WO2007030974A1 - Utilisation d'heteropolysaccharide de tremella fuciformis ou d'extrait correspondant - Google Patents

Utilisation d'heteropolysaccharide de tremella fuciformis ou d'extrait correspondant Download PDF

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Publication number
WO2007030974A1
WO2007030974A1 PCT/CN2005/001573 CN2005001573W WO2007030974A1 WO 2007030974 A1 WO2007030974 A1 WO 2007030974A1 CN 2005001573 W CN2005001573 W CN 2005001573W WO 2007030974 A1 WO2007030974 A1 WO 2007030974A1
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WIPO (PCT)
Prior art keywords
tremella
heteropolysaccharide
extract
food
capsules
Prior art date
Application number
PCT/CN2005/001573
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English (en)
Chinese (zh)
Inventor
Guiyun Liu
Feng Luo
Bin Luo
Original Assignee
Shanghai Wenda Biotech Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Wenda Biotech Inc. filed Critical Shanghai Wenda Biotech Inc.
Publication of WO2007030974A1 publication Critical patent/WO2007030974A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants

Definitions

  • the present invention relates to the use of a heteropolysaccharide, and more particularly to the use of a Tremella heteropolysaccharide or an extract thereof for protecting or repairing a gastric mucosa.
  • Tremella faciformis Berk also known as white fungus, mulberry goose, and Qing dynasty. It belongs to TremelKles, Tremellacease, and Tremella. It has the effects of nourishing yin and moistening the lungs, benefiting Qi and blood.
  • Tremella fuciformis is an acidic heteropolysaccharide extracted from the fungus fruit body (or its microbial fermentation broth). Since ancient times, China has called white fungus high-grade supplements and medicines. The best for medicine and food is the court's finest tonic food.
  • Tremella fuciformis which indicates that Tremella fuciformis has improved immunity and resistance. Tumor, anti-aging, blood sugar lowering, blood lipids, protein nucleic acid synthesis, antithrombotic and other pharmacological effects. Japan has applied it extensively to health products, cosmetics, and pharmaceuticals.
  • the development and application of Tremella has attracted great interest from the scientific community. It has received extensive attention in the international pharmaceutical and cosmetic fields and has become a hot topic in research and development.
  • Tremella polysaccharides and the corresponding functions have rarely been reported so far.
  • the applicant uses a special separation and purification process to refine the active polysaccharide in Tremella fuciformis, which is identified by modern analytical techniques.
  • the structure of the Tremella heteropolysaccharide is ⁇ - ( 1-3 ) mannose as the main chain, mainly by weight percentage 70- 93% neutral total sugar, 6-28% gluconic acid, 0.1-3% binding protein; the average molecular weight of the Tremella heteropolysaccharide is 85-1.6 million Daltons, of which the molecular weight is greater than 6000 Daltons 90% (by weight) or more, as described in the patent application No.
  • the object of the present invention is to provide a novel use of the Tremella heteropolysaccharide or Tremella polysaccharide extract of the trade name WSK in the patent application No. 200510023427.4, which is a Tremella heteropolysaccharide or a Tremella heteropolysaccharide
  • the structure of the Tremella heteropolysaccharide according to the present invention is ⁇ -(1-3) mannose as the main chain, mainly consisting of 70-93% by weight of neutral total sugar, 6-28% of glucuronic acid, 0.1- 3% of the binding protein composition; the average molecular weight of the Tremella heteropolysaccharide is 85-1.6 million Daltons, wherein more than 90% by weight of the molecular weight is greater than 6000 Daltons.
  • the neutral total sugar mainly comprises the following content of glycosyl: 35-60% mannose, 10-25% fucose, 8-18% xylose and 5-15% glucose, the above content is white fungus Heteropolysaccharide weight meter.
  • the side chain of the tremella heteropolysaccharide has a plurality of different combinations of changes, one of which has been determined by the X-ray diffraction method as a structural unit represented by the following structural formula I -
  • the structural unit has a molecular weight of 2578.5 or 2724.7.
  • the sugar structure is composed of ⁇ -(1-3) mannose (Manp) as the main chain skeleton, and two ⁇ -(1-2) xylose (Xylp) and three ⁇ -glucose are attached to the two carbon atoms.
  • Aldehydic acid (GlcAp), 1 or 2 fucose (Fuc), 1 mannose is a side chain.
  • the Tremella heteropolysaccharide extract of the present invention mainly comprises 80-98% by weight of the above-mentioned Tremella heteropolysaccharide, 0.1-3.3% of free protein and 0.5-8% of ash; the average molecular weight of the Tremella heteropolysaccharide extract is 75-1.4 million Daltons, of which 75-98% by weight of molecular weight greater than 6000 Daltons.
  • the preparation method of the Tremella heteropolysaccharide extract of the present invention comprises the following steps:
  • step 2) filtering the crude extract obtained in step 1), and finally passing the filtrate through a filter having a pore diameter of 0.1-1 ⁇ m; 3) adding ethanol to the filtrate obtained in the step 2) to make the final volume concentration of ethanol 50-85%, collecting the precipitate;
  • the precipitate obtained in the step 3) is dried.
  • the raw material of the white fungus fruit body is a dry raw material of the tremella fruit body, and the dried tremella fruit material is soaked, expanded and cleaned with water (including a yellow-brown base), 20-100 times each time, and multiple times of extraction. Not more than 200 times by weight of the dried fermented fruit body material amount of water is stirred and extracted at 80-100 ° C to obtain a crude extract.
  • the raw material of the white fungus fruit body may also be a raw material of fresh white fungus fruit body, fresh frozen fungus fruit raw material or frozen fresh white fungus fruit material, and the raw material of the white fungus fruit body is removed to a yellow-brown base and cleaned, and each time 2- 15 times, multiple extractions, no more than 30 times (by weight) of the amount of water of the raw material of the fungus fruit body is stirred and extracted at 80-100 Torr to obtain a crude extract.
