. Is called. Since the Tochu leaves are dried in the order of mesophyll, vein, and leaf shaft, for example, even if the dryness of the leaf meat is sufficient, excess moisture still remains on the leaf shaft. Therefore, according to this step, the moisture in the bamboo leaf is uniformly prepared, and the powdery wrinkles due to partial drying are suppressed, and at the same time, the time required for drying can be shortened. This step can be performed with heating as required, preferably without heating.
  • the time required for this step can be appropriately selected within the range of 10 to 80 minutes, preferably 20 to 60 minutes, more preferably 30 to 45 minutes.
  • this process can be performed at room temperature for 40 minutes.
  • the force applied by the scissors during this process The no-pressing time at the start of the process can be appropriately selected in the range of 0 to 10 minutes, preferably 2 to 8 minutes, more preferably 4 to 5 minutes. .
  • the moisture content of the chunaka leaf obtained through this step is, for example, 25 to 40%, preferably 25 to 35% on a dry basis.
  • the present invention includes "a step of homogenizing moisture in the chunaka leaf”, an additional “step of drying the chunaka leaf with stirring and Z or under pressure before the step of drying the chunaka leaf. And “the step of homogenizing the water in the Tochu leaf”, or “the step of drying the Tochu leaf with stirring and Z or pressure” may be included. By repeating these steps, it is possible to obtain Tochu tea leaves from which unnecessary lipids have been further removed.
  • the step can be performed by the above-described method and conditions, and the temperature of the hot air is not particularly limited.
  • 50 to 110 A range force of ° C, preferably 55-105 ° C can also be selected as appropriate.
  • the time required for the step can be appropriately selected within the range of 5 to 45 minutes, preferably 10 to 40 minutes, more preferably 10 to 35 minutes. For example, it can be performed at 70 ° C for 20 to 30 minutes.
  • the step can be performed by the above-mentioned method and conditions, and the time required for this step is 10 to 80 minutes, preferably It can be appropriately selected within a range of 20 to 60 minutes, more preferably 30 to 45 minutes. For example, it can be performed at room temperature for 40 minutes.
  • the step of drying the Tochu leaf of the present invention can be carried out, for example, by a method commonly performed in the technical field using a commercially available dryer.
  • the drying method in this process is not specifically limited, For example, it can carry out by moving the Tochu Naka leaf on a conveyance conveyor in the high temperature drying chamber filled with the hot air by a hot air generator.
  • a hot air generator For example, it can be performed by a dryer ND120 manufactured by Terada Manufacturing Co., Ltd.
  • the temperature of the hot air is not particularly limited, but a range force of, for example, 70 to 100 ° C., preferably 85 to 95 ° C. may be appropriately selected.
  • the time required for this step can be appropriately selected within the range of 5 to 80 minutes, preferably 10 to 70 minutes, more preferably 20 to 80 minutes.
  • the water content of the bamboo leaf obtained through this step is, for example, a water content of 5% or less, preferably a water content of 3% or less, more preferably a water content of 2% or less.
  • the present invention may include a step of drying the chunaka leaf by irradiating the chunaka leaf with far infrared rays.
  • this process is not specifically limited, For example, it can be performed using the commercially available far-infrared heater.
  • it can be performed by a VR type manufactured by Yamamasu Seisakusho Co., Ltd.
  • the wavelength of the far infrared rays irradiated here is appropriately selected from the range of, for example, 1 to: LOOO / z m, preferably 2.5 to 50 ⁇ m, more preferably 3 to 30 ⁇ m.
  • the irradiation set temperature during drying can be appropriately selected from the range of, for example, 100 to 400 ° C, preferably 150 to 350 ° C, more preferably 200 to 300 ° C.
  • the time required for this step can be appropriately selected within the range of 30 to 60 seconds, preferably 40 to 55 seconds, and more preferably 45 to 50 seconds as the infrared lamp passing speed.
  • the moisture content of the dried bamboo leaf obtained through this step is, for example, a moisture content of 5% or less, preferably a moisture content of 3% or less, more preferably a moisture content of 2% or less.
  • the Tochu Nakaba water extract is, for example, 5 to 50 k with respect to 1 kg of dried Tochu Nakaba. g, preferably 10-30 kg, more preferably 15-20 kg force
  • An appropriately selected amount of water can be used.
