WO2006080210A1 - Réactif, procédé et ensemble pour améliorer l'efficacité d'ionisation et leur utilisation - Google Patents

Réactif, procédé et ensemble pour améliorer l'efficacité d'ionisation et leur utilisation Download PDF

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Publication number
WO2006080210A1
WO2006080210A1 PCT/JP2006/300554 JP2006300554W WO2006080210A1 WO 2006080210 A1 WO2006080210 A1 WO 2006080210A1 JP 2006300554 W JP2006300554 W JP 2006300554W WO 2006080210 A1 WO2006080210 A1 WO 2006080210A1
Authority
WO
WIPO (PCT)
Prior art keywords
metal complex
protein
metal
kit
ligand
Prior art date
Application number
PCT/JP2006/300554
Other languages
English (en)
Japanese (ja)
Inventor
Shin-Ichiro Nishimura
Noriko Nagahori
Original Assignee
National University Corporation Hokkaido University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation Hokkaido University filed Critical National University Corporation Hokkaido University
Priority to JP2007500466A priority Critical patent/JPWO2006080210A1/ja
Publication of WO2006080210A1 publication Critical patent/WO2006080210A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
    • C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
    • C07F15/0086—Platinum compounds
    • C07F15/0093—Platinum compounds without a metal-carbon linkage

Definitions

  • the method for improving protein ion efficiency according to the present invention is characterized by including a step of introducing an organic residue derivative onto the surface of a target protein.
  • R is preferably H or quaternary ammonia
  • X is preferably Pt or Cu.
  • composition is preferably used to improve detection sensitivity in mass spectrometry.
  • the metal complex having the functional group of the present invention can be synthesized by first preparing a ligand having the functional group and then coordinating the ligand to the metal.
  • the present invention also includes a protein analysis method for determining the presence or absence of a histidine residue, an arginine residue, and / or a cysteine residue in a protein using the above metal complex or composition.
  • the metal complex has the following formula:

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electron Tubes For Measurement (AREA)

Abstract

La présente invention concerne une protéine qui est modifiée dans des conditions modérées pour améliorer l’efficacité d’ionisation de la protéine. Un complexe métallique comprenant un groupe fonctionnel spécifique et un ligand tridenté est lié à une protéine cible. Ainsi, la protéine est détectée avec une sensibilité élevée indépendamment de la nature de la protéine.
PCT/JP2006/300554 2005-01-27 2006-01-17 Réactif, procédé et ensemble pour améliorer l'efficacité d'ionisation et leur utilisation WO2006080210A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2007500466A JPWO2006080210A1 (ja) 2005-01-27 2006-01-17 イオン化効率を向上させるための試薬、方法およびキット、並びにその利用

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005019516 2005-01-27
JP2005-019516 2005-01-27

Publications (1)

Publication Number Publication Date
WO2006080210A1 true WO2006080210A1 (fr) 2006-08-03

Family

ID=36740244

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2006/300554 WO2006080210A1 (fr) 2005-01-27 2006-01-17 Réactif, procédé et ensemble pour améliorer l'efficacité d'ionisation et leur utilisation

Country Status (2)

Country Link
JP (1) JPWO2006080210A1 (fr)
WO (1) WO2006080210A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103044462A (zh) * 2012-11-20 2013-04-17 复旦大学 钳型Cu（II）金属有机络合物小分子水凝胶及其应用
JP2015524058A (ja) * 2012-05-22 2015-08-20 バイオジェン・エムエイ・インコーポレイテッドＢｉｏｇｅｎ ＭＡ Ｉｎｃ． 反応性代謝物質のスクリーニングのための捕獲剤

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200052329A (ko) * 2017-09-08 2020-05-14 에프. 호프만-라 로슈 아게 질량 분석법용의 설폭사이드 기반 시약

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000226449A (ja) * 1999-02-05 2000-08-15 Agency Of Ind Science & Technol アルキル置換フェノール重合体の製造方法
JP2004045292A (ja) * 2002-07-12 2004-02-12 Shimadzu Corp 新規な金属錯体、及びそれを用いるタンパク質のアミノ酸配列決定方法
JP2004075633A (ja) * 2002-08-21 2004-03-11 Asahi Kasei Chemicals Corp ヘテロポリ酸塩の製造方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000226449A (ja) * 1999-02-05 2000-08-15 Agency Of Ind Science & Technol アルキル置換フェノール重合体の製造方法
JP2004045292A (ja) * 2002-07-12 2004-02-12 Shimadzu Corp 新規な金属錯体、及びそれを用いるタンパク質のアミノ酸配列決定方法
JP2004075633A (ja) * 2002-08-21 2004-03-11 Asahi Kasei Chemicals Corp ヘテロポリ酸塩の製造方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015524058A (ja) * 2012-05-22 2015-08-20 バイオジェン・エムエイ・インコーポレイテッドＢｉｏｇｅｎ ＭＡ Ｉｎｃ． 反応性代謝物質のスクリーニングのための捕獲剤
US10100081B2 (en) 2012-05-22 2018-10-16 Biogen Ma Inc. Trapping reagents for reactive metabolites screening
CN103044462A (zh) * 2012-11-20 2013-04-17 复旦大学 钳型Cu（II）金属有机络合物小分子水凝胶及其应用

Also Published As

Publication number Publication date
JPWO2006080210A1 (ja) 2008-06-19

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