  • Another preparation method of the Tremella heteropolysaccharide extract of the present invention comprises the following steps:
  • the preparation method of the Tremella heteropolysaccharide according to the present invention comprises the step of further purifying the extract prepared by the above preparation method.
  • the purified hydrazine is achieved by the Sevag method and the dialysis method.
  • other polysaccharide purification methods such as chromatographic separation methods, can also produce the polysaccharides of the present invention.
  • the pharmaceutical preparations may be in various dosage forms of the prior art, such as powders, tablets, capsules (including soft and hard capsules), liquids, suspensions, emulsions, oral solutions, jelly or granules.
  • the food or drink comprises a drink and a food, and is a drink or a food containing a concentrate and/or a dried product of a tremella heteropolysaccharide or a tremella heteropolysaccharide extract, and the application of the food or drink is not limited, and may be each of the prior art.
  • a dosage form such as It can be used in powders, tablets, capsules (including soft and hard capsules), granules, granules, jelly, beverages, dairy products or canapes.
  • the health care product may be in various dosage forms of the prior art, such as powders, tablets, capsules (including soft and hard capsules), liquids, suspensions, emulsions, oral liquids, jelly or granules, and the like. .
  • the medicine may further contain other drugs for protecting or repairing the gastric mucosa, and the white fungus heteropolysaccharide may serve to assist in protecting or repairing the gastric mucosa.
  • Other drugs that protect or repair the gastric mucosa can be: such as nabumetone, which inhibits gastric mucosal neutrophil activation.
  • the food or drink or health supplement may also contain various other edible nutrients for better nutrition and health care.
  • the Tremella heteropolysaccharide may be crude crude tremella heteropolysaccharide containing impurities or pure tremella heteropolysaccharide.
  • the protection of the gastric mucosa refers to the prevention of damage to the gastric mucosa or the reduction of damage to the gastric mucosa.
  • the repair of the gastric mucosa refers to repairing the damaged gastric mucosa to restore the damaged gastric mucosa to a normal state of health. In the process of repairing the gastric mucosa, the gastric mucosal epithelial cells are regenerated, the defense function is enhanced, the ability to resist pathogenic factors is improved, and the barrier function of the gastric mucosa is enhanced.
  • Tremella polysaccharide extract and Tremella fuciformis are prepared according to the preparation method of the applicant's patent application No. 200510023427.4, which is described in detail as follows:
  • the Tremella fermentation broth obtained by microbial fermentation is heated at 100 ° C for 0.5 h, filtered conventionally, and the filter residue is discarded.
  • the supernatant is finally passed through a filter having a pore diameter of 0.1-1 ⁇ m; the filtrate and the ethanol are thoroughly mixed under uniform stirring.
  • the final concentration of ethanol in the mixed liquid was 85%, the precipitate was collected, dehydrated by dehydration with 100% ethanol, and the precipitate was collected; freeze-dried at -40 ° C, and pulverized to obtain a white fungus heteropolysaccharide extract.
  • Product yield 0.55% (based on the weight of the fermentation broth).
  • aqueous phase was concentrated under reduced pressure to one-third, and the concentrate was thoroughly mixed with 3 times (volume ratio) of 95% ethanol in the aqueous phase, and centrifuged at 4000 r/min for 30 minutes to obtain a precipitate.
  • the precipitate was dissolved in 100 times by weight of deionized water, centrifuged, and insoluble matter was added.
  • the supernatant was placed in a 30 cm long dialysis bag (the dialysis bag was passed through 5000 molecular weight or less) for dialysis (the two ends were tied and hanged)
  • the deionized water bottle was rinsed with deionized water for 12 hours), and 5000 molecular weight or higher substances were collected.
  • the dialyzed supernatant was thoroughly mixed with 3 times by volume of pure ethanol, and centrifuged again at 4000 r/min for 30 minutes to obtain a precipitate.
  • the precipitate was dehydrated with absolute ethanol and dried under vacuum at 60 Torr to obtain pure silver fungus heteropolysaccharide (WSK). ).
  • Tremella heteropolysaccharide obtained in Example 4, based on an adult weighing 60 kg, recommended a daily dose of -0.8% (w/w) Tremella heteropolysaccharide solution, 200 mL/person, twice a day. Rats were dosed at 0.8% (w/w) Tremella heteropolysaccharide 10 times the human dose, S ⁇ 6.6 mL/200 g twice daily.
  • Instruments and reagents Anatomical instruments, vernier calipers, alcohol.
  • Animal test substance administration route Each dose was 6.6 mL/rat, and the stomach was administered twice.
  • mice in the experimental group were given 0.8% (w/w) Tremella heteropolysaccharide solution (administered twice a day) for 10 consecutive days, and were intragastrically irrigated by the stomach; 10 rats of the control group Normally incubated for 10 days (different distilled water twice a day), the rats were fasted (not allowed to water) for 24 hours before dissection.
  • the experimental group was intragastrically administered with 0.8% (w/w) 5 times dose (3.3 mL) of Tremella fuciformis.
  • the control group was given the same amount of distilled water on the same day. After 1 hour, the ethanol was re-administered (dose was 1.0 mL).
  • the animal was sacrificed; the stomach was fixed in 10% formaldehyde; after 20 minutes, it was cut along the stomach, the stomach contents were removed, the gastric mucosa was unfolded, and the congestion was measured with a vernier caliper. Or the area of the diffuse bleeding zone (accurate to 0.01mm). The total area of the congestive zone and the diffuse hemorrhagic zone was used as an indicator of the degree of injury. .
  • Tremella fuciformis extract and Tremella fuciformis have preventive and protective functions against gastric mucosal damage, such as protective function against acute gastric mucosal damage, especially for acute alcohol damage of gastric mucosa.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
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  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Abstract