  • the extraction temperature can be appropriately selected from the range of, for example, 85 to 98 ° C, preferably 90 to 95 ° C.
  • the extraction time is not particularly limited, but can be appropriately selected, for example, 10 to 120 minutes, preferably 20 to 90 minutes, more preferably 30 to 60 minutes.
  • Filtration of the extract may be performed using, for example, a filter or a 30-200 mesh filter.
  • the filtrate may be allowed to stand for a certain period before concentration. Unnecessary substances can be removed by removing precipitates generated by standing.
  • the standing time is not particularly limited, but may be appropriately selected from, for example, 1 to 24 hours, preferably 6 to 20 hours, and more preferably 8 to 18 hours.
  • the temperature at the time of standing is not particularly limited. For example, a force of 0 to 35 ° C, preferably 0 to 16 ° C, more preferably 2 to 8 ° C can be appropriately selected.
  • the soot extract obtained in this way can be used as it is, but the supernatant obtained after removing the precipitate generated by centrifugation can also be used in the next step. Further, the supernatant can be used after being concentrated.
  • the "reverse-phase synthetic adsorbent" used in the present invention is not particularly limited as long as it is usually used for separation of natural components, etc., but polystyrene gel (polystyrene 'dibulene benzene copolymer, etc.)
  • polystyrene gel polystyrene 'dibulene benzene copolymer, etc.
  • MCIGEL CHP10M / CHP2MGM SEPABEA
  • the adsorbent can be used as a column carrier, for example.
  • the amount of adsorbent used can be selected, for example, in the range of 10000 to 18000 g, preferably ⁇ 15000 to 18000 g, for 110 L of Tochu leaf extract.
  • a Tochu leaf extract is injected into a column packed with a reverse-phase adsorbent, and then a component that is eluted using a fixed amount of water as an eluent is separated into the components of the present invention. It can be obtained as a picture.
  • the amount of water to be used as the eluent is not particularly limited.
  • a range force of 70 to L 10 L can be used for 17200 g of the adsorbent.
  • the content of the asperal mouth side in the solid component after the solvent is distilled off is, for example, HPLC And the like (the measurement wavelength of absorbance: 215 nm).
  • the content of aucubin (absorbance measurement wavelength: 215 nm), chlorogenic acid (absorbance measurement wavelength: 215 nm) and geposide acid (absorbance measurement wavelength: 215 nm) should be determined in the same manner. Can do.
  • the fraction of the present invention can be obtained as a powder.
  • the content of asperuloside contained in the powder is 1% by weight or less based on the total amount of solid components after the solvent is distilled off. An example is 0.01% by weight or less.
  • the content of Aukubin contained in the powder is 15.0 to 18 against the solid component amount of After evaporation of the solvent. 0 weight 0/0, preferably 16.0 to 18.0 weight 0/0, more preferably 16.4 to 17.4 wt%. An example is 16.9% by weight.
  • the content of chlorogenic acids contained in the powder is from 5.61 to 6.85 wt 0/0 relative to the solid component amount of After evaporation of the solvent, preferably from 5.92 to 6. 54 weight 0/0, more preferably
  • the content of Poshido acid is from 6.50 to 7 94 weight 0/0 relative to the solid component amount of After evaporation of the solvent, preferably 6. 86 to 7.58 weight 0/0, and more preferably
  • the total content of alkvin, chlorogenic acid, and geposide acid contained in the powder is 27.11-32.79 wt% with respect to the total amount of solid components after the solvent is distilled off, Preferably 2
  • the fraction of the present invention can be obtained as a tar-like product.
  • the content of the asper mouth side contained in the tar-like product is 1% by weight or less with respect to the total amount of solid components after the solvent is distilled off, and an example is 0.01% by weight or less.
  • the content of alkvin contained in the tar-like product is 13.5-16. 5% by weight, preferably 14.3-15.8% by weight based on the total amount of solid components after the solvent is distilled off. 0/0, more rather preferably is 14. 6-15. 4 wt%. An example is 15.0% by weight.
  • the content of chlorogenic acids contained in the tar-like product is 14 to 6.28 wt 0/0 5. to solid formation amount total after evaporation of the solvent, preferably 5. 42 to 6.00 weight 0/0, more virtuous It is preferably 5.54-5.88% by weight. An example is 5.7% by weight.
  • gate contained in the tar-like product - The content of Poshido acid is 5. 90-7 22 weight 0/0 to solid formation amount total after evaporation of the solvent, preferably 6. 23-6. 89 weight 0/0, which is the more favorable Mashiku 6. 36-6. 76 wt%. An example is 6.6% by weight.
  • the total amount of alkvin, chlorogenic acid, and geoposide acid contained in the tar-like product is 24.54-300.0% by weight based on the total amount of solid components after the solvent is distilled off, preferably from 25.95 to 28.69 wt 0/0, more preferably from 26.50 to 28.04 wt 0/0.
  • An example is 27.3% by weight.
  • the fraction obtained as an aqueous solution is preferably used after being concentrated.
  • the concentration rate is not particularly limited, but is, for example, 60% or less in volume ratio, preferably 30% or less, and more preferably used as a solid product obtained by drying.
  • the concentration can be performed under normal pressure or reduced pressure, preferably under reduced pressure.
  • the concentration method is not particularly limited as long as it is a commonly used method, and for example, it can be concentrated using a rotary evaporator.
  • the fraction of the present invention can be used as an active ingredient of a pharmaceutical composition having a medicinal effect such as blood pressure lowering or lipase inhibition.
  • the pharmaceutical composition can be used in various dosage forms such as tablets, capsules, powders, granules, pills, solutions, emulsions, suspensions, solutions, spirits, syrups, for oral administration. Extracts, elixirs, and the like can be used. For example, for topical administration, creams, jellies, gels, pastes, ointments, and the like can be used.
  • the pharmaceutical composition may contain various commonly used ingredients, for example, one or more pharmaceutically acceptable excipients, disintegrants, diluents, lubricants, flavors.
  • compositions of the present invention may be in a sustained or sustained release dosage form.
  • the fraction of the present invention can be used, for example, as a component of a tablet.
  • the tablet is produced by a production method usually used in the field of pharmaceutical preparations, and for example, supplemental carotenants such as crystalline cellulose, silicon dioxide, sucrose fatty acid ester, yeast cell wall, glycerin and the like can be used.
  • excipients for example, maltose, dextrin, cyclodex (String, agar, corn protein, tricalcium phosphate, carboxymethylcellulose calcium, vegetable oil, glycerin fatty acid ester, gum arabic
  • the fraction of the present invention preferably contains an excipient.
  • the tablet can be used as a component of the tablet.
  • the tablet is produced by a production method usually used in the field of pharmaceutical preparations.
  • additives such as crystalline cellulose, diacid carbonate, sucrose fatty acid ester, yeast cell wall, and glycerin can be used. .
  • the fraction of the present invention can be used as a powder. Since the powder is granular, it has low viscosity and low hygroscopicity, so that it can be easily encapsulated. In addition, the powder is less likely to be welded or solidified after the packaging material is enclosed. Maintains granularity even at high temperatures (35 ° C) for a long time, for example, 2 weeks.
  • the dose of the anti-obesity agent of the present invention can be appropriately selected depending on the patient's body shape, age, physical condition, degree of disease, elapsed time after onset, etc.
  • the pharmaceutical composition of the present invention is a therapeutic agent.
  • An effective amount and Z or a prophylactically effective amount of an anti-obesity agent can be included.
  • it is generally used in the dose of 10 to 50000 mg / day / adult, preferably 100 to 5000 mg Z day Z adult, as the pulverized mash of the present invention or its water extract.
  • Administration of the pharmaceutical composition can be single dose or multiple doses and can be used in combination with other drugs such as other anti-obesity agents.
  • the food according to the present invention includes a liquid beverage and a solid food.
  • the food can be used as quasi-drugs, other food and drink ingredients, food additives, and the like.
  • the composition for ingestion in this specification can be used as a functional food as it is, and can be used as a component of a pharmaceutical, a quasi-drug, a food and drink, and a food additive.
  • the use enables daily and continuous ingestion of the food or composition for oral consumption having the obesity-improving effect and arteriosclerosis-preventing agent of the present invention, effectively improving obesity, and obesity-related diseases. (Eg hypertension, hyperlipidemia, diabetes) Effective prevention is possible.