Utilisation d'hétéropolysaccharide de Tremella fuciformis ou d'extrait correspondant pour l'élaboration de médicaments, d'aliments ou d'aliments fonctionnels permettant d'assurer la protection ou la restauration de la muqueuse gastrique.
PCT/CN2005/001573 2005-09-16 2005-09-26 Utilisation d'heteropolysaccharide de tremella fuciformis ou d'extrait correspondant WO2007030974A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CNB2005100297466A CN100490821C (zh) 2005-09-16 2005-09-16 银耳杂多糖或其提取物的用途
CN200510029746.6 2005-09-16

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WO2007030974A1 true WO2007030974A1 (fr) 2007-03-22

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103819571A (zh) * 2013-12-20 2014-05-28 中华全国供销合作总社南京野生植物综合利用研究所 一种银耳子实体多糖制备技术

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102219932B (zh) * 2011-05-06 2013-06-12 齐齐哈尔大学 含有银耳多糖的可食性汤料包装膜的制备方法
CN102161710A (zh) * 2011-05-16 2011-08-24 高其品 低分子量银耳多糖的制备方法及其医药新用途
CN102499407B (zh) * 2011-11-15 2013-02-13 北川丰煜食品综合加工进出口有限公司 猕猴桃饮料及其制备方法
CN108968074B (zh) * 2018-06-06 2021-09-28 福建省农业科学院农业工程技术研究所 一种结肠靶向递送的银耳糖醛酸多糖-芙蓉李花青素复合物及其制备方法

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1415630A (zh) * 2002-11-27 2003-05-07 高其品 银耳多糖，其制备方法和以该化合物为活性成分的药物组合物

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1415630A (zh) * 2002-11-27 2003-05-07 高其品 银耳多糖，其制备方法和以该化合物为活性成分的药物组合物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PHARMACOLOGY AND CLINIC OF TCM, vol. 16, no. 4, 2000, pages 44 - 46 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103819571A (zh) * 2013-12-20 2014-05-28 中华全国供销合作总社南京野生植物综合利用研究所 一种银耳子实体多糖制备技术
CN103819571B (zh) * 2013-12-20 2016-08-17 中华全国供销合作总社南京野生植物综合利用研究所 一种银耳子实体多糖制备技术

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