  • Examples of foods or beverages containing the anti-obesity agent of the present invention include functional foods, health foods, health supplements, nutritional supplements (nutrient drinks) having a hypotensive effect, a lipase inhibitory effect or an obesity inhibitory effect, etc. Etc.), functional insurance food, food for specified insurance, functional nutrition food, general food (juice, confectionery, processed food, etc.).
  • the food or beverage in this specification includes, as optional additives, inorganic components such as iron and calcium, various vitamins, dietary fibers such as oligosaccharides and chitosan, proteins such as soy extract, and lecithin. Sugars such as lipids, sucrose and lactose can be included.
  • Ion-exchanged water 70 L was added to dry potato Nakaba (10 kg) and heated at 65 ° C for 7 hours. After heating, it was left overnight.
  • Use Zaru Tetron No. 3 to remove the residue from the Nakanaka leaf, and then perform continuous centrifugation! ⁇ (centrifugal acceleration: 9340xg; S-type ultra-high speed centrifuge U— 6— H: Kansai Enshin Separator ), And a supernatant (about 55 L) was obtained.
  • Another batch was performed in the same manner, and the Tochu leaf extract was combined to obtain a solution of about 110 L.
  • the obtained solution was poured into a column (diameter 135x length 1200mm) packed with Diaion HP20.
  • the aqueous solution that also eluted the column force was collected, and then further 70 L of water was used to elute with water.
  • the obtained aqueous solution was concentrated at 65 ° C under reduced pressure (0.5 kPa), and dried to remove the powder generated on the surface of the tar-like material each time.
  • powder fraction A (1930. Og) and tar-like fraction B (2590. Og) were obtained.
  • the obtained solution was concentrated under reduced pressure (0.5 kPa) to 65 ° C until dryness, to obtain a dried kashiwa leaf water extract (652g) as a solid.
  • test solution 1.5 g each of dried kashiwa leaf extract, fraction A and fraction B were weighed, adjusted to 500 g by adding distilled water, and stirred to obtain a test solution.
  • the test solution was sealed in a transparent sealed container and stored at 40 ° C for 2 weeks.
  • the test solution was evaluated by measuring the absorbance at a wavelength of OD560 and OD720 using a visual evaluation of the test solution and a spectrophotometer (UV-1200: manufactured by Shimadzu Corporation).
  • Example 3 The test solution of Example 3 was used as the test solution.
  • the viscosity of the test solution was measured using a digital viscometer (DV— ⁇ +: manufactured by Brook Field) with an ultra-low viscosity UL adapter.
  • Example 3 The test solution of Example 3 was used as the test solution.
  • the taste evaluation of the test solution was measured using a taste sensor 1 (taste recognition device SA402B: manufactured by Incent Corporation).
  • Example 3 The test solution of Example 3 was used as the test solution.
  • the components contained in the test solution were a ucubin, asperuloside, geniposidic acid and chlorogenic acid as standard substances.
  • FIG. 1 is a diagram showing an example of a taste test result of an aqueous solution of Tochu Nakaha water extract.

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Abstract

Le problème à résoudre dans le cadre de cette invention concerne l'obtention d'un procédé de fractionnement permettant d'extraire un ingrédient, en particulier un ingrédient actif sur le plan pharmacologique, tel qu'un composé iridoïde, d'une feuille d'Eucommia ulmoides oliver présentant une efficacité satisfaisante; un produit de fractionnement d'une feuille d'Eucommia ulmoides oliver, ledit produit de fractionnement contenant un composé spécifique en grande concentration; et une composition pour ingestion orale, composition pharmaceutique, composition alimentaire, un produit pharmaceutique ou alimentaire comprenant une matière naturelle comme matière première, entraînant peu d'effets secondaires indésirables, et sans danger même lorsque pris pendant une période prolongée. La solution proposée consiste à obtenir un produit de fractionnement d'extrait aqueux d'une feuille d'Eucommia ulmoides oliver. Le produit de fractionnement peut être obtenu par un procédé comprenant l'étape d'adsorption d'un extrait aqueux d'une feuille d'Eucommia ulmoides oliver sur un adsorbant synthétique de phase inversée de façon à obtenir un composant qui peut être élué avec de l'eau, selon lequel l'extrait aqueux est préparé à partir d'une feuille séchée d'Eucommia ulmoides oliver. L'invention concerne également un produit pharmaceutique ou alimentaire comprenant le produit de fractionnement.
PCT/JP2007/054086 2006-03-03 2007-03-02 Produit de fractionnement d'extrait aqueux de feuille d'eucommia ulmoides oliver et composition pour ingestion orale comprenant le produit de fractionnement WO2007102439A1 (fr)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010065960A2 (fr) 2008-12-05 2010-06-10 Semprus Biosciences Corp. Compositions pour greffe de type « à partir de » anti-thrombogènes, antimicrobiennes et anti-encrassement
JP2010273569A (ja) * 2009-05-26 2010-12-09 Wasaburo Sato メタボリックシンドローム改善作用を有する飲料及びサプリメント
CN102670791A (zh) * 2012-06-04 2012-09-19 黄芸 缓解高血压杜仲中药口服液及制备方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5279402B2 (ja) * 2008-08-11 2013-09-04 小林製薬株式会社 杜仲葉乾燥物から調製される抽出液、該抽出液の食品加工物及び杜仲葉乾燥物
JP5561984B2 (ja) * 2009-09-29 2014-07-30 小林製薬株式会社 新規飲料
JP5323003B2 (ja) * 2010-06-01 2013-10-23 有限会社 碧山園 杜仲葉緑色乾燥品の製造方法
JP5766173B2 (ja) * 2012-12-14 2015-08-19 小林製薬株式会社 クロロゲン酸類を含有する飲食品
CN109045098A (zh) * 2018-07-11 2018-12-21 贵州神奇药业有限公司 一种降三高含片及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04368336A (ja) * 1991-06-18 1992-12-21 Hitachi Zosen Corp 杜仲葉エキス由来のアンジオテンシン転換酵素活性阻害剤
JPH09248163A (ja) * 1996-03-14 1997-09-22 Hitachi Zosen Corp 味品質を確保した杜仲茶
JPH10304848A (ja) * 1997-05-07 1998-11-17 Hitachi Zosen Corp 抗高脂血症機能を持つ食品
WO2005099488A1 (fr) * 2004-03-31 2005-10-27 Kobayashi Pharmaceutical Co., Ltd. Feuilles de thé vert de du-zhong et procédé de fabrication d'une poudre de feuilles de thé vert de du-zhong
WO2005099734A1 (fr) * 2004-03-31 2005-10-27 Kobayashi Pharmaceutical Co., Ltd. Inhibiteur de lipase contenant un extrait aqueux provenant d'une feuille d'arbre à caoutchouc vivace

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04368336A (ja) * 1991-06-18 1992-12-21 Hitachi Zosen Corp 杜仲葉エキス由来のアンジオテンシン転換酵素活性阻害剤
JPH09248163A (ja) * 1996-03-14 1997-09-22 Hitachi Zosen Corp 味品質を確保した杜仲茶
JPH10304848A (ja) * 1997-05-07 1998-11-17 Hitachi Zosen Corp 抗高脂血症機能を持つ食品
WO2005099488A1 (fr) * 2004-03-31 2005-10-27 Kobayashi Pharmaceutical Co., Ltd. Feuilles de thé vert de du-zhong et procédé de fabrication d'une poudre de feuilles de thé vert de du-zhong
WO2005099734A1 (fr) * 2004-03-31 2005-10-27 Kobayashi Pharmaceutical Co., Ltd. Inhibiteur de lipase contenant un extrait aqueux provenant d'une feuille d'arbre à caoutchouc vivace

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAKAZATO M. ET AL.: "Tochuba o Shugenryo to shita Kenko Shokuhinchu no Geniposide san Oyobi Caffeine no Bunseki", SHOKUHIN EISEIGAKU ZASSHI, vol. 37, no. 6, 1996, pages 343 - 350, XP003017655 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010065960A2 (fr) 2008-12-05 2010-06-10 Semprus Biosciences Corp. Compositions pour greffe de type « à partir de » anti-thrombogènes, antimicrobiennes et anti-encrassement
JP2010273569A (ja) * 2009-05-26 2010-12-09 Wasaburo Sato メタボリックシンドローム改善作用を有する飲料及びサプリメント
CN102670791A (zh) * 2012-06-04 2012-09-19 黄芸 缓解高血压杜仲中药口服液及制备方法

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